Rw5G011920

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000110
Physical Location & Seq
Forward (+)
13826227 .. 13828977
2751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G011920.1

Sequence Viewer

Length: 2688 bp
ATGGCCTCATCTGTAACAGACCTCTTAATTGGAAAAATTGTGGCAATTCTTGAGAACGAAGGAACAACCATAGCCAGTGTTCGTGATGAAGTTGATAGCATTAAGGAGGAGCTTCGAAGCATGAAAGCTTTCCTAGTAGATGCGGAGGCCGACACAGCAAAAACTGAAACAGAGAAATTGCGGGTTGAAAGCATCAGAGACTTGGCCTATGATGTTGAAGATATCATTGATGAATTCATGTATCACATGTATGAGAGGCAAAGTGGGAGTCGACTTTCAAGGAGGCTACACAAAACCATTTGCTTTCCAAAAAGTCTTTGGTTTAGACGTAAAACTGCAAAGAGGTTACAGAAAATCACTAAAAAGATCAGAGACATTCCAGAGAGGAATCAAAGGTACACGGACTCTTCGTTTTTCAGTATGGAAGACGAACTGGTTGGGATTGAAGGCAAGAAGCAAATACTAATGGGATGGCTCATGGATGAAGAGCAACACCAAACTGTTATATCTGTGGTTGGCATGGGAGGTTCTGGAAAGACAACTCTAGCTGCCAAGACCTTCAACAATGAAAAGGTGAAGAGACATTTTGATTGTTGTGCGTGGATTACCGTTTCCCAAACTTCTGATGTGGTACACTTGTTTCGAAGCTTGATCATGGAACTCTACAAATCAAAAAAGGAAGATGTCCCTGAAAATTTGAATACCATGAGCAGCAGAGAATTGATAGGGTTACTAGTTCACTACTTGGAGTCCAAAAGATACCTGGTTGTTCTAGATGACGTGTGGGATAATAAAGATTGGAGCGAAATAAAGGTATCACTTCAAGATAGGCAGCTCCGAAGTCGAATCATAGTTACAACTCGAAAAGAAGATGTAGCATGCAATTCTTTTGGAGTTAAAAGCCATGTTCACCATATTCAGCCCCTACAAAAGGATGAGGCTTGGGAGCTCTTCTGCAAAAAAGCATTCTCATCTGATGAGCACAAAACTTGTCCTCCTGATTTTGTGTCATTAGCTTGCCAACTGGTGGAAAAGTGTGAAGGCCTTCCTTTGGCACTTGTGGCTTTAGGTGGTCTTATGTCTTCCAAGAAGTCCCTGGATCAATGGCAACAAGTCTGCAACAGCTTGAATTGGCATCTAAATAAGCTAGACCGTGTGAAGAAAATCTTGTTGCTGAGTTTCAATGATTTGCCAGCTCAATTGAAGCATTGCTTCCTCTATTGTTCTCTTTTCCCAGAAGATTTTTCAATCAACAGAAAAAGGCTTATCAAATTGTGGATAGCTGAAGGATTCGTTGAGCATGTGGAAGGGATCACGCTCGAAGAAGTTGCAGATGGCTATCTTTGGAAACTCTGTTTCTGTAGCATGTTACAAGTTGTTGTGAGAAATGAAACAGGAAGGTCGAGACATGTTAAGATGCATGATCTGATGCGAGAGTTTGCTTTGTCGACAGCTGAGAAGGAAAAGTTTGGTCTTGCATACGACGGGAGACAAGTAACGGAAGACATCTCAACCCGTCGCTTGTCAATTCACAAAATGAGTAATGGAGGAGAAATTGAATCATGGCCGGGTATGTCAAAGGTTCGTGCTCTTCTTGTCTTTGCAACTGACATGTCCTCATTGTCTTTCTCAAATGCACTGGTTTCTCGATTCAAATTGTTGAGGACTCTAGACTTGGAGGATGTACAAATTGATAAACTGCCAGATGCAGTTGTTTACTTGTTCAACTTGAGATACTTAAATCTGAAGGGCACTTTAATCGAGGAACTTCCAGAGTCCATAGGGCGTCTGCGCAATCTTCAATTTCTGAACATCAAGGGGACTAAGATAGAGTCACTTCCACAAGGAATTACGAAGTTGCTAAACCTGCGCCATATAATTATGTATAGCTACACTGGAAACTATATGGACTTCAAATTTATGAGGGGGACAAGAGCGCCATCAAATATTTCCAAATTCCAGAAATTGCAATATTTATCATTGATTGAATCAGAAGGGAACACTATTAGACTTATCGGCAACCTGACTCAAATAACGAACATCGGCATCTCGAATGTGAAAGAAAGAGATGGGATTGACCTTTGGGCCTCAATTAAGAAGTTGAATCTGCTTCGAACTATCACTTTAATGGCAAGTAATGAAGAGGAAATTCTTCCGGTCCAAGCACAATGTCCACATCTTCCGCACCTTCAAAAGCTTGATTTGATTGGCAGGCTGCGAAACGTAATACCTTGGTTTTCTTCACTGCATAGCCTAACAACTCTGTATCTGCGTTGGTCAAGACTTGAAGAGGATTTACTGCCTCAAATTGAAGAACTGCCCAATCTGACAATGCTTCATCTTTGGAACGCATATGTTGGGGAAGAGTTGTGTTTCCGCACAGGCTTTGTAAAGCTGATAGAGCTGCAGTTGTTCAATTTTTCCTCGGTGAAAAAGATAACTATAGAAAAAGGGACGATGCCAAATCTCGAGAAGTTATGGGTTGACAGCTGCATGGAGTTGAAGATAGTACCACTGGGCCTTGAATACATTTCTAATCTACAAATTTTGATCCTGGCAATTGTTCCAATAGAAATTATACAGCCGATTCGGAAAGGAGGTGTTGATCGGTCTAAGGTACAACACATCCCTGAGATCAGACATATTTTCCAACAATCATCTGAGCGGTTCTACGAAAACTTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

895

Amino Acids

103.21

Weight (kDa)

8.16

Isoelectric Point (pI)

56.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 94 7.5e-21 Rx N-terminal domain
NB-ARC PF00931 151 - 326 8.3e-50 NB-ARC domain
WHD_DRP PF23559 410 - 481 3e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 527 - 849 7.2e-40 Leucine-rich repeat region
LRR_8 PF13855 554 - 610 1.6e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1793
Acc36I ACCTGC 1 cut(s) 1875
AccB7I CCANNNNNTGG 1 cut(s) 1027
AccBSI CCGCTC 1 cut(s) 2665
AccI GTMKAC 2 cut(s) 271, 1448
AciI CCGC 5 cut(s) 143, 181, 2183, 2377, 2665
AclWI GGATC 3 cut(s) 1107, 1319, 2545
AcoI YGGCCR 1 cut(s) 1565
AcsI RAATTY 6 cut(s) 233, 694, 1916, 1955, 2148, 2544
AcuI CTGAAG 2 cut(s) 1305, 1766
AcyI GRCGYC 1 cut(s) 1786
AfaI GTAC 5 cut(s) 398, 633, 1686, 2511, 2619
AfiI CCNNNNNNNGG 3 cut(s) 931, 1027, 1051
AflIII ACRYGT 4 cut(s) 246, 780, 1408, 1611
AhlI ACTAGT 1 cut(s) 733
AjiI CACGTC 1 cut(s) 781
AjnI CCWGG 3 cut(s) 762, 1095, 2553
Alw21I GWGCWC 3 cut(s) 951, 984, 1591
Alw26I GTCTC 5 cut(s) 192, 366, 574, 1399, 1483
AlwI GGATC 3 cut(s) 1107, 1319, 2545
Ama87I CYCGRG 1 cut(s) 2468
AoxI GGCC 8 cut(s) 3, 147, 204, 1042, 1565, 2085, 2518, 2682
ApeKI GCWGC 6 cut(s) 548, 711, 832, 2215, 2404, 2490
ApoI RAATTY 6 cut(s) 233, 694, 1916, 1955, 2148, 2544
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 3 cut(s) 128, 1044, 2151
AspLEI GCGC 3 cut(s) 1794, 1872, 1939
AspS9I GGNCC 3 cut(s) 2085, 2158, 2518
AsuC2I CCSGG 1 cut(s) 1569
AsuHPI GGTGA 3 cut(s) 586, 902, 2440
AsuII TTCGAA 3 cut(s) 115, 643, 2113
AvaI CYCGRG 1 cut(s) 2468
AvaII GGWCC 1 cut(s) 2158
BaeGI GKGCMC 1 cut(s) 1754
BaeI ACNNNNGTAYC 2 cut(s) 623, 656
BanII GRGCYC 1 cut(s) 951
BarI GAAGNNNNNNTAC 2 cut(s) 2280, 2312
BbsI GAAGAC 3 cut(s) 432, 1074, 1509
Bbv12I GWGCWC 3 cut(s) 951, 984, 1591
BbvI GCAGC 6 cut(s) 535, 723, 844, 2202, 2391, 2477
BccI CCATC 4 cut(s) 465, 1328, 1948, 2063
BcgI CGANNNNNNTGC 4 cut(s) 1310, 1344, 1741, 1775
BciT130I CCWGG 3 cut(s) 764, 1097, 2555
BclI TGATCA 1 cut(s) 651
BcnI CCSGG 1 cut(s) 1569
BcoDI GTCTC 5 cut(s) 192, 366, 574, 1399, 1483
BcuI ACTAGT 1 cut(s) 733
BfaI CTAG 7 cut(s) 134, 545, 734, 773, 1148, 1670, 2686
BfmI CTRYAG 3 cut(s) 1360, 2405, 2442
BfoI RGCGCY 1 cut(s) 1940
BfuAI ACCTGC 1 cut(s) 1875
BisI GCNGC 6 cut(s) 549, 712, 833, 2216, 2405, 2491
BlsI GCNGC 6 cut(s) 550, 713, 834, 2217, 2406, 2492
Bme1390I CCNGG 4 cut(s) 764, 1097, 1569, 2555
Bme18I GGWCC 1 cut(s) 2158
BmeT110I CYCGRG 1 cut(s) 2468
BmgBI CACGTC 1 cut(s) 781
BmgT120I GGNCC 3 cut(s) 2085, 2158, 2518
BmrFI CCNGG 4 cut(s) 764, 1097, 1569, 2555
BmrI ACTGGG 1 cut(s) 2525
BmsI GCATC 8 cut(s) 130, 201, 1144, 1407, 1419, 1696, 2055, 2448
BmuI ACTGGG 1 cut(s) 2525
BpiI GAAGAC 3 cut(s) 432, 1074, 1509
Bpu14I TTCGAA 3 cut(s) 115, 643, 2113
BpuEI CTTGAG 2 cut(s) 71, 1750
BpuMI CCSGG 1 cut(s) 1569
BsaBI GATNNNNATC 1 cut(s) 1338
BsaHI GRCGYC 1 cut(s) 1786
BsaJI CCNNGG 3 cut(s) 1095, 2231, 2424
BsaWI WCCGGW 1 cut(s) 2155
BsaXI ACNNNNNCTCC 2 cut(s) 979, 1009
Bsc4I CCNNNNNNNGG 3 cut(s) 931, 1027, 1051
Bse1I ACTGG 6 cut(s) 75, 438, 1029, 1644, 1900, 2520
Bse3DI GCAATG 1 cut(s) 1207
Bse8I GATNNNNATC 1 cut(s) 1338
BseBI CCWGG 3 cut(s) 764, 1097, 2555
BseDI CCNNGG 3 cut(s) 1095, 2231, 2424
BseGI GGATG 5 cut(s) 476, 487, 940, 1687, 2625
BseJI GATNNNNATC 1 cut(s) 1338
BseLI CCNNNNNNNGG 3 cut(s) 931, 1027, 1051
BseMI GCAATG 1 cut(s) 1207
BseMII CTCAG 4 cut(s) 1166, 1446, 2622, 2652
BseNI ACTGG 6 cut(s) 75, 438, 1029, 1644, 1900, 2520
BseRI GAGGAG 2 cut(s) 122, 1563
BseSI GKGCMC 1 cut(s) 1754
BseXI GCAGC 6 cut(s) 535, 723, 844, 2202, 2391, 2477
BshFI GGCC 8 cut(s) 5, 149, 206, 1044, 1567, 2087, 2520, 2684
BsiHKAI GWGCWC 3 cut(s) 951, 984, 1591
BsiHKCI CYCGRG 1 cut(s) 2468
BsiSI CCGG 2 cut(s) 1568, 2156
BslFI GGGAC 5 cut(s) 671, 1078, 1834, 1942, 2467
BslI CCNNNNNNNGG 3 cut(s) 931, 1027, 1051
BsmAI GTCTC 5 cut(s) 192, 366, 574, 1399, 1483
BsmFI GGGAC 5 cut(s) 671, 1078, 1834, 1942, 2467
BsmI GAATGC 1 cut(s) 965
BsnI GGCC 8 cut(s) 5, 149, 206, 1044, 1567, 2087, 2520, 2684
BsoBI CYCGRG 1 cut(s) 2468
Bsp119I TTCGAA 3 cut(s) 115, 643, 2113
Bsp1286I GDGCHC 4 cut(s) 951, 984, 1591, 1754
Bsp1407I TGTACA 1 cut(s) 1684
Bsp143I GATC 8 cut(s) 366, 651, 1099, 1311, 1423, 2550, 2605, 2634
BspACI CCGC 5 cut(s) 143, 181, 2183, 2377, 2665
BspANI GGCC 8 cut(s) 5, 149, 206, 1044, 1567, 2087, 2520, 2684
BspCNI CTCAG 4 cut(s) 1167, 1447, 2623, 2653
BspMAI CTGCAG 1 cut(s) 2409
BspMI ACCTGC 1 cut(s) 1875
BspPI GGATC 3 cut(s) 1107, 1319, 2545
BspQI GCTCTTC 3 cut(s) 480, 956, 1596
BspT104I TTCGAA 3 cut(s) 115, 643, 2113
BsrBI CCGCTC 1 cut(s) 2665
BsrDI GCAATG 1 cut(s) 1207
BsrGI TGTACA 1 cut(s) 1684
BsrI ACTGG 6 cut(s) 75, 438, 1029, 1644, 1900, 2520
BssECI CCNNGG 3 cut(s) 1095, 2231, 2424
BssMI GATC 8 cut(s) 366, 651, 1099, 1311, 1423, 2550, 2605, 2634
BssNI GRCGYC 1 cut(s) 1786
BssT1I CCWWGG 1 cut(s) 2231
Bst2UI CCWGG 3 cut(s) 764, 1097, 2555
Bst4CI ACNGT 3 cut(s) 502, 610, 1154
Bst6I CTCTTC 8 cut(s) 412, 480, 572, 956, 1596, 2136, 2283, 2358
BstACI GRCGYC 1 cut(s) 1786
BstAUI TGTACA 1 cut(s) 1684
BstBI TTCGAA 3 cut(s) 115, 643, 2113
BstC8I GCNNGC 4 cut(s) 880, 1018, 1194, 2213
BstDEI CTNAG 6 cut(s) 1175, 1455, 1824, 2613, 2631, 2661
BstENI CCTNNNNNAGG 1 cut(s) 929
BstF5I GGATG 5 cut(s) 476, 487, 940, 1687, 2625
BstH2I RGCGCY 1 cut(s) 1940
BstHHI GCGC 3 cut(s) 1794, 1872, 1939
BstKTI GATC 8 cut(s) 369, 654, 1102, 1314, 1426, 2553, 2608, 2637
BstMAI GTCTC 5 cut(s) 192, 366, 574, 1399, 1483
BstMBI GATC 8 cut(s) 366, 651, 1099, 1311, 1423, 2550, 2605, 2634
BstMWI GCNNNNNNNGC 4 cut(s) 155, 1061, 1867, 2401
BstNI CCWGG 3 cut(s) 764, 1097, 2555
BstNSI RCATGY 6 cut(s) 250, 882, 1304, 1369, 1412, 1615
BstSCI CCNGG 4 cut(s) 762, 1095, 1567, 2553
BstSFI CTRYAG 3 cut(s) 1360, 2405, 2442
BstSLI GKGCMC 1 cut(s) 1754
BstV1I GCAGC 6 cut(s) 535, 723, 844, 2202, 2391, 2477
BstV2I GAAGAC 3 cut(s) 432, 1074, 1509
BsuRI GGCC 8 cut(s) 5, 149, 206, 1044, 1567, 2087, 2520, 2684
BtrI CACGTC 1 cut(s) 781
BtsCI GGATG 5 cut(s) 476, 487, 940, 1687, 2625
BtsI GCAGTG 1 cut(s) 2243
BtsIMutI CAGTG 5 cut(s) 82, 1637, 1893, 2243, 2513
BveI ACCTGC 1 cut(s) 1875
Cac8I GCNNGC 4 cut(s) 880, 1018, 1194, 2213
CfoI GCGC 3 cut(s) 1794, 1872, 1939
Cfr13I GGNCC 3 cut(s) 2085, 2158, 2518
CseI GACGC 1 cut(s) 1775
CsiI ACCWGGT 1 cut(s) 762
Csp6I GTAC 5 cut(s) 397, 632, 1685, 2510, 2618
CviQI GTAC 5 cut(s) 397, 632, 1685, 2510, 2618
DdeI CTNAG 6 cut(s) 1175, 1455, 1824, 2613, 2631, 2661
DpnI GATC 8 cut(s) 368, 653, 1101, 1313, 1425, 2552, 2607, 2636
DpnII GATC 8 cut(s) 366, 651, 1099, 1311, 1423, 2550, 2605, 2634
EaeI YGGCCR 1 cut(s) 1565
Eam1104I CTCTTC 8 cut(s) 412, 480, 572, 956, 1596, 2136, 2283, 2358
EarI CTCTTC 8 cut(s) 412, 480, 572, 956, 1596, 2136, 2283, 2358
Ecl136II GAGCTC 1 cut(s) 949
Eco130I CCWWGG 1 cut(s) 2231
Eco147I AGGCCT 1 cut(s) 1044
Eco24I GRGCYC 1 cut(s) 951
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 2158
Eco53kI GAGCTC 1 cut(s) 949
Eco57I CTGAAG 2 cut(s) 1305, 1766
Eco88I CYCGRG 1 cut(s) 2468
EcoICRI GAGCTC 1 cut(s) 949
EcoNI CCTNNNNNAGG 1 cut(s) 929
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 3 cut(s) 762, 1095, 2553
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 2231
EcoT22I ATGCAT 1 cut(s) 1422
EcoT38I GRGCYC 1 cut(s) 951
ErhI CCWWGG 1 cut(s) 2231
FalI AAGNNNNNCTT 4 cut(s) 1151, 1183, 1196, 1228
FaqI GGGAC 5 cut(s) 671, 1078, 1834, 1942, 2467
FauI CCCGC 1 cut(s) 174
FauNDI CATATG 1 cut(s) 2353
FbaI TGATCA 1 cut(s) 651
FblI GTMKAC 2 cut(s) 271, 1448
Fnu4HI GCNGC 6 cut(s) 549, 712, 833, 2216, 2405, 2491
FokI GGATG 5 cut(s) 483, 494, 947, 1694, 2612
FriOI GRGCYC 1 cut(s) 951
Fsp4HI GCNGC 6 cut(s) 549, 712, 833, 2216, 2405, 2491
FspBI CTAG 7 cut(s) 134, 545, 734, 773, 1148, 1670, 2686
FspI TGCGCA 1 cut(s) 1793
GlaI GCGC 3 cut(s) 1793, 1871, 1938
GluI GCNGC 6 cut(s) 549, 712, 833, 2216, 2405, 2491
HaeII RGCGCY 1 cut(s) 1940
HaeIII GGCC 8 cut(s) 5, 149, 206, 1044, 1567, 2087, 2520, 2684
HapII CCGG 2 cut(s) 1568, 2156
HgaI GACGC 1 cut(s) 1775
HhaI GCGC 3 cut(s) 1794, 1872, 1939
Hin1I GRCGYC 1 cut(s) 1786
Hin6I GCGC 3 cut(s) 1792, 1870, 1937
HinP1I GCGC 3 cut(s) 1792, 1870, 1937
HincII GTYRAC 3 cut(s) 272, 1449, 2485
HindII GTYRAC 3 cut(s) 272, 1449, 2485
HindIII AAGCTT 3 cut(s) 126, 646, 2195
HpaII CCGG 2 cut(s) 1568, 2156
HphI GGTGA 3 cut(s) 586, 902, 2440
Hpy166II GTNNAC 9 cut(s) 272, 399, 634, 739, 910, 1449, 1717, 2174, 2485
Hpy8I GTNNAC 9 cut(s) 272, 399, 634, 739, 910, 1449, 1717, 2174, 2485
Hpy99I CGWCG 2 cut(s) 1487, 1521
HpyCH4III ACNGT 3 cut(s) 502, 610, 1154
HpyCH4IV ACGT 3 cut(s) 328, 780, 2223
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 1061, 1867, 2401
HpyF3I CTNAG 6 cut(s) 1175, 1455, 1824, 2613, 2631, 2661
HpySE526I ACGT 3 cut(s) 328, 780, 2223
Hsp92I GRCGYC 1 cut(s) 1786
HspAI GCGC 3 cut(s) 1792, 1870, 1937
Ksp22I TGATCA 1 cut(s) 651
Kzo9I GATC 8 cut(s) 366, 651, 1099, 1311, 1423, 2550, 2605, 2634
LguI GCTCTTC 3 cut(s) 480, 956, 1596
LmnI GCTCC 4 cut(s) 109, 801, 840, 946
Lsp1109I GCAGC 6 cut(s) 535, 723, 844, 2202, 2391, 2477
LweI GCATC 8 cut(s) 130, 201, 1144, 1407, 1419, 1696, 2055, 2448
MabI ACCWGGT 1 cut(s) 762
MaeI CTAG 7 cut(s) 134, 545, 734, 773, 1148, 1670, 2686
MaeII ACGT 3 cut(s) 328, 780, 2223
MaeIII GTNAC 7 cut(s) 13, 345, 729, 853, 1368, 1495, 1833
MalI GATC 8 cut(s) 368, 653, 1101, 1313, 1425, 2552, 2607, 2636
MbiI CCGCTC 1 cut(s) 2665
MboI GATC 8 cut(s) 366, 651, 1099, 1311, 1423, 2550, 2605, 2634
MfeI CAATTG 2 cut(s) 1199, 2559
MhlI GDGCHC 4 cut(s) 951, 984, 1591, 1754
MlyI GAGTC 7 cut(s) 277, 398, 758, 1660, 1784, 1841, 2020
MmeI TCCRAC 1 cut(s) 2674
Mph1103I ATGCAT 1 cut(s) 1422
MroXI GAANNNNTTC 3 cut(s) 128, 1044, 2151
MseI TTAA 8 cut(s) 26, 102, 897, 1413, 1739, 1757, 2094, 2126
MslI CAYNNNNRTG 2 cut(s) 249, 2126
MspA1I CMGCKG 2 cut(s) 1454, 2490
MspI CCGG 2 cut(s) 1568, 2156
MspR9I CCNGG 4 cut(s) 764, 1097, 1569, 2555
MunI CAATTG 2 cut(s) 1199, 2559
Mva1269I GAATGC 1 cut(s) 965
MvaI CCWGG 3 cut(s) 764, 1097, 2555
MwoI GCNNNNNNNGC 4 cut(s) 155, 1061, 1867, 2401
NciI CCSGG 1 cut(s) 1569
NdeI CATATG 1 cut(s) 2353
NdeII GATC 8 cut(s) 366, 651, 1099, 1311, 1423, 2550, 2605, 2634
NmuCI GTSAC 1 cut(s) 1833
NsbI TGCGCA 1 cut(s) 1793
NsiI ATGCAT 1 cut(s) 1422
NspI RCATGY 6 cut(s) 250, 882, 1304, 1369, 1412, 1615
NspV TTCGAA 3 cut(s) 115, 643, 2113
PaeI GCATGC 1 cut(s) 882
PaeR7I CTCGAG 1 cut(s) 2468
PceI AGGCCT 1 cut(s) 1044
PciI ACATGT 3 cut(s) 246, 1408, 1611
PciSI GCTCTTC 3 cut(s) 480, 956, 1596
PcsI WCGNNNNNNNCGW 1 cut(s) 407
PctI GAATGC 1 cut(s) 965
PdmI GAANNNNTTC 3 cut(s) 128, 1044, 2151
PfeI GAWTC 8 cut(s) 388, 846, 1290, 1559, 1650, 1988, 2104, 2587
PflMI CCANNNNNTGG 1 cut(s) 1027
PkrI GCNGC 6 cut(s) 550, 713, 834, 2217, 2406, 2492
PleI GAGTC 7 cut(s) 276, 398, 757, 1660, 1783, 1840, 2020
PpsI GAGTC 7 cut(s) 276, 398, 757, 1660, 1783, 1840, 2020
PscI ACATGT 3 cut(s) 246, 1408, 1611
Psp124BI GAGCTC 1 cut(s) 951
Psp6I CCWGG 3 cut(s) 762, 1095, 2553
PspGI CCWGG 3 cut(s) 762, 1095, 2553
PspPI GGNCC 3 cut(s) 2085, 2158, 2518
PstI CTGCAG 1 cut(s) 2409
PvuII CAGCTG 2 cut(s) 1454, 2490
RsaI GTAC 5 cut(s) 398, 633, 1686, 2511, 2619
RsaNI GTAC 5 cut(s) 397, 632, 1685, 2510, 2618
RseI CAYNNNNRTG 2 cut(s) 249, 2126
SacI GAGCTC 1 cut(s) 951
SalI GTCGAC 2 cut(s) 270, 1447
SapI GCTCTTC 3 cut(s) 480, 956, 1596
SaqAI TTAA 8 cut(s) 26, 102, 897, 1413, 1739, 1757, 2094, 2126
SatI GCNGC 6 cut(s) 549, 712, 833, 2216, 2405, 2491
Sau3AI GATC 8 cut(s) 366, 651, 1099, 1311, 1423, 2550, 2605, 2634
Sau96I GGNCC 3 cut(s) 2085, 2158, 2518
SchI GAGTC 7 cut(s) 277, 398, 758, 1660, 1784, 1841, 2020
ScrFI CCNGG 4 cut(s) 764, 1097, 1569, 2555
SduI GDGCHC 4 cut(s) 951, 984, 1591, 1754
SexAI ACCWGGT 1 cut(s) 762
SfaNI GCATC 8 cut(s) 130, 201, 1144, 1407, 1419, 1696, 2055, 2448
SfcI CTRYAG 3 cut(s) 1360, 2405, 2442
Sfr274I CTCGAG 1 cut(s) 2468
SfuI TTCGAA 3 cut(s) 115, 643, 2113
SinI GGWCC 1 cut(s) 2158
SlaI CTCGAG 1 cut(s) 2468
SmiMI CAYNNNNRTG 2 cut(s) 249, 2126
SmlI CTYRAG 3 cut(s) 50, 1729, 2468
SmoI CTYRAG 3 cut(s) 50, 1729, 2468
SpeI ACTAGT 1 cut(s) 733
SphI GCATGC 1 cut(s) 882
SseBI AGGCCT 1 cut(s) 1044
SsiI CCGC 5 cut(s) 143, 181, 2183, 2377, 2665
SspI AATATT 2 cut(s) 1948, 1973
SspMI CTAG 7 cut(s) 134, 545, 734, 773, 1148, 1670, 2686
SstI GAGCTC 1 cut(s) 951
StuI AGGCCT 1 cut(s) 1044
StyD4I CCNGG 4 cut(s) 762, 1095, 1567, 2553
StyI CCWWGG 1 cut(s) 2231
TaaI ACNGT 3 cut(s) 502, 610, 1154
TaiI ACGT 3 cut(s) 331, 783, 2226
TaqII GACCGA 1 cut(s) 2598
TatI WGTACW 1 cut(s) 1684
TfiI GAWTC 8 cut(s) 388, 846, 1290, 1559, 1650, 1988, 2104, 2587
Tru1I TTAA 8 cut(s) 26, 102, 897, 1413, 1739, 1757, 2094, 2126
Tru9I TTAA 8 cut(s) 26, 102, 897, 1413, 1739, 1757, 2094, 2126
TscAI CASTG 5 cut(s) 82, 1644, 1900, 2250, 2520
TseFI GTSAC 1 cut(s) 1833
TseI GCWGC 6 cut(s) 548, 711, 832, 2215, 2404, 2490
Tsp45I GTSAC 1 cut(s) 1833
TspDTI ATGAA 9 cut(s) 102, 137, 226, 246, 498, 582, 1404, 2154, 2327
TspGWI ACGGA 2 cut(s) 416, 1514
TspRI CASTG 5 cut(s) 82, 1644, 1900, 2250, 2520
Van91I CCANNNNNTGG 1 cut(s) 1027
VpaK11BI GGWCC 1 cut(s) 2158
XagI CCTNNNNNAGG 1 cut(s) 929
XapI RAATTY 6 cut(s) 233, 694, 1916, 1955, 2148, 2544
XbaI TCTAGA 2 cut(s) 772, 1669
XceI RCATGY 6 cut(s) 250, 882, 1304, 1369, 1412, 1615
XcmI CCANNNNNNNNNTGG 3 cut(s) 315, 760, 1093
XhoI CTCGAG 1 cut(s) 2468
XmiI GTMKAC 2 cut(s) 271, 1448
XmnI GAANNNNTTC 3 cut(s) 128, 1044, 2151
XspI CTAG 7 cut(s) 134, 545, 734, 773, 1148, 1670, 2686
Zsp2I ATGCAT 1 cut(s) 1422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.