Prupe.8G010000_v2.0.a1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000110
Physical Location & Seq
Forward (+)
795926 .. 798681
2756 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G010000.1

Sequence Viewer

Length: 2691 bp
ATGGACTCAGCTCCAACAGCTCTCTTGGTTGGCAAAATTATGGACATTCTCGAGAACGAAGCATACTCCATAGCCGCTGTCCGTGATGAAGTTGATGAGATTAAGCAGGAGCTGGTAAGCATGACATCCTTCCTAGAGGATGTTGAGGGCAAGAAAACACAGACAGAAACACAGAAAGCTTGGGTCACAAGTGTCAGAGACTTAACCAGTGATGTTGAAGATATCATCGATGAATTCATGTATCATACGTATGAGCAGCAAAGTAGGGGACGATTTGCAAGGTGGCTCCACAGGACCATTCATATTCCAAAGAATCTCTTCTATCGGCGTAAAATTGCCAACAAGTTACACAAAATCACTAAAATGATCAAGGCCATTCCAGAGAGAAATAAGAGATATGCTCTTGATGGTGTAGTAGGAACAAGTTGGGATGATATTAGCAAATGGGTCAAGAACCAAGCAGTGTCTTCTCTCTTTATTAACAAAGATGAACTCGTCGGGATTGATGGAAAGAAGCAAACATTAACTGCATGGCTGCTCAATGAAGAGCAACACCTAACTGTTGTCTCCGTGGTCGGGATGGGAGGATCCGGGAAAACAACTCTAGTTGCCAAAACCTTCGCCAACGAAACTGTAAAGAGACATTTTGATTCTTATGCTTGGATCACTGTCTCCCAAACTTATGTGATTGAAGACTTGTTTCGGAGCTTGATCAAGGAACTCCACCAAACAAGGAATGAATATGTCCCTGCAGACCTGATATCCATGGGCTACAGAGACTTGATACAACTTATGCTGAACTACCTGGAGTCTAAAAGGTACCTAGTAGTACTGGATGACGTGTGGGATATCAAACTTTGGAGAGAAATAAGGATATCACTTCCAGATAGACAGCTTGGAAGTCGAATCATGTTTACAACTCGAAAAGAAGACATAGCATTCCATTGTTTTGGAGTTGAAAGCCATGTTCACTGTATGCAACCCCTGGAAAAGAATTACGCTTGGGAGCTTTTCAGCAGGAAATCATTCTCTACCTTTGATGGTAAATGTTGTCCACCAGAGCTTGAAAAATTAGCTTGGGAACTCATGGAAAAGTGTAAAGGCCTACCTCTAGCTATCATAGCTTTAGGTGGTCTTATGTCTTCCAAGAAATTAGCTGCGGAGTGGAGTAAAGTCTACAATGGCTTAAATTGGCATCTAACAAGCCATCATTTGTTAGAACCTGTGAAGAGCATCTTGTTACTTAGTTTCAATGATTTGCCATATCGCTTGAAGCATTGTTTTCTATATTGTTCCCTTTTTCCAGAAGATTATTTGATTCGAAGGAAAAGGCTCATTAGGTTATGGATAGCTGAAGGATTTGTTGAACATGCTAGAGGGGTCACACCTGAACAAGTTGCAGATAGCTATCTTATGGAACTCATTTTTCGTAACATGCTACAAGTTGTAGAGAGGAATGAAACCGGTAGGCCAAAATCATGTAAGATGCATATGACCTTATGCGAGAGCTTGCTCTGTCAACATCTGAGAAAGAAAAATTTTCTATTGTACATGATGGGAAAGAAGTACTGGAAGACATTGGAGCCCGCCGTTTGTCAATCCAAACAACCCAAGGAGGAATTGAATCTTGCATTGTCATTGCCTTCTGGATTCAAATTGTTGAGGGTTTTAGATTTGGAGGATGTCCAAATTGATAAGCTGCCACATAATTTAGTGTACTTATTCAACTTAAGATACTTAAGTTTGAAGGGAACTCAAATTAAGGAGCTTCCAAAAGCCATAGGACTGCTCCGCAACCTTCAGACATTGAACATCCTCAATACTAAGATAGAGGTACTTCCCAGAGGTATTTCCAAGTTGCAAAACCTGCGCCATCTAATTATGCTCCGTCATTCTGGAGAGTATATGGCCTTCAAAACTGCCCATGGGACAAGAGTGCCATTTAATATTAGTAAGCTGAAGAAATTGGAAGTTTTGTCATGTGTTGAATCAGAAGGAAACATTATAAGGCTCATTGGGAATATGACTCAACTTACAAGGATTGGCATTACGAATGTAAAAGAGAGAGACGCAATGGACCTATGTGATTCAATTCAAAAGTTGAAGCTACTTCAGTGCTTGGCTTTAAGGGTAAGTGGTGAGGAGGAGTTTCTTGACGTCAATGCATTATCTTCACCTCCTCCACACCTTCGAAAACTTATTTTTGGCAGCAAGCTGCAAAAGGTACCACCTTGGTTCTCTTCGCTCCAGAACCTTACATATCTGTATCTTCATTGGACGAGACTTGACGAGGATTTACTACCTCATATTGAAGCATTGCCATGTCTGGGAAGGCTTTTACTTGTCAATGCCTATGTTGGTAATGAGTTGTGTTTCAACAGAGGCTTTCCAAAGCTTACTATTCTGGAGTTGTTTAATTTCCCCTTATTGAATAAGATAACTATAGCAGAAGGGGTGATGAGGAATCTCCGGTTATTAACACTTGCTAGATGTATGGAGTTAAAGGCATTGCCACAGGGTTTTGAATACCTTAGTAAGCTAGAAACACTTGAATTGTTGTCTGTTTCAATGCAACTTATAGAGTCCATACAAGAAGGAGGGGTGGATCATCCAACGGTAAAACACATCACCGTCATTACAAATTATTCTTTGAAATGCTTGATAAGAGCTCATCATAGTTCATCCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

897

Amino Acids

103.39

Weight (kDa)

8.94

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2006
AatII GACGTC 1 cut(s) 2160
Acc36I ACCTGC 1 cut(s) 1875
Acc65I GGTACC 2 cut(s) 819, 2224
AccB1I GGYRCC 2 cut(s) 819, 2224
AccI GTMKAC 1 cut(s) 1176
AciI CCGC 4 cut(s) 75, 1160, 1587, 1792
AclWI GGATC 4 cut(s) 582, 595, 671, 2613
AcsI RAATTY 2 cut(s) 233, 1537
AcuI CTGAAG 4 cut(s) 1374, 1784, 1979, 2096
AcyI GRCGYC 1 cut(s) 2157
AfaI GTAC 7 cut(s) 821, 831, 1550, 1568, 1718, 1836, 2226
AfiI CCNNNNNNNGG 2 cut(s) 576, 2327
AflII CTTAAG 2 cut(s) 1729, 1738
AflIII ACRYGT 1 cut(s) 840
AgeI ACCGGT 1 cut(s) 1463
AjiI CACGTC 1 cut(s) 841
AjnI CCWGG 2 cut(s) 804, 984
Alw21I GWGCWC 1 cut(s) 2671
Alw26I GTCTC 7 cut(s) 192, 571, 634, 676, 771, 2061, 2275
AlwI GGATC 4 cut(s) 582, 595, 671, 2613
AlwNI CAGNNNCTG 1 cut(s) 112
Ama87I CYCGRG 1 cut(s) 50
AoxI GGCC 4 cut(s) 372, 1102, 1469, 1908
ApeKI GCWGC 6 cut(s) 256, 535, 1157, 1699, 2208, 2215
ApoI RAATTY 2 cut(s) 233, 1537
AsiGI ACCGGT 1 cut(s) 1463
Asp700I GAANNNNTTC 2 cut(s) 317, 1025
Asp718I GGTACC 2 cut(s) 819, 2224
AspLEI GCGC 1 cut(s) 1872
AspS9I GGNCC 2 cut(s) 294, 2077
AsuC2I CCSGG 1 cut(s) 592
AsuHPI GGTGA 4 cut(s) 2150, 2166, 2467, 2620
AsuII TTCGAA 2 cut(s) 1321, 2191
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 2 cut(s) 294, 2077
BaeI ACNNNNGTAYC 2 cut(s) 1726, 1759
BamHI GGATCC 1 cut(s) 587
BanI GGYRCC 2 cut(s) 819, 2224
BanII GRGCYC 2 cut(s) 1587, 2671
BarI GAAGNNNNNNTAC 2 cut(s) 1895, 1927
BbsI GAAGAC 5 cut(s) 459, 699, 936, 1134, 1580
Bbv12I GWGCWC 1 cut(s) 2671
BbvI GCAGC 6 cut(s) 268, 522, 1144, 1686, 2202, 2220
BccI CCATC 7 cut(s) 401, 500, 574, 1034, 1215, 1549, 1881
BceAI ACGGC 1 cut(s) 1574
BciT130I CCWGG 2 cut(s) 806, 986
BclI TGATCA 2 cut(s) 366, 711
BcnI CCSGG 1 cut(s) 592
BcoDI GTCTC 7 cut(s) 192, 571, 634, 676, 771, 2061, 2275
BfaI CTAG 7 cut(s) 134, 605, 824, 1112, 1374, 2487, 2540
BfmI CTRYAG 3 cut(s) 750, 772, 2442
BfrI CTTAAG 2 cut(s) 1729, 1738
BfuAI ACCTGC 1 cut(s) 1875
BisI GCNGC 7 cut(s) 75, 257, 536, 1158, 1700, 2209, 2216
BlsI GCNGC 7 cut(s) 76, 258, 537, 1159, 1701, 2210, 2217
BmcAI AGTACT 2 cut(s) 831, 1568
Bme1390I CCNGG 3 cut(s) 592, 806, 986
Bme18I GGWCC 2 cut(s) 294, 2077
BmeT110I CYCGRG 1 cut(s) 50
BmgBI CACGTC 1 cut(s) 841
BmgT120I GGNCC 2 cut(s) 294, 2077
BmiI GGNNCC 5 cut(s) 287, 589, 821, 1584, 2226
BmrFI CCNGG 3 cut(s) 592, 806, 986
BmsI GCATC 3 cut(s) 1204, 1242, 1476
BpiI GAAGAC 5 cut(s) 459, 699, 936, 1134, 1580
BplI GAGNNNNNCTC 2 cut(s) 385, 417
BpmI CTGGAG 4 cut(s) 827, 1917, 2231, 2426
Bpu14I TTCGAA 2 cut(s) 1321, 2191
BpuMI CCSGG 1 cut(s) 592
Bsa29I ATCGAT 1 cut(s) 228
BsaAI YACGTR 1 cut(s) 249
BsaBI GATNNNNATC 1 cut(s) 1407
BsaHI GRCGYC 1 cut(s) 2157
BsaJI CCNNGG 6 cut(s) 570, 765, 984, 1611, 1924, 2231
BsaWI WCCGGW 2 cut(s) 1463, 2469
Bsc4I CCNNNNNNNGG 2 cut(s) 576, 2327
Bse118I RCCGGY 1 cut(s) 1463
Bse1I ACTGG 3 cut(s) 207, 837, 1574
Bse3DI GCAATG 4 cut(s) 1637, 2079, 2315, 2507
Bse8I GATNNNNATC 1 cut(s) 1407
BseBI CCWGG 2 cut(s) 806, 986
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 6 cut(s) 570, 765, 984, 1611, 1924, 2231
BseGI GGATG 9 cut(s) 125, 145, 436, 585, 841, 1687, 1812, 2608, 2681
BseJI GATNNNNATC 1 cut(s) 1407
BseLI CCNNNNNNNGG 2 cut(s) 576, 2327
BseMI GCAATG 4 cut(s) 1637, 2079, 2315, 2507
BseMII CTCAG 2 cut(s) 21, 1517
BseNI ACTGG 3 cut(s) 207, 837, 1574
BseRI GAGGAG 3 cut(s) 2156, 2159, 2169
BseXI GCAGC 6 cut(s) 268, 522, 1144, 1686, 2202, 2220
BshFI GGCC 4 cut(s) 374, 1104, 1471, 1910
BshNI GGYRCC 2 cut(s) 819, 2224
BshTI ACCGGT 1 cut(s) 1463
BshVI ATCGAT 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 2671
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 3 cut(s) 591, 1464, 2470
BslFI GGGAC 3 cut(s) 282, 731, 1942
BslI CCNNNNNNNGG 2 cut(s) 576, 2327
BsmAI GTCTC 7 cut(s) 192, 571, 634, 676, 771, 2061, 2275
BsmBI CGTCTC 1 cut(s) 2061
BsmFI GGGAC 3 cut(s) 282, 731, 1942
BsmI GAATGC 1 cut(s) 938
BsnI GGCC 4 cut(s) 374, 1104, 1471, 1910
BsoBI CYCGRG 1 cut(s) 50
Bsp119I TTCGAA 2 cut(s) 1321, 2191
Bsp1286I GDGCHC 2 cut(s) 1587, 2671
Bsp1407I TGTACA 1 cut(s) 1548
Bsp143I GATC 5 cut(s) 366, 587, 663, 711, 2605
Bsp19I CCATGG 2 cut(s) 765, 1924
BspACI CCGC 4 cut(s) 75, 1160, 1587, 1792
BspANI GGCC 4 cut(s) 374, 1104, 1471, 1910
BspCNI CTCAG 2 cut(s) 20, 1518
BspDI ATCGAT 1 cut(s) 228
BspLI GGNNCC 5 cut(s) 287, 589, 821, 1584, 2226
BspMAI CTGCAG 1 cut(s) 754
BspMI ACCTGC 1 cut(s) 1875
BspPI GGATC 4 cut(s) 582, 595, 671, 2613
BspQI GCTCTTC 2 cut(s) 540, 1223
BspT104I TTCGAA 2 cut(s) 1321, 2191
BspT107I GGYRCC 2 cut(s) 819, 2224
BspTI CTTAAG 2 cut(s) 1729, 1738
BsrDI GCAATG 4 cut(s) 1637, 2079, 2315, 2507
BsrFI RCCGGY 1 cut(s) 1463
BsrGI TGTACA 1 cut(s) 1548
BsrI ACTGG 3 cut(s) 207, 837, 1574
BssAI RCCGGY 1 cut(s) 1463
BssECI CCNNGG 6 cut(s) 570, 765, 984, 1611, 1924, 2231
BssMI GATC 5 cut(s) 366, 587, 663, 711, 2605
BssNI GRCGYC 1 cut(s) 2157
BssT1I CCWWGG 4 cut(s) 765, 1611, 1924, 2231
Bst2UI CCWGG 2 cut(s) 806, 986
Bst4CI ACNGT 6 cut(s) 562, 634, 670, 974, 2617, 2632
Bst6I CTCTTC 4 cut(s) 323, 540, 1223, 2245
BstACI GRCGYC 1 cut(s) 2157
BstAFI CTTAAG 2 cut(s) 1729, 1738
BstAPI GCANNNNNTGC 1 cut(s) 1867
BstAUI TGTACA 1 cut(s) 1548
BstBAI YACGTR 1 cut(s) 249
BstBI TTCGAA 2 cut(s) 1321, 2191
BstC8I GCNNGC 3 cut(s) 1511, 1587, 2213
BstDEI CTNAG 5 cut(s) 7, 1244, 1526, 1824, 2531
BstDSI CCRYGG 3 cut(s) 570, 765, 1924
BstF5I GGATG 9 cut(s) 125, 145, 436, 585, 841, 1687, 1812, 2608, 2681
BstHHI GCGC 1 cut(s) 1872
BstKTI GATC 5 cut(s) 369, 590, 666, 714, 2608
BstMAI GTCTC 7 cut(s) 192, 571, 634, 676, 771, 2061, 2275
BstMBI GATC 5 cut(s) 366, 587, 663, 711, 2605
BstMWI GCNNNNNNNGC 3 cut(s) 17, 1121, 1867
BstNI CCWGG 2 cut(s) 806, 986
BstNSI RCATGY 2 cut(s) 1373, 1438
BstSCI CCNGG 3 cut(s) 590, 804, 984
BstSFI CTRYAG 3 cut(s) 750, 772, 2442
BstSNI TACGTA 1 cut(s) 249
BstV1I GCAGC 6 cut(s) 268, 522, 1144, 1686, 2202, 2220
BstV2I GAAGAC 5 cut(s) 459, 699, 936, 1134, 1580
BstX2I RGATCY 1 cut(s) 587
BstXI CCANNNNNNTGG 1 cut(s) 950
BstYI RGATCY 1 cut(s) 587
Bsu15I ATCGAT 1 cut(s) 228
BsuRI GGCC 4 cut(s) 374, 1104, 1471, 1910
BsuTUI ATCGAT 1 cut(s) 228
BtgI CCRYGG 3 cut(s) 570, 765, 1924
BtrI CACGTC 1 cut(s) 841
BtsCI GGATG 9 cut(s) 125, 145, 436, 585, 841, 1687, 1812, 2608, 2681
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 5 cut(s) 214, 468, 666, 970, 2120
BveI ACCTGC 1 cut(s) 1875
Cac8I GCNNGC 3 cut(s) 1511, 1587, 2213
CaiI CAGNNNCTG 1 cut(s) 112
CfoI GCGC 1 cut(s) 1872
Cfr10I RCCGGY 1 cut(s) 1463
Cfr13I GGNCC 2 cut(s) 294, 2077
ClaI ATCGAT 1 cut(s) 228
CseI GACGC 1 cut(s) 2078
Csp6I GTAC 7 cut(s) 820, 830, 1549, 1567, 1717, 1835, 2225
CspAI ACCGGT 1 cut(s) 1463
CviQI GTAC 7 cut(s) 820, 830, 1549, 1567, 1717, 1835, 2225
DdeI CTNAG 5 cut(s) 7, 1244, 1526, 1824, 2531
DpnI GATC 5 cut(s) 368, 589, 665, 713, 2607
DpnII GATC 5 cut(s) 366, 587, 663, 711, 2605
Eam1104I CTCTTC 4 cut(s) 323, 540, 1223, 2245
EarI CTCTTC 4 cut(s) 323, 540, 1223, 2245
Ecl136II GAGCTC 1 cut(s) 2669
Eco105I TACGTA 1 cut(s) 249
Eco130I CCWWGG 4 cut(s) 765, 1611, 1924, 2231
Eco147I AGGCCT 1 cut(s) 1104
Eco24I GRGCYC 2 cut(s) 1587, 2671
Eco32I GATATC 4 cut(s) 223, 762, 850, 876
Eco47I GGWCC 2 cut(s) 294, 2077
Eco53kI GAGCTC 1 cut(s) 2669
Eco57I CTGAAG 4 cut(s) 1374, 1784, 1979, 2096
Eco88I CYCGRG 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 2669
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 2 cut(s) 804, 984
EcoRV GATATC 4 cut(s) 223, 762, 850, 876
EcoT14I CCWWGG 4 cut(s) 765, 1611, 1924, 2231
EcoT22I ATGCAT 2 cut(s) 1491, 2167
EcoT38I GRGCYC 2 cut(s) 1587, 2671
ErhI CCWWGG 4 cut(s) 765, 1611, 1924, 2231
Esp3I CGTCTC 1 cut(s) 2061
FalI AAGNNNNNCTT 4 cut(s) 302, 334, 1220, 1252
FaqI GGGAC 3 cut(s) 282, 731, 1942
FauI CCCGC 1 cut(s) 1594
FauNDI CATATG 1 cut(s) 1491
FbaI TGATCA 2 cut(s) 366, 711
FblI GTMKAC 1 cut(s) 1176
Fnu4HI GCNGC 7 cut(s) 75, 257, 536, 1158, 1700, 2209, 2216
FokI GGATG 9 cut(s) 112, 152, 443, 592, 848, 1694, 1799, 2595, 2668
FriOI GRGCYC 2 cut(s) 1587, 2671
Fsp4HI GCNGC 7 cut(s) 75, 257, 536, 1158, 1700, 2209, 2216
FspBI CTAG 7 cut(s) 134, 605, 824, 1112, 1374, 2487, 2540
GlaI GCGC 1 cut(s) 1871
GluI GCNGC 7 cut(s) 75, 257, 536, 1158, 1700, 2209, 2216
GsuI CTGGAG 4 cut(s) 827, 1917, 2231, 2426
HaeIII GGCC 4 cut(s) 374, 1104, 1471, 1910
HapII CCGG 3 cut(s) 591, 1464, 2470
HgaI GACGC 1 cut(s) 2078
HhaI GCGC 1 cut(s) 1872
Hin1I GRCGYC 1 cut(s) 2157
Hin6I GCGC 1 cut(s) 1870
HinP1I GCGC 1 cut(s) 1870
HincII GTYRAC 1 cut(s) 1520
HindII GTYRAC 1 cut(s) 1520
HindIII AAGCTT 2 cut(s) 177, 2393
HpaII CCGG 3 cut(s) 591, 1464, 2470
HphI GGTGA 4 cut(s) 2150, 2166, 2467, 2620
Hpy166II GTNNAC 6 cut(s) 915, 970, 1055, 1177, 1520, 1717
Hpy188I TCNGA 5 cut(s) 197, 705, 1527, 1803, 1993
Hpy8I GTNNAC 6 cut(s) 915, 970, 1055, 1177, 1520, 1717
Hpy99I CGWCG 1 cut(s) 500
HpyCH4III ACNGT 6 cut(s) 562, 634, 670, 974, 2617, 2632
HpyCH4IV ACGT 3 cut(s) 248, 840, 2157
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 1121, 1867
HpyF3I CTNAG 5 cut(s) 7, 1244, 1526, 1824, 2531
HpySE526I ACGT 3 cut(s) 248, 840, 2157
Hsp92I GRCGYC 1 cut(s) 2157
HspAI GCGC 1 cut(s) 1870
KpnI GGTACC 2 cut(s) 823, 2228
Ksp22I TGATCA 2 cut(s) 366, 711
Kzo9I GATC 5 cut(s) 366, 587, 663, 711, 2605
LguI GCTCTTC 2 cut(s) 540, 1223
Lsp1109I GCAGC 6 cut(s) 268, 522, 1144, 1686, 2202, 2220
LweI GCATC 3 cut(s) 1204, 1242, 1476
MaeI CTAG 7 cut(s) 134, 605, 824, 1112, 1374, 2487, 2540
MaeII ACGT 3 cut(s) 248, 840, 2157
MaeIII GTNAC 5 cut(s) 184, 345, 1239, 1381, 1430
MalI GATC 5 cut(s) 368, 589, 665, 713, 2607
MboI GATC 5 cut(s) 366, 587, 663, 711, 2605
MflI RGATCY 1 cut(s) 587
MhlI GDGCHC 2 cut(s) 1587, 2671
MlyI GAGTC 3 cut(s) 818, 2020, 2591
MmeI TCCRAC 2 cut(s) 38, 2636
Mph1103I ATGCAT 2 cut(s) 1491, 2167
MroXI GAANNNNTTC 2 cut(s) 317, 1025
MslI CAYNNNNRTG 4 cut(s) 249, 362, 2320, 2686
MspA1I CMGCKG 1 cut(s) 77
MspCI CTTAAG 2 cut(s) 1729, 1738
MspI CCGG 3 cut(s) 591, 1464, 2470
MspR9I CCNGG 3 cut(s) 592, 806, 986
Mva1269I GAATGC 1 cut(s) 938
MvaI CCWGG 2 cut(s) 806, 986
MwoI GCNNNNNNNGC 3 cut(s) 17, 1121, 1867
NciI CCSGG 1 cut(s) 592
NcoI CCATGG 2 cut(s) 765, 1924
NdeI CATATG 1 cut(s) 1491
NdeII GATC 5 cut(s) 366, 587, 663, 711, 2605
NlaIV GGNNCC 5 cut(s) 287, 589, 821, 1584, 2226
NmuCI GTSAC 2 cut(s) 184, 1381
NsiI ATGCAT 2 cut(s) 1491, 2167
NspI RCATGY 2 cut(s) 1373, 1438
NspV TTCGAA 2 cut(s) 1321, 2191
PaeR7I CTCGAG 1 cut(s) 50
PceI AGGCCT 1 cut(s) 1104
PciSI GCTCTTC 2 cut(s) 540, 1223
PctI GAATGC 1 cut(s) 938
PdmI GAANNNNTTC 2 cut(s) 317, 1025
PfeI GAWTC 9 cut(s) 313, 650, 906, 1318, 1624, 1650, 1988, 2087, 2464
PfoI TCCNGGA 1 cut(s) 590
PinAI ACCGGT 1 cut(s) 1463
PkrI GCNGC 7 cut(s) 76, 258, 537, 1159, 1701, 2210, 2217
PleI GAGTC 3 cut(s) 817, 2020, 2590
PpsI GAGTC 3 cut(s) 817, 2020, 2590
Ppu21I YACGTR 1 cut(s) 249
PsiI TTATAA 1 cut(s) 2006
Psp124BI GAGCTC 1 cut(s) 2671
Psp6I CCWGG 2 cut(s) 804, 984
PspGI CCWGG 2 cut(s) 804, 984
PspN4I GGNNCC 5 cut(s) 287, 589, 821, 1584, 2226
PspPI GGNCC 2 cut(s) 294, 2077
PsrI GAACNNNNNNTAC 2 cut(s) 47, 79
PstI CTGCAG 1 cut(s) 754
PstNI CAGNNNCTG 1 cut(s) 112
PsuI RGATCY 1 cut(s) 587
RsaI GTAC 7 cut(s) 821, 831, 1550, 1568, 1718, 1836, 2226
RsaNI GTAC 7 cut(s) 820, 830, 1549, 1567, 1717, 1835, 2225
RseI CAYNNNNRTG 4 cut(s) 249, 362, 2320, 2686
SacI GAGCTC 1 cut(s) 2671
SapI GCTCTTC 2 cut(s) 540, 1223
SatI GCNGC 7 cut(s) 75, 257, 536, 1158, 1700, 2209, 2216
Sau3AI GATC 5 cut(s) 366, 587, 663, 711, 2605
Sau96I GGNCC 2 cut(s) 294, 2077
ScaI AGTACT 2 cut(s) 831, 1568
SchI GAGTC 3 cut(s) 818, 2020, 2591
ScrFI CCNGG 3 cut(s) 592, 806, 986
SduI GDGCHC 2 cut(s) 1587, 2671
SfaNI GCATC 3 cut(s) 1204, 1242, 1476
SfcI CTRYAG 3 cut(s) 750, 772, 2442
Sfr274I CTCGAG 1 cut(s) 50
SfuI TTCGAA 2 cut(s) 1321, 2191
SinI GGWCC 2 cut(s) 294, 2077
SlaI CTCGAG 1 cut(s) 50
SmiMI CAYNNNNRTG 4 cut(s) 249, 362, 2320, 2686
SmlI CTYRAG 3 cut(s) 50, 1729, 1738
SmoI CTYRAG 3 cut(s) 50, 1729, 1738
SnaBI TACGTA 1 cut(s) 249
SseBI AGGCCT 1 cut(s) 1104
SsiI CCGC 4 cut(s) 75, 1160, 1587, 1792
SspI AATATT 1 cut(s) 1948
SspMI CTAG 7 cut(s) 134, 605, 824, 1112, 1374, 2487, 2540
SstI GAGCTC 1 cut(s) 2671
StuI AGGCCT 1 cut(s) 1104
StyD4I CCNGG 3 cut(s) 590, 804, 984
StyI CCWWGG 4 cut(s) 765, 1611, 1924, 2231
TaaI ACNGT 6 cut(s) 562, 634, 670, 974, 2617, 2632
TaiI ACGT 3 cut(s) 251, 843, 2160
TaqI TCGA 6 cut(s) 51, 228, 904, 922, 1321, 2191
TatI WGTACW 4 cut(s) 829, 1548, 1566, 1716
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 9 cut(s) 313, 650, 906, 1318, 1624, 1650, 1988, 2087, 2464
TscAI CASTG 5 cut(s) 214, 468, 673, 977, 2120
TseFI GTSAC 2 cut(s) 184, 1381
TseI GCWGC 6 cut(s) 256, 535, 1157, 1699, 2208, 2215
Tsp45I GTSAC 2 cut(s) 184, 1381
TspGWI ACGGA 3 cut(s) 71, 559, 1877
TspRI CASTG 5 cut(s) 214, 468, 673, 977, 2120
Vha464I CTTAAG 2 cut(s) 1729, 1738
VpaK11BI GGWCC 2 cut(s) 294, 2077
XapI RAATTY 2 cut(s) 233, 1537
XceI RCATGY 2 cut(s) 1373, 1438
XhoI CTCGAG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 1176
XmnI GAANNNNTTC 2 cut(s) 317, 1025
XspI CTAG 7 cut(s) 134, 605, 824, 1112, 1374, 2487, 2540
ZraI GACGTC 1 cut(s) 2158
ZrmI AGTACT 2 cut(s) 831, 1568
Zsp2I ATGCAT 2 cut(s) 1491, 2167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.