Prupe.8G009400_v2.0.a1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000110
Physical Location & Seq
Reverse (-)
692008 .. 695560
3553 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G009400.1

Sequence Viewer

Length: 2754 bp
ATGGCCTTGCCAACAGACCTCCTGATTGGCAAAATTGTGGCCATTTTTGAGAACGAAGCATCCTCCATAGCTGGTGTTCGTGATGAAATTGATGACATCAAGCAGGAGCTTGTAAGCATGAAGATCTTCCTAAATGATTTTGAGGGCAAGAAGGCACTCACCGAAGGTGGGGAGACGTGGGTTGCAAGCGTCAGACGCATGGCCTATGATGTTGAAGACATCATTGACGAGTTCATGTATCACATGTATGAGCGAGGATGTCATGATGGCCGATTTGCAAGGTGGTTCCACCAAACTATTCGCATTCCACCGAATGTTTGGTTCAGACGTCAAATGTCAAAGAAGTTGCGGAAAATCTCAAGAAGGATTAAAGCCATTCCGGAGAGGAATCAGAGATATGGTGTCGGTGGATTAGAAGGAACAACTAGTACTTGTGATGTTGGCAAATGGATGCGGAACCAAGCAGAATCTTCTCTTTTTATTAAGGAAGATGAGCTCGTGGGGATTGAAAGAAAGAAGCAACTATTGATGAATTGGTTGATGAATGGAGAGCAACAGCAAACGGTTATCTCTGTGGTGGGGATGGGCGGATCAGGGAAGACGACTTTAGTTGCCAAGACCTTCAACGACGAAAGGGTCAAGAAAAAATTCCACTGTTGTGCTTGGCTAACTGTTTCACAAACTTACGTGATTGACGACTTGTTTAGAAGCTTGATTAAGGAATTCCACGAAGCAAGGATGGAAAAGGTCCCTGCAGACATGAATTCCATGACATATAGAGAATTGCTACAGGTGCTGGTCAATTACTTGGAGTCTAAAAGGTACATGGTTGTATTGGATGATGTGTGGGATATCAAACTTTGGAAAGAAATGAGGATAGCACTCCCAAATACACAGTTTGGAAGTCGAATCATGCTTACTACTCGAAGAGAAGACGTAGCATCCTATTGTTTTGGAGTACAAAGTCATATTCACTACATTCAACCCCTCGAAAAGAATGACGCTTGGGAGCTATTCAGCAGCAAAGCATTCTCAGCTTACCAGAATAAATGTTGTCCACTAGATCTTCAGTCATTGGCTGAGGAACTTGTAGAAAAGTGTGAAGGCCTACCTCTGGCAGTCGTGGCTTTAGGTGGTCTGATGTCCTCCAAGAAGCCATTCGAGTGGAAGCAAGTCTACAACAGCTTAAACTTGCATTTAACTAACCATCCGTTGCTAGAGCCAGTGAAGAGCATCTTGTTGCTTAGTTTTGATGATTTACCATACCCATTGAAGCACTGTTTTCTGTACTGTTCCCTTTTCCCAGAAGACTATTCGATTCGAAGAAAAAGGCTCATTAGGTTGTGGATAGCTGAAGGGTTTGTTCAGGATGGAAAAGGCGCCACACCAGAAGAAGTTGCTGAGAGCTATCTTATGCAGCTCATTTTCCGCAGCATGCTACAGGTTGTTCAGAGGAATGAAAGTGGAAGGCCAAAAGCATGTAAGATGCACGACCTTATGCTGGAGCTTGCTCTGTCTAAATCAGAAAAAGAAAAGTTTGGTGCTGTATATGACGGAAAAGAAGTTATGGATGAAGGTCAAGTGCGCCGCTTGTCAACTCAAACCACTGGAGGAGAAATTAAACTAGGCACTGGTATGGCACAACTTCGTTCTTTTCTTATGTTTGTTAGTGACGTGTCCTCATCCTCTTCCTCGAATACATTGCCTTCTGGATGCAAATTGTTGAGGGTGCTAGATTTGCAATATGTTCCAATTGATATACTGCCAAAAGAACTTGCGTACTTATTCAATTTAAGATACTTAAATTTAAGGGGAACTCCAGTTAAGAAGCTTCCTGAATCCATTGGAAAGCTCCGCAACCTTCAAACTTTGGATATCAGGGACTCAAAGATAGAGGTACTTCCAAGTGGAATTGCCAAGTTGCAAAACTTGCGCCATCTAATTATGTATCGTCGCACTGAAGAGCATAGGGGCTTCAGATATGTGAATGGGACAAGATCACCATCAAATATATGTATGTTGAAGAAATTGCAAGTTTTGGCTTGTGTTGAATTAGAAGGAAAGATTGTTAGACTTGTGGGGAATATGACCCAACTTAGAAGGATTGGAATTACGAATGTGAAAGAAAGAGACGAGATGGACCTATGTGCCTCAATTCGAAAGATGAAGCAACTTCACTACTTGTTTCTAATGACAAGCGATGAAGAGGAAGTTCTTCAAACAAACACACTGTGCTCACCTCCTCCCCATCTTCGAACAGTTATTTTGAATGGAAAATTGGAAAAGGTACCACGTTGGGTCTCCTCACTCCAGAGCCTCACACATTTGAATCTGATTTGGTCTGGAATTGAAGAAGATTTACTACCTTACATTGAAGCACTGCCCAATCTGGGAAGGCTTACACTTGTTAATGCATATGCTGGCAGAGAGTTATGTTTCAGCAGAGGCTTTGCAAAGCTTACGAAATTGGAATTGTCTATTTGCCACTTATTGAACAAGGTAACTATAGAAAAAGGGGTGATGTCAAATCTCCAATTCTTATGCCTTGAAAACTGCCCAGAACTAAACACAATGCCACAGGGTCTTGAATACCTCACTGAACTAAAAGTATTGACATTGGTGGTTGTATCAAAGGAACTCAAGGATTCCATACAAGAAGGAGGTGTGGATCATGAAAAGGTACAACACATCCCTGAGATCTACCATTATTACAAATCGTCACTGGGGATGATGTGTCGTGAAAGCTTGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

918

Amino Acids

105.24

Weight (kDa)

8.57

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 635
AatII GACGTC 1 cut(s) 331
Acc65I GGTACC 1 cut(s) 2287
AccB1I GGYRCC 2 cut(s) 1379, 2287
AccI GTMKAC 1 cut(s) 1176
AccIII TCCGGA 1 cut(s) 379
AciI CCGC 6 cut(s) 349, 454, 588, 1429, 1588, 1855
AclWI GGATC 2 cut(s) 598, 2676
AcoI YGGCCR 2 cut(s) 39, 268
AcsI RAATTY 4 cut(s) 647, 722, 763, 1804
AcuI CTGAAG 4 cut(s) 1052, 1374, 1960, 1980
AcyI GRCGYC 2 cut(s) 328, 1380
AdeI CACNNNGTG 1 cut(s) 2232
AfaI GTAC 8 cut(s) 430, 824, 960, 1289, 1781, 1899, 2289, 2682
AfiI CCNNNNNNNGG 4 cut(s) 168, 1114, 1501, 2390
AflIII ACRYGT 2 cut(s) 243, 1674
AhlI ACTAGT 1 cut(s) 425
AjiI CACGTC 2 cut(s) 177, 1675
AjuI GAANNNNNNNTTGG 4 cut(s) 1142, 1174, 2261, 2293
AleI CACNNNNGTG 1 cut(s) 657
AloI GAACNNNNNNTCC 2 cut(s) 44, 76
Alw21I GWGCWC 2 cut(s) 498, 2237
Alw26I GTCTC 3 cut(s) 167, 2124, 2305
AlwI GGATC 2 cut(s) 598, 2676
AlwNI CAGNNNCTG 1 cut(s) 796
Aor13HI TCCGGA 1 cut(s) 379
AoxI GGCC 6 cut(s) 3, 39, 201, 268, 1105, 1469
ApeKI GCWGC 3 cut(s) 1020, 1417, 1431
ApoI RAATTY 4 cut(s) 647, 722, 763, 1804
Asp700I GAANNNNTTC 4 cut(s) 125, 647, 1157, 2214
Asp718I GGTACC 1 cut(s) 2287
AspLEI GCGC 3 cut(s) 1382, 1587, 1935
AspS9I GGNCC 2 cut(s) 748, 2140
AsuHPI GGTGA 4 cut(s) 151, 1992, 2229, 2530
AsuII TTCGAA 3 cut(s) 1321, 2158, 2254
AvaII GGWCC 2 cut(s) 748, 2140
BalI TGGCCA 1 cut(s) 41
BanI GGYRCC 2 cut(s) 1379, 2287
BanII GRGCYC 1 cut(s) 498
BarI GAAGNNNNNNTAC 6 cut(s) 1160, 1192, 2343, 2346, 2375, 2378
BauI CACGAG 1 cut(s) 497
BbsI GAAGAC 4 cut(s) 222, 605, 939, 1314
Bbv12I GWGCWC 2 cut(s) 498, 2237
BbvCI CCTCAGC 1 cut(s) 1080
BbvI GCAGC 3 cut(s) 1032, 1429, 1443
BccI CCATC 9 cut(s) 260, 577, 733, 1215, 1364, 1944, 2011, 2131, 2256
BcgI CGANNNNNNTGC 2 cut(s) 1684, 1718
BcoDI GTCTC 3 cut(s) 167, 2124, 2305
BcuI ACTAGT 1 cut(s) 425
BfaI CTAG 6 cut(s) 426, 1061, 1217, 1625, 1733, 2752
BfmI CTRYAG 4 cut(s) 753, 788, 1439, 2505
BfoI RGCGCY 1 cut(s) 1383
BglII AGATCT 3 cut(s) 123, 1063, 2697
BisI GCNGC 4 cut(s) 1021, 1418, 1432, 1588
BlsI GCNGC 4 cut(s) 1022, 1419, 1433, 1589
BmcAI AGTACT 1 cut(s) 430
Bme18I GGWCC 2 cut(s) 748, 2140
BmgBI CACGTC 2 cut(s) 177, 1675
BmgT120I GGNCC 2 cut(s) 748, 2140
BmiI GGNNCC 5 cut(s) 287, 458, 750, 1381, 2289
BmrI ACTGGG 1 cut(s) 2732
BmsI GCATC 6 cut(s) 68, 441, 950, 1242, 1476, 1703
BmuI ACTGGG 1 cut(s) 2732
BpiI GAAGAC 4 cut(s) 222, 605, 939, 1314
BpmI CTGGAG 4 cut(s) 1523, 1629, 1803, 2294
Bpu10I CCTNAGC 1 cut(s) 1080
Bpu14I TTCGAA 3 cut(s) 1321, 2158, 2254
BpuEI CTTGAG 2 cut(s) 343, 2624
BsaAI YACGTR 1 cut(s) 688
BsaBI GATNNNNATC 1 cut(s) 2002
BsaHI GRCGYC 2 cut(s) 328, 1380
BsaI GGTCTC 1 cut(s) 2305
BsaWI WCCGGW 1 cut(s) 379
Bsc4I CCNNNNNNNGG 4 cut(s) 168, 1114, 1501, 2390
Bse1I ACTGG 5 cut(s) 1223, 1612, 1636, 1820, 2727
Bse3DI GCAATG 1 cut(s) 1700
Bse8I GATNNNNATC 1 cut(s) 2002
BseAI TCCGGA 1 cut(s) 379
BseJI GATNNNNATC 1 cut(s) 2002
BseLI CCNNNNNNNGG 4 cut(s) 168, 1114, 1501, 2390
BseMI GCAATG 1 cut(s) 1700
BseMII CTCAG 4 cut(s) 1047, 1071, 1392, 2685
BseNI ACTGG 5 cut(s) 1223, 1612, 1636, 1820, 2727
BseRI GAGGAG 3 cut(s) 1626, 2232, 2293
BseXI GCAGC 3 cut(s) 1032, 1429, 1443
BshFI GGCC 6 cut(s) 5, 41, 203, 270, 1107, 1471
BshNI GGYRCC 2 cut(s) 1379, 2287
BsiHKAI GWGCWC 2 cut(s) 498, 2237
BsiSI CCGG 1 cut(s) 380
BslFI GGGAC 3 cut(s) 734, 1895, 2005
BslI CCNNNNNNNGG 4 cut(s) 168, 1114, 1501, 2390
BsmAI GTCTC 3 cut(s) 167, 2124, 2305
BsmBI CGTCTC 2 cut(s) 167, 2124
BsmFI GGGAC 3 cut(s) 734, 1895, 2005
BsmI GAATGC 2 cut(s) 303, 1028
BsnI GGCC 6 cut(s) 5, 41, 203, 270, 1107, 1471
Bso31I GGTCTC 1 cut(s) 2305
Bsp119I TTCGAA 3 cut(s) 1321, 2158, 2254
Bsp1286I GDGCHC 2 cut(s) 498, 2237
Bsp13I TCCGGA 1 cut(s) 379
Bsp143I GATC 6 cut(s) 123, 590, 1063, 1997, 2668, 2697
BspACI CCGC 6 cut(s) 349, 454, 588, 1429, 1588, 1855
BspANI GGCC 6 cut(s) 5, 41, 203, 270, 1107, 1471
BspCNI CTCAG 4 cut(s) 1046, 1072, 1393, 2686
BspEI TCCGGA 1 cut(s) 379
BspHI TCATGA 2 cut(s) 262, 2671
BspLI GGNNCC 5 cut(s) 287, 458, 750, 1381, 2289
BspMAI CTGCAG 1 cut(s) 757
BspPI GGATC 2 cut(s) 598, 2676
BspQI GCTCTTC 2 cut(s) 1223, 1956
BspT104I TTCGAA 3 cut(s) 1321, 2158, 2254
BspT107I GGYRCC 2 cut(s) 1379, 2287
BspTNI GGTCTC 1 cut(s) 2305
BsrDI GCAATG 1 cut(s) 1700
BsrI ACTGG 5 cut(s) 1223, 1612, 1636, 1820, 2727
BssMI GATC 6 cut(s) 123, 590, 1063, 1997, 2668, 2697
BssNI GRCGYC 2 cut(s) 328, 1380
BssSI CACGAG 1 cut(s) 497
Bst2BI CACGAG 1 cut(s) 497
Bst4CI ACNGT 8 cut(s) 565, 656, 673, 897, 1280, 1292, 2232, 2260
Bst6I CTCTTC 5 cut(s) 922, 1223, 1693, 1956, 2199
BstACI GRCGYC 2 cut(s) 328, 1380
BstAPI GCANNNNNTGC 1 cut(s) 1930
BstBAI YACGTR 1 cut(s) 688
BstBI TTCGAA 3 cut(s) 1321, 2158, 2254
BstC8I GCNNGC 4 cut(s) 187, 1436, 1509, 2422
BstDEI CTNAG 6 cut(s) 1033, 1080, 1244, 1401, 2096, 2694
BstH2I RGCGCY 1 cut(s) 1383
BstHHI GCGC 3 cut(s) 1382, 1587, 1935
BstKTI GATC 6 cut(s) 126, 593, 1066, 2000, 2671, 2700
BstMAI GTCTC 3 cut(s) 167, 2124, 2305
BstMBI GATC 6 cut(s) 123, 590, 1063, 1997, 2668, 2697
BstMWI GCNNNNNNNGC 6 cut(s) 195, 793, 1034, 1124, 1738, 1930
BstNSI RCATGY 3 cut(s) 247, 1438, 1482
BstSFI CTRYAG 4 cut(s) 753, 788, 1439, 2505
BstV1I GCAGC 3 cut(s) 1032, 1429, 1443
BstV2I GAAGAC 4 cut(s) 222, 605, 939, 1314
BstX2I RGATCY 3 cut(s) 123, 1063, 2697
BstXI CCANNNNNNTGG 1 cut(s) 1164
BstYI RGATCY 3 cut(s) 123, 1063, 2697
BsuRI GGCC 6 cut(s) 5, 41, 203, 270, 1107, 1471
BtgZI GCGATG 1 cut(s) 2214
BtrI CACGTC 2 cut(s) 177, 1675
BtsI GCAGTG 1 cut(s) 2378
Cac8I GCNNGC 4 cut(s) 187, 1436, 1509, 2422
CaiI CAGNNNCTG 1 cut(s) 796
CciI TCATGA 2 cut(s) 262, 2671
CfoI GCGC 3 cut(s) 1382, 1587, 1935
Cfr13I GGNCC 2 cut(s) 748, 2140
CseI GACGC 3 cut(s) 178, 204, 1010
Csp6I GTAC 8 cut(s) 429, 823, 959, 1288, 1780, 1898, 2288, 2681
CviQI GTAC 8 cut(s) 429, 823, 959, 1288, 1780, 1898, 2288, 2681
DdeI CTNAG 6 cut(s) 1033, 1080, 1244, 1401, 2096, 2694
DinI GGCGCC 1 cut(s) 1381
DpnI GATC 6 cut(s) 125, 592, 1065, 1999, 2670, 2699
DpnII GATC 6 cut(s) 123, 590, 1063, 1997, 2668, 2697
DraIII CACNNNGTG 1 cut(s) 2232
DrdI GACNNNNNNGTC 1 cut(s) 635
DseDI GACNNNNNNGTC 1 cut(s) 635
EaeI YGGCCR 2 cut(s) 39, 268
Eam1104I CTCTTC 5 cut(s) 922, 1223, 1693, 1956, 2199
EarI CTCTTC 5 cut(s) 922, 1223, 1693, 1956, 2199
EciI GGCGGA 1 cut(s) 603
Ecl136II GAGCTC 1 cut(s) 496
Eco147I AGGCCT 1 cut(s) 1107
Eco24I GRGCYC 1 cut(s) 498
Eco31I GGTCTC 1 cut(s) 2305
Eco32I GATATC 2 cut(s) 853, 1876
Eco47I GGWCC 2 cut(s) 748, 2140
Eco53kI GAGCTC 1 cut(s) 496
Eco57I CTGAAG 4 cut(s) 1052, 1374, 1960, 1980
EcoICRI GAGCTC 1 cut(s) 496
EcoO109I RGGNCCY 1 cut(s) 748
EcoRI GAATTC 2 cut(s) 722, 763
EcoRV GATATC 2 cut(s) 853, 1876
EcoT22I ATGCAT 1 cut(s) 2416
EcoT38I GRGCYC 1 cut(s) 498
EgeI GGCGCC 1 cut(s) 1381
EheI GGCGCC 1 cut(s) 1381
Esp3I CGTCTC 2 cut(s) 167, 2124
FalI AAGNNNNNCTT 2 cut(s) 1220, 1252
FaqI GGGAC 3 cut(s) 734, 1895, 2005
FauNDI CATATG 1 cut(s) 2416
FblI GTMKAC 1 cut(s) 1176
Fnu4HI GCNGC 4 cut(s) 1021, 1418, 1432, 1588
FriOI GRGCYC 1 cut(s) 498
Fsp4HI GCNGC 4 cut(s) 1021, 1418, 1432, 1588
FspBI CTAG 6 cut(s) 426, 1061, 1217, 1625, 1733, 2752
GlaI GCGC 3 cut(s) 1381, 1586, 1934
GluI GCNGC 4 cut(s) 1021, 1418, 1432, 1588
GsuI CTGGAG 4 cut(s) 1523, 1629, 1803, 2294
HaeII RGCGCY 1 cut(s) 1383
HaeIII GGCC 6 cut(s) 5, 41, 203, 270, 1107, 1471
HapII CCGG 1 cut(s) 380
HgaI GACGC 3 cut(s) 178, 204, 1010
HhaI GCGC 3 cut(s) 1382, 1587, 1935
Hin1I GRCGYC 2 cut(s) 328, 1380
Hin6I GCGC 3 cut(s) 1380, 1585, 1933
HinP1I GCGC 3 cut(s) 1380, 1585, 1933
HincII GTYRAC 1 cut(s) 1596
HindII GTYRAC 1 cut(s) 1596
HindIII AAGCTT 4 cut(s) 709, 1829, 2456, 2743
HinfI GANTC 9 cut(s) 388, 467, 812, 909, 1318, 1838, 1883, 2329, 2645
HpaII CCGG 1 cut(s) 380
HphI GGTGA 4 cut(s) 151, 1992, 2229, 2530
Hpy166II GTNNAC 3 cut(s) 1058, 1177, 1596
Hpy188I TCNGA 8 cut(s) 194, 326, 393, 1140, 1452, 1525, 1979, 2334
Hpy8I GTNNAC 3 cut(s) 1058, 1177, 1596
Hpy99I CGWCG 2 cut(s) 632, 1956
HpyCH4III ACNGT 8 cut(s) 565, 656, 673, 897, 1280, 1292, 2232, 2260
HpyCH4IV ACGT 6 cut(s) 176, 328, 687, 936, 1674, 2293
HpyF10VI GCNNNNNNNGC 6 cut(s) 195, 793, 1034, 1124, 1738, 1930
HpyF3I CTNAG 6 cut(s) 1033, 1080, 1244, 1401, 2096, 2694
HpySE526I ACGT 6 cut(s) 176, 328, 687, 936, 1674, 2293
Hsp92I GRCGYC 2 cut(s) 328, 1380
HspAI GCGC 3 cut(s) 1380, 1585, 1933
KasI GGCGCC 1 cut(s) 1379
Kpn2I TCCGGA 1 cut(s) 379
KpnI GGTACC 1 cut(s) 2291
Kzo9I GATC 6 cut(s) 123, 590, 1063, 1997, 2668, 2697
LguI GCTCTTC 2 cut(s) 1223, 1956
LmnI GCTCC 4 cut(s) 106, 1009, 1504, 1857
Lsp1109I GCAGC 3 cut(s) 1032, 1429, 1443
LweI GCATC 6 cut(s) 68, 441, 950, 1242, 1476, 1703
MaeI CTAG 6 cut(s) 426, 1061, 1217, 1625, 1733, 2752
MaeII ACGT 6 cut(s) 176, 328, 687, 936, 1674, 2293
MaeIII GTNAC 3 cut(s) 1670, 2500, 2718
MalI GATC 6 cut(s) 125, 592, 1065, 1999, 2670, 2699
MboI GATC 6 cut(s) 123, 590, 1063, 1997, 2668, 2697
MfeI CAATTG 1 cut(s) 1752
MflI RGATCY 3 cut(s) 123, 1063, 2697
MhlI GDGCHC 2 cut(s) 498, 2237
MlsI TGGCCA 1 cut(s) 41
MluNI TGGCCA 1 cut(s) 41
Mly113I GGCGCC 1 cut(s) 1380
MlyI GAGTC 2 cut(s) 821, 1877
Mox20I TGGCCA 1 cut(s) 41
Mph1103I ATGCAT 1 cut(s) 2416
MroI TCCGGA 1 cut(s) 379
MroXI GAANNNNTTC 4 cut(s) 125, 647, 1157, 2214
MscI TGGCCA 1 cut(s) 41
MslI CAYNNNNRTG 4 cut(s) 246, 657, 1162, 1634
Msp20I TGGCCA 1 cut(s) 41
MspI CCGG 1 cut(s) 380
MunI CAATTG 1 cut(s) 1752
Mva1269I GAATGC 2 cut(s) 303, 1028
MwoI GCNNNNNNNGC 6 cut(s) 195, 793, 1034, 1124, 1738, 1930
NarI GGCGCC 1 cut(s) 1380
NdeI CATATG 1 cut(s) 2416
NdeII GATC 6 cut(s) 123, 590, 1063, 1997, 2668, 2697
NlaIV GGNNCC 5 cut(s) 287, 458, 750, 1381, 2289
NmuCI GTSAC 2 cut(s) 1670, 2718
NsiI ATGCAT 1 cut(s) 2416
NspI RCATGY 3 cut(s) 247, 1438, 1482
NspV TTCGAA 3 cut(s) 1321, 2158, 2254
OliI CACNNNNGTG 1 cut(s) 657
PaeI GCATGC 1 cut(s) 1438
PagI TCATGA 2 cut(s) 262, 2671
PceI AGGCCT 1 cut(s) 1107
PciI ACATGT 1 cut(s) 243
PciSI GCTCTTC 2 cut(s) 1223, 1956
PcsI WCGNNNNNNNCGW 1 cut(s) 693
PctI GAATGC 2 cut(s) 303, 1028
PdmI GAANNNNTTC 4 cut(s) 125, 647, 1157, 2214
PfeI GAWTC 7 cut(s) 388, 467, 909, 1318, 1838, 2329, 2645
PkrI GCNGC 4 cut(s) 1022, 1419, 1433, 1589
PleI GAGTC 2 cut(s) 820, 1877
PluTI GGCGCC 1 cut(s) 1383
PpsI GAGTC 2 cut(s) 820, 1877
Ppu21I YACGTR 1 cut(s) 688
PpuMI RGGWCCY 1 cut(s) 748
PscI ACATGT 1 cut(s) 243
Psp124BI GAGCTC 1 cut(s) 498
Psp5II RGGWCCY 1 cut(s) 748
PspN4I GGNNCC 5 cut(s) 287, 458, 750, 1381, 2289
PspPI GGNCC 2 cut(s) 748, 2140
PspPPI RGGWCCY 1 cut(s) 748
PsrI GAACNNNNNNTAC 2 cut(s) 412, 444
PstI CTGCAG 1 cut(s) 757
PstNI CAGNNNCTG 1 cut(s) 796
PsuI RGATCY 3 cut(s) 123, 1063, 2697
RsaI GTAC 8 cut(s) 430, 824, 960, 1289, 1781, 1899, 2289, 2682
RsaNI GTAC 8 cut(s) 429, 823, 959, 1288, 1780, 1898, 2288, 2681
RseI CAYNNNNRTG 4 cut(s) 246, 657, 1162, 1634
SacI GAGCTC 1 cut(s) 498
SapI GCTCTTC 2 cut(s) 1223, 1956
SatI GCNGC 4 cut(s) 1021, 1418, 1432, 1588
Sau3AI GATC 6 cut(s) 123, 590, 1063, 1997, 2668, 2697
Sau96I GGNCC 2 cut(s) 748, 2140
ScaI AGTACT 1 cut(s) 430
SchI GAGTC 2 cut(s) 821, 1877
SduI GDGCHC 2 cut(s) 498, 2237
SfaNI GCATC 6 cut(s) 68, 441, 950, 1242, 1476, 1703
SfcI CTRYAG 4 cut(s) 753, 788, 1439, 2505
SfoI GGCGCC 1 cut(s) 1381
SfuI TTCGAA 3 cut(s) 1321, 2158, 2254
SinI GGWCC 2 cut(s) 748, 2140
SmiMI CAYNNNNRTG 4 cut(s) 246, 657, 1162, 1634
SmlI CTYRAG 2 cut(s) 358, 2639
SmoI CTYRAG 2 cut(s) 358, 2639
SpeI ACTAGT 1 cut(s) 425
SphI GCATGC 1 cut(s) 1438
SseBI AGGCCT 1 cut(s) 1107
SsiI CCGC 6 cut(s) 349, 454, 588, 1429, 1588, 1855
SspDI GGCGCC 1 cut(s) 1379
SspMI CTAG 6 cut(s) 426, 1061, 1217, 1625, 1733, 2752
SstI GAGCTC 1 cut(s) 498
StuI AGGCCT 1 cut(s) 1107
TaaI ACNGT 8 cut(s) 565, 656, 673, 897, 1280, 1292, 2232, 2260
TaiI ACGT 6 cut(s) 179, 331, 690, 939, 1677, 2296
TaqI TCGA 9 cut(s) 907, 925, 990, 1161, 1316, 1321, 1694, 2158, 2254
TatI WGTACW 3 cut(s) 428, 958, 1287
TauI GCSGC 1 cut(s) 1590
TfiI GAWTC 7 cut(s) 388, 467, 909, 1318, 1838, 2329, 2645
TseFI GTSAC 2 cut(s) 1670, 2718
TseI GCWGC 3 cut(s) 1020, 1417, 1431
Tsp45I GTSAC 2 cut(s) 1670, 2718
TspGWI ACGGA 2 cut(s) 1200, 1569
VpaK11BI GGWCC 2 cut(s) 748, 2140
XapI RAATTY 4 cut(s) 647, 722, 763, 1804
XceI RCATGY 3 cut(s) 247, 1438, 1482
XcmI CCANNNNNNNNNTGG 1 cut(s) 315
XmiI GTMKAC 1 cut(s) 1176
XmnI GAANNNNTTC 4 cut(s) 125, 647, 1157, 2214
XspI CTAG 6 cut(s) 426, 1061, 1217, 1625, 1733, 2752
ZraI GACGTC 1 cut(s) 329
ZrmI AGTACT 1 cut(s) 430
Zsp2I ATGCAT 1 cut(s) 2416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.