RchiOBHm_Chr1g0344541

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000147
Physical Location & Seq
Reverse (-)
36892926 .. 36899020
6095 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57098

Sequence Viewer

Length: 4098 bp
ATGAGGTTCCTTGCTTGGAATTGTCAGGGACTTGGGTCATCTCTGACATCAAAGGCTCTGCGCAGACTATGGCGCAAGTATGACCCAGATATGCTGTTTTTGATGGAAACTCATCAACCCTCTGATGTGATTTCCACTTGGCATAGGCAGCTAAAGTTTGTTAACTCTGTCACTGTTAACCCTATAAATACTAGTAGTGGTGGTCTGGCTCTGTTTTGGAAGTCTTCAGTTCAAGTTTCAGTTGTGTTTGAGTCGCAGAATGTGATAGATACTGATGTTTTGTTTGTAAATGAAGACTTTCGTTGTAAGATCAGCTGGATGTACGGTAATCCTTTTGACAATCAAAAAAATATGTTTTGGAACTATATGATTCGTCGTTTCTCTTTTCAGACTCTGCCATGGTTTTGTTTAGGGGATTTTAATGAGATTCAGTGGCCTTTTGAGAAATGGGGCGGTGTTCCTCATCCTCAATGGCGTATGAATCTTTTCAATCAGTTCATTGACCATGCTGGTCTCAGAGATTTACACTTTCAGGGCCCCTGTTTTACTTGGCATCGTTATCGTTTTGGTCAACTTTATCTAAAGGAGCGCTTGGACAGGGGTCTTGGAAACTCAAGCTGGTGTTTGTCTCAACCAAAAACCCAAATATTTCATCTTCCAATGGTGGGGTCGGATCATAGACCTCTGATTTTGGATACGGCGCCTCCAGAATCCCCCTCTCCAAAGCTCTTTAGGTTGGAGCATATTTGGACAACAAGTGCGGACTGTGGTCAACTAATTTCTCAACATTGGGTGGATGCTTCCGGTTCAGAGGCAATGCCTACTTGGATACAGAATTTGAAGGAGTGTAAACGCTCACTTTTAGCCTGGTGCAAGCAAGCTTTTCCGAACTCTCATTTGTTGGTGCAAGAGCTATTGGTTCAGTTAAACCATCTTCATAATAGCAATTCTCCTAATATTGCTCATCAGATTACAACGGTTACTGCTCGAATTGAGGAATTATGGACTTTGGAAGAATTATATTGGCATCAACGGTCGAGGGTTTCTTGGTTGCAACTTGGTGATAAAAACACTTCCTTCTTCCATCATTCTGCTCTTCAACGTCGACAACACAACCGTATTCTTAGACTTCGTAATTCTGATGGCGTTTGGTTGGACTCGGACTTGGCAATTGCTGCAGTTTTCTCCTCTTATTACCAGAAGCTCTTCACTTCAGTTGGTCCGAGAAACTGGGATGATGTTCTCCACCTGGTTGATCCGATTGTGATAGCAGAGATGAATGCTAGTCTTTTAGCTGTAGTAAGTATGGAGGAGGTCAAATCTGCAATTTTTCAGTTGGGGGCTCTTAAGTTCCCGGGTCCGGATGGTTACTCTGGCATTTTTTATCAATCTTATTGGGATACTGTTAAGCATGTTATCCTTGAAGCTACTTCTTCTTTTGTTCAAACTGGTGATCTACTCCCGGAGCTTAACAAGACTTATATTGCCCTTATTCCAAAAGTGCAGGCTCCTGAGCATGCGTCTCAATTTCGTCCCATTGGCCTCTGTAACTTTGCTTACAAAGTCCTTTCCAAAGTTTTAGCCAACCGCTTGAAACCCTATATGCCTACGCTGATCTCTGAAAATCAGGCGGCTTTTGTAGCAGGTCGTCAAATTCAAGATAATGTGGTCATTGCACATGAAATGTTCCACCATATTAAACTTCTTCGAAATGTTGGAGATGAAGTCTTTGGTTTGAAATTGGATATCAATAAGGCTTACGATTGCGTAGAATGGGATTTTTTAGAGGCGGTGTTGCTTAAACTTGGATTTGCAGATCATTGGGTTCTTCTGATCATGAGGTGCATTCGTTCTGTGTCTCTGTCTACTATGGTGAATGGTAAACCTTCAGCATTCTTTACTCCTACTCGGGGAATTCGCCAGGGTGATCCTCTATCTCCATATCTCTTCCTTTTTGTCACAGACGTGTTGTCTACTATGCTTACAAAGGCCTGTAGCAATCAGTGGTTGCGTCCTCTTCATTTAACTGTCCCTGGTCCAATTATCAGCCATTTATTTTTTGCGGATGACTCTCTGCTTTTCTTCCAAGCTTCTCGACAAAATGCTATGGCCCTGGTTTTTTTATTGAAATCGTATAGTACTGCCTCAGGTCAACAAATCAACTATGACAAATCTATGCTCTACTTTAGTCCTAATACTCATCCTGCTTTGATTGTGTCTATTACTTCTATTCTAAGAGTTCATTCTACGGATAATCCGGGCTCCTATTTAGGTTTGCCTACTGTTTGGGGCAGATCGAAGCGTGCAGCTTTGGGTTATGTTCGAGATGCTGTTCTTAGGAAAATTCAAGGTTGGAAACAAGCTTCATTATCTCCGGCAGGAAAGGAAATCATGATAAAGGCGGTGGCTATGGCACTTCCTGCCTATCCAATGTCAATTTTCAAGTTTCCAAAATCCCTTTGTGTTCAGATTAATAATGCTTTGGCAAAGTTTTGGTGGGGTACAAATGGTGACAAGGGTATTCACTGGAAGCATTGGGATCTTTTGTGCAATCCGAAATCTGTGGGAGGTCTTGGTTTTCGTAGCTTACAGGACTTCAATTTAGCTTTGTTAGCTAAGCAGGGTTGGCGTCTGCTTCAAAATCCTTCTGCAAAATGGGCTCAGGTCCTGAAATATAGGTATTTCCCAAATACCTCCTTCATGGAGGCCAAACAGGGTCCTTCATCTTCCTGGGTGTGGGCGAGTCTGTTGGCTGGTAGAGATGTGTTATTTAAAGGCTCATTATGGAGAGTGGGTAATGGTCACTCTATACATTTATGGAATGACAAATGGATCCCAAACACTCCCAGCTTCAGTACTAGGTTCCCTTCTCTCCTGCCTGTCTCACAATCTGTAAACACCCTTTTAGATTTCTCTACCCTCACCTGGAATTTGGAGTCCCTTCAACATGTGCTCACTCCGGGTGATTCTTCTGTGATTCAGGCCATTCCTATTTTCAGTGAGTTGGTTGATGATAAACTCGTGTGGCCTCATGTTACGAATGGTGAGTATTCGGTGAGGTCGGGCTATCATGTCTCTTTTAACTCAAATATTATTGCTTCAGCTCCATCTCCACATTCCTCTCATGTAGTCCCTCGATCAGTTTGGACTTTGGTTTGGACTATTCGGAGTCTTCCAAAAATAAGGATTTTCCTATGGAGAGTTGTGTCTAATGTGTTAGCAACCAAACAGAATTTGTTTAGAAGACACCTGGGGCGCTCACCTCTCTGTCCTTTTTGCTCAGAATTCCCAGAATCAGTGGAACATCTTCTTCTCTCTTGTCCTTGGGTTGTGCAAACCTGGTTTTTGCACCCACTGGGATATCGGATTCCCTTGCAATCCCTTACTTCTTTTGATCAGTGGTTAAGTACTCTACTTATTCCTCTTCATTCTGAGTTGTCAAGATCTGTGGTGCTTACTCATATCAGCTTTCTTCTTTGGCACATTTGGAAGCATCGGTGTGATTGCATTTTCAATTACAAGGTTCCTAATCCGACTTTGGTGGCTACAAGGGCTTTTGTGGCTTCTTCAGAGTTTTTAACATCTCGTACTCTGTCTCTTACTTGCAATAGGCCTCCCCCTCCTCCCCCTCCTCCTCCTCCACCTTGGACTCCTCCTTGTTTAGATTTTGTTAAGATAAATACAGATGCTTCTTGGGATAAACAATCTCTGCAGGCTGGTTTGGCAGTGATAGTTCGAAATAGTTCTGGTGAGCTCCTTCATGAAACTTTGGCCCTTTATCAAGCCGTGTTGCTGGCCTTATGTTTAAATTTTCAAAAAGTTATTTTTGAAATGGATTCCTTTCATCTTGTGCATCTCCTGAATGATTCCCACTTGCTACCGGATTGGATCATATTTCCGCTGGTGCATTATATCCGCGAGATCACAGTTGTTATTCCTTACGTTAGCTGGGTTTGGACCAGCAGGAACGCCAATCAAGCTGCTGATCATATGGCTTCTTTGTCTCGCAGGAGGATGGGCCCTTCTGATTGGGTTAATCATCCTCCTTCTACCTTCACCCGTATTCTCCTTTATGATGCTGGACATGCTCCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

1365

Amino Acids

155.95

Weight (kDa)

9.18

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 5 - 226 2.7e-11 Endonuclease/Exonuclease/phosphatase family
RVT_1 PF00078 500 - 759 1.1e-28 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 1017 - 1114 1.6e-15 zinc-binding in reverse transcriptase
RVT_3 PF13456 1227 - 1333 6.7e-10 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000147)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10354 FvH4_1g17131 FvH4_2g11611 FvH4_2g33851 FvH4_3g32112 FvH4_3g35052 FvH4_3g44611 FvH4_4g05921 FvH4_4g06411 FvH4_4g06792 FvH4_4g10365 FvH4_4g21422 FvH4_4g22401 FvH4_4g30261 FvH4_5g08843 FvH4_5g14731 FvH4_5g24781 FvH4_5g26231 FvH4_5g31282 FvH4_6g23371 FvH4_6g38511 FvH4_7g01171 FvH4_7g03341 FvH4_7g06171 FvH4_7g32472
prunus_persica Prupe.2G036900_v2.0.a1
pyrus_communis pycom02g19900 pycom03g01520 pycom05g10510 pycom16g23820
rosa_chinensis RchiOBHm_Chr1g0316031 RchiOBHm_Chr1g0342451 RchiOBHm_Chr1g0344541 RchiOBHm_Chr1g0364721 RchiOBHm_Chr2g0133541 RchiOBHm_Chr2g0134511 RchiOBHm_Chr2g0145891 RchiOBHm_Chr2g0163241 RchiOBHm_Chr5g0027201 RchiOBHm_Chr5g0027881 RchiOBHm_Chr5g0047471 RchiOBHm_Chr7g0210901
rosa_laevigata RLG00000011116 RLG00000017964 RLG00000018128 RLG00000018387 RLG00000018829 RLG00000019353 RLG00000028973 RLG00000035870 RLG00000036629
rosa_multiflora Rmu_co8126340.1_g000001 Rmu_co8216750.1_g000001 Rmu_co8222072.1_g000001 Rmu_co8332453.1_g000001 Rmu_co8349913.1_g000001 Rmu_co8405337.1_g000001 Rmu_sc0000041.1_g000012 Rmu_sc0000166.1_g000029 Rmu_sc0000251.1_g000019 Rmu_sc0000271.1_g000028 Rmu_sc0000509.1_g000036 Rmu_sc0000543.1_g000048 Rmu_sc0000808.1_g000003 Rmu_sc0000847.1_g000040 Rmu_sc0000972.1_g000020 Rmu_sc0001260.1_g000001 Rmu_sc0001464.1_g000026 Rmu_sc0001616.1_g000003 Rmu_sc0001669.1_g000001 Rmu_sc0002005.1_g000001 Rmu_sc0002014.1_g000010 Rmu_sc0002082.1_g000033 Rmu_sc0002302.1_g000024 Rmu_sc0002531.1_g000045 Rmu_sc0003276.1_g000002 Rmu_sc0003737.1_g000007 Rmu_sc0003832.1_g000022 Rmu_sc0004080.1_g000011 Rmu_sc0004487.1_g000009 Rmu_sc0004988.1_g000018 Rmu_sc0004988.1_g000019 Rmu_sc0005254.1_g000002 Rmu_sc0005733.1_g000004 Rmu_sc0006413.1_g000006 Rmu_sc0007121.1_g000013 Rmu_sc0007869.1_g000006 Rmu_sc0008035.1_g000015 Rmu_sc0008279.1_g000017 Rmu_sc0008351.1_g000014 Rmu_sc0008957.1_g000007 Rmu_sc0009012.1_g000007 Rmu_sc0010556.1_g000009 Rmu_sc0012889.1_g000003 Rmu_sc0019808.1_g000004 Rmu_sc0028113.1_g000001 Rmu_sc0031202.1_g000001 Rmu_sc0034436.1_g000001 Rmu_sc0034445.1_g000001 Rmu_sc0040044.1_g000001 Rmu_ssc0000062.1_g000032 Rmu_ssc0000372.1_g000018
rosa_roxburghii Rroxscaffold_1G00001520 Rroxscaffold_1G00039990 Rroxscaffold_1G00046370 Rroxscaffold_2G00110140 Rroxscaffold_2G00131420 Rroxscaffold_3G00222390 Rroxscaffold_5G00336040 Rroxscaffold_5G00338000 Rroxscaffold_5G00344030 Rroxscaffold_5G00363460 Rroxscaffold_6G00402000 Rroxscaffold_7G00194940
rosa_rugosa Rorug01G0106500 Rorug01G0111300 Rorug01G0247000 Rorug02G0318100.1 Rorug02G0318200 Rorug02G0459500 Rorug03G0108100 Rorug03G0200500 Rorug03G0232500 Rorug03G0240600 Rorug04G0105300 Rorug04G0256600 Rorug06G0079300 Rorug06G0082700 Rorug06G0095600 Rorug06G0179000 Rorug07G0113500 Rorug07G0271100
rosa_samantha Rh1AG087200 Rh1CG085300 Rh1DG013200 Rh1DG091700 Rh1DG091800 Rh1DG338300 Rh2AG375400 Rh2AG407000 Rh2AG513500 Rh2BG277700 Rh2BG371200 Rh2BG376100 Rh2BG580500 Rh2CG354300 Rh2DG200100 Rh2DG388000 Rh2DG393500 Rh2DG426400 Rh3CG304800 Rh4AG044600 Rh4AG220500 Rh4BG379300 Rh4CG007100 Rh4CG030300 Rh4CG038000 Rh4CG114900 Rh4CG393700 Rh5AG124900 Rh5AG342300 Rh5BG414400 Rh5CG263800 Rh5CG439000 Rh5DG231100 Rh5DG566900 Rh6AG138600 Rh6AG200000 Rh6AG253100 Rh6AG390900 Rh6AG441600 Rh6BG194700 Rh6BG225800 Rh6BG256100 Rh6CG231700 Rh6CG231900 Rh6CG312200 Rh6CG453700 Rh6DG219400 Rh7AG357700 Rh7AG442000 Rh7CG425700 Rh7CG474600 Rh7CG494100 Rh7DG253900 Rh7DG294700 Rh7DG401600 Rh7DG461300
rosa_wichuraiana Rw1G001190 Rw2G047020 Rw4G003490 Rw5G039690 Rw6G010450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 62
Acc36I ACCTGC 1 cut(s) 1634
AccB1I GGYRCC 1 cut(s) 700
AccI GTMKAC 3 cut(s) 1105, 1865, 1973
AccII CGCG 1 cut(s) 3918
AccIII TCCGGA 1 cut(s) 1360
AciI CCGC 9 cut(s) 453, 761, 1588, 1631, 1790, 2063, 2402, 3899, 3916
AclWI GGATC 7 cut(s) 681, 1250, 1922, 2547, 2827, 2840, 3896
AcsI RAATTY 8 cut(s) 835, 1653, 1914, 2343, 2929, 3232, 3284, 3808
AcuI CTGAAG 6 cut(s) 210, 1197, 1872, 2836, 3084, 3552
AcyI GRCGYC 2 cut(s) 701, 2629
AfaI GTAC 6 cut(s) 323, 2140, 2503, 2857, 3409, 3589
AfeI AGCGCT 1 cut(s) 590
AfiI CCNNNNNNNGG 5 cut(s) 665, 1360, 1910, 2736, 2925
AflII CTTAAG 1 cut(s) 1346
AflIII ACRYGT 2 cut(s) 1965, 2947
AhdI GACNNNNNGTC 1 cut(s) 1969
AhlI ACTAGT 1 cut(s) 191
AjiI CACGTC 1 cut(s) 1966
AjnI CCWGG 9 cut(s) 866, 1248, 1920, 2032, 2112, 2729, 2924, 3249, 3338
AleI CACNNNNGTG 1 cut(s) 1964
Alw21I GWGCWC 2 cut(s) 2955, 3756
Alw26I GTCTC 8 cut(s) 518, 633, 1527, 1863, 2887, 3079, 3600, 4008
AlwI GGATC 7 cut(s) 681, 1250, 1922, 2547, 2827, 2840, 3896
AlwNI CAGNNNCTG 2 cut(s) 394, 2668
Ama87I CYCGRG 2 cut(s) 1354, 1908
Aor13HI TCCGGA 1 cut(s) 1360
Aor51HI AGCGCT 1 cut(s) 590
ApaI GGGCCC 2 cut(s) 539, 4022
ApeKI GCWGC 4 cut(s) 148, 1175, 2306, 3980
ApoI RAATTY 8 cut(s) 835, 1653, 1914, 2343, 2929, 3232, 3284, 3808
AseI ATTAAT 1 cut(s) 2472
Asp700I GAANNNNTTC 5 cut(s) 297, 485, 1205, 3306, 3742
AspLEI GCGC 5 cut(s) 63, 75, 591, 703, 3258
AsuC2I CCSGG 5 cut(s) 1355, 1356, 1463, 2259, 2961
AsuII TTCGAA 2 cut(s) 1708, 3736
AvaI CYCGRG 2 cut(s) 1354, 1908
AvaII GGWCC 6 cut(s) 1220, 1358, 2036, 2665, 2717, 3957
AxyI CCTNAGG 1 cut(s) 2146
BaeGI GKGCMC 2 cut(s) 539, 4022
BaeI ACNNNNGTAYC 2 cut(s) 261, 294
BamHI GGATCC 1 cut(s) 2832
BanI GGYRCC 1 cut(s) 700
BanII GRGCYC 6 cut(s) 539, 1345, 2264, 2662, 3756, 4022
BauI CACGAG 1 cut(s) 3020
BbsI GAAGAC 4 cut(s) 216, 300, 3164, 3250
Bbv12I GWGCWC 2 cut(s) 2955, 3756
BbvI GCAGC 4 cut(s) 160, 1162, 2318, 3967
BccI CCATC 7 cut(s) 97, 939, 1092, 1136, 1358, 3115, 4009
BceAI ACGGC 2 cut(s) 714, 3770
BcgI CGANNNNNNTGC 2 cut(s) 542, 576
BciT130I CCWGG 9 cut(s) 868, 1250, 1922, 2034, 2114, 2731, 2926, 3251, 3340
BciVI GTATCC 3 cut(s) 688, 822, 1393
BclI TGATCA 3 cut(s) 1833, 3394, 3985
BcnI CCSGG 5 cut(s) 1355, 1356, 1463, 2259, 2961
BcoDI GTCTC 8 cut(s) 518, 633, 1527, 1863, 2887, 3079, 3600, 4008
BcuI ACTAGT 1 cut(s) 191
BfaI CTAG 3 cut(s) 192, 1284, 2859
BfmI CTRYAG 4 cut(s) 1176, 1296, 1993, 3710
BfoI RGCGCY 3 cut(s) 592, 704, 3259
BfrI CTTAAG 1 cut(s) 1346
BfuAI ACCTGC 1 cut(s) 1634
BfuI GTATCC 3 cut(s) 688, 822, 1393
BglII AGATCT 1 cut(s) 3443
BisI GCNGC 5 cut(s) 149, 1176, 1632, 2307, 3981
BlpI GCTNAGC 1 cut(s) 2616
BlsI GCNGC 5 cut(s) 150, 1177, 1633, 2308, 3982
BmcAI AGTACT 3 cut(s) 2140, 2857, 3409
Bme18I GGWCC 6 cut(s) 1220, 1358, 2036, 2665, 2717, 3957
BmeRI GACNNNNNGTC 1 cut(s) 1969
BmeT110I CYCGRG 2 cut(s) 1354, 1908
BmgBI CACGTC 1 cut(s) 1966
BmrI ACTGGG 2 cut(s) 1240, 3365
BmsI GCATC 8 cut(s) 562, 787, 1036, 2317, 3502, 3676, 3862, 4066
BmuI ACTGGG 2 cut(s) 1240, 3365
BoxI GACNNNNGTC 3 cut(s) 34, 600, 768
BpiI GAAGAC 4 cut(s) 216, 300, 3164, 3250
BpmI CTGGAG 1 cut(s) 690
Bpu10I CCTNAGC 2 cut(s) 1512, 2661
Bpu1102I GCTNAGC 1 cut(s) 2616
Bpu14I TTCGAA 2 cut(s) 1708, 3736
BpuEI CTTGAG 1 cut(s) 598
BpuMI CCSGG 5 cut(s) 1355, 1356, 1463, 2259, 2961
BsaHI GRCGYC 2 cut(s) 701, 2629
BsaI GGTCTC 1 cut(s) 518
BsaJI CCNNGG 9 cut(s) 398, 1354, 1921, 2032, 2112, 2730, 3250, 3323, 3644
BsaWI WCCGGW 3 cut(s) 803, 1360, 3880
BsaXI ACNNNNNCTCC 6 cut(s) 688, 718, 1710, 1740, 2779, 2809
Bsc4I CCNNNNNNNGG 5 cut(s) 665, 1360, 1910, 2736, 2925
Bse1I ACTGG 4 cut(s) 1235, 1453, 2531, 3360
Bse21I CCTNAGG 1 cut(s) 2146
Bse3DI GCAATG 2 cut(s) 822, 1671
BseAI TCCGGA 1 cut(s) 1360
BseBI CCWGG 9 cut(s) 868, 1250, 1922, 2034, 2114, 2731, 2926, 3251, 3340
BseDI CCNNGG 9 cut(s) 398, 1354, 1921, 2032, 2112, 2730, 3250, 3323, 3644
BseGI GGATG 9 cut(s) 324, 463, 802, 1240, 1369, 2071, 2200, 4020, 4039
BseLI CCNNNNNNNGG 5 cut(s) 665, 1360, 1910, 2736, 2925
BseMI GCAATG 2 cut(s) 822, 1671
BseMII CTCAG 6 cut(s) 529, 1503, 2160, 2675, 3294, 3423
BseNI ACTGG 4 cut(s) 1235, 1453, 2531, 3360
BseRI GAGGAG 7 cut(s) 1177, 1325, 3612, 3621, 3624, 3627, 3642
BseSI GKGCMC 2 cut(s) 539, 4022
BseXI GCAGC 4 cut(s) 160, 1162, 2318, 3967
BseYI CCCAGC 2 cut(s) 2846, 3948
BsgI GTGCAG 2 cut(s) 1523, 2325
Bsh1236I CGCG 1 cut(s) 3918
Bsh1285I CGRYCG 1 cut(s) 1037
BshNI GGYRCC 1 cut(s) 700
BsiEI CGRYCG 1 cut(s) 1037
BsiHKAI GWGCWC 2 cut(s) 2955, 3756
BsiHKCI CYCGRG 2 cut(s) 1354, 1908
BsiSI CCGG 8 cut(s) 804, 1355, 1361, 1463, 2258, 2375, 2960, 3881
BslFI GGGAC 5 cut(s) 42, 1518, 2015, 2923, 3116
BslI CCNNNNNNNGG 5 cut(s) 665, 1360, 1910, 2736, 2925
BsmAI GTCTC 8 cut(s) 518, 633, 1527, 1863, 2887, 3079, 3600, 4008
BsmBI CGTCTC 1 cut(s) 1527
BsmFI GGGAC 5 cut(s) 42, 1518, 2015, 2923, 3116
BsmI GAATGC 3 cut(s) 1285, 1845, 1892
Bso31I GGTCTC 1 cut(s) 518
BsoBI CYCGRG 2 cut(s) 1354, 1908
Bsp119I TTCGAA 2 cut(s) 1708, 3736
Bsp120I GGGCCC 2 cut(s) 535, 4018
Bsp1286I GDGCHC 7 cut(s) 539, 1345, 2264, 2662, 2955, 3756, 4022
Bsp13I TCCGGA 1 cut(s) 1360
Bsp1720I GCTNAGC 1 cut(s) 2616
Bsp19I CCATGG 1 cut(s) 398
BspACI CCGC 9 cut(s) 453, 761, 1588, 1631, 1790, 2063, 2402, 3899, 3916
BspCNI CTCAG 6 cut(s) 528, 1504, 2159, 2674, 3293, 3424
BspEI TCCGGA 1 cut(s) 1360
BspFNI CGCG 1 cut(s) 3918
BspHI TCATGA 3 cut(s) 1836, 2391, 3760
BspMAI CTGCAG 2 cut(s) 1180, 3714
BspMI ACCTGC 1 cut(s) 1634
BspPI GGATC 7 cut(s) 681, 1250, 1922, 2547, 2827, 2840, 3896
BspQI GCTCTTC 2 cut(s) 1101, 1211
BspT104I TTCGAA 2 cut(s) 1708, 3736
BspT107I GGYRCC 1 cut(s) 700
BspTI CTTAAG 1 cut(s) 1346
BspTNI GGTCTC 1 cut(s) 518
BsrDI GCAATG 2 cut(s) 822, 1671
BsrI ACTGG 4 cut(s) 1235, 1453, 2531, 3360
BssECI CCNNGG 9 cut(s) 398, 1354, 1921, 2032, 2112, 2730, 3250, 3323, 3644
BssNI GRCGYC 2 cut(s) 701, 2629
BssSI CACGAG 1 cut(s) 3020
BssT1I CCWWGG 3 cut(s) 398, 3323, 3644
Bst2BI CACGAG 1 cut(s) 3020
Bst2UI CCWGG 9 cut(s) 868, 1250, 1922, 2034, 2114, 2731, 2926, 3251, 3340
Bst6I CTCTTC 5 cut(s) 1101, 1211, 1952, 2022, 3429
BstACI GRCGYC 2 cut(s) 701, 2629
BstAFI CTTAAG 1 cut(s) 1346
BstAPI GCANNNNNTGC 2 cut(s) 1175, 2420
BstBI TTCGAA 2 cut(s) 1708, 3736
BstC8I GCNNGC 7 cut(s) 875, 879, 1506, 1518, 2304, 3714, 3795
BstDSI CCRYGG 1 cut(s) 398
BstF5I GGATG 9 cut(s) 324, 463, 802, 1240, 1369, 2071, 2200, 4020, 4039
BstFNI CGCG 1 cut(s) 3918
BstH2I RGCGCY 3 cut(s) 592, 704, 3259
BstHHI GCGC 5 cut(s) 63, 75, 591, 703, 3258
BstMAI GTCTC 8 cut(s) 518, 633, 1527, 1863, 2887, 3079, 3600, 4008
BstMCI CGRYCG 1 cut(s) 1037
BstNI CCWGG 9 cut(s) 868, 1250, 1922, 2034, 2114, 2731, 2926, 3251, 3340
BstNSI RCATGY 4 cut(s) 1415, 1520, 2951, 4088
BstPAI GACNNNNGTC 3 cut(s) 34, 600, 768
BstSFI CTRYAG 4 cut(s) 1176, 1296, 1993, 3710
BstSLI GKGCMC 2 cut(s) 539, 4022
BstUI CGCG 1 cut(s) 3918
BstV1I GCAGC 4 cut(s) 160, 1162, 2318, 3967
BstV2I GAAGAC 4 cut(s) 216, 300, 3164, 3250
BstX2I RGATCY 3 cut(s) 2539, 2832, 3443
BstYI RGATCY 3 cut(s) 2539, 2832, 3443
Bsu36I CCTNAGG 1 cut(s) 2146
BsuI GTATCC 3 cut(s) 688, 822, 1393
BtgI CCRYGG 1 cut(s) 398
BtrI CACGTC 1 cut(s) 1966
BtsCI GGATG 9 cut(s) 324, 463, 802, 1240, 1369, 2071, 2200, 4020, 4039
BtsI GCAGTG 1 cut(s) 3732
BtsIMutI CAGTG 9 cut(s) 171, 437, 2009, 2524, 3004, 3303, 3353, 3404, 3732
BveI ACCTGC 1 cut(s) 1634
Cac8I GCNNGC 7 cut(s) 875, 879, 1506, 1518, 2304, 3714, 3795
CaiI CAGNNNCTG 2 cut(s) 394, 2668
CciI TCATGA 3 cut(s) 1836, 2391, 3760
CfoI GCGC 5 cut(s) 63, 75, 591, 703, 3258
Cfr9I CCCGGG 1 cut(s) 1354
CseI GACGC 3 cut(s) 1509, 2000, 2618
CsiI ACCWGGT 2 cut(s) 1248, 3338
Csp6I GTAC 6 cut(s) 322, 2139, 2502, 2856, 3408, 3588
CspCI CAANNNNNGTGG 2 cut(s) 3429, 3464
CviQI GTAC 6 cut(s) 322, 2139, 2502, 2856, 3408, 3588
DinI GGCGCC 1 cut(s) 702
DraI TTTAAA 2 cut(s) 2773, 3807
DriI GACNNNNNGTC 1 cut(s) 1969
Eam1104I CTCTTC 5 cut(s) 1101, 1211, 1952, 2022, 3429
Eam1105I GACNNNNNGTC 1 cut(s) 1969
EarI CTCTTC 5 cut(s) 1101, 1211, 1952, 2022, 3429
Ecl136II GAGCTC 1 cut(s) 3754
Eco130I CCWWGG 3 cut(s) 398, 3323, 3644
Eco147I AGGCCT 2 cut(s) 1991, 3613
Eco24I GRGCYC 6 cut(s) 539, 1345, 2264, 2662, 3756, 4022
Eco31I GGTCTC 1 cut(s) 518
Eco32I GATATC 2 cut(s) 1747, 3362
Eco47I GGWCC 6 cut(s) 1220, 1358, 2036, 2665, 2717, 3957
Eco47III AGCGCT 1 cut(s) 590
Eco53kI GAGCTC 1 cut(s) 3754
Eco57I CTGAAG 6 cut(s) 210, 1197, 1872, 2836, 3084, 3552
Eco81I CCTNAGG 1 cut(s) 2146
Eco88I CYCGRG 2 cut(s) 1354, 1908
EcoICRI GAGCTC 1 cut(s) 3754
EcoO109I RGGNCCY 5 cut(s) 535, 536, 2665, 2717, 4019
EcoRI GAATTC 2 cut(s) 1914, 3284
EcoRII CCWGG 9 cut(s) 866, 1248, 1920, 2032, 2112, 2729, 2924, 3249, 3338
EcoRV GATATC 2 cut(s) 1747, 3362
EcoT14I CCWWGG 3 cut(s) 398, 3323, 3644
EcoT38I GRGCYC 6 cut(s) 539, 1345, 2264, 2662, 3756, 4022
EgeI GGCGCC 1 cut(s) 702
EheI GGCGCC 1 cut(s) 702
ErhI CCWWGG 3 cut(s) 398, 3323, 3644
Esp3I CGTCTC 1 cut(s) 1527
FalI AAGNNNNNCTT 2 cut(s) 575, 607
FaqI GGGAC 5 cut(s) 42, 1518, 2015, 2923, 3116
FauNDI CATATG 1 cut(s) 3990
FbaI TGATCA 3 cut(s) 1833, 3394, 3985
FblI GTMKAC 3 cut(s) 1105, 1865, 1973
Fnu4HI GCNGC 5 cut(s) 149, 1176, 1632, 2307, 3981
FokI GGATG 9 cut(s) 331, 450, 809, 1247, 1376, 2078, 2187, 4026, 4027
FriOI GRGCYC 6 cut(s) 539, 1345, 2264, 2662, 3756, 4022
Fsp4HI GCNGC 5 cut(s) 149, 1176, 1632, 2307, 3981
FspBI CTAG 3 cut(s) 192, 1284, 2859
FspI TGCGCA 1 cut(s) 62
GlaI GCGC 5 cut(s) 62, 74, 590, 702, 3257
GluI GCNGC 5 cut(s) 149, 1176, 1632, 2307, 3981
GsaI CCCAGC 2 cut(s) 2850, 3952
GsuI CTGGAG 1 cut(s) 690
HaeII RGCGCY 3 cut(s) 592, 704, 3259
HapII CCGG 8 cut(s) 804, 1355, 1361, 1463, 2258, 2375, 2960, 3881
HgaI GACGC 3 cut(s) 1509, 2000, 2618
HhaI GCGC 5 cut(s) 63, 75, 591, 703, 3258
Hin1I GRCGYC 2 cut(s) 701, 2629
Hin6I GCGC 5 cut(s) 61, 73, 589, 701, 3256
HinP1I GCGC 5 cut(s) 61, 73, 589, 701, 3256
HincII GTYRAC 6 cut(s) 163, 178, 572, 773, 1106, 2153
HindII GTYRAC 6 cut(s) 163, 178, 572, 773, 1106, 2153
HindIII AAGCTT 3 cut(s) 879, 2088, 2361
HpaI GTTAAC 2 cut(s) 163, 178
HpaII CCGG 8 cut(s) 804, 1355, 1361, 1463, 2258, 2375, 2960, 3881
Hpy99I CGWCG 2 cut(s) 378, 1107
HpyCH4IV ACGT 3 cut(s) 1102, 1965, 3942
HpySE526I ACGT 3 cut(s) 1102, 1965, 3942
Hsp92I GRCGYC 2 cut(s) 701, 2629
HspAI GCGC 5 cut(s) 61, 73, 589, 701, 3256
KasI GGCGCC 1 cut(s) 700
Kpn2I TCCGGA 1 cut(s) 1360
Ksp22I TGATCA 3 cut(s) 1833, 3394, 3985
KspAI GTTAAC 2 cut(s) 163, 178
LguI GCTCTTC 2 cut(s) 1101, 1211
LmnI GCTCC 8 cut(s) 586, 739, 1465, 1513, 2267, 3109, 3759, 4093
Lsp1109I GCAGC 4 cut(s) 160, 1162, 2318, 3967
LweI GCATC 8 cut(s) 562, 787, 1036, 2317, 3502, 3676, 3862, 4066
MabI ACCWGGT 2 cut(s) 1248, 3338
MaeI CTAG 3 cut(s) 192, 1284, 2859
MaeII ACGT 3 cut(s) 1102, 1965, 3942
MaeIII GTNAC 8 cut(s) 169, 979, 1367, 1547, 1957, 2510, 2801, 3034
MfeI CAATTG 1 cut(s) 1170
MflI RGATCY 3 cut(s) 2539, 2832, 3443
MhlI GDGCHC 7 cut(s) 539, 1345, 2264, 2662, 2955, 3756, 4022
Mly113I GGCGCC 1 cut(s) 701
MlyI GAGTC 8 cut(s) 260, 385, 1151, 2063, 2752, 2945, 3178, 3643
MmeI TCCRAC 6 cut(s) 651, 717, 1134, 1696, 2333, 3557
MroI TCCGGA 1 cut(s) 1360
MroXI GAANNNNTTC 5 cut(s) 297, 485, 1205, 3306, 3742
MslI CAYNNNNRTG 2 cut(s) 1964, 3498
MspA1I CMGCKG 2 cut(s) 315, 3901
MspCI CTTAAG 1 cut(s) 1346
MspI CCGG 8 cut(s) 804, 1355, 1361, 1463, 2258, 2375, 2960, 3881
MunI CAATTG 1 cut(s) 1170
Mva1269I GAATGC 3 cut(s) 1285, 1845, 1892
MvaI CCWGG 9 cut(s) 868, 1250, 1922, 2034, 2114, 2731, 2926, 3251, 3340
MvnI CGCG 1 cut(s) 3918
NarI GGCGCC 1 cut(s) 701
NciI CCSGG 5 cut(s) 1355, 1356, 1463, 2259, 2961
NcoI CCATGG 1 cut(s) 398
NdeI CATATG 1 cut(s) 3990
NmuCI GTSAC 4 cut(s) 169, 1957, 2510, 2801
NsbI TGCGCA 1 cut(s) 62
NspI RCATGY 4 cut(s) 1415, 1520, 2951, 4088
NspV TTCGAA 2 cut(s) 1708, 3736
OliI CACNNNNGTG 1 cut(s) 1964
PaeI GCATGC 1 cut(s) 1520
PagI TCATGA 3 cut(s) 1836, 2391, 3760
PceI AGGCCT 2 cut(s) 1991, 3613
PciI ACATGT 1 cut(s) 2947
PciSI GCTCTTC 2 cut(s) 1101, 1211
PctI GAATGC 3 cut(s) 1285, 1845, 1892
PdmI GAANNNNTTC 5 cut(s) 297, 485, 1205, 3306, 3742
PfoI TCCNGGA 1 cut(s) 1461
PkrI GCNGC 5 cut(s) 150, 1177, 1633, 2308, 3982
PleI GAGTC 8 cut(s) 259, 385, 1151, 2063, 2751, 2944, 3177, 3643
PluTI GGCGCC 1 cut(s) 704
PpsI GAGTC 8 cut(s) 259, 385, 1151, 2063, 2751, 2944, 3177, 3643
PpuMI RGGWCCY 2 cut(s) 2665, 2717
PscI ACATGT 1 cut(s) 2947
PshAI GACNNNNGTC 3 cut(s) 34, 600, 768
PshBI ATTAAT 1 cut(s) 2472
Psp124BI GAGCTC 1 cut(s) 3756
Psp5II RGGWCCY 2 cut(s) 2665, 2717
Psp6I CCWGG 9 cut(s) 866, 1248, 1920, 2032, 2112, 2729, 2924, 3249, 3338
PspFI CCCAGC 2 cut(s) 2846, 3948
PspGI CCWGG 9 cut(s) 866, 1248, 1920, 2032, 2112, 2729, 2924, 3249, 3338
PspOMI GGGCCC 2 cut(s) 535, 4018
PspPPI RGGWCCY 2 cut(s) 2665, 2717
PstI CTGCAG 2 cut(s) 1180, 3714
PstNI CAGNNNCTG 2 cut(s) 394, 2668
PsuI RGATCY 3 cut(s) 2539, 2832, 3443
PvuII CAGCTG 1 cut(s) 315
RsaI GTAC 6 cut(s) 323, 2140, 2503, 2857, 3409, 3589
RsaNI GTAC 6 cut(s) 322, 2139, 2502, 2856, 3408, 3588
RseI CAYNNNNRTG 2 cut(s) 1964, 3498
SacI GAGCTC 1 cut(s) 3756
SalI GTCGAC 1 cut(s) 1104
SapI GCTCTTC 2 cut(s) 1101, 1211
SatI GCNGC 5 cut(s) 149, 1176, 1632, 2307, 3981
ScaI AGTACT 3 cut(s) 2140, 2857, 3409
SchI GAGTC 8 cut(s) 260, 385, 1151, 2063, 2752, 2945, 3178, 3643
SduI GDGCHC 7 cut(s) 539, 1345, 2264, 2662, 2955, 3756, 4022
SexAI ACCWGGT 2 cut(s) 1248, 3338
SfaNI GCATC 8 cut(s) 562, 787, 1036, 2317, 3502, 3676, 3862, 4066
SfcI CTRYAG 4 cut(s) 1176, 1296, 1993, 3710
SfoI GGCGCC 1 cut(s) 702
SfuI TTCGAA 2 cut(s) 1708, 3736
SinI GGWCC 6 cut(s) 1220, 1358, 2036, 2665, 2717, 3957
SmaI CCCGGG 1 cut(s) 1356
SmiMI CAYNNNNRTG 2 cut(s) 1964, 3498
SmlI CTYRAG 2 cut(s) 613, 1346
SmoI CTYRAG 2 cut(s) 613, 1346
SpeI ACTAGT 1 cut(s) 191
SphI GCATGC 1 cut(s) 1520
SseBI AGGCCT 2 cut(s) 1991, 3613
SsiI CCGC 9 cut(s) 453, 761, 1588, 1631, 1790, 2063, 2402, 3899, 3916
SspDI GGCGCC 1 cut(s) 700
SspI AATATT 3 cut(s) 648, 958, 3091
SspMI CTAG 3 cut(s) 192, 1284, 2859
SstI GAGCTC 1 cut(s) 3756
StuI AGGCCT 2 cut(s) 1991, 3613
StyI CCWWGG 3 cut(s) 398, 3323, 3644
TaiI ACGT 3 cut(s) 1105, 1968, 3945
TaqI TCGA 9 cut(s) 988, 1037, 1105, 1708, 2095, 2297, 2323, 3136, 3736
TatI WGTACW 3 cut(s) 2138, 2855, 3407
TauI GCSGC 1 cut(s) 1634
TscAI CASTG 9 cut(s) 178, 437, 2009, 2531, 3004, 3303, 3360, 3404, 3732
TseFI GTSAC 4 cut(s) 169, 1957, 2510, 2801
TseI GCWGC 4 cut(s) 148, 1175, 2306, 3980
Tsp45I GTSAC 4 cut(s) 169, 1957, 2510, 2801
TspGWI ACGGA 1 cut(s) 2264
TspMI CCCGGG 1 cut(s) 1354
TspRI CASTG 9 cut(s) 178, 437, 2009, 2531, 3004, 3303, 3360, 3404, 3732
Vha464I CTTAAG 1 cut(s) 1346
VpaK11BI GGWCC 6 cut(s) 1220, 1358, 2036, 2665, 2717, 3957
VspI ATTAAT 1 cut(s) 2472
XapI RAATTY 8 cut(s) 835, 1653, 1914, 2343, 2929, 3232, 3284, 3808
XceI RCATGY 4 cut(s) 1415, 1520, 2951, 4088
XmaI CCCGGG 1 cut(s) 1354
XmiI GTMKAC 3 cut(s) 1105, 1865, 1973
XmnI GAANNNNTTC 5 cut(s) 297, 485, 1205, 3306, 3742
XspI CTAG 3 cut(s) 192, 1284, 2859
ZrmI AGTACT 3 cut(s) 2140, 2857, 3409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.