RchiOBHm_Chr2g0101551

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000110
Physical Location & Seq
Reverse (-)
13144469 .. 13147232
2764 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47615

Sequence Viewer

Length: 2748 bp
ATGGCCTCAGCTCCAACAGACCTCTTGATTGGCAAAATTGTGAGCATTCTAGAGAGCGAAGCATCCTCCATAGCCGGTGTTCGTGATGAAGTTGATGAGATTAAGCTGGAGCTTGAGAGCATGAAGTCTTTCCTAGAGGATACAGAGCGGAAGAAAGCAAGTACACAAGGAGAGAAAACGTGGATTGCAAACGTGAGAAACTTGGCCTGCGATGTTGAAGATATCATTGATGAATTCATGTATCGCATGTATGAGAAAGAAAGTGGCGGTCGATTTGCAAGGTTTCTCCAGAAAACCATTCATGTCCCAAAAAACCTCTGGTATAAGCGTCGAATTGCTAACAAGTTACAGAAAATTATTGCAGCAATCAAAGCCATTCCAGAGAGAAAACAGAGGTATGGTCTAATTGGTCAAGAAGGAACATCTTCTGATGATGTTCACAAGAGGGTAAAAACCAAAGCCGAGTCCTCGCTTTTTGAGAAGGAAGATGAACTCGTGGGAATTGAAGGGAAGAAGCAAATGTTAATGGGATGGTTGATCAATGGAGAGAAAAAACAAACTATTGTCTCTGTGGTCGGCATGGGAGGATCAGGGAAGACAACTCTCGTTGCCAACACCTTCACTCAGGAGACAGTAAAGAGACATTTTAATTGTTTTGCATGGATAACTGTTTCCCAAACTTATGTGCTTGAAGACTTATTTAGAAGCTTGATAAAGGAATTCCACCAATCAATAAAACAGGAAGTCCCTGGGAATTTGCATGCAATGAGCTACAGGGAATTGCTGGACATGTTGACAAACTTTTTGGTGTCCAAAAGATATCTAGTTGTATTGGATGACGTGTGGGATATCACTCTTTGGGAAAATATAAGATTATCATTTCCAGATGAACTGATTGGAAGTCGAATCATTCTTACAACTCGAAGAGAAGACATAGCATCCTATTCTTTTGGAGTTGAAAGTCATGTTTACCCTATTCAACTCTTGCAACGCGATGAGGCCAAGGAGCTCTTCAGCAAGAAAGCATTCCCAACTTATGACAATATATGCCCACCAGAGCTTGAACCCTTAGCTTGGGAACTTGTGGAGAAGTGTAACGGCTTACCTCTGGCAATTGTGGCTTTACGTGGCCTCATGTCCTCAAAAAAGTCTTCTGTAGAGTGGAGTACAGTCCTCAACAGCTTAAATTGGCATCTAAGTAACAATCCGTTGTTAGAATCTATAAAGATCATCTTGTTGCTTAGTTTCAACGGTTTGTCATATCGATTAAAACGTTGTTTCCTATATTGTTCCTTATTCCCAGAGGATTACTTGATGGGGAAAAATAGACTCATTAGACTGTGGATAGCTGAAGGATTTATTGAACACGTTAAAGGGGTTACACCAGAAGCAGTTGCAGAACTCTATATTATGGAACTCGTTTCTCGTAGCATGCTACAAGCTGTAAGAAGAAATGAAACCGGAAGGCCAAGGGCATGCAAGATGCATGATCTTATGCGGGAGCTTGCTTTATCAGAATCAGCATCGGAGAACTTTGCCACTGTGTACGATGGCAAAGAAGTAATGAAAGAAATGGGAGCCCGTCGTTTGTCAATTCAAACAACCGATGGGGAAATTAAGCCACTCACAGATATGTCACATCTTCGTTCTTTTCTTGTCTTTGTCACTAATAGAATCTCATCGTCTGTTTCTGAGATATTGCCTTCAGGATTGAAGTTGTTGAGGATTCTAGATTTGGAACGGTCCCCGGTTACTAAATTGCCAGATGAAGTTGTGTACTTATTCAACTTAAGATATTTAAATTTGAGGAAAACTCCCATTAAAGAGCTTCCCAAATCCATAGGACGGCTCCACAATCTCCAGACCTTGGACATAAGGGATTCCAATATAAAGGCACTTCCAAGGGGAATTACCAAGCTGCTTAACCTACGCCATCTGATTATGTACCGTTACACTGGAGTACATAAGGGCTTCAGATATATTGAAGGGACAAAAGGTCCATCTGGTATTTGTAAATTGAAGAACTTGCAAGTTTTGGCATGTGTTGAATCAGAAGGAAATGTTATTAGACTTGTCGGGAACATGACCCAACTTACTAAGCTTGGCATCACAAATGTAAAAGAAAGAGACGAGATAGACCTATGTGCTTCAATTCAAAAGATGGAGCTCCTCCAGGACTTGTTTTTAATGGTACCTGATGAAGAGGAATGTCTTCGACTCGATGCGCTAACTGCACCTCCTGCTCACCTTCACAAACTTGCGTTGGCTGGAAAGTTGGAAATGGTACCACGTTGGTTTTTTTCACTTCACAGCCTCACATCTTTGAATCTGCATTGGTCTAGACTCGAGGAAGATTTATTACCTCACATTGAAGCACTGGCCAGCCTACGAAGCCTTACTCTTTATAATGCATACACTGGGGAAAACCTGAGTTTCAGTGCAGGCTTCAGAAATCTTGTGCATTTGCAGCTTTGCAATTTCCCTTTACTGAATAACATAACTATAGAGAGAGGGGTGATGCCAAAGCTGCAGTTCTTGATGCTGGATAGCTGTCTGGAAGTAAAGACTTTGCCGTGGGGTATTGAATACCTTTCTAATCTTGAAACCTTGCAGTTGGGATCTGTTTCAGTGCAACTTCTAGAGTCCATACAAGAAGGAGGTGTGGATCATTCAAAGGTACGACACCTTCCAGAAGTAGATGATTTAGTCAGAACGTTATTTGGGAGGTTTAATGAAAGCAAGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

915

Amino Acids

104.35

Weight (kDa)

7.85

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 90 2.3e-22 Rx N-terminal domain
NB-ARC PF00931 170 - 345 6.1e-44 NB-ARC domain
WHD_DRP PF23559 432 - 503 2.5e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 547 - 871 1.2e-43 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2406
Acc65I GGTACC 2 cut(s) 2191, 2284
AccB1I GGYRCC 2 cut(s) 2191, 2284
AccB7I CCANNNNNTGG 1 cut(s) 1867
AccBSI CCGCTC 1 cut(s) 148
AccII CGCG 1 cut(s) 993
AciI CCGC 3 cut(s) 148, 267, 1498
AclI AACGTT 2 cut(s) 1273, 2714
AclWI GGATC 3 cut(s) 595, 2626, 2673
AcoI YGGCCR 1 cut(s) 2379
AcsI RAATTY 4 cut(s) 233, 719, 754, 1801
AcuI CTGAAG 5 cut(s) 997, 1371, 1689, 1957, 2431
AfaI GTAC 9 cut(s) 163, 1168, 1547, 1778, 1946, 1962, 2193, 2286, 2679
AfiI CCNNNNNNNGG 3 cut(s) 1074, 1845, 1867
AflII CTTAAG 1 cut(s) 1789
AflIII ACRYGT 3 cut(s) 789, 840, 1366
AhdI GACNNNNNGTC 1 cut(s) 1995
AjiI CACGTC 1 cut(s) 841
AjnI CCWGG 2 cut(s) 748, 2172
AjuI GAANNNNNNNTTGG 2 cut(s) 995, 1027
Alw21I GWGCWC 2 cut(s) 1011, 2169
Alw26I GTCTC 4 cut(s) 571, 623, 634, 2121
AlwI GGATC 3 cut(s) 595, 2626, 2673
Ama87I CYCGRG 1 cut(s) 2345
AoxI GGCC 6 cut(s) 3, 204, 999, 1129, 1466, 2379
ApeKI GCWGC 4 cut(s) 362, 1918, 2467, 2527
ApoI RAATTY 4 cut(s) 233, 719, 754, 1801
ArsI GACNNNNNNTTYG 2 cut(s) 787, 819
Asp700I GAANNNNTTC 4 cut(s) 128, 424, 1025, 2211
Asp718I GGTACC 2 cut(s) 2191, 2284
AspLEI GCGC 1 cut(s) 2227
AspS9I GGNCC 2 cut(s) 1743, 1997
AsuC2I CCSGG 1 cut(s) 1748
AsuHPI GGTGA 2 cut(s) 2237, 2527
AvaI CYCGRG 1 cut(s) 2345
AvaII GGWCC 2 cut(s) 1743, 1997
BalI TGGCCA 1 cut(s) 2381
BanI GGYRCC 2 cut(s) 2191, 2284
BanII GRGCYC 3 cut(s) 1011, 1582, 2169
BarI GAAGNNNNNNTAC 4 cut(s) 2343, 2375, 2670, 2702
BauI CACGAG 1 cut(s) 494
BbsI GAAGAC 5 cut(s) 602, 699, 936, 1143, 2204
Bbv12I GWGCWC 2 cut(s) 1011, 2169
BbvCI CCTCAGC 1 cut(s) 7
BbvI GCAGC 4 cut(s) 374, 1905, 2479, 2514
BccI CCATC 7 cut(s) 525, 1309, 1544, 1601, 1941, 2008, 2155
BceAI ACGGC 3 cut(s) 1114, 1862, 2557
BciT130I CCWGG 2 cut(s) 750, 2174
BciVI GTATCC 1 cut(s) 133
BclI TGATCA 1 cut(s) 537
BcnI CCSGG 1 cut(s) 1748
BcoDI GTCTC 4 cut(s) 571, 623, 634, 2121
BfaI CTAG 7 cut(s) 50, 134, 824, 1730, 2340, 2639, 2746
BfmI CTRYAG 4 cut(s) 772, 1155, 2502, 2528
BfrI CTTAAG 1 cut(s) 1789
BfuI GTATCC 1 cut(s) 133
BisI GCNGC 4 cut(s) 363, 1919, 2468, 2528
BlsI GCNGC 4 cut(s) 364, 1920, 2469, 2529
Bme1390I CCNGG 3 cut(s) 750, 1748, 2174
Bme18I GGWCC 2 cut(s) 1743, 1997
BmeRI GACNNNNNGTC 1 cut(s) 1995
BmeT110I CYCGRG 1 cut(s) 2345
BmgBI CACGTC 1 cut(s) 841
BmgT120I GGNCC 2 cut(s) 1743, 1997
BmiI GGNNCC 5 cut(s) 1579, 1745, 1850, 2193, 2286
BmrFI CCNGG 3 cut(s) 750, 1748, 2174
BmrI ACTGGG 1 cut(s) 2427
BmsI GCATC 9 cut(s) 71, 947, 1201, 1473, 1532, 2115, 2212, 2508, 2529
BmuI ACTGGG 1 cut(s) 2427
BpiI GAAGAC 5 cut(s) 602, 699, 936, 1143, 2204
BplI GAGNNNNNCTC 2 cut(s) 1798, 1830
BpmI CTGGAG 5 cut(s) 128, 272, 1844, 1977, 2156
Bpu10I CCTNAGC 2 cut(s) 7, 1069
BpuEI CTTGAG 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 1748
Bsa29I ATCGAT 1 cut(s) 1264
BsaAI YACGTR 1 cut(s) 1127
BsaJI CCNNGG 8 cut(s) 748, 749, 1002, 1469, 1746, 1866, 1901, 2573
BsaWI WCCGGW 1 cut(s) 1460
BsaXI ACNNNNNCTCC 2 cut(s) 2221, 2251
Bsc4I CCNNNNNNNGG 3 cut(s) 1074, 1845, 1867
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 3 cut(s) 1960, 2382, 2422
Bse3DI GCAATG 1 cut(s) 771
BseBI CCWGG 2 cut(s) 750, 2174
BseCI ATCGAT 1 cut(s) 1264
BseDI CCNNGG 8 cut(s) 748, 749, 1002, 1469, 1746, 1866, 1901, 2573
BseGI GGATG 4 cut(s) 62, 536, 841, 938
BseLI CCNNNNNNNGG 3 cut(s) 1074, 1845, 1867
BseMI GCAATG 1 cut(s) 771
BseMII CTCAG 4 cut(s) 21, 638, 1683, 2420
BseNI ACTGG 3 cut(s) 1960, 2382, 2422
BseRI GAGGAG 1 cut(s) 2159
BseXI GCAGC 4 cut(s) 374, 1905, 2479, 2514
BsgI GTGCAG 2 cut(s) 2217, 2460
Bsh1236I CGCG 1 cut(s) 993
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 6 cut(s) 5, 206, 1001, 1131, 1468, 2381
BshNI GGYRCC 2 cut(s) 2191, 2284
BshVI ATCGAT 1 cut(s) 1264
BsiEI CGRYCG 1 cut(s) 271
BsiHKAI GWGCWC 2 cut(s) 1011, 2169
BsiHKCI CYCGRG 1 cut(s) 2345
BsiSI CCGG 3 cut(s) 75, 1461, 1748
BslFI GGGAC 4 cut(s) 290, 731, 1729, 2002
BslI CCNNNNNNNGG 3 cut(s) 1074, 1845, 1867
BsmAI GTCTC 4 cut(s) 571, 623, 634, 2121
BsmBI CGTCTC 1 cut(s) 2121
BsmFI GGGAC 4 cut(s) 290, 731, 1729, 2002
BsmI GAATGC 2 cut(s) 45, 1025
BsnI GGCC 6 cut(s) 5, 206, 1001, 1131, 1468, 2381
BsoBI CYCGRG 1 cut(s) 2345
Bsp1286I GDGCHC 3 cut(s) 1011, 1582, 2169
Bsp143I GATC 6 cut(s) 537, 587, 1227, 1489, 2618, 2665
BspACI CCGC 3 cut(s) 148, 267, 1498
BspANI GGCC 6 cut(s) 5, 206, 1001, 1131, 1468, 2381
BspCNI CTCAG 4 cut(s) 20, 637, 1684, 2421
BspDI ATCGAT 1 cut(s) 1264
BspFNI CGCG 1 cut(s) 993
BspLI GGNNCC 5 cut(s) 1579, 1745, 1850, 2193, 2286
BspMAI CTGCAG 1 cut(s) 2532
BspPI GGATC 3 cut(s) 595, 2626, 2673
BspQI GCTCTTC 1 cut(s) 1016
BspT107I GGYRCC 2 cut(s) 2191, 2284
BspTI CTTAAG 1 cut(s) 1789
BsrBI CCGCTC 1 cut(s) 148
BsrDI GCAATG 1 cut(s) 771
BsrFI RCCGGY 1 cut(s) 74
BsrI ACTGG 3 cut(s) 1960, 2382, 2422
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 8 cut(s) 748, 749, 1002, 1469, 1746, 1866, 1901, 2573
BssMI GATC 6 cut(s) 537, 587, 1227, 1489, 2618, 2665
BssSI CACGAG 1 cut(s) 494
BssT1I CCWWGG 4 cut(s) 1002, 1469, 1866, 1901
Bst2BI CACGAG 1 cut(s) 494
Bst2UI CCWGG 2 cut(s) 750, 2174
Bst4CI ACNGT 8 cut(s) 634, 670, 1171, 1253, 1341, 1543, 1743, 1949
Bst6I CTCTTC 3 cut(s) 919, 1016, 2196
BstAFI CTTAAG 1 cut(s) 1789
BstAPI GCANNNNNTGC 1 cut(s) 2240
BstBAI YACGTR 1 cut(s) 1127
BstC8I GCNNGC 7 cut(s) 208, 762, 1433, 1477, 1506, 2383, 2443
BstDEI CTNAG 8 cut(s) 7, 624, 1069, 1196, 1241, 1692, 2097, 2429
BstDSI CCRYGG 1 cut(s) 2573
BstF5I GGATG 4 cut(s) 62, 536, 841, 938
BstFNI CGCG 1 cut(s) 993
BstHHI GCGC 1 cut(s) 2227
BstKTI GATC 6 cut(s) 540, 590, 1230, 1492, 2621, 2668
BstMAI GTCTC 4 cut(s) 571, 623, 634, 2121
BstMBI GATC 6 cut(s) 537, 587, 1227, 1489, 2618, 2665
BstMCI CGRYCG 1 cut(s) 271
BstMWI GCNNNNNNNGC 7 cut(s) 371, 1118, 2231, 2240, 2391, 2467, 2527
BstNI CCWGG 2 cut(s) 750, 2174
BstNSI RCATGY 6 cut(s) 250, 764, 793, 1435, 1479, 2043
BstSCI CCNGG 3 cut(s) 748, 1746, 2172
BstSFI CTRYAG 4 cut(s) 772, 1155, 2502, 2528
BstUI CGCG 1 cut(s) 993
BstV1I GCAGC 4 cut(s) 374, 1905, 2479, 2514
BstV2I GAAGAC 5 cut(s) 602, 699, 936, 1143, 2204
BstX2I RGATCY 1 cut(s) 2618
BstYI RGATCY 1 cut(s) 2618
Bsu15I ATCGAT 1 cut(s) 1264
BsuI GTATCC 1 cut(s) 133
BsuRI GGCC 6 cut(s) 5, 206, 1001, 1131, 1468, 2381
BsuTUI ATCGAT 1 cut(s) 1264
BtgI CCRYGG 1 cut(s) 2573
BtgZI GCGATG 2 cut(s) 225, 1008
BtrI CACGTC 1 cut(s) 841
BtsCI GGATG 4 cut(s) 62, 536, 841, 938
BtsIMutI CAGTG 6 cut(s) 1539, 1953, 2375, 2415, 2443, 2634
Cac8I GCNNGC 7 cut(s) 208, 762, 1433, 1477, 1506, 2383, 2443
CfoI GCGC 1 cut(s) 2227
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 2 cut(s) 1743, 1997
ClaI ATCGAT 1 cut(s) 1264
CseI GACGC 1 cut(s) 317
Csp6I GTAC 9 cut(s) 162, 1167, 1546, 1777, 1945, 1961, 2192, 2285, 2678
CviQI GTAC 9 cut(s) 162, 1167, 1546, 1777, 1945, 1961, 2192, 2285, 2678
DdeI CTNAG 8 cut(s) 7, 624, 1069, 1196, 1241, 1692, 2097, 2429
DpnI GATC 6 cut(s) 539, 589, 1229, 1491, 2620, 2667
DpnII GATC 6 cut(s) 537, 587, 1227, 1489, 2618, 2665
DraI TTTAAA 1 cut(s) 1800
DriI GACNNNNNGTC 1 cut(s) 1995
EaeI YGGCCR 1 cut(s) 2379
Eam1104I CTCTTC 3 cut(s) 919, 1016, 2196
Eam1105I GACNNNNNGTC 1 cut(s) 1995
EarI CTCTTC 3 cut(s) 919, 1016, 2196
Ecl136II GAGCTC 2 cut(s) 1009, 2167
Eco130I CCWWGG 4 cut(s) 1002, 1469, 1866, 1901
Eco24I GRGCYC 3 cut(s) 1011, 1582, 2169
Eco32I GATATC 3 cut(s) 223, 821, 850
Eco47I GGWCC 2 cut(s) 1743, 1997
Eco53kI GAGCTC 2 cut(s) 1009, 2167
Eco57I CTGAAG 5 cut(s) 997, 1371, 1689, 1957, 2431
Eco88I CYCGRG 1 cut(s) 2345
EcoICRI GAGCTC 2 cut(s) 1009, 2167
EcoRI GAATTC 2 cut(s) 233, 719
EcoRII CCWGG 2 cut(s) 748, 2172
EcoRV GATATC 3 cut(s) 223, 821, 850
EcoT14I CCWWGG 4 cut(s) 1002, 1469, 1866, 1901
EcoT22I ATGCAT 2 cut(s) 1488, 2413
EcoT38I GRGCYC 3 cut(s) 1011, 1582, 2169
ErhI CCWWGG 4 cut(s) 1002, 1469, 1866, 1901
Esp3I CGTCTC 1 cut(s) 2121
FalI AAGNNNNNCTT 4 cut(s) 995, 1027, 1217, 1249
FaqI GGGAC 4 cut(s) 290, 731, 1729, 2002
FauI CCCGC 1 cut(s) 1491
FbaI TGATCA 1 cut(s) 537
Fnu4HI GCNGC 4 cut(s) 363, 1919, 2468, 2528
FokI GGATG 4 cut(s) 49, 543, 848, 925
FriOI GRGCYC 3 cut(s) 1011, 1582, 2169
Fsp4HI GCNGC 4 cut(s) 363, 1919, 2468, 2528
FspBI CTAG 7 cut(s) 50, 134, 824, 1730, 2340, 2639, 2746
GlaI GCGC 1 cut(s) 2226
GluI GCNGC 4 cut(s) 363, 1919, 2468, 2528
GsuI CTGGAG 5 cut(s) 128, 272, 1844, 1977, 2156
HaeIII GGCC 6 cut(s) 5, 206, 1001, 1131, 1468, 2381
HapII CCGG 3 cut(s) 75, 1461, 1748
HgaI GACGC 1 cut(s) 317
HhaI GCGC 1 cut(s) 2227
Hin6I GCGC 1 cut(s) 2225
HinP1I GCGC 1 cut(s) 2225
HincII GTYRAC 1 cut(s) 795
HindII GTYRAC 1 cut(s) 795
HindIII AAGCTT 2 cut(s) 706, 2099
HpaII CCGG 3 cut(s) 75, 1461, 1748
HphI GGTGA 2 cut(s) 2237, 2527
Hpy166II GTNNAC 6 cut(s) 164, 439, 795, 970, 1546, 1777
Hpy188I TCNGA 9 cut(s) 430, 1516, 1528, 1693, 1938, 1976, 2053, 2450, 2711
Hpy8I GTNNAC 6 cut(s) 164, 439, 795, 970, 1546, 1777
Hpy99I CGWCG 2 cut(s) 333, 1587
HpyCH4III ACNGT 8 cut(s) 634, 670, 1171, 1253, 1341, 1543, 1743, 1949
HpyCH4IV ACGT 8 cut(s) 179, 192, 840, 1126, 1273, 1368, 2290, 2714
HpyF10VI GCNNNNNNNGC 7 cut(s) 371, 1118, 2231, 2240, 2391, 2467, 2527
HpyF3I CTNAG 8 cut(s) 7, 624, 1069, 1196, 1241, 1692, 2097, 2429
HpySE526I ACGT 8 cut(s) 179, 192, 840, 1126, 1273, 1368, 2290, 2714
HspAI GCGC 1 cut(s) 2225
KpnI GGTACC 2 cut(s) 2195, 2288
Ksp22I TGATCA 1 cut(s) 537
Kzo9I GATC 6 cut(s) 537, 587, 1227, 1489, 2618, 2665
LguI GCTCTTC 1 cut(s) 1016
LmnI GCTCC 8 cut(s) 16, 109, 1006, 1501, 1577, 1854, 2164, 2172
Lsp1109I GCAGC 4 cut(s) 374, 1905, 2479, 2514
LweI GCATC 9 cut(s) 71, 947, 1201, 1473, 1532, 2115, 2212, 2508, 2529
MaeI CTAG 7 cut(s) 50, 134, 824, 1730, 2340, 2639, 2746
MaeII ACGT 8 cut(s) 179, 192, 840, 1126, 1273, 1368, 2290, 2714
MaeIII GTNAC 8 cut(s) 345, 1094, 1199, 1378, 1635, 1663, 1750, 1949
MalI GATC 6 cut(s) 539, 589, 1229, 1491, 2620, 2667
MbiI CCGCTC 1 cut(s) 148
MboI GATC 6 cut(s) 537, 587, 1227, 1489, 2618, 2665
MfeI CAATTG 1 cut(s) 1113
MflI RGATCY 1 cut(s) 2618
MhlI GDGCHC 3 cut(s) 1011, 1582, 2169
MlsI TGGCCA 1 cut(s) 2381
MluNI TGGCCA 1 cut(s) 2381
MlyI GAGTC 5 cut(s) 473, 1323, 2211, 2337, 2651
MmeI TCCRAC 2 cut(s) 38, 2256
Mox20I TGGCCA 1 cut(s) 2381
Mph1103I ATGCAT 2 cut(s) 1488, 2413
MroXI GAANNNNTTC 4 cut(s) 128, 424, 1025, 2211
MscI TGGCCA 1 cut(s) 2381
MslI CAYNNNNRTG 1 cut(s) 1631
Msp20I TGGCCA 1 cut(s) 2381
MspCI CTTAAG 1 cut(s) 1789
MspI CCGG 3 cut(s) 75, 1461, 1748
MspR9I CCNGG 3 cut(s) 750, 1748, 2174
MunI CAATTG 1 cut(s) 1113
Mva1269I GAATGC 2 cut(s) 45, 1025
MvaI CCWGG 2 cut(s) 750, 2174
MvnI CGCG 1 cut(s) 993
MwoI GCNNNNNNNGC 7 cut(s) 371, 1118, 2231, 2240, 2391, 2467, 2527
NciI CCSGG 1 cut(s) 1748
NdeII GATC 6 cut(s) 537, 587, 1227, 1489, 2618, 2665
NlaIV GGNNCC 5 cut(s) 1579, 1745, 1850, 2193, 2286
NmeAIII GCCGAG 1 cut(s) 487
NmuCI GTSAC 2 cut(s) 1635, 1663
NsiI ATGCAT 2 cut(s) 1488, 2413
NspI RCATGY 6 cut(s) 250, 764, 793, 1435, 1479, 2043
PaeI GCATGC 3 cut(s) 764, 1435, 1479
PaeR7I CTCGAG 1 cut(s) 2345
PasI CCCWGGG 1 cut(s) 749
PciI ACATGT 1 cut(s) 789
PciSI GCTCTTC 1 cut(s) 1016
PcsI WCGNNNNNNNCGW 1 cut(s) 1270
PctI GAATGC 2 cut(s) 45, 1025
PdmI GAANNNNTTC 4 cut(s) 128, 424, 1025, 2211
PfeI GAWTC 8 cut(s) 906, 1217, 1517, 1674, 1726, 1880, 2048, 2326
PflMI CCANNNNNTGG 1 cut(s) 1867
PfoI TCCNGGA 1 cut(s) 2172
PkrI GCNGC 4 cut(s) 364, 1920, 2469, 2529
PleI GAGTC 5 cut(s) 472, 1323, 2211, 2337, 2650
PpsI GAGTC 5 cut(s) 472, 1323, 2211, 2337, 2650
Ppu21I YACGTR 1 cut(s) 1127
PscI ACATGT 1 cut(s) 789
PsiI TTATAA 1 cut(s) 2406
Psp124BI GAGCTC 2 cut(s) 1011, 2169
Psp1406I AACGTT 2 cut(s) 1273, 2714
Psp6I CCWGG 2 cut(s) 748, 2172
PspGI CCWGG 2 cut(s) 748, 2172
PspN4I GGNNCC 5 cut(s) 1579, 1745, 1850, 2193, 2286
PspPI GGNCC 2 cut(s) 1743, 1997
PspXI VCTCGAGB 1 cut(s) 2345
PstI CTGCAG 1 cut(s) 2532
PsuI RGATCY 1 cut(s) 2618
RsaI GTAC 9 cut(s) 163, 1168, 1547, 1778, 1946, 1962, 2193, 2286, 2679
RsaNI GTAC 9 cut(s) 162, 1167, 1546, 1777, 1945, 1961, 2192, 2285, 2678
RseI CAYNNNNRTG 1 cut(s) 1631
SacI GAGCTC 2 cut(s) 1011, 2169
SapI GCTCTTC 1 cut(s) 1016
SatI GCNGC 4 cut(s) 363, 1919, 2468, 2528
Sau3AI GATC 6 cut(s) 537, 587, 1227, 1489, 2618, 2665
Sau96I GGNCC 2 cut(s) 1743, 1997
SchI GAGTC 5 cut(s) 473, 1323, 2211, 2337, 2651
ScrFI CCNGG 3 cut(s) 750, 1748, 2174
SduI GDGCHC 3 cut(s) 1011, 1582, 2169
SfaNI GCATC 9 cut(s) 71, 947, 1201, 1473, 1532, 2115, 2212, 2508, 2529
SfcI CTRYAG 4 cut(s) 772, 1155, 2502, 2528
Sfr274I CTCGAG 1 cut(s) 2345
SinI GGWCC 2 cut(s) 1743, 1997
SlaI CTCGAG 1 cut(s) 2345
SmiI ATTTAAAT 1 cut(s) 1800
SmiMI CAYNNNNRTG 1 cut(s) 1631
SmlI CTYRAG 3 cut(s) 113, 1789, 2345
SmoI CTYRAG 3 cut(s) 113, 1789, 2345
SphI GCATGC 3 cut(s) 764, 1435, 1479
SsiI CCGC 3 cut(s) 148, 267, 1498
SspMI CTAG 7 cut(s) 50, 134, 824, 1730, 2340, 2639, 2746
SstI GAGCTC 2 cut(s) 1011, 2169
StyD4I CCNGG 3 cut(s) 748, 1746, 2172
StyI CCWWGG 4 cut(s) 1002, 1469, 1866, 1901
SwaI ATTTAAAT 1 cut(s) 1800
TaaI ACNGT 8 cut(s) 634, 670, 1171, 1253, 1341, 1543, 1743, 1949
TaiI ACGT 8 cut(s) 182, 195, 843, 1129, 1276, 1371, 2293, 2717
TaqI TCGA 8 cut(s) 271, 331, 904, 922, 1264, 2215, 2220, 2346
TatI WGTACW 4 cut(s) 161, 1166, 1776, 1960
TfiI GAWTC 8 cut(s) 906, 1217, 1517, 1674, 1726, 1880, 2048, 2326
TscAI CASTG 6 cut(s) 1546, 1960, 2382, 2422, 2443, 2634
TseFI GTSAC 2 cut(s) 1635, 1663
TseI GCWGC 4 cut(s) 362, 1918, 2467, 2527
Tsp45I GTSAC 2 cut(s) 1635, 1663
TspGWI ACGGA 1 cut(s) 1197
TspRI CASTG 6 cut(s) 1546, 1960, 2382, 2422, 2443, 2634
Van91I CCANNNNNTGG 1 cut(s) 1867
Vha464I CTTAAG 1 cut(s) 1789
VpaK11BI GGWCC 2 cut(s) 1743, 1997
XapI RAATTY 4 cut(s) 233, 719, 754, 1801
XbaI TCTAGA 4 cut(s) 49, 1729, 2339, 2638
XceI RCATGY 6 cut(s) 250, 764, 793, 1435, 1479, 2043
XcmI CCANNNNNNNNNTGG 1 cut(s) 315
XhoI CTCGAG 1 cut(s) 2345
XmnI GAANNNNTTC 4 cut(s) 128, 424, 1025, 2211
XspI CTAG 7 cut(s) 50, 134, 824, 1730, 2340, 2639, 2746
Zsp2I ATGCAT 2 cut(s) 1488, 2413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.