FvH4_7g01171

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000147
Physical Location & Seq
Reverse (-)
1580771 .. 1585755
4985 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01171.t1

Sequence Viewer

Length: 4056 bp
ATGGAGGAGTCACATCGGGCACACGGCTCAACTTCTGTGTGGGATGGGATTTCTGACAGGGTGCCTCCTATACCCACTAGGCAACCATGGTGTATTGAAGGGCAGGGTGCTGGATGGCAGGATACAATGGAGGAGTCACTTCGGGCTCACGGCTCAACTTCTGTGTGGGATGGGATTTCTGACAGGGTGCCTCCTTTAAAGGAGAAGACCATATCACCAGCTCGATCAAAGCTTTCGCGCAATCCACTTGGGCATTACTATTGGGATCCGGATGCACATGGTGCACTGTTTCAAAATGGGAGTGTGACTAGGGCATTGGGTGGGCTGAGCTTGCATGATGGTCAATGGGCTGACCCAGAGTGTATACCACCATGGGCTTTCAAAAACAAGGCAGATTATTATGATGGGTTTGTTGGTGGATGCACAAAGCTCACTACTGTGCGACTACTTGAGATTGATCAGCCAGCTTTGATTGAAAAAGAGGAGATACTTGTGGTTCTAGAAAGCGGGTCTACAAAAAGAAAGAGGAAACCCAAGAAGACTATGGAGGAGGTAGAGGAAAACAAGAGCAATTCCAAACGCCCAAAGCTTACGAATGGACCTAGCCTTCGCCTCTCCTCTTCTGTGGGGCGAGGACGGGGTCGTGGACGGAGGGGCCGCGGTAGGGGGAGATCTACTTTTGTTTGCGGTGAATCACTATTGGGAACTGAATTTGTGACAATCTCTCAAGCAGTCGATGGTGCTGGTTTGGTTATTGATAGAGAGAGTTCTAAAGTGACAGGTTTAGGGAAGTCCTTGACAATCCAAACTCTAAGAGAGATAACTCGTTCCCATGATTTGAGTTTTGTTTTTCTCTCGGAAACTCACAAGAATGATAATTATGTGAATAAGGTGCGCAGACAACTGGGTTTCAGCAAAGGCTACCATGTTTCTCCTATAAACACAGCGGGCGGTTTGAGTCTATGGTGGAGACCAAACTACAAGGTTGAGGTAGTTGGTTACTCAAAATTTTTCATTGATACTTTGATTACTTCCCCTGGATCAGCTACTCCCTTTCGAGTTACTTGGATGTACAGCCCACCCTATGGTGAGGACAAGGCTGCATTTTTGGGAAAAGTGGGATCGAGTTTCACATGGGCTCGTAAAGAAGATGGAATTGTGGTTCTACAAGAGAGGTTGGACCGTAGTCTTTTAAATGAATGTTGGATTATGCACTGGCCCAATACCCACCTTACTCATTTGGCGAGGGTTGGGTCCGACCATAATCCTTTACTTCTATCTACGGAACTTCCTTGTGATAAAAGGAGTCACATCTTTCGGTTTGAAGCGGGTTGGGCTGATGAGAAGTTACAAGGTACTGATCCATATGGTTTTTCCTCTACCCAATTAGATCTCACTAACCGGTTGGGTACTCTTTGGCTGAGGGAGGAGAAATACTGGCGTCAAAGATCTAGAATCCGTTGGCTCTCGGCGGGTGATTCCAATACTTGTTTCTTCCACTTATCTACTCTTCATCGGAGACAAAGGAACCGGATACTTAAGATCCAGAATGATACTGATCAGTGGATTGTTGGGGAGAAGTTTATTAGATTGGAGTTTGAGAATCAATTCCAACAGACTTTTACCAGTAGTGGTTTAAGGGATTGGGGGGATGCTTTGAGGGGAGTTGCCCCTGTTATTACTGATGAGATGAATAATGATCTCGGCCGGCCTTTCACTTATGATGAAGTTAGAGACGCAGCTTTTCAGTTGGGAGCGTTGAAAGCTCCAGGGCCAGACGGATTTCCAGGCCTCTTCTATCATAAGTATTGGGGAATTGTTAATGAGATCATAAGGACTACTTCTTCTGATTTCCTTGTTGGGAAGGATAATATTATGCTAGCCCATGAGGTGTTTCATTATCTCAGGTTGAAGAAGGATGGGGAGAGCCATGAGTTCGGTTTAAAACTTGACATGAATAAAGCTTATGATAGGGTTGAGTGGGATTTTCTTGAAGCTACTCTTCGTAGGTTTGGTTTTGCTAATGGTTGGGTGAACCTAATTATGGCATGTGTGACTACAGTGTCCTTTTCTATCGTTCTTAATGGCTGTCTTGGGAGTACTTTCTGTCCGAGTAGAGGGTTGAGGCAAGGAGACCCTCTCTCTCCTTACTTATTTCTTTTGGTTAGTGAGGTTCTTTCTCTTCGTATTACCAAAGCTGTTGATGAGAGGAGTTTACTTACTGTGAGACTTGCCTGTAAGTGTCCCCCATTGTCCCACCTATGTTTTGCTGATGATTCACTATTTTTCATGAAGGCCACCCTCCTCAACTGTTCCAAGATATCTATGATATTCAAAGAGTATTGTTTGGCTTCTGATCAACTTATTAGTCTTGAAAAATCGAATATTTATTTTACTCCTAATACCTCTGATCAGGTGAAGCATCTTATGAGCTTGTTATTGGGTATCACTTGCACTGATCAACCGGGAGTATATCTGGGGCTCCCAACTTTTTGGGGAAGGTCAAAAAATGGGGCTTTAGCTTACATCAAAGAGAAGGTGGCGAACAAGATGGATGGGTGGAAAGAAAAGGTATTGACTCAAGCTGGCAAGGAGGTTCTTATCAAGTCTGTGGCTCTTGCTATTCCCTCCTTCCCCATGTCATGTTTCAGATTTCCTAAAGATATTTGTGATGATTTAAATGCTGCATTAGGGAATTTTTGGTGGGGGTCTAATGGGACTGGGAACAACATCCACTGGAAAAGTTGGCAATTTCTGGGTTTACCTAAAGAGAAAGGAGGAATGGGTTTTAGGGACCTGCATGAGTTTAATCTTGCATTGATTGCTAAGCAAGCTTGGAGACTAACCAATGATCCGAACTCTTTATGGGCTAGGATTGTTTCAGGAGTTATGGTAAACATATGGACTGACTGTTGGCTTCCGCCGACTGACTTAAACGATCCGGTTCGCCCAATCCATACAACTGGACTTATTCCGAGTCATGCTCCTATCATGGTTGAAGAGATTATTGATTGGGATACTCGATCTTGGCATCTAGGAAACATCAGTCATCTAATCCAGCAAGCAGACTTGAATCGTATTCTATCCATTCCTATTGGCAGGAGGGAGGACCCTGACCGTCGAATATGGCCATTAACTAAGCATGGTACTTTTACTGTCAAGTCAGCTTATCACTGGTACCACCATATGCAGTCAACTGCTCCTATCAGTAAATATGGGTCTTCCCACACCATTGATACTAGTTGCTGGAAGTTAATATGGAAACTGCCTACGCTCCCAAAGATTAGGCAATTCTTTTGGAAAGCCTTGAATGCATCTACTCCTACTTATCTTAGTCTCTATCGGAGAAAGATTATCAAATCTCTGTTGTGCCCTATTGACAAACAATCATTCACTACACTCGATACTTGGTTATTACAAGTGATTAAGAAATTCAGTAGCAAGAAGGTTCGTCAATGGGTTCTTACCTTGCTTGGTTGGGAGGCGGCTTCTGAGTTCACGACATCGACAAATCCTACTGCTGAATCTCCTATGTCTGAACAAATTTTGAACTGGATTCCACCTCCAATGGACATGATTGCTATCAATTGTGATGGGAGTTGGGATATTTCTGGATTGGGGGGCAAAGGTGTGGTGATTAGAAACCACCTGGGGTCATTTATGGGTGGTCTTGCTTCCCCGGTCCGCGCTAGCTCTGTAGAGGCAGTGGAAGCGCTAGCCATTCTTGCCAGCGTCAATCTCGCCGTTGACATGAATTTGAAAGCAGTCCAAGTTCAATCTGATTCACAATCAGTCATCTCAGATCTAAATTCTATTGCCACGTGCAATAATTGGAGAACTAATCTGATTGTTGAAGAAATCAAATGGAAGAGCAGGTTTTTTGATCGGGTTCACTGGAGGTGGATACCGCGAGAAGCTAATTCTGCTGCTCACACAGCAGCACAGCTTGGAAAACAGGCGTTGGGCCTCTGCATCTGGTCGGAGACTCCCCTACCGTCTCTCACTATGGTTTTGAGAAATGATGGGCTTCCATGCCCTCATGATGTTGTAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

1352

Amino Acids

152.49

Weight (kDa)

8.94

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 621 - 807 1.2e-15 Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_3 PF13456 1196 - 1315 1.4e-20 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000147)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10354 FvH4_1g17131 FvH4_2g11611 FvH4_2g33851 FvH4_3g32112 FvH4_3g35052 FvH4_3g44611 FvH4_4g05921 FvH4_4g06411 FvH4_4g06792 FvH4_4g10365 FvH4_4g21422 FvH4_4g22401 FvH4_4g30261 FvH4_5g08843 FvH4_5g14731 FvH4_5g24781 FvH4_5g26231 FvH4_5g31282 FvH4_6g23371 FvH4_6g38511 FvH4_7g01171 FvH4_7g03341 FvH4_7g06171 FvH4_7g32472
prunus_persica Prupe.2G036900_v2.0.a1
pyrus_communis pycom02g19900 pycom03g01520 pycom05g10510 pycom16g23820
rosa_chinensis RchiOBHm_Chr1g0316031 RchiOBHm_Chr1g0342451 RchiOBHm_Chr1g0344541 RchiOBHm_Chr1g0364721 RchiOBHm_Chr2g0133541 RchiOBHm_Chr2g0134511 RchiOBHm_Chr2g0145891 RchiOBHm_Chr2g0163241 RchiOBHm_Chr5g0027201 RchiOBHm_Chr5g0027881 RchiOBHm_Chr5g0047471 RchiOBHm_Chr7g0210901
rosa_laevigata RLG00000011116 RLG00000017964 RLG00000018128 RLG00000018387 RLG00000018829 RLG00000019353 RLG00000028973 RLG00000035870 RLG00000036629
rosa_multiflora Rmu_co8126340.1_g000001 Rmu_co8216750.1_g000001 Rmu_co8222072.1_g000001 Rmu_co8332453.1_g000001 Rmu_co8349913.1_g000001 Rmu_co8405337.1_g000001 Rmu_sc0000041.1_g000012 Rmu_sc0000166.1_g000029 Rmu_sc0000251.1_g000019 Rmu_sc0000271.1_g000028 Rmu_sc0000509.1_g000036 Rmu_sc0000543.1_g000048 Rmu_sc0000808.1_g000003 Rmu_sc0000847.1_g000040 Rmu_sc0000972.1_g000020 Rmu_sc0001260.1_g000001 Rmu_sc0001464.1_g000026 Rmu_sc0001616.1_g000003 Rmu_sc0001669.1_g000001 Rmu_sc0002005.1_g000001 Rmu_sc0002014.1_g000010 Rmu_sc0002082.1_g000033 Rmu_sc0002302.1_g000024 Rmu_sc0002531.1_g000045 Rmu_sc0003276.1_g000002 Rmu_sc0003737.1_g000007 Rmu_sc0003832.1_g000022 Rmu_sc0004080.1_g000011 Rmu_sc0004487.1_g000009 Rmu_sc0004988.1_g000018 Rmu_sc0004988.1_g000019 Rmu_sc0005254.1_g000002 Rmu_sc0005733.1_g000004 Rmu_sc0006413.1_g000006 Rmu_sc0007121.1_g000013 Rmu_sc0007869.1_g000006 Rmu_sc0008035.1_g000015 Rmu_sc0008279.1_g000017 Rmu_sc0008351.1_g000014 Rmu_sc0008957.1_g000007 Rmu_sc0009012.1_g000007 Rmu_sc0010556.1_g000009 Rmu_sc0012889.1_g000003 Rmu_sc0019808.1_g000004 Rmu_sc0028113.1_g000001 Rmu_sc0031202.1_g000001 Rmu_sc0034436.1_g000001 Rmu_sc0034445.1_g000001 Rmu_sc0040044.1_g000001 Rmu_ssc0000062.1_g000032 Rmu_ssc0000372.1_g000018
rosa_roxburghii Rroxscaffold_1G00001520 Rroxscaffold_1G00039990 Rroxscaffold_1G00046370 Rroxscaffold_2G00110140 Rroxscaffold_2G00131420 Rroxscaffold_3G00222390 Rroxscaffold_5G00336040 Rroxscaffold_5G00338000 Rroxscaffold_5G00344030 Rroxscaffold_5G00363460 Rroxscaffold_6G00402000 Rroxscaffold_7G00194940
rosa_rugosa Rorug01G0106500 Rorug01G0111300 Rorug01G0247000 Rorug02G0318100.1 Rorug02G0318200 Rorug02G0459500 Rorug03G0108100 Rorug03G0200500 Rorug03G0232500 Rorug03G0240600 Rorug04G0105300 Rorug04G0256600 Rorug06G0079300 Rorug06G0082700 Rorug06G0095600 Rorug06G0179000 Rorug07G0113500 Rorug07G0271100
rosa_samantha Rh1AG087200 Rh1CG085300 Rh1DG013200 Rh1DG091700 Rh1DG091800 Rh1DG338300 Rh2AG375400 Rh2AG407000 Rh2AG513500 Rh2BG277700 Rh2BG371200 Rh2BG376100 Rh2BG580500 Rh2CG354300 Rh2DG200100 Rh2DG388000 Rh2DG393500 Rh2DG426400 Rh3CG304800 Rh4AG044600 Rh4AG220500 Rh4BG379300 Rh4CG007100 Rh4CG030300 Rh4CG038000 Rh4CG114900 Rh4CG393700 Rh5AG124900 Rh5AG342300 Rh5BG414400 Rh5CG263800 Rh5CG439000 Rh5DG231100 Rh5DG566900 Rh6AG138600 Rh6AG200000 Rh6AG253100 Rh6AG390900 Rh6AG441600 Rh6BG194700 Rh6BG225800 Rh6BG256100 Rh6CG231700 Rh6CG231900 Rh6CG312200 Rh6CG453700 Rh6DG219400 Rh7AG357700 Rh7AG442000 Rh7CG425700 Rh7CG474600 Rh7CG494100 Rh7DG253900 Rh7DG294700 Rh7DG401600 Rh7DG461300
rosa_wichuraiana Rw1G001190 Rw2G047020 Rw4G003490 Rw5G039690 Rw6G010450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2062
Acc16I TGCGCA 1 cut(s) 896
Acc36I ACCTGC 2 cut(s) 2805, 3864
Acc65I GGTACC 1 cut(s) 3177
AccB1I GGYRCC 3 cut(s) 61, 187, 3177
AccB7I CCANNNNNTGG 1 cut(s) 1085
AccI GTMKAC 2 cut(s) 364, 512
AccII CGCG 4 cut(s) 238, 660, 3687, 3910
AccIII TCCGGA 1 cut(s) 268
AclWI GGATC 8 cut(s) 260, 273, 1048, 1129, 1355, 1537, 2846, 2933
AcoI YGGCCR 2 cut(s) 1705, 3128
AcsI RAATTY 7 cut(s) 710, 1007, 2695, 3431, 3543, 3754, 3808
AcvI CACGTG 1 cut(s) 3822
AcyI GRCGYC 1 cut(s) 1441
AdeI CACNNNGTG 1 cut(s) 281
AfaI GTAC 6 cut(s) 1073, 1357, 1411, 2101, 3148, 3179
AfeI AGCGCT 1 cut(s) 3714
AfiI CCNNNNNNNGG 4 cut(s) 664, 1085, 2044, 2117
AflII CTTAAG 1 cut(s) 1538
AgeI ACCGGT 1 cut(s) 1401
AhlI ACTAGT 1 cut(s) 3239
AjnI CCWGG 4 cut(s) 1036, 1768, 1786, 3648
AjuI GAANNNNNNNTTGG 2 cut(s) 1475, 1507
AleI CACNNNNGTG 1 cut(s) 437
AloI GAACNNNNNNTCC 2 cut(s) 1146, 1178
Alw21I GWGCWC 1 cut(s) 286
Alw26I GTCTC 9 cut(s) 964, 1513, 1728, 2127, 2221, 2833, 3341, 3977, 4002
Alw44I GTGCAC 1 cut(s) 282
AlwI GGATC 8 cut(s) 260, 273, 1048, 1129, 1355, 1537, 2846, 2933
Aor13HI TCCGGA 1 cut(s) 268
Aor51HI AGCGCT 1 cut(s) 3714
AoxI GGCC 9 cut(s) 655, 1217, 1705, 1709, 1772, 1789, 2295, 3128, 3964
ApaLI GTGCAC 1 cut(s) 282
ApeKI GCWGC 5 cut(s) 1100, 1739, 2684, 3926, 3938
ApoI RAATTY 7 cut(s) 710, 1007, 2695, 3431, 3543, 3754, 3808
ArsI GACNNNNNNTTYG 2 cut(s) 591, 623
AsiGI ACCGGT 1 cut(s) 1401
Asp700I GAANNNNTTC 1 cut(s) 1607
Asp718I GGTACC 1 cut(s) 3177
AspLEI GCGC 4 cut(s) 240, 897, 3689, 3715
AsuC2I CCSGG 2 cut(s) 2466, 3680
AsuHPI GGTGA 7 cut(s) 207, 701, 1100, 1487, 2044, 2428, 3646
AsuNHI GCTAGC 3 cut(s) 1879, 3689, 3715
AvaII GGWCC 6 cut(s) 599, 1180, 1254, 2794, 3109, 3682
BaeGI GKGCMC 3 cut(s) 22, 286, 3374
BalI TGGCCA 1 cut(s) 3130
BamHI GGATCC 1 cut(s) 265
BanI GGYRCC 3 cut(s) 61, 187, 3177
BanII GRGCYC 3 cut(s) 148, 1141, 2484
BbrPI CACGTG 1 cut(s) 3822
BbsI GAAGAC 3 cut(s) 212, 545, 3213
Bbv12I GWGCWC 1 cut(s) 286
BbvCI CCTCAGC 1 cut(s) 1421
BbvI GCAGC 5 cut(s) 1087, 1751, 2671, 3913, 3950
BceAI ACGGC 3 cut(s) 40, 166, 3728
BciT130I CCWGG 4 cut(s) 1038, 1770, 1788, 3650
BciVI GTATCC 4 cut(s) 115, 1527, 3010, 3897
BclI TGATCA 5 cut(s) 457, 1558, 2356, 2410, 2458
BcnI CCSGG 2 cut(s) 2466, 3680
BcoDI GTCTC 9 cut(s) 964, 1513, 1728, 2127, 2221, 2833, 3341, 3977, 4002
BcuI ACTAGT 1 cut(s) 3239
BfmI CTRYAG 2 cut(s) 2058, 3696
BfoI RGCGCY 1 cut(s) 3716
BfrI CTTAAG 1 cut(s) 1538
BfuAI ACCTGC 2 cut(s) 2805, 3864
BfuI GTATCC 4 cut(s) 115, 1527, 3010, 3897
BglII AGATCT 4 cut(s) 671, 1390, 1448, 3802
BisI GCNGC 7 cut(s) 658, 1101, 1740, 2685, 3486, 3927, 3939
BlpI GCTNAGC 2 cut(s) 326, 2826
BlsI GCNGC 7 cut(s) 659, 1102, 1741, 2686, 3487, 3928, 3940
BmcAI AGTACT 1 cut(s) 2101
Bme1390I CCNGG 6 cut(s) 1038, 1770, 1788, 2466, 3650, 3680
Bme18I GGWCC 6 cut(s) 599, 1180, 1254, 2794, 3109, 3682
BmrFI CCNGG 6 cut(s) 1038, 1770, 1788, 2466, 3650, 3680
BmrI ACTGGG 2 cut(s) 914, 2730
BmsI GCATC 7 cut(s) 262, 410, 1642, 2431, 3040, 3323, 3981
BmtI GCTAGC 3 cut(s) 1883, 3693, 3719
BmuI ACTGGG 2 cut(s) 914, 2730
BoxI GACNNNNGTC 1 cut(s) 1185
BpiI GAAGAC 3 cut(s) 212, 545, 3213
BplI GAGNNNNNCTC 4 cut(s) 2124, 2156, 2968, 3000
BpmI CTGGAG 2 cut(s) 1752, 3916
Bpu10I CCTNAGC 1 cut(s) 1421
Bpu1102I GCTNAGC 2 cut(s) 326, 2826
BpuEI CTTGAG 3 cut(s) 470, 711, 2565
BpuMI CCSGG 2 cut(s) 2466, 3680
BsaAI YACGTR 1 cut(s) 3822
BsaBI GATNNNNATC 2 cut(s) 1557, 3581
BsaHI GRCGYC 1 cut(s) 1441
BsaI GGTCTC 2 cut(s) 964, 2127
BsaJI CCNNGG 7 cut(s) 86, 371, 658, 1036, 1769, 3649, 3678
BsaWI WCCGGW 4 cut(s) 268, 1401, 1530, 2941
BsaXI ACNNNNNCTCC 2 cut(s) 2877, 2907
Bsc4I CCNNNNNNNGG 4 cut(s) 664, 1085, 2044, 2117
Bse118I RCCGGY 2 cut(s) 1401, 1707
Bse8I GATNNNNATC 2 cut(s) 1557, 3581
BseAI TCCGGA 1 cut(s) 268
BseBI CCWGG 4 cut(s) 1038, 1770, 1788, 3650
BseDI CCNNGG 7 cut(s) 86, 371, 658, 1036, 1769, 3649, 3678
BseJI GATNNNNATC 2 cut(s) 1557, 3581
BseLI CCNNNNNNNGG 4 cut(s) 664, 1085, 2044, 2117
BseMII CTCAG 5 cut(s) 317, 1412, 1918, 3483, 3813
BseRI GAGGAG 8 cut(s) 20, 146, 497, 563, 607, 1442, 2224, 2294
BseSI GKGCMC 3 cut(s) 22, 286, 3374
BseX3I CGGCCG 1 cut(s) 1705
BseXI GCAGC 5 cut(s) 1087, 1751, 2671, 3913, 3950
Bsh1236I CGCG 4 cut(s) 238, 660, 3687, 3910
Bsh1285I CGRYCG 1 cut(s) 1708
BshFI GGCC 9 cut(s) 657, 1219, 1707, 1711, 1774, 1791, 2297, 3130, 3966
BshNI GGYRCC 3 cut(s) 61, 187, 3177
BshTI ACCGGT 1 cut(s) 1401
BsiEI CGRYCG 1 cut(s) 1708
BsiHKAI GWGCWC 1 cut(s) 286
BsiSI CCGG 7 cut(s) 269, 1402, 1531, 1708, 2465, 2942, 3680
BslFI GGGAC 4 cut(s) 2229, 2239, 2731, 2807
BslI CCNNNNNNNGG 4 cut(s) 664, 1085, 2044, 2117
BsmAI GTCTC 9 cut(s) 964, 1513, 1728, 2127, 2221, 2833, 3341, 3977, 4002
BsmBI CGTCTC 2 cut(s) 1728, 4002
BsmFI GGGAC 4 cut(s) 2229, 2239, 2731, 2807
BsmI GAATGC 1 cut(s) 3316
BsnI GGCC 9 cut(s) 657, 1219, 1707, 1711, 1774, 1791, 2297, 3130, 3966
Bso31I GGTCTC 2 cut(s) 964, 2127
Bsp1286I GDGCHC 6 cut(s) 22, 148, 286, 1141, 2484, 3374
Bsp13I TCCGGA 1 cut(s) 268
Bsp1407I TGTACA 1 cut(s) 1071
Bsp1720I GCTNAGC 2 cut(s) 326, 2826
Bsp19I CCATGG 2 cut(s) 86, 371
BspANI GGCC 9 cut(s) 657, 1219, 1707, 1711, 1774, 1791, 2297, 3130, 3966
BspCNI CTCAG 5 cut(s) 318, 1413, 1917, 3484, 3812
BspEI TCCGGA 1 cut(s) 268
BspFNI CGCG 4 cut(s) 238, 660, 3687, 3910
BspHI TCATGA 2 cut(s) 2289, 4039
BspMI ACCTGC 2 cut(s) 2805, 3864
BspOI GCTAGC 3 cut(s) 1883, 3693, 3719
BspPI GGATC 8 cut(s) 260, 273, 1048, 1129, 1355, 1537, 2846, 2933
BspQI GCTCTTC 1 cut(s) 3863
BspT107I GGYRCC 3 cut(s) 61, 187, 3177
BspTI CTTAAG 1 cut(s) 1538
BspTNI GGTCTC 2 cut(s) 964, 2127
BsrFI RCCGGY 2 cut(s) 1401, 1707
BsrGI TGTACA 1 cut(s) 1071
BssAI RCCGGY 2 cut(s) 1401, 1707
BssECI CCNNGG 7 cut(s) 86, 371, 658, 1036, 1769, 3649, 3678
BssNAI GTATAC 1 cut(s) 365
BssNI GRCGYC 1 cut(s) 1441
BssT1I CCWWGG 2 cut(s) 86, 371
Bst1107I GTATAC 1 cut(s) 365
Bst2UI CCWGG 4 cut(s) 1038, 1770, 1788, 3650
Bst6I CTCTTC 7 cut(s) 625, 1515, 1799, 2007, 2187, 2994, 3863
BstACI GRCGYC 1 cut(s) 1441
BstAFI CTTAAG 1 cut(s) 1538
BstAPI GCANNNNNTGC 2 cut(s) 281, 2822
BstAUI TGTACA 1 cut(s) 1071
BstBAI YACGTR 1 cut(s) 3822
BstDEI CTNAG 9 cut(s) 326, 812, 1421, 1904, 2826, 3138, 3332, 3492, 3799
BstDSI CCRYGG 3 cut(s) 86, 371, 658
BstFNI CGCG 4 cut(s) 238, 660, 3687, 3910
BstH2I RGCGCY 1 cut(s) 3716
BstHHI GCGC 4 cut(s) 240, 897, 3689, 3715
BstMAI GTCTC 9 cut(s) 964, 1513, 1728, 2127, 2221, 2833, 3341, 3977, 4002
BstMCI CGRYCG 1 cut(s) 1708
BstNI CCWGG 4 cut(s) 1038, 1770, 1788, 3650
BstNSI RCATGY 1 cut(s) 2052
BstPAI GACNNNNGTC 1 cut(s) 1185
BstSCI CCNGG 6 cut(s) 1036, 1768, 1786, 2464, 3648, 3678
BstSFI CTRYAG 2 cut(s) 2058, 3696
BstSLI GKGCMC 3 cut(s) 22, 286, 3374
BstUI CGCG 4 cut(s) 238, 660, 3687, 3910
BstV1I GCAGC 5 cut(s) 1087, 1751, 2671, 3913, 3950
BstV2I GAAGAC 3 cut(s) 212, 545, 3213
BstX2I RGATCY 6 cut(s) 265, 671, 1390, 1448, 1542, 3802
BstXI CCANNNNNNTGG 2 cut(s) 2493, 2963
BstYI RGATCY 6 cut(s) 265, 671, 1390, 1448, 1542, 3802
BstZ17I GTATAC 1 cut(s) 365
BstZI CGGCCG 1 cut(s) 1705
BsuI GTATCC 4 cut(s) 115, 1527, 3010, 3897
BsuRI GGCC 9 cut(s) 657, 1219, 1707, 1711, 1774, 1791, 2297, 3130, 3966
BtgI CCRYGG 3 cut(s) 86, 371, 658
BtsI GCAGTG 1 cut(s) 3711
BtsIMutI CAGTG 9 cut(s) 284, 1213, 1568, 2067, 2454, 2734, 3172, 3711, 3892
BveI ACCTGC 2 cut(s) 2805, 3864
CciI TCATGA 2 cut(s) 2289, 4039
CfoI GCGC 4 cut(s) 240, 897, 3689, 3715
Cfr10I RCCGGY 2 cut(s) 1401, 1707
Cfr42I CCGCGG 1 cut(s) 661
CpoI CGGWCCG 1 cut(s) 3682
CseI GACGC 3 cut(s) 1430, 1745, 3721
Csp6I GTAC 6 cut(s) 1072, 1356, 1410, 2100, 3147, 3178
CspAI ACCGGT 1 cut(s) 1401
CspI CGGWCCG 1 cut(s) 3682
CviQI GTAC 6 cut(s) 1072, 1356, 1410, 2100, 3147, 3178
DdeI CTNAG 9 cut(s) 326, 812, 1421, 1904, 2826, 3138, 3332, 3492, 3799
DraI TTTAAA 4 cut(s) 198, 1194, 1944, 2679
DraIII CACNNNGTG 1 cut(s) 281
DrdI GACNNNNNNGTC 1 cut(s) 2062
DseDI GACNNNNNNGTC 1 cut(s) 2062
EaeI YGGCCR 2 cut(s) 1705, 3128
EagI CGGCCG 1 cut(s) 1705
Eam1104I CTCTTC 7 cut(s) 625, 1515, 1799, 2007, 2187, 2994, 3863
EarI CTCTTC 7 cut(s) 625, 1515, 1799, 2007, 2187, 2994, 3863
EciI GGCGGA 1 cut(s) 2910
EclXI CGGCCG 1 cut(s) 1705
Eco130I CCWWGG 2 cut(s) 86, 371
Eco147I AGGCCT 1 cut(s) 1791
Eco24I GRGCYC 3 cut(s) 148, 1141, 2484
Eco31I GGTCTC 2 cut(s) 964, 2127
Eco32I GATATC 1 cut(s) 2322
Eco47I GGWCC 6 cut(s) 599, 1180, 1254, 2794, 3109, 3682
Eco47III AGCGCT 1 cut(s) 3714
Eco52I CGGCCG 1 cut(s) 1705
Eco72I CACGTG 1 cut(s) 3822
EcoO109I RGGNCCY 2 cut(s) 2794, 3109
EcoRII CCWGG 4 cut(s) 1036, 1768, 1786, 3648
EcoRV GATATC 1 cut(s) 2322
EcoT14I CCWWGG 2 cut(s) 86, 371
EcoT22I ATGCAT 1 cut(s) 3316
EcoT38I GRGCYC 3 cut(s) 148, 1141, 2484
ErhI CCWWGG 2 cut(s) 86, 371
Esp3I CGTCTC 2 cut(s) 1728, 4002
FalI AAGNNNNNCTT 4 cut(s) 1825, 1857, 1986, 2018
FaqI GGGAC 4 cut(s) 2229, 2239, 2731, 2807
FauI CCCGC 4 cut(s) 500, 940, 1321, 1465
FauNDI CATATG 3 cut(s) 1366, 2900, 3186
FbaI TGATCA 5 cut(s) 457, 1558, 2356, 2410, 2458
FblI GTMKAC 2 cut(s) 364, 512
Fnu4HI GCNGC 7 cut(s) 658, 1101, 1740, 2685, 3486, 3927, 3939
FriOI GRGCYC 3 cut(s) 148, 1141, 2484
FseI GGCCGGCC 1 cut(s) 1711
Fsp4HI GCNGC 7 cut(s) 658, 1101, 1740, 2685, 3486, 3927, 3939
FspI TGCGCA 1 cut(s) 896
GlaI GCGC 4 cut(s) 239, 896, 3688, 3714
GluI GCNGC 7 cut(s) 658, 1101, 1740, 2685, 3486, 3927, 3939
GsuI CTGGAG 2 cut(s) 1752, 3916
HaeII RGCGCY 1 cut(s) 3716
HaeIII GGCC 9 cut(s) 657, 1219, 1707, 1711, 1774, 1791, 2297, 3130, 3966
HapII CCGG 7 cut(s) 269, 1402, 1531, 1708, 2465, 2942, 3680
HgaI GACGC 3 cut(s) 1430, 1745, 3721
HhaI GCGC 4 cut(s) 240, 897, 3689, 3715
Hin1I GRCGYC 1 cut(s) 1441
Hin6I GCGC 4 cut(s) 238, 895, 3687, 3713
HinP1I GCGC 4 cut(s) 238, 895, 3687, 3713
HincII GTYRAC 2 cut(s) 3195, 3748
HindII GTYRAC 2 cut(s) 3195, 3748
HindIII AAGCTT 4 cut(s) 230, 587, 1962, 2832
HpaII CCGG 7 cut(s) 269, 1402, 1531, 1708, 2465, 2942, 3680
HphI GGTGA 7 cut(s) 207, 701, 1100, 1487, 2044, 2428, 3646
Hpy99I CGWCG 1 cut(s) 3123
HpyAV CCTTC 9 cut(s) 92, 617, 1858, 1909, 2287, 2493, 2530, 2641, 3439
HpyCH4IV ACGT 1 cut(s) 3821
HpyF3I CTNAG 9 cut(s) 326, 812, 1421, 1904, 2826, 3138, 3332, 3492, 3799
HpySE526I ACGT 1 cut(s) 3821
Hsp92I GRCGYC 1 cut(s) 1441
HspAI GCGC 4 cut(s) 238, 895, 3687, 3713
Kpn2I TCCGGA 1 cut(s) 268
KpnI GGTACC 1 cut(s) 3181
KroI GCCGGC 1 cut(s) 1707
KroNI GCCGGC 1 cut(s) 1709
Ksp22I TGATCA 5 cut(s) 457, 1558, 2356, 2410, 2458
KspI CCGCGG 1 cut(s) 661
LguI GCTCTTC 1 cut(s) 3863
LmnI GCTCC 6 cut(s) 1754, 1771, 2487, 2989, 3205, 3279
Lsp1109I GCAGC 5 cut(s) 1087, 1751, 2671, 3913, 3950
LweI GCATC 7 cut(s) 262, 410, 1642, 2431, 3040, 3323, 3981
MaeII ACGT 1 cut(s) 3821
MfeI CAATTG 1 cut(s) 3586
MflI RGATCY 6 cut(s) 265, 671, 1390, 1448, 1542, 3802
MhlI GDGCHC 6 cut(s) 22, 148, 286, 1141, 2484, 3374
MlsI TGGCCA 1 cut(s) 3130
MluNI TGGCCA 1 cut(s) 3130
MlyI GAGTC 7 cut(s) 17, 143, 967, 1315, 2572, 2986, 3979
MmeI TCCRAC 5 cut(s) 1158, 1184, 1281, 1636, 3960
Mox20I TGGCCA 1 cut(s) 3130
Mph1103I ATGCAT 1 cut(s) 3316
MroI TCCGGA 1 cut(s) 268
MroNI GCCGGC 1 cut(s) 1707
MroXI GAANNNNTTC 1 cut(s) 1607
MscI TGGCCA 1 cut(s) 3130
MslI CAYNNNNRTG 2 cut(s) 437, 870
Msp20I TGGCCA 1 cut(s) 3130
MspA1I CMGCKG 2 cut(s) 660, 947
MspCI CTTAAG 1 cut(s) 1538
MspI CCGG 7 cut(s) 269, 1402, 1531, 1708, 2465, 2942, 3680
MspR9I CCNGG 6 cut(s) 1038, 1770, 1788, 2466, 3650, 3680
MunI CAATTG 1 cut(s) 3586
Mva1269I GAATGC 1 cut(s) 3316
MvaI CCWGG 4 cut(s) 1038, 1770, 1788, 3650
MvnI CGCG 4 cut(s) 238, 660, 3687, 3910
NaeI GCCGGC 1 cut(s) 1709
NciI CCSGG 2 cut(s) 2466, 3680
NcoI CCATGG 2 cut(s) 86, 371
NdeI CATATG 3 cut(s) 1366, 2900, 3186
NgoMIV GCCGGC 1 cut(s) 1707
NheI GCTAGC 3 cut(s) 1879, 3689, 3715
NmeAIII GCCGAG 2 cut(s) 1448, 1683
NmuCI GTSAC 7 cut(s) 9, 135, 304, 715, 775, 1307, 2053
NsbI TGCGCA 1 cut(s) 896
NsiI ATGCAT 1 cut(s) 3316
NspI RCATGY 1 cut(s) 2052
OliI CACNNNNGTG 1 cut(s) 437
PagI TCATGA 2 cut(s) 2289, 4039
PceI AGGCCT 1 cut(s) 1791
PciSI GCTCTTC 1 cut(s) 3863
PctI GAATGC 1 cut(s) 3316
PdiI GCCGGC 1 cut(s) 1709
PdmI GAANNNNTTC 1 cut(s) 1607
PfeI GAWTC 9 cut(s) 692, 1455, 1478, 1603, 2276, 3073, 3524, 3556, 3782
PflFI GACNNNGTC 1 cut(s) 639
PflMI CCANNNNNTGG 1 cut(s) 1085
PinAI ACCGGT 1 cut(s) 1401
PkrI GCNGC 7 cut(s) 659, 1102, 1741, 2686, 3487, 3928, 3940
PleI GAGTC 7 cut(s) 16, 142, 966, 1314, 2572, 2985, 3979
PmaCI CACGTG 1 cut(s) 3822
PmlI CACGTG 1 cut(s) 3822
PpsI GAGTC 7 cut(s) 16, 142, 966, 1314, 2572, 2985, 3979
Ppu21I YACGTR 1 cut(s) 3822
PpuMI RGGWCCY 2 cut(s) 2794, 3109
PshAI GACNNNNGTC 1 cut(s) 1185
Psp5II RGGWCCY 2 cut(s) 2794, 3109
Psp6I CCWGG 4 cut(s) 1036, 1768, 1786, 3648
PspCI CACGTG 1 cut(s) 3822
PspGI CCWGG 4 cut(s) 1036, 1768, 1786, 3648
PspPPI RGGWCCY 2 cut(s) 2794, 3109
PsuI RGATCY 6 cut(s) 265, 671, 1390, 1448, 1542, 3802
PsyI GACNNNGTC 1 cut(s) 639
RigI GGCCGGCC 1 cut(s) 1711
RsaI GTAC 6 cut(s) 1073, 1357, 1411, 2101, 3148, 3179
RsaNI GTAC 6 cut(s) 1072, 1356, 1410, 2100, 3147, 3178
RseI CAYNNNNRTG 2 cut(s) 437, 870
Rsr2I CGGWCCG 1 cut(s) 3682
RsrII CGGWCCG 1 cut(s) 3682
SacII CCGCGG 1 cut(s) 661
SapI GCTCTTC 1 cut(s) 3863
SatI GCNGC 7 cut(s) 658, 1101, 1740, 2685, 3486, 3927, 3939
ScaI AGTACT 1 cut(s) 2101
SchI GAGTC 7 cut(s) 17, 143, 967, 1315, 2572, 2986, 3979
ScrFI CCNGG 6 cut(s) 1038, 1770, 1788, 2466, 3650, 3680
SduI GDGCHC 6 cut(s) 22, 148, 286, 1141, 2484, 3374
SfaNI GCATC 7 cut(s) 262, 410, 1642, 2431, 3040, 3323, 3981
SfcI CTRYAG 2 cut(s) 2058, 3696
Sfr303I CCGCGG 1 cut(s) 661
SgrBI CCGCGG 1 cut(s) 661
SinI GGWCC 6 cut(s) 599, 1180, 1254, 2794, 3109, 3682
SmiI ATTTAAAT 1 cut(s) 2679
SmiMI CAYNNNNRTG 2 cut(s) 437, 870
SmlI CTYRAG 4 cut(s) 449, 726, 1538, 2580
SmoI CTYRAG 4 cut(s) 449, 726, 1538, 2580
SpeI ACTAGT 1 cut(s) 3239
SseBI AGGCCT 1 cut(s) 1791
SspI AATATT 2 cut(s) 1873, 2386
StuI AGGCCT 1 cut(s) 1791
StyD4I CCNGG 6 cut(s) 1036, 1768, 1786, 2464, 3648, 3678
StyI CCWWGG 2 cut(s) 86, 371
SwaI ATTTAAAT 1 cut(s) 2679
TaiI ACGT 1 cut(s) 3824
TaqI TCGA 9 cut(s) 223, 735, 1057, 1124, 2381, 3022, 3121, 3402, 3506
TatI WGTACW 2 cut(s) 1071, 2099
TauI GCSGC 2 cut(s) 660, 3488
TfiI GAWTC 9 cut(s) 692, 1455, 1478, 1603, 2276, 3073, 3524, 3556, 3782
TscAI CASTG 9 cut(s) 291, 1220, 1568, 2067, 2461, 2741, 3179, 3711, 3899
TseFI GTSAC 7 cut(s) 9, 135, 304, 715, 775, 1307, 2053
TseI GCWGC 5 cut(s) 1100, 1739, 2684, 3926, 3938
Tsp45I GTSAC 7 cut(s) 9, 135, 304, 715, 775, 1307, 2053
TspGWI ACGGA 4 cut(s) 664, 1298, 1448, 1794
TspRI CASTG 9 cut(s) 291, 1220, 1568, 2067, 2461, 2741, 3179, 3711, 3899
Tth111I GACNNNGTC 1 cut(s) 639
Van91I CCANNNNNTGG 1 cut(s) 1085
Vha464I CTTAAG 1 cut(s) 1538
VneI GTGCAC 1 cut(s) 282
VpaK11BI GGWCC 6 cut(s) 599, 1180, 1254, 2794, 3109, 3682
XapI RAATTY 7 cut(s) 710, 1007, 2695, 3431, 3543, 3754, 3808
XbaI TCTAGA 2 cut(s) 499, 1451
XceI RCATGY 1 cut(s) 2052
XcmI CCANNNNNNNNNTGG 1 cut(s) 541
XmiI GTMKAC 2 cut(s) 364, 512
XmnI GAANNNNTTC 1 cut(s) 1607
ZrmI AGTACT 1 cut(s) 2101
Zsp2I ATGCAT 1 cut(s) 3316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.