RchiOBHm_Chr1g0348041

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000110
Physical Location & Seq
Reverse (-)
40763497 .. 40766310
2814 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57410

Sequence Viewer

Length: 2814 bp
ATGGCCTTGCCGACGACAGATTTGTTGATTGGAAAAATTGTAGCTATTGTTGAGAATGAAGCATTATCCATATCCAGTGTTCGTGATGAAATCGATGAGATCAAGCAGGAGCTGGTTAGCATGAAATCTTTCCTGCAGGATGCTGATGGAAAGAAAGAAAACAGCGAAGGAGAGGAAACGTGGGTCGCAAGCGTCAGAGACTTGGCCTATGATGTTGAGGACATAATTGATGAATTCATGTACCACATATATGAGAAGCAAACTGGTGGCCTATTTCCAAGGTGGCTCCACAAGATCATTCACATTCCAAGGAGTCTCTGGTATAAACGTCAAATTGCCAACAAATTGCAGAAAACCACTAAAATGATCAAAGCCATTCCAGAGAGGAATCAGAGATATGCTATTGGTGGTGTAGAAGGGACAACAAGTTCAATTGATGTTGACAAATGGGTGCAGAACCAAGCAGAGTCTTCTCTTTTTATTAAGGAAGATGAACTTGTAGGCATTGAAGGCAACAAGCAAACAATAATGGGATGGCTGATGGATGAAGAGCAACATCAAACTATTGTCTCTGTGGTCGGGATGGGAGGTTCAGGAAAGACAACTCTAGTTGCCAAGACCTTCACCAATGAAAGTGTGAAAAGACATTTTGACTGCTATGCGTGGATTACTGTTTCCAGAACCTATGCCATTGAAGACTTATTTAGATGCTTGATCAAGGAGTTCCACCAATCAAGAAAAGAAAAGGTTCCTGCACCTGCAAAACTGAAGGACATGAGCTACAGAGAATTGCTAGAGAAGCTCGTGAACTACTTGGAGAGTAAAAGGTACCTAGTTGTATTGGATGACGTGTGGGATGTTAAACTTTGGACAGAAATAAATATATCACTCCCTGATAGACGACTTGGAAGTCGGATCCTTCTTACAACTCGGAAAGAAGACATAGCACATTATTCTTTCGGAGTGGTAAGCCATGTTCATCTTATTAAGCCCTTGGACAACAATGAGGCGTGGGAGCTTTTCAGCAAGAAAGCATTCTCAACTTACCATAATAGATTTTGTACACCGGAGCTTGAACCGTTAGCTTCAAAACTTGTGGAGAAGTGTAAAGGTTTGCCTCTTGCAATTGTTGCTTTAGGAGGTCTTATGTCTTCCAAAAAGTCAACCACAGAGTGGAGGGATGTCTACAACAGCTTAAATTGGCATCTAACTAACAATCCCTTGTTAGATAATGTGAAGAGCATTTTGTTGCTCAGTTACAATGATTTGCCTTTCCGACTCAAGCAATGTTTCTTGTATTGTTCTCTTTTCCCAGAAGATTATTTGATTCGAAGAAAAAGGCTCTTCAGGTTGTGGATATCTGAAGGACTTGTTCAACATGTCCAAGGGGTCACTCCGCAAGCAGCTACAGAAAACTATCTTATGGAACTCATTGTTCGTAGCATGCTACAAGTTGTAAGGAGGAATGAAACTGGGAGGCCAAAGGCATTTAAGATGCATGATCTTATGCGGGAGCTTGCTCTATCAATGTCAACAAGAGAAAAGTTTTTTGTTGTATATGATGGGAGAGAATCAGTAATGGAAGAAATTGGAGCCCGGCGTTTGTCAATTCAAAGCACTGAAGGAACAGAAATCAAATCATGGATAGGTATGTCAAAGCTTCGTACCCTTCTTGTGTTTGTCACTGGCATAATACCATTATCTTTCTCAAACGCATTGCCTTCTGGATTTAAATTATTAAGGGTTCTAGATGTGGAGGATATGCCACTTGATAAACTGCCAAATGATCTAGTGTACTTATTCAACTTGAGATATTTGAATTTGAAAGGAACTGCAATTAGGGAGCTTCCAAAATCCATAGGGTATCTCCATAACCTTCACTTCTTGGACATTAGGAAAACAAAGATTGAGGCACTTCCAAAAGGATTTTCTAAGTTGCTAAACTTGCGCCACCTAATGATGTATTATAATAATAAGGATTATAATGATTTCAAGATTGTAAGTGGGACAAGAGCAACTTCAAATATCAGCAAGTTGAAGAAATTGCAAGTTTTGTCTAGAGTTCAATCCGAAGGGAATATCATTAGACTACTCGGGAACATGACCCAGCTTACAAGGATTGGCATAACCAACCTAACTGAGAGAGATGAGATGGACCTATGTGACTCAATCCAAAAGATGAAACTCCTTACCAATTTGAGCTTGATGGTAAAGGATGAAGAAGAATTTCTTCGAGCTGATAATGCACTGTTTTCACCTCCTCCTCACCTTAGAAAGCTTTGTTTCATTGGGAAACTGGAAAAAGTACCACATTGGTTTTCTTCACTCGACAGCCTCACATCCATGCATCTGCATTGGCCTAGACTTGAGGAAGATTTATTATCTCACATCGAAGGACTGCCGTTTTTGGGAAGTCTTACACTTGTTAATGCATATGTCGGGAGTGAGTTACGTTTCAGTAGAGGCTTTGTGAAGCTTTCAAATTTGCAATTATTTAAATTCCCCTCATTGAGTAAGATAACAGTGGAAAAGGGGGTGATGCCAAATCTCCAGTTCCTAACACTCAATAGCTGCATGGAATTAAAGAGATTGCCTCATGGTCTTGAACACCTTACTAATCTACAAACATTGAGCTTGGAGTTTCTTTCGAGCCAACTTATACAGTCCATACGAGAAGGAGGTGTGGATCGTCCAAAGGTACAACATATCCTAGAAATCAACCATTTTTTCGACTCTTCAGGTGGGAGGTCTTGCGAAAGCTTGTCTCCAAGGGCTCTTTACCGAGGAGGTAATAAACTTGCTTTGTATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

937

Amino Acids

107.75

Weight (kDa)

8.88

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 87 1.6e-22 Rx N-terminal domain
NB-ARC PF00931 170 - 347 2.6e-48 NB-ARC domain
WHD_DRP PF23559 436 - 507 2.8e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 573 - 883 7.4e-44 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1968, 1983
AarI CACCTGC 1 cut(s) 766
AasI GACNNNNNNGTC 1 cut(s) 909
Acc36I ACCTGC 1 cut(s) 766
Acc65I GGTACC 1 cut(s) 828
AccB1I GGYRCC 1 cut(s) 828
AccB7I CCANNNNNTGG 1 cut(s) 1173
AccI GTMKAC 1 cut(s) 1185
AciI CCGC 2 cut(s) 1397, 1510
AclWI GGATC 3 cut(s) 910, 923, 2694
AcsI RAATTY 5 cut(s) 233, 1819, 2225, 2482, 2498
AcuI CTGAAG 5 cut(s) 788, 1330, 1383, 1641, 2721
AdeI CACNNNGTG 1 cut(s) 1173
AfaI GTAC 7 cut(s) 242, 830, 1063, 1666, 1796, 2307, 2700
AfiI CCNNNNNNNGG 2 cut(s) 1173, 2408
AflIII ACRYGT 2 cut(s) 849, 1378
AjiI CACGTC 1 cut(s) 850
AjuI GAANNNNNNNTTGG 2 cut(s) 1912, 1944
AloI GAACNNNNNNTCC 4 cut(s) 412, 444, 574, 606
Alw26I GTCTC 4 cut(s) 192, 320, 574, 2769
AlwI GGATC 3 cut(s) 910, 923, 2694
AlwNI CAGNNNCTG 1 cut(s) 112
Ama87I CYCGRG 1 cut(s) 2093
AoxI GGCC 5 cut(s) 3, 204, 268, 1478, 2357
ApeKI GCWGC 2 cut(s) 1403, 2571
ApoI RAATTY 5 cut(s) 233, 1819, 2225, 2482, 2498
ArsI GACNNNNNNTTYG 1 cut(s) 36
Asp700I GAANNNNTTC 5 cut(s) 128, 747, 1034, 2226, 2229
Asp718I GGTACC 1 cut(s) 828
AspLEI GCGC 1 cut(s) 1950
AspS9I GGNCC 1 cut(s) 2155
AsuC2I CCSGG 1 cut(s) 1597
AsuHPI GGTGA 4 cut(s) 616, 2247, 2258, 2548
AsuII TTCGAA 1 cut(s) 1330
AvaI CYCGRG 1 cut(s) 2093
AvaII GGWCC 1 cut(s) 2155
BamHI GGATCC 1 cut(s) 915
BanI GGYRCC 1 cut(s) 828
BanII GRGCYC 2 cut(s) 1597, 2776
BauI CACGAG 1 cut(s) 803
BbsI GAAGAC 4 cut(s) 462, 702, 945, 1143
BbvI GCAGC 2 cut(s) 1415, 2558
BccI CCATC 7 cut(s) 140, 528, 535, 577, 1556, 2146, 2200
BceAI ACGGC 1 cut(s) 2386
BclI TGATCA 2 cut(s) 366, 714
BcnI CCSGG 1 cut(s) 1597
BcoDI GTCTC 4 cut(s) 192, 320, 574, 2769
BfaI CTAG 8 cut(s) 608, 794, 833, 1748, 1790, 2058, 2361, 2711
BfmI CTRYAG 3 cut(s) 134, 781, 1407
BfuAI ACCTGC 1 cut(s) 766
BisI GCNGC 2 cut(s) 1404, 2572
BlsI GCNGC 2 cut(s) 1405, 2573
Bme1390I CCNGG 1 cut(s) 1597
Bme18I GGWCC 1 cut(s) 2155
BmeT110I CYCGRG 1 cut(s) 2093
BmgBI CACGTC 1 cut(s) 850
BmgT120I GGNCC 1 cut(s) 2155
BmiI GGNNCC 5 cut(s) 287, 750, 830, 917, 1594
BmrFI CCNGG 1 cut(s) 1597
BmrI ACTGGG 1 cut(s) 1482
BmsI GCATC 6 cut(s) 130, 698, 1213, 1485, 2356, 2529
BmuI ACTGGG 1 cut(s) 1482
BpiI GAAGAC 4 cut(s) 462, 702, 945, 1143
BplI GAGNNNNNCTC 2 cut(s) 2578, 2610
BpmI CTGGAG 1 cut(s) 2534
Bpu14I TTCGAA 1 cut(s) 1330
BpuEI CTTGAG 3 cut(s) 1265, 1828, 2387
BpuMI CCSGG 1 cut(s) 1597
Bsa29I ATCGAT 1 cut(s) 93
BsaJI CCNNGG 6 cut(s) 278, 308, 993, 1384, 2768, 2782
BsaWI WCCGGW 1 cut(s) 1066
Bsc4I CCNNNNNNNGG 2 cut(s) 1173, 2408
Bse1I ACTGG 6 cut(s) 75, 268, 1477, 1690, 2301, 2551
Bse3DI GCAATG 2 cut(s) 1292, 1715
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 6 cut(s) 278, 308, 993, 1384, 2768, 2782
BseGI GGATG 9 cut(s) 145, 539, 550, 588, 850, 862, 1186, 2221, 2339
BseLI CCNNNNNNNGG 2 cut(s) 1173, 2408
BseMI GCAATG 2 cut(s) 1292, 1715
BseMII CTCAG 2 cut(s) 1267, 2130
BseNI ACTGG 6 cut(s) 75, 268, 1477, 1690, 2301, 2551
BseRI GAGGAG 3 cut(s) 2250, 2253, 2799
BseXI GCAGC 2 cut(s) 1415, 2558
BseYI CCCAGC 1 cut(s) 2106
BsgI GTGCAG 2 cut(s) 473, 738
BshFI GGCC 5 cut(s) 5, 206, 270, 1480, 2359
BshNI GGYRCC 1 cut(s) 828
BshVI ATCGAT 1 cut(s) 93
BsiHKCI CYCGRG 1 cut(s) 2093
BsiSI CCGG 2 cut(s) 1067, 1597
BslFI GGGAC 2 cut(s) 433, 2020
BslI CCNNNNNNNGG 2 cut(s) 1173, 2408
BsmAI GTCTC 4 cut(s) 192, 320, 574, 2769
BsmFI GGGAC 2 cut(s) 433, 2020
BsmI GAATGC 1 cut(s) 1034
BsnI GGCC 5 cut(s) 5, 206, 270, 1480, 2359
BsoBI CYCGRG 1 cut(s) 2093
Bsp119I TTCGAA 1 cut(s) 1330
Bsp1286I GDGCHC 2 cut(s) 1597, 2776
Bsp1407I TGTACA 1 cut(s) 1061
Bsp143I GATC 8 cut(s) 99, 294, 366, 714, 915, 1501, 1786, 2686
BspACI CCGC 2 cut(s) 1397, 1510
BspANI GGCC 5 cut(s) 5, 206, 270, 1480, 2359
BspCNI CTCAG 2 cut(s) 1266, 2131
BspDI ATCGAT 1 cut(s) 93
BspLI GGNNCC 5 cut(s) 287, 750, 830, 917, 1594
BspMAI CTGCAG 1 cut(s) 138
BspMI ACCTGC 1 cut(s) 766
BspPI GGATC 3 cut(s) 910, 923, 2694
BspQI GCTCTTC 3 cut(s) 543, 1232, 1349
BspT104I TTCGAA 1 cut(s) 1330
BspT107I GGYRCC 1 cut(s) 828
BsrDI GCAATG 2 cut(s) 1292, 1715
BsrGI TGTACA 1 cut(s) 1061
BsrI ACTGG 6 cut(s) 75, 268, 1477, 1690, 2301, 2551
BssECI CCNNGG 6 cut(s) 278, 308, 993, 1384, 2768, 2782
BssMI GATC 8 cut(s) 99, 294, 366, 714, 915, 1501, 1786, 2686
BssSI CACGAG 1 cut(s) 803
BssT1I CCWWGG 5 cut(s) 278, 308, 993, 1384, 2768
Bst2BI CACGAG 1 cut(s) 803
Bst4CI ACNGT 5 cut(s) 673, 1080, 2250, 2524, 2664
Bst6I CTCTTC 4 cut(s) 543, 1232, 1349, 2740
BstAPI GCANNNNNTGC 1 cut(s) 1130
BstAUI TGTACA 1 cut(s) 1061
BstBI TTCGAA 1 cut(s) 1330
BstC8I GCNNGC 4 cut(s) 190, 1401, 1445, 1518
BstDEI CTNAG 4 cut(s) 1253, 1932, 2139, 2270
BstF5I GGATG 9 cut(s) 145, 539, 550, 588, 850, 862, 1186, 2221, 2339
BstHHI GCGC 1 cut(s) 1950
BstKTI GATC 8 cut(s) 102, 297, 369, 717, 918, 1504, 1789, 2689
BstMAI GTCTC 4 cut(s) 192, 320, 574, 2769
BstMBI GATC 8 cut(s) 99, 294, 366, 714, 915, 1501, 1786, 2686
BstMWI GCNNNNNNNGC 5 cut(s) 510, 799, 1130, 1945, 2243
BstNSI RCATGY 2 cut(s) 1382, 1447
BstSCI CCNGG 1 cut(s) 1595
BstSFI CTRYAG 3 cut(s) 134, 781, 1407
BstV1I GCAGC 2 cut(s) 1415, 2558
BstV2I GAAGAC 4 cut(s) 462, 702, 945, 1143
BstX2I RGATCY 1 cut(s) 915
BstYI RGATCY 1 cut(s) 915
Bsu15I ATCGAT 1 cut(s) 93
BsuRI GGCC 5 cut(s) 5, 206, 270, 1480, 2359
BsuTUI ATCGAT 1 cut(s) 93
BtrI CACGTC 1 cut(s) 850
BtsCI GGATG 9 cut(s) 145, 539, 550, 588, 850, 862, 1186, 2221, 2339
BtsIMutI CAGTG 5 cut(s) 82, 1617, 1683, 2246, 2529
BveI ACCTGC 1 cut(s) 766
Cac8I GCNNGC 4 cut(s) 190, 1401, 1445, 1518
CaiI CAGNNNCTG 1 cut(s) 112
CfoI GCGC 1 cut(s) 1950
Cfr13I GGNCC 1 cut(s) 2155
ClaI ATCGAT 1 cut(s) 93
CseI GACGC 1 cut(s) 181
Csp6I GTAC 7 cut(s) 241, 829, 1062, 1665, 1795, 2306, 2699
CspCI CAANNNNNGTGG 2 cut(s) 1077, 1112
CviQI GTAC 7 cut(s) 241, 829, 1062, 1665, 1795, 2306, 2699
DdeI CTNAG 4 cut(s) 1253, 1932, 2139, 2270
DpnI GATC 8 cut(s) 101, 296, 368, 716, 917, 1503, 1788, 2688
DpnII GATC 8 cut(s) 99, 294, 366, 714, 915, 1501, 1786, 2686
DraI TTTAAA 2 cut(s) 1732, 2497
DraIII CACNNNGTG 1 cut(s) 1173
DrdI GACNNNNNNGTC 1 cut(s) 909
DseDI GACNNNNNNGTC 1 cut(s) 909
Eam1104I CTCTTC 4 cut(s) 543, 1232, 1349, 2740
EarI CTCTTC 4 cut(s) 543, 1232, 1349, 2740
Eco130I CCWWGG 5 cut(s) 278, 308, 993, 1384, 2768
Eco24I GRGCYC 2 cut(s) 1597, 2776
Eco32I GATATC 1 cut(s) 1359
Eco47I GGWCC 1 cut(s) 2155
Eco57I CTGAAG 5 cut(s) 788, 1330, 1383, 1641, 2721
Eco88I CYCGRG 1 cut(s) 2093
EcoRI GAATTC 1 cut(s) 233
EcoRV GATATC 1 cut(s) 1359
EcoT14I CCWWGG 5 cut(s) 278, 308, 993, 1384, 2768
EcoT22I ATGCAT 3 cut(s) 1500, 2349, 2434
EcoT38I GRGCYC 2 cut(s) 1597, 2776
ErhI CCWWGG 5 cut(s) 278, 308, 993, 1384, 2768
FalI AAGNNNNNCTT 6 cut(s) 480, 512, 2002, 2034, 2214, 2246
FaqI GGGAC 2 cut(s) 433, 2020
FauI CCCGC 1 cut(s) 1503
FauNDI CATATG 1 cut(s) 2434
FbaI TGATCA 2 cut(s) 366, 714
FblI GTMKAC 1 cut(s) 1185
Fnu4HI GCNGC 2 cut(s) 1404, 2572
FokI GGATG 9 cut(s) 152, 546, 557, 595, 857, 869, 1193, 2228, 2326
FriOI GRGCYC 2 cut(s) 1597, 2776
Fsp4HI GCNGC 2 cut(s) 1404, 2572
FspBI CTAG 8 cut(s) 608, 794, 833, 1748, 1790, 2058, 2361, 2711
GlaI GCGC 1 cut(s) 1949
GluI GCNGC 2 cut(s) 1404, 2572
GsaI CCCAGC 1 cut(s) 2110
GsuI CTGGAG 1 cut(s) 2534
HaeIII GGCC 5 cut(s) 5, 206, 270, 1480, 2359
HapII CCGG 2 cut(s) 1067, 1597
HgaI GACGC 1 cut(s) 181
HhaI GCGC 1 cut(s) 1950
Hin6I GCGC 1 cut(s) 1948
HinP1I GCGC 1 cut(s) 1948
HincII GTYRAC 3 cut(s) 442, 1164, 1533
HindII GTYRAC 3 cut(s) 442, 1164, 1533
HindIII AAGCTT 4 cut(s) 1658, 2276, 2474, 2758
HinfI GANTC 8 cut(s) 313, 388, 467, 1278, 1327, 1571, 2165, 2732
HpaII CCGG 2 cut(s) 1067, 1597
HphI GGTGA 4 cut(s) 616, 2247, 2258, 2548
Hpy166II GTNNAC 7 cut(s) 442, 808, 1064, 1164, 1186, 1533, 1795
Hpy188I TCNGA 8 cut(s) 197, 393, 915, 933, 962, 1277, 1363, 2071
Hpy8I GTNNAC 7 cut(s) 442, 808, 1064, 1164, 1186, 1533, 1795
Hpy99I CGWCG 1 cut(s) 16
HpyCH4III ACNGT 5 cut(s) 673, 1080, 2250, 2524, 2664
HpyCH4IV ACGT 4 cut(s) 179, 328, 849, 2452
HpyF10VI GCNNNNNNNGC 5 cut(s) 510, 799, 1130, 1945, 2243
HpyF3I CTNAG 4 cut(s) 1253, 1932, 2139, 2270
HpySE526I ACGT 4 cut(s) 179, 328, 849, 2452
HspAI GCGC 1 cut(s) 1948
KpnI GGTACC 1 cut(s) 832
Ksp22I TGATCA 2 cut(s) 366, 714
Kzo9I GATC 8 cut(s) 99, 294, 366, 714, 915, 1501, 1786, 2686
LguI GCTCTTC 3 cut(s) 543, 1232, 1349
LmnI GCTCC 7 cut(s) 109, 291, 1015, 1069, 1513, 1592, 1843
Lsp1109I GCAGC 2 cut(s) 1415, 2558
LweI GCATC 6 cut(s) 130, 698, 1213, 1485, 2356, 2529
MaeI CTAG 8 cut(s) 608, 794, 833, 1748, 1790, 2058, 2361, 2711
MaeII ACGT 4 cut(s) 179, 328, 849, 2452
MaeIII GTNAC 5 cut(s) 1256, 1390, 1681, 2162, 2448
MalI GATC 8 cut(s) 101, 296, 368, 716, 917, 1503, 1788, 2688
MboI GATC 8 cut(s) 99, 294, 366, 714, 915, 1501, 1786, 2686
MfeI CAATTG 2 cut(s) 432, 1125
MflI RGATCY 1 cut(s) 915
MhlI GDGCHC 2 cut(s) 1597, 2776
MlyI GAGTC 5 cut(s) 322, 476, 1272, 2159, 2726
MmeI TCCRAC 2 cut(s) 893, 1300
Mph1103I ATGCAT 3 cut(s) 1500, 2349, 2434
MroXI GAANNNNTTC 5 cut(s) 128, 747, 1034, 2226, 2229
MslI CAYNNNNRTG 3 cut(s) 249, 362, 2342
MspI CCGG 2 cut(s) 1067, 1597
MspR9I CCNGG 1 cut(s) 1597
MunI CAATTG 2 cut(s) 432, 1125
Mva1269I GAATGC 1 cut(s) 1034
MwoI GCNNNNNNNGC 5 cut(s) 510, 799, 1130, 1945, 2243
NciI CCSGG 1 cut(s) 1597
NdeI CATATG 1 cut(s) 2434
NdeII GATC 8 cut(s) 99, 294, 366, 714, 915, 1501, 1786, 2686
NlaIV GGNNCC 5 cut(s) 287, 750, 830, 917, 1594
NmuCI GTSAC 3 cut(s) 1390, 1681, 2162
NsiI ATGCAT 3 cut(s) 1500, 2349, 2434
NspI RCATGY 2 cut(s) 1382, 1447
NspV TTCGAA 1 cut(s) 1330
PaeI GCATGC 1 cut(s) 1447
PaqCI CACCTGC 1 cut(s) 766
PciI ACATGT 1 cut(s) 1378
PciSI GCTCTTC 3 cut(s) 543, 1232, 1349
PctI GAATGC 1 cut(s) 1034
PdmI GAANNNNTTC 5 cut(s) 128, 747, 1034, 2226, 2229
PfeI GAWTC 3 cut(s) 388, 1327, 1571
PflMI CCANNNNNTGG 1 cut(s) 1173
PkrI GCNGC 2 cut(s) 1405, 2573
PleI GAGTC 5 cut(s) 321, 475, 1272, 2159, 2726
PpsI GAGTC 5 cut(s) 321, 475, 1272, 2159, 2726
PscI ACATGT 1 cut(s) 1378
PsiI TTATAA 2 cut(s) 1968, 1983
PspFI CCCAGC 1 cut(s) 2106
PspN4I GGNNCC 5 cut(s) 287, 750, 830, 917, 1594
PspPI GGNCC 1 cut(s) 2155
PsrI GAACNNNNNNTAC 2 cut(s) 960, 992
PstI CTGCAG 1 cut(s) 138
PstNI CAGNNNCTG 1 cut(s) 112
PsuI RGATCY 1 cut(s) 915
RsaI GTAC 7 cut(s) 242, 830, 1063, 1666, 1796, 2307, 2700
RsaNI GTAC 7 cut(s) 241, 829, 1062, 1665, 1795, 2306, 2699
RseI CAYNNNNRTG 3 cut(s) 249, 362, 2342
SapI GCTCTTC 3 cut(s) 543, 1232, 1349
SatI GCNGC 2 cut(s) 1404, 2572
Sau3AI GATC 8 cut(s) 99, 294, 366, 714, 915, 1501, 1786, 2686
Sau96I GGNCC 1 cut(s) 2155
SbfI CCTGCAGG 1 cut(s) 138
SchI GAGTC 5 cut(s) 322, 476, 1272, 2159, 2726
ScrFI CCNGG 1 cut(s) 1597
SdaI CCTGCAGG 1 cut(s) 138
SduI GDGCHC 2 cut(s) 1597, 2776
SfaNI GCATC 6 cut(s) 130, 698, 1213, 1485, 2356, 2529
SfcI CTRYAG 3 cut(s) 134, 781, 1407
SfuI TTCGAA 1 cut(s) 1330
SinI GGWCC 1 cut(s) 2155
SmiI ATTTAAAT 2 cut(s) 1732, 2497
SmiMI CAYNNNNRTG 3 cut(s) 249, 362, 2342
SmlI CTYRAG 3 cut(s) 1280, 1807, 2366
SmoI CTYRAG 3 cut(s) 1280, 1807, 2366
SphI GCATGC 1 cut(s) 1447
Sse8387I CCTGCAGG 1 cut(s) 138
SsiI CCGC 2 cut(s) 1397, 1510
SspMI CTAG 8 cut(s) 608, 794, 833, 1748, 1790, 2058, 2361, 2711
StyD4I CCNGG 1 cut(s) 1595
StyI CCWWGG 5 cut(s) 278, 308, 993, 1384, 2768
SwaI ATTTAAAT 2 cut(s) 1732, 2497
TaaI ACNGT 5 cut(s) 673, 1080, 2250, 2524, 2664
TaiI ACGT 4 cut(s) 182, 331, 852, 2455
TaqI TCGA 7 cut(s) 93, 1330, 2233, 2328, 2391, 2648, 2730
TatI WGTACW 2 cut(s) 1061, 1794
TfiI GAWTC 3 cut(s) 388, 1327, 1571
TscAI CASTG 5 cut(s) 82, 1624, 1690, 2253, 2529
TseFI GTSAC 3 cut(s) 1390, 1681, 2162
TseI GCWGC 2 cut(s) 1403, 2571
Tsp45I GTSAC 3 cut(s) 1390, 1681, 2162
TspRI CASTG 5 cut(s) 82, 1624, 1690, 2253, 2529
Van91I CCANNNNNTGG 1 cut(s) 1173
VpaK11BI GGWCC 1 cut(s) 2155
XapI RAATTY 5 cut(s) 233, 1819, 2225, 2482, 2498
XbaI TCTAGA 2 cut(s) 1747, 2057
XceI RCATGY 2 cut(s) 1382, 1447
XcmI CCANNNNNNNNNTGG 1 cut(s) 315
XmiI GTMKAC 1 cut(s) 1185
XmnI GAANNNNTTC 5 cut(s) 128, 747, 1034, 2226, 2229
XspI CTAG 8 cut(s) 608, 794, 833, 1748, 1790, 2058, 2361, 2711
Zsp2I ATGCAT 3 cut(s) 1500, 2349, 2434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.