RchiOBHm_Chr5g0052681

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000110
Physical Location & Seq
Forward (+)
54669956 .. 54673624
3669 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33006

Sequence Viewer

Length: 2709 bp
ATGGCCTTGTCTGTAACGGACCTCTTAATTGGAAAACTTGCGACCATTCTTGAGTATGAAGGAACAACCATAGCAGGTGTTCGTCATGAAGTTGATAAGATTAAGGAGGAGTTTCTAAGCATGAAAGCTTTCCTAGTGGATGCGGAGGCCAACAAACCAAAAACTGAAGGACAGAAATTGTGGGTTGCAAGAATCAGAGACTTAGCCTATGATGTTGAAGATATCATTGATGAATTCATGTATCACATGTATGAGAAGCAAACTGGGGGTCGACTTTCAAGGAGGCTACACAAAACCATTCGCACTCCATGTAATCTTTGGTTCAGGCATAAAATTGCAAAGAAGTTGCGGAAAATTACTGACATGATCAAAGCCATTTCAGAGAGGAATAAAAGATACGGTGTTGGCCTTGTAGGAGGAGCAACTACATCCGAAGATATTCTGAAATTGGTGCAGAGCCAAGCGGAGTCTTCGTTTTTCATTATGGAAGACGAACTGGTTGGGATTGAAGGCAAGAAGCAAATACTAATGGGATGGCTAATGGATGACGAGCAACACCAAACTGTTATATCTGTGGTGGGAATGGGAGGTGCTGGAAAGACAACTCTAGTTGCCAAGACCTTCACCAATGAACTGGTAAAGAGACATTTTGATTGTTATGCATGGATTACTGTTTCCCAAACTTATGATGTGGTACACTTGTTTCGAAGCTTGATCAGGGAACTCTACAAATCAAGGAAGGAAGATTTCCCTGCAAATTTGAATGCCATGAGCCGCATAGAATTGCTAGAATTACTAGTTAACTACTTGGAGTCCAAAAAATACCTAGTGGTTCTAGATGACGTGTGGGATATAAAAATATGGAGAGAAATAAAGGTATCACTTCAAGATAGACAACTTGGAAGTCGAGTCGTACTTACAACTCGAAATGAAGAAGTAGCATCCAACTCTTTTGGAGTTGAAAGCTATGTTCACCGTATTCAGCCCCTGCAAAAGAATGAGGTTTGGGAACTCTTCAGCAGGAAAGCATTCTCAACTAAGCAACACAAAACTTGTCCAACTGAGCTGAAGTCATTAGCTTGCCAACTTGTAGAGAAGTGTGAAGGCCTTCCTCTCGGTATTGTGGCTTTAGGTGGTCTTATGTCTTCTAAGAAGTCGCTGGATCAATGGCAACAAGTCTGCAACAACTTGAATTGGTATCTACATAACAATTCATCGCTAGACCCCGTGAGAAACATTTTGTTGCTGAGTTATAATGATTTGCCATCTCAACTGAAGCATTGCTTCCTCTATTGTTCCCTTTTTCCTGAAGATTATCTATTTAAAAGAAAAAAGCTGACTAGATTGTGGATAGCAGAAGGATTTGTTGAAAATGTGAAAGGGGTCATGCCCGAAGAAGTTGCAGATGGCTATGTTCAGGAACTCTGCTTCCTTAGCATGTTACAGGTTGCAGATGACTTTGTTTCTGCAACATCTTCGGGAAGGCTGAAAAAATTTAAGATGCATGATCTGATGCGAGAGCTTGCTTTGTCAACAGCTGAAAAAGAAAAGTTTGGTTATGTATACGACGGGAGAGAAGTAATGGAGGAGATCTCAACCCGTCGCTTATCAATTCACAGAATGGAAGGAGAAATTAAATCATGGCGGGGTATGTCAAAGATTCGTTCTCTTCTTGTATTTGCCACTGACATGTCCTCATTGTCTTTCTTAAATACACTGGTTTCTCGATTCAAATTGTTGAGGACTCTAGACTTGGAGGATGTCCAAATTGATAATCTGCCGGGTGCAGTTGTTTACTTGTTCAACTTGAGATATTTAAATTTGAAGGGCACTTTAATTAAGGAACTTCCAGAGTCCATAAGGTGTCTCCGCAACCTTCAATTTCTGAACATCAGGGATACTGAGATACAGTCACTTCCCGAAGGAGTTACGAGGTTGCTGAGCTTGCGCAATCTAATTATGTATCGCTACACTGGGAAGCTGTACTTCAAAGTTGTGAGTGGGACAAAAGCGCCATCAAATATTTGTAAACTTATCGGCAACATGACTCAACTTAAGAGCATCGGCATATCAAATGTGAAAGAAAGAGATGAGATTGACCTTTGGCTCTCAATTAAGAAGTTCACTCTGCTTCAACGTCTAGCTTTAACGGCAAGTAATGAAGAGGAAATCCTTCCGGTGAAAGCACAATGTCCGCCTCTTCCGCACCTTCGAAAGCTTATTTTGGTTGGCAGACTGCAAAACGTAATATCTTGGTTTTCTTCACTGCATAGCCTCACAACTCTGTTTCTGCATTGGTCAAGACTTGAAGAGGATTTACTACCTCAAATTGAAGCACTGCTCAATTTGAAAAGGCTTTATCTTTGTAATGCATATGTTGGGGAAGAGTTGTGTTTCCGTAGAGGCTTTGTAAAGCTGGTGAGGTTGGAGTTGTTGAATTTTGCCTCGGTGAAAAAGATAACTATAGAAAATGGGGTGATGCCAAATCTTCGGTACTTATTCGTTGACAGCTTCATGGAGTTGAAGAAAGTGCCATTAGGACTTGAATATCTTTCTACTCTACAATATTTGGGCCTAAAAGATGTTCCCATGGAAGTTGTAAGGCCCATTCAGAAAGGAGGTGTGGATCGCTCTAAGGTACAACACATTTTTGAGATCATACATGTTTTCGAACAGTCATCCAAGTGGGTCCACGAAAACTTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

902

Amino Acids

104.02

Weight (kDa)

8.96

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 93 1.4e-22 Rx N-terminal domain
NB-ARC PF00931 172 - 347 2e-46 NB-ARC domain
WHD_DRP PF23559 434 - 509 1.6e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 553 - 856 4.2e-33 Leucine-rich repeat region
LRR_8 PF13855 580 - 635 2.7e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1254
AarI CACCTGC 1 cut(s) 65
Acc16I TGCGCA 1 cut(s) 1949
Acc36I ACCTGC 1 cut(s) 65
AccI GTMKAC 2 cut(s) 271, 1563
AciI CCGC 8 cut(s) 143, 349, 464, 775, 1645, 1870, 2195, 2204
AclWI GGATC 2 cut(s) 1170, 2634
AcsI RAATTY 5 cut(s) 233, 757, 1493, 1819, 2437
AcuI CTGAAG 5 cut(s) 186, 1000, 1088, 1295, 1329
AfaI GTAC 5 cut(s) 696, 915, 1985, 2495, 2640
AflII CTTAAG 1 cut(s) 2054
AflIII ACRYGT 4 cut(s) 246, 843, 1689, 2662
AhlI ACTAGT 1 cut(s) 796
AjiI CACGTC 1 cut(s) 844
AloI GAACNNNNNNTCC 2 cut(s) 1413, 1445
Alw26I GTCTC 3 cut(s) 192, 637, 1871
AlwI GGATC 2 cut(s) 1170, 2634
AlwNI CAGNNNCTG 1 cut(s) 988
AoxI GGCC 7 cut(s) 3, 147, 406, 1105, 2572, 2603, 2703
ApoI RAATTY 5 cut(s) 233, 757, 1493, 1819, 2437
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2172
AspLEI GCGC 2 cut(s) 1950, 2014
AspS9I GGNCC 4 cut(s) 19, 2572, 2604, 2689
AsuC2I CCSGG 1 cut(s) 1782
AsuHPI GGTGA 6 cut(s) 616, 965, 2191, 2431, 2461, 2488
AsuII TTCGAA 3 cut(s) 706, 2212, 2670
AvaII GGWCC 2 cut(s) 19, 2689
BaeGI GKGCMC 1 cut(s) 1832
BaeI ACNNNNGTAYC 2 cut(s) 686, 719
BarI GAAGNNNNNNTAC 2 cut(s) 2301, 2333
BbsI GAAGAC 3 cut(s) 462, 495, 1137
BccI CCATC 4 cut(s) 528, 1273, 1400, 2023
BceAI ACGGC 1 cut(s) 2166
BcgI CGANNNNNNTGC 4 cut(s) 1382, 1416, 1458, 1492
BciVI GTATCC 1 cut(s) 1891
BclI TGATCA 2 cut(s) 366, 714
BcnI CCSGG 1 cut(s) 1782
BcoDI GTCTC 3 cut(s) 192, 637, 1871
BcuI ACTAGT 1 cut(s) 796
BfmI CTRYAG 1 cut(s) 2463
BfoI RGCGCY 1 cut(s) 2015
BfrI CTTAAG 1 cut(s) 2054
BfuAI ACCTGC 1 cut(s) 65
BfuI GTATCC 1 cut(s) 1891
BglII AGATCT 1 cut(s) 1590
BisI GCNGC 1 cut(s) 775
BlpI GCTNAGC 1 cut(s) 1940
BlsI GCNGC 1 cut(s) 776
Bme1390I CCNGG 1 cut(s) 1782
Bme18I GGWCC 2 cut(s) 19, 2689
BmgBI CACGTC 1 cut(s) 844
BmgT120I GGNCC 4 cut(s) 19, 2572, 2604, 2689
BmiI GGNNCC 1 cut(s) 2690
BmrFI CCNGG 1 cut(s) 1782
BmrI ACTGGG 2 cut(s) 273, 1983
BmsI GCATC 6 cut(s) 130, 950, 1491, 1503, 2070, 2469
BmuI ACTGGG 2 cut(s) 273, 1983
BpiI GAAGAC 3 cut(s) 462, 495, 1137
BplI GAGNNNNNCTC 2 cut(s) 1577, 1609
Bpu10I CCTNAGC 1 cut(s) 1433
Bpu1102I GCTNAGC 1 cut(s) 1940
Bpu14I TTCGAA 3 cut(s) 706, 2212, 2670
BpuEI CTTGAG 2 cut(s) 71, 1828
BpuMI CCSGG 1 cut(s) 1782
BsaJI CCNNGG 2 cut(s) 2445, 2589
BsaWI WCCGGW 1 cut(s) 2176
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bse1I ACTGG 5 cut(s) 268, 501, 639, 1722, 1978
Bse3DI GCAATG 1 cut(s) 1279
BseDI CCNNGG 2 cut(s) 2445, 2589
BseGI GGATG 7 cut(s) 145, 428, 539, 550, 941, 1765, 2678
BseMI GCAATG 1 cut(s) 1279
BseMII CTCAG 4 cut(s) 1053, 1238, 1893, 1931
BseNI ACTGG 5 cut(s) 268, 501, 639, 1722, 1978
BseRI GAGGAG 3 cut(s) 122, 432, 1601
BseSI GKGCMC 1 cut(s) 1832
BsgI GTGCAG 2 cut(s) 473, 1806
BshFI GGCC 7 cut(s) 5, 149, 408, 1107, 2574, 2605, 2705
BsiSI CCGG 2 cut(s) 1781, 2177
BslFI GGGAC 1 cut(s) 2017
BsmAI GTCTC 3 cut(s) 192, 637, 1871
BsmFI GGGAC 1 cut(s) 2017
BsmI GAATGC 2 cut(s) 769, 1028
BsnI GGCC 7 cut(s) 5, 149, 408, 1107, 2574, 2605, 2705
Bsp119I TTCGAA 3 cut(s) 706, 2212, 2670
Bsp1286I GDGCHC 1 cut(s) 1832
Bsp143I GATC 7 cut(s) 366, 714, 1162, 1507, 1590, 2626, 2655
Bsp1720I GCTNAGC 1 cut(s) 1940
Bsp19I CCATGG 1 cut(s) 2589
BspACI CCGC 8 cut(s) 143, 349, 464, 775, 1645, 1870, 2195, 2204
BspANI GGCC 7 cut(s) 5, 149, 408, 1107, 2574, 2605, 2705
BspCNI CTCAG 4 cut(s) 1054, 1239, 1894, 1932
BspHI TCATGA 1 cut(s) 85
BspLI GGNNCC 1 cut(s) 2690
BspMI ACCTGC 1 cut(s) 65
BspPI GGATC 2 cut(s) 1170, 2634
BspT104I TTCGAA 3 cut(s) 706, 2212, 2670
BspTI CTTAAG 1 cut(s) 2054
BsrDI GCAATG 1 cut(s) 1279
BsrI ACTGG 5 cut(s) 268, 501, 639, 1722, 1978
BssECI CCNNGG 2 cut(s) 2445, 2589
BssMI GATC 7 cut(s) 366, 714, 1162, 1507, 1590, 2626, 2655
BssNAI GTATAC 1 cut(s) 1564
BssT1I CCWWGG 1 cut(s) 2589
Bst1107I GTATAC 1 cut(s) 1564
Bst4CI ACNGT 6 cut(s) 401, 565, 673, 977, 1911, 2676
Bst6I CTCTTC 6 cut(s) 1019, 1674, 2157, 2205, 2304, 2379
BstAFI CTTAAG 1 cut(s) 2054
BstBI TTCGAA 3 cut(s) 706, 2212, 2670
BstC8I GCNNGC 3 cut(s) 1081, 1524, 1946
BstDSI CCRYGG 1 cut(s) 2589
BstF5I GGATG 7 cut(s) 145, 428, 539, 550, 941, 1765, 2678
BstH2I RGCGCY 1 cut(s) 2015
BstHHI GCGC 2 cut(s) 1950, 2014
BstKTI GATC 7 cut(s) 369, 717, 1165, 1510, 1593, 2629, 2658
BstMAI GTCTC 3 cut(s) 192, 637, 1871
BstMBI GATC 7 cut(s) 366, 714, 1162, 1507, 1590, 2626, 2655
BstMWI GCNNNNNNNGC 4 cut(s) 1434, 1945, 2150, 2203
BstNSI RCATGY 4 cut(s) 250, 1441, 1693, 2666
BstSCI CCNGG 1 cut(s) 1780
BstSFI CTRYAG 1 cut(s) 2463
BstSLI GKGCMC 1 cut(s) 1832
BstV2I GAAGAC 3 cut(s) 462, 495, 1137
BstX2I RGATCY 1 cut(s) 1590
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 1 cut(s) 1590
BstZ17I GTATAC 1 cut(s) 1564
BsuI GTATCC 1 cut(s) 1891
BsuRI GGCC 7 cut(s) 5, 149, 408, 1107, 2574, 2605, 2705
BtgI CCRYGG 1 cut(s) 2589
BtgZI GCGATG 1 cut(s) 1200
BtrI CACGTC 1 cut(s) 844
BtsCI GGATG 7 cut(s) 145, 428, 539, 550, 941, 1765, 2678
BtsI GCAGTG 2 cut(s) 2264, 2336
BtsIMutI CAGTG 5 cut(s) 1683, 1715, 1971, 2264, 2336
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 3 cut(s) 1081, 1524, 1946
CaiI CAGNNNCTG 1 cut(s) 988
CciI TCATGA 1 cut(s) 85
CfoI GCGC 2 cut(s) 1950, 2014
Cfr13I GGNCC 4 cut(s) 19, 2572, 2604, 2689
Csp6I GTAC 5 cut(s) 695, 914, 1984, 2494, 2639
CviQI GTAC 5 cut(s) 695, 914, 1984, 2494, 2639
DpnI GATC 7 cut(s) 368, 716, 1164, 1509, 1592, 2628, 2657
DpnII GATC 7 cut(s) 366, 714, 1162, 1507, 1590, 2626, 2655
DraI TTTAAA 2 cut(s) 1324, 1818
Eam1104I CTCTTC 6 cut(s) 1019, 1674, 2157, 2205, 2304, 2379
EarI CTCTTC 6 cut(s) 1019, 1674, 2157, 2205, 2304, 2379
EciI GGCGGA 1 cut(s) 2184
Eco130I CCWWGG 1 cut(s) 2589
Eco147I AGGCCT 1 cut(s) 1107
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 2 cut(s) 19, 2689
Eco57I CTGAAG 5 cut(s) 186, 1000, 1088, 1295, 1329
EcoRI GAATTC 1 cut(s) 233
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 2589
EcoT22I ATGCAT 3 cut(s) 664, 1506, 2374
ErhI CCWWGG 1 cut(s) 2589
FalI AAGNNNNNCTT 4 cut(s) 1268, 1300, 1970, 2002
FaqI GGGAC 1 cut(s) 2017
FauI CCCGC 1 cut(s) 1638
FauNDI CATATG 1 cut(s) 2374
FbaI TGATCA 2 cut(s) 366, 714
FblI GTMKAC 2 cut(s) 271, 1563
Fnu4HI GCNGC 1 cut(s) 775
FokI GGATG 7 cut(s) 152, 415, 546, 557, 928, 1772, 2665
Fsp4HI GCNGC 1 cut(s) 775
FspI TGCGCA 1 cut(s) 1949
GlaI GCGC 2 cut(s) 1949, 2013
GluI GCNGC 1 cut(s) 775
HaeII RGCGCY 1 cut(s) 2015
HaeIII GGCC 7 cut(s) 5, 149, 408, 1107, 2574, 2605, 2705
HapII CCGG 2 cut(s) 1781, 2177
HhaI GCGC 2 cut(s) 1950, 2014
Hin6I GCGC 2 cut(s) 1948, 2012
HinP1I GCGC 2 cut(s) 1948, 2012
HincII GTYRAC 4 cut(s) 272, 802, 1533, 2506
HindII GTYRAC 4 cut(s) 272, 802, 1533, 2506
HindIII AAGCTT 3 cut(s) 126, 709, 2216
HinfI GANTC 9 cut(s) 192, 467, 812, 909, 1660, 1728, 1744, 1853, 2047
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 2 cut(s) 1781, 2177
HphI GGTGA 6 cut(s) 616, 965, 2191, 2431, 2461, 2488
Hpy188I TCNGA 7 cut(s) 197, 382, 433, 444, 1512, 1887, 2613
Hpy99I CGWCG 2 cut(s) 1571, 1605
HpyCH4III ACNGT 6 cut(s) 401, 565, 673, 977, 1911, 2676
HpyCH4IV ACGT 3 cut(s) 843, 2137, 2244
HpyF10VI GCNNNNNNNGC 4 cut(s) 1434, 1945, 2150, 2203
HpySE526I ACGT 3 cut(s) 843, 2137, 2244
HspAI GCGC 2 cut(s) 1948, 2012
Ksp22I TGATCA 2 cut(s) 366, 714
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 7 cut(s) 366, 714, 1162, 1507, 1590, 2626, 2655
LmnI GCTCC 1 cut(s) 419
LweI GCATC 6 cut(s) 130, 950, 1491, 1503, 2070, 2469
MaeII ACGT 3 cut(s) 843, 2137, 2244
MaeIII GTNAC 4 cut(s) 13, 1440, 1911, 1927
MalI GATC 7 cut(s) 368, 716, 1164, 1509, 1592, 2628, 2657
MboI GATC 7 cut(s) 366, 714, 1162, 1507, 1590, 2626, 2655
MflI RGATCY 1 cut(s) 1590
MhlI GDGCHC 1 cut(s) 1832
MlyI GAGTC 6 cut(s) 476, 821, 918, 1738, 1862, 2041
MmeI TCCRAC 3 cut(s) 969, 1082, 2406
Mph1103I ATGCAT 3 cut(s) 664, 1506, 2374
MroXI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2172
MslI CAYNNNNRTG 3 cut(s) 249, 1688, 2683
MspA1I CMGCKG 1 cut(s) 1538
MspCI CTTAAG 1 cut(s) 2054
MspI CCGG 2 cut(s) 1781, 2177
MspR9I CCNGG 1 cut(s) 1782
Mva1269I GAATGC 2 cut(s) 769, 1028
MwoI GCNNNNNNNGC 4 cut(s) 1434, 1945, 2150, 2203
NciI CCSGG 1 cut(s) 1782
NcoI CCATGG 1 cut(s) 2589
NdeI CATATG 1 cut(s) 2374
NdeII GATC 7 cut(s) 366, 714, 1162, 1507, 1590, 2626, 2655
NlaIV GGNNCC 1 cut(s) 2690
NmuCI GTSAC 1 cut(s) 1911
NsbI TGCGCA 1 cut(s) 1949
NsiI ATGCAT 3 cut(s) 664, 1506, 2374
NspI RCATGY 4 cut(s) 250, 1441, 1693, 2666
NspV TTCGAA 3 cut(s) 706, 2212, 2670
PacI TTAATTAA 1 cut(s) 1839
PagI TCATGA 1 cut(s) 85
PaqCI CACCTGC 1 cut(s) 65
PceI AGGCCT 1 cut(s) 1107
PciI ACATGT 3 cut(s) 246, 1689, 2662
PctI GAATGC 2 cut(s) 769, 1028
PdmI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2172
PfeI GAWTC 3 cut(s) 192, 1660, 1728
PkrI GCNGC 1 cut(s) 776
PleI GAGTC 6 cut(s) 475, 820, 917, 1738, 1861, 2041
PpsI GAGTC 6 cut(s) 475, 820, 917, 1738, 1861, 2041
PscI ACATGT 3 cut(s) 246, 1689, 2662
PsiI TTATAA 1 cut(s) 1254
PspN4I GGNNCC 1 cut(s) 2690
PspPI GGNCC 4 cut(s) 19, 2572, 2604, 2689
PstNI CAGNNNCTG 1 cut(s) 988
PsuI RGATCY 1 cut(s) 1590
PvuII CAGCTG 1 cut(s) 1538
RsaI GTAC 5 cut(s) 696, 915, 1985, 2495, 2640
RsaNI GTAC 5 cut(s) 695, 914, 1984, 2494, 2639
RseI CAYNNNNRTG 3 cut(s) 249, 1688, 2683
SalI GTCGAC 1 cut(s) 270
SatI GCNGC 1 cut(s) 775
Sau3AI GATC 7 cut(s) 366, 714, 1162, 1507, 1590, 2626, 2655
Sau96I GGNCC 4 cut(s) 19, 2572, 2604, 2689
SchI GAGTC 6 cut(s) 476, 821, 918, 1738, 1862, 2041
ScrFI CCNGG 1 cut(s) 1782
SduI GDGCHC 1 cut(s) 1832
SfaNI GCATC 6 cut(s) 130, 950, 1491, 1503, 2070, 2469
SfcI CTRYAG 1 cut(s) 2463
SfuI TTCGAA 3 cut(s) 706, 2212, 2670
SinI GGWCC 2 cut(s) 19, 2689
SmiI ATTTAAAT 1 cut(s) 1818
SmiMI CAYNNNNRTG 3 cut(s) 249, 1688, 2683
SmlI CTYRAG 3 cut(s) 50, 1807, 2054
SmoI CTYRAG 3 cut(s) 50, 1807, 2054
SpeI ACTAGT 1 cut(s) 796
SseBI AGGCCT 1 cut(s) 1107
SsiI CCGC 8 cut(s) 143, 349, 464, 775, 1645, 1870, 2195, 2204
SspI AATATT 2 cut(s) 2023, 2567
StuI AGGCCT 1 cut(s) 1107
StyD4I CCNGG 1 cut(s) 1780
StyI CCWWGG 1 cut(s) 2589
SwaI ATTTAAAT 1 cut(s) 1818
TaaI ACNGT 6 cut(s) 401, 565, 673, 977, 1911, 2676
TaiI ACGT 3 cut(s) 846, 2140, 2247
TaqI TCGA 7 cut(s) 271, 706, 907, 925, 1726, 2212, 2670
TatI WGTACW 1 cut(s) 1983
TauI GCSGC 1 cut(s) 777
TfiI GAWTC 3 cut(s) 192, 1660, 1728
TscAI CASTG 5 cut(s) 1690, 1722, 1978, 2271, 2343
TseFI GTSAC 1 cut(s) 1911
Tsp45I GTSAC 1 cut(s) 1911
TspGWI ACGGA 2 cut(s) 32, 2387
TspRI CASTG 5 cut(s) 1690, 1722, 1978, 2271, 2343
Vha464I CTTAAG 1 cut(s) 2054
VpaK11BI GGWCC 2 cut(s) 19, 2689
XapI RAATTY 5 cut(s) 233, 757, 1493, 1819, 2437
XbaI TCTAGA 2 cut(s) 835, 1747
XceI RCATGY 4 cut(s) 250, 1441, 1693, 2666
XcmI CCANNNNNNNNNTGG 1 cut(s) 315
XmiI GTMKAC 2 cut(s) 271, 1563
XmnI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2172
Zsp2I ATGCAT 3 cut(s) 664, 1506, 2374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.