Prupe.6G194900_v2.0.a1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000110
Physical Location & Seq
Reverse (-)
20212469 .. 20216840
4372 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G194900.1

Sequence Viewer

Length: 2700 bp
ATGGTGTCAGTTTCAGCAGACCTATTCATTGGCAAATTTGTAGCCATTCTTGAGAGTGAAGCAGCCTCCATAGCGGGTGTTCGTGATCAAGTTGATGTGATTAAGCAGGAGCTGGTATTCATGAAATCCTTCCTAGAGGATGCTGACCGCGGAGAGCAAGCACACACACAAGTACAGAAAGCGTGGGTCGCAAGCTTCCGAGACTTGGCCAATGATGTTGAAAACATCATTGACGAATTCATGTATCACATGTACGAGCAGCAAATCGGCGGTCGATTTGGAAGGTGGATCCGCAGAACCGTTCACTTTCCCAAGAACCTTTGGTATAAGCGTCAAATAGCCAACAAGTTGCAGAAAATCACCGTGGCCATTAGAGCTATTCCAGAGAGAAATCAGAGATATGGTGGTGCTGCAGCTGTAGAAGGAAAAAGTACTTCTGAGGATATTCGAAGATGGGCGCGGAACCAAGCGGAGTCTTCTCTTTATCACAAGGAGGATGAACTTGTCGGGATTGAAGGCGATAAGAACATGTTACTGGGATGGCTGATGGATGAAGTGAAGCACCAAACTGTTGTGTCAGTGGTCGGGATGGGAGGATCAGGGAAGACAACTCTAGTTGCAAGGACCTTCAAAGATGACATTGTAAAGAGACATTTTGAATGCTATGCGTGGATTACTGTTTCCCAGTCTTATGTGATTGAAGACTTACTAAGAAGATTGATCAAAGAATTCCACAAAGCAATGAAGGAAGAGGTCCCTGCAGACATAAATGCCATGAGTTATAACGAATTGCTGGAGATTTTGGTGAACTACTTGGAGACTAAAAGGTACCTAGTTGTATTGGATGACGTGTGGGATGTCCACCTTTGGGACAAAATAAGGTTTTCATTTCCAGATAAACAACTTGGAAGTCGAGTCATGCTTACAACTCGAAGAGAAGACATAGCATCCTCTTCTTTTGGAGTTGAAAGCCATGTTCACAAGATTCAACCGCTGAAAAGGGGTGATGCGTGGGAGCTCTTTAGCATGAAAGCATTCTCAAGTTACCCTAACAAATCTTGTTCACCTGAACTTCTTCCATTAGCTCGGGAACTTGTGGAGAAGTGCCTACCTCTTGCGATCGTAGCCTTGAGCGGTCTCATGTCTTCTAAGAACTCACTTACAGAGTGGAGCACTGTCTACCATAGCTTGAATTGGCATCTCACAAATAATTCTTTGCTAGAACCTATGAAGAGCATCTTGTTGCTTAGTTTCAATGATTTGCCATATAGATTGAAGCAGTGCTTTCTATACTGTTCCCTTTTCCCCGAAGATACAGTGATCACAAATAATAGGCTCATAAGATTGTGGATCGCTGAAGGATTTGTTGAACATGTCAACGGGCTCACGCCAGAAGAAGTTGCAAATAGCTATCTTATGTTACTCATTTTTCGTAACATGCTACAACAAAGGTTTAGAGGACCACTCCCAGCATGTAAGATGCATGATCTTTTGAGGGAGATTGCTCTGTCTATAGCAAAAAAAGAAAAGTTTTGCGCTGTACATGATGGGAGTGAAACAGCAGTAGAAGAAACTGGGGCACCCCGTTTGTCAATTCAAATAACTAATGGAGAAATTGGATCATGCACAGGTATATCACGGCTTCGTTCGTTTCTTGTCTTTGCCACTGGCGTATCCTCATTCTCTTTCTCAAATAAATTATCTTTTGACCTTAAATTGTTGAAGGTTCTAGATTTGGAGGATGTCCCACTTGATTATCTGCCAGACAATCTAATGTCCTTATTTAATTTAAAATATTTAAATTTGAGTGGAACTCCAATTACGGAGCTTCCAGAATCCATTGGAAAGCTCCGCAACCTTCAGACCTTGAATGTCACGCTGACAAAAATAGTGGCACTTCCAAGAGGGATTTCCAAGTTGATAAACTTGCGCCATTTACTTGTGGGCAGGTTCATTTCCCGAAATTTTATTGGGGTAAGAATACCATCAAGTATTAGTAAGATGAAGAAATTACAAACTTTGGCATATATTGAATCAGAAGGAAACATTATTAGACTTATTGGAAGTATGACTCAGCTTACGTTCCTTGGCATTACAAATGTGAAAGAAAGAGACGAGGAGGACCTATGTGCCTCAATTGAAGAGATGAAGGTCCTTTCCCGGTTGTTTTTATCTGTAGCTGATGGAGAGGAATTTCTTCGAGTTAACGCATTGTCTTCACCGCCTCCCTACCTTGATAGACTTGACTTGATCGGCAAACTGGAAAAGGTACCACATTGGTTTTGTTCACTCCACTCTCTCGCATATTTGAATCTGCGTGGGTCCAGACTTGAAGAAGATTTACTACGTCATATTGAAGCATTACCCTCTCTACGTTATCTTTTCCTTGAAGATGCCTCTGTTAGGAAAGAGTTATGTTTCAGCAGAGGCTTTGTGAAGCTTGGGTATTTGCGGTTGCAGAATCTCGCATTATTAAATAAGATAACTATAGAAAAAGGGGCGATGTCAAATCTCGAGTTCTTAGACATTCGTCGTTGCTTGACATTAGAAACATTGCCACAGGGTATTGAGCACCTTACTAAACTACAACGATACATATTTCATAATGTTTCAGAGCAGTTTATGGAGTCCATAGAAGAAGGAGGTGTGGATCATCCAAGGATGCTTCTCGTCGACGAGAGATGTAAGAAATATACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

900

Amino Acids

102.82

Weight (kDa)

7.31

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 783
Acc36I ACCTGC 1 cut(s) 1938
Acc65I GGTACC 2 cut(s) 828, 2269
AccB1I GGYRCC 3 cut(s) 828, 1579, 2269
AccBSI CCGCTC 1 cut(s) 1134
AccI GTMKAC 2 cut(s) 1179, 2673
AccII CGCG 2 cut(s) 150, 460
AclWI GGATC 6 cut(s) 283, 296, 604, 1358, 1627, 2658
AcoI YGGCCR 2 cut(s) 207, 366
AcsI RAATTY 6 cut(s) 35, 236, 728, 1801, 1963, 2192
AcuI CTGAAG 2 cut(s) 1377, 1844
AfaI GTAC 6 cut(s) 174, 254, 433, 830, 1542, 2271
AfiI CCNNNNNNNGG 3 cut(s) 205, 868, 2403
AflIII ACRYGT 4 cut(s) 249, 528, 849, 1372
AjiI CACGTC 1 cut(s) 850
AjuI GAANNNNNNNTTGG 2 cut(s) 1894, 1926
Alw21I GWGCWC 3 cut(s) 1020, 1175, 2574
Alw26I GTCTC 5 cut(s) 195, 643, 812, 1142, 2106
AlwI GGATC 6 cut(s) 283, 296, 604, 1358, 1627, 2658
AlwNI CAGNNNCTG 1 cut(s) 112
Ama87I CYCGRG 2 cut(s) 1086, 2513
AoxI GGCC 2 cut(s) 207, 366
ApeKI GCWGC 4 cut(s) 62, 259, 410, 413
ApoI RAATTY 6 cut(s) 35, 236, 728, 1801, 1963, 2192
ArsI GACNNNNNNTTYG 2 cut(s) 256, 288
Asp700I GAANNNNTTC 4 cut(s) 128, 1034, 1074, 2196
Asp718I GGTACC 2 cut(s) 828, 2269
AspLEI GCGC 3 cut(s) 460, 1538, 1932
AspS9I GGNCC 6 cut(s) 624, 754, 1460, 2122, 2152, 2322
AsuC2I CCSGG 1 cut(s) 2161
AsuHPI GGTGA 5 cut(s) 352, 817, 1016, 1056, 2211
AsuII TTCGAA 1 cut(s) 448
AvaI CYCGRG 2 cut(s) 1086, 2513
AvaII GGWCC 6 cut(s) 624, 754, 1460, 2122, 2152, 2322
BaeGI GKGCMC 1 cut(s) 1582
BaeI ACNNNNGTAYC 2 cut(s) 1656, 1689
BalI TGGCCA 2 cut(s) 209, 368
BamHI GGATCC 1 cut(s) 288
BanI GGYRCC 3 cut(s) 828, 1579, 2269
BanII GRGCYC 2 cut(s) 1020, 1386
BarI GAAGNNNNNNTAC 4 cut(s) 2325, 2328, 2357, 2360
BbsI GAAGAC 6 cut(s) 468, 611, 708, 945, 1137, 2208
Bbv12I GWGCWC 3 cut(s) 1020, 1175, 2574
BbvI GCAGC 4 cut(s) 74, 271, 397, 425
BccI CCATC 7 cut(s) 447, 534, 541, 583, 1541, 1993, 2177
BceAI ACGGC 1 cut(s) 1655
BciVI GTATCC 1 cut(s) 1684
BclI TGATCA 3 cut(s) 85, 720, 1320
BcnI CCSGG 1 cut(s) 2161
BcoDI GTCTC 5 cut(s) 195, 643, 812, 1142, 2106
BfaI CTAG 5 cut(s) 134, 614, 833, 1220, 1730
BfmI CTRYAG 6 cut(s) 411, 417, 759, 1512, 2175, 2487
BfuAI ACCTGC 1 cut(s) 1938
BfuI GTATCC 1 cut(s) 1684
BisI GCNGC 4 cut(s) 63, 260, 411, 414
BlsI GCNGC 4 cut(s) 64, 261, 412, 415
BmcAI AGTACT 1 cut(s) 433
Bme1390I CCNGG 1 cut(s) 2161
Bme18I GGWCC 6 cut(s) 624, 754, 1460, 2122, 2152, 2322
BmeT110I CYCGRG 2 cut(s) 1086, 2513
BmgBI CACGTC 1 cut(s) 850
BmgT120I GGNCC 6 cut(s) 624, 754, 1460, 2122, 2152, 2322
BmiI GGNNCC 7 cut(s) 290, 464, 756, 830, 1581, 2271, 2323
BmrFI CCNGG 1 cut(s) 2161
BmrI ACTGGG 3 cut(s) 545, 679, 1584
BmsI GCATC 8 cut(s) 130, 956, 997, 1207, 1245, 1470, 2383, 2652
BmuI ACTGGG 3 cut(s) 545, 679, 1584
BoxI GACNNNNGTC 1 cut(s) 2529
BpiI GAAGAC 6 cut(s) 468, 611, 708, 945, 1137, 2208
BplI GAGNNNNNCTC 4 cut(s) 1449, 1481, 1798, 1830
BpmI CTGGAG 1 cut(s) 815
Bpu14I TTCGAA 1 cut(s) 448
BpuEI CTTGAG 3 cut(s) 71, 1024, 1150
BpuMI CCSGG 1 cut(s) 2161
BsaAI YACGTR 1 cut(s) 2697
BsaI GGTCTC 1 cut(s) 1142
BsaJI CCNNGG 4 cut(s) 148, 363, 2086, 2657
Bsc4I CCNNNNNNNGG 3 cut(s) 205, 868, 2403
Bse1I ACTGG 5 cut(s) 540, 685, 1579, 1672, 2265
Bse3DI GCAATG 2 cut(s) 747, 2552
BseDI CCNNGG 4 cut(s) 148, 363, 2086, 2657
BseLI CCNNNNNNNGG 3 cut(s) 205, 868, 2403
BseMI GCAATG 2 cut(s) 747, 2552
BseMII CTCAG 2 cut(s) 429, 2087
BseNI ACTGG 5 cut(s) 540, 685, 1579, 1672, 2265
BseRI GAGGAG 1 cut(s) 2132
BseSI GKGCMC 1 cut(s) 1582
BseXI GCAGC 4 cut(s) 74, 271, 397, 425
BseYI CCCAGC 1 cut(s) 1468
Bsh1236I CGCG 2 cut(s) 150, 460
Bsh1285I CGRYCG 2 cut(s) 274, 1122
BshFI GGCC 2 cut(s) 209, 368
BshNI GGYRCC 3 cut(s) 828, 1579, 2269
BsiEI CGRYCG 2 cut(s) 274, 1122
BsiHKAI GWGCWC 3 cut(s) 1020, 1175, 2574
BsiHKCI CYCGRG 2 cut(s) 1086, 2513
BsiSI CCGG 1 cut(s) 2161
BslFI GGGAC 3 cut(s) 740, 884, 1730
BslI CCNNNNNNNGG 3 cut(s) 205, 868, 2403
BsmAI GTCTC 5 cut(s) 195, 643, 812, 1142, 2106
BsmBI CGTCTC 1 cut(s) 2106
BsmFI GGGAC 3 cut(s) 740, 884, 1730
BsmI GAATGC 2 cut(s) 665, 1034
BsnI GGCC 2 cut(s) 209, 368
Bso31I GGTCTC 1 cut(s) 1142
BsoBI CYCGRG 2 cut(s) 1086, 2513
Bsp119I TTCGAA 1 cut(s) 448
Bsp1286I GDGCHC 5 cut(s) 1020, 1175, 1386, 1582, 2574
Bsp1407I TGTACA 1 cut(s) 1540
BspANI GGCC 2 cut(s) 209, 368
BspCNI CTCAG 2 cut(s) 430, 2086
BspFNI CGCG 2 cut(s) 150, 460
BspHI TCATGA 1 cut(s) 120
BspLI GGNNCC 7 cut(s) 290, 464, 756, 830, 1581, 2271, 2323
BspMAI CTGCAG 2 cut(s) 415, 763
BspMI ACCTGC 1 cut(s) 1938
BspPI GGATC 6 cut(s) 283, 296, 604, 1358, 1627, 2658
BspQI GCTCTTC 1 cut(s) 1226
BspT104I TTCGAA 1 cut(s) 448
BspT107I GGYRCC 3 cut(s) 828, 1579, 2269
BspTNI GGTCTC 1 cut(s) 1142
BsrBI CCGCTC 1 cut(s) 1134
BsrDI GCAATG 2 cut(s) 747, 2552
BsrGI TGTACA 1 cut(s) 1540
BsrI ACTGG 5 cut(s) 540, 685, 1579, 1672, 2265
BssECI CCNNGG 4 cut(s) 148, 363, 2086, 2657
BssT1I CCWWGG 2 cut(s) 2086, 2657
Bst4CI ACNGT 7 cut(s) 301, 364, 571, 679, 1177, 1295, 1318
Bst6I CTCTTC 5 cut(s) 744, 928, 958, 1226, 2136
BstAUI TGTACA 1 cut(s) 1540
BstBAI YACGTR 1 cut(s) 2697
BstBI TTCGAA 1 cut(s) 448
BstC8I GCNNGC 2 cut(s) 159, 193
BstDEI CTNAG 6 cut(s) 438, 710, 1149, 1247, 2073, 2521
BstDSI CCRYGG 2 cut(s) 148, 363
BstENI CCTNNNNNAGG 1 cut(s) 2401
BstFNI CGCG 2 cut(s) 150, 460
BstHHI GCGC 3 cut(s) 460, 1538, 1932
BstMAI GTCTC 5 cut(s) 195, 643, 812, 1142, 2106
BstMCI CGRYCG 2 cut(s) 274, 1122
BstMWI GCNNNNNNNGC 4 cut(s) 71, 188, 374, 1124
BstNSI RCATGY 5 cut(s) 253, 532, 1376, 1441, 1476
BstPAI GACNNNNGTC 1 cut(s) 2529
BstSCI CCNGG 1 cut(s) 2159
BstSFI CTRYAG 6 cut(s) 411, 417, 759, 1512, 2175, 2487
BstSLI GKGCMC 1 cut(s) 1582
BstSNI TACGTA 1 cut(s) 2697
BstUI CGCG 2 cut(s) 150, 460
BstV1I GCAGC 4 cut(s) 74, 271, 397, 425
BstV2I GAAGAC 6 cut(s) 468, 611, 708, 945, 1137, 2208
BstX2I RGATCY 1 cut(s) 288
BstYI RGATCY 1 cut(s) 288
BsuI GTATCC 1 cut(s) 1684
BsuRI GGCC 2 cut(s) 209, 368
BtgI CCRYGG 2 cut(s) 148, 363
BtgZI GCGATG 1 cut(s) 2516
BtrI CACGTC 1 cut(s) 850
BtsI GCAGTG 1 cut(s) 1286
BtsIMutI CAGTG 5 cut(s) 585, 1173, 1286, 1323, 1665
BveI ACCTGC 1 cut(s) 1938
Cac8I GCNNGC 2 cut(s) 159, 193
CaiI CAGNNNCTG 1 cut(s) 112
CciI TCATGA 1 cut(s) 120
CfoI GCGC 3 cut(s) 460, 1538, 1932
Cfr13I GGNCC 6 cut(s) 624, 754, 1460, 2122, 2152, 2322
Cfr42I CCGCGG 1 cut(s) 151
CseI GACGC 1 cut(s) 320
Csp6I GTAC 6 cut(s) 173, 253, 432, 829, 1541, 2270
CspCI CAANNNNNGTGG 2 cut(s) 1872, 1907
CviQI GTAC 6 cut(s) 173, 253, 432, 829, 1541, 2270
DdeI CTNAG 6 cut(s) 438, 710, 1149, 1247, 2073, 2521
DraI TTTAAA 2 cut(s) 1791, 1800
EaeI YGGCCR 2 cut(s) 207, 366
Eam1104I CTCTTC 5 cut(s) 744, 928, 958, 1226, 2136
EarI CTCTTC 5 cut(s) 744, 928, 958, 1226, 2136
Ecl136II GAGCTC 1 cut(s) 1018
Eco105I TACGTA 1 cut(s) 2697
Eco130I CCWWGG 2 cut(s) 2086, 2657
Eco24I GRGCYC 2 cut(s) 1020, 1386
Eco31I GGTCTC 1 cut(s) 1142
Eco47I GGWCC 6 cut(s) 624, 754, 1460, 2122, 2152, 2322
Eco53kI GAGCTC 1 cut(s) 1018
Eco57I CTGAAG 2 cut(s) 1377, 1844
Eco88I CYCGRG 2 cut(s) 1086, 2513
EcoICRI GAGCTC 1 cut(s) 1018
EcoNI CCTNNNNNAGG 1 cut(s) 2401
EcoO109I RGGNCCY 4 cut(s) 624, 754, 2122, 2152
EcoRI GAATTC 2 cut(s) 236, 728
EcoT14I CCWWGG 2 cut(s) 2086, 2657
EcoT22I ATGCAT 1 cut(s) 1485
EcoT38I GRGCYC 2 cut(s) 1020, 1386
ErhI CCWWGG 2 cut(s) 2086, 2657
Esp3I CGTCTC 1 cut(s) 2106
FalI AAGNNNNNCTT 4 cut(s) 1223, 1255, 1268, 1300
FaqI GGGAC 3 cut(s) 740, 884, 1730
FauI CCCGC 1 cut(s) 67
FbaI TGATCA 3 cut(s) 85, 720, 1320
FblI GTMKAC 2 cut(s) 1179, 2673
Fnu4HI GCNGC 4 cut(s) 63, 260, 411, 414
FriOI GRGCYC 2 cut(s) 1020, 1386
Fsp4HI GCNGC 4 cut(s) 63, 260, 411, 414
FspBI CTAG 5 cut(s) 134, 614, 833, 1220, 1730
GlaI GCGC 3 cut(s) 459, 1537, 1931
GluI GCNGC 4 cut(s) 63, 260, 411, 414
GsaI CCCAGC 1 cut(s) 1472
GsuI CTGGAG 1 cut(s) 815
HaeIII GGCC 2 cut(s) 209, 368
HapII CCGG 1 cut(s) 2161
HgaI GACGC 1 cut(s) 320
HhaI GCGC 3 cut(s) 460, 1538, 1932
Hin6I GCGC 3 cut(s) 458, 1536, 1930
HinP1I GCGC 3 cut(s) 458, 1536, 1930
HincII GTYRAC 3 cut(s) 1378, 2206, 2674
HindII GTYRAC 3 cut(s) 1378, 2206, 2674
HindIII AAGCTT 2 cut(s) 193, 2438
HinfI GANTC 9 cut(s) 473, 915, 985, 1835, 2033, 2071, 2311, 2461, 2627
HpaI GTTAAC 1 cut(s) 2206
HpaII CCGG 1 cut(s) 2161
HphI GGTGA 5 cut(s) 352, 817, 1016, 1056, 2211
Hpy188I TCNGA 6 cut(s) 200, 396, 439, 1863, 2038, 2614
Hpy99I CGWCG 3 cut(s) 2535, 2675, 2678
HpyCH4III ACNGT 7 cut(s) 301, 364, 571, 679, 1177, 1295, 1318
HpyCH4IV ACGT 5 cut(s) 849, 2081, 2347, 2374, 2696
HpyCH4V TGCA 8 cut(s) 352, 413, 620, 761, 1403, 1483, 1626, 2458
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 188, 374, 1124
HpyF3I CTNAG 6 cut(s) 438, 710, 1149, 1247, 2073, 2521
HpySE526I ACGT 5 cut(s) 849, 2081, 2347, 2374, 2696
HspAI GCGC 3 cut(s) 458, 1536, 1930
KpnI GGTACC 2 cut(s) 832, 2273
Ksp22I TGATCA 3 cut(s) 85, 720, 1320
KspAI GTTAAC 1 cut(s) 2206
KspI CCGCGG 1 cut(s) 151
LguI GCTCTTC 1 cut(s) 1226
LmnI GCTCC 5 cut(s) 109, 1015, 1170, 1825, 1854
Lsp1109I GCAGC 4 cut(s) 74, 271, 397, 425
LweI GCATC 8 cut(s) 130, 956, 997, 1207, 1245, 1470, 2383, 2652
MaeI CTAG 5 cut(s) 134, 614, 833, 1220, 1730
MaeII ACGT 5 cut(s) 849, 2081, 2347, 2374, 2696
MaeIII GTNAC 5 cut(s) 531, 1043, 1419, 1433, 1873
MbiI CCGCTC 1 cut(s) 1134
MfeI CAATTG 1 cut(s) 2136
MflI RGATCY 1 cut(s) 288
MhlI GDGCHC 5 cut(s) 1020, 1175, 1386, 1582, 2574
MlsI TGGCCA 2 cut(s) 209, 368
MluNI TGGCCA 2 cut(s) 209, 368
MlyI GAGTC 4 cut(s) 482, 924, 2065, 2636
Mox20I TGGCCA 2 cut(s) 209, 368
Mph1103I ATGCAT 1 cut(s) 1485
MroXI GAANNNNTTC 4 cut(s) 128, 1034, 1074, 2196
MscI TGGCCA 2 cut(s) 209, 368
MseI TTAA 7 cut(s) 102, 1713, 1785, 1790, 1799, 2205, 2474
Msp20I TGGCCA 2 cut(s) 209, 368
MspA1I CMGCKG 3 cut(s) 150, 416, 994
MspI CCGG 1 cut(s) 2161
MspR9I CCNGG 1 cut(s) 2161
MunI CAATTG 1 cut(s) 2136
Mva1269I GAATGC 2 cut(s) 665, 1034
MvnI CGCG 2 cut(s) 150, 460
MwoI GCNNNNNNNGC 4 cut(s) 71, 188, 374, 1124
NciI CCSGG 1 cut(s) 2161
NlaIV GGNNCC 7 cut(s) 290, 464, 756, 830, 1581, 2271, 2323
NmuCI GTSAC 1 cut(s) 1873
NsiI ATGCAT 1 cut(s) 1485
NspI RCATGY 5 cut(s) 253, 532, 1376, 1441, 1476
NspV TTCGAA 1 cut(s) 448
PaeR7I CTCGAG 1 cut(s) 2513
PagI TCATGA 1 cut(s) 120
PciI ACATGT 3 cut(s) 249, 528, 1372
PciSI GCTCTTC 1 cut(s) 1226
PctI GAATGC 2 cut(s) 665, 1034
PdmI GAANNNNTTC 4 cut(s) 128, 1034, 1074, 2196
PfeI GAWTC 5 cut(s) 985, 1835, 2033, 2311, 2461
PkrI GCNGC 4 cut(s) 64, 261, 412, 415
Ple19I CGATCG 1 cut(s) 1122
PleI GAGTC 4 cut(s) 481, 923, 2065, 2635
PpsI GAGTC 4 cut(s) 481, 923, 2065, 2635
Ppu21I YACGTR 1 cut(s) 2697
PpuMI RGGWCCY 4 cut(s) 624, 754, 2122, 2152
PscI ACATGT 3 cut(s) 249, 528, 1372
PshAI GACNNNNGTC 1 cut(s) 2529
PsiI TTATAA 1 cut(s) 783
Psp124BI GAGCTC 1 cut(s) 1020
Psp5II RGGWCCY 4 cut(s) 624, 754, 2122, 2152
PspFI CCCAGC 1 cut(s) 1468
PspN4I GGNNCC 7 cut(s) 290, 464, 756, 830, 1581, 2271, 2323
PspPI GGNCC 6 cut(s) 624, 754, 1460, 2122, 2152, 2322
PspPPI RGGWCCY 4 cut(s) 624, 754, 2122, 2152
PsrI GAACNNNNNNTAC 2 cut(s) 1804, 1836
PstI CTGCAG 2 cut(s) 415, 763
PstNI CAGNNNCTG 1 cut(s) 112
PsuI RGATCY 1 cut(s) 288
PvuI CGATCG 1 cut(s) 1122
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 6 cut(s) 174, 254, 433, 830, 1542, 2271
RsaNI GTAC 6 cut(s) 173, 253, 432, 829, 1541, 2270
SacI GAGCTC 1 cut(s) 1020
SacII CCGCGG 1 cut(s) 151
SalI GTCGAC 1 cut(s) 2672
SapI GCTCTTC 1 cut(s) 1226
SaqAI TTAA 7 cut(s) 102, 1713, 1785, 1790, 1799, 2205, 2474
SatI GCNGC 4 cut(s) 63, 260, 411, 414
Sau96I GGNCC 6 cut(s) 624, 754, 1460, 2122, 2152, 2322
ScaI AGTACT 1 cut(s) 433
SchI GAGTC 4 cut(s) 482, 924, 2065, 2636
ScrFI CCNGG 1 cut(s) 2161
SduI GDGCHC 5 cut(s) 1020, 1175, 1386, 1582, 2574
SfaNI GCATC 8 cut(s) 130, 956, 997, 1207, 1245, 1470, 2383, 2652
SfcI CTRYAG 6 cut(s) 411, 417, 759, 1512, 2175, 2487
Sfr274I CTCGAG 1 cut(s) 2513
Sfr303I CCGCGG 1 cut(s) 151
SfuI TTCGAA 1 cut(s) 448
SgrBI CCGCGG 1 cut(s) 151
SgrDI CGTCGACG 1 cut(s) 2672
SinI GGWCC 6 cut(s) 624, 754, 1460, 2122, 2152, 2322
SlaI CTCGAG 1 cut(s) 2513
SmiI ATTTAAAT 1 cut(s) 1800
SmlI CTYRAG 4 cut(s) 50, 1039, 1129, 2513
SmoI CTYRAG 4 cut(s) 50, 1039, 1129, 2513
SnaBI TACGTA 1 cut(s) 2697
SspI AATATT 1 cut(s) 1796
SspMI CTAG 5 cut(s) 134, 614, 833, 1220, 1730
SstI GAGCTC 1 cut(s) 1020
StyD4I CCNGG 1 cut(s) 2159
StyI CCWWGG 2 cut(s) 2086, 2657
SwaI ATTTAAAT 1 cut(s) 1800
TaaI ACNGT 7 cut(s) 301, 364, 571, 679, 1177, 1295, 1318
TaiI ACGT 5 cut(s) 852, 2084, 2350, 2377, 2699
TaqI TCGA 7 cut(s) 274, 448, 913, 931, 2200, 2514, 2673
TatI WGTACW 3 cut(s) 172, 431, 1540
TfiI GAWTC 5 cut(s) 985, 1835, 2033, 2311, 2461
Tru1I TTAA 7 cut(s) 102, 1713, 1785, 1790, 1799, 2205, 2474
Tru9I TTAA 7 cut(s) 102, 1713, 1785, 1790, 1799, 2205, 2474
TscAI CASTG 5 cut(s) 585, 1180, 1286, 1323, 1672
TseFI GTSAC 1 cut(s) 1873
TseI GCWGC 4 cut(s) 62, 259, 410, 413
Tsp45I GTSAC 1 cut(s) 1873
TspGWI ACGGA 1 cut(s) 1838
TspRI CASTG 5 cut(s) 585, 1180, 1286, 1323, 1672
VpaK11BI GGWCC 6 cut(s) 624, 754, 1460, 2122, 2152, 2322
XagI CCTNNNNNAGG 1 cut(s) 2401
XapI RAATTY 6 cut(s) 35, 236, 728, 1801, 1963, 2192
XbaI TCTAGA 1 cut(s) 1729
XceI RCATGY 5 cut(s) 253, 532, 1376, 1441, 1476
XhoI CTCGAG 1 cut(s) 2513
XmiI GTMKAC 2 cut(s) 1179, 2673
XmnI GAANNNNTTC 4 cut(s) 128, 1034, 1074, 2196
XspI CTAG 5 cut(s) 134, 614, 833, 1220, 1730
ZrmI AGTACT 1 cut(s) 433
Zsp2I ATGCAT 1 cut(s) 1485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.