Rroxscaffold_7G00191240

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000110
Physical Location & Seq
Reverse (-)
31749225 .. 31753689
4465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00191240.1

Sequence Viewer

Length: 1623 bp
ATGTCTTCAAAGAAATCATCTGCTGAGTGGAGAGAAGTGTGCAACAACTTAAATTGGCATCTTACAAACGATCACTTTCTAGAACCGGTGAAGAGCATCTTGTTGCTTAGTTACAATGATTTGCCATACCGTTTGAAGCATTGTTTCTTATATTGTTCCCTTTTCCCAGAAGATCATTTGATTGGAAGATACAGGCTTGTTAGATTGTGGATAGCTGAAGGGTTTATTGAACATGTCAATGGGGTCACACTAGAAGACCTTGCAGAGAGGTATCTTATAGAGCTAACTTTTCGCAGCATGTTGCAATTTGTAAAGAGGGATTCTCAATCTAGGATCCCAGAACAGTGTCTGATGCATGATCTGATGCGGGAGATTGCTCTCTCAACTTCAGAAAGAGAAAAGTTCGGCGTTGTATTTGATAGGAGAGGAGTAATGGAAGAAACTAGAGTTCGCCGTTTGGCGATTCATACAACTGAAGGAGAAATTGAATTGTGCACAGGTATGTTGCAGCTTCGTACATTTCTTGTGTTTCCTTCTGGCTTACGTTCTCCATTGTTATCAAATACATGGTCTTCTGGGTTCTACTTGTTGAGAGTTCTAGATCTGGAAAATGTACCAATTGATAAGCTTCCAGACAATCTAGTATACTTATTCAATTTAAGATATTTGAATCTTAGAGGAACTCGGATTAAAAGGCTTCCAAAAGACATTGGAAGGCTCCACAACCTTCAAACCTTGGACATCAGCGACACAAACATAAAGATACTTCCTAGTAGAAGTTCAGAGTTGATAAACCTGCGCCATCTGTATATGGGACATTTCAATGATACTGGATTTGAGACCTTCATATGTGCTAGTGGGATTAGAGCATCATCACATATTTCTATATTCAAGAAGTTGCAAGATTTATTTTTTGTTCGGTCAGATAAAAACATGATCAGACTTCTGAGAAAAATGACCCAACTTAGGATGCTTGGCATTACAAATGTAAAAGAAAGTGCTGAAATAGAGCTCTGTGCCTCAATTCAGAAGATGAAGATCCTGGTCTGGTTGAATTTATCAGTAAGTGATAGTTCGGAGGGATTTCTTCGAATTGACGCACTTACTTTACCTCCTCCATATCTTGAGTCCCTTTTGTTACGTGGTAAACTGGAAAGCATACCATGTTGGTTTTGTTCATTACAGTGCCTCACACATATAGAGCTGCTTTGGTGTAGACTTGAAGAAGATTTACTGCCTCACATTGAGGCGCTTCCCTGTCTGGAACGTATTCTCCTTGATAATGCTTATGTTGGGAAAGAGATGAGTTTCAGTAGAGGCTTTGCAAAGCTTACACATTTGGAGTTGTTCAATTTCCGAGTATTGAATAAGGTAAGTGTAGAAAAAGGGGTGATGCCAAATCTCCAATACCTGGGACTTCGAAGCTGCAAGAAATTGAAGACATTGCCGCATGGTCTTGAATACCTTACTCATCTCGAAAAATTGTATTTGGAGTCTGTTACAAGGAACCTTATAGAGTCCATACGAGAAGGCGGTGTGGATCGTCCAAAGGTACAACACATCCGCATAATTGACCATCATTACAAAACATCTTCTGGGTTGTCTTACGAGAGCTTGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

540

Amino Acids

62.81

Weight (kDa)

8.66

Isoelectric Point (pI)

58.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 54 - 126 1.6e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 170 - 497 1.3e-40 Leucine-rich repeat region
LRR_8 PF13855 219 - 273 1.4e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 804
AccB7I CCANNNNNTGG 1 cut(s) 1411
AccI GTMKAC 2 cut(s) 645, 1216
AciI CCGC 4 cut(s) 367, 1448, 1533, 1564
AclWI GGATC 4 cut(s) 328, 341, 1033, 1548
AcsI RAATTY 1 cut(s) 1054
AcuI CTGAAG 3 cut(s) 237, 372, 495
AfaI GTAC 3 cut(s) 517, 615, 1554
AfiI CCNNNNNNNGG 2 cut(s) 966, 1411
AflIII ACRYGT 1 cut(s) 232
AgeI ACCGGT 1 cut(s) 85
AjnI CCWGG 2 cut(s) 1041, 1410
AloI GAACNNNNNNTCC 2 cut(s) 1257, 1289
AluBI AGCT 9 cut(s) 215, 283, 511, 628, 1012, 1204, 1330, 1425, 1614
AluI AGCT 9 cut(s) 215, 283, 511, 628, 1012, 1204, 1330, 1425, 1614
Alw21I GWGCWC 2 cut(s) 497, 1014
Alw26I GTCTC 1 cut(s) 833
Alw44I GTGCAC 1 cut(s) 493
AlwI GGATC 4 cut(s) 328, 341, 1033, 1548
AlwNI CAGNNNCTG 1 cut(s) 349
ApaLI GTGCAC 1 cut(s) 493
ApeKI GCWGC 4 cut(s) 294, 508, 1204, 1425
ApoI RAATTY 1 cut(s) 1054
ArsI GACNNNNNNTTYG 2 cut(s) 554, 586
AsiGI ACCGGT 1 cut(s) 85
Asp700I GAANNNNTTC 1 cut(s) 1269
AspLEI GCGC 2 cut(s) 801, 1252
AsuHPI GGTGA 2 cut(s) 100, 1402
AsuII TTCGAA 2 cut(s) 1090, 1420
BaeGI GKGCMC 1 cut(s) 497
BamHI GGATCC 1 cut(s) 333
BanII GRGCYC 1 cut(s) 1014
BarI GAAGNNNNNNTAC 2 cut(s) 1215, 1247
BbsI GAAGAC 3 cut(s) 261, 564, 1445
Bbv12I GWGCWC 2 cut(s) 497, 1014
BbvI GCAGC 4 cut(s) 306, 520, 1191, 1412
BccI CCATC 2 cut(s) 810, 1584
BceAI ACGGC 1 cut(s) 438
BciT130I CCWGG 2 cut(s) 1043, 1412
BclI TGATCA 1 cut(s) 936
BcoDI GTCTC 1 cut(s) 833
BfaI CTAG 9 cut(s) 80, 251, 330, 444, 599, 641, 771, 855, 1621
BfoI RGCGCY 1 cut(s) 1253
BfuAI ACCTGC 1 cut(s) 804
BglII AGATCT 1 cut(s) 601
BisI GCNGC 5 cut(s) 295, 509, 1205, 1426, 1448
BlsI GCNGC 5 cut(s) 296, 510, 1206, 1427, 1449
Bme1390I CCNGG 2 cut(s) 1043, 1412
BmiI GGNNCC 3 cut(s) 335, 719, 1508
BmrFI CCNGG 2 cut(s) 1043, 1412
BmsI GCATC 7 cut(s) 67, 105, 342, 354, 878, 960, 1383
BpiI GAAGAC 3 cut(s) 261, 564, 1445
BplI GAGNNNNNCTC 2 cut(s) 307, 339
Bpu14I TTCGAA 2 cut(s) 1090, 1420
BpuEI CTTGAG 1 cut(s) 1145
BsaAI YACGTR 1 cut(s) 1142
BsaBI GATNNNNATC 1 cut(s) 1037
BsaI GGTCTC 1 cut(s) 833
BsaJI CCNNGG 2 cut(s) 735, 1411
BsaWI WCCGGW 1 cut(s) 85
BsaXI ACNNNNNCTCC 4 cut(s) 1096, 1126, 1257, 1287
Bsc4I CCNNNNNNNGG 2 cut(s) 966, 1411
Bse118I RCCGGY 1 cut(s) 85
Bse1I ACTGG 2 cut(s) 835, 1155
Bse3DI GCAATG 1 cut(s) 1442
Bse8I GATNNNNATC 1 cut(s) 1037
BseBI CCWGG 2 cut(s) 1043, 1412
BseDI CCNNGG 2 cut(s) 735, 1411
BseGI GGATG 2 cut(s) 975, 1560
BseJI GATNNNNATC 1 cut(s) 1037
BseLI CCNNNNNNNGG 2 cut(s) 966, 1411
BseMI GCAATG 1 cut(s) 1442
BseMII CTCAG 2 cut(s) 15, 938
BseNI ACTGG 2 cut(s) 835, 1155
BseRI GAGGAG 2 cut(s) 441, 1104
BseSI GKGCMC 1 cut(s) 497
BseXI GCAGC 4 cut(s) 306, 520, 1191, 1412
BshTI ACCGGT 1 cut(s) 85
BsiHKAI GWGCWC 2 cut(s) 497, 1014
BsiSI CCGG 1 cut(s) 86
BslFI GGGAC 3 cut(s) 828, 1114, 1428
BslI CCNNNNNNNGG 2 cut(s) 966, 1411
BsmAI GTCTC 1 cut(s) 833
BsmFI GGGAC 3 cut(s) 828, 1114, 1428
Bso31I GGTCTC 1 cut(s) 833
Bsp119I TTCGAA 2 cut(s) 1090, 1420
Bsp1286I GDGCHC 2 cut(s) 497, 1014
Bsp143I GATC 8 cut(s) 70, 172, 333, 358, 601, 936, 1038, 1540
BspACI CCGC 4 cut(s) 367, 1448, 1533, 1564
BspCNI CTCAG 2 cut(s) 16, 939
BspLI GGNNCC 3 cut(s) 335, 719, 1508
BspMI ACCTGC 1 cut(s) 804
BspPI GGATC 4 cut(s) 328, 341, 1033, 1548
BspQI GCTCTTC 1 cut(s) 86
BspT104I TTCGAA 2 cut(s) 1090, 1420
BspTNI GGTCTC 1 cut(s) 833
BsrDI GCAATG 1 cut(s) 1442
BsrFI RCCGGY 1 cut(s) 85
BsrI ACTGG 2 cut(s) 835, 1155
BssAI RCCGGY 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 735, 1411
BssMI GATC 8 cut(s) 70, 172, 333, 358, 601, 936, 1038, 1540
BssNAI GTATAC 1 cut(s) 646
BssT1I CCWWGG 1 cut(s) 735
Bst1107I GTATAC 1 cut(s) 646
Bst2UI CCWGG 2 cut(s) 1043, 1412
Bst4CI ACNGT 3 cut(s) 131, 345, 1185
Bst6I CTCTTC 1 cut(s) 86
BstBAI YACGTR 1 cut(s) 1142
BstBI TTCGAA 2 cut(s) 1090, 1420
BstDEI CTNAG 5 cut(s) 24, 107, 674, 947, 965
BstF5I GGATG 2 cut(s) 975, 1560
BstH2I RGCGCY 1 cut(s) 1253
BstHHI GCGC 2 cut(s) 801, 1252
BstKTI GATC 8 cut(s) 73, 175, 336, 361, 604, 939, 1041, 1543
BstMAI GTCTC 1 cut(s) 833
BstMBI GATC 8 cut(s) 70, 172, 333, 358, 601, 936, 1038, 1540
BstNI CCWGG 2 cut(s) 1043, 1412
BstNSI RCATGY 2 cut(s) 236, 301
BstSCI CCNGG 2 cut(s) 1041, 1410
BstSLI GKGCMC 1 cut(s) 497
BstV1I GCAGC 4 cut(s) 306, 520, 1191, 1412
BstV2I GAAGAC 3 cut(s) 261, 564, 1445
BstX2I RGATCY 3 cut(s) 333, 601, 1038
BstYI RGATCY 3 cut(s) 333, 601, 1038
BstZ17I GTATAC 1 cut(s) 646
BtsCI GGATG 2 cut(s) 975, 1560
BtsIMutI CAGTG 2 cut(s) 350, 1190
BveI ACCTGC 1 cut(s) 804
CaiI CAGNNNCTG 1 cut(s) 349
CfoI GCGC 2 cut(s) 801, 1252
Cfr10I RCCGGY 1 cut(s) 85
CseI GACGC 1 cut(s) 1106
Csp6I GTAC 3 cut(s) 516, 614, 1553
CspAI ACCGGT 1 cut(s) 85
CviAII CATG 7 cut(s) 233, 298, 356, 567, 934, 1164, 1451
CviQI GTAC 3 cut(s) 516, 614, 1553
DdeI CTNAG 5 cut(s) 24, 107, 674, 947, 965
DpnI GATC 8 cut(s) 72, 174, 335, 360, 603, 938, 1040, 1542
DpnII GATC 8 cut(s) 70, 172, 333, 358, 601, 936, 1038, 1540
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
Ecl136II GAGCTC 1 cut(s) 1012
Eco130I CCWWGG 1 cut(s) 735
Eco24I GRGCYC 1 cut(s) 1014
Eco31I GGTCTC 1 cut(s) 833
Eco53kI GAGCTC 1 cut(s) 1012
Eco57I CTGAAG 3 cut(s) 237, 372, 495
EcoICRI GAGCTC 1 cut(s) 1012
EcoRII CCWGG 2 cut(s) 1041, 1410
EcoT14I CCWWGG 1 cut(s) 735
EcoT22I ATGCAT 1 cut(s) 357
EcoT38I GRGCYC 1 cut(s) 1014
ErhI CCWWGG 1 cut(s) 735
FaeI CATG 7 cut(s) 236, 301, 359, 570, 937, 1167, 1454
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FaqI GGGAC 3 cut(s) 828, 1114, 1428
FatI CATG 7 cut(s) 232, 297, 355, 566, 933, 1163, 1450
FauI CCCGC 1 cut(s) 360
FauNDI CATATG 1 cut(s) 848
FbaI TGATCA 1 cut(s) 936
FblI GTMKAC 2 cut(s) 645, 1216
Fnu4HI GCNGC 5 cut(s) 295, 509, 1205, 1426, 1448
FokI GGATG 2 cut(s) 982, 1547
FriOI GRGCYC 1 cut(s) 1014
Fsp4HI GCNGC 5 cut(s) 295, 509, 1205, 1426, 1448
FspBI CTAG 9 cut(s) 80, 251, 330, 444, 599, 641, 771, 855, 1621
GlaI GCGC 2 cut(s) 800, 1251
GluI GCNGC 5 cut(s) 295, 509, 1205, 1426, 1448
HaeII RGCGCY 1 cut(s) 1253
HapII CCGG 1 cut(s) 86
HgaI GACGC 1 cut(s) 1106
HhaI GCGC 2 cut(s) 801, 1252
Hin1II CATG 7 cut(s) 236, 301, 359, 570, 937, 1167, 1454
Hin6I GCGC 2 cut(s) 799, 1250
HinP1I GCGC 2 cut(s) 799, 1250
HindIII AAGCTT 2 cut(s) 626, 1328
HinfI GANTC 6 cut(s) 320, 463, 670, 1127, 1493, 1517
HpaII CCGG 1 cut(s) 86
HphI GGTGA 2 cut(s) 100, 1402
Hpy166II GTNNAC 4 cut(s) 495, 646, 1148, 1217
Hpy188III TCNNGA 9 cut(s) 80, 599, 605, 632, 892, 1124, 1262, 1457, 1475
Hpy8I GTNNAC 4 cut(s) 495, 646, 1148, 1217
HpyAV CCTTC 7 cut(s) 212, 470, 543, 708, 737, 853, 1523
HpyCH4III ACNGT 3 cut(s) 131, 345, 1185
HpyCH4IV ACGT 3 cut(s) 544, 1141, 1267
HpyCH4V TGCA 9 cut(s) 42, 263, 304, 355, 495, 508, 901, 1325, 1428
HpyF3I CTNAG 5 cut(s) 24, 107, 674, 947, 965
HpySE526I ACGT 3 cut(s) 544, 1141, 1267
Hsp92II CATG 7 cut(s) 236, 301, 359, 570, 937, 1167, 1454
HspAI GCGC 2 cut(s) 799, 1250
Ksp22I TGATCA 1 cut(s) 936
Kzo9I GATC 8 cut(s) 70, 172, 333, 358, 601, 936, 1038, 1540
LguI GCTCTTC 1 cut(s) 86
LmnI GCTCC 1 cut(s) 723
Lsp1109I GCAGC 4 cut(s) 306, 520, 1191, 1412
LweI GCATC 7 cut(s) 67, 105, 342, 354, 878, 960, 1383
MaeI CTAG 9 cut(s) 80, 251, 330, 444, 599, 641, 771, 855, 1621
MaeII ACGT 3 cut(s) 544, 1141, 1267
MaeIII GTNAC 4 cut(s) 110, 244, 1137, 1498
MalI GATC 8 cut(s) 72, 174, 335, 360, 603, 938, 1040, 1542
MboI GATC 8 cut(s) 70, 172, 333, 358, 601, 936, 1038, 1540
MfeI CAATTG 1 cut(s) 618
MflI RGATCY 3 cut(s) 333, 601, 1038
MhlI GDGCHC 2 cut(s) 497, 1014
MlyI GAGTC 3 cut(s) 1136, 1502, 1526
Mph1103I ATGCAT 1 cut(s) 357
MroXI GAANNNNTTC 1 cut(s) 1269
MseI TTAA 3 cut(s) 50, 659, 690
MslI CAYNNNNRTG 4 cut(s) 237, 500, 822, 1183
MspI CCGG 1 cut(s) 86
MspR9I CCNGG 2 cut(s) 1043, 1412
MunI CAATTG 1 cut(s) 618
MvaI CCWGG 2 cut(s) 1043, 1412
NdeI CATATG 1 cut(s) 848
NdeII GATC 8 cut(s) 70, 172, 333, 358, 601, 936, 1038, 1540
NlaIII CATG 7 cut(s) 236, 301, 359, 570, 937, 1167, 1454
NlaIV GGNNCC 3 cut(s) 335, 719, 1508
NmuCI GTSAC 1 cut(s) 244
NsiI ATGCAT 1 cut(s) 357
NspI RCATGY 2 cut(s) 236, 301
NspV TTCGAA 2 cut(s) 1090, 1420
PciI ACATGT 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 86
PdmI GAANNNNTTC 1 cut(s) 1269
PfeI GAWTC 3 cut(s) 320, 463, 670
PflMI CCANNNNNTGG 1 cut(s) 1411
PinAI ACCGGT 1 cut(s) 85
PkrI GCNGC 5 cut(s) 296, 510, 1206, 1427, 1449
PleI GAGTC 3 cut(s) 1135, 1501, 1525
PpsI GAGTC 3 cut(s) 1135, 1501, 1525
Ppu21I YACGTR 1 cut(s) 1142
PscI ACATGT 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 1014
Psp6I CCWGG 2 cut(s) 1041, 1410
PspGI CCWGG 2 cut(s) 1041, 1410
PspN4I GGNNCC 3 cut(s) 335, 719, 1508
PstNI CAGNNNCTG 1 cut(s) 349
PsuI RGATCY 3 cut(s) 333, 601, 1038
RsaI GTAC 3 cut(s) 517, 615, 1554
RsaNI GTAC 3 cut(s) 516, 614, 1553
RseI CAYNNNNRTG 4 cut(s) 237, 500, 822, 1183
SacI GAGCTC 1 cut(s) 1014
SapI GCTCTTC 1 cut(s) 86
SaqAI TTAA 3 cut(s) 50, 659, 690
SatI GCNGC 5 cut(s) 295, 509, 1205, 1426, 1448
Sau3AI GATC 8 cut(s) 70, 172, 333, 358, 601, 936, 1038, 1540
SchI GAGTC 3 cut(s) 1136, 1502, 1526
ScrFI CCNGG 2 cut(s) 1043, 1412
SduI GDGCHC 2 cut(s) 497, 1014
SfaNI GCATC 7 cut(s) 67, 105, 342, 354, 878, 960, 1383
SfuI TTCGAA 2 cut(s) 1090, 1420
SmiMI CAYNNNNRTG 4 cut(s) 237, 500, 822, 1183
SmlI CTYRAG 1 cut(s) 1124
SmoI CTYRAG 1 cut(s) 1124
SsiI CCGC 4 cut(s) 367, 1448, 1533, 1564
SspMI CTAG 9 cut(s) 80, 251, 330, 444, 599, 641, 771, 855, 1621
SstI GAGCTC 1 cut(s) 1014
StyD4I CCNGG 2 cut(s) 1041, 1410
StyI CCWWGG 1 cut(s) 735
TaaI ACNGT 3 cut(s) 131, 345, 1185
TaiI ACGT 3 cut(s) 547, 1144, 1270
TaqI TCGA 3 cut(s) 1090, 1420, 1476
TaqII GACCGA 1 cut(s) 909
TauI GCSGC 1 cut(s) 1450
TfiI GAWTC 3 cut(s) 320, 463, 670
Tru1I TTAA 3 cut(s) 50, 659, 690
Tru9I TTAA 3 cut(s) 50, 659, 690
TscAI CASTG 2 cut(s) 350, 1190
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 4 cut(s) 294, 508, 1204, 1425
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 4 cut(s) 455, 835, 1049, 1167
TspRI CASTG 2 cut(s) 350, 1190
Van91I CCANNNNNTGG 1 cut(s) 1411
VneI GTGCAC 1 cut(s) 493
XapI RAATTY 1 cut(s) 1054
XbaI TCTAGA 2 cut(s) 79, 598
XceI RCATGY 2 cut(s) 236, 301
XmiI GTMKAC 2 cut(s) 645, 1216
XmnI GAANNNNTTC 1 cut(s) 1269
XspI CTAG 9 cut(s) 80, 251, 330, 444, 599, 641, 771, 855, 1621
Zsp2I ATGCAT 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.