Rroxscaffold_1G00059340

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000110
Physical Location & Seq
Reverse (-)
81352545 .. 81358054
5510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059340.1

Sequence Viewer

Length: 2754 bp
ATGGCCTCATGTGTAACAGACCTCTTAATTAAAAAAATTGTGGCAATTCTTGAGAATGAAGGAACAACCATATCCAGTGTTCGTGATGAAGTTGATAGCATTAAGGAGGAGCTTCAAAGCATGAAGGCTTTCCTAGTGGATGCAGAGGCCGACACAGCAAAAACTGAAGGAGAGAAATTGTGGGTTGAAAGCATCAGAAACTTGGCCTATGATGTTGAAGATATCATTGATAAATTCATGTATCACATGTATGAGAAGCAAAGTGGGAGTCGACTTTCAAGGAGGCTACACAAAACCATTTGCTTTCCAAAAAGTCTTTGGTTTAGACGTAAAACTGCAAAGAGGTTACAGAAAATCACTAGAAAGATCAAAGACATTCCAGAGAGGAATCAAAGGTACGGTGTTCGCCTTGTAGCAGGAGCAACTACATCTGAAGATATTCACAAATTGGTGCAGAACCAAGCCGAGTCTTCGTTTTTCATTATGGAAGACGAACTGGTTGGGATTGAAGGCAAGAAGCAAATACTAATGGGATGGCTCATGGATGAAGAGCAACACCAAACTGTTATATCTGTGGTTGGCATGGGAGGTTCTGGAAAGACAACTCTAGTTGCCAAGACCTTCAACAACGAAAAGGTGAAGAGACATTTCGATTGTTATGCATGGATTACCGTTTCCCAAACTTATGATGTGGTACACTTGTGTGGAAGCTTGATCAGGGAACTCTACAAATCAAGGAAGGAAGATTTCCCTGCAAATTTGAATGCGATGAGCCGCAAAGAATTGCTAGAGTTACTAGTTAATTACTTGGAGTCCAATAAATACCTGGTTGTTCTAGATGACGTGTGGGATATAAAAGTTTGGAGAGAAATAAAGGTATCACTTCAAGAAAGGCAACTCGGAAGTCGAATCATAGTTACAACTCGAAAAGAAGATGTAGCATCCAATTCTTTTGGAGTTAGAAGCCATGTTCACCATATTCAACCCCTGCTGAAGAATGAGGCTTGGGAGCTCTTCTGCAAAAAAGCATTCTCATCTAATGAGCACAGAACTTGTCCACCTCATTTTGTGTCATTAGCATGCCAACTTGTAGAGAAGTGTGAAGGCCTTCCTCTGGCTATTGTGGCTTTAGGGGGTCTTATGTCTTCCAAGAAGTCGCTGGATCAATGGCAGCAAGTCTGCAACAGCCTGAATTGGCATCTAGATAACAATTCATTGCTAGACACTGTGAAAAACATCTTGTTGCTGAGTTTCAATGATTTGCCATCTCAATTGAAACATTGCTTCCTCTATTGCTCTCTATTCCCAGAAGATTTTGTAATTAGAAGAAAAAGGCTCATTAGATTGTGGATAGCTGAAGGATTTGTCGAACATGTGAAAGGGATCACCCCCGAAGAAGTTGGAGATGGCTATCTTTGGGAACTCTGCTTCCGTAGCATGTTACAAGTTGTAAAGAGATATGGAACAGGAAGGCCGAGAAAAGTTAAGATGCATGATCTGATGCGAGAGCTTGCTTTGTCGACAGCCGAGAAGGAAAAGTTTGGTCTTATATACGACGGGAGAGAAGGAATGGAAGACATCTCAACCCGTCGCTTATCAATTCACAAAATGGAAGGAGAAATTAAATCATGGCCGGGTATGTCAAAGATTCGTTCTCTTCTTGTATTTGCAACTGACATGTCCTCATCGTCTTTCTCAAATGCACTGGTTTCTCAGTTCAAATTGTTGAGGACTCTAGACTTGGAGGATGTCCAAATTGATAAACTGCCAGATGCAGTTGTTTATATGTTCAACTTGAGATACTTAAATTTGAAGGGCACTTTAATCGAGGAACTTCCAGAGTCCATAGGGCGTCTCCGCAACCTTCAATTTTTGAAAATCGAGGAAACCAAGATAGAGGCACTTCCAGAAGGAATTGCAAAATTGTTGAACCTGCGCCATCTAAGTATGTTTCGGTTGACTGGAACCACTCTGGACTTCAAACTTTATAGTGGGACAAAAGCGCCATCAAATATTTGTCAGTTGCAGAAATTGCAGAGTTTAAAAACGACTGAATCAGAAGGGGACACTATTAGCCTTATCGGCAACATGACTCAACTCAGGGTCATCGGCATATCGAATGTGAAAGAAAGAGATGAGAATGACCTATGGGTTTCAATTAAGAAGCTCACTCTGCTTCAATCTTTGGTTTTAATGGCAAGTAATGAAGATGAAATCCTTCCGGTCAAAGCACAATGTCCACCTCTTCTGCACCTTCGAAAGCTTAAATTGATTGGCAGACTGCAAAACGTAGTACCTTGGTTTTCTTCACTGCATAACCTCACATCTCTGTATCTGCATTGGTCAAGACTTGAAGACGATGTACTACCTCAAATTGAAGCACTGCCCAATCTGACAAGGCTTCGTCTTTGTAATGCATATGTTGGGGAAGAGTTGTGTTTCCGCAGAGGCTTTGTAAAGCTGGAGAGGTTGTATTTGTTGAATTTTGCCTCGGTGAAAAAGATTACTATAGAAAAAGGAGTGATGCCAAATCTCCACAACTTATGGGTTGAACGGTGCATGGAATTGAAGACAATGCCACTAGGCCTTGAATACCTTTCTAATCTACAATCTATGGGCTTGGCATTTCTTCCCGTTGAAGTTGTAAAGCCCATTCGGAAAGGAGGTATGGATCGGTCTAAGGTACAACACATCCCCGAGATCTTACATGTTTTCGAACAGTCATCCAAGCAGGTCTACGAAAACTTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

917

Amino Acids

105.42

Weight (kDa)

8.75

Isoelectric Point (pI)

51.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 93 1.3e-21 Rx N-terminal domain
NB-ARC PF00931 172 - 347 1.3e-46 NB-ARC domain
WHD_DRP PF23559 434 - 505 2.1e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 549 - 872 5e-42 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1941, 2722
AccI GTMKAC 3 cut(s) 271, 1520, 2736
AciI CCGC 3 cut(s) 775, 1858, 2443
AclWI GGATC 3 cut(s) 1170, 1391, 2679
AcoI YGGCCR 1 cut(s) 1631
AcsI RAATTY 4 cut(s) 233, 757, 1807, 2482
AcuI CTGAAG 4 cut(s) 186, 453, 1013, 1377
AcyI GRCGYC 1 cut(s) 1852
AfaI GTAC 5 cut(s) 398, 696, 2295, 2364, 2685
AfiI CCNNNNNNNGG 1 cut(s) 1114
AflIII ACRYGT 5 cut(s) 246, 843, 1372, 1677, 2707
AhlI ACTAGT 1 cut(s) 796
AjiI CACGTC 1 cut(s) 844
AjnI CCWGG 1 cut(s) 825
AleI CACNNNNGTG 1 cut(s) 702
AloI GAACNNNNNNTCC 2 cut(s) 1413, 1445
AluBI AGCT 8 cut(s) 112, 711, 1012, 1355, 1510, 2167, 2263, 2461
AluI AGCT 8 cut(s) 112, 711, 1012, 1355, 1510, 2167, 2263, 2461
Alw21I GWGCWC 2 cut(s) 1014, 1047
Alw26I GTCTC 2 cut(s) 637, 1859
AlwI GGATC 3 cut(s) 1170, 1391, 2679
Ama87I CYCGRG 1 cut(s) 2696
AoxI GGCC 8 cut(s) 3, 147, 204, 1105, 1472, 1631, 2584, 2748
ApeKI GCWGC 1 cut(s) 1171
ApoI RAATTY 4 cut(s) 233, 757, 1807, 2482
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 4 cut(s) 128, 438, 1107, 2217
AspLEI GCGC 2 cut(s) 1938, 2005
AsuC2I CCSGG 1 cut(s) 1635
AsuHPI GGTGA 4 cut(s) 649, 965, 1378, 2506
AsuII TTCGAA 2 cut(s) 2257, 2715
AvaI CYCGRG 1 cut(s) 2696
BaeGI GKGCMC 1 cut(s) 1820
BaeI ACNNNNGTAYC 2 cut(s) 686, 719
BanII GRGCYC 1 cut(s) 1014
BarI GAAGNNNNNNTAC 2 cut(s) 2346, 2378
BbsI GAAGAC 6 cut(s) 462, 495, 1137, 1581, 2361, 2576
Bbv12I GWGCWC 2 cut(s) 1014, 1047
BbvI GCAGC 1 cut(s) 1183
BccI CCATC 5 cut(s) 528, 1273, 1400, 1947, 2014
BcgI CGANNNNNNTGC 4 cut(s) 641, 675, 1807, 1841
BciT130I CCWGG 1 cut(s) 827
BclI TGATCA 1 cut(s) 714
BcnI CCSGG 1 cut(s) 1635
BcoDI GTCTC 2 cut(s) 637, 1859
BcuI ACTAGT 1 cut(s) 796
BfmI CTRYAG 1 cut(s) 2508
BfoI RGCGCY 1 cut(s) 2006
BfuAI ACCTGC 2 cut(s) 1941, 2722
BglI GCCNNNNNGGC 1 cut(s) 2082
BglII AGATCT 1 cut(s) 2700
BisI GCNGC 2 cut(s) 775, 1172
BlsI GCNGC 2 cut(s) 776, 1173
Bme1390I CCNGG 2 cut(s) 827, 1635
BmeT110I CYCGRG 1 cut(s) 2696
BmgBI CACGTC 1 cut(s) 844
BmiI GGNNCC 1 cut(s) 1966
BmrFI CCNGG 2 cut(s) 827, 1635
BmsI GCATC 8 cut(s) 130, 201, 950, 1207, 1479, 1491, 1762, 2514
BpiI GAAGAC 6 cut(s) 462, 495, 1137, 1581, 2361, 2576
BpmI CTGGAG 1 cut(s) 2483
Bpu14I TTCGAA 2 cut(s) 2257, 2715
BpuEI CTTGAG 2 cut(s) 71, 1816
BpuMI CCSGG 1 cut(s) 1635
BsaBI GATNNNNATC 1 cut(s) 1410
BsaHI GRCGYC 1 cut(s) 1852
BsaJI CCNNGG 2 cut(s) 2297, 2490
BsaWI WCCGGW 1 cut(s) 2221
BsaXI ACNNNNNCTCC 2 cut(s) 2456, 2486
Bsc4I CCNNNNNNNGG 1 cut(s) 1114
Bse1I ACTGG 4 cut(s) 75, 501, 1710, 1966
Bse3DI GCAATG 2 cut(s) 1214, 1279
Bse8I GATNNNNATC 1 cut(s) 1410
BseBI CCWGG 1 cut(s) 827
BseDI CCNNGG 2 cut(s) 2297, 2490
BseGI GGATG 7 cut(s) 145, 539, 550, 941, 1753, 2691, 2723
BseJI GATNNNNATC 1 cut(s) 1410
BseLI CCNNNNNNNGG 1 cut(s) 1114
BseMI GCAATG 2 cut(s) 1214, 1279
BseMII CTCAG 3 cut(s) 1238, 1727, 2113
BseNI ACTGG 4 cut(s) 75, 501, 1710, 1966
BseRI GAGGAG 1 cut(s) 122
BseSI GKGCMC 1 cut(s) 1820
BseXI GCAGC 1 cut(s) 1183
BsgI GTGCAG 2 cut(s) 473, 2234
BshFI GGCC 8 cut(s) 5, 149, 206, 1107, 1474, 1633, 2586, 2750
BsiHKAI GWGCWC 2 cut(s) 1014, 1047
BsiHKCI CYCGRG 1 cut(s) 2696
BsiSI CCGG 2 cut(s) 1634, 2222
BslFI GGGAC 2 cut(s) 2008, 2078
BslI CCNNNNNNNGG 1 cut(s) 1114
BsmAI GTCTC 2 cut(s) 637, 1859
BsmBI CGTCTC 1 cut(s) 1859
BsmFI GGGAC 2 cut(s) 2008, 2078
BsmI GAATGC 2 cut(s) 769, 1028
BsnI GGCC 8 cut(s) 5, 149, 206, 1107, 1474, 1633, 2586, 2750
BsoBI CYCGRG 1 cut(s) 2696
Bsp119I TTCGAA 2 cut(s) 2257, 2715
Bsp1286I GDGCHC 3 cut(s) 1014, 1047, 1820
Bsp143I GATC 7 cut(s) 366, 714, 1162, 1383, 1495, 2671, 2700
BspACI CCGC 3 cut(s) 775, 1858, 2443
BspANI GGCC 8 cut(s) 5, 149, 206, 1107, 1474, 1633, 2586, 2750
BspCNI CTCAG 3 cut(s) 1239, 1726, 2112
BspLI GGNNCC 1 cut(s) 1966
BspMI ACCTGC 2 cut(s) 1941, 2722
BspPI GGATC 3 cut(s) 1170, 1391, 2679
BspQI GCTCTTC 2 cut(s) 543, 1019
BspT104I TTCGAA 2 cut(s) 2257, 2715
BsrDI GCAATG 2 cut(s) 1214, 1279
BsrI ACTGG 4 cut(s) 75, 501, 1710, 1966
BssECI CCNNGG 2 cut(s) 2297, 2490
BssMI GATC 7 cut(s) 366, 714, 1162, 1383, 1495, 2671, 2700
BssNI GRCGYC 1 cut(s) 1852
BssT1I CCWWGG 1 cut(s) 2297
Bst2UI CCWGG 1 cut(s) 827
Bst4CI ACNGT 6 cut(s) 401, 565, 673, 1228, 2556, 2721
Bst6I CTCTTC 6 cut(s) 543, 635, 1019, 1662, 2250, 2424
BstACI GRCGYC 1 cut(s) 1852
BstAPI GCANNNNNTGC 1 cut(s) 2032
BstBI TTCGAA 2 cut(s) 2257, 2715
BstC8I GCNNGC 2 cut(s) 1081, 1512
BstDEI CTNAG 5 cut(s) 1247, 1713, 1943, 2099, 2679
BstF5I GGATG 7 cut(s) 145, 539, 550, 941, 1753, 2691, 2723
BstH2I RGCGCY 1 cut(s) 2006
BstHHI GCGC 2 cut(s) 1938, 2005
BstKTI GATC 7 cut(s) 369, 717, 1165, 1386, 1498, 2674, 2703
BstMAI GTCTC 2 cut(s) 637, 1859
BstMBI GATC 7 cut(s) 366, 714, 1162, 1383, 1495, 2671, 2700
BstMWI GCNNNNNNNGC 6 cut(s) 155, 1124, 1434, 2032, 2082, 2173
BstNI CCWGG 1 cut(s) 827
BstNSI RCATGY 6 cut(s) 250, 1083, 1376, 1441, 1681, 2711
BstSCI CCNGG 2 cut(s) 825, 1633
BstSFI CTRYAG 1 cut(s) 2508
BstSLI GKGCMC 1 cut(s) 1820
BstV1I GCAGC 1 cut(s) 1183
BstV2I GAAGAC 6 cut(s) 462, 495, 1137, 1581, 2361, 2576
BstX2I RGATCY 1 cut(s) 2700
BstYI RGATCY 1 cut(s) 2700
BsuRI GGCC 8 cut(s) 5, 149, 206, 1107, 1474, 1633, 2586, 2750
BtgZI GCGATG 1 cut(s) 782
BtrI CACGTC 1 cut(s) 844
BtsCI GGATG 7 cut(s) 145, 539, 550, 941, 1753, 2691, 2723
BtsI GCAGTG 2 cut(s) 2309, 2381
BtsIMutI CAGTG 5 cut(s) 82, 1224, 1703, 2309, 2381
BveI ACCTGC 2 cut(s) 1941, 2722
Cac8I GCNNGC 2 cut(s) 1081, 1512
CfoI GCGC 2 cut(s) 1938, 2005
CseI GACGC 1 cut(s) 1841
CsiI ACCWGGT 1 cut(s) 825
Csp6I GTAC 5 cut(s) 397, 695, 2294, 2363, 2684
CviQI GTAC 5 cut(s) 397, 695, 2294, 2363, 2684
DdeI CTNAG 5 cut(s) 1247, 1713, 1943, 2099, 2679
DpnI GATC 7 cut(s) 368, 716, 1164, 1385, 1497, 2673, 2702
DpnII GATC 7 cut(s) 366, 714, 1162, 1383, 1495, 2671, 2700
DraI TTTAAA 1 cut(s) 2043
EaeI YGGCCR 1 cut(s) 1631
Eam1104I CTCTTC 6 cut(s) 543, 635, 1019, 1662, 2250, 2424
EarI CTCTTC 6 cut(s) 543, 635, 1019, 1662, 2250, 2424
Ecl136II GAGCTC 1 cut(s) 1012
Eco130I CCWWGG 1 cut(s) 2297
Eco147I AGGCCT 2 cut(s) 1107, 2586
Eco24I GRGCYC 1 cut(s) 1014
Eco32I GATATC 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 1012
Eco57I CTGAAG 4 cut(s) 186, 453, 1013, 1377
Eco88I CYCGRG 1 cut(s) 2696
EcoICRI GAGCTC 1 cut(s) 1012
EcoRII CCWGG 1 cut(s) 825
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 2297
EcoT22I ATGCAT 3 cut(s) 664, 1494, 2419
EcoT38I GRGCYC 1 cut(s) 1014
ErhI CCWWGG 1 cut(s) 2297
Esp3I CGTCTC 1 cut(s) 1859
FaqI GGGAC 2 cut(s) 2008, 2078
FauNDI CATATG 1 cut(s) 2419
FbaI TGATCA 1 cut(s) 714
FblI GTMKAC 3 cut(s) 271, 1520, 2736
Fnu4HI GCNGC 2 cut(s) 775, 1172
FokI GGATG 7 cut(s) 152, 546, 557, 928, 1760, 2678, 2710
FriOI GRGCYC 1 cut(s) 1014
Fsp4HI GCNGC 2 cut(s) 775, 1172
GlaI GCGC 2 cut(s) 1937, 2004
GluI GCNGC 2 cut(s) 775, 1172
GsuI CTGGAG 1 cut(s) 2483
HaeII RGCGCY 1 cut(s) 2006
HaeIII GGCC 8 cut(s) 5, 149, 206, 1107, 1474, 1633, 2586, 2750
HapII CCGG 2 cut(s) 1634, 2222
HgaI GACGC 1 cut(s) 1841
HhaI GCGC 2 cut(s) 1938, 2005
Hin1I GRCGYC 1 cut(s) 1852
Hin6I GCGC 2 cut(s) 1936, 2003
HinP1I GCGC 2 cut(s) 1936, 2003
HincII GTYRAC 3 cut(s) 272, 1521, 1959
HindII GTYRAC 3 cut(s) 272, 1521, 1959
HindIII AAGCTT 2 cut(s) 709, 2261
HpaII CCGG 2 cut(s) 1634, 2222
HphI GGTGA 4 cut(s) 649, 965, 1378, 2506
Hpy166II GTNNAC 8 cut(s) 272, 697, 973, 1058, 1521, 1959, 2240, 2737
Hpy188I TCNGA 7 cut(s) 197, 433, 902, 1500, 2059, 2394, 2658
Hpy8I GTNNAC 8 cut(s) 272, 697, 973, 1058, 1521, 1959, 2240, 2737
Hpy99I CGWCG 2 cut(s) 1559, 1593
HpyCH4III ACNGT 6 cut(s) 401, 565, 673, 1228, 2556, 2721
HpyCH4IV ACGT 3 cut(s) 328, 843, 2289
HpyF10VI GCNNNNNNNGC 6 cut(s) 155, 1124, 1434, 2032, 2082, 2173
HpyF3I CTNAG 5 cut(s) 1247, 1713, 1943, 2099, 2679
HpySE526I ACGT 3 cut(s) 328, 843, 2289
Hsp92I GRCGYC 1 cut(s) 1852
HspAI GCGC 2 cut(s) 1936, 2003
Ksp22I TGATCA 1 cut(s) 714
Kzo9I GATC 7 cut(s) 366, 714, 1162, 1383, 1495, 2671, 2700
LguI GCTCTTC 2 cut(s) 543, 1019
LmnI GCTCC 3 cut(s) 109, 419, 1009
Lsp1109I GCAGC 1 cut(s) 1183
LweI GCATC 8 cut(s) 130, 201, 950, 1207, 1479, 1491, 1762, 2514
MabI ACCWGGT 1 cut(s) 825
MaeII ACGT 3 cut(s) 328, 843, 2289
MaeIII GTNAC 5 cut(s) 13, 345, 792, 916, 1440
MalI GATC 7 cut(s) 368, 716, 1164, 1385, 1497, 2673, 2702
MboI GATC 7 cut(s) 366, 714, 1162, 1383, 1495, 2671, 2700
MfeI CAATTG 1 cut(s) 1271
MflI RGATCY 1 cut(s) 2700
MhlI GDGCHC 3 cut(s) 1014, 1047, 1820
MlyI GAGTC 6 cut(s) 277, 476, 821, 1726, 1850, 2086
MmeI TCCRAC 1 cut(s) 1381
Mph1103I ATGCAT 3 cut(s) 664, 1494, 2419
MroXI GAANNNNTTC 4 cut(s) 128, 438, 1107, 2217
MslI CAYNNNNRTG 3 cut(s) 249, 702, 1078
MspI CCGG 2 cut(s) 1634, 2222
MspR9I CCNGG 2 cut(s) 827, 1635
MunI CAATTG 1 cut(s) 1271
Mva1269I GAATGC 2 cut(s) 769, 1028
MvaI CCWGG 1 cut(s) 827
MwoI GCNNNNNNNGC 6 cut(s) 155, 1124, 1434, 2032, 2082, 2173
NciI CCSGG 1 cut(s) 1635
NdeI CATATG 1 cut(s) 2419
NdeII GATC 7 cut(s) 366, 714, 1162, 1383, 1495, 2671, 2700
NlaIV GGNNCC 1 cut(s) 1966
NmeAIII GCCGAG 3 cut(s) 490, 1500, 1552
NsiI ATGCAT 3 cut(s) 664, 1494, 2419
NspI RCATGY 6 cut(s) 250, 1083, 1376, 1441, 1681, 2711
NspV TTCGAA 2 cut(s) 2257, 2715
OliI CACNNNNGTG 1 cut(s) 702
PacI TTAATTAA 1 cut(s) 30
PaeI GCATGC 1 cut(s) 1083
PceI AGGCCT 2 cut(s) 1107, 2586
PciI ACATGT 4 cut(s) 246, 1372, 1677, 2707
PciSI GCTCTTC 2 cut(s) 543, 1019
PctI GAATGC 2 cut(s) 769, 1028
PdmI GAANNNNTTC 4 cut(s) 128, 438, 1107, 2217
PfeI GAWTC 4 cut(s) 388, 909, 1648, 2054
PkrI GCNGC 2 cut(s) 776, 1173
PleI GAGTC 6 cut(s) 276, 475, 820, 1726, 1849, 2086
PpsI GAGTC 6 cut(s) 276, 475, 820, 1726, 1849, 2086
PscI ACATGT 4 cut(s) 246, 1372, 1677, 2707
Psp124BI GAGCTC 1 cut(s) 1014
Psp6I CCWGG 1 cut(s) 825
PspGI CCWGG 1 cut(s) 825
PspN4I GGNNCC 1 cut(s) 1966
PsuI RGATCY 1 cut(s) 2700
RsaI GTAC 5 cut(s) 398, 696, 2295, 2364, 2685
RsaNI GTAC 5 cut(s) 397, 695, 2294, 2363, 2684
RseI CAYNNNNRTG 3 cut(s) 249, 702, 1078
SacI GAGCTC 1 cut(s) 1014
SalI GTCGAC 2 cut(s) 270, 1519
SapI GCTCTTC 2 cut(s) 543, 1019
SatI GCNGC 2 cut(s) 775, 1172
Sau3AI GATC 7 cut(s) 366, 714, 1162, 1383, 1495, 2671, 2700
SchI GAGTC 6 cut(s) 277, 476, 821, 1726, 1850, 2086
ScrFI CCNGG 2 cut(s) 827, 1635
SduI GDGCHC 3 cut(s) 1014, 1047, 1820
SexAI ACCWGGT 1 cut(s) 825
SfaNI GCATC 8 cut(s) 130, 201, 950, 1207, 1479, 1491, 1762, 2514
SfcI CTRYAG 1 cut(s) 2508
SfuI TTCGAA 2 cut(s) 2257, 2715
SmiMI CAYNNNNRTG 3 cut(s) 249, 702, 1078
SmlI CTYRAG 2 cut(s) 50, 1795
SmoI CTYRAG 2 cut(s) 50, 1795
SpeI ACTAGT 1 cut(s) 796
SphI GCATGC 1 cut(s) 1083
SseBI AGGCCT 2 cut(s) 1107, 2586
SsiI CCGC 3 cut(s) 775, 1858, 2443
SspI AATATT 1 cut(s) 2014
SstI GAGCTC 1 cut(s) 1014
StuI AGGCCT 2 cut(s) 1107, 2586
StyD4I CCNGG 2 cut(s) 825, 1633
StyI CCWWGG 1 cut(s) 2297
TaaI ACNGT 6 cut(s) 401, 565, 673, 1228, 2556, 2721
TaiI ACGT 3 cut(s) 331, 846, 2292
TaqII GACCGA 1 cut(s) 2664
TatI WGTACW 1 cut(s) 2362
TauI GCSGC 1 cut(s) 777
TfiI GAWTC 4 cut(s) 388, 909, 1648, 2054
TscAI CASTG 5 cut(s) 82, 1231, 1710, 2316, 2388
TseI GCWGC 1 cut(s) 1171
TspDTI ATGAA 9 cut(s) 72, 102, 137, 226, 469, 561, 1203, 2220, 2226
TspGWI ACGGA 1 cut(s) 1421
TspRI CASTG 5 cut(s) 82, 1231, 1710, 2316, 2388
XapI RAATTY 4 cut(s) 233, 757, 1807, 2482
XbaI TCTAGA 3 cut(s) 835, 1201, 1735
XceI RCATGY 6 cut(s) 250, 1083, 1376, 1441, 1681, 2711
XcmI CCANNNNNNNNNTGG 3 cut(s) 315, 823, 1156
XmiI GTMKAC 3 cut(s) 271, 1520, 2736
XmnI GAANNNNTTC 4 cut(s) 128, 438, 1107, 2217
Zsp2I ATGCAT 3 cut(s) 664, 1494, 2419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.