Prupe.6G199400_v2.0.a1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000110
Physical Location & Seq
Forward (+)
20610911 .. 20614264
3354 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G199400.2

Sequence Viewer

Length: 2739 bp
ATGGCGTCAGTTTCAGCAGACCTCCTCATTTGCAAATTTGTAGCTATTCTCGAGAGTGAAGCAGCCTCCATAGCGGGTGTTGGTGATCATGTTGATGAGATTAAGCGGGAGCTGGAATTCATGAAAGCCTTCTTAGCGAATGCTGACGAGGGCAACAAAGCACACACACAAGTAGAGCAAGTGTGGATCGTAAGTGTTCGAGACTTGGTCAATGATGTTGAAAACATCATTGATGAATTCATGTATCACGTGTACGAGCAGCAAATTGGCTGTCGATTTGCCAGGTGGATCCACAAAACCATTCACTTTCCAAAGCACCTTTGGTCTAAGCGTAAAATAGCCAACAAGTTACAGAAAATCGCGATGGCAATTAGAGCTATTACAGAGAGAACTGTAGAAGGAAAAAGTACTTCTTCTGAGGATATTCGCAGATGGGTGCAGATTCAAGCGGTGTCTTCTCTTTATCATAAGGAGGATGAACTCGTCGGGATTGAAGGTGATAAGAACTTGTTAATAGGATGGCTGACGAACGAAGAGCAACGCCAAACTGTTGTGTCTGTGGTCGGGATGGGAGGATCGGGGAAGACAACTCTAGTTGCAAGGACTTTCAAAGATGAAATTGTAAAGAGACATTTCGAATGCTATGCGTGGATTACTATTTCCCAGTCTTATGTGATTGAAGACTTACTTAGAAGATTGATCAAAGAATTCCACAAAGCAAAGAAGGAAGAGTTCCCTGCAGACATGAATGCCATGAGTTATAACGAATTGCTGGAGATTTTGGTGAACTACTTGGAGACTAAAAGGTACCTAGTTGTATTGGATGATGTGTGGGATGTCCACCTTTGGGAGAAAATAAGGTTTTCATTTCCAGATAAACAACTTGGAAGTCGAGTCATGCTTACAACTCGAAGAGAAGACATAGCATCCTCTTCTTTTGGAGTTGAAAGCCATGTTCACAAGATTCAACCGCTGGAAAAAGGTGATGCGTGGGAGCTCTTCAGCATGAAAGCATTCTCAAGTTATCCAAACAAATCTTGTTCACCTGAACTTCTGCCTTTAGCTCGGGAACTTGTGGAGAAGTGTGAAGGTCTACCTCTAGCGATCGTAGCCTTGAGTGGTCTCATGTCTTCTAAGAAGTCACTTACAGAGTGGAGCACAGTCTACAACAGCTTAAATTGGCATTTAACAAACAGTCCTTTGCTAGAACCTATGAAGATGCGCATCTTGTTGTTTAGTTTTAATGATTTGCCATACCGATTGAAGCAATGCTTTCTATATTGTTCACTTTTCCCTGAAGATCATGTGATCTTAAATCTTAGGCTCATCACATTGTGGATCGCTGAAGGATTTGTTGAACATGTCGAAGGGCTCACGCCAGAAGAAGTTGCAAATAGCTATCTTATGGAACTCTTTTTTCGTAACATGCTACAGCAAAGGTTTCGAGGACCCCTCCCAGCATGTAAGATGCATGATCTTTTGCGGGAGATTGCCTTGTCTATAGCAAAAGAAGAAAAGTTTTGCACTGTACATGATGGGAGTGAAACAGTAGAAGAAACTGGAGCACTCCGTTTGTCAATTCAAACAACTAATGGAGAAATTGGATCATGCACAGGTATATCACGGCTTTGTTCGTTTCTCGTCTTTGCCACTGACTTATCCTCATTCTCTTTACCAAATAAATTTACTTCTGACCTTAAATTGTTGAAGGTTCTAGATTTGGAGGATGTCCCAATTGATAATCTGCCAGACAATCTAACATCCTTATTTAATTTAAAATACTTAAATTTGAGTCGAACTCCAATTACGGAGCTTCCGGAATCCATTAGGCAGCTCCACAACCTTCAGACCTTGAACATCACGGGGACAAAAATAGAGGCACTTCCAAGAGGGATTTCCAAGTTGCTAAAGTTACGCCATTTACTTATGGGTAGGTTCATTTCCAGAAAGATAATTGGGGTAAGAATACCATCAAGTATTAGTAAGATGAAGAAATTGCAAACTTTGGAAAATATTGAATCAAAAGGAAACATTATTAGACTTATTGGAAGTATGACCCAGCTTAAGTTCCTCGGCATTACAAATGTGAAAGAAAGAGACGAGGAGGACCTATGTGCCTCAATTCAAGAGATGAAGGTCCTTTCCCGCTTGCTTTTATTTGTAGCTGATGGAGAGGAATTTCTTCGAGTTGACGCATTGTCTTCACCGCCTCCCTACCTTGATAGACTTAGATTGGTCGGCAAACTGGAAAAGGTACCACAACGGTTTTGTTCACTCCACTCTCTCGCATATTTGAATCTGCGTGGGTCTAGACTTGAAGAAGATTTTCTACCTCATATTGAAGCATTGCCCTCTCTACGTTCTCTTTGGCTTGATAATACCTCTGTTAAGAAAGAGTTATGTTTCAACAGAGGCTTTGTGAAGCTTTGGTATTTGCAGTTTCAGAATTTCGCATTATTAAATAAGATAACTATAGAAAAAGGGACGATGCCAAATCTTGAGTTCTTAGACATTCGTAGTTGCATGACGTTAGAAACATTGCCACAGGGTATTGAGCACCTTATTAAACTACGAGGATACAGATTTGATAATGTTTCAGAGAAGTTTAGAGAGTCCATAAAAGGAGGAGGTGTGGATCATCCAAGGATGCTACTCGTCGACGAGAAATGTAAGAAACATATAAACAAGTCCACCCTGATTGAAGAATTTACCCATGAGTACTCTTTTTACTCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

913

Amino Acids

104.79

Weight (kDa)

6.82

Isoelectric Point (pI)

52.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 762
Acc16I TGCGCA 1 cut(s) 1223
Acc65I GGTACC 2 cut(s) 807, 2254
AccB1I GGYRCC 2 cut(s) 807, 2254
AccI GTMKAC 3 cut(s) 1093, 1164, 2658
AccII CGCG 1 cut(s) 362
AccIII TCCGGA 1 cut(s) 1816
AciI CCGC 7 cut(s) 74, 106, 449, 971, 1483, 2146, 2207
AclWI GGATC 7 cut(s) 194, 283, 296, 583, 1346, 1612, 2643
AcsI RAATTY 9 cut(s) 35, 116, 236, 707, 1682, 1786, 2177, 2446, 2705
AcuI CTGAAG 4 cut(s) 985, 1317, 1365, 1829
AcvI CACGTG 1 cut(s) 250
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 1335
AfaI GTAC 6 cut(s) 254, 409, 809, 1530, 2256, 2720
AfiI CCNNNNNNNGG 1 cut(s) 847
AflII CTTAAG 1 cut(s) 2063
AflIII ACRYGT 2 cut(s) 249, 1360
AhdI GACNNNNNGTC 1 cut(s) 2197
AjnI CCWGG 1 cut(s) 281
AjuI GAANNNNNNNTTGG 2 cut(s) 1879, 1911
Alw21I GWGCWC 4 cut(s) 999, 1160, 1567, 2559
Alw26I GTCTC 5 cut(s) 195, 622, 791, 1127, 2091
AlwI GGATC 7 cut(s) 194, 283, 296, 583, 1346, 1612, 2643
Ama87I CYCGRG 2 cut(s) 50, 1065
Aor13HI TCCGGA 1 cut(s) 1816
ApeKI GCWGC 3 cut(s) 62, 259, 1831
ApoI RAATTY 9 cut(s) 35, 116, 236, 707, 1682, 1786, 2177, 2446, 2705
ArsI GACNNNNNNTTYG 2 cut(s) 256, 288
Asp700I GAANNNNTTC 4 cut(s) 128, 1013, 2181, 2325
Asp718I GGTACC 2 cut(s) 807, 2254
AspLEI GCGC 1 cut(s) 1224
AspS9I GGNCC 3 cut(s) 1448, 2107, 2137
AsuHPI GGTGA 6 cut(s) 95, 509, 796, 995, 1035, 2196
AsuII TTCGAA 1 cut(s) 636
AvaI CYCGRG 2 cut(s) 50, 1065
AvaII GGWCC 3 cut(s) 1448, 2107, 2137
BamHI GGATCC 1 cut(s) 288
BanI GGYRCC 2 cut(s) 807, 2254
BanII GRGCYC 2 cut(s) 999, 1374
BarI GAAGNNNNNNTAC 2 cut(s) 2313, 2345
BbrPI CACGTG 1 cut(s) 250
BbsI GAAGAC 6 cut(s) 447, 590, 687, 924, 1122, 2193
Bbv12I GWGCWC 4 cut(s) 999, 1160, 1567, 2559
BbvI GCAGC 3 cut(s) 74, 271, 1843
BccI CCATC 7 cut(s) 358, 426, 513, 562, 1529, 1978, 2162
BceAI ACGGC 1 cut(s) 1640
BcgI CGANNNNNNTGC 4 cut(s) 626, 660, 1424, 1458
BciT130I CCWGG 1 cut(s) 283
BciVI GTATCC 1 cut(s) 2570
BclI TGATCA 2 cut(s) 85, 699
BcoDI GTCTC 5 cut(s) 195, 622, 791, 1127, 2091
BfaI CTAG 6 cut(s) 593, 812, 1100, 1205, 1715, 2310
BfmI CTRYAG 5 cut(s) 393, 738, 1430, 1500, 2472
BfrI CTTAAG 1 cut(s) 2063
BfuI GTATCC 1 cut(s) 2570
BisI GCNGC 3 cut(s) 63, 260, 1832
BlsI GCNGC 3 cut(s) 64, 261, 1833
BmcAI AGTACT 2 cut(s) 409, 2720
Bme1390I CCNGG 1 cut(s) 283
Bme18I GGWCC 3 cut(s) 1448, 2107, 2137
BmeRI GACNNNNNGTC 1 cut(s) 2197
BmeT110I CYCGRG 2 cut(s) 50, 1065
BmgT120I GGNCC 3 cut(s) 1448, 2107, 2137
BmiI GGNNCC 4 cut(s) 290, 809, 1450, 2256
BmrFI CCNGG 1 cut(s) 283
BmrI ACTGGG 1 cut(s) 658
BmsI GCATC 7 cut(s) 935, 976, 1209, 1233, 1458, 2478, 2637
BmuI ACTGGG 1 cut(s) 658
BpiI GAAGAC 6 cut(s) 447, 590, 687, 924, 1122, 2193
BplI GAGNNNNNCTC 4 cut(s) 1437, 1469, 1783, 1815
BpmI CTGGAG 2 cut(s) 794, 1581
Bpu14I TTCGAA 1 cut(s) 636
BpuEI CTTGAG 3 cut(s) 1003, 1135, 2519
BsaAI YACGTR 1 cut(s) 250
BsaBI GATNNNNATC 1 cut(s) 1223
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1127
BsaJI CCNNGG 2 cut(s) 2071, 2642
BsaWI WCCGGW 1 cut(s) 1816
BsaXI ACNNNNNCTCC 2 cut(s) 2616, 2646
Bsc4I CCNNNNNNNGG 1 cut(s) 847
Bse1I ACTGG 3 cut(s) 664, 1564, 2250
Bse3DI GCAATG 3 cut(s) 1274, 2345, 2537
Bse8I GATNNNNATC 1 cut(s) 1223
BseAI TCCGGA 1 cut(s) 1816
BseBI CCWGG 1 cut(s) 283
BseDI CCNNGG 2 cut(s) 2071, 2642
BseJI GATNNNNATC 1 cut(s) 1223
BseLI CCNNNNNNNGG 1 cut(s) 847
BseMI GCAATG 3 cut(s) 1274, 2345, 2537
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 3 cut(s) 664, 1564, 2250
BseRI GAGGAG 3 cut(s) 14, 2117, 2640
BseXI GCAGC 3 cut(s) 74, 271, 1843
BseYI CCCAGC 2 cut(s) 1456, 2058
BsgI GTGCAG 1 cut(s) 458
Bsh1236I CGCG 1 cut(s) 362
Bsh1285I CGRYCG 1 cut(s) 1107
BshNI GGYRCC 2 cut(s) 807, 2254
BsiEI CGRYCG 1 cut(s) 1107
BsiHKAI GWGCWC 4 cut(s) 999, 1160, 1567, 2559
BsiHKCI CYCGRG 2 cut(s) 50, 1065
BsiSI CCGG 1 cut(s) 1817
BslFI GGGAC 3 cut(s) 1715, 1879, 2497
BslI CCNNNNNNNGG 1 cut(s) 847
BsmAI GTCTC 5 cut(s) 195, 622, 791, 1127, 2091
BsmBI CGTCTC 1 cut(s) 2091
BsmFI GGGAC 3 cut(s) 1715, 1879, 2497
BsmI GAATGC 4 cut(s) 145, 644, 754, 1013
Bso31I GGTCTC 1 cut(s) 1127
BsoBI CYCGRG 2 cut(s) 50, 1065
Bsp119I TTCGAA 1 cut(s) 636
Bsp1286I GDGCHC 5 cut(s) 999, 1160, 1374, 1567, 2559
Bsp13I TCCGGA 1 cut(s) 1816
Bsp1407I TGTACA 1 cut(s) 1528
Bsp68I TCGCGA 1 cut(s) 362
BspACI CCGC 7 cut(s) 74, 106, 449, 971, 1483, 2146, 2207
BspCNI CTCAG 1 cut(s) 409
BspEI TCCGGA 1 cut(s) 1816
BspFNI CGCG 1 cut(s) 362
BspHI TCATGA 1 cut(s) 120
BspLI GGNNCC 4 cut(s) 290, 809, 1450, 2256
BspMAI CTGCAG 1 cut(s) 742
BspPI GGATC 7 cut(s) 194, 283, 296, 583, 1346, 1612, 2643
BspQI GCTCTTC 2 cut(s) 528, 1004
BspT104I TTCGAA 1 cut(s) 636
BspT107I GGYRCC 2 cut(s) 807, 2254
BspTI CTTAAG 1 cut(s) 2063
BspTNI GGTCTC 1 cut(s) 1127
BsrDI GCAATG 3 cut(s) 1274, 2345, 2537
BsrGI TGTACA 1 cut(s) 1528
BsrI ACTGG 3 cut(s) 664, 1564, 2250
BssECI CCNNGG 2 cut(s) 2071, 2642
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 2642
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 7 cut(s) 394, 550, 1162, 1196, 1528, 1549, 2265
Bst6I CTCTTC 5 cut(s) 528, 723, 907, 937, 1004
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 2063
BstAUI TGTACA 1 cut(s) 1528
BstBAI YACGTR 1 cut(s) 250
BstBI TTCGAA 1 cut(s) 636
BstC8I GCNNGC 1 cut(s) 2150
BstDEI CTNAG 8 cut(s) 133, 327, 417, 689, 1134, 1319, 2228, 2506
BstFNI CGCG 1 cut(s) 362
BstHHI GCGC 1 cut(s) 1224
BstMAI GTCTC 5 cut(s) 195, 622, 791, 1127, 2091
BstMCI CGRYCG 1 cut(s) 1107
BstMWI GCNNNNNNNGC 4 cut(s) 71, 134, 374, 1109
BstNI CCWGG 1 cut(s) 283
BstNSI RCATGY 3 cut(s) 1364, 1429, 1464
BstSCI CCNGG 1 cut(s) 281
BstSFI CTRYAG 5 cut(s) 393, 738, 1430, 1500, 2472
BstUI CGCG 1 cut(s) 362
BstV1I GCAGC 3 cut(s) 74, 271, 1843
BstV2I GAAGAC 6 cut(s) 447, 590, 687, 924, 1122, 2193
BstX2I RGATCY 1 cut(s) 288
BstYI RGATCY 1 cut(s) 288
BsuI GTATCC 1 cut(s) 2570
BtgZI GCGATG 1 cut(s) 377
BtsIMutI CAGTG 2 cut(s) 1524, 1650
BtuMI TCGCGA 1 cut(s) 362
Cac8I GCNNGC 1 cut(s) 2150
CciI TCATGA 1 cut(s) 120
CfoI GCGC 1 cut(s) 1224
Cfr13I GGNCC 3 cut(s) 1448, 2107, 2137
CseI GACGC 1 cut(s) 2201
Csp6I GTAC 6 cut(s) 253, 408, 808, 1529, 2255, 2719
CspCI CAANNNNNGTGG 2 cut(s) 2680, 2715
CviQI GTAC 6 cut(s) 253, 408, 808, 1529, 2255, 2719
DdeI CTNAG 8 cut(s) 133, 327, 417, 689, 1134, 1319, 2228, 2506
DraI TTTAAA 1 cut(s) 1776
DraIII CACNNNGTG 1 cut(s) 1335
DriI GACNNNNNGTC 1 cut(s) 2197
Eam1104I CTCTTC 5 cut(s) 528, 723, 907, 937, 1004
Eam1105I GACNNNNNGTC 1 cut(s) 2197
EarI CTCTTC 5 cut(s) 528, 723, 907, 937, 1004
Ecl136II GAGCTC 1 cut(s) 997
Eco130I CCWWGG 1 cut(s) 2642
Eco24I GRGCYC 2 cut(s) 999, 1374
Eco31I GGTCTC 1 cut(s) 1127
Eco47I GGWCC 3 cut(s) 1448, 2107, 2137
Eco53kI GAGCTC 1 cut(s) 997
Eco57I CTGAAG 4 cut(s) 985, 1317, 1365, 1829
Eco72I CACGTG 1 cut(s) 250
Eco88I CYCGRG 2 cut(s) 50, 1065
EcoICRI GAGCTC 1 cut(s) 997
EcoO109I RGGNCCY 3 cut(s) 1448, 2107, 2137
EcoRI GAATTC 3 cut(s) 116, 236, 707
EcoRII CCWGG 1 cut(s) 281
EcoT14I CCWWGG 1 cut(s) 2642
EcoT22I ATGCAT 1 cut(s) 1473
EcoT38I GRGCYC 2 cut(s) 999, 1374
ErhI CCWWGG 1 cut(s) 2642
Esp3I CGTCTC 1 cut(s) 2091
FalI AAGNNNNNCTT 6 cut(s) 397, 429, 672, 704, 1256, 1288
FaqI GGGAC 3 cut(s) 1715, 1879, 2497
FauI CCCGC 4 cut(s) 67, 99, 1476, 2153
FbaI TGATCA 2 cut(s) 85, 699
FblI GTMKAC 3 cut(s) 1093, 1164, 2658
Fnu4HI GCNGC 3 cut(s) 63, 260, 1832
FriOI GRGCYC 2 cut(s) 999, 1374
Fsp4HI GCNGC 3 cut(s) 63, 260, 1832
FspAI RTGCGCAY 1 cut(s) 1223
FspBI CTAG 6 cut(s) 593, 812, 1100, 1205, 1715, 2310
FspI TGCGCA 1 cut(s) 1223
GlaI GCGC 1 cut(s) 1223
GluI GCNGC 3 cut(s) 63, 260, 1832
GsaI CCCAGC 2 cut(s) 1460, 2062
GsuI CTGGAG 2 cut(s) 794, 1581
HapII CCGG 1 cut(s) 1817
HgaI GACGC 1 cut(s) 2201
HhaI GCGC 1 cut(s) 1224
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 1222
HinP1I GCGC 1 cut(s) 1222
HincII GTYRAC 2 cut(s) 2191, 2659
HindII GTYRAC 2 cut(s) 2191, 2659
HindIII AAGCTT 1 cut(s) 2423
HinfI GANTC 8 cut(s) 442, 894, 964, 1792, 1820, 2018, 2296, 2612
HpaII CCGG 1 cut(s) 1817
HphI GGTGA 6 cut(s) 95, 509, 796, 995, 1035, 2196
Hpy188I TCNGA 5 cut(s) 418, 1693, 1848, 2445, 2599
Hpy99I CGWCG 3 cut(s) 488, 2660, 2663
HpyCH4III ACNGT 7 cut(s) 394, 550, 1162, 1196, 1528, 1549, 2265
HpyCH4IV ACGT 3 cut(s) 249, 2359, 2528
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 134, 374, 1109
HpyF3I CTNAG 8 cut(s) 133, 327, 417, 689, 1134, 1319, 2228, 2506
HpySE526I ACGT 3 cut(s) 249, 2359, 2528
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 1222
Kpn2I TCCGGA 1 cut(s) 1816
KpnI GGTACC 2 cut(s) 811, 2258
Ksp22I TGATCA 2 cut(s) 85, 699
LguI GCTCTTC 2 cut(s) 528, 1004
LmnI GCTCC 6 cut(s) 109, 994, 1155, 1562, 1810, 1839
Lsp1109I GCAGC 3 cut(s) 74, 271, 1843
LweI GCATC 7 cut(s) 935, 976, 1209, 1233, 1458, 2478, 2637
MaeI CTAG 6 cut(s) 593, 812, 1100, 1205, 1715, 2310
MaeII ACGT 3 cut(s) 249, 2359, 2528
MaeIII GTNAC 4 cut(s) 348, 1140, 1421, 1911
MfeI CAATTG 1 cut(s) 1734
MflI RGATCY 1 cut(s) 288
MhlI GDGCHC 5 cut(s) 999, 1160, 1374, 1567, 2559
MlyI GAGTC 3 cut(s) 903, 1801, 2621
Mph1103I ATGCAT 1 cut(s) 1473
MroI TCCGGA 1 cut(s) 1816
MroXI GAANNNNTTC 4 cut(s) 128, 1013, 2181, 2325
MslI CAYNNNNRTG 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 973
MspCI CTTAAG 1 cut(s) 2063
MspI CCGG 1 cut(s) 1817
MspR9I CCNGG 1 cut(s) 283
MunI CAATTG 1 cut(s) 1734
Mva1269I GAATGC 4 cut(s) 145, 644, 754, 1013
MvaI CCWGG 1 cut(s) 283
MvnI CGCG 1 cut(s) 362
MwoI GCNNNNNNNGC 4 cut(s) 71, 134, 374, 1109
NlaIV GGNNCC 4 cut(s) 290, 809, 1450, 2256
NmeAIII GCCGAG 1 cut(s) 2052
NmuCI GTSAC 1 cut(s) 1140
NruI TCGCGA 1 cut(s) 362
NsbI TGCGCA 1 cut(s) 1223
NsiI ATGCAT 1 cut(s) 1473
NspI RCATGY 3 cut(s) 1364, 1429, 1464
NspV TTCGAA 1 cut(s) 636
PaeR7I CTCGAG 1 cut(s) 50
PagI TCATGA 1 cut(s) 120
PciI ACATGT 1 cut(s) 1360
PciSI GCTCTTC 2 cut(s) 528, 1004
PctI GAATGC 4 cut(s) 145, 644, 754, 1013
PdmI GAANNNNTTC 4 cut(s) 128, 1013, 2181, 2325
PfeI GAWTC 5 cut(s) 442, 964, 1820, 2018, 2296
PflFI GACNNNGTC 1 cut(s) 206
PkrI GCNGC 3 cut(s) 64, 261, 1833
Ple19I CGATCG 1 cut(s) 1107
PleI GAGTC 3 cut(s) 902, 1800, 2620
PmaCI CACGTG 1 cut(s) 250
PmlI CACGTG 1 cut(s) 250
PpsI GAGTC 3 cut(s) 902, 1800, 2620
Ppu21I YACGTR 1 cut(s) 250
PpuMI RGGWCCY 3 cut(s) 1448, 2107, 2137
PscI ACATGT 1 cut(s) 1360
PsiI TTATAA 1 cut(s) 762
Psp124BI GAGCTC 1 cut(s) 999
Psp5II RGGWCCY 3 cut(s) 1448, 2107, 2137
Psp6I CCWGG 1 cut(s) 281
PspCI CACGTG 1 cut(s) 250
PspFI CCCAGC 2 cut(s) 1456, 2058
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 4 cut(s) 290, 809, 1450, 2256
PspPI GGNCC 3 cut(s) 1448, 2107, 2137
PspPPI RGGWCCY 3 cut(s) 1448, 2107, 2137
PsrI GAACNNNNNNTAC 2 cut(s) 1789, 1821
PstI CTGCAG 1 cut(s) 742
PsuI RGATCY 1 cut(s) 288
PsyI GACNNNGTC 1 cut(s) 206
PvuI CGATCG 1 cut(s) 1107
RruI TCGCGA 1 cut(s) 362
RsaI GTAC 6 cut(s) 254, 409, 809, 1530, 2256, 2720
RsaNI GTAC 6 cut(s) 253, 408, 808, 1529, 2255, 2719
RseI CAYNNNNRTG 1 cut(s) 93
SacI GAGCTC 1 cut(s) 999
SalI GTCGAC 1 cut(s) 2657
SapI GCTCTTC 2 cut(s) 528, 1004
SatI GCNGC 3 cut(s) 63, 260, 1832
Sau96I GGNCC 3 cut(s) 1448, 2107, 2137
ScaI AGTACT 2 cut(s) 409, 2720
SchI GAGTC 3 cut(s) 903, 1801, 2621
ScrFI CCNGG 1 cut(s) 283
SduI GDGCHC 5 cut(s) 999, 1160, 1374, 1567, 2559
SfaNI GCATC 7 cut(s) 935, 976, 1209, 1233, 1458, 2478, 2637
SfcI CTRYAG 5 cut(s) 393, 738, 1430, 1500, 2472
Sfr274I CTCGAG 1 cut(s) 50
SfuI TTCGAA 1 cut(s) 636
SgrDI CGTCGACG 1 cut(s) 2657
SinI GGWCC 3 cut(s) 1448, 2107, 2137
SlaI CTCGAG 1 cut(s) 50
SmiMI CAYNNNNRTG 1 cut(s) 93
SmlI CTYRAG 5 cut(s) 50, 1018, 1114, 2063, 2498
SmoI CTYRAG 5 cut(s) 50, 1018, 1114, 2063, 2498
SsiI CCGC 7 cut(s) 74, 106, 449, 971, 1483, 2146, 2207
SspI AATATT 1 cut(s) 2014
SspMI CTAG 6 cut(s) 593, 812, 1100, 1205, 1715, 2310
SstI GAGCTC 1 cut(s) 999
StyD4I CCNGG 1 cut(s) 281
StyI CCWWGG 1 cut(s) 2642
TaaI ACNGT 7 cut(s) 394, 550, 1162, 1196, 1528, 1549, 2265
TaiI ACGT 3 cut(s) 252, 2362, 2531
TatI WGTACW 3 cut(s) 407, 1528, 2718
TfiI GAWTC 5 cut(s) 442, 964, 1820, 2018, 2296
TscAI CASTG 2 cut(s) 1531, 1657
TseFI GTSAC 1 cut(s) 1140
TseI GCWGC 3 cut(s) 62, 259, 1831
Tsp45I GTSAC 1 cut(s) 1140
TspGWI ACGGA 2 cut(s) 1559, 1823
TspRI CASTG 2 cut(s) 1531, 1657
Tth111I GACNNNGTC 1 cut(s) 206
Vha464I CTTAAG 1 cut(s) 2063
VpaK11BI GGWCC 3 cut(s) 1448, 2107, 2137
XapI RAATTY 9 cut(s) 35, 116, 236, 707, 1682, 1786, 2177, 2446, 2705
XbaI TCTAGA 2 cut(s) 1714, 2309
XceI RCATGY 3 cut(s) 1364, 1429, 1464
XcmI CCANNNNNNNNNTGG 1 cut(s) 318
XhoI CTCGAG 1 cut(s) 50
XmiI GTMKAC 3 cut(s) 1093, 1164, 2658
XmnI GAANNNNTTC 4 cut(s) 128, 1013, 2181, 2325
XspI CTAG 6 cut(s) 593, 812, 1100, 1205, 1715, 2310
ZrmI AGTACT 2 cut(s) 409, 2720
Zsp2I ATGCAT 1 cut(s) 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.