MD06G1153100.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000110
Physical Location & Seq
Forward (+)
29624532 .. 29630691
6160 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1153100.v1.1.491

Sequence Viewer

Length: 4092 bp
ATGGAGTCAGCTGCATCACTATTGATTGGGAAAATTGCGGCCATTCTTGAGAACGAAGCATCTTCCATAGCGGCAGTTCGAGATGAAGTTGATGAGCTTAAGCTGGAGCTCATAAGCATGAAATCTTTCTTAATAGATGCTGAAAGCAAGGAACCACAAACAGAAGGAGAGAAAACGTGGGTTACAAGCGTCAGAGATTTGACCTGCGATGCTGAAAATGTCATTGATGAGTTCCTGTATCACATATATGACAAGCAAAGTGCGACTCCATTTGAAAAATTGCTCCACAGAACCATTTACTTTCCAAAGAATCTTTGGCATCGGCATCGAATAGCCAAAAAATTACAGAAAATCACAAAAAAGATCAAAGCCATTCCCGAGAGGAATGACAGATATCGTGTCTCTACAATAGAAGGAACAAGTTCGGATAGTGTTCCCAGATGGGTGAAGAACAAAGCCAAGTCTTCTCTTTATATTATGGAAGACGAACTAATCGGGATTGAAGACAAGAAGCAAAGGTTAATGGGGTTATTGATGAATGGAGAGGAAAATGAAATGGTTGTGTCTGTGGTCGGGATGGGAGGATCAGGCAAGACAACTCTTGTTGCCAATACCTTCAACAACGAAAATGTAAAGCGGCATTTTGACTGTTGTGCATGGATCACTGTTTCTCAAACTTTTGTGATCGAAGACTTATTCAAAAATCTGATCAAGCAATTCCACCAAGGAAGAAAGGAAGAGGTGACTGCACAATTGGATTCCATGAGTTACAAAGAATTGTTAGAGATGTTGTCGACATACTTGAAGTCTAAAACGTACCTTGTTGTATTGGATGATGTGTGGGACATTAAACTTTGGCAAGAAATAAGGATACCACTTCTTAATAGACATCATGGAAGTCGAATCATGCTTACAACTCGAAAGAAAGACATAGCATTCTATTCTTTTGAAGTTGAAAGTCGTCCTTTTGAAATTGAACCCTTGGGAAACAATGAAGCTTGGGAGCTCTTCAGCAAGAAAGCATTCTCAACTTACGATAATAAATCTTGTCCACCAGAGCTTGAATCCTTAGCATGGAAACTTGTGGATAAGTGTGAAGGCCTACCTCTGCCAGTGGTAACTTTAGGTGGTCAAATGTCTTCCAAGAGGTCATCATCGGAATGGAGAAGCGTATATAGCAGCTTAAATTGGCACTTGACTAACCATCCTATGCTGGAACCAATGAGCAACATCTTGTTGCTTAGTTTCAACAATTTGCCCAACCGGTTGAAGCCATGTTTCCTATATTGTGCCCTTTTCCCAGAAGATTATCTCATCAAAAGAAAAAGGTTGATCAGGTTGTGGATAGCTGAAGGGTTTGTTGAACCAATTGATGGGGTCACACCAGAAGAAGTTGCAGAGGGCTATCTTGTGGAACTTATTGTTTGTAGCATGCTACAGGTTGAAAAGAATGAAGCTGGAGGACTAAAAGCATGTAAGATGCATGATCTATTGCGTGAGCTTGCTTTGTCGACATCAAAAAAGGAAAAGTTTGGTGCTACGTATGTTGGCAGAGAAATAGTAGATAACGGTGAAATCCGCCGACTGTCAATTCAAACAACTGAAAGAGAAATTAATTCTTGCCCAGGTATGTCAAAGCTTCGCTGCTTTTTTGTCTTTGGCGCTGGCATATTTATGTTCAAGAAATTGCCTTCTGGATTCAAGTCGTTGAGAGTTCTATATTTGGAGAATGCTCCAATTGAAAGATTTCCAGATGAACTAGTGTACTTGTTCAACTTAAGATATTTAAATTTGAAGGGAACTTTAATTGAGGAGCTTCCAGAATCCATCGGACGGCTGCGCAACCTTCAAACCTTGAACATCCGTGACAGTAAGATAAAGGCACTTCCAAAAGCAATCTCCAAGTTCGTAAACCTACGCCATCTAATCATGTATTGTTACGCTGTTGATTTGATGGCCTTTAGATTTCTCAATGGGACAAAAGCAGCATCCGATCTAAGTACATTACAGAAACTGCAAGTTTTGCAATCTGTTGAATCAGAAGCAAAGATTATTAAGGGCATCAGGAATATGACCCAACTTACAAGCCTTGGTATTACAAATGTGAAAGCATGTGACGAGATGGACCTCTGTGACTCCCTTCAAAAGTTAGAGCTCCTTCACTCTTTGTTTTTAATGGCTAGTGATGAAGAGGGGTTTCTTCAAGTTAATGCACTACATTCGCCTCCTCCAGACCTTCAAAAGGTTATTTTGGCCGGAAAACTAGAAAAGGTTCCTCTTTGGTTTGGTTCACTCCATAACCTGACACGTATGTGTCTACATTGGTCTAGACTTGAAGAAGATCTGCTGCCTCACATTCAAGCATTGCCGAATCTTGAAAGGCTTACCCTATCTAATGCATATGTTGGCGAAAATCTGTGTTTCTCCAGAGGCTTCATAAAGCTTAAGCATTTGCTGTTGGGCAGCTTCTCGGTATTGAATAGTATAGCTATAGAGAAAGGGGCGATGCCAAATCTCCATGTCTTAGACATTGGGAACTGCATGGGGTTAAAGGCATTGCCGCAGGGCATTGAGTTCCTTGCTAACGTAGAACGCCTGGATCTGTATTCTGTTCCAATGCAACTTATAGAGTCCGTAAGAGAGGCATGGATCATCCAAAGGTACAACATATTCCTGAAATCCACCTGTATTATGAGAGAGAAAATATGTGGTGTCATGAAAGCTTTTCTAGTATTCACTCAAGAATCAAGCCTCAGAAGCACTTGTGAGACTGCAAAAGCAATCCCGACCGGGTCGGGGCGGATTCGAGGCCCAATGAGCAGCATCTTGTTGCTTAGTTTCAACAATTTGCCCAACCGGTTGAAGCCATGTTTCCTATATTGTGCCCTTTTCCCAGAAGATTATCTCATCAAAAGAAAAAGGTTGATCAGGTTGTGGATAGCTGAAGGGTTTGTTGAACCAATTGATGGGATCACACCAAAAGAAGTTGCAGAGGGTTATCTTGTGGAACTTATTGTTCGTAGCATGCTACAAGTTGAAAAGCATGGAGCTGGAGGACTAAGAGCATGTAAGATGCATGATCTTGTGCGTGAGCTTGCTTTGTCGATATCAAAAAAGGAAAAGTTCGGTGCTACGTGTGTTTCCAGAGAAATAGTAGATAAAGCTGAAATCCGCCGATTGTCAATTCAAACAACTGAAAGAGAAATTAATTCTTGCACGGGTATGTCAGAGCTTCGCTCCTTTCTTGTCTTTGGCGCGTTAGAGAAATTGCCTTCTGGATTCGAGTTGTTGAGAGTTCTAGATTTGCAGGATGCTCCAATTGATAGATTTCTAGATGAACTAGTGTACTTGTTCAACTTAAGATATTTAAATTTGAAGAGAACTTTGATTGACGAGCTTCCAGAATCCATCGGACGACTTCGCAACCTTCAAACCTTGATCATCAGTAACAGTAAGATAAAGGCACTTCCAAAAGCAATCTCCAAGTTAGTAAACCTACGCCATCTAATCATGTTTCGTTACACTGGTGACCATTATGGCTTTATATATCTCAATGGGACAAAAGCAGCATCCGATCTAAGTAAATTACAGAAACCGCAAGTTTTGCATTCCGTTGAATCAGAAGGAAAGATAATTAAAGGCATCAGGAATATGACCCAACTTACAAGCCTCGGTATTACAAATGTGAAAGCAAGTGACGAGATGGACCTCTGTGACTCCCTTCAAAAGTTAGAGCTCCTTCACAATTTGTTTTTAATGGCTGGTGATGAAGAGGAGTTTCTTCAAGTTAATTTGACTCCTCCAGACCTTCAAATTGTTAATGTGACTGGAAAACTAGAAAAGGTACCTCTTTGGTTAGGTTCACTCCATAACCTGACACGTATTTATCTACGTTGGTCTAGACTTGAAGAAGACGTGCTACCTCACATTCAAGCATTGCCGAATCTTGAAAGGCTTTCGCTAATTAATGCATATGTTGGCGAAAAATTGTGTTTCTCCAGAGGCTTCATAAAGCTTAAGCATTTGAGGTTGTTCAAGTTCCCCTTATTGGATAGTATAGCTATAGAGAAAGGGGCGATGCCAAATCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1364

Amino Acids

155.39

Weight (kDa)

8.52

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 87 6.1e-20 Rx N-terminal domain
NB-ARC PF00931 168 - 344 3.3e-46 NB-ARC domain
WHD_DRP PF23559 432 - 502 2.7e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 546 - 845 4.9e-41 Leucine-rich repeat region
WHD_DRP PF23559 963 - 1033 8.1e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 1078 - 1363 6.6e-39 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1841
Acc36I ACCTGC 1 cut(s) 212
Acc65I GGTACC 1 cut(s) 3844
AccB1I GGYRCC 1 cut(s) 3844
AccB7I CCANNNNNTGG 2 cut(s) 1373, 2966
AccI GTMKAC 3 cut(s) 794, 1511, 2317
AccII CGCG 1 cut(s) 3257
AciI CCGC 8 cut(s) 38, 71, 637, 1579, 2561, 2800, 3172, 3596
AclWI GGATC 5 cut(s) 592, 668, 2607, 2657, 2978
AcoI YGGCCR 2 cut(s) 39, 2253
AcsI RAATTY 2 cut(s) 1789, 3370
AcuI CTGAAG 3 cut(s) 994, 1371, 2964
AfaI GTAC 6 cut(s) 818, 1766, 2002, 2663, 3347, 3846
AfiI CCNNNNNNNGG 7 cut(s) 1074, 1373, 1833, 2242, 2796, 2966, 4048
AflII CTTAAG 5 cut(s) 98, 1777, 2444, 3358, 4016
AflIII ACRYGT 3 cut(s) 2306, 3134, 3878
AgeI ACCGGT 2 cut(s) 1263, 2856
AhlI ACTAGT 2 cut(s) 1759, 3340
AjiI CACGTC 1 cut(s) 3916
AjnI CCWGG 2 cut(s) 1624, 2595
AleI CACNNNNGTG 1 cut(s) 2311
Alw21I GWGCWC 4 cut(s) 111, 1008, 2157, 3738
Alw26I GTCTC 2 cut(s) 406, 2762
AlwI GGATC 5 cut(s) 592, 668, 2607, 2657, 2978
AlwNI CAGNNNCTG 1 cut(s) 2014
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 5 cut(s) 39, 1099, 1956, 2253, 2809
ApeKI GCWGC 9 cut(s) 11, 1179, 1644, 1837, 1985, 2347, 2463, 2820, 3566
ApoI RAATTY 2 cut(s) 1789, 3370
AseI ATTAAT 3 cut(s) 1614, 3207, 3966
AsiGI ACCGGT 2 cut(s) 1263, 2856
Asp700I GAANNNNTTC 5 cut(s) 125, 421, 1022, 1746, 2271
Asp718I GGTACC 1 cut(s) 3844
AspLEI GCGC 3 cut(s) 1664, 1842, 3257
AspS9I GGNCC 3 cut(s) 2125, 2810, 3706
AsuC2I CCSGG 1 cut(s) 2791
AsuHPI GGTGA 5 cut(s) 457, 754, 1583, 3539, 3776
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 2 cut(s) 2125, 3706
BaeGI GKGCMC 2 cut(s) 1294, 2887
BanI GGYRCC 1 cut(s) 3844
BanII GRGCYC 4 cut(s) 111, 1008, 2157, 3738
BarI GAAGNNNNNNTAC 2 cut(s) 3903, 3935
BbsI GAAGAC 6 cut(s) 456, 489, 510, 696, 1131, 3918
Bbv12I GWGCWC 4 cut(s) 111, 1008, 2157, 3738
BbvI GCAGC 8 cut(s) 1191, 1631, 1824, 1997, 2334, 2475, 2832, 3578
BceAI ACGGC 1 cut(s) 1850
BcgI CGANNNNNNTGC 4 cut(s) 308, 342, 2202, 2236
BciT130I CCWGG 2 cut(s) 1626, 2597
BciVI GTATCC 1 cut(s) 864
BclI TGATCA 4 cut(s) 708, 1332, 2925, 3438
BcnI CCSGG 1 cut(s) 2791
BcoDI GTCTC 2 cut(s) 406, 2762
BcuI ACTAGT 2 cut(s) 1759, 3340
BfmI CTRYAG 3 cut(s) 1436, 2490, 4062
BfoI RGCGCY 1 cut(s) 1665
BfrI CTTAAG 5 cut(s) 98, 1777, 2444, 3358, 4016
BfuAI ACCTGC 1 cut(s) 212
BfuI GTATCC 1 cut(s) 864
BglII AGATCT 1 cut(s) 2341
Bme1390I CCNGG 3 cut(s) 1626, 2597, 2791
Bme18I GGWCC 2 cut(s) 2125, 3706
BmeT110I CYCGRG 1 cut(s) 377
BmgBI CACGTC 1 cut(s) 3916
BmgT120I GGNCC 3 cut(s) 2125, 2810, 3706
BmiI GGNNCC 4 cut(s) 153, 1218, 2274, 3846
BmrFI CCNGG 3 cut(s) 1626, 2597, 2791
BpiI GAAGAC 6 cut(s) 456, 489, 510, 696, 1131, 3918
BplI GAGNNNNNCTC 2 cut(s) 3221, 3253
BpmI CTGGAG 7 cut(s) 125, 1479, 2214, 2410, 3072, 3786, 3982
Bpu10I CCTNAGC 1 cut(s) 1069
BpuEI CTTGAG 2 cut(s) 68, 2724
BpuMI CCSGG 1 cut(s) 2791
BsaAI YACGTR 4 cut(s) 1542, 2309, 3135, 3881
BsaJI CCNNGG 5 cut(s) 724, 981, 1624, 2089, 3670
BsaWI WCCGGW 2 cut(s) 1263, 2856
Bsc4I CCNNNNNNNGG 7 cut(s) 1074, 1373, 1833, 2242, 2796, 2966, 4048
Bse118I RCCGGY 2 cut(s) 1263, 2856
Bse1I ACTGG 3 cut(s) 1112, 3529, 3832
Bse3DI GCAATG 3 cut(s) 2363, 2555, 3935
BseBI CCWGG 2 cut(s) 1626, 2597
BseDI CCNNGG 5 cut(s) 724, 981, 1624, 2089, 3670
BseGI GGATG 8 cut(s) 582, 838, 1204, 1860, 1988, 2652, 3316, 3569
BseLI CCNNNNNNNGG 7 cut(s) 1074, 1373, 1833, 2242, 2796, 2966, 4048
BseMI GCAATG 3 cut(s) 2363, 2555, 3935
BseMII CTCAG 1 cut(s) 2767
BseNI ACTGG 3 cut(s) 1112, 3529, 3832
BseRI GAGGAG 4 cut(s) 1826, 2217, 3788, 3789
BseSI GKGCMC 2 cut(s) 1294, 2887
BseXI GCAGC 8 cut(s) 1191, 1631, 1824, 1997, 2334, 2475, 2832, 3578
BsgI GTGCAG 1 cut(s) 732
Bsh1236I CGCG 1 cut(s) 3257
Bsh1285I CGRYCG 1 cut(s) 2790
BshFI GGCC 5 cut(s) 41, 1101, 1958, 2255, 2811
BshNI GGYRCC 1 cut(s) 3844
BshTI ACCGGT 2 cut(s) 1263, 2856
BsiEI CGRYCG 1 cut(s) 2790
BsiHKAI GWGCWC 4 cut(s) 111, 1008, 2157, 3738
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 4 cut(s) 1264, 2256, 2790, 2857
BslFI GGGAC 3 cut(s) 857, 1990, 3571
BslI CCNNNNNNNGG 7 cut(s) 1074, 1373, 1833, 2242, 2796, 2966, 4048
BsmAI GTCTC 2 cut(s) 406, 2762
BsmFI GGGAC 3 cut(s) 857, 1990, 3571
BsmI GAATGC 4 cut(s) 935, 1022, 1735, 3607
BsnI GGCC 5 cut(s) 41, 1101, 1958, 2255, 2811
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 6 cut(s) 111, 1008, 1294, 2157, 2887, 3738
BspACI CCGC 8 cut(s) 38, 71, 637, 1579, 2561, 2800, 3172, 3596
BspANI GGCC 5 cut(s) 41, 1101, 1958, 2255, 2811
BspCNI CTCAG 1 cut(s) 2766
BspFNI CGCG 1 cut(s) 3257
BspHI TCATGA 1 cut(s) 2715
BspLI GGNNCC 4 cut(s) 153, 1218, 2274, 3846
BspMI ACCTGC 1 cut(s) 212
BspPI GGATC 5 cut(s) 592, 668, 2607, 2657, 2978
BspQI GCTCTTC 1 cut(s) 1013
BspT107I GGYRCC 1 cut(s) 3844
BspTI CTTAAG 5 cut(s) 98, 1777, 2444, 3358, 4016
BsrDI GCAATG 3 cut(s) 2363, 2555, 3935
BsrFI RCCGGY 2 cut(s) 1263, 2856
BsrI ACTGG 3 cut(s) 1112, 3529, 3832
BssAI RCCGGY 2 cut(s) 1263, 2856
BssECI CCNNGG 5 cut(s) 724, 981, 1624, 2089, 3670
BssT1I CCWWGG 3 cut(s) 724, 981, 2089
Bst2UI CCWGG 2 cut(s) 1626, 2597
Bst4CI ACNGT 6 cut(s) 650, 667, 1571, 1587, 1871, 3452
Bst6I CTCTTC 5 cut(s) 732, 1013, 2184, 3371, 3765
BstAFI CTTAAG 5 cut(s) 98, 1777, 2444, 3358, 4016
BstAPI GCANNNNNTGC 2 cut(s) 2023, 3604
BstBAI YACGTR 4 cut(s) 1542, 2309, 3135, 3881
BstC8I GCNNGC 5 cut(s) 1433, 1503, 1666, 3026, 3096
BstDEI CTNAG 8 cut(s) 1069, 1241, 1997, 2524, 2753, 2834, 3059, 3578
BstEII GGTNACC 1 cut(s) 3527
BstENI CCTNNNNNAGG 1 cut(s) 2240
BstF5I GGATG 8 cut(s) 582, 838, 1204, 1860, 1988, 2652, 3316, 3569
BstFNI CGCG 1 cut(s) 3257
BstH2I RGCGCY 1 cut(s) 1665
BstHHI GCGC 3 cut(s) 1664, 1842, 3257
BstMAI GTCTC 2 cut(s) 406, 2762
BstMCI CGRYCG 1 cut(s) 2790
BstMWI GCNNNNNNNGC 5 cut(s) 1176, 2023, 2757, 2817, 3604
BstNI CCWGG 2 cut(s) 1626, 2597
BstNSI RCATGY 5 cut(s) 1435, 1476, 2115, 3028, 3069
BstPI GGTNACC 1 cut(s) 3527
BstSCI CCNGG 3 cut(s) 1624, 2595, 2789
BstSFI CTRYAG 3 cut(s) 1436, 2490, 4062
BstSLI GKGCMC 2 cut(s) 1294, 2887
BstSNI TACGTA 1 cut(s) 1542
BstUI CGCG 1 cut(s) 3257
BstV1I GCAGC 8 cut(s) 1191, 1631, 1824, 1997, 2334, 2475, 2832, 3578
BstV2I GAAGAC 6 cut(s) 456, 489, 510, 696, 1131, 3918
BstX2I RGATCY 2 cut(s) 2341, 2599
BstYI RGATCY 2 cut(s) 2341, 2599
BsuI GTATCC 1 cut(s) 864
BsuRI GGCC 5 cut(s) 41, 1101, 1958, 2255, 2811
BtgZI GCGATG 2 cut(s) 222, 2519
BtrI CACGTC 1 cut(s) 3916
BtsCI GGATG 8 cut(s) 582, 838, 1204, 1860, 1988, 2652, 3316, 3569
BtsIMutI CAGTG 3 cut(s) 663, 1119, 3522
BveI ACCTGC 1 cut(s) 212
Cac8I GCNNGC 5 cut(s) 1433, 1503, 1666, 3026, 3096
CaiI CAGNNNCTG 1 cut(s) 2014
CciI TCATGA 1 cut(s) 2715
CfoI GCGC 3 cut(s) 1664, 1842, 3257
Cfr10I RCCGGY 2 cut(s) 1263, 2856
Cfr13I GGNCC 3 cut(s) 2125, 2810, 3706
CseI GACGC 1 cut(s) 178
Csp6I GTAC 6 cut(s) 817, 1765, 2001, 2662, 3346, 3845
CspAI ACCGGT 2 cut(s) 1263, 2856
CviQI GTAC 6 cut(s) 817, 1765, 2001, 2662, 3346, 3845
DdeI CTNAG 8 cut(s) 1069, 1241, 1997, 2524, 2753, 2834, 3059, 3578
DraI TTTAAA 2 cut(s) 1788, 3369
EaeI YGGCCR 2 cut(s) 39, 2253
Eam1104I CTCTTC 5 cut(s) 732, 1013, 2184, 3371, 3765
EarI CTCTTC 5 cut(s) 732, 1013, 2184, 3371, 3765
EciI GGCGGA 3 cut(s) 1568, 2815, 3161
Ecl136II GAGCTC 4 cut(s) 109, 1006, 2155, 3736
Eco105I TACGTA 1 cut(s) 1542
Eco130I CCWWGG 3 cut(s) 724, 981, 2089
Eco147I AGGCCT 1 cut(s) 1101
Eco24I GRGCYC 4 cut(s) 111, 1008, 2157, 3738
Eco32I GATATC 2 cut(s) 395, 3108
Eco47I GGWCC 2 cut(s) 2125, 3706
Eco53kI GAGCTC 4 cut(s) 109, 1006, 2155, 3736
Eco57I CTGAAG 3 cut(s) 994, 1371, 2964
Eco88I CYCGRG 1 cut(s) 377
Eco91I GGTNACC 1 cut(s) 3527
EcoICRI GAGCTC 4 cut(s) 109, 1006, 2155, 3736
EcoNI CCTNNNNNAGG 1 cut(s) 2240
EcoO65I GGTNACC 1 cut(s) 3527
EcoRII CCWGG 2 cut(s) 1624, 2595
EcoRV GATATC 2 cut(s) 395, 3108
EcoT14I CCWWGG 3 cut(s) 724, 981, 2089
EcoT22I ATGCAT 4 cut(s) 1485, 2401, 3078, 3973
EcoT38I GRGCYC 4 cut(s) 111, 1008, 2157, 3738
ErhI CCWWGG 3 cut(s) 724, 981, 2089
FalI AAGNNNNNCTT 4 cut(s) 949, 981, 4028, 4060
FaqI GGGAC 3 cut(s) 857, 1990, 3571
FauNDI CATATG 2 cut(s) 2401, 3973
FbaI TGATCA 4 cut(s) 708, 1332, 2925, 3438
FblI GTMKAC 3 cut(s) 794, 1511, 2317
FokI GGATG 8 cut(s) 589, 845, 1191, 1847, 1975, 2639, 3323, 3556
FriOI GRGCYC 4 cut(s) 111, 1008, 2157, 3738
FspI TGCGCA 1 cut(s) 1841
GlaI GCGC 3 cut(s) 1663, 1841, 3256
GsuI CTGGAG 7 cut(s) 125, 1479, 2214, 2410, 3072, 3786, 3982
HaeII RGCGCY 1 cut(s) 1665
HaeIII GGCC 5 cut(s) 41, 1101, 1958, 2255, 2811
HapII CCGG 4 cut(s) 1264, 2256, 2790, 2857
HgaI GACGC 1 cut(s) 178
HhaI GCGC 3 cut(s) 1664, 1842, 3257
Hin6I GCGC 3 cut(s) 1662, 1840, 3255
HinP1I GCGC 3 cut(s) 1662, 1840, 3255
HincII GTYRAC 2 cut(s) 795, 1512
HindII GTYRAC 2 cut(s) 795, 1512
HindIII AAGCTT 5 cut(s) 996, 1637, 2441, 2721, 4013
HpaII CCGG 4 cut(s) 1264, 2256, 2790, 2857
HphI GGTGA 5 cut(s) 457, 754, 1583, 3539, 3776
HpyCH4III ACNGT 6 cut(s) 650, 667, 1571, 1587, 1871, 3452
HpyCH4IV ACGT 9 cut(s) 176, 815, 1541, 2308, 2586, 3134, 3880, 3892, 3915
HpyF10VI GCNNNNNNNGC 5 cut(s) 1176, 2023, 2757, 2817, 3604
HpyF3I CTNAG 8 cut(s) 1069, 1241, 1997, 2524, 2753, 2834, 3059, 3578
HpySE526I ACGT 9 cut(s) 176, 815, 1541, 2308, 2586, 3134, 3880, 3892, 3915
HspAI GCGC 3 cut(s) 1662, 1840, 3255
KpnI GGTACC 1 cut(s) 3848
Ksp22I TGATCA 4 cut(s) 708, 1332, 2925, 3438
LguI GCTCTTC 1 cut(s) 1013
Lsp1109I GCAGC 8 cut(s) 1191, 1631, 1824, 1997, 2334, 2475, 2832, 3578
MaeII ACGT 9 cut(s) 176, 815, 1541, 2308, 2586, 3134, 3880, 3892, 3915
MfeI CAATTG 5 cut(s) 752, 1368, 1737, 2961, 3318
MflI RGATCY 2 cut(s) 2341, 2599
MhlI GDGCHC 6 cut(s) 111, 1008, 1294, 2157, 2887, 3738
MlyI GAGTC 6 cut(s) 14, 259, 2129, 2639, 3710, 3790
Mph1103I ATGCAT 4 cut(s) 1485, 2401, 3078, 3973
MroXI GAANNNNTTC 5 cut(s) 125, 421, 1022, 1746, 2271
MslI CAYNNNNRTG 6 cut(s) 116, 246, 1159, 1673, 2311, 3221
MspA1I CMGCKG 1 cut(s) 11
MspCI CTTAAG 5 cut(s) 98, 1777, 2444, 3358, 4016
MspI CCGG 4 cut(s) 1264, 2256, 2790, 2857
MspR9I CCNGG 3 cut(s) 1626, 2597, 2791
MunI CAATTG 5 cut(s) 752, 1368, 1737, 2961, 3318
Mva1269I GAATGC 4 cut(s) 935, 1022, 1735, 3607
MvaI CCWGG 2 cut(s) 1626, 2597
MvnI CGCG 1 cut(s) 3257
MwoI GCNNNNNNNGC 5 cut(s) 1176, 2023, 2757, 2817, 3604
NciI CCSGG 1 cut(s) 2791
NdeI CATATG 2 cut(s) 2401, 3973
NlaIV GGNNCC 4 cut(s) 153, 1218, 2274, 3846
NmuCI GTSAC 9 cut(s) 742, 1378, 1865, 2114, 2132, 3527, 3695, 3713, 3823
NsbI TGCGCA 1 cut(s) 1841
NsiI ATGCAT 4 cut(s) 1485, 2401, 3078, 3973
NspI RCATGY 5 cut(s) 1435, 1476, 2115, 3028, 3069
OliI CACNNNNGTG 1 cut(s) 2311
PaeI GCATGC 2 cut(s) 1435, 3028
PagI TCATGA 1 cut(s) 2715
PceI AGGCCT 1 cut(s) 1101
PciSI GCTCTTC 1 cut(s) 1013
PctI GAATGC 4 cut(s) 935, 1022, 1735, 3607
PdmI GAANNNNTTC 5 cut(s) 125, 421, 1022, 1746, 2271
PflFI GACNNNGTC 1 cut(s) 2791
PflMI CCANNNNNTGG 2 cut(s) 1373, 2966
PinAI ACCGGT 2 cut(s) 1263, 2856
PleI GAGTC 6 cut(s) 13, 259, 2129, 2638, 3710, 3790
PpsI GAGTC 6 cut(s) 13, 259, 2129, 2638, 3710, 3790
Ppu21I YACGTR 4 cut(s) 1542, 2309, 3135, 3881
PshBI ATTAAT 3 cut(s) 1614, 3207, 3966
Psp124BI GAGCTC 4 cut(s) 111, 1008, 2157, 3738
Psp6I CCWGG 2 cut(s) 1624, 2595
PspEI GGTNACC 1 cut(s) 3527
PspGI CCWGG 2 cut(s) 1624, 2595
PspN4I GGNNCC 4 cut(s) 153, 1218, 2274, 3846
PspPI GGNCC 3 cut(s) 2125, 2810, 3706
PstNI CAGNNNCTG 1 cut(s) 2014
PsuI RGATCY 2 cut(s) 2341, 2599
PsyI GACNNNGTC 1 cut(s) 2791
PvuII CAGCTG 1 cut(s) 11
RsaI GTAC 6 cut(s) 818, 1766, 2002, 2663, 3347, 3846
RsaNI GTAC 6 cut(s) 817, 1765, 2001, 2662, 3346, 3845
RseI CAYNNNNRTG 6 cut(s) 116, 246, 1159, 1673, 2311, 3221
SacI GAGCTC 4 cut(s) 111, 1008, 2157, 3738
SalI GTCGAC 2 cut(s) 793, 1510
SapI GCTCTTC 1 cut(s) 1013
Sau96I GGNCC 3 cut(s) 2125, 2810, 3706
SchI GAGTC 6 cut(s) 14, 259, 2129, 2639, 3710, 3790
ScrFI CCNGG 3 cut(s) 1626, 2597, 2791
SduI GDGCHC 6 cut(s) 111, 1008, 1294, 2157, 2887, 3738
SfcI CTRYAG 3 cut(s) 1436, 2490, 4062
SinI GGWCC 2 cut(s) 2125, 3706
SmiI ATTTAAAT 2 cut(s) 1788, 3369
SmiMI CAYNNNNRTG 6 cut(s) 116, 246, 1159, 1673, 2311, 3221
SmlI CTYRAG 7 cut(s) 47, 98, 1777, 2444, 2739, 3358, 4016
SmoI CTYRAG 7 cut(s) 47, 98, 1777, 2444, 2739, 3358, 4016
SnaBI TACGTA 1 cut(s) 1542
SpeI ACTAGT 2 cut(s) 1759, 3340
SphI GCATGC 2 cut(s) 1435, 3028
SseBI AGGCCT 1 cut(s) 1101
SsiI CCGC 8 cut(s) 38, 71, 637, 1579, 2561, 2800, 3172, 3596
SstI GAGCTC 4 cut(s) 111, 1008, 2157, 3738
StuI AGGCCT 1 cut(s) 1101
StyD4I CCNGG 3 cut(s) 1624, 2595, 2789
StyI CCWWGG 3 cut(s) 724, 981, 2089
SwaI ATTTAAAT 2 cut(s) 1788, 3369
TaaI ACNGT 6 cut(s) 650, 667, 1571, 1587, 1871, 3452
TaiI ACGT 9 cut(s) 179, 818, 1544, 2311, 2589, 3137, 3883, 3895, 3918
TatI WGTACW 3 cut(s) 1764, 2000, 3345
TauI GCSGC 4 cut(s) 41, 74, 640, 2563
TscAI CASTG 3 cut(s) 670, 1119, 3529
TseFI GTSAC 9 cut(s) 742, 1378, 1865, 2114, 2132, 3527, 3695, 3713, 3823
TseI GCWGC 9 cut(s) 11, 1179, 1644, 1837, 1985, 2347, 2463, 2820, 3566
Tsp45I GTSAC 9 cut(s) 742, 1378, 1865, 2114, 2132, 3527, 3695, 3713, 3823
TspGWI ACGGA 3 cut(s) 1853, 2623, 3601
TspRI CASTG 3 cut(s) 670, 1119, 3529
Tth111I GACNNNGTC 1 cut(s) 2791
Van91I CCANNNNNTGG 2 cut(s) 1373, 2966
Vha464I CTTAAG 5 cut(s) 98, 1777, 2444, 3358, 4016
VpaK11BI GGWCC 2 cut(s) 2125, 3706
VspI ATTAAT 3 cut(s) 1614, 3207, 3966
XagI CCTNNNNNAGG 1 cut(s) 2240
XapI RAATTY 2 cut(s) 1789, 3370
XbaI TCTAGA 4 cut(s) 2327, 3298, 3331, 3899
XceI RCATGY 5 cut(s) 1435, 1476, 2115, 3028, 3069
XcmI CCANNNNNNNNNTGG 1 cut(s) 312
XmiI GTMKAC 3 cut(s) 794, 1511, 2317
XmnI GAANNNNTTC 5 cut(s) 125, 421, 1022, 1746, 2271
Zsp2I ATGCAT 4 cut(s) 1485, 2401, 3078, 3973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.