MD05G1008300.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000110
Physical Location & Seq
Forward (+)
1833660 .. 1835071
1412 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1008300.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGCAGTTTGAATCAAAGGCAAATATGGCTTTACCCACAGCAGACCTCTTGATTGGAAAAATTGTGACCATTTTTGAGAACGAGGCATCATCCGTAGCCAGTGTTCGTGATGAAATTGATGACATTAAACAGGAGCTTGTGAGCATGAGGGCCTTCCTACATGATGCTGACCGAAAGAAGGCGCAGGCCGAAGGAGAGGCGACGTGGGTTGCAAGCGAAGTCGTGAGGGTCGATTTACAAGGTGGTTCCACCAAACCATATACATTCCGAAGAACGTGGTTTAGACGCAAAATAAGCAGCAAGTTAAAGAACATCTCAAGACTGATTAAAGCCATTCCGGAGAGGAATCAGAGATTTGGTGTTGGTGGATTACAAGGGACTAGTAGTAGCACTTGTGATGATGCTTGCAGATGGGTGCAGAATCGAGCAGAGTCTTCACTTTTTATTATGGAAGATGAGCTCTTAGGGATTGACAGCAAGAAGCAAGAACTGATGGATTGGTTGGTGAATGGAGATCAAGAGCACATGGTTGTCTCTGTAGTCGGGATGGGCGGATCAGGGAAGACAACTCTAGTTGCTAAGGCCTTCAACGATGAAAGAGTGAAGAGACATTTCAATTGTTGTGCGTGGATTACTGTTTCGAAAACATATGTGATTGAAGACTTGTTTAGAAGCTTGATCAAGGAATTCCACCAAGGAAGGATGGAAGAGGTACCTGCAGACATCAATTCCATGACCTATAGAGAATTGCTACAAGTTCTAATCAACTACTTGGAGTCTAAAAGATTTGTAGTTGTGTTGGATGATGTGTGGGATAAAAAACTTTGGGAAGAAATAAAGACATCTCTTCAAAATAGACGGCTTGGAAGTCGAATTATTCTTACTACTCGAAACGAAGACGTTGCATCCTATTCTTATGGACTTGAGTCATTGGCTTGGGATCTTGTAGAGAAGTGTGAAGGCCTACCGCTTGCGCTCGTGGCTTTAGGTGATCTGATGTCTTCCAAGAATTCATCGGAATGGATCAAAGTTTACAACAGCTTAAACTGGCATCTAACTAACCATCCATTACTAGAGCCAGTGGAGAGCATTTTGTTGCTTAGTTTTGATGATTTACCATACCGGTTGAAACATTGTTTTCTATACTGTTCCCTTTTCCCAGAAGATTATTTGATCCGAAGAAAAAGGCTCATTAGGTTGTGGATAGCAGAAGGGTTGTTGAACATGTCAAAGGGATCACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

415

Amino Acids

47.48

Weight (kDa)

6.49

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 17 - 70 4.9e-09 Rx N-terminal domain
NB-ARC PF00931 159 - 307 7.7e-43 NB-ARC domain
AAA_16 PF13191 175 - 270 4.6e-06 AAA ATPase domain
WHD_DRP PF23559 385 - 411 8.6e-07 Disease resistance protein Winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 724
Acc65I GGTACC 1 cut(s) 712
AccB1I GGYRCC 1 cut(s) 712
AccIII TCCGGA 1 cut(s) 337
AciI CCGC 2 cut(s) 552, 968
AclWI GGATC 5 cut(s) 562, 948, 1031, 1168, 1243
AcsI RAATTY 2 cut(s) 686, 1009
AfaI GTAC 1 cut(s) 714
AfiI CCNNNNNNNGG 1 cut(s) 178
AflIII ACRYGT 1 cut(s) 1224
AgeI ACCGGT 1 cut(s) 1122
AgsI TTSAA 7 cut(s) 11, 589, 616, 659, 851, 1129, 1222
AhlI ACTAGT 1 cut(s) 380
AjiI CACGTC 1 cut(s) 204
AluBI AGCT 4 cut(s) 136, 460, 675, 1041
AluI AGCT 4 cut(s) 136, 460, 675, 1041
Alw21I GWGCWC 2 cut(s) 462, 525
Alw26I GTCTC 2 cut(s) 538, 601
AlwI GGATC 5 cut(s) 562, 948, 1031, 1168, 1243
Aor13HI TCCGGA 1 cut(s) 337
AoxI GGCC 4 cut(s) 150, 186, 582, 961
ApeKI GCWGC 1 cut(s) 297
ApoI RAATTY 2 cut(s) 686, 1009
AsiGI ACCGGT 1 cut(s) 1122
Asp718I GGTACC 1 cut(s) 712
AspLEI GCGC 2 cut(s) 184, 976
AspS9I GGNCC 1 cut(s) 150
AsuHPI GGTGA 2 cut(s) 517, 1001
AsuII TTCGAA 1 cut(s) 641
BanI GGYRCC 1 cut(s) 712
BanII GRGCYC 1 cut(s) 462
BauI CACGAG 1 cut(s) 977
BbsI GAAGAC 5 cut(s) 426, 569, 666, 903, 993
Bbv12I GWGCWC 2 cut(s) 462, 525
BbvI GCAGC 1 cut(s) 309
BccI CCATC 5 cut(s) 405, 487, 541, 697, 1071
BceAI ACGGC 1 cut(s) 875
BcgI CGANNNNNNTGC 2 cut(s) 884, 918
BclI TGATCA 1 cut(s) 678
BcoDI GTCTC 2 cut(s) 538, 601
BcuI ACTAGT 1 cut(s) 380
BfaI CTAG 4 cut(s) 381, 572, 1073, 1243
BfmI CTRYAG 3 cut(s) 537, 717, 739
BfuAI ACCTGC 1 cut(s) 724
BisI GCNGC 1 cut(s) 298
BlsI GCNGC 1 cut(s) 299
BmgBI CACGTC 1 cut(s) 204
BmgT120I GGNCC 1 cut(s) 150
BmiI GGNNCC 2 cut(s) 247, 714
BmsI GCATC 5 cut(s) 95, 154, 391, 914, 1060
BoxI GACNNNNGTC 1 cut(s) 925
BpiI GAAGAC 5 cut(s) 426, 569, 666, 903, 993
Bpu10I CCTNAGC 1 cut(s) 579
Bpu14I TTCGAA 1 cut(s) 641
BpuEI CTTGAG 2 cut(s) 301, 944
BsaJI CCNNGG 1 cut(s) 694
BsaWI WCCGGW 2 cut(s) 337, 1122
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse118I RCCGGY 1 cut(s) 1122
Bse1I ACTGG 3 cut(s) 99, 1052, 1079
BseAI TCCGGA 1 cut(s) 337
BseDI CCNNGG 1 cut(s) 694
BseGI GGATG 6 cut(s) 89, 552, 708, 808, 905, 1063
BseLI CCNNNNNNNGG 1 cut(s) 178
BseNI ACTGG 3 cut(s) 99, 1052, 1079
BseXI GCAGC 1 cut(s) 309
BsgI GTGCAG 1 cut(s) 437
BshFI GGCC 4 cut(s) 152, 188, 584, 963
BshNI GGYRCC 1 cut(s) 712
BshTI ACCGGT 1 cut(s) 1122
BsiHKAI GWGCWC 2 cut(s) 462, 525
BsiSI CCGG 2 cut(s) 338, 1123
BslFI GGGAC 1 cut(s) 391
BslI CCNNNNNNNGG 1 cut(s) 178
BsmAI GTCTC 2 cut(s) 538, 601
BsmFI GGGAC 1 cut(s) 391
BsnI GGCC 4 cut(s) 152, 188, 584, 963
Bsp119I TTCGAA 1 cut(s) 641
Bsp1286I GDGCHC 2 cut(s) 462, 525
Bsp13I TCCGGA 1 cut(s) 337
Bsp143I GATC 8 cut(s) 514, 554, 678, 940, 991, 1023, 1173, 1235
BspACI CCGC 2 cut(s) 552, 968
BspANI GGCC 4 cut(s) 152, 188, 584, 963
BspEI TCCGGA 1 cut(s) 337
BspLI GGNNCC 2 cut(s) 247, 714
BspMAI CTGCAG 1 cut(s) 721
BspMI ACCTGC 1 cut(s) 724
BspPI GGATC 5 cut(s) 562, 948, 1031, 1168, 1243
BspT104I TTCGAA 1 cut(s) 641
BspT107I GGYRCC 1 cut(s) 712
BsrFI RCCGGY 1 cut(s) 1122
BsrI ACTGG 3 cut(s) 99, 1052, 1079
BssAI RCCGGY 1 cut(s) 1122
BssECI CCNNGG 1 cut(s) 694
BssMI GATC 8 cut(s) 514, 554, 678, 940, 991, 1023, 1173, 1235
BssSI CACGAG 1 cut(s) 977
BssT1I CCWWGG 1 cut(s) 694
Bst2BI CACGAG 1 cut(s) 977
Bst4CI ACNGT 2 cut(s) 637, 1148
Bst6I CTCTTC 3 cut(s) 599, 702, 852
BstBI TTCGAA 1 cut(s) 641
BstC8I GCNNGC 4 cut(s) 186, 214, 406, 972
BstDEI CTNAG 3 cut(s) 463, 579, 1100
BstF5I GGATG 6 cut(s) 89, 552, 708, 808, 905, 1063
BstHHI GCGC 2 cut(s) 184, 976
BstKTI GATC 8 cut(s) 517, 557, 681, 943, 994, 1026, 1176, 1238
BstMAI GTCTC 2 cut(s) 538, 601
BstMBI GATC 8 cut(s) 514, 554, 678, 940, 991, 1023, 1173, 1235
BstMWI GCNNNNNNNGC 3 cut(s) 26, 294, 980
BstNSI RCATGY 1 cut(s) 1228
BstPAI GACNNNNGTC 1 cut(s) 925
BstSFI CTRYAG 3 cut(s) 537, 717, 739
BstV1I GCAGC 1 cut(s) 309
BstV2I GAAGAC 5 cut(s) 426, 569, 666, 903, 993
BstX2I RGATCY 1 cut(s) 940
BstYI RGATCY 1 cut(s) 940
BsuRI GGCC 4 cut(s) 152, 188, 584, 963
BtrI CACGTC 1 cut(s) 204
BtsCI GGATG 6 cut(s) 89, 552, 708, 808, 905, 1063
BtsIMutI CAGTG 2 cut(s) 106, 1086
BveI ACCTGC 1 cut(s) 724
Cac8I GCNNGC 4 cut(s) 186, 214, 406, 972
CfoI GCGC 2 cut(s) 184, 976
Cfr10I RCCGGY 1 cut(s) 1122
Cfr13I GGNCC 1 cut(s) 150
CseI GACGC 1 cut(s) 294
Csp6I GTAC 1 cut(s) 713
CspAI ACCGGT 1 cut(s) 1122
CviAII CATG 5 cut(s) 145, 161, 526, 733, 1225
CviQI GTAC 1 cut(s) 713
DdeI CTNAG 3 cut(s) 463, 579, 1100
DpnI GATC 8 cut(s) 516, 556, 680, 942, 993, 1025, 1175, 1237
DpnII GATC 8 cut(s) 514, 554, 678, 940, 991, 1023, 1173, 1235
Eam1104I CTCTTC 3 cut(s) 599, 702, 852
EarI CTCTTC 3 cut(s) 599, 702, 852
EciI GGCGGA 1 cut(s) 567
Ecl136II GAGCTC 1 cut(s) 460
Eco130I CCWWGG 1 cut(s) 694
Eco147I AGGCCT 2 cut(s) 584, 963
Eco24I GRGCYC 1 cut(s) 462
Eco53kI GAGCTC 1 cut(s) 460
EcoICRI GAGCTC 1 cut(s) 460
EcoO109I RGGNCCY 1 cut(s) 150
EcoRI GAATTC 2 cut(s) 686, 1009
EcoT14I CCWWGG 1 cut(s) 694
EcoT38I GRGCYC 1 cut(s) 462
ErhI CCWWGG 1 cut(s) 694
FaeI CATG 5 cut(s) 148, 164, 529, 736, 1228
FaqI GGGAC 1 cut(s) 391
FatI CATG 5 cut(s) 144, 160, 525, 732, 1224
FauNDI CATATG 1 cut(s) 649
FbaI TGATCA 1 cut(s) 678
Fnu4HI GCNGC 1 cut(s) 298
FokI GGATG 6 cut(s) 76, 559, 715, 815, 892, 1050
FriOI GRGCYC 1 cut(s) 462
Fsp4HI GCNGC 1 cut(s) 298
FspBI CTAG 4 cut(s) 381, 572, 1073, 1243
GlaI GCGC 2 cut(s) 183, 975
GluI GCNGC 1 cut(s) 298
HaeIII GGCC 4 cut(s) 152, 188, 584, 963
HapII CCGG 2 cut(s) 338, 1123
HgaI GACGC 1 cut(s) 294
HhaI GCGC 2 cut(s) 184, 976
Hin1II CATG 5 cut(s) 148, 164, 529, 736, 1228
Hin6I GCGC 2 cut(s) 182, 974
HinP1I GCGC 2 cut(s) 182, 974
HindIII AAGCTT 1 cut(s) 673
HinfI GANTC 6 cut(s) 11, 346, 421, 431, 776, 926
HpaII CCGG 2 cut(s) 338, 1123
HphI GGTGA 2 cut(s) 517, 1001
Hpy166II GTNNAC 1 cut(s) 1033
Hpy188I TCNGA 5 cut(s) 269, 351, 996, 1018, 1178
Hpy188III TCNNGA 7 cut(s) 49, 107, 223, 318, 338, 518, 544
Hpy8I GTNNAC 1 cut(s) 1033
Hpy99I CGWCG 1 cut(s) 205
HpyAV CCTTC 7 cut(s) 163, 172, 185, 595, 693, 953, 1205
HpyCH4III ACNGT 2 cut(s) 637, 1148
HpyCH4IV ACGT 3 cut(s) 203, 275, 900
HpyCH4V TGCA 6 cut(s) 4, 212, 408, 418, 719, 905
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 294, 980
HpyF3I CTNAG 3 cut(s) 463, 579, 1100
HpySE526I ACGT 3 cut(s) 203, 275, 900
Hsp92II CATG 5 cut(s) 148, 164, 529, 736, 1228
HspAI GCGC 2 cut(s) 182, 974
Kpn2I TCCGGA 1 cut(s) 337
KpnI GGTACC 1 cut(s) 716
Ksp22I TGATCA 1 cut(s) 678
Kzo9I GATC 8 cut(s) 514, 554, 678, 940, 991, 1023, 1173, 1235
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 1 cut(s) 309
LweI GCATC 5 cut(s) 95, 154, 391, 914, 1060
MaeI CTAG 4 cut(s) 381, 572, 1073, 1243
MaeII ACGT 3 cut(s) 203, 275, 900
MaeIII GTNAC 1 cut(s) 64
MalI GATC 8 cut(s) 516, 556, 680, 942, 993, 1025, 1175, 1237
MboI GATC 8 cut(s) 514, 554, 678, 940, 991, 1023, 1173, 1235
MfeI CAATTG 1 cut(s) 616
MflI RGATCY 1 cut(s) 940
MhlI GDGCHC 2 cut(s) 462, 525
MluCI AATT 8 cut(s) 60, 114, 616, 686, 727, 746, 873, 1009
MlyI GAGTC 3 cut(s) 440, 785, 935
MmeI TCCRAC 1 cut(s) 780
MnlI CCTC 7 cut(s) 56, 76, 141, 190, 219, 336, 703
MroI TCCGGA 1 cut(s) 337
MseI TTAA 4 cut(s) 126, 305, 327, 1043
MslI CAYNNNNRTG 1 cut(s) 1018
MspI CCGG 2 cut(s) 338, 1123
MunI CAATTG 1 cut(s) 616
MwoI GCNNNNNNNGC 3 cut(s) 26, 294, 980
NdeI CATATG 1 cut(s) 649
NdeII GATC 8 cut(s) 514, 554, 678, 940, 991, 1023, 1173, 1235
NlaIII CATG 5 cut(s) 148, 164, 529, 736, 1228
NlaIV GGNNCC 2 cut(s) 247, 714
NmuCI GTSAC 1 cut(s) 64
NspI RCATGY 1 cut(s) 1228
NspV TTCGAA 1 cut(s) 641
PceI AGGCCT 2 cut(s) 584, 963
PciI ACATGT 1 cut(s) 1224
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PfeI GAWTC 3 cut(s) 11, 346, 421
PinAI ACCGGT 1 cut(s) 1122
PkrI GCNGC 1 cut(s) 299
PleI GAGTC 3 cut(s) 439, 784, 934
PpsI GAGTC 3 cut(s) 439, 784, 934
PscI ACATGT 1 cut(s) 1224
PshAI GACNNNNGTC 1 cut(s) 925
Psp124BI GAGCTC 1 cut(s) 462
PspN4I GGNNCC 2 cut(s) 247, 714
PspPI GGNCC 1 cut(s) 150
PsrI GAACNNNNNNTAC 2 cut(s) 87, 119
PstI CTGCAG 1 cut(s) 721
PsuI RGATCY 1 cut(s) 940
RsaI GTAC 1 cut(s) 714
RsaNI GTAC 1 cut(s) 713
RseI CAYNNNNRTG 1 cut(s) 1018
SacI GAGCTC 1 cut(s) 462
SaqAI TTAA 4 cut(s) 126, 305, 327, 1043
SatI GCNGC 1 cut(s) 298
Sau3AI GATC 8 cut(s) 514, 554, 678, 940, 991, 1023, 1173, 1235
Sau96I GGNCC 1 cut(s) 150
SchI GAGTC 3 cut(s) 440, 785, 935
SduI GDGCHC 2 cut(s) 462, 525
SfaNI GCATC 5 cut(s) 95, 154, 391, 914, 1060
SfcI CTRYAG 3 cut(s) 537, 717, 739
SfuI TTCGAA 1 cut(s) 641
SmiMI CAYNNNNRTG 1 cut(s) 1018
SmlI CTYRAG 2 cut(s) 316, 923
SmoI CTYRAG 2 cut(s) 316, 923
SpeI ACTAGT 1 cut(s) 380
Sse9I AATT 8 cut(s) 60, 114, 616, 686, 727, 746, 873, 1009
SseBI AGGCCT 2 cut(s) 584, 963
SsiI CCGC 2 cut(s) 552, 968
SspMI CTAG 4 cut(s) 381, 572, 1073, 1243
SstI GAGCTC 1 cut(s) 462
StuI AGGCCT 2 cut(s) 584, 963
StyI CCWWGG 1 cut(s) 694
TaaI ACNGT 2 cut(s) 637, 1148
TaiI ACGT 3 cut(s) 206, 278, 903
TaqI TCGA 5 cut(s) 231, 424, 641, 871, 889
TaqII GACCGA 1 cut(s) 186
TasI AATT 8 cut(s) 60, 114, 616, 686, 727, 746, 873, 1009
TfiI GAWTC 3 cut(s) 11, 346, 421
Tru1I TTAA 4 cut(s) 126, 305, 327, 1043
Tru9I TTAA 4 cut(s) 126, 305, 327, 1043
TscAI CASTG 2 cut(s) 106, 1086
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 1 cut(s) 297
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 3 cut(s) 126, 609, 1002
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 2 cut(s) 106, 1086
XapI RAATTY 2 cut(s) 686, 1009
XceI RCATGY 1 cut(s) 1228
XspI CTAG 4 cut(s) 381, 572, 1073, 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.