Rw6G010450

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000147
Physical Location & Seq
Reverse (-)
18058915 .. 18062027
3113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G010450.1

Sequence Viewer

Length: 2304 bp
ATGCAACCCCAGCAAAACCCAAATCCTATCCCAGAAGACCCGGTCATTGACCTCAATCAACCGGAGATTGAACCGGCCCAACCAACAACTTTGCTGGGTTTCATCATTGCTGAAAAATTCCTCAATGTGGCTGCTGTTATCAGGATCTTGGCTCGTGCTTGGGAAAACTTTGGTGAAGTTCACATTGAATCCATCCGAGACAACCGAACCTTTTCCATCACCCCTTTGGCCCCAGACGGGGGTCAACTTATCCTTGACCGGGGTCCTTGGTCCGTCATGGGTCACACGGTCAGCGTTCACCTCTGGCCTCTTGAGGCTCGACTTGAGGATGTCCCCCTCCACCTCCTATCCATCTGGATCCAAGCTCATGGCCTCCAACGGAGCCAAATGACAACTGAAAATGCCCACCTTCTTGGTGCAACTGCTGGTACTGTCCTGGAGGTGGAAAACCCCAATGTTGTCAACTGTTGGAGGGGTTTTCTCCGTTTTCGTGTTTCCATCGACTCTAGGCACCCAATCACTCCTGGGGTCTGGATCCGTTGTGGATCACGTCCACCCATTTGGGTTGAGTTCAGGTATGAAAGAATATCTCTGTTTTGCTATAATTGTGGTCAACTTGGTCACAGTGCTAGGTTCTGCAATCATGAACGCCACCCCAGAGCGGAGACAATGGGTGTTTGGATGTTGTCCCCGGCAGCTCGACGCCTTATTGTTACCCCTTCTGAGGGAGAACAGGGTTGGGACCTCTTTCAACGTCAGAACTTTCAGAATCGACAAAATCGCTGGGCTTTCTCAGACCCCCCAACCCCTGTCACTACCCACCTGCTGCAAACAACCGATCACCCTGGCCCTTCTTCTTTCCCTACCCTTGGTCCTTCGGGTGAACTCTCAATGGGAAGGACCCACTTTTTCTTCGATACAAACACAAATGACTTCATTCCCATAACCCAAGATACTCAACTTTACACGTCACAATCCTCAATCCAAACCCAATCTGCTCCCCTTCATACCTCTCCCCAACATTCCACACACCCCCAGAATTTGGTACCTCCAACAGACCTTTTTCTGTCTACCCCAGTAAACTCATTGACACCCTCCTCACCTTTAACACCCACTGTCCCACATTCACCGACCCACTCTGATAACTCCATCACTGGGATTGCGACGTTTCTGGATCGCCTTCGTCTCAAGAGACAAGCTGAAGAGGCAGCCCTCCTTTCCACTACTCCGGTTAAAAAGCAAAAGATGGGATCTCTACCCCACGGGGTCACCATGAGGCTACTATCTTGGAATTGTCAGGGTCTAGGGGCTGACCTGACTGGGAAACATCTGAAACGTTTGATTAAACGTAACAGCCCCTCCATGCTTTTTCTAATGGAAACTCGTCAGAACGAATCCACCATCCTATCTTGGAAAAAGACATTGAATTTTGACCATGTTTACGTGGTTGACCCGGTGGGTCAGAGCAGTGGTGGGCTAGCTCTGTTTTGGGACAACTCTGTCCAAGTCTCCAGTATCACAAGCACGCATAACTATATTTCTACTTCTGTACTTTCTGTTGCGGACTCTTTCCTTTGTAATATCTCTTGGATGTATGGGAACCCCCACTCTAATCAAAAAAATGCATTCTGGCGTTCTGTTTATACTGCTTTCTTCCCCAGATTAATTCCTTGGTTGTGTTTAGGAGACTTCAATGAAATTATGTGGCTCCATGAGAAGTGGGGAGGTTTACCTCAACCAAGATGGAGGCTGAACCTCTTTCGGGAGTTCCTCTCACACACTGAACTTTCTGATCTCCAGTTTCAAGGACCAGACTACACTTGGTTCTGCATCCAACATGGCAGAATCGTCATTAAAGAGAGATTGGACAGGGGATTGGCAAATAATTCTTGGATCGCTGCCAAACCCTGTTCTCAGATCTTTCACTTACCCAAACTCGGATCTGATCATCGTCCAATCCTTTTCGATTCCTCTCCCCCGGAGGTCAAAGCCCCTCACGTGTTTCGGTTTGAACATATATGGACCTCCCATCCAGACAGCTTTCAGGTGGTCTCCTCTAATTGGTCCACTACTCCAACAACATCTGCATCCCAAACTTGGGAAGATAACTTGAATCGGTGTAAGTCTTCACTCACCACATGGGCCTCTAATACTTTTCCAAACTTTGCAAAGAAAGTCTCCACCGAAACCCAGTTGATTCAAGACGTCCTACAGTCTGACCATACTGACCAAGTTGACCAACCATGTGCGCAAGAGTCCATCCGTCAGGCATCTGACAATATCTTGTCCTTGTGGTCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 Exo_endo_phos_2 RNase_H RVT_1 zf-CCHC_4

Protein Analysis

767

Amino Acids

86.87

Weight (kDa)

6.7

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 167 - 214 5.2e-13 Zinc knuckle
Exo_endo_phos PF03372 428 - 650 1.1e-11 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000147)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10354 FvH4_1g17131 FvH4_2g11611 FvH4_2g33851 FvH4_3g32112 FvH4_3g35052 FvH4_3g44611 FvH4_4g05921 FvH4_4g06411 FvH4_4g06792 FvH4_4g10365 FvH4_4g21422 FvH4_4g22401 FvH4_4g30261 FvH4_5g08843 FvH4_5g14731 FvH4_5g24781 FvH4_5g26231 FvH4_5g31282 FvH4_6g23371 FvH4_6g38511 FvH4_7g01171 FvH4_7g03341 FvH4_7g06171 FvH4_7g32472
prunus_persica Prupe.2G036900_v2.0.a1
pyrus_communis pycom02g19900 pycom03g01520 pycom05g10510 pycom16g23820
rosa_chinensis RchiOBHm_Chr1g0316031 RchiOBHm_Chr1g0342451 RchiOBHm_Chr1g0344541 RchiOBHm_Chr1g0364721 RchiOBHm_Chr2g0133541 RchiOBHm_Chr2g0134511 RchiOBHm_Chr2g0145891 RchiOBHm_Chr2g0163241 RchiOBHm_Chr5g0027201 RchiOBHm_Chr5g0027881 RchiOBHm_Chr5g0047471 RchiOBHm_Chr7g0210901
rosa_laevigata RLG00000011116 RLG00000017964 RLG00000018128 RLG00000018387 RLG00000018829 RLG00000019353 RLG00000028973 RLG00000035870 RLG00000036629
rosa_multiflora Rmu_co8126340.1_g000001 Rmu_co8216750.1_g000001 Rmu_co8222072.1_g000001 Rmu_co8332453.1_g000001 Rmu_co8349913.1_g000001 Rmu_co8405337.1_g000001 Rmu_sc0000041.1_g000012 Rmu_sc0000166.1_g000029 Rmu_sc0000251.1_g000019 Rmu_sc0000271.1_g000028 Rmu_sc0000509.1_g000036 Rmu_sc0000543.1_g000048 Rmu_sc0000808.1_g000003 Rmu_sc0000847.1_g000040 Rmu_sc0000972.1_g000020 Rmu_sc0001260.1_g000001 Rmu_sc0001464.1_g000026 Rmu_sc0001616.1_g000003 Rmu_sc0001669.1_g000001 Rmu_sc0002005.1_g000001 Rmu_sc0002014.1_g000010 Rmu_sc0002082.1_g000033 Rmu_sc0002302.1_g000024 Rmu_sc0002531.1_g000045 Rmu_sc0003276.1_g000002 Rmu_sc0003737.1_g000007 Rmu_sc0003832.1_g000022 Rmu_sc0004080.1_g000011 Rmu_sc0004487.1_g000009 Rmu_sc0004988.1_g000018 Rmu_sc0004988.1_g000019 Rmu_sc0005254.1_g000002 Rmu_sc0005733.1_g000004 Rmu_sc0006413.1_g000006 Rmu_sc0007121.1_g000013 Rmu_sc0007869.1_g000006 Rmu_sc0008035.1_g000015 Rmu_sc0008279.1_g000017 Rmu_sc0008351.1_g000014 Rmu_sc0008957.1_g000007 Rmu_sc0009012.1_g000007 Rmu_sc0010556.1_g000009 Rmu_sc0012889.1_g000003 Rmu_sc0019808.1_g000004 Rmu_sc0028113.1_g000001 Rmu_sc0031202.1_g000001 Rmu_sc0034436.1_g000001 Rmu_sc0034445.1_g000001 Rmu_sc0040044.1_g000001 Rmu_ssc0000062.1_g000032 Rmu_ssc0000372.1_g000018
rosa_roxburghii Rroxscaffold_1G00001520 Rroxscaffold_1G00039990 Rroxscaffold_1G00046370 Rroxscaffold_2G00110140 Rroxscaffold_2G00131420 Rroxscaffold_3G00222390 Rroxscaffold_5G00336040 Rroxscaffold_5G00338000 Rroxscaffold_5G00344030 Rroxscaffold_5G00363460 Rroxscaffold_6G00402000 Rroxscaffold_7G00194940
rosa_rugosa Rorug01G0106500 Rorug01G0111300 Rorug01G0247000 Rorug02G0318100.1 Rorug02G0318200 Rorug02G0459500 Rorug03G0108100 Rorug03G0200500 Rorug03G0232500 Rorug03G0240600 Rorug04G0105300 Rorug04G0256600 Rorug06G0079300 Rorug06G0082700 Rorug06G0095600 Rorug06G0179000 Rorug07G0113500 Rorug07G0271100
rosa_samantha Rh1AG087200 Rh1CG085300 Rh1DG013200 Rh1DG091700 Rh1DG091800 Rh1DG338300 Rh2AG375400 Rh2AG407000 Rh2AG513500 Rh2BG277700 Rh2BG371200 Rh2BG376100 Rh2BG580500 Rh2CG354300 Rh2DG200100 Rh2DG388000 Rh2DG393500 Rh2DG426400 Rh3CG304800 Rh4AG044600 Rh4AG220500 Rh4BG379300 Rh4CG007100 Rh4CG030300 Rh4CG038000 Rh4CG114900 Rh4CG393700 Rh5AG124900 Rh5AG342300 Rh5BG414400 Rh5CG263800 Rh5CG439000 Rh5DG231100 Rh5DG566900 Rh6AG138600 Rh6AG200000 Rh6AG253100 Rh6AG390900 Rh6AG441600 Rh6BG194700 Rh6BG225800 Rh6BG256100 Rh6CG231700 Rh6CG231900 Rh6CG312200 Rh6CG453700 Rh6DG219400 Rh7AG357700 Rh7AG442000 Rh7CG425700 Rh7CG474600 Rh7CG494100 Rh7DG253900 Rh7DG294700 Rh7DG401600 Rh7DG461300
rosa_wichuraiana Rw1G001190 Rw2G047020 Rw4G003490 Rw5G039690 Rw6G010450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 831
AasI GACNNNNNNGTC 1 cut(s) 1499
AatII GACGTC 1 cut(s) 2208
Acc16I TGCGCA 1 cut(s) 2250
Acc36I ACCTGC 1 cut(s) 831
Acc65I GGTACC 1 cut(s) 1045
AccB1I GGYRCC 2 cut(s) 510, 1045
AccB7I CCANNNNNTGG 1 cut(s) 1042
AccBSI CCGCTC 1 cut(s) 662
AccI GTMKAC 1 cut(s) 1070
AciI CCGC 2 cut(s) 662, 1562
AclI AACGTT 1 cut(s) 1336
AcsI RAATTY 3 cut(s) 116, 1039, 1426
AcuI CTGAAG 1 cut(s) 1221
AcvI CACGTG 1 cut(s) 1999
AcyI GRCGYC 2 cut(s) 703, 2205
AfaI GTAC 3 cut(s) 430, 1047, 1551
AflIII ACRYGT 2 cut(s) 966, 1998
AgsI TTSAA 9 cut(s) 71, 188, 752, 1426, 1693, 1805, 2012, 2113, 2201
AjiI CACGTC 2 cut(s) 551, 969
AjnI CCWGG 3 cut(s) 435, 523, 844
AluBI AGCT 5 cut(s) 365, 698, 1199, 1481, 2040
AluI AGCT 5 cut(s) 365, 698, 1199, 1481, 2040
Alw26I GTCTC 8 cut(s) 192, 659, 1186, 1190, 1513, 1680, 2056, 2182
AoxI GGCC 6 cut(s) 75, 228, 305, 370, 847, 2142
ApeKI GCWGC 5 cut(s) 131, 695, 826, 1208, 1898
ApoI RAATTY 3 cut(s) 116, 1039, 1426
AseI ATTAAT 1 cut(s) 1664
Asp700I GAANNNNTTC 1 cut(s) 211
Asp718I GGTACC 1 cut(s) 1045
AspLEI GCGC 1 cut(s) 2251
AsuC2I CCSGG 5 cut(s) 41, 260, 692, 1454, 1979
AsuHPI GGTGA 9 cut(s) 185, 211, 290, 833, 893, 1092, 1119, 1261, 2125
AsuNHI GCTAGC 1 cut(s) 1477
AvaII GGWCC 9 cut(s) 263, 270, 742, 872, 900, 1808, 2022, 2064, 2295
BamHI GGATCC 2 cut(s) 357, 534
BanI GGYRCC 2 cut(s) 510, 1045
BauI CACGAG 1 cut(s) 153
BbrPI CACGTG 1 cut(s) 1999
BbsI GAAGAC 2 cut(s) 42, 2118
BbvI GCAGC 5 cut(s) 118, 707, 813, 1220, 1885
BciT130I CCWGG 3 cut(s) 437, 525, 846
BclI TGATCA 1 cut(s) 1945
BcnI CCSGG 5 cut(s) 41, 260, 692, 1454, 1979
BcoDI GTCTC 8 cut(s) 192, 659, 1186, 1190, 1513, 1680, 2056, 2182
BfaI CTAG 4 cut(s) 507, 630, 1304, 1478
BfmI CTRYAG 1 cut(s) 2210
BfuAI ACCTGC 1 cut(s) 831
BglII AGATCT 1 cut(s) 1917
BisI GCNGC 5 cut(s) 132, 696, 827, 1209, 1899
BlsI GCNGC 5 cut(s) 133, 697, 828, 1210, 1900
Bme1390I CCNGG 8 cut(s) 41, 260, 437, 525, 692, 846, 1454, 1979
Bme18I GGWCC 9 cut(s) 263, 270, 742, 872, 900, 1808, 2022, 2064, 2295
BmgBI CACGTC 2 cut(s) 551, 969
BmrFI CCNGG 8 cut(s) 41, 260, 437, 525, 692, 846, 1454, 1979
BmrI ACTGGG 4 cut(s) 1070, 1164, 1329, 2185
BmsI GCATC 3 cut(s) 1839, 2096, 2279
BmtI GCTAGC 1 cut(s) 1481
BmuI ACTGGG 4 cut(s) 1070, 1164, 1329, 2185
BoxI GACNNNNGTC 2 cut(s) 240, 261
BpiI GAAGAC 2 cut(s) 42, 2118
BplI GAGNNNNNCTC 2 cut(s) 1757, 1789
BpmI CTGGAG 3 cut(s) 458, 1495, 1781
BpuEI CTTGAG 3 cut(s) 332, 344, 1172
BpuMI CCSGG 5 cut(s) 41, 260, 692, 1454, 1979
BsaAI YACGTR 2 cut(s) 1444, 1999
BsaHI GRCGYC 2 cut(s) 703, 2205
BsaI GGTCTC 1 cut(s) 2056
BsaJI CCNNGG 9 cut(s) 259, 266, 524, 690, 844, 868, 1261, 1670, 1977
BsaWI WCCGGW 2 cut(s) 61, 1228
BsaXI ACNNNNNCTCC 2 cut(s) 1343, 1373
Bse118I RCCGGY 1 cut(s) 73
Bse1I ACTGG 6 cut(s) 1076, 1159, 1324, 1512, 1798, 2191
Bse3DI GCAATG 1 cut(s) 105
BseBI CCWGG 3 cut(s) 437, 525, 846
BseDI CCNNGG 9 cut(s) 259, 266, 524, 690, 844, 868, 1261, 1670, 1977
BseGI GGATG 9 cut(s) 192, 334, 687, 1401, 1596, 1830, 2029, 2087, 2259
BseMI GCAATG 1 cut(s) 105
BseMII CTCAG 3 cut(s) 714, 807, 1928
BseNI ACTGG 6 cut(s) 1076, 1159, 1324, 1512, 1798, 2191
BseRI GAGGAG 2 cut(s) 1087, 2044
BseXI GCAGC 5 cut(s) 118, 707, 813, 1220, 1885
BseYI CCCAGC 3 cut(s) 9, 94, 783
BshFI GGCC 6 cut(s) 77, 230, 307, 372, 849, 2144
BshNI GGYRCC 2 cut(s) 510, 1045
BsiSI CCGG 8 cut(s) 41, 62, 74, 259, 692, 1229, 1454, 1979
BslFI GGGAC 5 cut(s) 317, 673, 755, 1103, 1505
BsmAI GTCTC 8 cut(s) 192, 659, 1186, 1190, 1513, 1680, 2056, 2182
BsmBI CGTCTC 1 cut(s) 1190
BsmFI GGGAC 5 cut(s) 317, 673, 755, 1103, 1505
BsmI GAATGC 1 cut(s) 1625
BsnI GGCC 6 cut(s) 77, 230, 307, 372, 849, 2144
Bso31I GGTCTC 1 cut(s) 2056
BspACI CCGC 2 cut(s) 662, 1562
BspANI GGCC 6 cut(s) 77, 230, 307, 372, 849, 2144
BspCNI CTCAG 3 cut(s) 715, 806, 1927
BspHI TCATGA 1 cut(s) 643
BspMI ACCTGC 1 cut(s) 831
BspOI GCTAGC 1 cut(s) 1481
BspT107I GGYRCC 2 cut(s) 510, 1045
BspTNI GGTCTC 1 cut(s) 2056
BsrBI CCGCTC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 105
BsrFI RCCGGY 1 cut(s) 73
BsrI ACTGG 6 cut(s) 1076, 1159, 1324, 1512, 1798, 2191
BssAI RCCGGY 1 cut(s) 73
BssECI CCNNGG 9 cut(s) 259, 266, 524, 690, 844, 868, 1261, 1670, 1977
BssNI GRCGYC 2 cut(s) 703, 2205
BssSI CACGAG 1 cut(s) 153
BssT1I CCWWGG 3 cut(s) 266, 868, 1670
Bst2BI CACGAG 1 cut(s) 153
Bst2UI CCWGG 3 cut(s) 437, 525, 846
Bst4CI ACNGT 6 cut(s) 289, 433, 467, 626, 1117, 2214
Bst6I CTCTTC 1 cut(s) 1197
BstACI GRCGYC 2 cut(s) 703, 2205
BstBAI YACGTR 2 cut(s) 1444, 1999
BstC8I GCNNGC 2 cut(s) 1479, 1526
BstDEI CTNAG 3 cut(s) 723, 793, 1914
BstDSI CCRYGG 1 cut(s) 1261
BstEII GGTNACC 1 cut(s) 1267
BstF5I GGATG 9 cut(s) 192, 334, 687, 1401, 1596, 1830, 2029, 2087, 2259
BstHHI GCGC 1 cut(s) 2251
BstMAI GTCTC 8 cut(s) 192, 659, 1186, 1190, 1513, 1680, 2056, 2182
BstMWI GCNNNNNNNGC 2 cut(s) 10, 1205
BstNI CCWGG 3 cut(s) 437, 525, 846
BstPAI GACNNNNGTC 2 cut(s) 240, 261
BstPI GGTNACC 1 cut(s) 1267
BstSCI CCNGG 8 cut(s) 39, 258, 435, 523, 690, 844, 1452, 1977
BstSFI CTRYAG 1 cut(s) 2210
BstV1I GCAGC 5 cut(s) 118, 707, 813, 1220, 1885
BstV2I GAAGAC 2 cut(s) 42, 2118
BstX2I RGATCY 6 cut(s) 144, 357, 534, 1250, 1917, 1940
BstXI CCANNNNNNTGG 3 cut(s) 368, 413, 561
BstYI RGATCY 6 cut(s) 144, 357, 534, 1250, 1917, 1940
BsuRI GGCC 6 cut(s) 77, 230, 307, 372, 849, 2144
BtgI CCRYGG 1 cut(s) 1261
BtrI CACGTC 2 cut(s) 551, 969
BtsCI GGATG 9 cut(s) 192, 334, 687, 1401, 1596, 1830, 2029, 2087, 2259
BtsI GCAGTG 1 cut(s) 1474
BtsIMutI CAGTG 5 cut(s) 631, 1113, 1152, 1474, 1779
BveI ACCTGC 1 cut(s) 831
Cac8I GCNNGC 2 cut(s) 1479, 1526
CciI TCATGA 1 cut(s) 643
CfoI GCGC 1 cut(s) 2251
Cfr10I RCCGGY 1 cut(s) 73
CseI GACGC 1 cut(s) 711
Csp6I GTAC 3 cut(s) 429, 1046, 1550
CviQI GTAC 3 cut(s) 429, 1046, 1550
DdeI CTNAG 3 cut(s) 723, 793, 1914
DrdI GACNNNNNNGTC 1 cut(s) 1499
DseDI GACNNNNNNGTC 1 cut(s) 1499
Eam1104I CTCTTC 1 cut(s) 1197
EarI CTCTTC 1 cut(s) 1197
Eco130I CCWWGG 3 cut(s) 266, 868, 1670
Eco31I GGTCTC 1 cut(s) 2056
Eco47I GGWCC 9 cut(s) 263, 270, 742, 872, 900, 1808, 2022, 2064, 2295
Eco57I CTGAAG 1 cut(s) 1221
Eco72I CACGTG 1 cut(s) 1999
Eco91I GGTNACC 1 cut(s) 1267
EcoO109I RGGNCCY 3 cut(s) 263, 742, 900
EcoO65I GGTNACC 1 cut(s) 1267
EcoRII CCWGG 3 cut(s) 435, 523, 844
EcoT14I CCWWGG 3 cut(s) 266, 868, 1670
EcoT22I ATGCAT 1 cut(s) 1627
ErhI CCWWGG 3 cut(s) 266, 868, 1670
Esp3I CGTCTC 1 cut(s) 1190
FaqI GGGAC 5 cut(s) 317, 673, 755, 1103, 1505
FbaI TGATCA 1 cut(s) 1945
FblI GTMKAC 1 cut(s) 1070
Fnu4HI GCNGC 5 cut(s) 132, 696, 827, 1209, 1899
FokI GGATG 9 cut(s) 179, 341, 694, 1388, 1603, 1817, 2016, 2074, 2246
Fsp4HI GCNGC 5 cut(s) 132, 696, 827, 1209, 1899
FspBI CTAG 4 cut(s) 507, 630, 1304, 1478
FspI TGCGCA 1 cut(s) 2250
GlaI GCGC 1 cut(s) 2250
GluI GCNGC 5 cut(s) 132, 696, 827, 1209, 1899
GsaI CCCAGC 3 cut(s) 13, 98, 787
GsuI CTGGAG 3 cut(s) 458, 1495, 1781
HaeIII GGCC 6 cut(s) 77, 230, 307, 372, 849, 2144
HapII CCGG 8 cut(s) 41, 62, 74, 259, 692, 1229, 1454, 1979
HgaI GACGC 1 cut(s) 711
HhaI GCGC 1 cut(s) 2251
Hin1I GRCGYC 2 cut(s) 703, 2205
Hin6I GCGC 1 cut(s) 2249
HinP1I GCGC 1 cut(s) 2249
HincII GTYRAC 5 cut(s) 245, 463, 614, 1450, 2236
HindII GTYRAC 5 cut(s) 245, 463, 614, 1450, 2236
HpaII CCGG 8 cut(s) 41, 62, 74, 259, 692, 1229, 1454, 1979
HphI GGTGA 9 cut(s) 185, 211, 290, 833, 893, 1092, 1119, 1261, 2125
Hpy99I CGWCG 2 cut(s) 705, 1168
HpyAV CCTTC 7 cut(s) 419, 729, 861, 885, 891, 1013, 1190
HpyCH4III ACNGT 6 cut(s) 289, 433, 467, 626, 1117, 2214
HpyCH4IV ACGT 9 cut(s) 550, 754, 968, 1166, 1336, 1348, 1443, 1998, 2205
HpyCH4V TGCA 8 cut(s) 4, 419, 639, 829, 1625, 1830, 2087, 2168
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 1205
HpyF3I CTNAG 3 cut(s) 723, 793, 1914
HpySE526I ACGT 9 cut(s) 550, 754, 968, 1166, 1336, 1348, 1443, 1998, 2205
Hsp92I GRCGYC 2 cut(s) 703, 2205
HspAI GCGC 1 cut(s) 2249
KpnI GGTACC 1 cut(s) 1049
Ksp22I TGATCA 1 cut(s) 1945
LmnI GCTCC 3 cut(s) 381, 1003, 1713
Lsp1109I GCAGC 5 cut(s) 118, 707, 813, 1220, 1885
LweI GCATC 3 cut(s) 1839, 2096, 2279
MaeI CTAG 4 cut(s) 507, 630, 1304, 1478
MaeII ACGT 9 cut(s) 550, 754, 968, 1166, 1336, 1348, 1443, 1998, 2205
MaeIII GTNAC 7 cut(s) 281, 620, 712, 811, 969, 1267, 1349
MbiI CCGCTC 1 cut(s) 662
MboII GAAGA 7 cut(s) 47, 846, 904, 1214, 1645, 2114, 2118
MflI RGATCY 6 cut(s) 144, 357, 534, 1250, 1917, 1940
MluCI AATT 9 cut(s) 116, 604, 1039, 1291, 1426, 1665, 1698, 1885, 2059
MlyI GAGTC 3 cut(s) 497, 1559, 2264
MmeI TCCRAC 5 cut(s) 400, 449, 1076, 1858, 2099
Mph1103I ATGCAT 1 cut(s) 1627
MroXI GAANNNNTTC 1 cut(s) 211
MseI TTAA 5 cut(s) 1106, 1233, 1344, 1664, 1854
MspI CCGG 8 cut(s) 41, 62, 74, 259, 692, 1229, 1454, 1979
MspR9I CCNGG 8 cut(s) 41, 260, 437, 525, 692, 846, 1454, 1979
Mva1269I GAATGC 1 cut(s) 1625
MvaI CCWGG 3 cut(s) 437, 525, 846
MwoI GCNNNNNNNGC 2 cut(s) 10, 1205
NciI CCSGG 5 cut(s) 41, 260, 692, 1454, 1979
NheI GCTAGC 1 cut(s) 1477
NmuCI GTSAC 5 cut(s) 281, 620, 811, 969, 1267
NsbI TGCGCA 1 cut(s) 2250
NsiI ATGCAT 1 cut(s) 1627
PagI TCATGA 1 cut(s) 643
PaqCI CACCTGC 1 cut(s) 831
PctI GAATGC 1 cut(s) 1625
PdmI GAANNNNTTC 1 cut(s) 211
PfeI GAWTC 7 cut(s) 188, 769, 1394, 1845, 1967, 2113, 2197
PflFI GACNNNGTC 1 cut(s) 41
PflMI CCANNNNNTGG 1 cut(s) 1042
PfoI TCCNGGA 1 cut(s) 435
PkrI GCNGC 5 cut(s) 133, 697, 828, 1210, 1900
PleI GAGTC 3 cut(s) 497, 1559, 2263
PmaCI CACGTG 1 cut(s) 1999
PmlI CACGTG 1 cut(s) 1999
PpsI GAGTC 3 cut(s) 497, 1559, 2263
Ppu21I YACGTR 2 cut(s) 1444, 1999
PpuMI RGGWCCY 3 cut(s) 263, 742, 900
PshAI GACNNNNGTC 2 cut(s) 240, 261
PshBI ATTAAT 1 cut(s) 1664
Psp1406I AACGTT 1 cut(s) 1336
Psp5II RGGWCCY 3 cut(s) 263, 742, 900
Psp6I CCWGG 3 cut(s) 435, 523, 844
PspCI CACGTG 1 cut(s) 1999
PspEI GGTNACC 1 cut(s) 1267
PspFI CCCAGC 3 cut(s) 9, 94, 783
PspGI CCWGG 3 cut(s) 435, 523, 844
PspPPI RGGWCCY 3 cut(s) 263, 742, 900
PsuI RGATCY 6 cut(s) 144, 357, 534, 1250, 1917, 1940
PsyI GACNNNGTC 1 cut(s) 41
RsaI GTAC 3 cut(s) 430, 1047, 1551
RsaNI GTAC 3 cut(s) 429, 1046, 1550
SaqAI TTAA 5 cut(s) 1106, 1233, 1344, 1664, 1854
SatI GCNGC 5 cut(s) 132, 696, 827, 1209, 1899
SchI GAGTC 3 cut(s) 497, 1559, 2264
ScrFI CCNGG 8 cut(s) 41, 260, 437, 525, 692, 846, 1454, 1979
SfaNI GCATC 3 cut(s) 1839, 2096, 2279
SfcI CTRYAG 1 cut(s) 2210
SinI GGWCC 9 cut(s) 263, 270, 742, 872, 900, 1808, 2022, 2064, 2295
SmlI CTYRAG 3 cut(s) 311, 323, 1187
SmoI CTYRAG 3 cut(s) 311, 323, 1187
Sse9I AATT 9 cut(s) 116, 604, 1039, 1291, 1426, 1665, 1698, 1885, 2059
SsiI CCGC 2 cut(s) 662, 1562
SspMI CTAG 4 cut(s) 507, 630, 1304, 1478
StyD4I CCNGG 8 cut(s) 39, 258, 435, 523, 690, 844, 1452, 1977
StyI CCWWGG 3 cut(s) 266, 868, 1670
TaaI ACNGT 6 cut(s) 289, 433, 467, 626, 1117, 2214
TaiI ACGT 9 cut(s) 553, 757, 971, 1169, 1339, 1351, 1446, 2001, 2208
TaqI TCGA 6 cut(s) 319, 501, 700, 772, 915, 1965
TasI AATT 9 cut(s) 116, 604, 1039, 1291, 1426, 1665, 1698, 1885, 2059
TatI WGTACW 1 cut(s) 1549
TfiI GAWTC 7 cut(s) 188, 769, 1394, 1845, 1967, 2113, 2197
Tru1I TTAA 5 cut(s) 1106, 1233, 1344, 1664, 1854
Tru9I TTAA 5 cut(s) 1106, 1233, 1344, 1664, 1854
TscAI CASTG 5 cut(s) 631, 1120, 1159, 1474, 1786
TseFI GTSAC 5 cut(s) 281, 620, 811, 969, 1267
TseI GCWGC 5 cut(s) 131, 695, 826, 1208, 1898
Tsp45I GTSAC 5 cut(s) 281, 620, 811, 969, 1267
TspDTI ATGAA 6 cut(s) 91, 594, 660, 925, 995, 1710
TspGWI ACGGA 5 cut(s) 262, 394, 473, 527, 2252
TspRI CASTG 5 cut(s) 631, 1120, 1159, 1474, 1786
Tth111I GACNNNGTC 1 cut(s) 41
Van91I CCANNNNNTGG 1 cut(s) 1042
VpaK11BI GGWCC 9 cut(s) 263, 270, 742, 872, 900, 1808, 2022, 2064, 2295
VspI ATTAAT 1 cut(s) 1664
XapI RAATTY 3 cut(s) 116, 1039, 1426
XcmI CCANNNNNNNNNTGG 2 cut(s) 223, 1818
XmiI GTMKAC 1 cut(s) 1070
XmnI GAANNNNTTC 1 cut(s) 211
XspI CTAG 4 cut(s) 507, 630, 1304, 1478
ZraI GACGTC 1 cut(s) 2206
Zsp2I ATGCAT 1 cut(s) 1627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.