FvH4_4g22401

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000147
Physical Location & Seq
Reverse (-)
25242097 .. 25243903
1807 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22401.t1

Sequence Viewer

Length: 777 bp
ATGAGGAGTCCAGCAGAACTCTTCTTTCTTGAAGTCGAAAGAAATTATGTGGCTTCCTCCTGCAACATTAACATGACTTCCTGCTTCTTCCCCAGTGAATTGAGGCTTCTCAATCTGAATCTTGAAGCCGCCGGCGAAAGTCGGAGATGGCCACCTTGGCCGGACTGGCTTTCTCCTTCGATTCCTACTGCCCTTGATCACCATATGCCTCTTTTGGAGGAACCCATGGTCACAACAACAACCCATGTATTCGAAAACCCCATTTCATCTTGCCACTCTCAAACCAATCTTTTAGCTTCACCAGGATCCAATCCCTTATCCCACCTTAAATTGAACGCCTACCACGAGATGGGCTCAATCACTGCATGTTTGATCGGAGCAGTTATGGAAAAATTTGGTGGATCCGACAATTTCCAAGGGTATCGGGCATGCAACAAAGGTACCTCTACCACTAATCAGATATATCTGATTTCTCAGGATAAACAAATGGCCCTGTGGAATGAACTATCAATGCTTAGCCCGGATAATAATACTTGCTGGGTGTTAATGGGAGACTTCAACATAACTGTGTCCCCTGAGGAAAAACTGGGGGGTATTGGAGGTGTAACAAACTTCATGTTAAATTTTTGTGATTTCTTGAATAACAATGGTTTAGTGTCTATGGCTGCTGCAGGTTTGCCGTTCACCTGGACAAACAAGCATGTAGATGATATTGTGATCTTCGAAAGGCTAGACAGAGTTATTGTGAATTCTAGTTTCTTGAACTATTTTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

259

Amino Acids

28.81

Weight (kDa)

4.97

Isoelectric Point (pI)

50.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000147)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10354 FvH4_1g17131 FvH4_2g11611 FvH4_2g33851 FvH4_3g32112 FvH4_3g35052 FvH4_3g44611 FvH4_4g05921 FvH4_4g06411 FvH4_4g06792 FvH4_4g10365 FvH4_4g21422 FvH4_4g22401 FvH4_4g30261 FvH4_5g08843 FvH4_5g14731 FvH4_5g24781 FvH4_5g26231 FvH4_5g31282 FvH4_6g23371 FvH4_6g38511 FvH4_7g01171 FvH4_7g03341 FvH4_7g06171 FvH4_7g32472
prunus_persica Prupe.2G036900_v2.0.a1
pyrus_communis pycom02g19900 pycom03g01520 pycom05g10510 pycom16g23820
rosa_chinensis RchiOBHm_Chr1g0316031 RchiOBHm_Chr1g0342451 RchiOBHm_Chr1g0344541 RchiOBHm_Chr1g0364721 RchiOBHm_Chr2g0133541 RchiOBHm_Chr2g0134511 RchiOBHm_Chr2g0145891 RchiOBHm_Chr2g0163241 RchiOBHm_Chr5g0027201 RchiOBHm_Chr5g0027881 RchiOBHm_Chr5g0047471 RchiOBHm_Chr7g0210901
rosa_laevigata RLG00000011116 RLG00000017964 RLG00000018128 RLG00000018387 RLG00000018829 RLG00000019353 RLG00000028973 RLG00000035870 RLG00000036629
rosa_multiflora Rmu_co8126340.1_g000001 Rmu_co8216750.1_g000001 Rmu_co8222072.1_g000001 Rmu_co8332453.1_g000001 Rmu_co8349913.1_g000001 Rmu_co8405337.1_g000001 Rmu_sc0000041.1_g000012 Rmu_sc0000166.1_g000029 Rmu_sc0000251.1_g000019 Rmu_sc0000271.1_g000028 Rmu_sc0000509.1_g000036 Rmu_sc0000543.1_g000048 Rmu_sc0000808.1_g000003 Rmu_sc0000847.1_g000040 Rmu_sc0000972.1_g000020 Rmu_sc0001260.1_g000001 Rmu_sc0001464.1_g000026 Rmu_sc0001616.1_g000003 Rmu_sc0001669.1_g000001 Rmu_sc0002005.1_g000001 Rmu_sc0002014.1_g000010 Rmu_sc0002082.1_g000033 Rmu_sc0002302.1_g000024 Rmu_sc0002531.1_g000045 Rmu_sc0003276.1_g000002 Rmu_sc0003737.1_g000007 Rmu_sc0003832.1_g000022 Rmu_sc0004080.1_g000011 Rmu_sc0004487.1_g000009 Rmu_sc0004988.1_g000018 Rmu_sc0004988.1_g000019 Rmu_sc0005254.1_g000002 Rmu_sc0005733.1_g000004 Rmu_sc0006413.1_g000006 Rmu_sc0007121.1_g000013 Rmu_sc0007869.1_g000006 Rmu_sc0008035.1_g000015 Rmu_sc0008279.1_g000017 Rmu_sc0008351.1_g000014 Rmu_sc0008957.1_g000007 Rmu_sc0009012.1_g000007 Rmu_sc0010556.1_g000009 Rmu_sc0012889.1_g000003 Rmu_sc0019808.1_g000004 Rmu_sc0028113.1_g000001 Rmu_sc0031202.1_g000001 Rmu_sc0034436.1_g000001 Rmu_sc0034445.1_g000001 Rmu_sc0040044.1_g000001 Rmu_ssc0000062.1_g000032 Rmu_ssc0000372.1_g000018
rosa_roxburghii Rroxscaffold_1G00001520 Rroxscaffold_1G00039990 Rroxscaffold_1G00046370 Rroxscaffold_2G00110140 Rroxscaffold_2G00131420 Rroxscaffold_3G00222390 Rroxscaffold_5G00336040 Rroxscaffold_5G00338000 Rroxscaffold_5G00344030 Rroxscaffold_5G00363460 Rroxscaffold_6G00402000 Rroxscaffold_7G00194940
rosa_rugosa Rorug01G0106500 Rorug01G0111300 Rorug01G0247000 Rorug02G0318100.1 Rorug02G0318200 Rorug02G0459500 Rorug03G0108100 Rorug03G0200500 Rorug03G0232500 Rorug03G0240600 Rorug04G0105300 Rorug04G0256600 Rorug06G0079300 Rorug06G0082700 Rorug06G0095600 Rorug06G0179000 Rorug07G0113500 Rorug07G0271100
rosa_samantha Rh1AG087200 Rh1CG085300 Rh1DG013200 Rh1DG091700 Rh1DG091800 Rh1DG338300 Rh2AG375400 Rh2AG407000 Rh2AG513500 Rh2BG277700 Rh2BG371200 Rh2BG376100 Rh2BG580500 Rh2CG354300 Rh2DG200100 Rh2DG388000 Rh2DG393500 Rh2DG426400 Rh3CG304800 Rh4AG044600 Rh4AG220500 Rh4BG379300 Rh4CG007100 Rh4CG030300 Rh4CG038000 Rh4CG114900 Rh4CG393700 Rh5AG124900 Rh5AG342300 Rh5BG414400 Rh5CG263800 Rh5CG439000 Rh5DG231100 Rh5DG566900 Rh6AG138600 Rh6AG200000 Rh6AG253100 Rh6AG390900 Rh6AG441600 Rh6BG194700 Rh6BG225800 Rh6BG256100 Rh6CG231700 Rh6CG231900 Rh6CG312200 Rh6CG453700 Rh6DG219400 Rh7AG357700 Rh7AG442000 Rh7CG425700 Rh7CG474600 Rh7CG494100 Rh7DG253900 Rh7DG294700 Rh7DG401600 Rh7DG461300
rosa_wichuraiana Rw1G001190 Rw2G047020 Rw4G003490 Rw5G039690 Rw6G010450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 662
Acc65I GGTACC 1 cut(s) 440
AccB1I GGYRCC 1 cut(s) 440
AccB7I CCANNNNNTGG 1 cut(s) 349
AciI CCGC 1 cut(s) 129
AclWI GGATC 4 cut(s) 300, 313, 396, 409
AcoI YGGCCR 2 cut(s) 149, 158
AcsI RAATTY 3 cut(s) 392, 622, 748
AfaI GTAC 1 cut(s) 442
AfiI CCNNNNNNNGG 1 cut(s) 349
AgsI TTSAA 6 cut(s) 32, 125, 334, 559, 640, 763
AjnI CCWGG 2 cut(s) 301, 686
AluBI AGCT 1 cut(s) 296
AluI AGCT 1 cut(s) 296
Alw26I GTCTC 1 cut(s) 546
AlwI GGATC 4 cut(s) 300, 313, 396, 409
AoxI GGCC 3 cut(s) 149, 158, 489
ApeKI GCWGC 2 cut(s) 665, 668
ApoI RAATTY 3 cut(s) 392, 622, 748
Asp718I GGTACC 1 cut(s) 440
AspS9I GGNCC 1 cut(s) 490
AsuC2I CCSGG 1 cut(s) 521
AsuHPI GGTGA 3 cut(s) 191, 291, 676
AsuII TTCGAA 2 cut(s) 252, 723
AxyI CCTNAGG 1 cut(s) 576
BaeI ACNNNNGTAYC 2 cut(s) 424, 457
BalI TGGCCA 1 cut(s) 151
BamHI GGATCC 2 cut(s) 305, 401
BanI GGYRCC 1 cut(s) 440
BanII GRGCYC 1 cut(s) 356
BauI CACGAG 1 cut(s) 344
BbvI GCAGC 2 cut(s) 652, 655
BccI CCATC 2 cut(s) 141, 343
BceAI ACGGC 1 cut(s) 664
BciT130I CCWGG 2 cut(s) 303, 688
BclI TGATCA 1 cut(s) 196
BcnI CCSGG 1 cut(s) 521
BcoDI GTCTC 1 cut(s) 546
BfaI CTAG 2 cut(s) 731, 753
BfmI CTRYAG 1 cut(s) 669
BfuAI ACCTGC 1 cut(s) 662
BglI GCCNNNNNGGC 2 cut(s) 157, 166
BisI GCNGC 3 cut(s) 129, 666, 669
BlpI GCTNAGC 1 cut(s) 515
BlsI GCNGC 3 cut(s) 130, 667, 670
Bme1390I CCNGG 3 cut(s) 303, 521, 688
BmgT120I GGNCC 1 cut(s) 490
BmiI GGNNCC 4 cut(s) 222, 307, 403, 442
BmrFI CCNGG 3 cut(s) 303, 521, 688
BmrI ACTGGG 2 cut(s) 87, 596
BmuI ACTGGG 2 cut(s) 87, 596
BplI GAGNNNNNCTC 2 cut(s) 338, 370
Bpu1102I GCTNAGC 1 cut(s) 515
Bpu14I TTCGAA 2 cut(s) 252, 723
BpuMI CCSGG 1 cut(s) 521
BsaJI CCNNGG 3 cut(s) 155, 225, 415
Bsc4I CCNNNNNNNGG 1 cut(s) 349
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 3 cut(s) 93, 170, 591
Bse21I CCTNAGG 1 cut(s) 576
BseBI CCWGG 2 cut(s) 303, 688
BseDI CCNNGG 3 cut(s) 155, 225, 415
BseLI CCNNNNNNNGG 1 cut(s) 349
BseMII CTCAG 2 cut(s) 488, 567
BseNI ACTGG 3 cut(s) 93, 170, 591
BseRI GAGGAG 1 cut(s) 19
BseXI GCAGC 2 cut(s) 652, 655
BseYI CCCAGC 1 cut(s) 537
BshFI GGCC 3 cut(s) 151, 160, 491
BshNI GGYRCC 1 cut(s) 440
BsiSI CCGG 3 cut(s) 132, 161, 521
BslFI GGGAC 1 cut(s) 556
BslI CCNNNNNNNGG 1 cut(s) 349
BsmAI GTCTC 1 cut(s) 546
BsmFI GGGAC 1 cut(s) 556
BsnI GGCC 3 cut(s) 151, 160, 491
Bsp119I TTCGAA 2 cut(s) 252, 723
Bsp1286I GDGCHC 1 cut(s) 356
Bsp143I GATC 5 cut(s) 196, 305, 372, 401, 717
Bsp1720I GCTNAGC 1 cut(s) 515
Bsp19I CCATGG 1 cut(s) 225
BspACI CCGC 1 cut(s) 129
BspANI GGCC 3 cut(s) 151, 160, 491
BspCNI CTCAG 2 cut(s) 487, 568
BspLI GGNNCC 4 cut(s) 222, 307, 403, 442
BspMAI CTGCAG 1 cut(s) 673
BspMI ACCTGC 1 cut(s) 662
BspPI GGATC 4 cut(s) 300, 313, 396, 409
BspT104I TTCGAA 2 cut(s) 252, 723
BspT107I GGYRCC 1 cut(s) 440
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 3 cut(s) 93, 170, 591
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 3 cut(s) 155, 225, 415
BssMI GATC 5 cut(s) 196, 305, 372, 401, 717
BssSI CACGAG 1 cut(s) 344
BssT1I CCWWGG 3 cut(s) 155, 225, 415
Bst2BI CACGAG 1 cut(s) 344
Bst2UI CCWGG 2 cut(s) 303, 688
Bst4CI ACNGT 1 cut(s) 568
Bst6I CTCTTC 1 cut(s) 26
BstBI TTCGAA 2 cut(s) 252, 723
BstC8I GCNNGC 2 cut(s) 133, 430
BstDEI CTNAG 3 cut(s) 474, 515, 576
BstDSI CCRYGG 1 cut(s) 225
BstKTI GATC 5 cut(s) 199, 308, 375, 404, 720
BstMAI GTCTC 1 cut(s) 546
BstMBI GATC 5 cut(s) 196, 305, 372, 401, 717
BstMWI GCNNNNNNNGC 2 cut(s) 157, 166
BstNI CCWGG 2 cut(s) 303, 688
BstNSI RCATGY 3 cut(s) 369, 432, 704
BstSCI CCNGG 3 cut(s) 301, 519, 686
BstSFI CTRYAG 1 cut(s) 669
BstV1I GCAGC 2 cut(s) 652, 655
BstX2I RGATCY 2 cut(s) 305, 401
BstYI RGATCY 2 cut(s) 305, 401
Bsu36I CCTNAGG 1 cut(s) 576
BsuRI GGCC 3 cut(s) 151, 160, 491
BtgI CCRYGG 1 cut(s) 225
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 2 cut(s) 100, 360
BveI ACCTGC 1 cut(s) 662
Cac8I GCNNGC 2 cut(s) 133, 430
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 490
Csp6I GTAC 1 cut(s) 441
CviAII CATG 7 cut(s) 73, 226, 245, 366, 429, 616, 701
CviQI GTAC 1 cut(s) 441
DdeI CTNAG 3 cut(s) 474, 515, 576
DpnI GATC 5 cut(s) 198, 307, 374, 403, 719
DpnII GATC 5 cut(s) 196, 305, 372, 401, 717
EaeI YGGCCR 2 cut(s) 149, 158
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Eco130I CCWWGG 3 cut(s) 155, 225, 415
Eco24I GRGCYC 1 cut(s) 356
Eco81I CCTNAGG 1 cut(s) 576
EcoRI GAATTC 1 cut(s) 748
EcoRII CCWGG 2 cut(s) 301, 686
EcoT14I CCWWGG 3 cut(s) 155, 225, 415
EcoT38I GRGCYC 1 cut(s) 356
ErhI CCWWGG 3 cut(s) 155, 225, 415
FaeI CATG 7 cut(s) 76, 229, 248, 369, 432, 619, 704
FaqI GGGAC 1 cut(s) 556
FatI CATG 7 cut(s) 72, 225, 244, 365, 428, 615, 700
FauNDI CATATG 1 cut(s) 204
FbaI TGATCA 1 cut(s) 196
Fnu4HI GCNGC 3 cut(s) 129, 666, 669
FriOI GRGCYC 1 cut(s) 356
Fsp4HI GCNGC 3 cut(s) 129, 666, 669
FspBI CTAG 2 cut(s) 731, 753
GluI GCNGC 3 cut(s) 129, 666, 669
GsaI CCCAGC 1 cut(s) 541
HaeIII GGCC 3 cut(s) 151, 160, 491
HapII CCGG 3 cut(s) 132, 161, 521
Hin1II CATG 7 cut(s) 76, 229, 248, 369, 432, 619, 704
HinfI GANTC 3 cut(s) 7, 118, 181
HpaII CCGG 3 cut(s) 132, 161, 521
HphI GGTGA 3 cut(s) 191, 291, 676
Hpy166II GTNNAC 1 cut(s) 684
Hpy188I TCNGA 6 cut(s) 117, 144, 377, 406, 459, 468
Hpy188III TCNNGA 5 cut(s) 29, 122, 476, 637, 760
Hpy8I GTNNAC 1 cut(s) 684
HpyAV CCTTC 1 cut(s) 186
HpyCH4III ACNGT 1 cut(s) 568
HpyCH4V TGCA 4 cut(s) 63, 365, 432, 671
HpyF10VI GCNNNNNNNGC 2 cut(s) 157, 166
HpyF3I CTNAG 3 cut(s) 474, 515, 576
Hsp92II CATG 7 cut(s) 76, 229, 248, 369, 432, 619, 704
KpnI GGTACC 1 cut(s) 444
KroI GCCGGC 1 cut(s) 131
KroNI GCCGGC 1 cut(s) 133
Ksp22I TGATCA 1 cut(s) 196
Kzo9I GATC 5 cut(s) 196, 305, 372, 401, 717
LmnI GCTCC 1 cut(s) 377
Lsp1109I GCAGC 2 cut(s) 652, 655
MaeI CTAG 2 cut(s) 731, 753
MaeIII GTNAC 2 cut(s) 229, 604
MalI GATC 5 cut(s) 198, 307, 374, 403, 719
MboI GATC 5 cut(s) 196, 305, 372, 401, 717
MboII GAAGA 3 cut(s) 13, 79, 712
MflI RGATCY 2 cut(s) 305, 401
MhlI GDGCHC 1 cut(s) 356
MlsI TGGCCA 1 cut(s) 151
MluCI AATT 7 cut(s) 43, 98, 329, 392, 409, 622, 748
MluNI TGGCCA 1 cut(s) 151
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 2 cut(s) 122, 429
MnlI CCTC 7 cut(s) 67, 96, 211, 219, 454, 571, 593
Mox20I TGGCCA 1 cut(s) 151
MreI CGCCGGCG 1 cut(s) 131
MroNI GCCGGC 1 cut(s) 131
MscI TGGCCA 1 cut(s) 151
MseI TTAA 4 cut(s) 69, 327, 545, 620
MslI CAYNNNNRTG 3 cut(s) 71, 566, 705
Msp20I TGGCCA 1 cut(s) 151
MspI CCGG 3 cut(s) 132, 161, 521
MspR9I CCNGG 3 cut(s) 303, 521, 688
MvaI CCWGG 2 cut(s) 303, 688
MwoI GCNNNNNNNGC 2 cut(s) 157, 166
NaeI GCCGGC 1 cut(s) 133
NciI CCSGG 1 cut(s) 521
NcoI CCATGG 1 cut(s) 225
NdeI CATATG 1 cut(s) 204
NdeII GATC 5 cut(s) 196, 305, 372, 401, 717
NgoMIV GCCGGC 1 cut(s) 131
NlaIII CATG 7 cut(s) 76, 229, 248, 369, 432, 619, 704
NlaIV GGNNCC 4 cut(s) 222, 307, 403, 442
NmuCI GTSAC 1 cut(s) 229
NspI RCATGY 3 cut(s) 369, 432, 704
NspV TTCGAA 2 cut(s) 252, 723
PaeI GCATGC 1 cut(s) 432
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PdiI GCCGGC 1 cut(s) 133
PfeI GAWTC 2 cut(s) 118, 181
PflMI CCANNNNNTGG 1 cut(s) 349
PkrI GCNGC 3 cut(s) 130, 667, 670
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
Psp6I CCWGG 2 cut(s) 301, 686
PspFI CCCAGC 1 cut(s) 537
PspGI CCWGG 2 cut(s) 301, 686
PspN4I GGNNCC 4 cut(s) 222, 307, 403, 442
PspPI GGNCC 1 cut(s) 490
PstI CTGCAG 1 cut(s) 673
PsuI RGATCY 2 cut(s) 305, 401
RsaI GTAC 1 cut(s) 442
RsaNI GTAC 1 cut(s) 441
RseI CAYNNNNRTG 3 cut(s) 71, 566, 705
SaqAI TTAA 4 cut(s) 69, 327, 545, 620
SatI GCNGC 3 cut(s) 129, 666, 669
Sau3AI GATC 5 cut(s) 196, 305, 372, 401, 717
Sau96I GGNCC 1 cut(s) 490
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 3 cut(s) 303, 521, 688
SduI GDGCHC 1 cut(s) 356
SetI ASST 8 cut(s) 157, 298, 327, 442, 446, 604, 676, 689
SfcI CTRYAG 1 cut(s) 669
SfiI GGCCNNNNNGGCC 1 cut(s) 157
SfuI TTCGAA 2 cut(s) 252, 723
SgrAI CRCCGGYG 1 cut(s) 131
SmiMI CAYNNNNRTG 3 cut(s) 71, 566, 705
SphI GCATGC 1 cut(s) 432
Sse9I AATT 7 cut(s) 43, 98, 329, 392, 409, 622, 748
SsiI CCGC 1 cut(s) 129
SspMI CTAG 2 cut(s) 731, 753
StyD4I CCNGG 3 cut(s) 301, 519, 686
StyI CCWWGG 3 cut(s) 155, 225, 415
TaaI ACNGT 1 cut(s) 568
TaqI TCGA 4 cut(s) 36, 179, 252, 723
TasI AATT 7 cut(s) 43, 98, 329, 392, 409, 622, 748
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 2 cut(s) 118, 181
Tru1I TTAA 4 cut(s) 69, 327, 545, 620
Tru9I TTAA 4 cut(s) 69, 327, 545, 620
TscAI CASTG 2 cut(s) 100, 367
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 2 cut(s) 665, 668
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 3 cut(s) 255, 516, 604
TspRI CASTG 2 cut(s) 100, 367
Van91I CCANNNNNTGG 1 cut(s) 349
XapI RAATTY 3 cut(s) 392, 622, 748
XceI RCATGY 3 cut(s) 369, 432, 704
XspI CTAG 2 cut(s) 731, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.