Rw5G032330

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000110
Physical Location & Seq
Reverse (-)
54577411 .. 54590068
12658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G032330.1

Sequence Viewer

Length: 2781 bp
ATGGCCTTGTCTGTAACGGACCTCTTAATTGGAAAACTTGCGACCATTCTTGAGTACGAAGGAACAACCATAGCAGGTGTTCGTCATGAAGTTGATAAGATTAAGGAGGAGTTTCTAAGCATGAAAGCTTTCCTACTGGATGCGGAGGCCAACAAACCAAAAACTGAAGGACAGAAATTGTGGGTTGCAAGAATCAGAGACTTAGCCTATGATGTTGAAGATATCATTGACGAATTCATGTATCACATGTATGAGAAGCAAAGTGGCGGTCGACTCTCAAGGAGGCTACACAAAACCATTCGCGCTCCATGTAATATTTGGTTCAGGCATAAAATTGCAAAGAAGTTGCGGAAAATTATTGACATGATCAAAGCCATTCCAGAGAGGAATAAAAGATACGGTGTTGGACTTGTAGGAGGAACAACTACATCCGAAGATATTCTGAAATTCGTACAGAACCAAGCGGAGTCTTCGTTTTTCATTATGGAAGGCGAACTGGTTGGGATTGAAGGCAAGAAGCAAATACTAATGGGATGGCTAATGGATGACGAGCAACACCAAACTGTTATATCTGTGGTAGGAATGGGAGGTGCTGGAAAGACAACTCTAGTTGCCAAGACCTTCACCAATGAACTGGTAAAGAGACATTTTGATTGTTATGCATGGATTATTGTTTCCCAAACTTATGATGTGGTACACTTGTTTCGAAGCATGATCAGGGAACTCTACAAATCAAGGAAGGAAGATTTCCCTGCAAATTTGAATGCCATGAGCCGCATAGAATTGCTAGAATTACTAGTTAACTACTTGGAGTCCAAAAAATACCTAGTGGTTCTAGATGACGTGTGGGATATAAAAATATGGAGAGAAATAAAGGTATCACTTCAAGATAGACAACTTGGAAGTCGAGTCATACTTACAACTCGAAATGAAGAAGTAGCATCCAACTCTTTTGGAGTTGAAAGCTATGTTTACCGTATTCAGCCCCTGCAACAGAATGAGGCTTGGGAACTCTTCAGCAGGAAAGCATTCTCAACTAAGCCACACAAAACTTGTCCAACTGAGCTGAAGTCATTAGCTTGCCAACTTGTGGAGAAGTGTGAAGGCCTTCCTCTCGGCATTGTGGCTTTAGGTGGTCTTATGTCTTCTAAGAAGTCGCTGGATCAATGGCAACAAGTCTGCAACAACTTGAATTGGTATCTACATAACAATTCATCCCTAGACCCTCTGAGAAACATTTTGTTGCTGAGTTTCAATGATTTGCCATCTCAACTGAAGCATTGCTTCCTCTATTGTTCCCTTTTTCCAGAAGATTATCTATTTAAAAGAAAAAGGATGACTAGATTGTGGATAGCGGAAGGATTTGTTGAAAATGTGAAAGGGGTCACGCCCGAAGAAGTTGCAGATGGCTATCTTCAGGAACTCTGCTTCCTTAGCATGTTACTGGTTGCAGATGACTTTGTTTCCGCAGCATCTTCGGGAAGGCTGAAAAAATTTAAGATGCATGATGTGTTGCGAGAGCTTGCTTTGTTGACAGCCAAAAATGAAAAGTTTGGTTATGTATATGACGGGAGAGAAGTAATGGAGGAGATCTCAACCCGTCGCTTGTCAATTCACAGAATGGAAGGAGAAATTAAATCATGGCGGGGTATGTCAAAGATTTGTTCTCTTCTTGTATTTGCCACTGTCATGTCCTCATTGTATTTCTTAAATACACTGGTTTCTCGATTCAAATTGTTGAGGACTCTAGACTTGGAGGATGTCCAAATTGATAATCTGCCGGGTGCAGTTGTTTACTTGTTCAACTTGAGATATTTAAATTTGAAGGGCACTTTAATTAAGGAACTTCCAAAGTCCATAAGGTGTCTCCGCAACCTTCAATTTCTGAACATCAGGGATACTAAGATAGAGTCTGTCAGGACCCACCCCGGAATTTCCCTCCTAACTCCTAGGTTGCTGAGCCTGCGCAATCTAATTATGTATCGCTACACTGGGAACCTGTACTTCAAAGTTGTGAGTGGGACAAAAGCGCCATCAAATATTTGTAAGTTGCAGAAATTGCAAAGTTTATTACTAATTGAATCAGAAGGGGACACTTGTAGACTTATCGGCAACATGACTCAATTTAAGAGCATCGGCATATCGAATGTGAAAGAAAGAGATGAGATTGACCTTTGGCTCTCAATTAAGAAGTTCACTTTGCTTCAACGTCTAGCTTTAACGGCAAGTAATGAAGAGGAAATCCTTCCGGTGAAAGCACAATGTCCTCCTCTTCCACACCTTCAAAAGCTTATTTTGGTTGGCAGACTGCAAAACGTAATATCTTGGTTTTCTTCACTGCATAGCCTCACAACTCTGTTTCTGCATTGGTCAAGACTTGATGAGGATTTACTACCTCAAATTGAAGCTCTGCTCAATCTGACAAGGCTTTATATTTGTAATGCATATGTTGGGCAAGAGTTGTGTTTCCGTAGAGGCTTTGTAAAGCTGATGAGGTTGGAGTTGTTGAATTTTGCCTCGGTGAAAAAGATAACTATAGAAAATGGGGTGATGCCAAATCTTCGGTACTTATTCGTTGACAGCTGCATGGAGTTGAAGACAGTGCCATTGGGCCTTGAATATCTTTCTACTCTACAATATTTGGGCCTAAAAGATGTTCCCATGGAAGTTATAAGGCCCATTCAGAAAGGAGGTGTGGATCGCTCCAAGGTACAACACATTTCTGAGATCATACATGTTTCCAAACAGTCATCCAAGTGGGTCCACGAAAACTTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

926

Amino Acids

106.53

Weight (kDa)

9.05

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 93 6.5e-23 Rx N-terminal domain
NB-ARC PF00931 172 - 346 6.3e-47 NB-ARC domain
WHD_DRP PF23559 434 - 509 2.3e-17 Disease resistance protein Winged helix domain
LRR_14 PF23598 556 - 880 1.2e-27 Leucine-rich repeat region
LRR_8 PF13855 580 - 636 2.6e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2672
AarI CACCTGC 1 cut(s) 65
Acc16I TGCGCA 1 cut(s) 1967
Acc36I ACCTGC 1 cut(s) 65
AccB7I CCANNNNNTGG 1 cut(s) 1090
AccI GTMKAC 2 cut(s) 271, 2101
AccII CGCG 1 cut(s) 303
AciI CCGC 9 cut(s) 143, 267, 349, 464, 775, 1355, 1467, 1645, 1870
AclWI GGATC 2 cut(s) 1170, 2706
AcsI RAATTY 7 cut(s) 233, 446, 757, 1493, 1819, 1932, 2509
AcuI CTGAAG 5 cut(s) 186, 1000, 1088, 1295, 1400
AfaI GTAC 6 cut(s) 56, 453, 696, 2003, 2567, 2712
AfiI CCNNNNNNNGG 1 cut(s) 1090
AflIII ACRYGT 3 cut(s) 246, 843, 2734
AhlI ACTAGT 1 cut(s) 796
AjiI CACGTC 1 cut(s) 844
AloI GAACNNNNNNTCC 2 cut(s) 1413, 1445
Alw26I GTCTC 3 cut(s) 192, 637, 1871
AlwI GGATC 2 cut(s) 1170, 2706
AlwNI CAGNNNCTG 1 cut(s) 988
AoxI GGCC 7 cut(s) 3, 147, 1105, 2611, 2644, 2675, 2775
ApeKI GCWGC 2 cut(s) 1469, 2583
ApoI RAATTY 7 cut(s) 233, 446, 757, 1493, 1819, 1932, 2509
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2244
AspA2I CCTAGG 1 cut(s) 1949
AspLEI GCGC 3 cut(s) 305, 1968, 2032
AspS9I GGNCC 6 cut(s) 19, 1920, 2611, 2644, 2676, 2761
AsuC2I CCSGG 2 cut(s) 1782, 1929
AsuHPI GGTGA 4 cut(s) 616, 2263, 2533, 2560
AsuII TTCGAA 1 cut(s) 706
AvaII GGWCC 3 cut(s) 19, 1920, 2761
AvrII CCTAGG 1 cut(s) 1949
BaeGI GKGCMC 1 cut(s) 1832
BaeI ACNNNNGTAYC 2 cut(s) 686, 719
BbsI GAAGAC 3 cut(s) 462, 1137, 2603
BbvI GCAGC 2 cut(s) 1481, 2570
BccI CCATC 4 cut(s) 528, 1273, 1400, 2041
BceAI ACGGC 1 cut(s) 2238
BcgI CGANNNNNNTGC 4 cut(s) 1382, 1416, 1458, 1492
BciVI GTATCC 1 cut(s) 1891
BclI TGATCA 2 cut(s) 366, 714
BcnI CCSGG 2 cut(s) 1782, 1929
BcoDI GTCTC 3 cut(s) 192, 637, 1871
BcuI ACTAGT 1 cut(s) 796
BfmI CTRYAG 1 cut(s) 2535
BfoI RGCGCY 1 cut(s) 2033
BfuAI ACCTGC 1 cut(s) 65
BfuI GTATCC 1 cut(s) 1891
BglII AGATCT 1 cut(s) 1590
BisI GCNGC 3 cut(s) 775, 1470, 2584
BlnI CCTAGG 1 cut(s) 1949
BlpI GCTNAGC 1 cut(s) 1958
BlsI GCNGC 3 cut(s) 776, 1471, 2585
Bme1390I CCNGG 2 cut(s) 1782, 1929
Bme18I GGWCC 3 cut(s) 19, 1920, 2761
BmgBI CACGTC 1 cut(s) 844
BmgT120I GGNCC 6 cut(s) 19, 1920, 2611, 2644, 2676, 2761
BmiI GGNNCC 3 cut(s) 1922, 1997, 2762
BmrFI CCNGG 2 cut(s) 1782, 1929
BmrI ACTGGG 1 cut(s) 2001
BmsI GCATC 6 cut(s) 130, 950, 1481, 1491, 2142, 2541
BmuI ACTGGG 1 cut(s) 2001
BpiI GAAGAC 3 cut(s) 462, 1137, 2603
BplI GAGNNNNNCTC 2 cut(s) 1577, 1609
Bpu10I CCTNAGC 1 cut(s) 1433
Bpu1102I GCTNAGC 1 cut(s) 1958
Bpu14I TTCGAA 1 cut(s) 706
BpuEI CTTGAG 3 cut(s) 71, 262, 1828
BpuMI CCSGG 2 cut(s) 1782, 1929
BsaBI GATNNNNATC 1 cut(s) 1410
BsaJI CCNNGG 5 cut(s) 1927, 1949, 2517, 2661, 2706
BsaWI WCCGGW 1 cut(s) 2248
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 1 cut(s) 1090
Bse1I ACTGG 6 cut(s) 141, 501, 639, 1449, 1722, 1996
Bse3DI GCAATG 1 cut(s) 1279
Bse8I GATNNNNATC 1 cut(s) 1410
BseDI CCNNGG 5 cut(s) 1927, 1949, 2517, 2661, 2706
BseGI GGATG 9 cut(s) 145, 428, 539, 550, 941, 1214, 1341, 1765, 2750
BseJI GATNNNNATC 1 cut(s) 1410
BseLI CCNNNNNNNGG 1 cut(s) 1090
BseMI GCAATG 1 cut(s) 1279
BseMII CTCAG 5 cut(s) 1053, 1220, 1238, 1949, 2715
BseNI ACTGG 6 cut(s) 141, 501, 639, 1449, 1722, 1996
BseRI GAGGAG 3 cut(s) 122, 1601, 2259
BseSI GKGCMC 1 cut(s) 1832
BseXI GCAGC 2 cut(s) 1481, 2570
BsgI GTGCAG 1 cut(s) 1806
Bsh1236I CGCG 1 cut(s) 303
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 7 cut(s) 5, 149, 1107, 2613, 2646, 2677, 2777
BsiEI CGRYCG 1 cut(s) 271
BsiSI CCGG 3 cut(s) 1781, 1929, 2249
BslFI GGGAC 2 cut(s) 2035, 2105
BslI CCNNNNNNNGG 1 cut(s) 1090
BsmAI GTCTC 3 cut(s) 192, 637, 1871
BsmFI GGGAC 2 cut(s) 2035, 2105
BsmI GAATGC 2 cut(s) 769, 1028
BsnI GGCC 7 cut(s) 5, 149, 1107, 2613, 2646, 2677, 2777
Bsp119I TTCGAA 1 cut(s) 706
Bsp1286I GDGCHC 1 cut(s) 1832
Bsp143I GATC 6 cut(s) 366, 714, 1162, 1590, 2698, 2727
Bsp1720I GCTNAGC 1 cut(s) 1958
Bsp19I CCATGG 1 cut(s) 2661
BspACI CCGC 9 cut(s) 143, 267, 349, 464, 775, 1355, 1467, 1645, 1870
BspANI GGCC 7 cut(s) 5, 149, 1107, 2613, 2646, 2677, 2777
BspCNI CTCAG 5 cut(s) 1054, 1221, 1239, 1950, 2716
BspFNI CGCG 1 cut(s) 303
BspHI TCATGA 1 cut(s) 85
BspLI GGNNCC 3 cut(s) 1922, 1997, 2762
BspMI ACCTGC 1 cut(s) 65
BspPI GGATC 2 cut(s) 1170, 2706
BspT104I TTCGAA 1 cut(s) 706
BsrDI GCAATG 1 cut(s) 1279
BsrI ACTGG 6 cut(s) 141, 501, 639, 1449, 1722, 1996
BssECI CCNNGG 5 cut(s) 1927, 1949, 2517, 2661, 2706
BssMI GATC 6 cut(s) 366, 714, 1162, 1590, 2698, 2727
BssT1I CCWWGG 3 cut(s) 1949, 2661, 2706
Bst4CI ACNGT 6 cut(s) 401, 565, 977, 1687, 2602, 2748
Bst6I CTCTTC 4 cut(s) 1019, 1674, 2229, 2277
BstAPI GCANNNNNTGC 1 cut(s) 2059
BstBI TTCGAA 1 cut(s) 706
BstC8I GCNNGC 3 cut(s) 1081, 1524, 1964
BstDSI CCRYGG 1 cut(s) 2661
BstF5I GGATG 9 cut(s) 145, 428, 539, 550, 941, 1214, 1341, 1765, 2750
BstFNI CGCG 1 cut(s) 303
BstH2I RGCGCY 1 cut(s) 2033
BstHHI GCGC 3 cut(s) 305, 1968, 2032
BstKTI GATC 6 cut(s) 369, 717, 1165, 1593, 2701, 2730
BstMAI GTCTC 3 cut(s) 192, 637, 1871
BstMBI GATC 6 cut(s) 366, 714, 1162, 1590, 2698, 2727
BstMCI CGRYCG 1 cut(s) 271
BstMWI GCNNNNNNNGC 4 cut(s) 1434, 1963, 2059, 2222
BstNSI RCATGY 3 cut(s) 250, 1441, 2738
BstSCI CCNGG 2 cut(s) 1780, 1927
BstSFI CTRYAG 1 cut(s) 2535
BstSLI GKGCMC 1 cut(s) 1832
BstUI CGCG 1 cut(s) 303
BstV1I GCAGC 2 cut(s) 1481, 2570
BstV2I GAAGAC 3 cut(s) 462, 1137, 2603
BstX2I RGATCY 1 cut(s) 1590
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 1 cut(s) 1590
BsuI GTATCC 1 cut(s) 1891
BsuRI GGCC 7 cut(s) 5, 149, 1107, 2613, 2646, 2677, 2777
BtgI CCRYGG 1 cut(s) 2661
BtrI CACGTC 1 cut(s) 844
BtsCI GGATG 9 cut(s) 145, 428, 539, 550, 941, 1214, 1341, 1765, 2750
BtsI GCAGTG 1 cut(s) 2336
BtsIMutI CAGTG 5 cut(s) 1683, 1715, 1989, 2336, 2607
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 3 cut(s) 1081, 1524, 1964
CaiI CAGNNNCTG 1 cut(s) 988
CciI TCATGA 1 cut(s) 85
CfoI GCGC 3 cut(s) 305, 1968, 2032
Cfr13I GGNCC 6 cut(s) 19, 1920, 2611, 2644, 2676, 2761
Csp6I GTAC 6 cut(s) 55, 452, 695, 2002, 2566, 2711
CviQI GTAC 6 cut(s) 55, 452, 695, 2002, 2566, 2711
DpnI GATC 6 cut(s) 368, 716, 1164, 1592, 2700, 2729
DpnII GATC 6 cut(s) 366, 714, 1162, 1590, 2698, 2727
DraI TTTAAA 2 cut(s) 1324, 1818
Eam1104I CTCTTC 4 cut(s) 1019, 1674, 2229, 2277
EarI CTCTTC 4 cut(s) 1019, 1674, 2229, 2277
Eco130I CCWWGG 3 cut(s) 1949, 2661, 2706
Eco147I AGGCCT 1 cut(s) 1107
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 3 cut(s) 19, 1920, 2761
Eco57I CTGAAG 5 cut(s) 186, 1000, 1088, 1295, 1400
EcoO109I RGGNCCY 1 cut(s) 1920
EcoRI GAATTC 1 cut(s) 233
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 3 cut(s) 1949, 2661, 2706
EcoT22I ATGCAT 3 cut(s) 664, 1506, 2446
ErhI CCWWGG 3 cut(s) 1949, 2661, 2706
FalI AAGNNNNNCTT 2 cut(s) 1268, 1300
FaqI GGGAC 2 cut(s) 2035, 2105
FauI CCCGC 1 cut(s) 1638
FauNDI CATATG 1 cut(s) 2446
FbaI TGATCA 2 cut(s) 366, 714
FblI GTMKAC 2 cut(s) 271, 2101
Fnu4HI GCNGC 3 cut(s) 775, 1470, 2584
FokI GGATG 9 cut(s) 152, 415, 546, 557, 928, 1201, 1348, 1772, 2737
Fsp4HI GCNGC 3 cut(s) 775, 1470, 2584
FspI TGCGCA 1 cut(s) 1967
GlaI GCGC 3 cut(s) 304, 1967, 2031
GluI GCNGC 3 cut(s) 775, 1470, 2584
HaeII RGCGCY 1 cut(s) 2033
HaeIII GGCC 7 cut(s) 5, 149, 1107, 2613, 2646, 2677, 2777
HapII CCGG 3 cut(s) 1781, 1929, 2249
HhaI GCGC 3 cut(s) 305, 1968, 2032
Hin6I GCGC 3 cut(s) 303, 1966, 2030
HinP1I GCGC 3 cut(s) 303, 1966, 2030
HincII GTYRAC 4 cut(s) 272, 802, 1533, 2578
HindII GTYRAC 4 cut(s) 272, 802, 1533, 2578
HindIII AAGCTT 2 cut(s) 126, 2288
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 3 cut(s) 1781, 1929, 2249
HphI GGTGA 4 cut(s) 616, 2263, 2533, 2560
Hpy188I TCNGA 9 cut(s) 197, 433, 444, 1230, 1887, 2086, 2421, 2685, 2725
Hpy99I CGWCG 1 cut(s) 1605
HpyCH4III ACNGT 6 cut(s) 401, 565, 977, 1687, 2602, 2748
HpyCH4IV ACGT 3 cut(s) 843, 2209, 2316
HpyF10VI GCNNNNNNNGC 4 cut(s) 1434, 1963, 2059, 2222
HpySE526I ACGT 3 cut(s) 843, 2209, 2316
HspAI GCGC 3 cut(s) 303, 1966, 2030
Ksp22I TGATCA 2 cut(s) 366, 714
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 6 cut(s) 366, 714, 1162, 1590, 2698, 2727
LmnI GCTCC 2 cut(s) 310, 2708
Lsp1109I GCAGC 2 cut(s) 1481, 2570
LweI GCATC 6 cut(s) 130, 950, 1481, 1491, 2142, 2541
MaeII ACGT 3 cut(s) 843, 2209, 2316
MaeIII GTNAC 3 cut(s) 13, 1384, 1440
MalI GATC 6 cut(s) 368, 716, 1164, 1592, 2700, 2729
MboI GATC 6 cut(s) 366, 714, 1162, 1590, 2698, 2727
MflI RGATCY 1 cut(s) 1590
MhlI GDGCHC 1 cut(s) 1832
MlyI GAGTC 7 cut(s) 267, 476, 821, 918, 1738, 1919, 2113
MmeI TCCRAC 4 cut(s) 385, 969, 1082, 2478
Mph1103I ATGCAT 3 cut(s) 664, 1506, 2446
MroXI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2244
MslI CAYNNNNRTG 3 cut(s) 249, 1688, 2755
MspA1I CMGCKG 1 cut(s) 2583
MspI CCGG 3 cut(s) 1781, 1929, 2249
MspR9I CCNGG 2 cut(s) 1782, 1929
Mva1269I GAATGC 2 cut(s) 769, 1028
MvnI CGCG 1 cut(s) 303
MwoI GCNNNNNNNGC 4 cut(s) 1434, 1963, 2059, 2222
NciI CCSGG 2 cut(s) 1782, 1929
NcoI CCATGG 1 cut(s) 2661
NdeI CATATG 1 cut(s) 2446
NdeII GATC 6 cut(s) 366, 714, 1162, 1590, 2698, 2727
NlaIV GGNNCC 3 cut(s) 1922, 1997, 2762
NmeAIII GCCGAG 1 cut(s) 1095
NmuCI GTSAC 1 cut(s) 1384
NsbI TGCGCA 1 cut(s) 1967
NsiI ATGCAT 3 cut(s) 664, 1506, 2446
NspI RCATGY 3 cut(s) 250, 1441, 2738
NspV TTCGAA 1 cut(s) 706
PacI TTAATTAA 1 cut(s) 1839
PagI TCATGA 1 cut(s) 85
PaqCI CACCTGC 1 cut(s) 65
PceI AGGCCT 1 cut(s) 1107
PciI ACATGT 2 cut(s) 246, 2734
PctI GAATGC 2 cut(s) 769, 1028
PdmI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2244
PfeI GAWTC 3 cut(s) 192, 1728, 2081
PflMI CCANNNNNTGG 1 cut(s) 1090
PkrI GCNGC 3 cut(s) 776, 1471, 2585
PleI GAGTC 7 cut(s) 267, 475, 820, 917, 1738, 1918, 2113
PpsI GAGTC 7 cut(s) 267, 475, 820, 917, 1738, 1918, 2113
PpuMI RGGWCCY 1 cut(s) 1920
PscI ACATGT 2 cut(s) 246, 2734
PsiI TTATAA 1 cut(s) 2672
Psp5II RGGWCCY 1 cut(s) 1920
PspN4I GGNNCC 3 cut(s) 1922, 1997, 2762
PspPI GGNCC 6 cut(s) 19, 1920, 2611, 2644, 2676, 2761
PspPPI RGGWCCY 1 cut(s) 1920
PstNI CAGNNNCTG 1 cut(s) 988
PsuI RGATCY 1 cut(s) 1590
PvuII CAGCTG 1 cut(s) 2583
RsaI GTAC 6 cut(s) 56, 453, 696, 2003, 2567, 2712
RsaNI GTAC 6 cut(s) 55, 452, 695, 2002, 2566, 2711
RseI CAYNNNNRTG 3 cut(s) 249, 1688, 2755
SalI GTCGAC 1 cut(s) 270
SatI GCNGC 3 cut(s) 775, 1470, 2584
Sau3AI GATC 6 cut(s) 366, 714, 1162, 1590, 2698, 2727
Sau96I GGNCC 6 cut(s) 19, 1920, 2611, 2644, 2676, 2761
SchI GAGTC 7 cut(s) 267, 476, 821, 918, 1738, 1919, 2113
ScrFI CCNGG 2 cut(s) 1782, 1929
SduI GDGCHC 1 cut(s) 1832
SfaNI GCATC 6 cut(s) 130, 950, 1481, 1491, 2142, 2541
SfcI CTRYAG 1 cut(s) 2535
SfuI TTCGAA 1 cut(s) 706
SinI GGWCC 3 cut(s) 19, 1920, 2761
SmiI ATTTAAAT 1 cut(s) 1818
SmiMI CAYNNNNRTG 3 cut(s) 249, 1688, 2755
SmlI CTYRAG 3 cut(s) 50, 277, 1807
SmoI CTYRAG 3 cut(s) 50, 277, 1807
SpeI ACTAGT 1 cut(s) 796
SseBI AGGCCT 1 cut(s) 1107
SsiI CCGC 9 cut(s) 143, 267, 349, 464, 775, 1355, 1467, 1645, 1870
SspI AATATT 3 cut(s) 316, 2041, 2639
StuI AGGCCT 1 cut(s) 1107
StyD4I CCNGG 2 cut(s) 1780, 1927
StyI CCWWGG 3 cut(s) 1949, 2661, 2706
SwaI ATTTAAAT 1 cut(s) 1818
TaaI ACNGT 6 cut(s) 401, 565, 977, 1687, 2602, 2748
TaiI ACGT 3 cut(s) 846, 2212, 2319
TaqI TCGA 6 cut(s) 271, 706, 907, 925, 1726, 2144
TatI WGTACW 1 cut(s) 2001
TauI GCSGC 1 cut(s) 777
TfiI GAWTC 3 cut(s) 192, 1728, 2081
TscAI CASTG 5 cut(s) 1690, 1722, 1996, 2343, 2607
TseFI GTSAC 1 cut(s) 1384
TseI GCWGC 2 cut(s) 1469, 2583
Tsp45I GTSAC 1 cut(s) 1384
TspDTI ATGAA 9 cut(s) 102, 137, 226, 469, 645, 945, 1203, 1560, 2247
TspGWI ACGGA 2 cut(s) 32, 2459
TspRI CASTG 5 cut(s) 1690, 1722, 1996, 2343, 2607
Van91I CCANNNNNTGG 1 cut(s) 1090
VpaK11BI GGWCC 3 cut(s) 19, 1920, 2761
XapI RAATTY 7 cut(s) 233, 446, 757, 1493, 1819, 1932, 2509
XbaI TCTAGA 2 cut(s) 835, 1747
XceI RCATGY 3 cut(s) 250, 1441, 2738
XcmI CCANNNNNNNNNTGG 1 cut(s) 315
XmaJI CCTAGG 1 cut(s) 1949
XmiI GTMKAC 2 cut(s) 271, 2101
XmnI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2244
Zsp2I ATGCAT 3 cut(s) 664, 1506, 2446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.