RchiOBHm_Chr5g0052661

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000110
Physical Location & Seq
Forward (+)
54643805 .. 54647755
3951 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33005

Sequence Viewer

Length: 2784 bp
ATGGCCTTGTCTGTAACGGACCTCTTAATTGGAAAACTTGCGACCATTCTTGAGTACGAAGGAACAACCATAGCAGGTGTTCGTCATGAAGTTGATAAGATTAAGGAGGAGTTTTTAAGCATGAAAGCTTTCCTAGCGGATGCGGAGGCCAACAAACCAAAAACTGAAGGACAGAAATTGTGGGTTGCAAGAATCAGAGACTTAGCCTATGATGTTGAAGATATCATTGATGAATTCATGTATCACATGTATGCGAAGCAGACTGGGGGTCGACTTTCAAGGGGGCTACACAAAACCATTCGCGCTCCATGTAATCTTTGGTTCAGCCATAAAATTGCAAAGAAGTTGCAGAAAATTACTGACGTGATCAAAGCCATTCCAGAGAGGAATCAAAGATACGGTGTTGGCCTTGTAGGAGGAGGAGGAGGAGCAACTACATCCGACGATATTCAGAAATTGGTGCAGAACCAAGCGGAGTCTTCGTTTTTCATTATGGAAGACGAACTGGTTGGGATTGAAGGCAAGAAGCAAATACTAATGGGATGGCTAATGGATGATGAGCAACACCAAACTGTTATATCTGTGGTGGGCATGGGAGGTTCTGGAAAGACAACTCTAGTTGCCAAGACCTTCACCAATGAACTGGTAAAGAGACATTTTGATTGTTATGCATGGATTACTGTTTCCCAAACTTATGATGCGGTACACTTGTTTCGAAGCTTGATCAGGGAACTCTACAAATCAAGGAAGGAAAATTTCCCTACAATTTTGAATGCCATGAGCCGCATAGAATTGCTAGAGTTACTAGTTAACTACTTGGAGTCCAGAAAATACCTAGTTGTTCTAGATGACGTGTGGGATATAAAAGTATGGAGAGAAATAAAGGTACCACTTCAAGATAGACACCTTGGAAGTCGGGTCGTACTCACAACTCGAAATGAAGAAGTAGCATCCAACTCTTTTGGAGTTGAAAGCTATGTTTACCGTATTCAGCCCCTGCAAAAGAATGAGGCGTGGGAACTATTCAGTAGGAAAGCATTCTCAACTAAGCAACACAAAACTTGTCCAACTGAGCTGAAGTCATTAGCCTGCCAACTTGTGGAGAAGTGTGAAGGCCTTCCTCTCGGTATTGTGGCTTTAGGTGGTTTTATGTCTTCTAAGAAGTCGCTGGATCAATGGCAACAAGTCTGCAACAACTTGAATTGGTATCTGCATAACAATTCATCGCTAGATCCTGTGAGAAACATTTTGTTGCTCAGTTTCAATGATTTGCCATCTCAACTGAAGCATTGCTTCCTCTATTGTTCCCTTTTCCCTGAAGATTATCTATTTAAAAGCAAAAGGCTGACTAGATTGTGGATAGCGGAAGGATTTGTTGAAAATATGAAAGGGGTCACGCCCGAAGAAGTTGCAGATGGCTATCTTCGGGAACTCTGCTTCCTTAGCATGTTACAGGTTGCAGATGACTTTGTTTCTGTAACATCTTCAGGAACATCTTCGGGAAGGCCGAAAAAATTTAAGATGCATGATCTGATGCGAGAGTTTTCTTTGTCGACAGCCGAAAAAGAAAAGTTTGGTTATGTATACAACGGGAGAGAAGTAATGGAAGAGATCTCAACCCGTCGCTTGTCAATTCACAGAATGGAAGGAGAAATTAAATCATGGCGGGGTATGTCCAAAATTCGTTCTCTTCTTGTATTTGACACTGACATGTCCTCATTATCTTTCTTAAATACACTGGTTTCTCGATTCAAATTGTTGAGGACTCTAGACTTGGAGGATGTCCAAATTGATAATCTGCCGGGTGCAGTTGTTTACTTGTTCAACTTGAGATATTTAAATTTGAAGGGCACTTTAATTGAGGAACTTCCAGAGTCCATAAGGTGTCTCCGCAACCTTCAATTTTTGAACATCAGGGATACTAAGATAGAGTCACTTCCACAAGGAATTATGAGGTTGCTGAACCTGCGCAATCTAAGTATGTTTCGCTACACTTGGAACCTGTACTTCAATGTTGTGCGTGGGACAAAAGCGCCATCAAATATTTGTAAGTTGCAGAAATTGCAAAGTTTATCAATAATTGAATCAGAAGGGGACACTTGTAGACTTATCGCCAACATGACTCAACTTAAGAGCATCTGCATTTCGAATGTGAAAGAAAGAGATGAGATTGACCTTTGGGTCTCAATTAAGAAGTTCACTCTGCTTCAATCTCTAGGTTTAATGGCAAGTAATGAAGAGGAAATCCTTCCGGTGAAAGCACAATGTCCTCCTCTTCCGCACCTTCGAAAGCTTACTTTGTTTGGCAGACTGCAAAACGTAACACCTTGGTTTTCTTCACTGCATAGCCTCACAGCTCTGTTTCTGCATTGGTCAAGACTTGAAGAGGATTTACTACCTCAAATTGAAGCACTGCTCAATCTGACAAGGCTTTGTCTTTGTAATGCGTATGTTGGGGATGAGTTGTGTTTCCATAGAGGCTTTGTAAAGCTTATGAGGTTGGAGTTGTTGAATTTTGCTTCGGTGAAAAAGATAACTATAGAAAATGGGGTGATGCCAAATCTCCGGTACTTATGCGTTGACAGCTTCATGGAGTTGAAGACAGTGCCATTGGGCCTTGAGTATCTTTCTACTCTACAATATTTGGGCCTAAAAGATGTTCCCATAGAAGTTATAAGGCCCATTCAGAAAGGAGGTGTGGATCGCTCTAAGGTACAACACATTCCTGAGATCATACATGTTTTTGAACAGTCATCCAACTGGATCCACGAAAACTTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

927

Amino Acids

106.37

Weight (kDa)

8.43

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 87 1.5e-22 Rx N-terminal domain
NB-ARC PF00931 175 - 350 4.2e-47 NB-ARC domain
WHD_DRP PF23559 437 - 515 4.4e-18 Disease resistance protein Winged helix domain
LRR_14 PF23598 560 - 881 1.2e-36 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2675
AarI CACCTGC 1 cut(s) 65
AasI GACNNNNNNGTC 1 cut(s) 2180
Acc16I TGCGCA 1 cut(s) 1970
Acc36I ACCTGC 2 cut(s) 65, 1974
Acc65I GGTACC 1 cut(s) 886
AccB1I GGYRCC 1 cut(s) 886
AccB7I CCANNNNNTGG 1 cut(s) 1099
AccI GTMKAC 4 cut(s) 271, 1553, 1584, 2104
AccII CGCG 1 cut(s) 303
AciI CCGC 9 cut(s) 137, 143, 473, 701, 784, 1364, 1666, 1891, 2279
AclWI GGATC 5 cut(s) 1179, 1226, 2709, 2758, 2771
AcsI RAATTY 6 cut(s) 233, 754, 1514, 1680, 1840, 2512
AcuI CTGAAG 5 cut(s) 186, 1097, 1304, 1338, 1470
AfaI GTAC 7 cut(s) 56, 705, 888, 924, 2006, 2570, 2715
AfiI CCNNNNNNNGG 1 cut(s) 1099
AflII CTTAAG 1 cut(s) 2129
AflIII ACRYGT 4 cut(s) 246, 852, 1710, 2737
AhdI GACNNNNNGTC 1 cut(s) 267
AhlI ACTAGT 1 cut(s) 805
AjiI CACGTC 2 cut(s) 364, 853
AloI GAACNNNNNNTCC 2 cut(s) 1422, 1454
AluBI AGCT 8 cut(s) 128, 720, 975, 1075, 2293, 2357, 2491, 2586
AluI AGCT 8 cut(s) 128, 720, 975, 1075, 2293, 2357, 2491, 2586
Alw26I GTCTC 4 cut(s) 192, 646, 1892, 2188
AlwI GGATC 5 cut(s) 1179, 1226, 2709, 2758, 2771
AlwNI CAGNNNCTG 1 cut(s) 997
AoxI GGCC 9 cut(s) 3, 147, 406, 1114, 1505, 2614, 2647, 2678, 2778
ApoI RAATTY 6 cut(s) 233, 754, 1514, 1680, 1840, 2512
Asp700I GAANNNNTTC 5 cut(s) 128, 1037, 1116, 1495, 2247
Asp718I GGTACC 1 cut(s) 886
AspLEI GCGC 3 cut(s) 305, 1971, 2035
AspS9I GGNCC 4 cut(s) 19, 2614, 2647, 2679
AsuC2I CCSGG 1 cut(s) 1803
AsuHPI GGTGA 4 cut(s) 625, 2266, 2536, 2563
AsuII TTCGAA 3 cut(s) 715, 2147, 2287
AvaII GGWCC 1 cut(s) 19
BaeGI GKGCMC 1 cut(s) 1853
BaeI ACNNNNGTAYC 2 cut(s) 695, 728
BamHI GGATCC 1 cut(s) 2763
BanI GGYRCC 1 cut(s) 886
BarI GAAGNNNNNNTAC 2 cut(s) 2376, 2408
BbsI GAAGAC 4 cut(s) 471, 504, 1146, 2606
BccI CCATC 4 cut(s) 537, 1282, 1409, 2044
BcgI CGANNNNNNTGC 2 cut(s) 1391, 1425
BciVI GTATCC 1 cut(s) 1912
BclI TGATCA 2 cut(s) 366, 723
BcnI CCSGG 1 cut(s) 1803
BcoDI GTCTC 4 cut(s) 192, 646, 1892, 2188
BcuI ACTAGT 1 cut(s) 805
BfmI CTRYAG 1 cut(s) 2538
BfoI RGCGCY 1 cut(s) 2036
BfrI CTTAAG 1 cut(s) 2129
BfuAI ACCTGC 2 cut(s) 65, 1974
BfuI GTATCC 1 cut(s) 1912
BglII AGATCT 1 cut(s) 1611
BisI GCNGC 1 cut(s) 784
BlsI GCNGC 1 cut(s) 785
Bme1390I CCNGG 1 cut(s) 1803
Bme18I GGWCC 1 cut(s) 19
BmeRI GACNNNNNGTC 1 cut(s) 267
BmgBI CACGTC 2 cut(s) 364, 853
BmgT120I GGNCC 4 cut(s) 19, 2614, 2647, 2679
BmiI GGNNCC 3 cut(s) 888, 2000, 2765
BmrFI CCNGG 1 cut(s) 1803
BmrI ACTGGG 1 cut(s) 273
BmsI GCATC 7 cut(s) 130, 688, 959, 1512, 1524, 2145, 2544
BmuI ACTGGG 1 cut(s) 273
BpiI GAAGAC 4 cut(s) 471, 504, 1146, 2606
Bpu10I CCTNAGC 1 cut(s) 1442
Bpu14I TTCGAA 3 cut(s) 715, 2147, 2287
BpuEI CTTGAG 3 cut(s) 71, 1849, 2639
BpuMI CCSGG 1 cut(s) 1803
BsaBI GATNNNNATC 1 cut(s) 1419
BsaI GGTCTC 1 cut(s) 2188
BsaJI CCNNGG 2 cut(s) 907, 2327
BsaWI WCCGGW 2 cut(s) 2251, 2565
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 1099
Bse1I ACTGG 5 cut(s) 268, 510, 648, 1743, 2765
Bse3DI GCAATG 1 cut(s) 1288
Bse8I GATNNNNATC 1 cut(s) 1419
BseDI CCNNGG 2 cut(s) 907, 2327
BseGI GGATG 8 cut(s) 145, 437, 548, 559, 950, 1786, 2464, 2753
BseJI GATNNNNATC 1 cut(s) 1419
BseLI CCNNNNNNNGG 1 cut(s) 1099
BseMI GCAATG 1 cut(s) 1288
BseMII CTCAG 3 cut(s) 1062, 1270, 2718
BseNI ACTGG 5 cut(s) 268, 510, 648, 1743, 2765
BseRI GAGGAG 6 cut(s) 122, 432, 435, 438, 441, 2262
BseSI GKGCMC 1 cut(s) 1853
BsgI GTGCAG 2 cut(s) 482, 1827
Bsh1236I CGCG 1 cut(s) 303
BshFI GGCC 9 cut(s) 5, 149, 408, 1116, 1507, 2616, 2649, 2680, 2780
BshNI GGYRCC 1 cut(s) 886
BsiSI CCGG 3 cut(s) 1802, 2252, 2566
BslFI GGGAC 2 cut(s) 2038, 2108
BslI CCNNNNNNNGG 1 cut(s) 1099
BsmAI GTCTC 4 cut(s) 192, 646, 1892, 2188
BsmFI GGGAC 2 cut(s) 2038, 2108
BsmI GAATGC 2 cut(s) 778, 1037
BsnI GGCC 9 cut(s) 5, 149, 408, 1116, 1507, 2616, 2649, 2680, 2780
Bso31I GGTCTC 1 cut(s) 2188
Bsp119I TTCGAA 3 cut(s) 715, 2147, 2287
Bsp1286I GDGCHC 1 cut(s) 1853
Bsp143I GATC 9 cut(s) 366, 723, 1171, 1231, 1528, 1611, 2701, 2730, 2763
BspACI CCGC 9 cut(s) 137, 143, 473, 701, 784, 1364, 1666, 1891, 2279
BspANI GGCC 9 cut(s) 5, 149, 408, 1116, 1507, 2616, 2649, 2680, 2780
BspCNI CTCAG 3 cut(s) 1063, 1269, 2719
BspFNI CGCG 1 cut(s) 303
BspHI TCATGA 1 cut(s) 85
BspLI GGNNCC 3 cut(s) 888, 2000, 2765
BspMI ACCTGC 2 cut(s) 65, 1974
BspPI GGATC 5 cut(s) 1179, 1226, 2709, 2758, 2771
BspT104I TTCGAA 3 cut(s) 715, 2147, 2287
BspT107I GGYRCC 1 cut(s) 886
BspTI CTTAAG 1 cut(s) 2129
BspTNI GGTCTC 1 cut(s) 2188
BsrDI GCAATG 1 cut(s) 1288
BsrI ACTGG 5 cut(s) 268, 510, 648, 1743, 2765
BssECI CCNNGG 2 cut(s) 907, 2327
BssMI GATC 9 cut(s) 366, 723, 1171, 1231, 1528, 1611, 2701, 2730, 2763
BssNAI GTATAC 1 cut(s) 1585
BssT1I CCWWGG 2 cut(s) 907, 2327
Bst1107I GTATAC 1 cut(s) 1585
Bst4CI ACNGT 6 cut(s) 401, 574, 682, 986, 2605, 2751
Bst6I CTCTTC 5 cut(s) 1602, 1695, 2232, 2280, 2379
BstAFI CTTAAG 1 cut(s) 2129
BstAPI GCANNNNNTGC 1 cut(s) 2062
BstBI TTCGAA 3 cut(s) 715, 2147, 2287
BstC8I GCNNGC 1 cut(s) 1090
BstF5I GGATG 8 cut(s) 145, 437, 548, 559, 950, 1786, 2464, 2753
BstFNI CGCG 1 cut(s) 303
BstH2I RGCGCY 1 cut(s) 2036
BstHHI GCGC 3 cut(s) 305, 1971, 2035
BstKTI GATC 9 cut(s) 369, 726, 1174, 1234, 1531, 1614, 2704, 2733, 2766
BstMAI GTCTC 4 cut(s) 192, 646, 1892, 2188
BstMBI GATC 9 cut(s) 366, 723, 1171, 1231, 1528, 1611, 2701, 2730, 2763
BstMWI GCNNNNNNNGC 5 cut(s) 134, 1443, 1966, 2062, 2583
BstNSI RCATGY 4 cut(s) 250, 1450, 1714, 2741
BstSCI CCNGG 1 cut(s) 1801
BstSFI CTRYAG 1 cut(s) 2538
BstSLI GKGCMC 1 cut(s) 1853
BstUI CGCG 1 cut(s) 303
BstV2I GAAGAC 4 cut(s) 471, 504, 1146, 2606
BstX2I RGATCY 3 cut(s) 1231, 1611, 2763
BstXI CCANNNNNNTGG 1 cut(s) 643
BstYI RGATCY 3 cut(s) 1231, 1611, 2763
BstZ17I GTATAC 1 cut(s) 1585
BsuI GTATCC 1 cut(s) 1912
BsuRI GGCC 9 cut(s) 5, 149, 408, 1116, 1507, 2616, 2649, 2680, 2780
BtgZI GCGATG 1 cut(s) 1209
BtrI CACGTC 2 cut(s) 364, 853
BtsCI GGATG 8 cut(s) 145, 437, 548, 559, 950, 1786, 2464, 2753
BtsI GCAGTG 2 cut(s) 2339, 2411
BtsIMutI CAGTG 5 cut(s) 1704, 1736, 2339, 2411, 2610
BveI ACCTGC 2 cut(s) 65, 1974
Cac8I GCNNGC 1 cut(s) 1090
CaiI CAGNNNCTG 1 cut(s) 997
CciI TCATGA 1 cut(s) 85
CfoI GCGC 3 cut(s) 305, 1971, 2035
Cfr13I GGNCC 4 cut(s) 19, 2614, 2647, 2679
Csp6I GTAC 7 cut(s) 55, 704, 887, 923, 2005, 2569, 2714
CviQI GTAC 7 cut(s) 55, 704, 887, 923, 2005, 2569, 2714
DpnI GATC 9 cut(s) 368, 725, 1173, 1233, 1530, 1613, 2703, 2732, 2765
DpnII GATC 9 cut(s) 366, 723, 1171, 1231, 1528, 1611, 2701, 2730, 2763
DraI TTTAAA 2 cut(s) 1333, 1839
DrdI GACNNNNNNGTC 1 cut(s) 2180
DriI GACNNNNNGTC 1 cut(s) 267
DseDI GACNNNNNNGTC 1 cut(s) 2180
Eam1104I CTCTTC 5 cut(s) 1602, 1695, 2232, 2280, 2379
Eam1105I GACNNNNNGTC 1 cut(s) 267
EarI CTCTTC 5 cut(s) 1602, 1695, 2232, 2280, 2379
Eco130I CCWWGG 2 cut(s) 907, 2327
Eco147I AGGCCT 1 cut(s) 1116
Eco31I GGTCTC 1 cut(s) 2188
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 19
Eco57I CTGAAG 5 cut(s) 186, 1097, 1304, 1338, 1470
EcoRI GAATTC 1 cut(s) 233
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 2 cut(s) 907, 2327
EcoT22I ATGCAT 2 cut(s) 673, 1527
ErhI CCWWGG 2 cut(s) 907, 2327
FalI AAGNNNNNCTT 2 cut(s) 1277, 1309
FaqI GGGAC 2 cut(s) 2038, 2108
FauI CCCGC 1 cut(s) 1659
FbaI TGATCA 2 cut(s) 366, 723
FblI GTMKAC 4 cut(s) 271, 1553, 1584, 2104
Fnu4HI GCNGC 1 cut(s) 784
FokI GGATG 8 cut(s) 152, 424, 555, 566, 937, 1793, 2471, 2740
Fsp4HI GCNGC 1 cut(s) 784
FspI TGCGCA 1 cut(s) 1970
GlaI GCGC 3 cut(s) 304, 1970, 2034
GluI GCNGC 1 cut(s) 784
HaeII RGCGCY 1 cut(s) 2036
HaeIII GGCC 9 cut(s) 5, 149, 408, 1116, 1507, 2616, 2649, 2680, 2780
HapII CCGG 3 cut(s) 1802, 2252, 2566
HhaI GCGC 3 cut(s) 305, 1971, 2035
Hin6I GCGC 3 cut(s) 303, 1969, 2033
HinP1I GCGC 3 cut(s) 303, 1969, 2033
HincII GTYRAC 4 cut(s) 272, 811, 1554, 2581
HindII GTYRAC 4 cut(s) 272, 811, 1554, 2581
HindIII AAGCTT 4 cut(s) 126, 718, 2291, 2489
HpaI GTTAAC 1 cut(s) 811
HpaII CCGG 3 cut(s) 1802, 2252, 2566
HphI GGTGA 4 cut(s) 625, 2266, 2536, 2563
Hpy188I TCNGA 7 cut(s) 197, 442, 453, 1533, 2089, 2424, 2688
Hpy99I CGWCG 2 cut(s) 446, 1626
HpyCH4III ACNGT 6 cut(s) 401, 574, 682, 986, 2605, 2751
HpyCH4IV ACGT 3 cut(s) 363, 852, 2319
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 1443, 1966, 2062, 2583
HpySE526I ACGT 3 cut(s) 363, 852, 2319
HspAI GCGC 3 cut(s) 303, 1969, 2033
KpnI GGTACC 1 cut(s) 890
Ksp22I TGATCA 2 cut(s) 366, 723
KspAI GTTAAC 1 cut(s) 811
Kzo9I GATC 9 cut(s) 366, 723, 1171, 1231, 1528, 1611, 2701, 2730, 2763
LmnI GCTCC 2 cut(s) 310, 428
LweI GCATC 7 cut(s) 130, 688, 959, 1512, 1524, 2145, 2544
MaeII ACGT 3 cut(s) 363, 852, 2319
MaeIII GTNAC 7 cut(s) 13, 801, 1393, 1449, 1477, 1932, 2320
MalI GATC 9 cut(s) 368, 725, 1173, 1233, 1530, 1613, 2703, 2732, 2765
MboI GATC 9 cut(s) 366, 723, 1171, 1231, 1528, 1611, 2701, 2730, 2763
MflI RGATCY 3 cut(s) 1231, 1611, 2763
MhlI GDGCHC 1 cut(s) 1853
MlyI GAGTC 6 cut(s) 485, 830, 1759, 1883, 1940, 2116
MmeI TCCRAC 5 cut(s) 465, 978, 1091, 2481, 2781
Mph1103I ATGCAT 2 cut(s) 673, 1527
MroXI GAANNNNTTC 5 cut(s) 128, 1037, 1116, 1495, 2247
MslI CAYNNNNRTG 2 cut(s) 249, 1709
MspCI CTTAAG 1 cut(s) 2129
MspI CCGG 3 cut(s) 1802, 2252, 2566
MspR9I CCNGG 1 cut(s) 1803
Mva1269I GAATGC 2 cut(s) 778, 1037
MvnI CGCG 1 cut(s) 303
MwoI GCNNNNNNNGC 5 cut(s) 134, 1443, 1966, 2062, 2583
NciI CCSGG 1 cut(s) 1803
NdeII GATC 9 cut(s) 366, 723, 1171, 1231, 1528, 1611, 2701, 2730, 2763
NlaIV GGNNCC 3 cut(s) 888, 2000, 2765
NmuCI GTSAC 2 cut(s) 1393, 1932
NsbI TGCGCA 1 cut(s) 1970
NsiI ATGCAT 2 cut(s) 673, 1527
NspI RCATGY 4 cut(s) 250, 1450, 1714, 2741
NspV TTCGAA 3 cut(s) 715, 2147, 2287
PagI TCATGA 1 cut(s) 85
PaqCI CACCTGC 1 cut(s) 65
PceI AGGCCT 1 cut(s) 1116
PciI ACATGT 3 cut(s) 246, 1710, 2737
PcsI WCGNNNNNNNCGW 1 cut(s) 1505
PctI GAATGC 2 cut(s) 778, 1037
PdmI GAANNNNTTC 5 cut(s) 128, 1037, 1116, 1495, 2247
PfeI GAWTC 4 cut(s) 192, 388, 1749, 2084
PflMI CCANNNNNTGG 1 cut(s) 1099
PkrI GCNGC 1 cut(s) 785
PleI GAGTC 6 cut(s) 484, 829, 1759, 1882, 1939, 2116
PpsI GAGTC 6 cut(s) 484, 829, 1759, 1882, 1939, 2116
PscI ACATGT 3 cut(s) 246, 1710, 2737
PsiI TTATAA 1 cut(s) 2675
PspN4I GGNNCC 3 cut(s) 888, 2000, 2765
PspPI GGNCC 4 cut(s) 19, 2614, 2647, 2679
PstNI CAGNNNCTG 1 cut(s) 997
PsuI RGATCY 3 cut(s) 1231, 1611, 2763
RsaI GTAC 7 cut(s) 56, 705, 888, 924, 2006, 2570, 2715
RsaNI GTAC 7 cut(s) 55, 704, 887, 923, 2005, 2569, 2714
RseI CAYNNNNRTG 2 cut(s) 249, 1709
SalI GTCGAC 2 cut(s) 270, 1552
SatI GCNGC 1 cut(s) 784
Sau3AI GATC 9 cut(s) 366, 723, 1171, 1231, 1528, 1611, 2701, 2730, 2763
Sau96I GGNCC 4 cut(s) 19, 2614, 2647, 2679
SchI GAGTC 6 cut(s) 485, 830, 1759, 1883, 1940, 2116
ScrFI CCNGG 1 cut(s) 1803
SduI GDGCHC 1 cut(s) 1853
SfaNI GCATC 7 cut(s) 130, 688, 959, 1512, 1524, 2145, 2544
SfcI CTRYAG 1 cut(s) 2538
SfuI TTCGAA 3 cut(s) 715, 2147, 2287
SinI GGWCC 1 cut(s) 19
SmiI ATTTAAAT 1 cut(s) 1839
SmiMI CAYNNNNRTG 2 cut(s) 249, 1709
SmlI CTYRAG 4 cut(s) 50, 1828, 2129, 2618
SmoI CTYRAG 4 cut(s) 50, 1828, 2129, 2618
SpeI ACTAGT 1 cut(s) 805
SseBI AGGCCT 1 cut(s) 1116
SsiI CCGC 9 cut(s) 137, 143, 473, 701, 784, 1364, 1666, 1891, 2279
SspI AATATT 2 cut(s) 2044, 2642
StuI AGGCCT 1 cut(s) 1116
StyD4I CCNGG 1 cut(s) 1801
StyI CCWWGG 2 cut(s) 907, 2327
SwaI ATTTAAAT 1 cut(s) 1839
TaaI ACNGT 6 cut(s) 401, 574, 682, 986, 2605, 2751
TaiI ACGT 3 cut(s) 366, 855, 2322
TaqI TCGA 7 cut(s) 271, 715, 934, 1553, 1747, 2147, 2287
TatI WGTACW 1 cut(s) 2004
TauI GCSGC 1 cut(s) 786
TfiI GAWTC 4 cut(s) 192, 388, 1749, 2084
TscAI CASTG 5 cut(s) 1711, 1743, 2346, 2418, 2610
TseFI GTSAC 2 cut(s) 1393, 1932
Tsp45I GTSAC 2 cut(s) 1393, 1932
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 5 cut(s) 1711, 1743, 2346, 2418, 2610
Van91I CCANNNNNTGG 1 cut(s) 1099
Vha464I CTTAAG 1 cut(s) 2129
VpaK11BI GGWCC 1 cut(s) 19
XapI RAATTY 6 cut(s) 233, 754, 1514, 1680, 1840, 2512
XbaI TCTAGA 2 cut(s) 844, 1768
XceI RCATGY 4 cut(s) 250, 1450, 1714, 2741
XcmI CCANNNNNNNNNTGG 1 cut(s) 315
XmiI GTMKAC 4 cut(s) 271, 1553, 1584, 2104
XmnI GAANNNNTTC 5 cut(s) 128, 1037, 1116, 1495, 2247
Zsp2I ATGCAT 2 cut(s) 673, 1527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.