Rw5G032280

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000110
Physical Location & Seq
Reverse (-)
54497309 .. 54500092
2784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G032280.1

Sequence Viewer

Length: 2784 bp
ATGGCCTTGTCTGTAACGGACCTCTTAATTGGAAAACTTGCGACCATTCTTGAGTACGAAGGAACAACCATAGCAGGTGTTCGTCATGAAGTTGATAAGATTAAGGAGGAGTTTCTAAGCATGAAAGCTTTCCTACTGGATTCGGAGGCCAACAAACCAAAAACTGAAGGACAGAAATTGTGGGTTGCAAGAATCAGAGACTTAGCCTATGATGTTGAAGATATCATTGATGAATTCATGTATCACATGTATAAGAAGCAAAGTGGGGGTCGACTTTCAAGGGGGCTACAAAAAACCATTCGCGCTCCATGTAATATTTGGTTCAGGCATAAAATTGCAAAGAAGTTGCGGAAAATTACTGACATGATCAAAGCCATTTCAGAGAGGAATAAAAGATACGGTGTTGGCCTTGTAGGAGGATCAACTACATCCGAAGATATTCAGAAATTGGTGCAGAACCAAGCGGAGTCTTCATTTTACATTATGGAAGACGAATTGGTTGGGATTGAAGGCAAGAAGCAGATACTAATGGGATGGCTAATGGATGACGAGCAACACCAAACTGTTATATCTGTGGTGGGCACGGGAGGTTCTGGAAAGACAACTCTAGTTGCCAAGACCTTCACCAATGAACTGGTAAAGAGACATTTTGATTGTTATGCATGGATTACTGTTTCCCAAACTTATGATGTGGTACACTTGTTTCGAAGCTTGATCAGGGAACTCTACAAATCAAGGAAGGAAGATTTCCCTGCAAATTTGAATGCCATGAGCCGCATAGAATTGCTAGAGTTACTAGTTAACTACTTGGAGTCCAAAAAATACCTAGTTGTTCTAGATGACGTGTGGGAGTTAAGAGATTGGAGAGAAATAAAGGTATCACTTCAAGATAGACAACTTGGAAGTCGAGTCGTACTTACAACTCGAAATGAAGAAGCAGCATCCAACTCTTTTGGAGTTGAAAGCTATGTTTACCGTATTCAGCCCCTGCAAAAGAATGAGGCTTGGGAACTCTTCAGCAGGAAAGCATTCTCAATTAAGCAACACAAAACTTGTCCAACTGAGCTGAAGTCATTAGCTTGCCAACTTGTGGAGAAGTGTGAAGGCCTTCCTCTCGGTATTGTGGCTTTAGGTGGTCTTATGTGTTCTAAGAAGTCGCTGGATCAATGGCAACAAGTCTGCAACAACTTGAATTGGTATCTACATAACAATTCATCGCTAGACCCTGTGAGAAACATTTTGTTGCTGAGTTTCAATGATTTGCCATCTCAACTGAAGCATTGCTTCCTCTATTGTTCCCTTTTTCCAGAAGATTATCTATTTAAAAGAAAAAGGCTGATTAGATTGTGGATAGCAGAAGGATTTGTTGAAAATGTGAAAGGGGTCACACCCGAAGAAGTTGCAGATGGCTATCTTTGGGAACTCTACTCCCGTAGCATGTTACAAGTTGCAAGTGGGTTTGTTTCCGGAGCACCTTTGGGAAGGCCGAAAAAATTTAAGATGCATGATCTGATGCGAGAGCTTGCTTTGTCGACAGCCGAAAAAGAAAAGTTTGGTTATGTATACGACGGGAGAGAAGTAACGGAAGAGATCTCAACCCGTCGCTTGTCAATTCACAGAATGAAAGGAGAAATTAAATCATGGAGGGATGTGTCAAAGATTCGTTCTCTTCTTGTATTTGACACTGACATGTTCTCATTGTCTTTCTTAAATACACTGGTTTCTCGATTCAAATTGTTGAGGACTCTAGACTTGGAGGATGTCCAAATTGATAAACTGCCCGTTGCAGTTTTCTACTCGTTCAACTTGAGATATTTAAACTTGAGGGGCACTTTAATCGAGGAACTTCCAGAGTCCATAAGGCATCTCCGCAACCTTCAATTTCTGAACATCAGGAATACTAAGATAGAGTCACTTCCACAAGGAATTACGAGGTTGCTGAATCTGCGCAATCTAATTATGTTTCGCTACATTGGGAACCTGTACTTCAAAGTTGGGCCCTTCAAAGTTGGGAGTGGGACAAAAGTGCCATCAAATATTTGTAAGTTGCAGAAATTGCAAAGTATATCAATGATTGAATCTGAAGGGGACACTAGTAGACTCCTCGCGATCGGTAACATGACTCAACTTAGGAGCATCAGCATATCGAATCTGAAAGAAAGAGATGAGATTGACCTTTGGGTCTCAATTCAAAAGTTGACTCTGCTTCAATCTATGGGTTTAATGGCTAGTAATGAAGAGGAAATTCTTCCGGTGAAAGCAGAATGTCCACCTCTTCCGCACCTTCGAAAGCTTGCTTTGGCTGGGAGACTGCAAAATGTAATACCTTGGTTTTCTTCACTGCATAGCCTCACAACTCTGATTCTGCATTGGTCAAGACTTGAAGAGGATTTACTACCTCAAATTGAAGCACTGCTCAATCTAACAAGGCTTAATCTTTGTAATGCATATGTTGGGGAAGAGTTGTGTTTCCGCAGGGGCTTTGTAAAGCTGATGAGCTTGGAGTTGTTGAATTTTGCCGCGGTGAAAAAGATAACTACAGAAAAAGGGGTGATGCCAAATCTCCAGTACTTATGGGTTAACGGCTTCATGGAGTTGAAAACAGTGCCATTGGGCCTGGAATACCTTTCTACTCTACAATATTTGGGCCTAAAAGATGTTCCCATAGAAGTTATAACGCATATTGGGAAAGGAGGTGTGGATCGCTATAAGCTACAACACATTCCTGAGATCATACATGTTTACGAACAGTCATCTAAGTGGATCCACGAAAACTTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

927

Amino Acids

106.66

Weight (kDa)

8.9

Isoelectric Point (pI)

50.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 90 7.2e-22 Rx N-terminal domain
NB-ARC PF00931 172 - 346 8.7e-45 NB-ARC domain
WHD_DRP PF23559 434 - 509 3.7e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 553 - 881 1.5e-35 Leucine-rich repeat region
LRR_8 PF13855 580 - 636 4e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2675
AarI CACCTGC 1 cut(s) 65
AasI GACNNNNNNGTC 1 cut(s) 2180
Acc16I TGCGCA 1 cut(s) 1949
Acc36I ACCTGC 1 cut(s) 65
AccB7I CCANNNNNTGG 1 cut(s) 1090
AccI GTMKAC 4 cut(s) 271, 1532, 1563, 2098
AccII CGCG 3 cut(s) 303, 2108, 2522
AccIII TCCGGA 1 cut(s) 1466
AciI CCGC 8 cut(s) 349, 464, 775, 1870, 2279, 2473, 2520, 2522
AclWI GGATC 5 cut(s) 427, 1170, 2709, 2758, 2771
AcsI RAATTY 5 cut(s) 233, 757, 1493, 2244, 2512
AcuI CTGAAG 5 cut(s) 186, 1000, 1088, 1295, 2103
AfaI GTAC 5 cut(s) 56, 696, 915, 1985, 2570
AfiI CCNNNNNNNGG 1 cut(s) 1090
AflIII ACRYGT 4 cut(s) 246, 843, 1689, 2737
AhlI ACTAGT 2 cut(s) 796, 2093
AjiI CACGTC 1 cut(s) 844
AjnI CCWGG 1 cut(s) 2616
Alw21I GWGCWC 1 cut(s) 1474
Alw26I GTCTC 4 cut(s) 192, 637, 2188, 2302
AlwI GGATC 5 cut(s) 427, 1170, 2709, 2758, 2771
AlwNI CAGNNNCTG 1 cut(s) 988
Aor13HI TCCGGA 1 cut(s) 1466
AoxI GGCC 9 cut(s) 3, 147, 406, 1105, 1484, 1997, 2614, 2647, 2778
ApaI GGGCCC 1 cut(s) 2001
ApeKI GCWGC 1 cut(s) 938
ApoI RAATTY 5 cut(s) 233, 757, 1493, 2244, 2512
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2247
AspLEI GCGC 2 cut(s) 305, 1950
AspS9I GGNCC 5 cut(s) 19, 1997, 1998, 2614, 2647
AsuHPI GGTGA 4 cut(s) 616, 2266, 2536, 2563
AsuII TTCGAA 2 cut(s) 706, 2287
AvaII GGWCC 1 cut(s) 19
BaeGI GKGCMC 3 cut(s) 584, 1832, 2001
BaeI ACNNNNGTAYC 2 cut(s) 686, 719
BamHI GGATCC 1 cut(s) 2763
BanII GRGCYC 1 cut(s) 2001
BarI GAAGNNNNNNTAC 2 cut(s) 2376, 2408
BbsI GAAGAC 2 cut(s) 462, 495
Bbv12I GWGCWC 1 cut(s) 1474
BbvI GCAGC 1 cut(s) 950
BccI CCATC 4 cut(s) 528, 1273, 1400, 2038
BceAI ACGGC 1 cut(s) 2599
BcgI CGANNNNNNTGC 4 cut(s) 1382, 1416, 1819, 1853
BciT130I CCWGG 1 cut(s) 2618
BclI TGATCA 2 cut(s) 366, 714
BcoDI GTCTC 4 cut(s) 192, 637, 2188, 2302
BcuI ACTAGT 2 cut(s) 796, 2093
BfmI CTRYAG 1 cut(s) 2538
BfuAI ACCTGC 1 cut(s) 65
BglII AGATCT 1 cut(s) 1590
BisI GCNGC 3 cut(s) 775, 939, 2520
BlsI GCNGC 3 cut(s) 776, 940, 2521
BmcAI AGTACT 1 cut(s) 2570
Bme1390I CCNGG 1 cut(s) 2618
Bme18I GGWCC 1 cut(s) 19
BmgBI CACGTC 1 cut(s) 844
BmgT120I GGNCC 5 cut(s) 19, 1997, 1998, 2614, 2647
BmiI GGNNCC 3 cut(s) 1979, 1999, 2765
BmrFI CCNGG 1 cut(s) 2618
BmsI GCATC 6 cut(s) 950, 1491, 1503, 1873, 2145, 2544
BpiI GAAGAC 2 cut(s) 462, 495
BpmI CTGGAG 1 cut(s) 2549
Bpu14I TTCGAA 2 cut(s) 706, 2287
BpuEI CTTGAG 3 cut(s) 71, 1828, 1843
BsaBI GATNNNNATC 1 cut(s) 1410
BsaI GGTCTC 1 cut(s) 2188
BsaJI CCNNGG 2 cut(s) 2327, 2520
BsaWI WCCGGW 2 cut(s) 1466, 2251
Bsc4I CCNNNNNNNGG 1 cut(s) 1090
Bse1I ACTGG 4 cut(s) 141, 639, 1722, 2566
Bse3DI GCAATG 1 cut(s) 1279
Bse8I GATNNNNATC 1 cut(s) 1410
BseAI TCCGGA 1 cut(s) 1466
BseBI CCWGG 1 cut(s) 2618
BseDI CCNNGG 2 cut(s) 2327, 2520
BseGI GGATG 6 cut(s) 428, 539, 550, 941, 1654, 1765
BseJI GATNNNNATC 1 cut(s) 1410
BseLI CCNNNNNNNGG 1 cut(s) 1090
BseMI GCAATG 1 cut(s) 1279
BseMII CTCAG 3 cut(s) 1053, 1238, 2718
BseNI ACTGG 4 cut(s) 141, 639, 1722, 2566
BseRI GAGGAG 2 cut(s) 122, 2093
BseSI GKGCMC 3 cut(s) 584, 1832, 2001
BseXI GCAGC 1 cut(s) 950
BseYI CCCAGC 1 cut(s) 2303
BsgI GTGCAG 1 cut(s) 473
Bsh1236I CGCG 3 cut(s) 303, 2108, 2522
Bsh1285I CGRYCG 1 cut(s) 2112
BshFI GGCC 9 cut(s) 5, 149, 408, 1107, 1486, 1999, 2616, 2649, 2780
BsiEI CGRYCG 1 cut(s) 2112
BsiHKAI GWGCWC 1 cut(s) 1474
BsiSI CCGG 2 cut(s) 1467, 2252
BslFI GGGAC 2 cut(s) 2032, 2102
BslI CCNNNNNNNGG 1 cut(s) 1090
BsmAI GTCTC 4 cut(s) 192, 637, 2188, 2302
BsmFI GGGAC 2 cut(s) 2032, 2102
BsmI GAATGC 2 cut(s) 769, 1028
BsnI GGCC 9 cut(s) 5, 149, 408, 1107, 1486, 1999, 2616, 2649, 2780
Bso31I GGTCTC 1 cut(s) 2188
Bsp119I TTCGAA 2 cut(s) 706, 2287
Bsp120I GGGCCC 1 cut(s) 1997
Bsp1286I GDGCHC 4 cut(s) 584, 1474, 1832, 2001
Bsp13I TCCGGA 1 cut(s) 1466
Bsp68I TCGCGA 1 cut(s) 2108
BspACI CCGC 8 cut(s) 349, 464, 775, 1870, 2279, 2473, 2520, 2522
BspANI GGCC 9 cut(s) 5, 149, 408, 1107, 1486, 1999, 2616, 2649, 2780
BspCNI CTCAG 3 cut(s) 1054, 1239, 2719
BspEI TCCGGA 1 cut(s) 1466
BspFNI CGCG 3 cut(s) 303, 2108, 2522
BspHI TCATGA 1 cut(s) 85
BspLI GGNNCC 3 cut(s) 1979, 1999, 2765
BspMI ACCTGC 1 cut(s) 65
BspPI GGATC 5 cut(s) 427, 1170, 2709, 2758, 2771
BspT104I TTCGAA 2 cut(s) 706, 2287
BspTNI GGTCTC 1 cut(s) 2188
BsrDI GCAATG 1 cut(s) 1279
BsrI ACTGG 4 cut(s) 141, 639, 1722, 2566
BssECI CCNNGG 2 cut(s) 2327, 2520
BssNAI GTATAC 1 cut(s) 1564
BssT1I CCWWGG 1 cut(s) 2327
Bst1107I GTATAC 1 cut(s) 1564
Bst2UI CCWGG 1 cut(s) 2618
Bst4CI ACNGT 6 cut(s) 401, 565, 673, 977, 2605, 2751
Bst6I CTCTTC 7 cut(s) 1019, 1581, 1674, 2232, 2280, 2379, 2454
BstAPI GCANNNNNTGC 1 cut(s) 2056
BstBI TTCGAA 2 cut(s) 706, 2287
BstC8I GCNNGC 3 cut(s) 1081, 1524, 2295
BstDEI CTNAG 9 cut(s) 116, 202, 1062, 1149, 1247, 1902, 2129, 2727, 2757
BstDSI CCRYGG 1 cut(s) 2520
BstF5I GGATG 6 cut(s) 428, 539, 550, 941, 1654, 1765
BstFNI CGCG 3 cut(s) 303, 2108, 2522
BstHHI GCGC 2 cut(s) 305, 1950
BstMAI GTCTC 4 cut(s) 192, 637, 2188, 2302
BstMCI CGRYCG 1 cut(s) 2112
BstMWI GCNNNNNNNGC 2 cut(s) 1945, 2056
BstNI CCWGG 1 cut(s) 2618
BstNSI RCATGY 4 cut(s) 250, 1441, 1693, 2741
BstSCI CCNGG 1 cut(s) 2616
BstSFI CTRYAG 1 cut(s) 2538
BstSLI GKGCMC 3 cut(s) 584, 1832, 2001
BstUI CGCG 3 cut(s) 303, 2108, 2522
BstV1I GCAGC 1 cut(s) 950
BstV2I GAAGAC 2 cut(s) 462, 495
BstX2I RGATCY 2 cut(s) 1590, 2763
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 2 cut(s) 1590, 2763
BstZ17I GTATAC 1 cut(s) 1564
BsuRI GGCC 9 cut(s) 5, 149, 408, 1107, 1486, 1999, 2616, 2649, 2780
BtgI CCRYGG 1 cut(s) 2520
BtgZI GCGATG 1 cut(s) 1200
BtrI CACGTC 1 cut(s) 844
BtsCI GGATG 6 cut(s) 428, 539, 550, 941, 1654, 1765
BtsI GCAGTG 2 cut(s) 2339, 2411
BtsIMutI CAGTG 5 cut(s) 1683, 1715, 2339, 2411, 2610
BtuMI TCGCGA 1 cut(s) 2108
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 3 cut(s) 1081, 1524, 2295
CaiI CAGNNNCTG 1 cut(s) 988
CciI TCATGA 1 cut(s) 85
CfoI GCGC 2 cut(s) 305, 1950
Cfr13I GGNCC 5 cut(s) 19, 1997, 1998, 2614, 2647
Cfr42I CCGCGG 1 cut(s) 2523
Csp6I GTAC 5 cut(s) 55, 695, 914, 1984, 2569
CviQI GTAC 5 cut(s) 55, 695, 914, 1984, 2569
DdeI CTNAG 9 cut(s) 116, 202, 1062, 1149, 1247, 1902, 2129, 2727, 2757
DraI TTTAAA 2 cut(s) 1324, 1818
DrdI GACNNNNNNGTC 1 cut(s) 2180
DseDI GACNNNNNNGTC 1 cut(s) 2180
Eam1104I CTCTTC 7 cut(s) 1019, 1581, 1674, 2232, 2280, 2379, 2454
EarI CTCTTC 7 cut(s) 1019, 1581, 1674, 2232, 2280, 2379, 2454
Eco130I CCWWGG 1 cut(s) 2327
Eco147I AGGCCT 1 cut(s) 1107
Eco24I GRGCYC 1 cut(s) 2001
Eco31I GGTCTC 1 cut(s) 2188
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 19
Eco57I CTGAAG 5 cut(s) 186, 1000, 1088, 1295, 2103
EcoO109I RGGNCCY 1 cut(s) 1998
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 2616
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 2327
EcoT22I ATGCAT 3 cut(s) 664, 1506, 2449
EcoT38I GRGCYC 1 cut(s) 2001
ErhI CCWWGG 1 cut(s) 2327
FalI AAGNNNNNCTT 2 cut(s) 1268, 1300
FaqI GGGAC 2 cut(s) 2032, 2102
FauNDI CATATG 1 cut(s) 2449
FbaI TGATCA 2 cut(s) 366, 714
FblI GTMKAC 4 cut(s) 271, 1532, 1563, 2098
Fnu4HI GCNGC 3 cut(s) 775, 939, 2520
FokI GGATG 6 cut(s) 415, 546, 557, 928, 1661, 1772
FriOI GRGCYC 1 cut(s) 2001
Fsp4HI GCNGC 3 cut(s) 775, 939, 2520
FspI TGCGCA 1 cut(s) 1949
GlaI GCGC 2 cut(s) 304, 1949
GluI GCNGC 3 cut(s) 775, 939, 2520
GsaI CCCAGC 1 cut(s) 2307
GsuI CTGGAG 1 cut(s) 2549
HaeIII GGCC 9 cut(s) 5, 149, 408, 1107, 1486, 1999, 2616, 2649, 2780
HapII CCGG 2 cut(s) 1467, 2252
HhaI GCGC 2 cut(s) 305, 1950
Hin6I GCGC 2 cut(s) 303, 1948
HinP1I GCGC 2 cut(s) 303, 1948
HincII GTYRAC 5 cut(s) 272, 802, 1533, 2199, 2581
HindII GTYRAC 5 cut(s) 272, 802, 1533, 2199, 2581
HindIII AAGCTT 3 cut(s) 126, 709, 2291
HpaI GTTAAC 2 cut(s) 802, 2581
HpaII CCGG 2 cut(s) 1467, 2252
HphI GGTGA 4 cut(s) 616, 2266, 2536, 2563
Hpy99I CGWCG 2 cut(s) 1571, 1605
HpyCH4III ACNGT 6 cut(s) 401, 565, 673, 977, 2605, 2751
HpyCH4IV ACGT 1 cut(s) 843
HpyF10VI GCNNNNNNNGC 2 cut(s) 1945, 2056
HpyF3I CTNAG 9 cut(s) 116, 202, 1062, 1149, 1247, 1902, 2129, 2727, 2757
HpySE526I ACGT 1 cut(s) 843
HspAI GCGC 2 cut(s) 303, 1948
Kpn2I TCCGGA 1 cut(s) 1466
Ksp22I TGATCA 2 cut(s) 366, 714
KspAI GTTAAC 2 cut(s) 802, 2581
KspI CCGCGG 1 cut(s) 2523
LmnI GCTCC 3 cut(s) 310, 1469, 2133
Lsp1109I GCAGC 1 cut(s) 950
LweI GCATC 6 cut(s) 950, 1491, 1503, 1873, 2145, 2544
MaeII ACGT 1 cut(s) 843
MaeIII GTNAC 7 cut(s) 13, 792, 1384, 1440, 1579, 1911, 2114
MflI RGATCY 2 cut(s) 1590, 2763
MhlI GDGCHC 4 cut(s) 584, 1474, 1832, 2001
MlyI GAGTC 9 cut(s) 476, 821, 918, 1738, 1862, 1919, 2094, 2116, 2194
MmeI TCCRAC 2 cut(s) 969, 1082
Mph1103I ATGCAT 3 cut(s) 664, 1506, 2449
MroI TCCGGA 1 cut(s) 1466
MroXI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2247
MslI CAYNNNNRTG 2 cut(s) 1688, 2758
MspA1I CMGCKG 1 cut(s) 2522
MspI CCGG 2 cut(s) 1467, 2252
MspR9I CCNGG 1 cut(s) 2618
Mva1269I GAATGC 2 cut(s) 769, 1028
MvaI CCWGG 1 cut(s) 2618
MvnI CGCG 3 cut(s) 303, 2108, 2522
MwoI GCNNNNNNNGC 2 cut(s) 1945, 2056
NdeI CATATG 1 cut(s) 2449
NlaIV GGNNCC 3 cut(s) 1979, 1999, 2765
NmuCI GTSAC 2 cut(s) 1384, 1911
NruI TCGCGA 1 cut(s) 2108
NsbI TGCGCA 1 cut(s) 1949
NsiI ATGCAT 3 cut(s) 664, 1506, 2449
NspI RCATGY 4 cut(s) 250, 1441, 1693, 2741
NspV TTCGAA 2 cut(s) 706, 2287
PagI TCATGA 1 cut(s) 85
PaqCI CACCTGC 1 cut(s) 65
PceI AGGCCT 1 cut(s) 1107
PciI ACATGT 3 cut(s) 246, 1689, 2737
PctI GAATGC 2 cut(s) 769, 1028
PdmI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2247
PfeI GAWTC 8 cut(s) 140, 192, 1660, 1728, 1942, 2078, 2149, 2362
PflMI CCANNNNNTGG 1 cut(s) 1090
PkrI GCNGC 3 cut(s) 776, 940, 2521
Ple19I CGATCG 1 cut(s) 2112
PleI GAGTC 9 cut(s) 475, 820, 917, 1738, 1861, 1918, 2094, 2116, 2194
PpsI GAGTC 9 cut(s) 475, 820, 917, 1738, 1861, 1918, 2094, 2116, 2194
PscI ACATGT 3 cut(s) 246, 1689, 2737
PsiI TTATAA 1 cut(s) 2675
Psp6I CCWGG 1 cut(s) 2616
PspFI CCCAGC 1 cut(s) 2303
PspGI CCWGG 1 cut(s) 2616
PspN4I GGNNCC 3 cut(s) 1979, 1999, 2765
PspOMI GGGCCC 1 cut(s) 1997
PspPI GGNCC 5 cut(s) 19, 1997, 1998, 2614, 2647
PstNI CAGNNNCTG 1 cut(s) 988
PsuI RGATCY 2 cut(s) 1590, 2763
PvuI CGATCG 1 cut(s) 2112
RruI TCGCGA 1 cut(s) 2108
RsaI GTAC 5 cut(s) 56, 696, 915, 1985, 2570
RsaNI GTAC 5 cut(s) 55, 695, 914, 1984, 2569
RseI CAYNNNNRTG 2 cut(s) 1688, 2758
SacII CCGCGG 1 cut(s) 2523
SalI GTCGAC 2 cut(s) 270, 1531
SatI GCNGC 3 cut(s) 775, 939, 2520
Sau96I GGNCC 5 cut(s) 19, 1997, 1998, 2614, 2647
ScaI AGTACT 1 cut(s) 2570
SchI GAGTC 9 cut(s) 476, 821, 918, 1738, 1862, 1919, 2094, 2116, 2194
ScrFI CCNGG 1 cut(s) 2618
SduI GDGCHC 4 cut(s) 584, 1474, 1832, 2001
SfaNI GCATC 6 cut(s) 950, 1491, 1503, 1873, 2145, 2544
SfcI CTRYAG 1 cut(s) 2538
Sfr303I CCGCGG 1 cut(s) 2523
SfuI TTCGAA 2 cut(s) 706, 2287
SgrBI CCGCGG 1 cut(s) 2523
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 2 cut(s) 1688, 2758
SmlI CTYRAG 3 cut(s) 50, 1807, 1822
SmoI CTYRAG 3 cut(s) 50, 1807, 1822
SpeI ACTAGT 2 cut(s) 796, 2093
SseBI AGGCCT 1 cut(s) 1107
SsiI CCGC 8 cut(s) 349, 464, 775, 1870, 2279, 2473, 2520, 2522
SspI AATATT 3 cut(s) 316, 2038, 2642
StuI AGGCCT 1 cut(s) 1107
StyD4I CCNGG 1 cut(s) 2616
StyI CCWWGG 1 cut(s) 2327
TaaI ACNGT 6 cut(s) 401, 565, 673, 977, 2605, 2751
TaiI ACGT 1 cut(s) 846
TaqI TCGA 9 cut(s) 271, 706, 907, 925, 1532, 1726, 1839, 2147, 2287
TatI WGTACW 2 cut(s) 1983, 2568
TauI GCSGC 2 cut(s) 777, 2522
TfiI GAWTC 8 cut(s) 140, 192, 1660, 1728, 1942, 2078, 2149, 2362
TscAI CASTG 5 cut(s) 1690, 1722, 2346, 2418, 2610
TseFI GTSAC 2 cut(s) 1384, 1911
TseI GCWGC 1 cut(s) 938
Tsp45I GTSAC 2 cut(s) 1384, 1911
TspGWI ACGGA 2 cut(s) 32, 1598
TspRI CASTG 5 cut(s) 1690, 1722, 2346, 2418, 2610
Van91I CCANNNNNTGG 1 cut(s) 1090
VpaK11BI GGWCC 1 cut(s) 19
XapI RAATTY 5 cut(s) 233, 757, 1493, 2244, 2512
XbaI TCTAGA 2 cut(s) 835, 1747
XceI RCATGY 4 cut(s) 250, 1441, 1693, 2741
XcmI CCANNNNNNNNNTGG 1 cut(s) 315
XmiI GTMKAC 4 cut(s) 271, 1532, 1563, 2098
XmnI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2247
ZrmI AGTACT 1 cut(s) 2570
Zsp2I ATGCAT 3 cut(s) 664, 1506, 2449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.