Rorug05G0502200

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000110
Physical Location & Seq
Forward (+)
68401590 .. 68407275
5686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0502200.1

Sequence Viewer

Length: 2166 bp
ATGGCCACGTCTCCTCTTCTGTCACTCCTTGTCCTTGTCTCATTTCTGGTCCTAGTCCAACCCTCTCACGGTGGTGACATAGCCATCTACTGGGGGCAAAACGGGAACGAAGGTACCCTAAAACAAACCTGCGCCACCGGAAAATACAAGTACGTGAACATAGCCTTCCTCAACGTCTTCGGCGGCGGCCAAACACCACAAATCAACCTAGCAGGCCACTGCAACCCAGTCTCAAATGGTTGCGCAGCCGTCGGCCCCGAAGTCACATTCTGCCAGAGCCTAGGAGTCAAGGTGTTCCTCTCCCTCGGCGGCGGAACCGGAAACTACTCTCTATCTTCTGCTTCTGACGCAAAAGATGTAGCCGATTATCTATGGAACAGTTTCCTGGGGGGAAAAGGAAACGGCAGAGACAAGTCTACTACTACACGTCCCTTCAGCGATGCCGTTTTGGATGGGATAGATTTCGACTTAAACGTTGAAAATCCATATTGGGATGACCTTGCACGTTATCTCAAGGCGTATAGCAATCCAAGAAGAGCTGTGTACTTAGCTGCAGCTCCTCAGTGTCCATTTCCCGATGCGTTCGTCGGCGGTGCGATTAGTACGGGGCTTTTCGATTATGTTTGGGTTCAGTTCTACGACAATCCTCCATGCCAGTATAGCTCAGGAAACACTGGCAATATTTTGAACTCGTGGAATGAATGGACTACGTCTATACAAGCAGGGAAGATATTCTTGGGGTTGCCGGCGGCTCCAGCGGCTGCTGTAAGCGGGTTCATTCCGGCTACGGTGCTGATTTCTGAGATACTTCCGGTGATAAAGAAGTCGTCGAAGTATGGTGGCGTGATGTTGTGGTCAAAGCATGATGATGATCAAAGTGGTTATAGTTCCTTCATTATCAAAAATGCACTATTAATACCAGCGAATGGTCCAACTTCAGTAAACCCTGCTGCAGCTCCAGGGGGAAGTCCAGGAAAGAAGCAGAGTAATAAAGCTCGGACTATAATCGTTATTGTAGTGGCAATTGTTGTTTCTCTGGTATTGGTTATACTTATATGCATTTGCATTTACGTGAGAATCAGGAAGACGAAGAAAAACCTTGAAGGCTCATCCGAAGTGAGAATTGCAGAAACATATGGCCTGCAAGTCCCCTTTCGGTCTATTAGAGATGCAACGAATGACTTTTCTGAATCAAACAAGCTCGGACGGGGTGGATTTGGTGTTGTTTACAGAGGCAGGCTTTGGGACAAAATAGATGTAGCAGTGAAAAGGCTTTCTAGAGATTCTTCACAAGGAGATATAGAATTCAAAAATGAGGTTGAACTTGTGGCCAAACTTCAGCACCGGAATTTAGTTAGGCTCCTTGGTTTCTGCCTGGAACGAAGCGAAAGAATTCTTATCTATGAGTTTGTTCCAAACGCAAGTCTCGATCATTTCATATTTGATCCAGTCAAGCGCGAACAGTTGGATTGGGATATTCGCTACAAGATCATAATAGGCATTGGGCGGGGACTCCTTTACCTTCATGAAGATTCCCGTCTAAAAATTATCCATCGTGATCTAAAAGCTAGTAATATTCTGTTAGATGCAGAAATGAACCCCAAAATTGCAGATTTCGGATTGGCAAGATTATTTGATACGGATCAAACACAAGGTGAAACTAATCGAATTGTGGGGACCTATGGATATATGGCTCCAGAGTACGTAACGCGTGGACACTTTTCGGTGAAGTCCGATGTTTACAGTTTTGGTGTGCTGGTTTTGGAGATAATAACCGGACAGAAAAATAGTTCTTTTCGGCATGAAGAAAGTGCAGAGGTTCTTCTAAGCTACGCCTGGAATAGTTGGAGGGAGGGGACAGCCTTAAACTTAATAGATCCCATGCTAAGAAGTGGTTTTTCAGGACCTGAAATAATGAGATACATTCACATTGCATTGTTGTGTGTGCAACAAAATTTAGCTGCTAGGCCAACCATGGCTGCAGTTAACCACATGTACAGCAACAACTCTCTCAATCTCCCTGCACCCTCACAACCAGCATTCTTTATGCCTGGGGGCATTGGATCCTCATCCAACACATCGTCGGGAGGGGAGAACAAATCCAGAAACTTGATCCAAATTTCAGCACAGGATATGGATATGATTACGGAGCTATATCCTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

721

Amino Acids

78.97

Weight (kDa)

8.16

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 27 - 251 4.1e-15 Glycosyl hydrolases family 18
Pkinase PF00069 396 - 602 6e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 398 - 660 1e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 244
Acc36I ACCTGC 1 cut(s) 137
Acc65I GGTACC 1 cut(s) 113
AccB1I GGYRCC 1 cut(s) 113
AccB7I CCANNNNNTGG 2 cut(s) 90, 926
AccI GTMKAC 1 cut(s) 416
AccII CGCG 2 cut(s) 1458, 1711
AciI CCGC 9 cut(s) 183, 186, 309, 312, 591, 749, 758, 771, 1507
AclI AACGTT 1 cut(s) 474
AclWI GGATC 6 cut(s) 1439, 1650, 1871, 2058, 2071, 2107
AcoI YGGCCR 3 cut(s) 3, 187, 1329
AcsI RAATTY 5 cut(s) 1304, 1348, 1392, 1954, 2118
AcuI CTGAAG 3 cut(s) 418, 921, 1322
AdeI CACNNNGTG 1 cut(s) 1655
AfaI GTAC 6 cut(s) 115, 152, 545, 604, 1703, 1997
AfiI CCNNNNNNNGG 3 cut(s) 68, 90, 926
AflIII ACRYGT 3 cut(s) 425, 1709, 1992
AgsI TTSAA 5 cut(s) 479, 688, 1103, 1309, 1322
AjiI CACGTC 2 cut(s) 9, 428
AjnI CCWGG 6 cut(s) 384, 958, 970, 1374, 1835, 2050
AjuI GAANNNNNNNTTGG 2 cut(s) 719, 751
AleI CACNNNNGTG 1 cut(s) 72
Alw26I GTCTC 5 cut(s) 15, 43, 235, 402, 1430
AlwI GGATC 6 cut(s) 1439, 1650, 1871, 2058, 2071, 2107
AlwNI CAGNNNCTG 2 cut(s) 761, 1907
AoxI GGCC 7 cut(s) 3, 187, 214, 253, 1138, 1329, 1967
ApeKI GCWGC 8 cut(s) 245, 551, 554, 761, 950, 953, 1961, 1979
ApoI RAATTY 5 cut(s) 1304, 1348, 1392, 1954, 2118
AseI ATTAAT 1 cut(s) 914
Asp700I GAANNNNTTC 3 cut(s) 380, 731, 1392
Asp718I GGTACC 1 cut(s) 113
AspA2I CCTAGG 1 cut(s) 280
AspLEI GCGC 3 cut(s) 134, 245, 1458
AspS9I GGNCC 5 cut(s) 49, 254, 929, 1677, 1904
AsuHPI GGTGA 4 cut(s) 86, 826, 1667, 1738
AvaII GGWCC 4 cut(s) 49, 929, 1677, 1904
AvrII CCTAGG 1 cut(s) 280
BaeI ACNNNNGTAYC 2 cut(s) 97, 130
BalI TGGCCA 2 cut(s) 5, 1331
BamHI GGATCC 1 cut(s) 2063
BanI GGYRCC 1 cut(s) 113
BauI CACGAG 1 cut(s) 691
BbsI GAAGAC 2 cut(s) 169, 1091
BbvI GCAGC 8 cut(s) 257, 538, 566, 748, 937, 965, 1948, 1966
BccI CCATC 3 cut(s) 92, 446, 1560
BceAI ACGGC 3 cut(s) 233, 418, 428
BciT130I CCWGG 6 cut(s) 386, 960, 972, 1376, 1837, 2052
BclI TGATCA 1 cut(s) 871
BcoDI GTCTC 5 cut(s) 15, 43, 235, 402, 1430
BfaI CTAG 7 cut(s) 53, 209, 281, 1278, 1569, 1965, 2164
BfmI CTRYAG 3 cut(s) 552, 951, 1980
BfuAI ACCTGC 1 cut(s) 137
BlnI CCTAGG 1 cut(s) 280
Bme1390I CCNGG 6 cut(s) 386, 960, 972, 1376, 1837, 2052
Bme18I GGWCC 4 cut(s) 49, 929, 1677, 1904
BmgBI CACGTC 2 cut(s) 9, 428
BmgT120I GGNCC 5 cut(s) 49, 254, 929, 1677, 1904
BmiI GGNNCC 8 cut(s) 115, 256, 316, 753, 1361, 1678, 1695, 2065
BmrFI CCNGG 6 cut(s) 386, 960, 972, 1376, 1837, 2052
BmrI ACTGGG 2 cut(s) 100, 221
BmsI GCATC 4 cut(s) 430, 568, 1159, 1576
BmuI ACTGGG 2 cut(s) 100, 221
BpiI GAAGAC 2 cut(s) 169, 1091
BpmI CTGGAG 3 cut(s) 738, 942, 1680
Bpu10I CCTNAGC 1 cut(s) 664
BpuEI CTTGAG 1 cut(s) 497
BsaAI YACGTR 3 cut(s) 154, 1072, 1705
BsaBI GATNNNNATC 3 cut(s) 870, 1641, 2068
BsaJI CCNNGG 7 cut(s) 280, 304, 385, 959, 1363, 1974, 2051
BsaWI WCCGGW 5 cut(s) 137, 317, 811, 1344, 1775
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 90, 926
Bse118I RCCGGY 1 cut(s) 745
Bse1I ACTGG 5 cut(s) 95, 227, 655, 679, 1448
Bse3DI GCAATG 1 cut(s) 1929
Bse8I GATNNNNATC 3 cut(s) 870, 1641, 2068
BseBI CCWGG 6 cut(s) 386, 960, 972, 1376, 1837, 2052
BseDI CCNNGG 7 cut(s) 280, 304, 385, 959, 1363, 1974, 2051
BseGI GGATG 4 cut(s) 457, 499, 1109, 2069
BseJI GATNNNNATC 3 cut(s) 870, 1641, 2068
BseLI CCNNNNNNNGG 3 cut(s) 68, 90, 926
BseMI GCAATG 1 cut(s) 1929
BseMII CTCAG 3 cut(s) 575, 678, 792
BseNI ACTGG 5 cut(s) 95, 227, 655, 679, 1448
BseRI GAGGAG 1 cut(s) 549
BseXI GCAGC 8 cut(s) 257, 538, 566, 748, 937, 965, 1948, 1966
BsgI GTGCAG 2 cut(s) 1833, 2007
Bsh1236I CGCG 2 cut(s) 1458, 1711
BshFI GGCC 7 cut(s) 5, 189, 216, 255, 1140, 1331, 1969
BshNI GGYRCC 1 cut(s) 113
BsiSI CCGG 7 cut(s) 138, 318, 746, 782, 812, 1345, 1776
BslFI GGGAC 6 cut(s) 414, 1133, 1259, 1524, 1690, 1870
BslI CCNNNNNNNGG 3 cut(s) 68, 90, 926
BsmAI GTCTC 5 cut(s) 15, 43, 235, 402, 1430
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 6 cut(s) 414, 1133, 1259, 1524, 1690, 1870
BsmI GAATGC 1 cut(s) 2039
BsnI GGCC 7 cut(s) 5, 189, 216, 255, 1140, 1331, 1969
Bsp1407I TGTACA 1 cut(s) 1995
Bsp143I GATC 9 cut(s) 871, 1429, 1444, 1488, 1558, 1642, 1876, 2063, 2112
Bsp19I CCATGG 1 cut(s) 1974
BspACI CCGC 9 cut(s) 183, 186, 309, 312, 591, 749, 758, 771, 1507
BspANI GGCC 7 cut(s) 5, 189, 216, 255, 1140, 1331, 1969
BspCNI CTCAG 3 cut(s) 574, 677, 793
BspFNI CGCG 2 cut(s) 1458, 1711
BspHI TCATGA 1 cut(s) 1525
BspLI GGNNCC 8 cut(s) 115, 256, 316, 753, 1361, 1678, 1695, 2065
BspMAI CTGCAG 3 cut(s) 556, 955, 1984
BspMI ACCTGC 1 cut(s) 137
BspPI GGATC 6 cut(s) 1439, 1650, 1871, 2058, 2071, 2107
BspQI GCTCTTC 1 cut(s) 529
BspT107I GGYRCC 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 1929
BsrFI RCCGGY 1 cut(s) 745
BsrGI TGTACA 1 cut(s) 1995
BsrI ACTGG 5 cut(s) 95, 227, 655, 679, 1448
BssAI RCCGGY 1 cut(s) 745
BssECI CCNNGG 7 cut(s) 280, 304, 385, 959, 1363, 1974, 2051
BssMI GATC 9 cut(s) 871, 1429, 1444, 1488, 1558, 1642, 1876, 2063, 2112
BssSI CACGAG 1 cut(s) 691
BssT1I CCWWGG 3 cut(s) 280, 1363, 1974
Bst2BI CACGAG 1 cut(s) 691
Bst2UI CCWGG 6 cut(s) 386, 960, 972, 1376, 1837, 2052
Bst4CI ACNGT 5 cut(s) 71, 380, 790, 1464, 1745
Bst6I CTCTTC 2 cut(s) 21, 529
BstAUI TGTACA 1 cut(s) 1995
BstBAI YACGTR 3 cut(s) 154, 1072, 1705
BstC8I GCNNGC 4 cut(s) 214, 747, 1142, 1238
BstDEI CTNAG 6 cut(s) 547, 561, 664, 801, 1826, 1886
BstDSI CCRYGG 1 cut(s) 1974
BstF5I GGATG 4 cut(s) 457, 499, 1109, 2069
BstFNI CGCG 2 cut(s) 1458, 1711
BstHHI GCGC 3 cut(s) 134, 245, 1458
BstKTI GATC 9 cut(s) 874, 1432, 1447, 1491, 1561, 1645, 1879, 2066, 2115
BstMAI GTCTC 5 cut(s) 15, 43, 235, 402, 1430
BstMBI GATC 9 cut(s) 871, 1429, 1444, 1488, 1558, 1642, 1876, 2063, 2112
BstMWI GCNNNNNNNGC 4 cut(s) 347, 660, 755, 758
BstNI CCWGG 6 cut(s) 386, 960, 972, 1376, 1837, 2052
BstNSI RCATGY 1 cut(s) 1996
BstSCI CCNGG 6 cut(s) 384, 958, 970, 1374, 1835, 2050
BstSFI CTRYAG 3 cut(s) 552, 951, 1980
BstSNI TACGTA 1 cut(s) 1705
BstUI CGCG 2 cut(s) 1458, 1711
BstV1I GCAGC 8 cut(s) 257, 538, 566, 748, 937, 965, 1948, 1966
BstV2I GAAGAC 2 cut(s) 169, 1091
BstX2I RGATCY 2 cut(s) 1876, 2063
BstYI RGATCY 2 cut(s) 1876, 2063
BsuRI GGCC 7 cut(s) 5, 189, 216, 255, 1140, 1331, 1969
BtgI CCRYGG 1 cut(s) 1974
BtgZI GCGATG 1 cut(s) 453
BtrI CACGTC 2 cut(s) 9, 428
BtsCI GGATG 4 cut(s) 457, 499, 1109, 2069
BtsI GCAGTG 2 cut(s) 217, 1269
BtsIMutI CAGTG 4 cut(s) 217, 569, 672, 1269
BveI ACCTGC 1 cut(s) 137
Cac8I GCNNGC 4 cut(s) 214, 747, 1142, 1238
CaiI CAGNNNCTG 2 cut(s) 761, 1907
CciI TCATGA 1 cut(s) 1525
CfoI GCGC 3 cut(s) 134, 245, 1458
Cfr10I RCCGGY 1 cut(s) 745
Cfr13I GGNCC 5 cut(s) 49, 254, 929, 1677, 1904
CseI GACGC 1 cut(s) 356
Csp6I GTAC 6 cut(s) 114, 151, 544, 603, 1702, 1996
CviAII CATG 7 cut(s) 651, 863, 1526, 1802, 1882, 1975, 1993
CviQI GTAC 6 cut(s) 114, 151, 544, 603, 1702, 1996
DdeI CTNAG 6 cut(s) 547, 561, 664, 801, 1826, 1886
DpnI GATC 9 cut(s) 873, 1431, 1446, 1490, 1560, 1644, 1878, 2065, 2114
DpnII GATC 9 cut(s) 871, 1429, 1444, 1488, 1558, 1642, 1876, 2063, 2112
DraIII CACNNNGTG 1 cut(s) 1655
EaeI YGGCCR 3 cut(s) 3, 187, 1329
Eam1104I CTCTTC 2 cut(s) 21, 529
EarI CTCTTC 2 cut(s) 21, 529
EciI GGCGGA 1 cut(s) 327
Eco105I TACGTA 1 cut(s) 1705
Eco130I CCWWGG 3 cut(s) 280, 1363, 1974
Eco47I GGWCC 4 cut(s) 49, 929, 1677, 1904
Eco57I CTGAAG 3 cut(s) 418, 921, 1322
EcoO109I RGGNCCY 2 cut(s) 1677, 1904
EcoRI GAATTC 2 cut(s) 1304, 1392
EcoRII CCWGG 6 cut(s) 384, 958, 970, 1374, 1835, 2050
EcoT14I CCWWGG 3 cut(s) 280, 1363, 1974
EcoT22I ATGCAT 1 cut(s) 1061
ErhI CCWWGG 3 cut(s) 280, 1363, 1974
Esp3I CGTCTC 1 cut(s) 15
FaeI CATG 7 cut(s) 654, 866, 1529, 1805, 1885, 1978, 1996
FalI AAGNNNNNCTT 2 cut(s) 719, 751
FaqI GGGAC 6 cut(s) 414, 1133, 1259, 1524, 1690, 1870
FatI CATG 7 cut(s) 650, 862, 1525, 1801, 1881, 1974, 1992
FauI CCCGC 2 cut(s) 764, 1500
FauNDI CATATG 1 cut(s) 1135
FbaI TGATCA 1 cut(s) 871
FblI GTMKAC 1 cut(s) 416
FokI GGATG 4 cut(s) 464, 506, 1096, 2056
FspBI CTAG 7 cut(s) 53, 209, 281, 1278, 1569, 1965, 2164
FspI TGCGCA 1 cut(s) 244
GlaI GCGC 3 cut(s) 133, 244, 1457
GsuI CTGGAG 3 cut(s) 738, 942, 1680
HaeIII GGCC 7 cut(s) 5, 189, 216, 255, 1140, 1331, 1969
HapII CCGG 7 cut(s) 138, 318, 746, 782, 812, 1345, 1776
HgaI GACGC 1 cut(s) 356
HhaI GCGC 3 cut(s) 134, 245, 1458
Hin1II CATG 7 cut(s) 654, 866, 1529, 1805, 1885, 1978, 1996
Hin6I GCGC 3 cut(s) 132, 243, 1456
HinP1I GCGC 3 cut(s) 132, 243, 1456
HincII GTYRAC 1 cut(s) 1987
HindII GTYRAC 1 cut(s) 1987
HinfI GANTC 6 cut(s) 285, 1077, 1190, 1283, 1512, 1532
HpaI GTTAAC 1 cut(s) 1987
HpaII CCGG 7 cut(s) 138, 318, 746, 782, 812, 1345, 1776
HphI GGTGA 4 cut(s) 86, 826, 1667, 1738
Hpy166II GTNNAC 8 cut(s) 157, 417, 544, 943, 1228, 1715, 1741, 1987
Hpy188I TCNGA 8 cut(s) 346, 802, 999, 1114, 1189, 1205, 1619, 1735
Hpy8I GTNNAC 8 cut(s) 157, 417, 544, 943, 1228, 1715, 1741, 1987
Hpy99I CGWCG 4 cut(s) 254, 590, 832, 2086
HpyAV CCTTC 6 cut(s) 104, 175, 442, 901, 1097, 1532
HpyCH4III ACNGT 5 cut(s) 71, 380, 790, 1464, 1745
HpyCH4IV ACGT 9 cut(s) 8, 153, 174, 427, 474, 505, 710, 1071, 1704
HpyF10VI GCNNNNNNNGC 4 cut(s) 347, 660, 755, 758
HpyF3I CTNAG 6 cut(s) 547, 561, 664, 801, 1826, 1886
HpySE526I ACGT 9 cut(s) 8, 153, 174, 427, 474, 505, 710, 1071, 1704
Hsp92II CATG 7 cut(s) 654, 866, 1529, 1805, 1885, 1978, 1996
HspAI GCGC 3 cut(s) 132, 243, 1456
KpnI GGTACC 1 cut(s) 117
KroI GCCGGC 1 cut(s) 745
KroNI GCCGGC 1 cut(s) 747
Ksp22I TGATCA 1 cut(s) 871
KspAI GTTAAC 1 cut(s) 1987
Kzo9I GATC 9 cut(s) 871, 1429, 1444, 1488, 1558, 1642, 1876, 2063, 2112
LguI GCTCTTC 1 cut(s) 529
LmnI GCTCC 6 cut(s) 562, 757, 961, 1365, 1699, 2149
Lsp1109I GCAGC 8 cut(s) 257, 538, 566, 748, 937, 965, 1948, 1966
LweI GCATC 4 cut(s) 430, 568, 1159, 1576
MaeI CTAG 7 cut(s) 53, 209, 281, 1278, 1569, 1965, 2164
MaeII ACGT 9 cut(s) 8, 153, 174, 427, 474, 505, 710, 1071, 1704
MaeIII GTNAC 4 cut(s) 21, 74, 262, 1705
MalI GATC 9 cut(s) 873, 1431, 1446, 1490, 1560, 1644, 1878, 2065, 2114
MboI GATC 9 cut(s) 871, 1429, 1444, 1488, 1558, 1642, 1876, 2063, 2112
MfeI CAATTG 1 cut(s) 1023
MflI RGATCY 2 cut(s) 1876, 2063
MlsI TGGCCA 2 cut(s) 5, 1331
MluI ACGCGT 1 cut(s) 1709
MluNI TGGCCA 2 cut(s) 5, 1331
MlyI GAGTC 2 cut(s) 294, 1506
MmeI TCCRAC 5 cut(s) 82, 956, 1446, 1826, 2097
Mox20I TGGCCA 2 cut(s) 5, 1331
Mph1103I ATGCAT 1 cut(s) 1061
MroNI GCCGGC 1 cut(s) 745
MroXI GAANNNNTTC 3 cut(s) 380, 731, 1392
MscI TGGCCA 2 cut(s) 5, 1331
MseI TTAA 5 cut(s) 470, 914, 1865, 1871, 1986
MslI CAYNNNNRTG 4 cut(s) 72, 867, 1070, 1939
Msp20I TGGCCA 2 cut(s) 5, 1331
MspA1I CMGCKG 1 cut(s) 758
MspI CCGG 7 cut(s) 138, 318, 746, 782, 812, 1345, 1776
MspR9I CCNGG 6 cut(s) 386, 960, 972, 1376, 1837, 2052
MunI CAATTG 1 cut(s) 1023
Mva1269I GAATGC 1 cut(s) 2039
MvaI CCWGG 6 cut(s) 386, 960, 972, 1376, 1837, 2052
MvnI CGCG 2 cut(s) 1458, 1711
MwoI GCNNNNNNNGC 4 cut(s) 347, 660, 755, 758
NaeI GCCGGC 1 cut(s) 747
NcoI CCATGG 1 cut(s) 1974
NdeI CATATG 1 cut(s) 1135
NdeII GATC 9 cut(s) 871, 1429, 1444, 1488, 1558, 1642, 1876, 2063, 2112
NgoMIV GCCGGC 1 cut(s) 745
NlaIII CATG 7 cut(s) 654, 866, 1529, 1805, 1885, 1978, 1996
NlaIV GGNNCC 8 cut(s) 115, 256, 316, 753, 1361, 1678, 1695, 2065
NmeAIII GCCGAG 1 cut(s) 285
NmuCI GTSAC 3 cut(s) 21, 74, 262
NsbI TGCGCA 1 cut(s) 244
NsiI ATGCAT 1 cut(s) 1061
NspI RCATGY 1 cut(s) 1996
OliI CACNNNNGTG 1 cut(s) 72
PagI TCATGA 1 cut(s) 1525
PciI ACATGT 1 cut(s) 1992
PciSI GCTCTTC 1 cut(s) 529
PcsI WCGNNNNNNNCGW 2 cut(s) 471, 1425
PctI GAATGC 1 cut(s) 2039
PdiI GCCGGC 1 cut(s) 747
PdmI GAANNNNTTC 3 cut(s) 380, 731, 1392
PfeI GAWTC 4 cut(s) 1077, 1190, 1283, 1532
PflFI GACNNNGTC 1 cut(s) 709
PflMI CCANNNNNTGG 2 cut(s) 90, 926
PfoI TCCNGGA 1 cut(s) 970
PleI GAGTC 2 cut(s) 293, 1506
PpsI GAGTC 2 cut(s) 293, 1506
Ppu21I YACGTR 3 cut(s) 154, 1072, 1705
PpuMI RGGWCCY 2 cut(s) 1677, 1904
PscI ACATGT 1 cut(s) 1992
PshBI ATTAAT 1 cut(s) 914
Psp1406I AACGTT 1 cut(s) 474
Psp5II RGGWCCY 2 cut(s) 1677, 1904
Psp6I CCWGG 6 cut(s) 384, 958, 970, 1374, 1835, 2050
PspGI CCWGG 6 cut(s) 384, 958, 970, 1374, 1835, 2050
PspN4I GGNNCC 8 cut(s) 115, 256, 316, 753, 1361, 1678, 1695, 2065
PspPI GGNCC 5 cut(s) 49, 254, 929, 1677, 1904
PspPPI RGGWCCY 2 cut(s) 1677, 1904
PstI CTGCAG 3 cut(s) 556, 955, 1984
PstNI CAGNNNCTG 2 cut(s) 761, 1907
PsuI RGATCY 2 cut(s) 1876, 2063
PsyI GACNNNGTC 1 cut(s) 709
RsaI GTAC 6 cut(s) 115, 152, 545, 604, 1703, 1997
RsaNI GTAC 6 cut(s) 114, 151, 544, 603, 1702, 1996
RseI CAYNNNNRTG 4 cut(s) 72, 867, 1070, 1939
SapI GCTCTTC 1 cut(s) 529
SaqAI TTAA 5 cut(s) 470, 914, 1865, 1871, 1986
Sau3AI GATC 9 cut(s) 871, 1429, 1444, 1488, 1558, 1642, 1876, 2063, 2112
Sau96I GGNCC 5 cut(s) 49, 254, 929, 1677, 1904
SchI GAGTC 2 cut(s) 294, 1506
ScrFI CCNGG 6 cut(s) 386, 960, 972, 1376, 1837, 2052
SfaNI GCATC 4 cut(s) 430, 568, 1159, 1576
SfcI CTRYAG 3 cut(s) 552, 951, 1980
SinI GGWCC 4 cut(s) 49, 929, 1677, 1904
SmiMI CAYNNNNRTG 4 cut(s) 72, 867, 1070, 1939
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
SnaBI TACGTA 1 cut(s) 1705
SsiI CCGC 9 cut(s) 183, 186, 309, 312, 591, 749, 758, 771, 1507
SspI AATATT 2 cut(s) 682, 1576
SspMI CTAG 7 cut(s) 53, 209, 281, 1278, 1569, 1965, 2164
StyD4I CCNGG 6 cut(s) 384, 958, 970, 1374, 1835, 2050
StyI CCWWGG 3 cut(s) 280, 1363, 1974
TaaI ACNGT 5 cut(s) 71, 380, 790, 1464, 1745
TaiI ACGT 9 cut(s) 11, 156, 177, 430, 477, 508, 713, 1074, 1707
TaqI TCGA 5 cut(s) 465, 615, 830, 1428, 1666
TaqII GACCGA 1 cut(s) 1146
TatI WGTACW 2 cut(s) 543, 1995
TauI GCSGC 5 cut(s) 186, 189, 312, 752, 761
TfiI GAWTC 4 cut(s) 1077, 1190, 1283, 1532
Tru1I TTAA 5 cut(s) 470, 914, 1865, 1871, 1986
Tru9I TTAA 5 cut(s) 470, 914, 1865, 1871, 1986
TscAI CASTG 4 cut(s) 224, 569, 679, 1269
TseFI GTSAC 3 cut(s) 21, 74, 262
TseI GCWGC 8 cut(s) 245, 551, 554, 761, 950, 953, 1961, 1979
Tsp45I GTSAC 3 cut(s) 21, 74, 262
TspDTI ATGAA 8 cut(s) 714, 766, 883, 1426, 1514, 1542, 1610, 1818
TspGWI ACGGA 2 cut(s) 1655, 2162
TspRI CASTG 4 cut(s) 224, 569, 679, 1269
Tth111I GACNNNGTC 1 cut(s) 709
Van91I CCANNNNNTGG 2 cut(s) 90, 926
VpaK11BI GGWCC 4 cut(s) 49, 929, 1677, 1904
VspI ATTAAT 1 cut(s) 914
XapI RAATTY 5 cut(s) 1304, 1348, 1392, 1954, 2118
XbaI TCTAGA 1 cut(s) 1277
XceI RCATGY 1 cut(s) 1996
XmaJI CCTAGG 1 cut(s) 280
XmiI GTMKAC 1 cut(s) 416
XmnI GAANNNNTTC 3 cut(s) 380, 731, 1392
XspI CTAG 7 cut(s) 53, 209, 281, 1278, 1569, 1965, 2164
Zsp2I ATGCAT 1 cut(s) 1061
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.