Belongs to the disease resistance NB-LRR family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 1223 |
| AccB1I | GGYRCC | 1 cut(s) | 1223 |
| AccI | GTMKAC | 1 cut(s) | 271 |
| AciI | CCGC | 2 cut(s) | 143, 775 |
| AclWI | GGATC | 1 cut(s) | 1167 |
| AcsI | RAATTY | 3 cut(s) | 233, 757, 1150 |
| AcuI | CTGAAG | 2 cut(s) | 186, 453 |
| AfaI | GTAC | 3 cut(s) | 398, 834, 1225 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1111 |
| AflIII | ACRYGT | 2 cut(s) | 246, 843 |
| AgsI | TTSAA | 9 cut(s) | 116, 218, 279, 509, 625, 763, 980, 1091, 1189 |
| AjiI | CACGTC | 1 cut(s) | 844 |
| AjnI | CCWGG | 2 cut(s) | 592, 825 |
| AluBI | AGCT | 6 cut(s) | 112, 128, 464, 711, 1009, 1185 |
| AluI | AGCT | 6 cut(s) | 112, 128, 464, 711, 1009, 1185 |
| Alw21I | GWGCWC | 3 cut(s) | 752, 1011, 1044 |
| Alw26I | GTCTC | 2 cut(s) | 192, 637 |
| AlwI | GGATC | 1 cut(s) | 1167 |
| AoxI | GGCC | 5 cut(s) | 3, 147, 204, 406, 1102 |
| ApeKI | GCWGC | 2 cut(s) | 938, 1168 |
| ApoI | RAATTY | 3 cut(s) | 233, 757, 1150 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 253, 285 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 128, 438 |
| Asp718I | GGTACC | 1 cut(s) | 1223 |
| AspS9I | GGNCC | 1 cut(s) | 590 |
| AsuHPI | GGTGA | 2 cut(s) | 649, 962 |
| AsuII | TTCGAA | 1 cut(s) | 706 |
| AvaII | GGWCC | 1 cut(s) | 590 |
| BanI | GGYRCC | 1 cut(s) | 1223 |
| BanII | GRGCYC | 1 cut(s) | 1011 |
| BbsI | GAAGAC | 3 cut(s) | 462, 495, 1134 |
| Bbv12I | GWGCWC | 3 cut(s) | 752, 1011, 1044 |
| BbvI | GCAGC | 2 cut(s) | 950, 1180 |
| BccI | CCATC | 1 cut(s) | 528 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 641, 675 |
| BciT130I | CCWGG | 2 cut(s) | 594, 827 |
| BclI | TGATCA | 1 cut(s) | 714 |
| BcoDI | GTCTC | 2 cut(s) | 192, 637 |
| BfaI | CTAG | 4 cut(s) | 134, 608, 836, 1217 |
| BisI | GCNGC | 3 cut(s) | 775, 939, 1169 |
| BlsI | GCNGC | 3 cut(s) | 776, 940, 1170 |
| Bme1390I | CCNGG | 2 cut(s) | 594, 827 |
| Bme18I | GGWCC | 1 cut(s) | 590 |
| BmgBI | CACGTC | 1 cut(s) | 844 |
| BmgT120I | GGNCC | 1 cut(s) | 590 |
| BmiI | GGNNCC | 1 cut(s) | 1225 |
| BmrFI | CCNGG | 2 cut(s) | 594, 827 |
| BmsI | GCATC | 3 cut(s) | 130, 201, 1204 |
| BpiI | GAAGAC | 3 cut(s) | 462, 495, 1134 |
| Bpu14I | TTCGAA | 1 cut(s) | 706 |
| BpuEI | CTTGAG | 1 cut(s) | 71 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1111 |
| Bse1I | ACTGG | 3 cut(s) | 75, 501, 1161 |
| Bse3DI | GCAATG | 1 cut(s) | 1211 |
| BseBI | CCWGG | 2 cut(s) | 594, 827 |
| BseGI | GGATG | 3 cut(s) | 145, 539, 550 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1111 |
| BseMI | GCAATG | 1 cut(s) | 1211 |
| BseMII | CTCAG | 2 cut(s) | 456, 910 |
| BseNI | ACTGG | 3 cut(s) | 75, 501, 1161 |
| BseRI | GAGGAG | 2 cut(s) | 122, 432 |
| BseXI | GCAGC | 2 cut(s) | 950, 1180 |
| BsgI | GTGCAG | 1 cut(s) | 473 |
| BshFI | GGCC | 5 cut(s) | 5, 149, 206, 408, 1104 |
| BshNI | GGYRCC | 1 cut(s) | 1223 |
| BsiHKAI | GWGCWC | 3 cut(s) | 752, 1011, 1044 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1111 |
| BsmAI | GTCTC | 2 cut(s) | 192, 637 |
| BsmI | GAATGC | 2 cut(s) | 769, 1025 |
| BsnI | GGCC | 5 cut(s) | 5, 149, 206, 408, 1104 |
| Bsp119I | TTCGAA | 1 cut(s) | 706 |
| Bsp1286I | GDGCHC | 3 cut(s) | 752, 1011, 1044 |
| Bsp143I | GATC | 3 cut(s) | 366, 714, 1159 |
| BspACI | CCGC | 2 cut(s) | 143, 775 |
| BspANI | GGCC | 5 cut(s) | 5, 149, 206, 408, 1104 |
| BspCNI | CTCAG | 2 cut(s) | 457, 909 |
| BspLI | GGNNCC | 1 cut(s) | 1225 |
| BspPI | GGATC | 1 cut(s) | 1167 |
| BspQI | GCTCTTC | 2 cut(s) | 543, 1016 |
| BspT104I | TTCGAA | 1 cut(s) | 706 |
| BspT107I | GGYRCC | 1 cut(s) | 1223 |
| BsrDI | GCAATG | 1 cut(s) | 1211 |
| BsrI | ACTGG | 3 cut(s) | 75, 501, 1161 |
| BssMI | GATC | 3 cut(s) | 366, 714, 1159 |
| Bst2UI | CCWGG | 2 cut(s) | 594, 827 |
| Bst4CI | ACNGT | 5 cut(s) | 401, 565, 629, 974, 1223 |
| Bst6I | CTCTTC | 4 cut(s) | 543, 635, 738, 1016 |
| BstBI | TTCGAA | 1 cut(s) | 706 |
| BstC8I | GCNNGC | 1 cut(s) | 190 |
| BstDEI | CTNAG | 2 cut(s) | 465, 896 |
| BstF5I | GGATG | 3 cut(s) | 145, 539, 550 |
| BstKTI | GATC | 3 cut(s) | 369, 717, 1162 |
| BstMAI | GTCTC | 2 cut(s) | 192, 637 |
| BstMBI | GATC | 3 cut(s) | 366, 714, 1159 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 155, 1121 |
| BstNI | CCWGG | 2 cut(s) | 594, 827 |
| BstNSI | RCATGY | 1 cut(s) | 250 |
| BstSCI | CCNGG | 2 cut(s) | 592, 825 |
| BstV1I | GCAGC | 2 cut(s) | 950, 1180 |
| BstV2I | GAAGAC | 3 cut(s) | 462, 495, 1134 |
| BsuRI | GGCC | 5 cut(s) | 5, 149, 206, 408, 1104 |
| BtrI | CACGTC | 1 cut(s) | 844 |
| BtsCI | GGATG | 3 cut(s) | 145, 539, 550 |
| BtsIMutI | CAGTG | 3 cut(s) | 82, 634, 1154 |
| Cac8I | GCNNGC | 1 cut(s) | 190 |
| Cfr13I | GGNCC | 1 cut(s) | 590 |
| CsiI | ACCWGGT | 1 cut(s) | 825 |
| Csp6I | GTAC | 3 cut(s) | 397, 833, 1224 |
| CviAII | CATG | 7 cut(s) | 121, 247, 541, 583, 663, 769, 965 |
| CviQI | GTAC | 3 cut(s) | 397, 833, 1224 |
| DdeI | CTNAG | 2 cut(s) | 465, 896 |
| DpnI | GATC | 3 cut(s) | 368, 716, 1161 |
| DpnII | GATC | 3 cut(s) | 366, 714, 1159 |
| Eam1104I | CTCTTC | 4 cut(s) | 543, 635, 738, 1016 |
| EarI | CTCTTC | 4 cut(s) | 543, 635, 738, 1016 |
| Ecl136II | GAGCTC | 1 cut(s) | 1009 |
| Eco147I | AGGCCT | 1 cut(s) | 1104 |
| Eco24I | GRGCYC | 1 cut(s) | 1011 |
| Eco32I | GATATC | 1 cut(s) | 223 |
| Eco47I | GGWCC | 1 cut(s) | 590 |
| Eco53kI | GAGCTC | 1 cut(s) | 1009 |
| Eco57I | CTGAAG | 2 cut(s) | 186, 453 |
| EcoICRI | GAGCTC | 1 cut(s) | 1009 |
| EcoO109I | RGGNCCY | 1 cut(s) | 590 |
| EcoRI | GAATTC | 2 cut(s) | 233, 1150 |
| EcoRII | CCWGG | 2 cut(s) | 592, 825 |
| EcoRV | GATATC | 1 cut(s) | 223 |
| EcoT22I | ATGCAT | 1 cut(s) | 664 |
| EcoT38I | GRGCYC | 1 cut(s) | 1011 |
| FaeI | CATG | 7 cut(s) | 124, 250, 544, 586, 666, 772, 968 |
| FatI | CATG | 7 cut(s) | 120, 246, 540, 582, 662, 768, 964 |
| FbaI | TGATCA | 1 cut(s) | 714 |
| FblI | GTMKAC | 1 cut(s) | 271 |
| Fnu4HI | GCNGC | 3 cut(s) | 775, 939, 1169 |
| FokI | GGATG | 3 cut(s) | 152, 546, 557 |
| FriOI | GRGCYC | 1 cut(s) | 1011 |
| Fsp4HI | GCNGC | 3 cut(s) | 775, 939, 1169 |
| FspBI | CTAG | 4 cut(s) | 134, 608, 836, 1217 |
| GluI | GCNGC | 3 cut(s) | 775, 939, 1169 |
| HaeIII | GGCC | 5 cut(s) | 5, 149, 206, 408, 1104 |
| Hin1II | CATG | 7 cut(s) | 124, 250, 544, 586, 666, 772, 968 |
| HincII | GTYRAC | 1 cut(s) | 272 |
| HindII | GTYRAC | 1 cut(s) | 272 |
| HindIII | AAGCTT | 2 cut(s) | 126, 709 |
| HinfI | GANTC | 5 cut(s) | 268, 388, 467, 812, 906 |
| HphI | GGTGA | 2 cut(s) | 649, 962 |
| Hpy166II | GTNNAC | 3 cut(s) | 272, 970, 1055 |
| Hpy188I | TCNGA | 3 cut(s) | 197, 433, 899 |
| Hpy188III | TCNNGA | 3 cut(s) | 50, 83, 380 |
| Hpy8I | GTNNAC | 3 cut(s) | 272, 970, 1055 |
| HpyAV | CCTTC | 5 cut(s) | 53, 161, 503, 631, 733 |
| HpyCH4III | ACNGT | 5 cut(s) | 401, 565, 629, 974, 1223 |
| HpyCH4IV | ACGT | 3 cut(s) | 328, 683, 843 |
| HpyCH4V | TGCA | 8 cut(s) | 188, 338, 454, 662, 755, 1017, 1179, 1201 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 155, 1121 |
| HpyF3I | CTNAG | 2 cut(s) | 465, 896 |
| HpySE526I | ACGT | 3 cut(s) | 328, 683, 843 |
| Hsp92II | CATG | 7 cut(s) | 124, 250, 544, 586, 666, 772, 968 |
| KpnI | GGTACC | 1 cut(s) | 1227 |
| Ksp22I | TGATCA | 1 cut(s) | 714 |
| Kzo9I | GATC | 3 cut(s) | 366, 714, 1159 |
| LguI | GCTCTTC | 2 cut(s) | 543, 1016 |
| LmnI | GCTCC | 3 cut(s) | 109, 419, 1006 |
| Lsp1109I | GCAGC | 2 cut(s) | 950, 1180 |
| LweI | GCATC | 3 cut(s) | 130, 201, 1204 |
| MabI | ACCWGGT | 1 cut(s) | 825 |
| MaeI | CTAG | 4 cut(s) | 134, 608, 836, 1217 |
| MaeII | ACGT | 3 cut(s) | 328, 683, 843 |
| MaeIII | GTNAC | 3 cut(s) | 13, 345, 792 |
| MalI | GATC | 3 cut(s) | 368, 716, 1161 |
| MboI | GATC | 3 cut(s) | 366, 714, 1159 |
| MhlI | GDGCHC | 3 cut(s) | 752, 1011, 1044 |
| MlyI | GAGTC | 3 cut(s) | 277, 476, 821 |
| MmeI | TCCRAC | 1 cut(s) | 840 |
| Mph1103I | ATGCAT | 1 cut(s) | 664 |
| MroXI | GAANNNNTTC | 2 cut(s) | 128, 438 |
| MseI | TTAA | 5 cut(s) | 26, 102, 801, 957, 1073 |
| MslI | CAYNNNNRTG | 1 cut(s) | 249 |
| MspR9I | CCNGG | 2 cut(s) | 594, 827 |
| Mva1269I | GAATGC | 2 cut(s) | 769, 1025 |
| MvaI | CCWGG | 2 cut(s) | 594, 827 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 155, 1121 |
| NdeII | GATC | 3 cut(s) | 366, 714, 1159 |
| NlaIII | CATG | 7 cut(s) | 124, 250, 544, 586, 666, 772, 968 |
| NlaIV | GGNNCC | 1 cut(s) | 1225 |
| NsiI | ATGCAT | 1 cut(s) | 664 |
| NspI | RCATGY | 1 cut(s) | 250 |
| NspV | TTCGAA | 1 cut(s) | 706 |
| PceI | AGGCCT | 1 cut(s) | 1104 |
| PciI | ACATGT | 1 cut(s) | 246 |
| PciSI | GCTCTTC | 2 cut(s) | 543, 1016 |
| PctI | GAATGC | 2 cut(s) | 769, 1025 |
| PdmI | GAANNNNTTC | 2 cut(s) | 128, 438 |
| PfeI | GAWTC | 2 cut(s) | 388, 906 |
| PfoI | TCCNGGA | 1 cut(s) | 592 |
| PkrI | GCNGC | 3 cut(s) | 776, 940, 1170 |
| PleI | GAGTC | 3 cut(s) | 276, 475, 820 |
| PpsI | GAGTC | 3 cut(s) | 276, 475, 820 |
| PpuMI | RGGWCCY | 1 cut(s) | 590 |
| PscI | ACATGT | 1 cut(s) | 246 |
| Psp124BI | GAGCTC | 1 cut(s) | 1011 |
| Psp5II | RGGWCCY | 1 cut(s) | 590 |
| Psp6I | CCWGG | 2 cut(s) | 592, 825 |
| PspGI | CCWGG | 2 cut(s) | 592, 825 |
| PspN4I | GGNNCC | 1 cut(s) | 1225 |
| PspPI | GGNCC | 1 cut(s) | 590 |
| PspPPI | RGGWCCY | 1 cut(s) | 590 |
| RsaI | GTAC | 3 cut(s) | 398, 834, 1225 |
| RsaNI | GTAC | 3 cut(s) | 397, 833, 1224 |
| RseI | CAYNNNNRTG | 1 cut(s) | 249 |
| SacI | GAGCTC | 1 cut(s) | 1011 |
| SalI | GTCGAC | 1 cut(s) | 270 |
| SapI | GCTCTTC | 2 cut(s) | 543, 1016 |
| SaqAI | TTAA | 5 cut(s) | 26, 102, 801, 957, 1073 |
| SatI | GCNGC | 3 cut(s) | 775, 939, 1169 |
| Sau3AI | GATC | 3 cut(s) | 366, 714, 1159 |
| Sau96I | GGNCC | 1 cut(s) | 590 |
| SchI | GAGTC | 3 cut(s) | 277, 476, 821 |
| ScrFI | CCNGG | 2 cut(s) | 594, 827 |
| SduI | GDGCHC | 3 cut(s) | 752, 1011, 1044 |
| SexAI | ACCWGGT | 1 cut(s) | 825 |
| SfaNI | GCATC | 3 cut(s) | 130, 201, 1204 |
| SfuI | TTCGAA | 1 cut(s) | 706 |
| SinI | GGWCC | 1 cut(s) | 590 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 249 |
| SmlI | CTYRAG | 1 cut(s) | 50 |
| SmoI | CTYRAG | 1 cut(s) | 50 |
| SseBI | AGGCCT | 1 cut(s) | 1104 |
| SsiI | CCGC | 2 cut(s) | 143, 775 |
| SspMI | CTAG | 4 cut(s) | 134, 608, 836, 1217 |
| SstI | GAGCTC | 1 cut(s) | 1011 |
| StuI | AGGCCT | 1 cut(s) | 1104 |
| StyD4I | CCNGG | 2 cut(s) | 592, 825 |
| TaaI | ACNGT | 5 cut(s) | 401, 565, 629, 974, 1223 |
| TaiI | ACGT | 3 cut(s) | 331, 686, 846 |
| TaqI | TCGA | 5 cut(s) | 271, 651, 706, 904, 922 |
| TatI | WGTACW | 1 cut(s) | 832 |
| TauI | GCSGC | 1 cut(s) | 777 |
| TfiI | GAWTC | 2 cut(s) | 388, 906 |
| Tru1I | TTAA | 5 cut(s) | 26, 102, 801, 957, 1073 |
| Tru9I | TTAA | 5 cut(s) | 26, 102, 801, 957, 1073 |
| TscAI | CASTG | 3 cut(s) | 82, 634, 1161 |
| TseI | GCWGC | 2 cut(s) | 938, 1168 |
| TspDTI | ATGAA | 7 cut(s) | 102, 137, 226, 246, 469, 561, 1200 |
| TspRI | CASTG | 3 cut(s) | 82, 634, 1161 |
| VpaK11BI | GGWCC | 1 cut(s) | 590 |
| XapI | RAATTY | 3 cut(s) | 233, 757, 1150 |
| XceI | RCATGY | 1 cut(s) | 250 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 315, 823, 1153 |
| XmiI | GTMKAC | 1 cut(s) | 271 |
| XmnI | GAANNNNTTC | 2 cut(s) | 128, 438 |
| XspI | CTAG | 4 cut(s) | 134, 608, 836, 1217 |
| Zsp2I | ATGCAT | 1 cut(s) | 664 |