FvH4_3g11503

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000110
Physical Location & Seq
Forward (+)
6785509 .. 6791053
5545 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11503.t2

Sequence Viewer

Length: 2748 bp
ATGGCCTCGTCTGTAACGGACCTCTTAATTGGAAAACTTGTGACGATTCTGGAGAACGAAGGAACAGCTATAGCCGGTGTCCGTGATGAAGTATATAAGATTAAGGAGGAGTTTCTAAGCATGAAAGCTTTCCTAAAGGATGCGGAGGCCAACAAACCAAAAACTGAAGGAGAGCAGTTGTGGGTTGGTAGAATCAGAGACTTAGCCCATGATGTTGAAGATATCATCGATGAATTCTTGTATCACATGTATGAGCAGAAGCAAAGTGGGGGTCGACTTTCAAGAGGCCTGCACAAAACCATTCGAGCTCCAAGTAATCTTTGGTTCAGGCATAAAATTGCAAAGAAGTTGCAGAAAATTACTCAAATGATTAAAGATATTCCAGAGAGGAATCAAAGGTACGGTGTTGGCCTTACAGGAGGAGCGACTACATCTGAAGATAGTCAGAAACCGGGGCTGAACCAAGCGGAGTATTCGTTCTCCATTACGGAAGAGGAACTGGTTGGGATTGAAGGCAAGAAGCAAATACTAATGGGATGGCTGATGGATGAAGAGCAACACCAAACTGTAATATCTGTGGTTGGCATGGGGGGTTCTGGGAAGACAACTCTAGCTGCCAAGACCTTCAACAATGAAAGGGTACAGAGACATTTCCATTGTTATGCATGGATTACCGTTTCTGAAACTTATGTTACTGAAGACTTGTTTCGAAGTCTAATCAAGGAACTCCACAAATCAAAGAAGGAAGATATTCCAGACTTGAATGCCATGAACCGCACAGATTTACGAGATGTACTTGTTAACTACTTGGAGTCCAAACGGTATTTGATTGTTCTAGATGATGTGTGGAATATCGAAGTCTGGAAAGAAGTAAGGATAGCACTTCATGATAAAGGACTTGGAAGTCGAATCATACTGACAACCCGAAAAGAAGACATAGCATCTTATTTCGGAGTAAAAGGCCATGTTCACCATATTCAACCCCTGAAAAGGTATGAGGCTTGGGAGCTCTTCAGCCAGAAAGCATTCTCATCTAATGATAACAAAGCTTGTCCATCCGAGCTCAAGTCATTAGCTTGGGAACTGGTGGAGAAGTGTGAAGGCCTTCCTCTGGCTATCGTGGCTTTAGGCGGTGTTATGTCTTCAAAGAAGTCACCAGATGAATGGCACAGAGTCTTGAAAAGTATAAATTGGCATCTAAATAACAACTCATTGCTTGGCCCTGTCAAAACCTTGTTGTTGCTTAGTTACAATGATTTGCCATCCCAGTTGAAGCATTGTTTCCTTTATTGTTCCCTCTTACCAGAAGATTATTTGATTCAACGAAAAAGGTTGATTAAGCTGTGGATAGCTGAAGGATTTGTTGAACATGCAAACGGGGTCACGCCAGAAGAGGTTGCGGGTGGCTATCTTCTGGAACTCTGCTTCCGTAGCATGCTACAAGTTGCTATGAGAAATGGAGAAGGAAGGCCGAAAAAAGTTAAGATGCATGATCTTCTACGAGAGCTTGCTCTGTCGATATCCGAAAAAGAAAAATTTGGACTTGTATATGATGGAAGACAAGTAATGGAAGAGATCACAACCCGTCGCTTGTCAATTCACACAACGAGTGACGGAGGAGGAATTAAATCAGGGCCGGGTATGTCAAACATTCGTTCTCTTCTTGTCTTTGCAACTTACATGTCCTCAAATGAACTGCTTTCTCGATTCAAATTGCTGAGAACTCTAGACTTGGAGGATGTCCAAATTGATAAACTCCCAGATGCAGTTGTTTACTTGTTCAACTTGAGATATTTAAGCCTGCGGGGCTGCTCTCTAATCAAAGAACTTCCAGAGTCCATAGGGCGTCTCCGCAACCTTCAATTTCTGAACATCAGGAATACCAAGATAGAGTCACTTCCACAAGGAATTGCAAAATTGCTCAACCTGCGCCATCTCATTATGTATCGCCAGACCGGAAATTTTCAGGTCTTTGAATACGAGGTTGGGACAAAAGCGCCATCAAATATTTGCCAGTTGCAGAAATTGCAATGTTTAATAACTATTGAATCAGACGGGGACACTATCAGATTAATTGGGAACCTGACTCAACTTACGACCATCGGCATATCAAATTTGAAAGAAACAGATGAGATTGACCTTTGGGTCTCAATTAAGAAGCTGACTCTGCTTCAAGCTCTAGCACTCCAGGCAAGTAATGAAGAGGAATTTCTTCCTGTGAAGGCAGAATGTCCACCTCTTCCGCACCTTCAAAAGCTTTATTTGACAGGTAGACTGCAAAAAGTAGTACCTTGGTTTTCTTCCCTGCATAGCCTCACATTTCTGCAGCTGCATTGGTCTAGACTTGAAGACGATGTACTACCTCACATTGAAGCACTTCCCAATCTGACGAAGCTTGATCTTAGTAATGCATATGTTGGGGAAGAGTTGTGTTTCCGCAGAGGTTTTGTAAAGCTGGTGACATTGGTGTTGAGTGATTTTGGCTCGTTGAAAAAGATAGCTATGGAAAAAGGGGCCATGCCTAATCTCCGGGACTTACATGTTGGCAGCTGCATGGAATTGAAGGCAGTACCACTGGGCCTTGAAAACCTTTCTAATCTGCAACTCTTGAACCTGAGGACTGTTCCAACAGAAATTATAAACTCCATTCGGAAAGGAGGTGCGGATCGCAAAAATGTACAACACATCCCTAAGATCAGACATGTTTTCCAACAGTCATCCGAGTGGTTCTACGAAAACTTGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

916

Amino Acids

104.16

Weight (kDa)

8.34

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 93 5.5e-22 Rx N-terminal domain
NB-ARC PF00931 173 - 347 4.2e-48 NB-ARC domain
WHD_DRP PF23559 434 - 504 1.4e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 551 - 872 1.4e-43 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2639
AasI GACNNNNNNGTC 2 cut(s) 903, 2144
Acc36I ACCTGC 1 cut(s) 1935
AccI GTMKAC 2 cut(s) 274, 2272
AclWI GGATC 1 cut(s) 2673
AcsI RAATTY 5 cut(s) 233, 1535, 1960, 2113, 2207
AcuI CTGAAG 5 cut(s) 186, 456, 717, 997, 1374
AcyI GRCGYC 1 cut(s) 1846
AfaI GTAC 7 cut(s) 401, 642, 795, 2289, 2358, 2571, 2679
AfiI CCNNNNNNNGG 2 cut(s) 990, 1111
AflIII ACRYGT 4 cut(s) 246, 1680, 2539, 2701
AjnI CCWGG 1 cut(s) 2187
AjuI GAANNNNNNNTTGG 2 cut(s) 1968, 2000
AloI GAACNNNNNNTCC 4 cut(s) 461, 493, 1410, 1442
Alw21I GWGCWC 3 cut(s) 310, 1011, 1065
Alw26I GTCTC 4 cut(s) 192, 640, 1853, 2152
AlwI GGATC 1 cut(s) 2673
ApeKI GCWGC 6 cut(s) 614, 1809, 2326, 2329, 2547, 2550
ApoI RAATTY 5 cut(s) 233, 1535, 1960, 2113, 2207
ArsI GACNNNNNNTTYG 2 cut(s) 256, 288
AseI ATTAAT 1 cut(s) 2072
Asp700I GAANNNNTTC 6 cut(s) 128, 750, 1025, 1104, 2211, 2376
AspLEI GCGC 2 cut(s) 1932, 1999
AspS9I GGNCC 5 cut(s) 19, 1220, 1634, 2514, 2578
AsuC2I CCSGG 3 cut(s) 453, 1638, 2531
AsuHPI GGTGA 3 cut(s) 962, 1146, 2470
AsuII TTCGAA 1 cut(s) 709
AvaII GGWCC 1 cut(s) 19
AxyI CCTNAGG 1 cut(s) 2615
BanII GRGCYC 3 cut(s) 310, 1011, 1065
BarI GAAGNNNNNNTAC 2 cut(s) 2340, 2372
BbsI GAAGAC 6 cut(s) 608, 705, 939, 1134, 1564, 2355
Bbv12I GWGCWC 3 cut(s) 310, 1011, 1065
BbvI GCAGC 6 cut(s) 601, 1796, 2316, 2338, 2537, 2559
BccI CCATC 8 cut(s) 531, 538, 1063, 1270, 1547, 1941, 2008, 2108
BciT130I CCWGG 1 cut(s) 2189
BcnI CCSGG 3 cut(s) 453, 1638, 2531
BcoDI GTCTC 4 cut(s) 192, 640, 1853, 2152
BfaI CTAG 6 cut(s) 611, 836, 1727, 2180, 2340, 2746
BfmI CTRYAG 2 cut(s) 69, 2324
BfoI RGCGCY 1 cut(s) 2000
BfuAI ACCTGC 1 cut(s) 1935
BglI GCCNNNNNGGC 1 cut(s) 1806
BisI GCNGC 6 cut(s) 615, 1810, 2327, 2330, 2548, 2551
BlsI GCNGC 6 cut(s) 616, 1811, 2328, 2331, 2549, 2552
Bme1390I CCNGG 4 cut(s) 453, 1638, 2189, 2531
Bme18I GGWCC 1 cut(s) 19
BmgT120I GGNCC 5 cut(s) 19, 1220, 1634, 2514, 2578
BmiI GGNNCC 2 cut(s) 2081, 2515
BmrFI CCNGG 4 cut(s) 453, 1638, 2189, 2531
BmrI ACTGGG 2 cut(s) 1261, 2585
BmsI GCATC 5 cut(s) 130, 950, 1204, 1476, 1753
BmuI ACTGGG 2 cut(s) 1261, 2585
BpiI GAAGAC 6 cut(s) 608, 705, 939, 1134, 1564, 2355
BpmI CTGGAG 2 cut(s) 71, 2171
Bpu14I TTCGAA 1 cut(s) 709
BpuEI CTTGAG 2 cut(s) 1049, 1807
BpuMI CCSGG 3 cut(s) 453, 1638, 2531
Bsa29I ATCGAT 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 1846
BsaI GGTCTC 1 cut(s) 2152
BsaJI CCNNGG 2 cut(s) 452, 2291
BsaWI WCCGGW 1 cut(s) 1955
BsaXI ACNNNNNCTCC 2 cut(s) 461, 491
Bsc4I CCNNNNNNNGG 2 cut(s) 990, 1111
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 5 cut(s) 504, 1089, 1267, 2014, 2580
Bse21I CCTNAGG 1 cut(s) 2615
Bse3DI GCAATG 2 cut(s) 1211, 2036
BseBI CCWGG 1 cut(s) 2189
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 2 cut(s) 452, 2291
BseGI GGATG 8 cut(s) 145, 542, 553, 1055, 1262, 1744, 2685, 2717
BseLI CCNNNNNNNGG 2 cut(s) 990, 1111
BseMI GCAATG 2 cut(s) 1211, 2036
BseMII CTCAG 2 cut(s) 1709, 2606
BseNI ACTGG 5 cut(s) 504, 1089, 1267, 2014, 2580
BseRI GAGGAG 3 cut(s) 122, 435, 1632
BseXI GCAGC 6 cut(s) 601, 1796, 2316, 2338, 2537, 2559
BsgI GTGCAG 1 cut(s) 275
BshVI ATCGAT 1 cut(s) 228
BsiHKAI GWGCWC 3 cut(s) 310, 1011, 1065
BsiSI CCGG 5 cut(s) 75, 452, 1637, 1956, 2530
BslFI GGGAC 3 cut(s) 2002, 2072, 2546
BslI CCNNNNNNNGG 2 cut(s) 990, 1111
BsmAI GTCTC 4 cut(s) 192, 640, 1853, 2152
BsmBI CGTCTC 1 cut(s) 1853
BsmFI GGGAC 3 cut(s) 2002, 2072, 2546
BsmI GAATGC 2 cut(s) 769, 1025
Bso31I GGTCTC 1 cut(s) 2152
Bsp119I TTCGAA 1 cut(s) 709
Bsp1286I GDGCHC 3 cut(s) 310, 1011, 1065
Bsp1407I TGTACA 1 cut(s) 2677
Bsp143I GATC 5 cut(s) 1492, 1575, 2398, 2665, 2694
BspCNI CTCAG 2 cut(s) 1710, 2607
BspDI ATCGAT 1 cut(s) 228
BspHI TCATGA 1 cut(s) 886
BspLI GGNNCC 2 cut(s) 2081, 2515
BspMAI CTGCAG 1 cut(s) 2328
BspMI ACCTGC 1 cut(s) 1935
BspPI GGATC 1 cut(s) 2673
BspQI GCTCTTC 2 cut(s) 546, 1016
BspT104I TTCGAA 1 cut(s) 709
BspTNI GGTCTC 1 cut(s) 2152
BsrDI GCAATG 2 cut(s) 1211, 2036
BsrFI RCCGGY 1 cut(s) 74
BsrGI TGTACA 1 cut(s) 2677
BsrI ACTGG 5 cut(s) 504, 1089, 1267, 2014, 2580
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 2 cut(s) 452, 2291
BssMI GATC 5 cut(s) 1492, 1575, 2398, 2665, 2694
BssNI GRCGYC 1 cut(s) 1846
BssT1I CCWWGG 1 cut(s) 2291
Bst2UI CCWGG 1 cut(s) 2189
Bst4CI ACNGT 6 cut(s) 404, 568, 676, 822, 2623, 2715
Bst6I CTCTTC 9 cut(s) 486, 546, 1016, 1386, 1566, 1665, 2196, 2244, 2418
BstACI GRCGYC 1 cut(s) 1846
BstAPI GCANNNNNTGC 1 cut(s) 2026
BstAUI TGTACA 1 cut(s) 2677
BstBI TTCGAA 1 cut(s) 709
BstC8I GCNNGC 4 cut(s) 290, 1436, 1509, 1802
BstDEI CTNAG 7 cut(s) 116, 202, 1244, 1718, 2402, 2615, 2691
BstF5I GGATG 8 cut(s) 145, 542, 553, 1055, 1262, 1744, 2685, 2717
BstH2I RGCGCY 1 cut(s) 2000
BstHHI GCGC 2 cut(s) 1932, 1999
BstKTI GATC 5 cut(s) 1495, 1578, 2401, 2668, 2697
BstMAI GTCTC 4 cut(s) 192, 640, 1853, 2152
BstMBI GATC 5 cut(s) 1492, 1575, 2398, 2665, 2694
BstMWI GCNNNNNNNGC 7 cut(s) 1121, 1431, 1806, 1927, 2026, 2167, 2189
BstNI CCWGG 1 cut(s) 2189
BstNSI RCATGY 6 cut(s) 250, 1373, 1438, 1684, 2543, 2705
BstSCI CCNGG 4 cut(s) 451, 1636, 2187, 2529
BstSFI CTRYAG 2 cut(s) 69, 2324
BstV1I GCAGC 6 cut(s) 601, 1796, 2316, 2338, 2537, 2559
BstV2I GAAGAC 6 cut(s) 608, 705, 939, 1134, 1564, 2355
BstXI CCANNNNNNTGG 1 cut(s) 1164
Bsu15I ATCGAT 1 cut(s) 228
Bsu36I CCTNAGG 1 cut(s) 2615
BsuTUI ATCGAT 1 cut(s) 228
BtsCI GGATG 8 cut(s) 145, 542, 553, 1055, 1262, 1744, 2685, 2717
BtsIMutI CAGTG 1 cut(s) 2573
BveI ACCTGC 1 cut(s) 1935
Cac8I GCNNGC 4 cut(s) 290, 1436, 1509, 1802
CciI TCATGA 1 cut(s) 886
CfoI GCGC 2 cut(s) 1932, 1999
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 5 cut(s) 19, 1220, 1634, 2514, 2578
ClaI ATCGAT 1 cut(s) 228
CseI GACGC 1 cut(s) 1835
Csp6I GTAC 7 cut(s) 400, 641, 794, 2288, 2357, 2570, 2678
CviQI GTAC 7 cut(s) 400, 641, 794, 2288, 2357, 2570, 2678
DdeI CTNAG 7 cut(s) 116, 202, 1244, 1718, 2402, 2615, 2691
DpnI GATC 5 cut(s) 1494, 1577, 2400, 2667, 2696
DpnII GATC 5 cut(s) 1492, 1575, 2398, 2665, 2694
DrdI GACNNNNNNGTC 2 cut(s) 903, 2144
DseDI GACNNNNNNGTC 2 cut(s) 903, 2144
Eam1104I CTCTTC 9 cut(s) 486, 546, 1016, 1386, 1566, 1665, 2196, 2244, 2418
EarI CTCTTC 9 cut(s) 486, 546, 1016, 1386, 1566, 1665, 2196, 2244, 2418
Ecl136II GAGCTC 3 cut(s) 308, 1009, 1063
Eco130I CCWWGG 1 cut(s) 2291
Eco147I AGGCCT 2 cut(s) 288, 1104
Eco24I GRGCYC 3 cut(s) 310, 1011, 1065
Eco31I GGTCTC 1 cut(s) 2152
Eco32I GATATC 2 cut(s) 223, 1521
Eco47I GGWCC 1 cut(s) 19
Eco53kI GAGCTC 3 cut(s) 308, 1009, 1063
Eco57I CTGAAG 5 cut(s) 186, 456, 717, 997, 1374
Eco81I CCTNAGG 1 cut(s) 2615
EcoICRI GAGCTC 3 cut(s) 308, 1009, 1063
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 2187
EcoRV GATATC 2 cut(s) 223, 1521
EcoT14I CCWWGG 1 cut(s) 2291
EcoT22I ATGCAT 3 cut(s) 667, 1491, 2413
EcoT38I GRGCYC 3 cut(s) 310, 1011, 1065
ErhI CCWWGG 1 cut(s) 2291
Esp3I CGTCTC 1 cut(s) 1853
FaqI GGGAC 3 cut(s) 2002, 2072, 2546
FauI CCCGC 2 cut(s) 1393, 1797
FauNDI CATATG 1 cut(s) 2413
FblI GTMKAC 2 cut(s) 274, 2272
Fnu4HI GCNGC 6 cut(s) 615, 1810, 2327, 2330, 2548, 2551
FokI GGATG 8 cut(s) 152, 549, 560, 1042, 1249, 1751, 2672, 2704
FriOI GRGCYC 3 cut(s) 310, 1011, 1065
Fsp4HI GCNGC 6 cut(s) 615, 1810, 2327, 2330, 2548, 2551
FspBI CTAG 6 cut(s) 611, 836, 1727, 2180, 2340, 2746
GlaI GCGC 2 cut(s) 1931, 1998
GluI GCNGC 6 cut(s) 615, 1810, 2327, 2330, 2548, 2551
GsuI CTGGAG 2 cut(s) 71, 2171
HaeII RGCGCY 1 cut(s) 2000
HapII CCGG 5 cut(s) 75, 452, 1637, 1956, 2530
HgaI GACGC 1 cut(s) 1835
HhaI GCGC 2 cut(s) 1932, 1999
Hin1I GRCGYC 1 cut(s) 1846
Hin6I GCGC 2 cut(s) 1930, 1997
HinP1I GCGC 2 cut(s) 1930, 1997
HincII GTYRAC 2 cut(s) 275, 802
HindII GTYRAC 2 cut(s) 275, 802
HindIII AAGCTT 4 cut(s) 126, 1047, 2255, 2393
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 5 cut(s) 75, 452, 1637, 1956, 2530
HphI GGTGA 3 cut(s) 962, 1146, 2470
Hpy166II GTNNAC 6 cut(s) 275, 802, 970, 1774, 2234, 2273
Hpy8I GTNNAC 6 cut(s) 275, 802, 970, 1774, 2234, 2273
Hpy99I CGWCG 1 cut(s) 1590
HpyCH4III ACNGT 6 cut(s) 404, 568, 676, 822, 2623, 2715
HpyF10VI GCNNNNNNNGC 7 cut(s) 1121, 1431, 1806, 1927, 2026, 2167, 2189
HpyF3I CTNAG 7 cut(s) 116, 202, 1244, 1718, 2402, 2615, 2691
Hsp92I GRCGYC 1 cut(s) 1846
HspAI GCGC 2 cut(s) 1930, 1997
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 5 cut(s) 1492, 1575, 2398, 2665, 2694
LguI GCTCTTC 2 cut(s) 546, 1016
LmnI GCTCC 3 cut(s) 313, 422, 1006
Lsp1109I GCAGC 6 cut(s) 601, 1796, 2316, 2338, 2537, 2559
LweI GCATC 5 cut(s) 130, 950, 1204, 1476, 1753
MaeI CTAG 6 cut(s) 611, 836, 1727, 2180, 2340, 2746
MaeIII GTNAC 9 cut(s) 13, 40, 691, 1152, 1247, 1381, 1610, 1893, 2458
MalI GATC 5 cut(s) 1494, 1577, 2400, 2667, 2696
MboI GATC 5 cut(s) 1492, 1575, 2398, 2665, 2694
MhlI GDGCHC 3 cut(s) 310, 1011, 1065
MlyI GAGTC 6 cut(s) 821, 1182, 1844, 1901, 2080, 2158
MmeI TCCRAC 2 cut(s) 2651, 2734
Mph1103I ATGCAT 3 cut(s) 667, 1491, 2413
MroXI GAANNNNTTC 6 cut(s) 128, 750, 1025, 1104, 2211, 2376
MslI CAYNNNNRTG 3 cut(s) 249, 660, 2722
MspA1I CMGCKG 2 cut(s) 2329, 2550
MspI CCGG 5 cut(s) 75, 452, 1637, 1956, 2530
MspR9I CCNGG 4 cut(s) 453, 1638, 2189, 2531
Mva1269I GAATGC 2 cut(s) 769, 1025
MvaI CCWGG 1 cut(s) 2189
MwoI GCNNNNNNNGC 7 cut(s) 1121, 1431, 1806, 1927, 2026, 2167, 2189
NciI CCSGG 3 cut(s) 453, 1638, 2531
NdeI CATATG 1 cut(s) 2413
NdeII GATC 5 cut(s) 1492, 1575, 2398, 2665, 2694
NlaIV GGNNCC 2 cut(s) 2081, 2515
NmuCI GTSAC 6 cut(s) 40, 1152, 1381, 1610, 1893, 2458
NsiI ATGCAT 3 cut(s) 667, 1491, 2413
NspI RCATGY 6 cut(s) 250, 1373, 1438, 1684, 2543, 2705
NspV TTCGAA 1 cut(s) 709
PaeI GCATGC 1 cut(s) 1438
PagI TCATGA 1 cut(s) 886
PceI AGGCCT 2 cut(s) 288, 1104
PciI ACATGT 4 cut(s) 246, 1680, 2539, 2701
PciSI GCTCTTC 2 cut(s) 546, 1016
PctI GAATGC 2 cut(s) 769, 1025
PdmI GAANNNNTTC 6 cut(s) 128, 750, 1025, 1104, 2211, 2376
PfeI GAWTC 7 cut(s) 46, 192, 391, 909, 1318, 1707, 2048
PfoI TCCNGGA 1 cut(s) 2529
PkrI GCNGC 6 cut(s) 616, 1811, 2328, 2331, 2549, 2552
PleI GAGTC 6 cut(s) 820, 1181, 1843, 1900, 2080, 2158
PpsI GAGTC 6 cut(s) 820, 1181, 1843, 1900, 2080, 2158
PscI ACATGT 4 cut(s) 246, 1680, 2539, 2701
PshBI ATTAAT 1 cut(s) 2072
PsiI TTATAA 1 cut(s) 2639
Psp124BI GAGCTC 3 cut(s) 310, 1011, 1065
Psp6I CCWGG 1 cut(s) 2187
PspGI CCWGG 1 cut(s) 2187
PspN4I GGNNCC 2 cut(s) 2081, 2515
PspPI GGNCC 5 cut(s) 19, 1220, 1634, 2514, 2578
PstI CTGCAG 1 cut(s) 2328
PvuII CAGCTG 2 cut(s) 2329, 2550
RsaI GTAC 7 cut(s) 401, 642, 795, 2289, 2358, 2571, 2679
RsaNI GTAC 7 cut(s) 400, 641, 794, 2288, 2357, 2570, 2678
RseI CAYNNNNRTG 3 cut(s) 249, 660, 2722
SacI GAGCTC 3 cut(s) 310, 1011, 1065
SalI GTCGAC 1 cut(s) 273
SapI GCTCTTC 2 cut(s) 546, 1016
SatI GCNGC 6 cut(s) 615, 1810, 2327, 2330, 2548, 2551
Sau3AI GATC 5 cut(s) 1492, 1575, 2398, 2665, 2694
Sau96I GGNCC 5 cut(s) 19, 1220, 1634, 2514, 2578
SchI GAGTC 6 cut(s) 821, 1182, 1844, 1901, 2080, 2158
ScrFI CCNGG 4 cut(s) 453, 1638, 2189, 2531
SduI GDGCHC 3 cut(s) 310, 1011, 1065
SfaNI GCATC 5 cut(s) 130, 950, 1204, 1476, 1753
SfcI CTRYAG 2 cut(s) 69, 2324
SfuI TTCGAA 1 cut(s) 709
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 3 cut(s) 249, 660, 2722
SmlI CTYRAG 2 cut(s) 1064, 1786
SmoI CTYRAG 2 cut(s) 1064, 1786
SphI GCATGC 1 cut(s) 1438
SseBI AGGCCT 2 cut(s) 288, 1104
SspI AATATT 1 cut(s) 2008
SspMI CTAG 6 cut(s) 611, 836, 1727, 2180, 2340, 2746
SstI GAGCTC 3 cut(s) 310, 1011, 1065
StuI AGGCCT 2 cut(s) 288, 1104
StyD4I CCNGG 4 cut(s) 451, 1636, 2187, 2529
StyI CCWWGG 1 cut(s) 2291
TaaI ACNGT 6 cut(s) 404, 568, 676, 822, 2623, 2715
TaqI TCGA 8 cut(s) 228, 274, 304, 709, 855, 907, 1517, 1705
TatI WGTACW 3 cut(s) 793, 2356, 2677
TfiI GAWTC 7 cut(s) 46, 192, 391, 909, 1318, 1707, 2048
TscAI CASTG 1 cut(s) 2580
TseFI GTSAC 6 cut(s) 40, 1152, 1381, 1610, 1893, 2458
TseI GCWGC 6 cut(s) 614, 1809, 2326, 2329, 2547, 2550
Tsp45I GTSAC 6 cut(s) 40, 1152, 1381, 1610, 1893, 2458
TspGWI ACGGA 5 cut(s) 32, 71, 503, 1418, 1629
TspRI CASTG 1 cut(s) 2580
VpaK11BI GGWCC 1 cut(s) 19
VspI ATTAAT 1 cut(s) 2072
XapI RAATTY 5 cut(s) 233, 1535, 1960, 2113, 2207
XbaI TCTAGA 3 cut(s) 835, 1726, 2339
XceI RCATGY 6 cut(s) 250, 1373, 1438, 1684, 2543, 2705
XcmI CCANNNNNNNNNTGG 1 cut(s) 318
XmiI GTMKAC 2 cut(s) 274, 2272
XmnI GAANNNNTTC 6 cut(s) 128, 750, 1025, 1104, 2211, 2376
XspI CTAG 6 cut(s) 611, 836, 1727, 2180, 2340, 2746
Zsp2I ATGCAT 3 cut(s) 667, 1491, 2413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.