Rw5G011860

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000110
Physical Location & Seq
Forward (+)
13692614 .. 13695391
2778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G011860.1

Sequence Viewer

Length: 2778 bp
ATGGCCTCATCTGTAACAGACCTCTTAGCCTCATCTGTAACAGACCTCTTAATTGGAAAACTTGTGACGATTCTTGAGAACGAAGGAACAGCCATAGCTGGTGTTCGTGATGAAGTTGATAAGGTTAAGGAGGAGCTTCAAAGCATGAAAGCATTCCTAGTGGATGCGGAGGCCAACACAGCAAAAACTGAAGGAGAGAAACTGTGGGTTGCAAGAATCAGAGACTTAGCCTATGATGTTGAAGATATCATTGATGAATTCATGTATCACATGTATGAGAAGCAAAGTGGGGGTCGACTTACAAGGGGGCTACACAAAACCATTCGCGCTCCACGCAATCTTTGGTTCAGGCATAAAATTGCAAAGAAGTTGCAGAAAATTACTGAAATGATCAAAGCCATTCCGGAGAGGAATCAAAGATACAGTGTTGGCCTTGTTGGAGGAGCAACTACATCTGAAGATATTCACAAATTGTTGCAGAGCCAAGCAGAGTCTTCGTTTTTCATTATGGAAGACGAACTGGTTGGGATTGAAGGCAAGAAGCAAATACTAATGGGATGGCTCATGGATGAAGAGCAACACCAAACTGTTATATCTGTGGTTGGCATGGGAGGCTCTGGAAAGACAACTCTAGTTGCCAAGACCTTCAACAGTGAAAGGGTGAAGAGACATTTCGATTGTTATGCATGGATTACCGTTTCCCAAACTTATAATGGGGCACACTTGTTTCGAAGCTTGATCAAGGAACTCTACAAATCAAGGAAGGAAGAGTGCTCTGCAAATTTGAATGCCATGAGCCGCACAGAATTGATAGAGTTACTAATTAACTACTTGGAGTCCAACAAATACCTGGTTGTACTAGATGACGTGTGGGATATAAAAGTTTGGAGAGAAATAAAGGTATCACTTCAAGATAGGCAACTCAGAAGTCGAATCATAATTACAACTCGAAAAGAAGAAGTAGCAGCCAATTCTTTTGGAGTTAAAAGCCATGTTCACCGTATTGAACCCCTGCTAAAGAATGAGGCTTGGGAGCTCTTCTGCAAAAAAGCATTCTCATCTAATGAGCACAAAACTTGTCCACCTGATTTTGTGTCATTAACTTGCCAACTTGTTGAAAAGTGTAGAGGCCTTCCTCTGGCTATTGTGGCTTTAGGTGGTCTTATGTCTTCCAAGAATTCGCTGGATCAATGGCAGCAAGTCTGCAACAGCTTGAATTGGCATCTGGATAACAATTCATTGCTGGACCCCGTGAAAAACATCTTGTTGCTGAGTTTCAATGATTTGCCATCTCAATTGAAACATTGCTTCCTCTATTGTTCTCTTTTCCCAGAAGATTTTGTAATTAAGAGAAAAAGGCTCATTAGATTGTGGATAGCCGAAGGATTTGTTGAACATGTGAAAGGGATCACGCTCGAAGAAGTTGCAGATGGTTATCTTTGGGAACTTTGCTTCCGTAGCATGTTACAAGTTGTTGAGAGAAATGTAACAGGAAGGCCGAGAAAAATTAAGATGCATGATCTGATGCGAGAGCTTGGTTTGTCGACAGCCGAGAAGGAAAAGTTTGGTCATGTATACGATGGGAGACAAGTAATGGAAGAGATCTCAACCCGTCGCTTGTCAATTCACAAAATGGAAGGAGAAATTAAATCATGGCCGGGTATGTCAAAGATTCGTTCTCTTCTTGTATTTGCTACTGACATGTCCTCATCGTCTTTCTCAAATGCACTGGTTTCTCGATTCAAATTGTTGAGGACTCTAGACTTGGAGGATGTCGAAATTGATAAACTGCCGGATGCAGTTGTTTATTTCTTCAACTTGAGATATATAAATTTGAGGAACACTTTAATCGAGGAACTTCCAGAGTCCATAGGGCGTCTCCGCAACCTTCAATTTCTGGAACTGAGGCGTACTAAGATAGAGGCACTTCCACAAGGAATTGCAAAATTGCTGAACCTGCGCTATCTAACTATGAGTCGCTTCGCTGGGAACCTTCGAGACTTCAAATTTGTGAGTGGGACAAGAGCGCCATCAAATATTTGTCAGTTGCAGAAATTGCAATGTTTATCAATGATTGAATCAGAAGGGGACACTATTAGACTTATCGGCAACATGACTCAACTCAGGAGCATCGGCATATCGAATGTGAAAGAAAGAGATGAGATTGACCTTTGGGTTTCAATTAAGAAGATGACTCTGCTTCAAACTTTGGTTTTAATGGCAAGTAATGCAGAGGAAATCCTTCCGGTGAATGCACAATTTTCATCTCTTCCGCACCTTAAAAGGCTTCAATTGTTTGGCAGACTGCAAAACGTAATACCTTGGTTTTCTTCACTGCATAGCCTCACATCTCTGTTTCTGCATTGGTCAAGACTTGAAGACGATGTACTACCTCAAATTGAAGCACTGCCCAATCTGACATGGCTTTATCTTAGTAATGCATATGTTGGGGAAGAGTTGTGTTTCCGCAGAGGCTTTGTAAAGCTGGAGACGTTGGAGTTGTTGAATTTTGCCTCGGTGAAAAAGATTACTATAGAGAAAGGGGTGATGCCAAATCTCCGGGAGTTATTGGTTGCACGGTGCATGGAATTGAAGACAATGCCACTAGGCCTTGAATACCTTTCTAATCTACAAACTTTGCAACTGAGGGATGTCCCTACGGAAGTTATAAAGCCCATTCGGAATGGAGGTGCGGATCGGTCTAAGGTACAACACATCCCCGAGATCTTACATTTTTTCGAACAGTCATCCAAGCGGGTCTACGAAAACTTGGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

925

Amino Acids

106.22

Weight (kDa)

8.6

Isoelectric Point (pI)

53.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 16 - 96 2.6e-23 Rx N-terminal domain
NB-ARC PF00931 180 - 355 6.9e-48 NB-ARC domain
WHD_DRP PF23559 442 - 512 2.1e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 557 - 882 4.9e-40 Leucine-rich repeat region
LRR_8 PF13855 584 - 640 1e-05 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 711, 2669
Acc36I ACCTGC 1 cut(s) 1965
AccI GTMKAC 4 cut(s) 295, 1544, 1575, 2760
AccII CGCG 1 cut(s) 327
AccIII TCCGGA 1 cut(s) 403
AciI CCGC 7 cut(s) 167, 799, 1882, 2273, 2467, 2693, 2755
AclWI GGATC 3 cut(s) 1194, 1415, 2703
AcoI YGGCCR 1 cut(s) 1655
AcsI RAATTY 6 cut(s) 257, 781, 1177, 1831, 2006, 2506
AcuI CTGAAG 2 cut(s) 210, 477
AcyI GRCGYC 1 cut(s) 1876
AfaI GTAC 4 cut(s) 858, 1912, 2388, 2709
AfiI CCNNNNNNNGG 1 cut(s) 1138
AflIII ACRYGT 4 cut(s) 270, 867, 1396, 1701
AjiI CACGTC 1 cut(s) 868
AjnI CCWGG 1 cut(s) 849
AloI GAACNNNNNNTCC 2 cut(s) 1437, 1469
AluBI AGCT 7 cut(s) 98, 136, 735, 1036, 1212, 1534, 2485
AluI AGCT 7 cut(s) 98, 136, 735, 1036, 1212, 1534, 2485
Alw21I GWGCWC 3 cut(s) 776, 1038, 1071
Alw26I GTCTC 6 cut(s) 216, 661, 1579, 1883, 1992, 2483
AlwI GGATC 3 cut(s) 1194, 1415, 2703
Ama87I CYCGRG 1 cut(s) 2720
Aor13HI TCCGGA 1 cut(s) 403
AoxI GGCC 7 cut(s) 3, 171, 430, 1129, 1496, 1655, 2608
ApeKI GCWGC 2 cut(s) 965, 1195
ApoI RAATTY 6 cut(s) 257, 781, 1177, 1831, 2006, 2506
ArsI GACNNNNNNTTYG 4 cut(s) 277, 309, 1991, 2023
Asp700I GAANNNNTTC 3 cut(s) 152, 462, 2241
AspLEI GCGC 3 cut(s) 329, 1962, 2029
AspS9I GGNCC 1 cut(s) 1246
AsuC2I CCSGG 2 cut(s) 1659, 2561
AsuHPI GGTGA 5 cut(s) 673, 989, 2260, 2530, 2557
AsuII TTCGAA 2 cut(s) 730, 2739
AvaI CYCGRG 1 cut(s) 2720
AvaII GGWCC 1 cut(s) 1246
BaeGI GKGCMC 1 cut(s) 721
BanII GRGCYC 1 cut(s) 1038
BarI GAAGNNNNNNTAC 2 cut(s) 2370, 2402
BbsI GAAGAC 5 cut(s) 486, 519, 1161, 2385, 2600
Bbv12I GWGCWC 3 cut(s) 776, 1038, 1071
BbvI GCAGC 2 cut(s) 977, 1207
BccI CCATC 5 cut(s) 552, 1297, 1424, 1574, 2038
BcgI CGANNNNNNTGC 6 cut(s) 477, 511, 665, 699, 1406, 1440
BciT130I CCWGG 1 cut(s) 851
BclI TGATCA 2 cut(s) 390, 738
BcnI CCSGG 2 cut(s) 1659, 2561
BcoDI GTCTC 6 cut(s) 216, 661, 1579, 1883, 1992, 2483
BfaI CTAG 5 cut(s) 158, 632, 860, 1760, 2606
BfmI CTRYAG 1 cut(s) 2532
BfoI RGCGCY 1 cut(s) 2030
BfuAI ACCTGC 1 cut(s) 1965
BglII AGATCT 2 cut(s) 1602, 2724
BisI GCNGC 3 cut(s) 799, 966, 1196
BlsI GCNGC 3 cut(s) 800, 967, 1197
Bme1390I CCNGG 3 cut(s) 851, 1659, 2561
Bme18I GGWCC 1 cut(s) 1246
BmeT110I CYCGRG 1 cut(s) 2720
BmgBI CACGTC 1 cut(s) 868
BmgT120I GGNCC 1 cut(s) 1246
BmiI GGNNCC 2 cut(s) 1248, 1991
BmrFI CCNGG 3 cut(s) 851, 1659, 2561
BmsI GCATC 7 cut(s) 154, 1231, 1503, 1515, 1786, 2139, 2538
BpiI GAAGAC 5 cut(s) 486, 519, 1161, 2385, 2600
BpmI CTGGAG 1 cut(s) 2507
Bpu14I TTCGAA 2 cut(s) 730, 2739
BpuEI CTTGAG 2 cut(s) 95, 1840
BpuMI CCSGG 2 cut(s) 1659, 2561
BsaBI GATNNNNATC 1 cut(s) 1434
BsaHI GRCGYC 1 cut(s) 1876
BsaJI CCNNGG 2 cut(s) 2321, 2514
BsaWI WCCGGW 2 cut(s) 403, 2245
Bsc4I CCNNNNNNNGG 1 cut(s) 1138
Bse1I ACTGG 2 cut(s) 525, 1734
Bse3DI GCAATG 3 cut(s) 1238, 1303, 2066
Bse8I GATNNNNATC 1 cut(s) 1434
BseAI TCCGGA 1 cut(s) 403
BseBI CCWGG 1 cut(s) 851
BseDI CCNNGG 2 cut(s) 2321, 2514
BseGI GGATG 8 cut(s) 169, 563, 574, 1777, 1801, 2656, 2715, 2747
BseJI GATNNNNATC 1 cut(s) 1434
BseLI CCNNNNNNNGG 1 cut(s) 1138
BseMI GCAATG 3 cut(s) 1238, 1303, 2066
BseMII CTCAG 5 cut(s) 937, 1262, 1895, 2137, 2636
BseNI ACTGG 2 cut(s) 525, 1734
BseRI GAGGAG 2 cut(s) 146, 456
BseSI GKGCMC 1 cut(s) 721
BseXI GCAGC 2 cut(s) 977, 1207
BseYI CCCAGC 1 cut(s) 1985
Bsh1236I CGCG 1 cut(s) 327
BshFI GGCC 7 cut(s) 5, 173, 432, 1131, 1498, 1657, 2610
BsiHKAI GWGCWC 3 cut(s) 776, 1038, 1071
BsiHKCI CYCGRG 1 cut(s) 2720
BsiSI CCGG 5 cut(s) 404, 1658, 1793, 2246, 2560
BslFI GGGAC 3 cut(s) 2032, 2102, 2639
BslI CCNNNNNNNGG 1 cut(s) 1138
BsmAI GTCTC 6 cut(s) 216, 661, 1579, 1883, 1992, 2483
BsmBI CGTCTC 2 cut(s) 1883, 2483
BsmFI GGGAC 3 cut(s) 2032, 2102, 2639
BsmI GAATGC 4 cut(s) 152, 793, 1052, 2257
BsnI GGCC 7 cut(s) 5, 173, 432, 1131, 1498, 1657, 2610
BsoBI CYCGRG 1 cut(s) 2720
Bsp119I TTCGAA 2 cut(s) 730, 2739
Bsp1286I GDGCHC 4 cut(s) 721, 776, 1038, 1071
Bsp13I TCCGGA 1 cut(s) 403
Bsp143I GATC 8 cut(s) 390, 738, 1186, 1407, 1519, 1602, 2695, 2724
BspACI CCGC 7 cut(s) 167, 799, 1882, 2273, 2467, 2693, 2755
BspANI GGCC 7 cut(s) 5, 173, 432, 1131, 1498, 1657, 2610
BspCNI CTCAG 5 cut(s) 936, 1263, 1896, 2136, 2637
BspEI TCCGGA 1 cut(s) 403
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 2 cut(s) 1248, 1991
BspMI ACCTGC 1 cut(s) 1965
BspPI GGATC 3 cut(s) 1194, 1415, 2703
BspQI GCTCTTC 2 cut(s) 567, 1043
BspT104I TTCGAA 2 cut(s) 730, 2739
BsrDI GCAATG 3 cut(s) 1238, 1303, 2066
BsrI ACTGG 2 cut(s) 525, 1734
BssECI CCNNGG 2 cut(s) 2321, 2514
BssMI GATC 8 cut(s) 390, 738, 1186, 1407, 1519, 1602, 2695, 2724
BssNAI GTATAC 1 cut(s) 1576
BssNI GRCGYC 1 cut(s) 1876
BssT1I CCWWGG 1 cut(s) 2321
Bst1107I GTATAC 1 cut(s) 1576
Bst2UI CCWGG 1 cut(s) 851
Bst4CI ACNGT 8 cut(s) 204, 425, 589, 653, 697, 1001, 2580, 2745
Bst6I CTCTTC 8 cut(s) 567, 659, 762, 1043, 1593, 1686, 2274, 2448
BstACI GRCGYC 1 cut(s) 1876
BstAPI GCANNNNNTGC 2 cut(s) 2056, 2228
BstBI TTCGAA 2 cut(s) 730, 2739
BstF5I GGATG 8 cut(s) 169, 563, 574, 1777, 1801, 2656, 2715, 2747
BstFNI CGCG 1 cut(s) 327
BstH2I RGCGCY 1 cut(s) 2030
BstHHI GCGC 3 cut(s) 329, 1962, 2029
BstKTI GATC 8 cut(s) 393, 741, 1189, 1410, 1522, 1605, 2698, 2727
BstMAI GTCTC 6 cut(s) 216, 661, 1579, 1883, 1992, 2483
BstMBI GATC 8 cut(s) 390, 738, 1186, 1407, 1519, 1602, 2695, 2724
BstMWI GCNNNNNNNGC 8 cut(s) 179, 333, 612, 1148, 1458, 1957, 2056, 2228
BstNI CCWGG 1 cut(s) 851
BstNSI RCATGY 4 cut(s) 274, 1400, 1465, 1705
BstSCI CCNGG 3 cut(s) 849, 1657, 2559
BstSFI CTRYAG 1 cut(s) 2532
BstSLI GKGCMC 1 cut(s) 721
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 2 cut(s) 977, 1207
BstV2I GAAGAC 5 cut(s) 486, 519, 1161, 2385, 2600
BstX2I RGATCY 2 cut(s) 1602, 2724
BstYI RGATCY 2 cut(s) 1602, 2724
BstZ17I GTATAC 1 cut(s) 1576
BsuRI GGCC 7 cut(s) 5, 173, 432, 1131, 1498, 1657, 2610
BtrI CACGTC 1 cut(s) 868
BtsCI GGATG 8 cut(s) 169, 563, 574, 1777, 1801, 2656, 2715, 2747
BtsI GCAGTG 2 cut(s) 2333, 2405
BtsIMutI CAGTG 5 cut(s) 430, 658, 1727, 2333, 2405
BveI ACCTGC 1 cut(s) 1965
CfoI GCGC 3 cut(s) 329, 1962, 2029
Cfr13I GGNCC 1 cut(s) 1246
CseI GACGC 1 cut(s) 1865
CsiI ACCWGGT 1 cut(s) 849
Csp6I GTAC 4 cut(s) 857, 1911, 2387, 2708
CviQI GTAC 4 cut(s) 857, 1911, 2387, 2708
DpnI GATC 8 cut(s) 392, 740, 1188, 1409, 1521, 1604, 2697, 2726
DpnII GATC 8 cut(s) 390, 738, 1186, 1407, 1519, 1602, 2695, 2724
EaeI YGGCCR 1 cut(s) 1655
Eam1104I CTCTTC 8 cut(s) 567, 659, 762, 1043, 1593, 1686, 2274, 2448
EarI CTCTTC 8 cut(s) 567, 659, 762, 1043, 1593, 1686, 2274, 2448
Ecl136II GAGCTC 1 cut(s) 1036
Eco130I CCWWGG 1 cut(s) 2321
Eco147I AGGCCT 2 cut(s) 1131, 2610
Eco24I GRGCYC 1 cut(s) 1038
Eco32I GATATC 1 cut(s) 247
Eco47I GGWCC 1 cut(s) 1246
Eco53kI GAGCTC 1 cut(s) 1036
Eco57I CTGAAG 2 cut(s) 210, 477
Eco88I CYCGRG 1 cut(s) 2720
EcoICRI GAGCTC 1 cut(s) 1036
EcoRI GAATTC 2 cut(s) 257, 1177
EcoRII CCWGG 1 cut(s) 849
EcoRV GATATC 1 cut(s) 247
EcoT14I CCWWGG 1 cut(s) 2321
EcoT22I ATGCAT 3 cut(s) 688, 1518, 2443
EcoT38I GRGCYC 1 cut(s) 1038
ErhI CCWWGG 1 cut(s) 2321
Esp3I CGTCTC 2 cut(s) 1883, 2483
FaqI GGGAC 3 cut(s) 2032, 2102, 2639
FauI CCCGC 1 cut(s) 2748
FauNDI CATATG 1 cut(s) 2443
FbaI TGATCA 2 cut(s) 390, 738
FblI GTMKAC 4 cut(s) 295, 1544, 1575, 2760
Fnu4HI GCNGC 3 cut(s) 799, 966, 1196
FokI GGATG 8 cut(s) 176, 570, 581, 1784, 1808, 2663, 2702, 2734
FriOI GRGCYC 1 cut(s) 1038
Fsp4HI GCNGC 3 cut(s) 799, 966, 1196
FspBI CTAG 5 cut(s) 158, 632, 860, 1760, 2606
GlaI GCGC 3 cut(s) 328, 1961, 2028
GluI GCNGC 3 cut(s) 799, 966, 1196
GsaI CCCAGC 1 cut(s) 1989
GsuI CTGGAG 1 cut(s) 2507
HaeII RGCGCY 1 cut(s) 2030
HaeIII GGCC 7 cut(s) 5, 173, 432, 1131, 1498, 1657, 2610
HapII CCGG 5 cut(s) 404, 1658, 1793, 2246, 2560
HgaI GACGC 1 cut(s) 1865
HhaI GCGC 3 cut(s) 329, 1962, 2029
Hin1I GRCGYC 1 cut(s) 1876
Hin6I GCGC 3 cut(s) 327, 1960, 2027
HinP1I GCGC 3 cut(s) 327, 1960, 2027
HincII GTYRAC 2 cut(s) 296, 1545
HindII GTYRAC 2 cut(s) 296, 1545
HindIII AAGCTT 1 cut(s) 733
HpaII CCGG 5 cut(s) 404, 1658, 1793, 2246, 2560
HphI GGTGA 5 cut(s) 673, 989, 2260, 2530, 2557
Hpy166II GTNNAC 6 cut(s) 296, 997, 1082, 1545, 1576, 2761
Hpy188I TCNGA 7 cut(s) 221, 457, 926, 1524, 2083, 2418, 2682
Hpy8I GTNNAC 6 cut(s) 296, 997, 1082, 1545, 1576, 2761
Hpy99I CGWCG 1 cut(s) 1617
HpyCH4III ACNGT 8 cut(s) 204, 425, 589, 653, 697, 1001, 2580, 2745
HpyCH4IV ACGT 3 cut(s) 867, 2313, 2492
HpyF10VI GCNNNNNNNGC 8 cut(s) 179, 333, 612, 1148, 1458, 1957, 2056, 2228
HpySE526I ACGT 3 cut(s) 867, 2313, 2492
Hsp92I GRCGYC 1 cut(s) 1876
HspAI GCGC 3 cut(s) 327, 1960, 2027
Kpn2I TCCGGA 1 cut(s) 403
Ksp22I TGATCA 2 cut(s) 390, 738
Kzo9I GATC 8 cut(s) 390, 738, 1186, 1407, 1519, 1602, 2695, 2724
LguI GCTCTTC 2 cut(s) 567, 1043
LmnI GCTCC 5 cut(s) 133, 334, 443, 1033, 2127
Lsp1109I GCAGC 2 cut(s) 977, 1207
LweI GCATC 7 cut(s) 154, 1231, 1503, 1515, 1786, 2139, 2538
MabI ACCWGGT 1 cut(s) 849
MaeI CTAG 5 cut(s) 158, 632, 860, 1760, 2606
MaeII ACGT 3 cut(s) 867, 2313, 2492
MaeIII GTNAC 6 cut(s) 13, 37, 64, 816, 1464, 1486
MalI GATC 8 cut(s) 392, 740, 1188, 1409, 1521, 1604, 2697, 2726
MboI GATC 8 cut(s) 390, 738, 1186, 1407, 1519, 1602, 2695, 2724
MfeI CAATTG 2 cut(s) 1295, 2291
MflI RGATCY 2 cut(s) 1602, 2724
MhlI GDGCHC 4 cut(s) 721, 776, 1038, 1071
MlyI GAGTC 7 cut(s) 500, 845, 1750, 1874, 1984, 2110, 2188
MmeI TCCRAC 3 cut(s) 418, 864, 2475
Mph1103I ATGCAT 3 cut(s) 688, 1518, 2443
MroI TCCGGA 1 cut(s) 403
MroXI GAANNNNTTC 3 cut(s) 152, 462, 2241
MslI CAYNNNNRTG 1 cut(s) 273
MspI CCGG 5 cut(s) 404, 1658, 1793, 2246, 2560
MspR9I CCNGG 3 cut(s) 851, 1659, 2561
MunI CAATTG 2 cut(s) 1295, 2291
Mva1269I GAATGC 4 cut(s) 152, 793, 1052, 2257
MvaI CCWGG 1 cut(s) 851
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 8 cut(s) 179, 333, 612, 1148, 1458, 1957, 2056, 2228
NciI CCSGG 2 cut(s) 1659, 2561
NdeI CATATG 1 cut(s) 2443
NdeII GATC 8 cut(s) 390, 738, 1186, 1407, 1519, 1602, 2695, 2724
NlaIV GGNNCC 2 cut(s) 1248, 1991
NmeAIII GCCGAG 2 cut(s) 1524, 1576
NmuCI GTSAC 1 cut(s) 64
NsiI ATGCAT 3 cut(s) 688, 1518, 2443
NspI RCATGY 4 cut(s) 274, 1400, 1465, 1705
NspV TTCGAA 2 cut(s) 730, 2739
PceI AGGCCT 2 cut(s) 1131, 2610
PciI ACATGT 3 cut(s) 270, 1396, 1701
PciSI GCTCTTC 2 cut(s) 567, 1043
PctI GAATGC 4 cut(s) 152, 793, 1052, 2257
PdmI GAANNNNTTC 3 cut(s) 152, 462, 2241
PfeI GAWTC 7 cut(s) 70, 216, 412, 933, 1672, 1740, 2078
PfoI TCCNGGA 1 cut(s) 2559
PkrI GCNGC 3 cut(s) 800, 967, 1197
PleI GAGTC 7 cut(s) 499, 844, 1750, 1873, 1983, 2110, 2188
PpsI GAGTC 7 cut(s) 499, 844, 1750, 1873, 1983, 2110, 2188
PscI ACATGT 3 cut(s) 270, 1396, 1701
PsiI TTATAA 2 cut(s) 711, 2669
Psp124BI GAGCTC 1 cut(s) 1038
Psp6I CCWGG 1 cut(s) 849
PspFI CCCAGC 1 cut(s) 1985
PspGI CCWGG 1 cut(s) 849
PspN4I GGNNCC 2 cut(s) 1248, 1991
PspPI GGNCC 1 cut(s) 1246
PsuI RGATCY 2 cut(s) 1602, 2724
RsaI GTAC 4 cut(s) 858, 1912, 2388, 2709
RsaNI GTAC 4 cut(s) 857, 1911, 2387, 2708
RseI CAYNNNNRTG 1 cut(s) 273
SacI GAGCTC 1 cut(s) 1038
SalI GTCGAC 2 cut(s) 294, 1543
SapI GCTCTTC 2 cut(s) 567, 1043
SatI GCNGC 3 cut(s) 799, 966, 1196
Sau3AI GATC 8 cut(s) 390, 738, 1186, 1407, 1519, 1602, 2695, 2724
Sau96I GGNCC 1 cut(s) 1246
SchI GAGTC 7 cut(s) 500, 845, 1750, 1874, 1984, 2110, 2188
ScrFI CCNGG 3 cut(s) 851, 1659, 2561
SduI GDGCHC 4 cut(s) 721, 776, 1038, 1071
SexAI ACCWGGT 1 cut(s) 849
SfaNI GCATC 7 cut(s) 154, 1231, 1503, 1515, 1786, 2139, 2538
SfcI CTRYAG 1 cut(s) 2532
SfuI TTCGAA 2 cut(s) 730, 2739
SinI GGWCC 1 cut(s) 1246
SmiMI CAYNNNNRTG 1 cut(s) 273
SmlI CTYRAG 2 cut(s) 74, 1819
SmoI CTYRAG 2 cut(s) 74, 1819
SseBI AGGCCT 2 cut(s) 1131, 2610
SsiI CCGC 7 cut(s) 167, 799, 1882, 2273, 2467, 2693, 2755
SspI AATATT 1 cut(s) 2038
SspMI CTAG 5 cut(s) 158, 632, 860, 1760, 2606
SstI GAGCTC 1 cut(s) 1038
StuI AGGCCT 2 cut(s) 1131, 2610
StyD4I CCNGG 3 cut(s) 849, 1657, 2559
StyI CCWWGG 1 cut(s) 2321
TaaI ACNGT 8 cut(s) 204, 425, 589, 653, 697, 1001, 2580, 2745
TaiI ACGT 3 cut(s) 870, 2316, 2495
TaqII GACCGA 1 cut(s) 2688
TatI WGTACW 2 cut(s) 856, 2386
TauI GCSGC 1 cut(s) 801
TfiI GAWTC 7 cut(s) 70, 216, 412, 933, 1672, 1740, 2078
TscAI CASTG 5 cut(s) 430, 658, 1734, 2340, 2412
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 2 cut(s) 965, 1195
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 8 cut(s) 126, 161, 250, 270, 493, 585, 1227, 2253
TspGWI ACGGA 2 cut(s) 1445, 2675
TspRI CASTG 5 cut(s) 430, 658, 1734, 2340, 2412
VpaK11BI GGWCC 1 cut(s) 1246
XapI RAATTY 6 cut(s) 257, 781, 1177, 1831, 2006, 2506
XbaI TCTAGA 1 cut(s) 1759
XceI RCATGY 4 cut(s) 274, 1400, 1465, 1705
XcmI CCANNNNNNNNNTGG 4 cut(s) 339, 710, 847, 1180
XmiI GTMKAC 4 cut(s) 295, 1544, 1575, 2760
XmnI GAANNNNTTC 3 cut(s) 152, 462, 2241
XspI CTAG 5 cut(s) 158, 632, 860, 1760, 2606
Zsp2I ATGCAT 3 cut(s) 688, 1518, 2443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.