Rh4CG030300

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000147
Physical Location & Seq
Reverse (-)
6034364 .. 6035230
867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG030300.1

Sequence Viewer

Length: 549 bp
ATGGAGACTCGACAGCAAGAATCAGCACTCAAGCTTTGGATGCGTCAACTTGGTTTTGACCATTTCTTTGTAGTTGATCCTACAAATAATGGGGGTGGTGGTCTTGCTCTCTTTTGGGACAACTCAGTCCAAGTCTCTAGTATTAGCGGCACGGTGGTTATAAAGGAAAGGTTGGACCGCGCCCTTGGCAACAATGCTTGGATTGCAAATCAACCGAACACCCAAGTCTTTCACCTTCATTTACTTGGTTCAGATCATCGTCCAATTATGCTGGACACTAAACCCACGGAGCGTTTGACTCCGCCTATCTTCCGCTTCGAACATTTGTGGACTACATACCCATCTAGCATACAGATTGTCCAAGCCAATTGGCAGACTTCTTCTTCCCCATCACAGGCTACCAACTGGGCTTCCAATCTCAATGACTGTCAAGCTTCCCTCTCCTCTTGGGCAAAAGATACTTTTCCAAATTTTGGCAAACTTGCTTCTGACGGCCATAGCAGGATCCAGCTTCTTTTGGACTCTGATCTTCCTGATGCCCATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

182

Amino Acids

20.44

Weight (kDa)

5.92

Isoelectric Point (pI)

23.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000147)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10354 FvH4_1g17131 FvH4_2g11611 FvH4_2g33851 FvH4_3g32112 FvH4_3g35052 FvH4_3g44611 FvH4_4g05921 FvH4_4g06411 FvH4_4g06792 FvH4_4g10365 FvH4_4g21422 FvH4_4g22401 FvH4_4g30261 FvH4_5g08843 FvH4_5g14731 FvH4_5g24781 FvH4_5g26231 FvH4_5g31282 FvH4_6g23371 FvH4_6g38511 FvH4_7g01171 FvH4_7g03341 FvH4_7g06171 FvH4_7g32472
prunus_persica Prupe.2G036900_v2.0.a1
pyrus_communis pycom02g19900 pycom03g01520 pycom05g10510 pycom16g23820
rosa_chinensis RchiOBHm_Chr1g0316031 RchiOBHm_Chr1g0342451 RchiOBHm_Chr1g0344541 RchiOBHm_Chr1g0364721 RchiOBHm_Chr2g0133541 RchiOBHm_Chr2g0134511 RchiOBHm_Chr2g0145891 RchiOBHm_Chr2g0163241 RchiOBHm_Chr5g0027201 RchiOBHm_Chr5g0027881 RchiOBHm_Chr5g0047471 RchiOBHm_Chr7g0210901
rosa_laevigata RLG00000011116 RLG00000017964 RLG00000018128 RLG00000018387 RLG00000018829 RLG00000019353 RLG00000028973 RLG00000035870 RLG00000036629
rosa_multiflora Rmu_co8126340.1_g000001 Rmu_co8216750.1_g000001 Rmu_co8222072.1_g000001 Rmu_co8332453.1_g000001 Rmu_co8349913.1_g000001 Rmu_co8405337.1_g000001 Rmu_sc0000041.1_g000012 Rmu_sc0000166.1_g000029 Rmu_sc0000251.1_g000019 Rmu_sc0000271.1_g000028 Rmu_sc0000509.1_g000036 Rmu_sc0000543.1_g000048 Rmu_sc0000808.1_g000003 Rmu_sc0000847.1_g000040 Rmu_sc0000972.1_g000020 Rmu_sc0001260.1_g000001 Rmu_sc0001464.1_g000026 Rmu_sc0001616.1_g000003 Rmu_sc0001669.1_g000001 Rmu_sc0002005.1_g000001 Rmu_sc0002014.1_g000010 Rmu_sc0002082.1_g000033 Rmu_sc0002302.1_g000024 Rmu_sc0002531.1_g000045 Rmu_sc0003276.1_g000002 Rmu_sc0003737.1_g000007 Rmu_sc0003832.1_g000022 Rmu_sc0004080.1_g000011 Rmu_sc0004487.1_g000009 Rmu_sc0004988.1_g000018 Rmu_sc0004988.1_g000019 Rmu_sc0005254.1_g000002 Rmu_sc0005733.1_g000004 Rmu_sc0006413.1_g000006 Rmu_sc0007121.1_g000013 Rmu_sc0007869.1_g000006 Rmu_sc0008035.1_g000015 Rmu_sc0008279.1_g000017 Rmu_sc0008351.1_g000014 Rmu_sc0008957.1_g000007 Rmu_sc0009012.1_g000007 Rmu_sc0010556.1_g000009 Rmu_sc0012889.1_g000003 Rmu_sc0019808.1_g000004 Rmu_sc0028113.1_g000001 Rmu_sc0031202.1_g000001 Rmu_sc0034436.1_g000001 Rmu_sc0034445.1_g000001 Rmu_sc0040044.1_g000001 Rmu_ssc0000062.1_g000032 Rmu_ssc0000372.1_g000018
rosa_roxburghii Rroxscaffold_1G00001520 Rroxscaffold_1G00039990 Rroxscaffold_1G00046370 Rroxscaffold_2G00110140 Rroxscaffold_2G00131420 Rroxscaffold_3G00222390 Rroxscaffold_5G00336040 Rroxscaffold_5G00338000 Rroxscaffold_5G00344030 Rroxscaffold_5G00363460 Rroxscaffold_6G00402000 Rroxscaffold_7G00194940
rosa_rugosa Rorug01G0106500 Rorug01G0111300 Rorug01G0247000 Rorug02G0318100.1 Rorug02G0318200 Rorug02G0459500 Rorug03G0108100 Rorug03G0200500 Rorug03G0232500 Rorug03G0240600 Rorug04G0105300 Rorug04G0256600 Rorug06G0079300 Rorug06G0082700 Rorug06G0095600 Rorug06G0179000 Rorug07G0113500 Rorug07G0271100
rosa_samantha Rh1AG087200 Rh1CG085300 Rh1DG013200 Rh1DG091700 Rh1DG091800 Rh1DG338300 Rh2AG375400 Rh2AG407000 Rh2AG513500 Rh2BG277700 Rh2BG371200 Rh2BG376100 Rh2BG580500 Rh2CG354300 Rh2DG200100 Rh2DG388000 Rh2DG393500 Rh2DG426400 Rh3CG304800 Rh4AG044600 Rh4AG220500 Rh4BG379300 Rh4CG007100 Rh4CG030300 Rh4CG038000 Rh4CG114900 Rh4CG393700 Rh5AG124900 Rh5AG342300 Rh5BG414400 Rh5CG263800 Rh5CG439000 Rh5DG231100 Rh5DG566900 Rh6AG138600 Rh6AG200000 Rh6AG253100 Rh6AG390900 Rh6AG441600 Rh6BG194700 Rh6BG225800 Rh6BG256100 Rh6CG231700 Rh6CG231900 Rh6CG312200 Rh6CG453700 Rh6DG219400 Rh7AG357700 Rh7AG442000 Rh7CG425700 Rh7CG474600 Rh7CG494100 Rh7DG253900 Rh7DG294700 Rh7DG401600 Rh7DG461300
rosa_wichuraiana Rw1G001190 Rw2G047020 Rw4G003490 Rw5G039690 Rw6G010450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 161
AasI GACNNNNNNGTC 1 cut(s) 125
AccB7I CCANNNNNTGG 1 cut(s) 473
AccII CGCG 1 cut(s) 180
AciI CCGC 4 cut(s) 147, 178, 302, 313
AclWI GGATC 3 cut(s) 71, 499, 512
AcoI YGGCCR 1 cut(s) 493
AcsI RAATTY 1 cut(s) 469
AfiI CCNNNNNNNGG 2 cut(s) 394, 473
AluBI AGCT 3 cut(s) 34, 434, 511
AluI AGCT 3 cut(s) 34, 434, 511
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 3 cut(s) 71, 499, 512
AoxI GGCC 1 cut(s) 493
ApoI RAATTY 1 cut(s) 469
ArsI GACNNNNNNTTYG 2 cut(s) 50, 82
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 1 cut(s) 175
AsuHPI GGTGA 1 cut(s) 224
AsuII TTCGAA 1 cut(s) 318
AvaII GGWCC 1 cut(s) 175
BamHI GGATCC 1 cut(s) 504
BccI CCATC 2 cut(s) 349, 397
BceAI ACGGC 1 cut(s) 508
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 2 cut(s) 138, 345
BisI GCNGC 1 cut(s) 148
BlsI GCNGC 1 cut(s) 149
Bme18I GGWCC 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 175
BmiI GGNNCC 1 cut(s) 506
BmrI ACTGGG 1 cut(s) 415
BmsI GCATC 2 cut(s) 30, 526
BmuI ACTGGG 1 cut(s) 415
Bpu14I TTCGAA 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 14
BsaBI GATNNNNATC 1 cut(s) 540
BsaJI CCNNGG 2 cut(s) 184, 285
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 473
Bse1I ACTGG 1 cut(s) 410
Bse8I GATNNNNATC 1 cut(s) 540
BseDI CCNNGG 2 cut(s) 184, 285
BseGI GGATG 1 cut(s) 45
BseJI GATNNNNATC 1 cut(s) 540
BseLI CCNNNNNNNGG 2 cut(s) 394, 473
BseMII CTCAG 1 cut(s) 138
BseNI ACTGG 1 cut(s) 410
BseRI GAGGAG 1 cut(s) 433
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 1 cut(s) 495
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 394, 473
BsmAI GTCTC 1 cut(s) 139
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 1 cut(s) 495
Bsp119I TTCGAA 1 cut(s) 318
Bsp143I GATC 4 cut(s) 76, 253, 504, 526
BspACI CCGC 4 cut(s) 147, 178, 302, 313
BspANI GGCC 1 cut(s) 495
BspCNI CTCAG 1 cut(s) 137
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 506
BspPI GGATC 3 cut(s) 71, 499, 512
BspT104I TTCGAA 1 cut(s) 318
BsrI ACTGG 1 cut(s) 410
BssECI CCNNGG 2 cut(s) 184, 285
BssMI GATC 4 cut(s) 76, 253, 504, 526
BssT1I CCWWGG 1 cut(s) 184
Bst4CI ACNGT 2 cut(s) 154, 428
BstBI TTCGAA 1 cut(s) 318
BstDEI CTNAG 1 cut(s) 124
BstDSI CCRYGG 1 cut(s) 285
BstF5I GGATG 1 cut(s) 45
BstFNI CGCG 1 cut(s) 180
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 4 cut(s) 79, 256, 507, 529
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 4 cut(s) 76, 253, 504, 526
BstMWI GCNNNNNNNGC 3 cut(s) 40, 186, 203
BstUI CGCG 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 504
BstYI RGATCY 1 cut(s) 504
BsuRI GGCC 1 cut(s) 495
BtgI CCRYGG 1 cut(s) 285
BtsCI GGATG 1 cut(s) 45
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 175
CseI GACGC 1 cut(s) 32
CviJI RGCY 7 cut(s) 34, 365, 398, 410, 434, 495, 511
CviKI_1 RGCY 7 cut(s) 34, 365, 398, 410, 434, 495, 511
DdeI CTNAG 1 cut(s) 124
DpnI GATC 4 cut(s) 78, 255, 506, 528
DpnII GATC 4 cut(s) 76, 253, 504, 526
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 1 cut(s) 493
EciI GGCGGA 1 cut(s) 291
Eco130I CCWWGG 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 184
FaiI YATR 5 cut(s) 161, 269, 337, 350, 498
FaqI GGGAC 1 cut(s) 131
Fnu4HI GCNGC 1 cut(s) 148
FokI GGATG 1 cut(s) 52
Fsp4HI GCNGC 1 cut(s) 148
FspBI CTAG 2 cut(s) 138, 345
GlaI GCGC 1 cut(s) 181
GluI GCNGC 1 cut(s) 148
HaeIII GGCC 1 cut(s) 495
HgaI GACGC 1 cut(s) 32
HhaI GCGC 1 cut(s) 182
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 1 cut(s) 47
HindII GTYRAC 1 cut(s) 47
HindIII AAGCTT 2 cut(s) 32, 432
HinfI GANTC 4 cut(s) 7, 20, 298, 521
HphI GGTGA 1 cut(s) 224
Hpy166II GTNNAC 2 cut(s) 47, 330
Hpy188I TCNGA 3 cut(s) 253, 490, 526
Hpy188III TCNNGA 1 cut(s) 533
Hpy8I GTNNAC 2 cut(s) 47, 330
HpyAV CCTTC 1 cut(s) 245
HpyCH4III ACNGT 2 cut(s) 154, 428
HpyCH4V TGCA 1 cut(s) 206
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 186, 203
HpyF3I CTNAG 1 cut(s) 124
HspAI GCGC 1 cut(s) 180
Kzo9I GATC 4 cut(s) 76, 253, 504, 526
LmnI GCTCC 1 cut(s) 289
LpnPI CCDG 5 cut(s) 257, 380, 391, 487, 521
LweI GCATC 2 cut(s) 30, 526
MaeI CTAG 2 cut(s) 138, 345
MalI GATC 4 cut(s) 78, 255, 506, 528
MboI GATC 4 cut(s) 76, 253, 504, 526
MboII GAAGA 4 cut(s) 301, 372, 375, 521
MfeI CAATTG 1 cut(s) 367
MflI RGATCY 1 cut(s) 504
MluCI AATT 3 cut(s) 264, 367, 469
MlyI GAGTC 2 cut(s) 292, 515
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 2 cut(s) 449, 454
MunI CAATTG 1 cut(s) 367
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 3 cut(s) 40, 186, 203
NdeII GATC 4 cut(s) 76, 253, 504, 526
NlaIV GGNNCC 1 cut(s) 506
NspV TTCGAA 1 cut(s) 318
PfeI GAWTC 1 cut(s) 20
PflMI CCANNNNNTGG 1 cut(s) 473
PkrI GCNGC 1 cut(s) 149
PleI GAGTC 2 cut(s) 292, 515
PpsI GAGTC 2 cut(s) 292, 515
PsiI TTATAA 1 cut(s) 161
PspN4I GGNNCC 1 cut(s) 506
PspPI GGNCC 1 cut(s) 175
PsuI RGATCY 1 cut(s) 504
SatI GCNGC 1 cut(s) 148
Sau3AI GATC 4 cut(s) 76, 253, 504, 526
Sau96I GGNCC 1 cut(s) 175
SchI GAGTC 2 cut(s) 292, 515
SetI ASST 5 cut(s) 36, 173, 237, 436, 513
SfaNI GCATC 2 cut(s) 30, 526
SfuI TTCGAA 1 cut(s) 318
SinI GGWCC 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 3 cut(s) 264, 367, 469
SsiI CCGC 4 cut(s) 147, 178, 302, 313
SspMI CTAG 2 cut(s) 138, 345
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 2 cut(s) 154, 428
TaqI TCGA 2 cut(s) 10, 318
TasI AATT 3 cut(s) 264, 367, 469
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 1 cut(s) 20
TspDTI ATGAA 1 cut(s) 227
TspGWI ACGGA 1 cut(s) 302
Van91I CCANNNNNTGG 1 cut(s) 473
VpaK11BI GGWCC 1 cut(s) 175
XapI RAATTY 1 cut(s) 469
XspI CTAG 2 cut(s) 138, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.