MD10G1003800.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000110
Physical Location & Seq
Forward (+)
707511 .. 709540
2030 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1003800.v1.1.491

Sequence Viewer

Length: 1974 bp
ATGGCTTCACCTCCATTAGAGTTTTTGACGGGCAAGATAGTATCGATTATCGAAAATGAAGTGTCACTAATAGGAGGAGTTCGTGACCAACTCGATGAAATCAAGCGTGAGCTGCTAACCATGAAGGCTTTCCTGGCAGATGCTGGAAAGAAGGGAGCTCTCAGTGAAGTAGAGAAAACATGGGTGGAAAATGTGAGGGACGTATCCATCGATATCGAAGACATCATTGACGAGTTCACCTATCAAATGAACAGGCACAGAAGTTGGGGTCCATACACAAGAGCCTTTCGTCAAATGATTTGCTTCCCCAAGCATCTTTGGGAGAGGCATAGGATCGCCACCAAATTGCACAATATCATCAGAAAAATCAGAGCTATTCGAGAAAGAAACCAATGTTATGGCGTTGATCGCATACAAGGCATCGGTGCTTCACATAATTACGATCCCAATCGAGTCAGAATCTATGGTGAGTCCTCTCTTTTCTTTAAAGATGATGAACTTGTGGGAATCAAAGATGCTAAGGACAAGTTAGTTGGATGGTTGTTGTCTGAACAGCCAGAAAGAAGTGTAATTTCGGTGGTTGGAATGGGAGGTTCGGGCAAGACCACCTTAGTGGCAAACACCTTCAACAGTCAAACTGTAAAGAAACACTTTGATTGTCATGCTTGGATCACAGTCTCGCAAACTTACAACATCGAAGATCTGCTAAAGATAATGATCAAGGAACTTTTCAGAGCTGCAATGGAAGCTATTCCTCTTGACATAAACAATATGAGCTACAGAGGTTTGGTGGAAATGCTTATAAAATATATGCAGCCGAAGAGGTACGTGATTGTCTTGGATGATGTATGGGATAGAGAACTCTGGAGCCACATAAACGTAGCACTTCCTGATGGAGCACATGGAAGTCGAGTTGTGCTTACAACTCGGAACGAAGACATTGCTTCATTTTCATTCGGGGTCAGAAGCCATGTGTACCATGTTCATCCTCTCATTGAAAAAGAGGCTTGGGATCTTTTTTCCACGAAAGCTTTCTCTAGCTGGCCAGACAGGTCCTGCCCCCAAGAGCTCGAACACATTGCTAGGGATCTTGTTGGAAAGTGCAAAGGTTTACCCCTTGGAATTGTGGCTTTGGGTGCTCTCATGTCAACCAAAAGATTAAAGTCTGAATGGACAAGATTCAGCAGTAGCTTGAATTGGGAGCTAAGCAACAATCCAACACTTGAAGTAGTAAGGAGCATCCTACTGCTAAGCTTCAACGGTTTACCTTATCGACTCAAGCGTTGCTTCTTATATTTTTGCATTTTTCCAGAAGATTATGTGGTTAAATGTGAAAGGCTTAAAAGGCTGTGGATGGCAGAGGGGTTTGTCGAACAAGTTCGAGGGGCTACGCCTGAAGAGATTGCTGAAAGCTACATTGCAGAGCTCACTTGTCGATGCATGCTGCAAGTTGTTCAGAGGGAACCATCTGGAAGGGCAAAAGCATTCAAGGTGCATGACCTTTTGCGAGAACTTGCCCTTTCAATTTCAGAAGCTGAGAAATTTTGTGCAGTGTATCACAGTCAGGAAGCAAATGAAGGGTGTAAGGATTCACACCGGTTATCAGTGCGAGCAAGCAATGGAGAATTTCAACCACACAGAGATATGTCAAAGGTTCGTACCTTCTTTATTTTTTCCCCCGCGATGAGCGATTCTTCTTCACTAGAAAAACTGCCGTCCGGTTTCAAACTGTTAAGAGTCTTGGATCTAAAATATCTTCCGCTTGCTCAACTCCCAGACGATATAGTGAAGTGCTTTAACTTGAGGTATTTAAATCTGAAATCGACAAAAGTGAAAGAGCTTCCGAAAGACATAGGAAACCTCCATAATTTGGAAACATTAAACATTAAGAACTCCATGATAAGGTCACTTCCAGTTGGAATGGTGAAGTTAAAAAACCTACGACATCTTGTGATCAAGGTTCGACATAAGTAA

Protein Analysis

658

Amino Acids

75.46

Weight (kDa)

9.03

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 89 1.3e-23 Rx N-terminal domain
NB-ARC PF00931 171 - 346 2.3e-47 NB-ARC domain
WHD_DRP PF23559 435 - 506 3.1e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 551 - 654 8.4e-22 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 803
AccB7I CCANNNNNTGG 1 cut(s) 1870
AccII CGCG 1 cut(s) 1682
AciI CCGC 2 cut(s) 1680, 1760
AclWI GGATC 6 cut(s) 341, 437, 677, 1020, 1095, 1752
AcoI YGGCCR 1 cut(s) 1043
AcsI RAATTY 2 cut(s) 1541, 1625
AcuI CTGAAG 1 cut(s) 1416
AfaI GTAC 3 cut(s) 827, 977, 1660
AfiI CCNNNNNNNGG 2 cut(s) 1870, 1902
AgeI ACCGGT 1 cut(s) 1596
AgsI TTSAA 9 cut(s) 628, 998, 1195, 1226, 1258, 1489, 1524, 1631, 1726
AjnI CCWGG 1 cut(s) 132
AleI CACNNNNGTG 1 cut(s) 611
Alw21I GWGCWC 5 cut(s) 160, 901, 1071, 1141, 1428
Alw26I GTCTC 1 cut(s) 682
AlwI GGATC 6 cut(s) 341, 437, 677, 1020, 1095, 1752
AlwNI CAGNNNCTG 3 cut(s) 143, 1056, 1535
AoxI GGCC 1 cut(s) 1043
ApeKI GCWGC 4 cut(s) 112, 737, 814, 1444
ApoI RAATTY 2 cut(s) 1541, 1625
AsiGI ACCGGT 1 cut(s) 1596
Asp700I GAANNNNTTC 4 cut(s) 128, 750, 1031, 1377
AspS9I GGNCC 2 cut(s) 269, 1053
AsuHPI GGTGA 3 cut(s) 229, 479, 1936
AvaII GGWCC 2 cut(s) 269, 1053
BalI TGGCCA 1 cut(s) 1045
BanII GRGCYC 3 cut(s) 160, 1071, 1428
BbsI GAAGAC 2 cut(s) 225, 942
Bbv12I GWGCWC 5 cut(s) 160, 901, 1071, 1141, 1428
BbvI GCAGC 4 cut(s) 99, 724, 826, 1431
BccI CCATC 5 cut(s) 215, 531, 887, 1348, 1474
BceAI ACGGC 1 cut(s) 1699
BcgI CGANNNNNNTGC 4 cut(s) 923, 957, 1061, 1095
BciT130I CCWGG 1 cut(s) 134
BciVI GTATCC 1 cut(s) 214
BclI TGATCA 2 cut(s) 717, 1953
BcoDI GTCTC 1 cut(s) 682
BfaI CTAG 3 cut(s) 1038, 1083, 1703
BfmI CTRYAG 1 cut(s) 778
BfuI GTATCC 1 cut(s) 214
BglII AGATCT 1 cut(s) 700
BisI GCNGC 4 cut(s) 113, 738, 815, 1445
BlpI GCTNAGC 2 cut(s) 1205, 1250
BlsI GCNGC 4 cut(s) 114, 739, 816, 1446
Bme1390I CCNGG 1 cut(s) 134
Bme18I GGWCC 2 cut(s) 269, 1053
BmgT120I GGNCC 2 cut(s) 269, 1053
BmiI GGNNCC 3 cut(s) 270, 869, 1464
BmrFI CCNGG 1 cut(s) 134
BmsI GCATC 6 cut(s) 130, 322, 429, 505, 1248, 1427
BpiI GAAGAC 2 cut(s) 225, 942
BpmI CTGGAG 1 cut(s) 886
Bpu10I CCTNAGC 1 cut(s) 519
Bpu1102I GCTNAGC 2 cut(s) 1205, 1250
BpuEI CTTGAG 2 cut(s) 1262, 1822
Bsa29I ATCGAT 2 cut(s) 44, 210
BsaAI YACGTR 1 cut(s) 829
BsaBI GATNNNNATC 2 cut(s) 447, 716
BsaJI CCNNGG 1 cut(s) 1117
BsaWI WCCGGW 2 cut(s) 1596, 1718
Bsc4I CCNNNNNNNGG 2 cut(s) 1870, 1902
Bse118I RCCGGY 1 cut(s) 1596
Bse1I ACTGG 1 cut(s) 1913
Bse3DI GCAATG 5 cut(s) 747, 939, 1077, 1416, 1624
Bse8I GATNNNNATC 2 cut(s) 447, 716
BseBI CCWGG 1 cut(s) 134
BseCI ATCGAT 2 cut(s) 44, 210
BseDI CCNNGG 1 cut(s) 1117
BseGI GGATG 5 cut(s) 542, 847, 985, 1239, 1359
BseJI GATNNNNATC 2 cut(s) 447, 716
BseLI CCNNNNNNNGG 2 cut(s) 1870, 1902
BseMI GCAATG 5 cut(s) 747, 939, 1077, 1416, 1624
BseMII CTCAG 2 cut(s) 175, 1527
BseNI ACTGG 1 cut(s) 1913
BseRI GAGGAG 1 cut(s) 90
BseXI GCAGC 4 cut(s) 99, 724, 826, 1431
BsgI GTGCAG 1 cut(s) 1569
Bsh1236I CGCG 1 cut(s) 1682
BshFI GGCC 1 cut(s) 1045
BshTI ACCGGT 1 cut(s) 1596
BshVI ATCGAT 2 cut(s) 44, 210
BsiHKAI GWGCWC 5 cut(s) 160, 901, 1071, 1141, 1428
BsiSI CCGG 2 cut(s) 1597, 1719
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 2 cut(s) 1870, 1902
BsmAI GTCTC 1 cut(s) 682
BsmFI GGGAC 1 cut(s) 212
BsmI GAATGC 1 cut(s) 1484
BsnI GGCC 1 cut(s) 1045
Bsp1286I GDGCHC 5 cut(s) 160, 901, 1071, 1141, 1428
Bsp1720I GCTNAGC 2 cut(s) 1205, 1250
BspACI CCGC 2 cut(s) 1680, 1760
BspANI GGCC 1 cut(s) 1045
BspCNI CTCAG 2 cut(s) 174, 1528
BspDI ATCGAT 2 cut(s) 44, 210
BspFNI CGCG 1 cut(s) 1682
BspLI GGNNCC 3 cut(s) 270, 869, 1464
BspPI GGATC 6 cut(s) 341, 437, 677, 1020, 1095, 1752
BsrDI GCAATG 5 cut(s) 747, 939, 1077, 1416, 1624
BsrFI RCCGGY 1 cut(s) 1596
BsrI ACTGG 1 cut(s) 1913
BssAI RCCGGY 1 cut(s) 1596
BssECI CCNNGG 1 cut(s) 1117
BssT1I CCWWGG 1 cut(s) 1117
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 6 cut(s) 632, 640, 676, 1262, 1562, 1731
Bst6I CTCTTC 2 cut(s) 815, 1392
BstBAI YACGTR 1 cut(s) 829
BstC8I GCNNGC 5 cut(s) 1043, 1442, 1611, 1615, 1764
BstDEI CTNAG 6 cut(s) 161, 519, 610, 1205, 1250, 1536
BstF5I GGATG 5 cut(s) 542, 847, 985, 1239, 1359
BstFNI CGCG 1 cut(s) 1682
BstMAI GTCTC 1 cut(s) 682
BstMWI GCNNNNNNNGC 7 cut(s) 112, 134, 408, 417, 746, 1136, 1345
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 1 cut(s) 1444
BstSCI CCNGG 1 cut(s) 132
BstSFI CTRYAG 1 cut(s) 778
BstUI CGCG 1 cut(s) 1682
BstV1I GCAGC 4 cut(s) 99, 724, 826, 1431
BstV2I GAAGAC 2 cut(s) 225, 942
BstX2I RGATCY 4 cut(s) 700, 1012, 1087, 1744
BstXI CCANNNNNNTGG 2 cut(s) 398, 613
BstYI RGATCY 4 cut(s) 700, 1012, 1087, 1744
Bsu15I ATCGAT 2 cut(s) 44, 210
BsuI GTATCC 1 cut(s) 214
BsuRI GGCC 1 cut(s) 1045
BsuTUI ATCGAT 2 cut(s) 44, 210
BtgZI GCGATG 1 cut(s) 1697
BtsCI GGATG 5 cut(s) 542, 847, 985, 1239, 1359
BtsI GCAGTG 1 cut(s) 1557
BtsIMutI CAGTG 3 cut(s) 169, 1557, 1611
Cac8I GCNNGC 5 cut(s) 1043, 1442, 1611, 1615, 1764
CaiI CAGNNNCTG 3 cut(s) 143, 1056, 1535
Cfr10I RCCGGY 1 cut(s) 1596
Cfr13I GGNCC 2 cut(s) 269, 1053
ClaI ATCGAT 2 cut(s) 44, 210
Csp6I GTAC 3 cut(s) 826, 976, 1659
CspAI ACCGGT 1 cut(s) 1596
CviQI GTAC 3 cut(s) 826, 976, 1659
DdeI CTNAG 6 cut(s) 161, 519, 610, 1205, 1250, 1536
DraI TTTAAA 2 cut(s) 487, 1812
EaeI YGGCCR 1 cut(s) 1043
Eam1104I CTCTTC 2 cut(s) 815, 1392
EarI CTCTTC 2 cut(s) 815, 1392
Ecl136II GAGCTC 3 cut(s) 158, 1069, 1426
Eco130I CCWWGG 1 cut(s) 1117
Eco24I GRGCYC 3 cut(s) 160, 1071, 1428
Eco32I GATATC 1 cut(s) 214
Eco47I GGWCC 2 cut(s) 269, 1053
Eco53kI GAGCTC 3 cut(s) 158, 1069, 1426
Eco57I CTGAAG 1 cut(s) 1416
EcoICRI GAGCTC 3 cut(s) 158, 1069, 1426
EcoO109I RGGNCCY 1 cut(s) 1053
EcoRII CCWGG 1 cut(s) 132
EcoRV GATATC 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 1117
EcoT22I ATGCAT 1 cut(s) 1442
EcoT38I GRGCYC 3 cut(s) 160, 1071, 1428
ErhI CCWWGG 1 cut(s) 1117
FalI AAGNNNNNCTT 6 cut(s) 593, 625, 635, 667, 1271, 1303
FaqI GGGAC 1 cut(s) 212
FauI CCCGC 1 cut(s) 1687
FbaI TGATCA 2 cut(s) 717, 1953
Fnu4HI GCNGC 4 cut(s) 113, 738, 815, 1445
FokI GGATG 5 cut(s) 549, 854, 972, 1226, 1366
FriOI GRGCYC 3 cut(s) 160, 1071, 1428
Fsp4HI GCNGC 4 cut(s) 113, 738, 815, 1445
FspBI CTAG 3 cut(s) 1038, 1083, 1703
GluI GCNGC 4 cut(s) 113, 738, 815, 1445
GsuI CTGGAG 1 cut(s) 886
HaeIII GGCC 1 cut(s) 1045
HapII CCGG 2 cut(s) 1597, 1719
HincII GTYRAC 1 cut(s) 1149
HindII GTYRAC 1 cut(s) 1149
HindIII AAGCTT 2 cut(s) 1029, 1252
HinfI GANTC 9 cut(s) 453, 459, 470, 507, 1179, 1275, 1589, 1691, 1737
HpaII CCGG 2 cut(s) 1597, 1719
HphI GGTGA 3 cut(s) 229, 479, 1936
Hpy166II GTNNAC 5 cut(s) 237, 976, 1112, 1149, 1265
Hpy188III TCNNGA 8 cut(s) 83, 380, 758, 865, 890, 1310, 1470, 1565
Hpy8I GTNNAC 5 cut(s) 237, 976, 1112, 1149, 1265
HpyAV CCTTC 6 cut(s) 118, 145, 634, 1467, 1571, 1672
HpyCH4III ACNGT 6 cut(s) 632, 640, 676, 1262, 1562, 1731
HpyCH4IV ACGT 3 cut(s) 201, 828, 879
HpyF10VI GCNNNNNNNGC 7 cut(s) 112, 134, 408, 417, 746, 1136, 1345
HpyF3I CTNAG 6 cut(s) 161, 519, 610, 1205, 1250, 1536
HpySE526I ACGT 3 cut(s) 201, 828, 879
Ksp22I TGATCA 2 cut(s) 717, 1953
LmnI GCTCC 5 cut(s) 155, 867, 896, 1201, 1236
Lsp1109I GCAGC 4 cut(s) 99, 724, 826, 1431
LweI GCATC 6 cut(s) 130, 322, 429, 505, 1248, 1427
MaeI CTAG 3 cut(s) 1038, 1083, 1703
MaeII ACGT 3 cut(s) 201, 828, 879
MaeIII GTNAC 3 cut(s) 63, 83, 1905
MboII GAAGA 9 cut(s) 230, 710, 832, 947, 1325, 1409, 1686, 1689, 1748
MflI RGATCY 4 cut(s) 700, 1012, 1087, 1744
MhlI GDGCHC 5 cut(s) 160, 901, 1071, 1141, 1428
MlsI TGGCCA 1 cut(s) 1045
MluCI AATT 9 cut(s) 344, 436, 570, 1122, 1195, 1524, 1541, 1625, 1867
MluNI TGGCCA 1 cut(s) 1045
MlyI GAGTC 4 cut(s) 462, 479, 1269, 1746
MmeI TCCRAC 5 cut(s) 514, 562, 1075, 1241, 1897
Mox20I TGGCCA 1 cut(s) 1045
Mph1103I ATGCAT 1 cut(s) 1442
MroXI GAANNNNTTC 4 cut(s) 128, 750, 1031, 1377
MscI TGGCCA 1 cut(s) 1045
MslI CAYNNNNRTG 1 cut(s) 611
Msp20I TGGCCA 1 cut(s) 1045
MspI CCGG 2 cut(s) 1597, 1719
MspR9I CCNGG 1 cut(s) 134
Mva1269I GAATGC 1 cut(s) 1484
MvaI CCWGG 1 cut(s) 134
MvnI CGCG 1 cut(s) 1682
MwoI GCNNNNNNNGC 7 cut(s) 112, 134, 408, 417, 746, 1136, 1345
NlaIV GGNNCC 3 cut(s) 270, 869, 1464
NmuCI GTSAC 3 cut(s) 63, 83, 1905
NsiI ATGCAT 1 cut(s) 1442
NspI RCATGY 1 cut(s) 1444
OliI CACNNNNGTG 1 cut(s) 611
PaeI GCATGC 1 cut(s) 1444
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 1279
PctI GAATGC 1 cut(s) 1484
PdmI GAANNNNTTC 4 cut(s) 128, 750, 1031, 1377
PfeI GAWTC 5 cut(s) 459, 507, 1179, 1589, 1691
PflMI CCANNNNNTGG 1 cut(s) 1870
PinAI ACCGGT 1 cut(s) 1596
PkrI GCNGC 4 cut(s) 114, 739, 816, 1446
PleI GAGTC 4 cut(s) 461, 478, 1269, 1745
PpsI GAGTC 4 cut(s) 461, 478, 1269, 1745
Ppu21I YACGTR 1 cut(s) 829
PpuMI RGGWCCY 1 cut(s) 1053
PsiI TTATAA 1 cut(s) 803
Psp124BI GAGCTC 3 cut(s) 160, 1071, 1428
Psp5II RGGWCCY 1 cut(s) 1053
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 3 cut(s) 270, 869, 1464
PspPI GGNCC 2 cut(s) 269, 1053
PspPPI RGGWCCY 1 cut(s) 1053
PstNI CAGNNNCTG 3 cut(s) 143, 1056, 1535
PsuI RGATCY 4 cut(s) 700, 1012, 1087, 1744
RsaI GTAC 3 cut(s) 827, 977, 1660
RsaNI GTAC 3 cut(s) 826, 976, 1659
RseI CAYNNNNRTG 1 cut(s) 611
SacI GAGCTC 3 cut(s) 160, 1071, 1428
SatI GCNGC 4 cut(s) 113, 738, 815, 1445
Sau96I GGNCC 2 cut(s) 269, 1053
SchI GAGTC 4 cut(s) 462, 479, 1269, 1746
ScrFI CCNGG 1 cut(s) 134
SduI GDGCHC 5 cut(s) 160, 901, 1071, 1141, 1428
SfaNI GCATC 6 cut(s) 130, 322, 429, 505, 1248, 1427
SfcI CTRYAG 1 cut(s) 778
SinI GGWCC 2 cut(s) 269, 1053
SmiI ATTTAAAT 1 cut(s) 1812
SmiMI CAYNNNNRTG 1 cut(s) 611
SmlI CTYRAG 2 cut(s) 1277, 1801
SmoI CTYRAG 2 cut(s) 1277, 1801
SphI GCATGC 1 cut(s) 1444
Sse9I AATT 9 cut(s) 344, 436, 570, 1122, 1195, 1524, 1541, 1625, 1867
SsiI CCGC 2 cut(s) 1680, 1760
SspMI CTAG 3 cut(s) 1038, 1083, 1703
SstI GAGCTC 3 cut(s) 160, 1071, 1428
StyD4I CCNGG 1 cut(s) 132
StyI CCWWGG 1 cut(s) 1117
SwaI ATTTAAAT 1 cut(s) 1812
TaaI ACNGT 6 cut(s) 632, 640, 676, 1262, 1562, 1731
TaiI ACGT 3 cut(s) 204, 831, 882
TasI AATT 9 cut(s) 344, 436, 570, 1122, 1195, 1524, 1541, 1625, 1867
TfiI GAWTC 5 cut(s) 459, 507, 1179, 1589, 1691
TscAI CASTG 3 cut(s) 169, 1557, 1611
TseFI GTSAC 3 cut(s) 63, 83, 1905
TseI GCWGC 4 cut(s) 112, 737, 814, 1444
Tsp45I GTSAC 3 cut(s) 63, 83, 1905
TspDTI ATGAA 9 cut(s) 72, 111, 137, 263, 510, 936, 942, 974, 1590
TspRI CASTG 3 cut(s) 169, 1557, 1611
Van91I CCANNNNNTGG 1 cut(s) 1870
VpaK11BI GGWCC 2 cut(s) 269, 1053
XapI RAATTY 2 cut(s) 1541, 1625
XceI RCATGY 1 cut(s) 1444
XmnI GAANNNNTTC 4 cut(s) 128, 750, 1031, 1377
XspI CTAG 3 cut(s) 1038, 1083, 1703
Zsp2I ATGCAT 1 cut(s) 1442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.