MD05G1024900.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000110
Physical Location & Seq
Forward (+)
4076687 .. 4080091
3405 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1024900.v1.1.491

Sequence Viewer

Length: 2838 bp
ATGGCTTCAGCTGCCGTAGACCTGCTGATCGGGAAAGTAGGATTGATCCTCGAAAGCGGTGTGTCTCAGTTAGAAGGAGTTGGTGATGAACTTCAACACCTCATGCGTGAGCTGCTAGCCATGAAATCCTTCTTGGAAGATGCCGAAAGAAGCGGAGCTTTAGGTGAAGTAGAGGAAACATGGATGGGAACTGTGAGGGACGTCTCCATCGATGTTCAAGACATCATTGATGATTTCACATATCACAGAAACATGGAGAAATGTTGGGGTCCATACACAAGAGCCTTTCATCAGACAATTTTCTTCCCCATGAATCTTTGGAAGAGGCATCGCATCGCCACCAAATTGCACAAGATCATCATCAAAGCAATCAAAGCTATTCCGGAAAGAAACCAACTTCGTGGTGTTGATCGTAGCCAAGGCGGCCGTGATTGCGATCACGGCCAAATCAATATCTACGGTGAGTCCTCTCTTTTCTTCAAAGATGATGAACTTGTGGGAATCAAAGATGCTAAAGAAAAGTTAGTTGGATGGTTGCTGTCTGGAGAACCTCAGAGGATCGTAATTTCCGTAGTTGGAATGGGGGGTTCGGGTAAGACAAGCTTGGTTGCGGACACCTTCAACAGTCAAACTGCCAAGTTTGATTGCTACGCGTGGATCACAGTCTCGAAAACTTACAACATCGAATGTCTATTGAGAGTTTTGATAAAAGAACTTTTTGCATCTGCAATGGAACGTGTTCCTCAAGACTTGAGCAGGATGAGCCAAATACAAATGGTGGAATTGCTGGCCAACTATTTGCAGCCAAAGAGGTATGTCATTGTGTTTGACGATGTGTGGGATACTAATCTTTGGAGACAGATAAATGGAGCACTTCCAGATGGAACACATGGAAGCCGGGTCATGCTTACAACTCGGAGAGAAGACATTGCATCCTTTTCATTTGGATTTGGATGCCATATTCATCATGTTCAGCCTCTTAAGGAGAATGAGGCTTGGGTTCTTTTTTCCAAGAAAGCTTTCACTGGCCTGCCTGACAATTGTTGCCCACCAGAGTTTGAACACATTGCTAGAGATCTTGTTGGAAAATGCAAAGGATTACCTCTTGGAATTGCGGCTTTGGGTGCTCTCATGTCAACCAAAAGATTGGCGTCTGAGTGGACAAACTTTTACACTACTTTTAATTGGGAGTTAACCAACAACCCAATGCTTGAAGTAGTAAAGAGAATCTTGATGCTAAGCTATAATGATTTACCTTACCGACTCGAGCATTGTTTCTTATACATGTGCATTTTCCCAGAAGATTATGTGATCGAATGTGACAGGCTTGTTAGACTATGGATGGCAGAGGGGTTTGTGGAAAAAGTTCGATGGGCCAAGCCAGAAGAGCTGGCTGAAAGCTACATAGCAGAGCTCACTTGCCGATGCATGGTACAAGTAGTTAAGAGGGAACCATATGGAAGGGCAAAAGCTTTAAAGATGCATGACCTCTTACTAGAACTTGCTCTTTCAATTTCCGAAGTTGAGAAATTCTGCACCGTCTATAATGAGCAAAAAAAAAATGAAGATAAAAGGACTCCCTATCGGCTCTCAGTGCAAGCAAAGTATGGAAAACAAGCCCACAGAGACATGTCAAGGGTTCGCACATTCTTTATTTTTGCTCCTAATATGACTGATTCTTCTTCAGTTGAAAAGTTGCCATCTGATTTCAAACTCTTGAAGGTAATGGATCTTAGACATGTCCCGATTACTAAACTCCCAGATCAATTAGTGAAGTTCTATAACATGAAGTATTTAAATTTGAAAGGAATGCAAGTGAAAGAACTTCCAAGGGACATAGGGAATCTTCATCATTTGGAAACCCTTGACATTAGGCATTCCAAAATCAGGATGCTTCCAGCAGGAGTTGTGAAGTTACAAAACTTACGGCATCTGTTGATGTATCATTGCAATTTTCAGGATCTTTTTAGGTCTTATTATTTCTTTGATGGCACAAAAGTACCTCAAGGCATATGTAAGCTAGAGAGCTTACAAGTTTGTGATGCTATCGAGTTAGAAGGCACGCTAGTCAAACAACTTCGACAGTTTACCCAACTCACAAGAATGAGCATAACCAATGTGAGAGAGTCAGATGAGGAGGACTTGTGCAAGACACTTGAAAGCATGAAACTCATAGAGCACTTCTTTGTCCATACAAGTAATGAAGATGAGGTTTTACGCTTGGATGCATTGCCATCAGCACCTCCTTTGCTGAAGGCACTTGGTCTAATTGGAAAATTAGAGAAGGTTCCACTTTGGTTTCATTCTCTTTTCAGCCTTACACAATTAAGGTTGCATTGGTCCAGATCAACAAAAGATTTTCTTCCTTACATCCAAACACTACCTAATTTGAGCATACTTCGGCTGAACAATGCATATCTCGGAAATCAATTGGTCTTTCACAGTGGATTTCCAAAGCTGTCTGAATTATACATAATGGAGTTGCCTAAATTGAATGCGATCATCATAGAGAGAGGAGCGATGCCAACACTACAAAGACTTGTAATCACTGAATGCATGGAGTTAAAGCAATTGCCTAATGGAATCGATCATCTTACATGTCTTCAAAGTTTGAGTTTGGTAACTGTTCCCCATGAGCTCGTAGAGCGAATACGCAATGAAGGAAGCTTGGATCGTTCCAAGGTTATGCACATCTCAGACATCAGTTATCACTACAAAACTGAACTCGGGTGGTCTGGGGAAAGATTGAGATCATCTCTACAATGGGTCGTTCAACCCTCAAATACTCCGGTCAGCATTCTATCCCGAGATGGTCTACCTATTTTACCAGGTAATTAA

Protein Analysis

946

Amino Acids

108.55

Weight (kDa)

7.56

Isoelectric Point (pI)

46.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 88 1.8e-17 Rx N-terminal domain
NB-ARC PF00931 169 - 342 9.5e-43 NB-ARC domain
WHD_DRP PF23559 431 - 502 2.6e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 547 - 873 1.9e-42 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 204
Acc36I ACCTGC 1 cut(s) 30
AccI GTMKAC 2 cut(s) 18, 2815
AccII CGCG 1 cut(s) 653
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 5 cut(s) 57, 153, 423, 611, 1115
AclWI GGATC 6 cut(s) 40, 566, 665, 1737, 1968, 2679
AcoI YGGCCR 3 cut(s) 424, 442, 789
AcsI RAATTY 2 cut(s) 1529, 1798
AcuI CTGAAG 2 cut(s) 1668, 2273
AcyI GRCGYC 2 cut(s) 201, 1151
AfaI GTAC 2 cut(s) 1434, 2001
AfiI CCNNNNNNNGG 2 cut(s) 1429, 1887
AflII CTTAAG 1 cut(s) 980
AflIII ACRYGT 6 cut(s) 651, 736, 1284, 1629, 1738, 2597
AjnI CCWGG 1 cut(s) 2827
AjuI GAANNNNNNNTTGG 6 cut(s) 116, 148, 510, 542, 1004, 1036
Alw21I GWGCWC 5 cut(s) 874, 1129, 1416, 2181, 2640
Alw26I GTCTC 5 cut(s) 69, 208, 670, 850, 1620
AlwI GGATC 6 cut(s) 40, 566, 665, 1737, 1968, 2679
Ama87I CYCGRG 3 cut(s) 1265, 2726, 2805
Aor13HI TCCGGA 1 cut(s) 382
AoxI GGCC 5 cut(s) 424, 442, 789, 1027, 1374
ApeKI GCWGC 3 cut(s) 11, 112, 802
ApoI RAATTY 2 cut(s) 1529, 1798
Asp700I GAANNNNTTC 4 cut(s) 128, 738, 1019, 1365
AspS9I GGNCC 3 cut(s) 269, 1374, 2340
AsuC2I CCSGG 1 cut(s) 899
AsuHPI GGTGA 3 cut(s) 95, 176, 473
AsuNHI GCTAGC 1 cut(s) 115
AvaI CYCGRG 3 cut(s) 1265, 2726, 2805
AvaII GGWCC 2 cut(s) 269, 2340
BaeI ACNNNNGTAYC 2 cut(s) 1983, 2016
BalI TGGCCA 1 cut(s) 791
BanII GRGCYC 2 cut(s) 1416, 2640
BbsI GAAGAC 2 cut(s) 930, 2594
Bbv12I GWGCWC 5 cut(s) 874, 1129, 1416, 2181, 2640
BbvI GCAGC 2 cut(s) 99, 814
BceAI ACGGC 3 cut(s) 411, 457, 1943
BciT130I CCWGG 1 cut(s) 2829
BciVI GTATCC 1 cut(s) 835
BcnI CCSGG 1 cut(s) 899
BcoDI GTCTC 5 cut(s) 69, 208, 670, 850, 1620
BfaI CTAG 5 cut(s) 116, 1071, 1496, 2021, 2066
BfrI CTTAAG 1 cut(s) 980
BfuAI ACCTGC 1 cut(s) 30
BfuI GTATCC 1 cut(s) 835
BglI GCCNNNNNGGC 1 cut(s) 423
BglII AGATCT 1 cut(s) 1075
BisI GCNGC 5 cut(s) 12, 113, 424, 803, 1116
BlpI GCTNAGC 1 cut(s) 1238
BlsI GCNGC 5 cut(s) 13, 114, 425, 804, 1117
Bme1390I CCNGG 2 cut(s) 899, 2829
Bme18I GGWCC 2 cut(s) 269, 2340
BmeT110I CYCGRG 3 cut(s) 1265, 2726, 2805
BmgT120I GGNCC 3 cut(s) 269, 1374, 2340
BmiI GGNNCC 3 cut(s) 270, 1452, 2289
BmrFI CCNGG 2 cut(s) 899, 2829
BmtI GCTAGC 1 cut(s) 119
BpiI GAAGAC 2 cut(s) 930, 2594
BplI GAGNNNNNCTC 2 cut(s) 2740, 2772
BpmI CTGGAG 1 cut(s) 564
Bpu1102I GCTNAGC 1 cut(s) 1238
BpuEI CTTGAG 3 cut(s) 729, 772, 1989
BpuMI CCSGG 1 cut(s) 899
Bsa29I ATCGAT 2 cut(s) 210, 2586
BsaBI GATNNNNATC 4 cut(s) 359, 435, 846, 2749
BsaHI GRCGYC 2 cut(s) 201, 1151
BsaJI CCNNGG 3 cut(s) 418, 1829, 2679
BsaWI WCCGGW 2 cut(s) 382, 2788
BsaXI ACNNNNNCTCC 2 cut(s) 2129, 2159
Bsc4I CCNNNNNNNGG 2 cut(s) 1429, 1887
Bse1I ACTGG 1 cut(s) 1030
Bse3DI GCAATG 6 cut(s) 735, 927, 1065, 1945, 2228, 2662
Bse8I GATNNNNATC 4 cut(s) 359, 435, 846, 2749
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 1 cut(s) 2829
BseCI ATCGAT 2 cut(s) 210, 2586
BseDI CCNNGG 3 cut(s) 418, 1829, 2679
BseGI GGATG 9 cut(s) 189, 536, 765, 932, 959, 1347, 1896, 2230, 2370
BseJI GATNNNNATC 4 cut(s) 359, 435, 846, 2749
BseLI CCNNNNNNNGG 2 cut(s) 1429, 1887
BseMI GCAATG 6 cut(s) 735, 927, 1065, 1945, 2228, 2662
BseMII CTCAG 5 cut(s) 80, 566, 1146, 1605, 2709
BseNI ACTGG 1 cut(s) 1030
BseRI GAGGAG 2 cut(s) 2150, 2529
BseX3I CGGCCG 1 cut(s) 424
BseXI GCAGC 2 cut(s) 99, 814
BsgI GTGCAG 1 cut(s) 1519
Bsh1236I CGCG 1 cut(s) 653
Bsh1285I CGRYCG 1 cut(s) 427
BshFI GGCC 5 cut(s) 426, 444, 791, 1029, 1376
BshVI ATCGAT 2 cut(s) 210, 2586
BsiEI CGRYCG 1 cut(s) 427
BsiHKAI GWGCWC 5 cut(s) 874, 1129, 1416, 2181, 2640
BsiHKCI CYCGRG 3 cut(s) 1265, 2726, 2805
BsiSI CCGG 3 cut(s) 383, 898, 2789
BslFI GGGAC 3 cut(s) 212, 1727, 1847
BslI CCNNNNNNNGG 2 cut(s) 1429, 1887
BsmAI GTCTC 5 cut(s) 69, 208, 670, 850, 1620
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 3 cut(s) 212, 1727, 1847
BsmI GAATGC 5 cut(s) 1815, 1876, 2500, 2558, 2796
BsnI GGCC 5 cut(s) 426, 444, 791, 1029, 1376
BsoBI CYCGRG 3 cut(s) 1265, 2726, 2805
Bsp1286I GDGCHC 5 cut(s) 874, 1129, 1416, 2181, 2640
Bsp13I TCCGGA 1 cut(s) 382
Bsp1720I GCTNAGC 1 cut(s) 1238
BspACI CCGC 5 cut(s) 57, 153, 423, 611, 1115
BspANI GGCC 5 cut(s) 426, 444, 791, 1029, 1376
BspCNI CTCAG 5 cut(s) 79, 565, 1147, 1604, 2708
BspDI ATCGAT 2 cut(s) 210, 2586
BspEI TCCGGA 1 cut(s) 382
BspFNI CGCG 1 cut(s) 653
BspLI GGNNCC 3 cut(s) 270, 1452, 2289
BspMI ACCTGC 1 cut(s) 30
BspOI GCTAGC 1 cut(s) 119
BspPI GGATC 6 cut(s) 40, 566, 665, 1737, 1968, 2679
BspQI GCTCTTC 1 cut(s) 1380
BspTI CTTAAG 1 cut(s) 980
BsrDI GCAATG 6 cut(s) 735, 927, 1065, 1945, 2228, 2662
BsrI ACTGG 1 cut(s) 1030
BssECI CCNNGG 3 cut(s) 418, 1829, 2679
BssNI GRCGYC 2 cut(s) 201, 1151
BssT1I CCWWGG 3 cut(s) 418, 1829, 2679
Bst2UI CCWGG 1 cut(s) 2829
Bst4CI ACNGT 8 cut(s) 193, 461, 626, 664, 1540, 2085, 2444, 2626
Bst6I CTCTTC 2 cut(s) 317, 1380
BstACI GRCGYC 2 cut(s) 201, 1151
BstAFI CTTAAG 1 cut(s) 980
BstC8I GCNNGC 6 cut(s) 117, 789, 1031, 1392, 1599, 2063
BstDEI CTNAG 7 cut(s) 66, 552, 1155, 1238, 1591, 1733, 2695
BstF5I GGATG 9 cut(s) 189, 536, 765, 932, 959, 1347, 1896, 2230, 2370
BstFNI CGCG 1 cut(s) 653
BstMAI GTCTC 5 cut(s) 69, 208, 670, 850, 1620
BstMCI CGRYCG 1 cut(s) 427
BstNI CCWGG 1 cut(s) 2829
BstNSI RCATGY 4 cut(s) 1288, 1633, 1742, 2601
BstSCI CCNGG 2 cut(s) 897, 2827
BstUI CGCG 1 cut(s) 653
BstV1I GCAGC 2 cut(s) 99, 814
BstV2I GAAGAC 2 cut(s) 930, 2594
BstX2I RGATCY 3 cut(s) 1075, 1729, 1960
BstXI CCANNNNNNTGG 2 cut(s) 401, 1147
BstYI RGATCY 3 cut(s) 1075, 1729, 1960
BstZI CGGCCG 1 cut(s) 424
Bsu15I ATCGAT 2 cut(s) 210, 2586
BsuI GTATCC 1 cut(s) 835
BsuRI GGCC 5 cut(s) 426, 444, 791, 1029, 1376
BsuTUI ATCGAT 2 cut(s) 210, 2586
BtgZI GCGATG 3 cut(s) 314, 319, 2534
BtsCI GGATG 9 cut(s) 189, 536, 765, 932, 959, 1347, 1896, 2230, 2370
BtsIMutI CAGTG 4 cut(s) 1023, 1599, 2449, 2547
BveI ACCTGC 1 cut(s) 30
Cac8I GCNNGC 6 cut(s) 117, 789, 1031, 1392, 1599, 2063
Cfr13I GGNCC 3 cut(s) 269, 1374, 2340
ClaI ATCGAT 2 cut(s) 210, 2586
CseI GACGC 1 cut(s) 1140
CsiI ACCWGGT 1 cut(s) 2827
Csp6I GTAC 2 cut(s) 1433, 2000
CviQI GTAC 2 cut(s) 1433, 2000
DdeI CTNAG 7 cut(s) 66, 552, 1155, 1238, 1591, 1733, 2695
DraI TTTAAA 2 cut(s) 1476, 1797
EaeI YGGCCR 3 cut(s) 424, 442, 789
EagI CGGCCG 1 cut(s) 424
Eam1104I CTCTTC 2 cut(s) 317, 1380
EarI CTCTTC 2 cut(s) 317, 1380
Ecl136II GAGCTC 2 cut(s) 1414, 2638
EclXI CGGCCG 1 cut(s) 424
Eco130I CCWWGG 3 cut(s) 418, 1829, 2679
Eco24I GRGCYC 2 cut(s) 1416, 2640
Eco47I GGWCC 2 cut(s) 269, 2340
Eco52I CGGCCG 1 cut(s) 424
Eco53kI GAGCTC 2 cut(s) 1414, 2638
Eco57I CTGAAG 2 cut(s) 1668, 2273
Eco88I CYCGRG 3 cut(s) 1265, 2726, 2805
EcoICRI GAGCTC 2 cut(s) 1414, 2638
EcoRII CCWGG 1 cut(s) 2827
EcoT14I CCWWGG 3 cut(s) 418, 1829, 2679
EcoT22I ATGCAT 5 cut(s) 1430, 1485, 2230, 2416, 2558
EcoT38I GRGCYC 2 cut(s) 1416, 2640
ErhI CCWWGG 3 cut(s) 418, 1829, 2679
Esp3I CGTCTC 1 cut(s) 208
FalI AAGNNNNNCTT 8 cut(s) 142, 174, 587, 619, 1214, 1246, 2346, 2378
FaqI GGGAC 3 cut(s) 212, 1727, 1847
FauNDI CATATG 2 cut(s) 1456, 2012
FblI GTMKAC 2 cut(s) 18, 2815
Fnu4HI GCNGC 5 cut(s) 12, 113, 424, 803, 1116
FokI GGATG 9 cut(s) 196, 543, 772, 919, 966, 1354, 1903, 2237, 2357
FriOI GRGCYC 2 cut(s) 1416, 2640
Fsp4HI GCNGC 5 cut(s) 12, 113, 424, 803, 1116
FspBI CTAG 5 cut(s) 116, 1071, 1496, 2021, 2066
GluI GCNGC 5 cut(s) 12, 113, 424, 803, 1116
GsuI CTGGAG 1 cut(s) 564
HaeIII GGCC 5 cut(s) 426, 444, 791, 1029, 1376
HapII CCGG 3 cut(s) 383, 898, 2789
HgaI GACGC 1 cut(s) 1140
Hin1I GRCGYC 2 cut(s) 201, 1151
HincII GTYRAC 2 cut(s) 1137, 1194
HindII GTYRAC 2 cut(s) 1137, 1194
HindIII AAGCTT 4 cut(s) 601, 1017, 1470, 2665
HpaI GTTAAC 1 cut(s) 1194
HpaII CCGG 3 cut(s) 383, 898, 2789
HphI GGTGA 3 cut(s) 95, 176, 473
Hpy166II GTNNAC 6 cut(s) 19, 1137, 1161, 1194, 2088, 2816
Hpy8I GTNNAC 6 cut(s) 19, 1137, 1161, 1194, 2088, 2816
HpyAV CCTTC 9 cut(s) 68, 139, 628, 1455, 1714, 2051, 2248, 2278, 2654
HpyCH4III ACNGT 8 cut(s) 193, 461, 626, 664, 1540, 2085, 2444, 2626
HpyCH4IV ACGT 2 cut(s) 201, 736
HpyF3I CTNAG 7 cut(s) 66, 552, 1155, 1238, 1591, 1733, 2695
HpySE526I ACGT 2 cut(s) 201, 736
Hsp92I GRCGYC 2 cut(s) 201, 1151
Kpn2I TCCGGA 1 cut(s) 382
KspAI GTTAAC 1 cut(s) 1194
LguI GCTCTTC 1 cut(s) 1380
LmnI GCTCC 4 cut(s) 155, 869, 1666, 2516
Lsp1109I GCAGC 2 cut(s) 99, 814
MabI ACCWGGT 1 cut(s) 2827
MaeI CTAG 5 cut(s) 116, 1071, 1496, 2021, 2066
MaeII ACGT 2 cut(s) 201, 736
MaeIII GTNAC 3 cut(s) 1319, 1914, 2620
MfeI CAATTG 3 cut(s) 1039, 2429, 2570
MflI RGATCY 3 cut(s) 1075, 1729, 1960
MhlI GDGCHC 5 cut(s) 874, 1129, 1416, 2181, 2640
MlsI TGGCCA 1 cut(s) 791
MluI ACGCGT 1 cut(s) 651
MluNI TGGCCA 1 cut(s) 791
MlyI GAGTC 4 cut(s) 473, 1257, 1570, 2135
MmeI TCCRAC 3 cut(s) 508, 556, 1063
Mox20I TGGCCA 1 cut(s) 791
Mph1103I ATGCAT 5 cut(s) 1430, 1485, 2230, 2416, 2558
MroI TCCGGA 1 cut(s) 382
MroXI GAANNNNTTC 4 cut(s) 128, 738, 1019, 1365
MscI TGGCCA 1 cut(s) 791
MseI TTAA 9 cut(s) 981, 1182, 1193, 1443, 1475, 1796, 2327, 2564, 2836
MslI CAYNNNNRTG 1 cut(s) 2102
Msp20I TGGCCA 1 cut(s) 791
MspA1I CMGCKG 1 cut(s) 11
MspCI CTTAAG 1 cut(s) 980
MspI CCGG 3 cut(s) 383, 898, 2789
MspR9I CCNGG 2 cut(s) 899, 2829
MunI CAATTG 3 cut(s) 1039, 2429, 2570
Mva1269I GAATGC 5 cut(s) 1815, 1876, 2500, 2558, 2796
MvaI CCWGG 1 cut(s) 2829
MvnI CGCG 1 cut(s) 653
NciI CCSGG 1 cut(s) 899
NdeI CATATG 2 cut(s) 1456, 2012
NheI GCTAGC 1 cut(s) 115
NlaIV GGNNCC 3 cut(s) 270, 1452, 2289
NmuCI GTSAC 1 cut(s) 1319
NsiI ATGCAT 5 cut(s) 1430, 1485, 2230, 2416, 2558
NspI RCATGY 4 cut(s) 1288, 1633, 1742, 2601
PaeR7I CTCGAG 1 cut(s) 1265
PciI ACATGT 4 cut(s) 1284, 1629, 1738, 2597
PciSI GCTCTTC 1 cut(s) 1380
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 567
PctI GAATGC 5 cut(s) 1815, 1876, 2500, 2558, 2796
PdmI GAANNNNTTC 4 cut(s) 128, 738, 1019, 1365
PfeI GAWTC 6 cut(s) 313, 501, 1227, 1676, 1843, 2583
PkrI GCNGC 5 cut(s) 13, 114, 425, 804, 1117
PleI GAGTC 4 cut(s) 472, 1257, 1570, 2134
PpsI GAGTC 4 cut(s) 472, 1257, 1570, 2134
PscI ACATGT 4 cut(s) 1284, 1629, 1738, 2597
Psp124BI GAGCTC 2 cut(s) 1416, 2640
Psp6I CCWGG 1 cut(s) 2827
PspGI CCWGG 1 cut(s) 2827
PspN4I GGNNCC 3 cut(s) 270, 1452, 2289
PspPI GGNCC 3 cut(s) 269, 1374, 2340
PspXI VCTCGAGB 1 cut(s) 1265
PsuI RGATCY 3 cut(s) 1075, 1729, 1960
PvuII CAGCTG 1 cut(s) 11
RsaI GTAC 2 cut(s) 1434, 2001
RsaNI GTAC 2 cut(s) 1433, 2000
RseI CAYNNNNRTG 1 cut(s) 2102
SacI GAGCTC 2 cut(s) 1416, 2640
SapI GCTCTTC 1 cut(s) 1380
SaqAI TTAA 9 cut(s) 981, 1182, 1193, 1443, 1475, 1796, 2327, 2564, 2836
SatI GCNGC 5 cut(s) 12, 113, 424, 803, 1116
Sau96I GGNCC 3 cut(s) 269, 1374, 2340
SchI GAGTC 4 cut(s) 473, 1257, 1570, 2135
ScrFI CCNGG 2 cut(s) 899, 2829
SduI GDGCHC 5 cut(s) 874, 1129, 1416, 2181, 2640
SexAI ACCWGGT 1 cut(s) 2827
Sfr274I CTCGAG 1 cut(s) 1265
SinI GGWCC 2 cut(s) 269, 2340
SlaI CTCGAG 1 cut(s) 1265
SmiI ATTTAAAT 1 cut(s) 1797
SmiMI CAYNNNNRTG 1 cut(s) 2102
SmlI CTYRAG 5 cut(s) 744, 751, 980, 1265, 2004
SmoI CTYRAG 5 cut(s) 744, 751, 980, 1265, 2004
SsiI CCGC 5 cut(s) 57, 153, 423, 611, 1115
SspMI CTAG 5 cut(s) 116, 1071, 1496, 2021, 2066
SstI GAGCTC 2 cut(s) 1416, 2640
StyD4I CCNGG 2 cut(s) 897, 2827
StyI CCWWGG 3 cut(s) 418, 1829, 2679
SwaI ATTTAAAT 1 cut(s) 1797
TaaI ACNGT 8 cut(s) 193, 461, 626, 664, 1540, 2085, 2444, 2626
TaiI ACGT 2 cut(s) 204, 739
TauI GCSGC 2 cut(s) 426, 1118
TfiI GAWTC 6 cut(s) 313, 501, 1227, 1676, 1843, 2583
Tru1I TTAA 9 cut(s) 981, 1182, 1193, 1443, 1475, 1796, 2327, 2564, 2836
Tru9I TTAA 9 cut(s) 981, 1182, 1193, 1443, 1475, 1796, 2327, 2564, 2836
TscAI CASTG 4 cut(s) 1030, 1599, 2449, 2554
TseFI GTSAC 1 cut(s) 1319
TseI GCWGC 3 cut(s) 11, 112, 802
Tsp45I GTSAC 1 cut(s) 1319
TspGWI ACGGA 1 cut(s) 559
TspRI CASTG 4 cut(s) 1030, 1599, 2449, 2554
Vha464I CTTAAG 1 cut(s) 980
VpaK11BI GGWCC 2 cut(s) 269, 2340
XapI RAATTY 2 cut(s) 1529, 1798
XceI RCATGY 4 cut(s) 1288, 1633, 1742, 2601
XhoI CTCGAG 1 cut(s) 1265
XmiI GTMKAC 2 cut(s) 18, 2815
XmnI GAANNNNTTC 4 cut(s) 128, 738, 1019, 1365
XspI CTAG 5 cut(s) 116, 1071, 1496, 2021, 2066
ZraI GACGTC 1 cut(s) 202
Zsp2I ATGCAT 5 cut(s) 1430, 1485, 2230, 2416, 2558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.