MD07G1291400.v1.1

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000119
Physical Location & Seq
Reverse (-)
35214300 .. 35217859
3560 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1291400.v1.1.491

Sequence Viewer

Length: 1869 bp
ATGATGCATGTCCCGAATAATGAGTCCACCATTGAATTAAGCCAAACACCTTTCGATCCCAACAGACTTAACCTGCAACCTGGAGGAGCTTCACCTAGTACAAAATTTAAAAGGCCAAAACTGGATCGACTTCTTGCAAATGATCTACTGTGGAACAAAATATTTGTAATTTTATGTGTGATTGCTGTGTCACTGGACCCTTTGTTCTTTTACATTCCATTCATCAATGAAAAAAACAAGTGCCTTGGAATGGACAAGAAGCTGAAGAATGTAACTCTTGTTTTGCGACTGCTCACAGATCTCATTTTCGTAGTGCATATTATTCATCAAATTCGTCAGGCCATTAAATCTGTCAACTCACGTGGAATGGAAAATCCCAGTTGGGAATCGAAAGCTGTGGCAGTAGCCAGGAAGTTGTCGTGGCGCTCGATCGTTACTGACATTCTTGCTGTTCTTCCCATCCCACAAGTGCTTTTAGTACATGTTTTCTTCAAAATGACAGGCTATCGATATTTGTACAAAAGAAAGGTTCTAACTTTTTTCCTTCTTGCCCAATATCTTCCGAGGATTTATCGATTTCTCCTATCCTCTAACAACCTTCCACAAACTGGAATATGGACCAAAGGTGTATTCTATTTTGTCCTTTACATCCTTGCAAGTCATGTACTTGGAGCTTTTTGGTACTTTTTTTCTATTCAACGAGAGACATCATGTTGGTATCGGACATTTAGAAAACATAACATGACAGACTTGAGTACTTTTTACTGTGACGACAGTGCTACCCCAAGAAATATTACATCATTTCTAGATAAATTTTGTCCAATAAATGTTCCTGAAAATACTACACGGCCGTTTGATTTCGGAATATTTCTTGATTCTCTTCGATCTGGAAACGCGGCGTCGTTGGATTTCCCGGGCAAGTTGTTCTACTCGTTTTGGTGGGGACTGCGAAATTTAAGCAACTTTGGCACAAATCTGACAACAAGTACATATGTGTGGGAAAACATCTTTGCAATTCTTATTTCTATCATTGGATTACTCCTATTTTTATATCTCATAGGAAATGTACAGACGTTTATGCAATTGGCAACTACAAGGTCGGAGGATATAAGACAGAAGATTAAGATGAAAGAGCGACAAATAGAAGAGTGGATGGTGAAAAATGATCTCCCAGAAGATTTGAAAAAAGAGATCAAGAAAAACGTAAAACAAAAACTGAAAGAAAACAAAGATGCTGATCTGGAGAATCTGTTCTCCATTCTTCCTTGGTACACCAAGAAACCCCTAAAGCGTTTTCTCTTCATGAATACCCTAAGGACAGTACCAATGCTCAAAGAAATGGATGAAAAAGTGTTGAAAATGATCTGCGACTATCTGAGGCCAGTGACGTACGACGAGAATAGCTTCGTTGTGCGAACAGGAGACCTTCTCGATCGGATGATCTTCATTACGGAAGGGTTTATATGGACTTATGTGGGCGGTGATCAGAATCGACATGGTGGTGGATCATCATCAGGTTCTCAATCCTCATCAATGCCAACCAACATGCTTAAGAAAGGCCAGTTCTACGGCGAAGAGCTTCTCTACCGGCAGTCATCTCCGTCGCAACTTCCAATCTCCCCACAAACCGTTAAATGCCATACAAAAGTCGAAGCTTTTGTTCTTATGGCCAAGGACTGGACAAGTATAGTCTCCAAATGCAGATCGTTGTTGCCGAACAACATGAACCACGGCAACAACAACAACAACAACAACAACAACAATATGAACGTTACTCATTATGGACAGCCCTTACTGAGGGGCTTCAAGAGCTCTACGGCCTCGACACCAGGAGTCCGGTATTTCGAACCTGCTATGCGCCCCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

623

Amino Acids

72.01

Weight (kDa)

9.62

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 81, 1858
AccB7I CCANNNNNTGG 2 cut(s) 608, 1677
AccII CGCG 1 cut(s) 896
AciI CCGC 2 cut(s) 896, 1479
AclI AACGTT 1 cut(s) 1770
AclWI GGATC 3 cut(s) 50, 132, 1513
AcoI YGGCCR 2 cut(s) 848, 1668
AcsI RAATTY 4 cut(s) 104, 330, 812, 952
AcuI CTGAAG 1 cut(s) 284
AcvI CACGTG 1 cut(s) 362
AcyI GRCGYC 1 cut(s) 899
AfiI CCNNNNNNNGG 4 cut(s) 250, 608, 1677, 1797
AflII CTTAAG 1 cut(s) 1550
AflIII ACRYGT 1 cut(s) 481
AgsI TTSAA 6 cut(s) 35, 493, 698, 1183, 1357, 1807
AjnI CCWGG 3 cut(s) 79, 407, 1828
AjuI GAANNNNNNNTTGG 2 cut(s) 614, 646
AloI GAACNNNNNNTCC 4 cut(s) 188, 220, 1235, 1267
AluBI AGCT 8 cut(s) 89, 262, 395, 674, 1404, 1579, 1655, 1812
AluI AGCT 8 cut(s) 89, 262, 395, 674, 1404, 1579, 1655, 1812
Alw21I GWGCWC 1 cut(s) 1814
Alw26I GTCTC 3 cut(s) 698, 1416, 1696
AlwI GGATC 3 cut(s) 50, 132, 1513
Ama87I CYCGRG 1 cut(s) 913
AoxI GGCC 7 cut(s) 113, 339, 848, 1379, 1558, 1668, 1818
ApoI RAATTY 4 cut(s) 104, 330, 812, 952
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
Asp700I GAANNNNTTC 3 cut(s) 1250, 1403, 1578
AspLEI GCGC 2 cut(s) 426, 1860
AspS9I GGNCC 2 cut(s) 196, 618
AsuC2I CCSGG 2 cut(s) 914, 915
AsuHPI GGTGA 3 cut(s) 84, 1168, 1493
AsuII TTCGAA 1 cut(s) 1845
AvaI CYCGRG 1 cut(s) 913
AvaII GGWCC 2 cut(s) 196, 618
AxyI CCTNAGG 1 cut(s) 1313
BalI TGGCCA 1 cut(s) 1670
BanII GRGCYC 1 cut(s) 1814
BbrPI CACGTG 1 cut(s) 362
Bbv12I GWGCWC 1 cut(s) 1814
BccI CCATC 2 cut(s) 467, 1147
BceAI ACGGC 5 cut(s) 835, 863, 1585, 1747, 1833
BciT130I CCWGG 3 cut(s) 81, 409, 1830
BclI TGATCA 1 cut(s) 1483
BcnI CCSGG 2 cut(s) 914, 915
BcoDI GTCTC 3 cut(s) 698, 1416, 1696
BfaI CTAG 2 cut(s) 96, 806
BfoI RGCGCY 1 cut(s) 427
BfrI CTTAAG 1 cut(s) 1550
BfuAI ACCTGC 2 cut(s) 81, 1858
BglII AGATCT 1 cut(s) 298
BisI GCNGC 1 cut(s) 897
BlsI GCNGC 1 cut(s) 898
BmcAI AGTACT 1 cut(s) 757
Bme1390I CCNGG 5 cut(s) 81, 409, 914, 915, 1830
Bme18I GGWCC 2 cut(s) 196, 618
BmeT110I CYCGRG 1 cut(s) 913
BmgT120I GGNCC 2 cut(s) 196, 618
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 5 cut(s) 81, 409, 914, 915, 1830
BmrI ACTGGG 1 cut(s) 372
BmsI GCATC 1 cut(s) 1222
BmuI ACTGGG 1 cut(s) 372
BplI GAGNNNNNCTC 2 cut(s) 1413, 1445
BpmI CTGGAG 2 cut(s) 102, 1262
Bpu14I TTCGAA 1 cut(s) 1845
BpuEI CTTGAG 1 cut(s) 772
BpuMI CCSGG 2 cut(s) 914, 915
Bsa29I ATCGAT 2 cut(s) 508, 574
BsaAI YACGTR 1 cut(s) 362
BsaBI GATNNNNATC 3 cut(s) 1236, 1488, 1510
BsaHI GRCGYC 1 cut(s) 899
BsaI GGTCTC 1 cut(s) 1416
BsaJI CCNNGG 6 cut(s) 244, 563, 913, 1265, 1671, 1729
BsaWI WCCGGW 1 cut(s) 1836
BsaXI ACNNNNNCTCC 2 cut(s) 1235, 1265
Bsc4I CCNNNNNNNGG 4 cut(s) 250, 608, 1677, 1797
Bse118I RCCGGY 1 cut(s) 1587
Bse1I ACTGG 7 cut(s) 126, 198, 378, 613, 1382, 1561, 1682
Bse21I CCTNAGG 1 cut(s) 1313
Bse8I GATNNNNATC 3 cut(s) 1236, 1488, 1510
BseBI CCWGG 3 cut(s) 81, 409, 1830
BseCI ATCGAT 2 cut(s) 508, 574
BseDI CCNNGG 6 cut(s) 244, 563, 913, 1265, 1671, 1729
BseGI GGATG 5 cut(s) 459, 648, 1158, 1348, 1443
BseJI GATNNNNATC 3 cut(s) 1236, 1488, 1510
BseLI CCNNNNNNNGG 4 cut(s) 250, 608, 1677, 1797
BseMII CTCAG 2 cut(s) 1367, 1787
BseNI ACTGG 7 cut(s) 126, 198, 378, 613, 1382, 1561, 1682
BseRI GAGGAG 1 cut(s) 99
BseX3I CGGCCG 1 cut(s) 848
Bsh1236I CGCG 1 cut(s) 896
Bsh1285I CGRYCG 3 cut(s) 432, 851, 1435
BshFI GGCC 7 cut(s) 115, 341, 850, 1381, 1560, 1670, 1820
BshVI ATCGAT 2 cut(s) 508, 574
BsiEI CGRYCG 3 cut(s) 432, 851, 1435
BsiHKAI GWGCWC 1 cut(s) 1814
BsiHKCI CYCGRG 1 cut(s) 913
BsiSI CCGG 3 cut(s) 914, 1588, 1837
BsiWI CGTACG 1 cut(s) 1389
BslFI GGGAC 1 cut(s) 957
BslI CCNNNNNNNGG 4 cut(s) 250, 608, 1677, 1797
BsmAI GTCTC 3 cut(s) 698, 1416, 1696
BsmFI GGGAC 1 cut(s) 957
BsnI GGCC 7 cut(s) 115, 341, 850, 1381, 1560, 1670, 1820
Bso31I GGTCTC 1 cut(s) 1416
BsoBI CYCGRG 1 cut(s) 913
Bsp119I TTCGAA 1 cut(s) 1845
Bsp1286I GDGCHC 1 cut(s) 1814
Bsp1407I TGTACA 2 cut(s) 516, 1066
BspACI CCGC 2 cut(s) 896, 1479
BspANI GGCC 7 cut(s) 115, 341, 850, 1381, 1560, 1670, 1820
BspCNI CTCAG 2 cut(s) 1368, 1788
BspDI ATCGAT 2 cut(s) 508, 574
BspFNI CGCG 1 cut(s) 896
BspHI TCATGA 1 cut(s) 1302
BspLI GGNNCC 1 cut(s) 198
BspMI ACCTGC 2 cut(s) 81, 1858
BspPI GGATC 3 cut(s) 50, 132, 1513
BspQI GCTCTTC 1 cut(s) 1569
BspT104I TTCGAA 1 cut(s) 1845
BspTI CTTAAG 1 cut(s) 1550
BspTNI GGTCTC 1 cut(s) 1416
BsrFI RCCGGY 1 cut(s) 1587
BsrGI TGTACA 2 cut(s) 516, 1066
BsrI ACTGG 7 cut(s) 126, 198, 378, 613, 1382, 1561, 1682
BssAI RCCGGY 1 cut(s) 1587
BssECI CCNNGG 6 cut(s) 244, 563, 913, 1265, 1671, 1729
BssNI GRCGYC 1 cut(s) 899
BssT1I CCWWGG 3 cut(s) 244, 1265, 1671
Bst2UI CCWGG 3 cut(s) 81, 409, 1830
Bst4CI ACNGT 5 cut(s) 150, 767, 776, 1321, 1630
Bst6I CTCTTC 4 cut(s) 885, 1140, 1304, 1569
BstACI GRCGYC 1 cut(s) 899
BstAFI CTTAAG 1 cut(s) 1550
BstAUI TGTACA 2 cut(s) 516, 1066
BstBAI YACGTR 1 cut(s) 362
BstBI TTCGAA 1 cut(s) 1845
BstDEI CTNAG 3 cut(s) 1313, 1376, 1796
BstDSI CCRYGG 1 cut(s) 1729
BstENI CCTNNNNNAGG 1 cut(s) 1795
BstF5I GGATG 5 cut(s) 459, 648, 1158, 1348, 1443
BstFNI CGCG 1 cut(s) 896
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 2 cut(s) 426, 1860
BstMAI GTCTC 3 cut(s) 698, 1416, 1696
BstMCI CGRYCG 3 cut(s) 432, 851, 1435
BstMWI GCNNNNNNNGC 2 cut(s) 966, 1809
BstNI CCWGG 3 cut(s) 81, 409, 1830
BstNSI RCATGY 3 cut(s) 11, 485, 1549
BstSCI CCNGG 5 cut(s) 79, 407, 912, 913, 1828
BstUI CGCG 1 cut(s) 896
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BstZI CGGCCG 1 cut(s) 848
Bsu15I ATCGAT 2 cut(s) 508, 574
Bsu36I CCTNAGG 1 cut(s) 1313
BsuRI GGCC 7 cut(s) 115, 341, 850, 1381, 1560, 1670, 1820
BsuTUI ATCGAT 2 cut(s) 508, 574
BtgI CCRYGG 1 cut(s) 1729
BtsCI GGATG 5 cut(s) 459, 648, 1158, 1348, 1443
BtsIMutI CAGTG 3 cut(s) 191, 781, 1389
BveI ACCTGC 2 cut(s) 81, 1858
CciI TCATGA 1 cut(s) 1302
CfoI GCGC 2 cut(s) 426, 1860
Cfr10I RCCGGY 1 cut(s) 1587
Cfr13I GGNCC 2 cut(s) 196, 618
Cfr9I CCCGGG 1 cut(s) 913
ClaI ATCGAT 2 cut(s) 508, 574
CseI GACGC 1 cut(s) 888
CspCI CAANNNNNGTGG 2 cut(s) 343, 378
CviAII CATG 9 cut(s) 8, 482, 662, 711, 742, 1303, 1496, 1546, 1723
DdeI CTNAG 3 cut(s) 1313, 1376, 1796
DraI TTTAAA 1 cut(s) 109
EaeI YGGCCR 2 cut(s) 848, 1668
EagI CGGCCG 1 cut(s) 848
Eam1104I CTCTTC 4 cut(s) 885, 1140, 1304, 1569
EarI CTCTTC 4 cut(s) 885, 1140, 1304, 1569
Ecl136II GAGCTC 1 cut(s) 1812
EclXI CGGCCG 1 cut(s) 848
Eco130I CCWWGG 3 cut(s) 244, 1265, 1671
Eco24I GRGCYC 1 cut(s) 1814
Eco31I GGTCTC 1 cut(s) 1416
Eco47I GGWCC 2 cut(s) 196, 618
Eco52I CGGCCG 1 cut(s) 848
Eco53kI GAGCTC 1 cut(s) 1812
Eco57I CTGAAG 1 cut(s) 284
Eco72I CACGTG 1 cut(s) 362
Eco81I CCTNAGG 1 cut(s) 1313
Eco88I CYCGRG 1 cut(s) 913
EcoICRI GAGCTC 1 cut(s) 1812
EcoNI CCTNNNNNAGG 1 cut(s) 1795
EcoRII CCWGG 3 cut(s) 79, 407, 1828
EcoT14I CCWWGG 3 cut(s) 244, 1265, 1671
EcoT22I ATGCAT 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 1814
ErhI CCWWGG 3 cut(s) 244, 1265, 1671
FaeI CATG 9 cut(s) 11, 485, 665, 714, 745, 1306, 1499, 1549, 1726
FaqI GGGAC 1 cut(s) 957
FatI CATG 9 cut(s) 7, 481, 661, 710, 741, 1302, 1495, 1545, 1722
FauNDI CATATG 1 cut(s) 991
FbaI TGATCA 1 cut(s) 1483
Fnu4HI GCNGC 1 cut(s) 897
FokI GGATG 5 cut(s) 446, 635, 1165, 1355, 1450
FriOI GRGCYC 1 cut(s) 1814
Fsp4HI GCNGC 1 cut(s) 897
FspBI CTAG 2 cut(s) 96, 806
GlaI GCGC 2 cut(s) 425, 1859
GluI GCNGC 1 cut(s) 897
GsuI CTGGAG 2 cut(s) 102, 1262
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 7 cut(s) 115, 341, 850, 1381, 1560, 1670, 1820
HapII CCGG 3 cut(s) 914, 1588, 1837
HgaI GACGC 1 cut(s) 888
HhaI GCGC 2 cut(s) 426, 1860
Hin1I GRCGYC 1 cut(s) 899
Hin1II CATG 9 cut(s) 11, 485, 665, 714, 745, 1306, 1499, 1549, 1726
Hin6I GCGC 2 cut(s) 424, 1858
HinP1I GCGC 2 cut(s) 424, 1858
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 1653
HinfI GANTC 6 cut(s) 23, 386, 875, 1246, 1489, 1833
HpaII CCGG 3 cut(s) 914, 1588, 1837
HphI GGTGA 3 cut(s) 84, 1168, 1493
Hpy166II GTNNAC 3 cut(s) 27, 355, 1272
Hpy188I TCNGA 8 cut(s) 564, 723, 863, 978, 1102, 1377, 1437, 1488
Hpy8I GTNNAC 3 cut(s) 27, 355, 1272
Hpy99I CGWCG 3 cut(s) 904, 1397, 1606
HpyAV CCTTC 4 cut(s) 554, 608, 1436, 1448
HpyCH4III ACNGT 5 cut(s) 150, 767, 776, 1321, 1630
HpyCH4IV ACGT 5 cut(s) 361, 1073, 1203, 1388, 1770
HpyCH4V TGCA 8 cut(s) 7, 76, 137, 316, 656, 1013, 1081, 1701
HpyF10VI GCNNNNNNNGC 2 cut(s) 966, 1809
HpyF3I CTNAG 3 cut(s) 1313, 1376, 1796
HpySE526I ACGT 5 cut(s) 361, 1073, 1203, 1388, 1770
Hsp92I GRCGYC 1 cut(s) 899
Hsp92II CATG 9 cut(s) 11, 485, 665, 714, 745, 1306, 1499, 1549, 1726
HspAI GCGC 2 cut(s) 424, 1858
Ksp22I TGATCA 1 cut(s) 1483
LguI GCTCTTC 1 cut(s) 1569
LmnI GCTCC 2 cut(s) 86, 671
LweI GCATC 1 cut(s) 1222
MaeI CTAG 2 cut(s) 96, 806
MaeII ACGT 5 cut(s) 361, 1073, 1203, 1388, 1770
MaeIII GTNAC 6 cut(s) 189, 271, 433, 767, 1384, 1771
MfeI CAATTG 1 cut(s) 1082
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 1 cut(s) 1814
MlsI TGGCCA 1 cut(s) 1670
MluCI AATT 9 cut(s) 35, 104, 168, 330, 812, 952, 1014, 1082, 1864
MluNI TGGCCA 1 cut(s) 1670
MlyI GAGTC 2 cut(s) 32, 1842
MmeI TCCRAC 2 cut(s) 885, 1080
MnlI CCTC 8 cut(s) 77, 558, 598, 1096, 1371, 1537, 1791, 1831
Mox20I TGGCCA 1 cut(s) 1670
Mph1103I ATGCAT 1 cut(s) 9
MroXI GAANNNNTTC 3 cut(s) 1250, 1403, 1578
MscI TGGCCA 1 cut(s) 1670
MseI TTAA 8 cut(s) 38, 69, 108, 345, 956, 1122, 1551, 1632
MslI CAYNNNNRTG 2 cut(s) 994, 1500
Msp20I TGGCCA 1 cut(s) 1670
MspCI CTTAAG 1 cut(s) 1550
MspI CCGG 3 cut(s) 914, 1588, 1837
MspR9I CCNGG 5 cut(s) 81, 409, 914, 915, 1830
MunI CAATTG 1 cut(s) 1082
MvaI CCWGG 3 cut(s) 81, 409, 1830
MvnI CGCG 1 cut(s) 896
MwoI GCNNNNNNNGC 2 cut(s) 966, 1809
NciI CCSGG 2 cut(s) 914, 915
NdeI CATATG 1 cut(s) 991
NlaIII CATG 9 cut(s) 11, 485, 665, 714, 745, 1306, 1499, 1549, 1726
NlaIV GGNNCC 1 cut(s) 198
NmuCI GTSAC 3 cut(s) 189, 767, 1384
NsiI ATGCAT 1 cut(s) 9
NspI RCATGY 3 cut(s) 11, 485, 1549
NspV TTCGAA 1 cut(s) 1845
PagI TCATGA 1 cut(s) 1302
PciI ACATGT 1 cut(s) 481
PciSI GCTCTTC 1 cut(s) 1569
PcsI WCGNNNNNNNCGW 2 cut(s) 425, 1712
PdmI GAANNNNTTC 3 cut(s) 1250, 1403, 1578
PfeI GAWTC 4 cut(s) 386, 875, 1246, 1489
Pfl23II CGTACG 1 cut(s) 1389
PflMI CCANNNNNTGG 2 cut(s) 608, 1677
PkrI GCNGC 1 cut(s) 898
Ple19I CGATCG 2 cut(s) 432, 1435
PleI GAGTC 2 cut(s) 31, 1841
PmaCI CACGTG 1 cut(s) 362
PmlI CACGTG 1 cut(s) 362
PpsI GAGTC 2 cut(s) 31, 1841
Ppu21I YACGTR 1 cut(s) 362
PscI ACATGT 1 cut(s) 481
Psp124BI GAGCTC 1 cut(s) 1814
Psp1406I AACGTT 1 cut(s) 1770
Psp6I CCWGG 3 cut(s) 79, 407, 1828
PspCI CACGTG 1 cut(s) 362
PspGI CCWGG 3 cut(s) 79, 407, 1828
PspLI CGTACG 1 cut(s) 1389
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 2 cut(s) 196, 618
PsuI RGATCY 1 cut(s) 298
PvuI CGATCG 2 cut(s) 432, 1435
RseI CAYNNNNRTG 2 cut(s) 994, 1500
SacI GAGCTC 1 cut(s) 1814
SapI GCTCTTC 1 cut(s) 1569
SaqAI TTAA 8 cut(s) 38, 69, 108, 345, 956, 1122, 1551, 1632
SatI GCNGC 1 cut(s) 897
Sau96I GGNCC 2 cut(s) 196, 618
ScaI AGTACT 1 cut(s) 757
SchI GAGTC 2 cut(s) 32, 1842
ScrFI CCNGG 5 cut(s) 81, 409, 914, 915, 1830
SduI GDGCHC 1 cut(s) 1814
SfaNI GCATC 1 cut(s) 1222
SfuI TTCGAA 1 cut(s) 1845
SinI GGWCC 2 cut(s) 196, 618
SmaI CCCGGG 1 cut(s) 915
SmiMI CAYNNNNRTG 2 cut(s) 994, 1500
SmlI CTYRAG 2 cut(s) 751, 1550
SmoI CTYRAG 2 cut(s) 751, 1550
Sse9I AATT 9 cut(s) 35, 104, 168, 330, 812, 952, 1014, 1082, 1864
SsiI CCGC 2 cut(s) 896, 1479
SspI AATATT 3 cut(s) 162, 793, 867
SspMI CTAG 2 cut(s) 96, 806
SstI GAGCTC 1 cut(s) 1814
StyD4I CCNGG 5 cut(s) 79, 407, 912, 913, 1828
StyI CCWWGG 3 cut(s) 244, 1265, 1671
TaaI ACNGT 5 cut(s) 150, 767, 776, 1321, 1630
TaiI ACGT 5 cut(s) 364, 1076, 1206, 1391, 1773
TasI AATT 9 cut(s) 35, 104, 168, 330, 812, 952, 1014, 1082, 1864
TatI WGTACW 7 cut(s) 98, 478, 516, 664, 755, 986, 1066
TauI GCSGC 1 cut(s) 899
TfiI GAWTC 4 cut(s) 386, 875, 1246, 1489
Tru1I TTAA 8 cut(s) 38, 69, 108, 345, 956, 1122, 1551, 1632
Tru9I TTAA 8 cut(s) 38, 69, 108, 345, 956, 1122, 1551, 1632
TscAI CASTG 3 cut(s) 198, 781, 1389
TseFI GTSAC 3 cut(s) 189, 767, 1384
Tsp45I GTSAC 3 cut(s) 189, 767, 1384
TspGWI ACGGA 2 cut(s) 1466, 1590
TspMI CCCGGG 1 cut(s) 913
TspRI CASTG 3 cut(s) 198, 781, 1389
Van91I CCANNNNNTGG 2 cut(s) 608, 1677
Vha464I CTTAAG 1 cut(s) 1550
VpaK11BI GGWCC 2 cut(s) 196, 618
XagI CCTNNNNNAGG 1 cut(s) 1795
XapI RAATTY 4 cut(s) 104, 330, 812, 952
XbaI TCTAGA 1 cut(s) 805
XceI RCATGY 3 cut(s) 11, 485, 1549
XmaI CCCGGG 1 cut(s) 913
XmnI GAANNNNTTC 3 cut(s) 1250, 1403, 1578
XspI CTAG 2 cut(s) 96, 806
ZrmI AGTACT 1 cut(s) 757
Zsp2I ATGCAT 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.