RchiOBHm_Chr5g0052641

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000110
Physical Location & Seq
Forward (+)
54626172 .. 54628934
2763 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33003

Sequence Viewer

Length: 2763 bp
ATGGCCTTGTCTGTAACGGACCTCTTAATTGGAAAACTTGCGACAATTCTTGAGAACGAAGGAACAACTATAGCAGGTGTTCGTCATGAAGTTGATAAGATTAAGGAGGAGTTTCTAAGCATGAAAGCTTTCCTACTGGATGCGGAGGCCAACAAACCAAAAACTGAAGGACAGAAATTGTGGGTTGCAAGAATCAGAGACTTGGCCTATGATGTTGAAGATATCATTGATGAATTCATGTATCACATGTATGAGAAGCAAAGTGGGGGTCGACTTTCAAGGGGGCTACACAATTCCATTCGCGCTCCATGCAACCTTTGGTTCAGGCATAAAATTGCAAAGAAGTTGCGGAAAATTACTGACATGATCAAAGCCATTCCAGAGAGGAATCAAAGATACGGTGTTGGCCTTGTAGGAGGAGCAACTACATCCGAAGATATTCAGAAATTGGTGCAGAACCAAGCGGAGTCTTCGTTTTACATTATGGAAGACGAACTGGTTGGGATTGAAGGCAAGAAGCAAATACTAATGGGATGGCTAATGGATGATGAGCAACACCAAACTGTTATATCTGTGGTGGGCATGGGAGGTTCTGGAAAGACAACTCTAGTTGCCAAGACCTTCACCAATGAATTGGTAAAGAGACATTTTGATTGTTATGCATGGATTACTGTTTCCCAAACTTATGATGTGGTACACTTGTTTAGAAGCTTGATCAGGGAACTCTACAAATCAAGGAAGGAAAATTTCCCTGCAATTCTGAATGCCATGAGCCGCATAGAATTGCTAGAGTTACTAGTTAACTACTTGGAGTCCAAAAAATACCTAGTTGTTCTAGATGACGTGTGGGATATAAAAGTATGGAGAGAAATAAAGGTATCACTTCAAGATAGACAACTTGGAAGTCGAGTCGTACTTACAACTCGAAATGAAGAAGTAGCATCCAACTCTTTTGGAGTTGAAATCTATATTTACCATATTCAGCCCCTGCAAAAGAAGGAGGCTTGGGAACTCTTCAACAGGAAAGCATTCTCAACTAAGCAACACAAAACTTGTCCAACTGAGCTGAAGTCTTTAGCTTGCCAACTTGTGGAGAGGTGTGAAGGCCTTCCTCTCGGTATTGTGGCTTTAGCTGGTCTTATGTCATCTAAGAAGTCGCTGGATCAATGGCAACAAGTCTGCAACAACTTGAATTGGTATCTACATAACAATTCATCTCTAGACACAATGAGAAACATTTTGTTGTTGAGTTTCACTGATTTGCCATCTCAACTGAAGCATTGCTTCCTCTATTGTTCCCTTTTTCCAGAAGATTATCTATTTAATACAAAAAGGCTGACTAGATTGTGGATAGCAGAAGGATTTGTTGAAAATGTGAAAGGGGTCACTCCTGAAGAAGTTGCAGGTGCCTATCTTCGGGAACTCTGCTTCCGTAGCATGTTACAATTTGCAGATGGCTTTGTTTCCGGATCACCTTTGGGAAGGCTGAGAAAACTTAAGATGCATGATCTGATGCGAGAGCTTGCTTTGTCGACTGCCGAAAAAGAAAAGTTTGGTTATGTATACGACGGGAGAGAAGTAATGGAAGAGATCTCAACCCGTCGCTTATCAATTCATAGAATGAAAGGAGAAATCAAATCATGGAGGGGTATGTCAAAGATTCGTTCTCTTCTTGTATTTGACACTGACATGTTCTCATTGTCTTTCTTAAATACACTGGTTTCTCGATTCAAATTGTTGAGGACTCTAGACTTGGAGGATGTCCAAATTGATAAACTGCCGGCTGCAATTGTTTACATGTTCAACTTGAGATATTTAAATTTGAAGGGCACTTTAATCGAGGAACTTCCGGAGTCCATAAGGCGTCTCCGCAACCTTCAATTTCTGAACATCAGAGATACTAAGATAGAGTCACTTCCACAAGGAATTAAGAGGTTGCTGAACCTGCGGAATCTAATTATGTATCGCTACACTAGGGACCTGTACTTCAAAGCTGCTAGTGGGACAGAAGCGCCATCAAATATTTGTAAGTTGCAGAAATTGCAAAATTTATCAATAATTGAATCAGAAGGGGACACTTGCAGACTTATCGGCAACATGACTCAACTTAAGTACATCGGCATATCCAATGTAAAAGAAAGAGATGAGATTGACCTTTGGGTCTCAATTAAGAAGTTCACTCTGCTTCAATCTCTAGGTTTACTGGCAAGTAATGAAGAGGAAATCCTTCCGGTGAAAGCACAATGTCCACCTCTTCCTCACCTTCGGAAGCTTAATTTGGTTGGCAGACTGCAAAACGTAATACCTTGGTTTTCTTCACTGCATAGCCTCACATCTCTGGTTCTGCATTGGTCAAGACTTGAAGATGATTTACTACCTCAAATTGAAGCACTGTGCAATCTGACAAGGCTTTATCTTTGTAATGCATATGTTGGGGAAGAGTTGTGTTTCCGTAGAGGCTTTGTAAAGCTGATGAGGTTGGAGTTGTTGAATTTTGCCTCGGTGAAAAAGATAACTATAGAAAATGGGGTGATGCCAAATCTCCAGTACTTATGCGTTAACAGTTTCATGGAGTTAAAGACGGTGCCACTGGGCTTTGAATACCTTTCTACTCTACAATATTTGGGCCTGAAAGATGTTCCCATGGAAGTTATTAGGTCCATTCAGAAAGGAGGTGTGGATCACTATAAAGTACAACACATTCCTGAGATCATACATGTTTTTGAACAGTCATCCAAGTGGATCCACGAAAACTTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

920

Amino Acids

106.17

Weight (kDa)

8.76

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 90 1.8e-23 Rx N-terminal domain
NB-ARC PF00931 172 - 347 8.9e-46 NB-ARC domain
WHD_DRP PF23559 434 - 509 1.5e-18 Disease resistance protein Winged helix domain
LRR_14 PF23598 553 - 874 7.6e-38 Leucine-rich repeat region
LRR_8 PF13855 580 - 636 7.6e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 65, 1394
AasI GACNNNNNNGTC 1 cut(s) 2159
Acc36I ACCTGC 3 cut(s) 65, 1394, 1953
AccB1I GGYRCC 2 cut(s) 1406, 2584
AccB7I CCANNNNNTGG 1 cut(s) 1090
AccI GTMKAC 3 cut(s) 271, 1532, 1563
AccII CGCG 1 cut(s) 303
AccIII TCCGGA 2 cut(s) 1466, 1849
AciI CCGC 6 cut(s) 143, 349, 464, 775, 1870, 1948
AclWI GGATC 5 cut(s) 1170, 1477, 2688, 2737, 2750
AcsI RAATTY 5 cut(s) 233, 745, 1819, 2047, 2491
AcuI CTGAAG 4 cut(s) 186, 1088, 1295, 1413
AcyI GRCGYC 1 cut(s) 1864
AfaI GTAC 6 cut(s) 696, 915, 1985, 2114, 2549, 2694
AfiI CCNNNNNNNGG 2 cut(s) 1090, 1416
AflII CTTAAG 2 cut(s) 1496, 2108
AflIII ACRYGT 5 cut(s) 246, 843, 1689, 1797, 2716
AhlI ACTAGT 1 cut(s) 796
AjiI CACGTC 1 cut(s) 844
AjuI GAANNNNNNNTTGG 2 cut(s) 2261, 2293
AloI GAACNNNNNNTCC 2 cut(s) 1413, 1445
AluBI AGCT 9 cut(s) 128, 711, 1066, 1079, 1133, 1522, 1994, 2272, 2470
AluI AGCT 9 cut(s) 128, 711, 1066, 1079, 1133, 1522, 1994, 2272, 2470
Alw26I GTCTC 4 cut(s) 192, 637, 1871, 2167
AlwI GGATC 5 cut(s) 1170, 1477, 2688, 2737, 2750
AlwNI CAGNNNCTG 1 cut(s) 988
Aor13HI TCCGGA 2 cut(s) 1466, 1849
AoxI GGCC 7 cut(s) 3, 147, 204, 406, 1105, 2626, 2757
ApeKI GCWGC 2 cut(s) 1784, 1994
ApoI RAATTY 5 cut(s) 233, 745, 1819, 2047, 2491
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2226
AspLEI GCGC 2 cut(s) 305, 2014
AspS9I GGNCC 4 cut(s) 19, 1978, 2626, 2658
AsuHPI GGTGA 6 cut(s) 616, 1464, 2245, 2252, 2515, 2542
AvaII GGWCC 3 cut(s) 19, 1978, 2658
BaeGI GKGCMC 1 cut(s) 1832
BaeI ACNNNNGTAYC 2 cut(s) 686, 719
BamHI GGATCC 1 cut(s) 2742
BanI GGYRCC 2 cut(s) 1406, 2584
BarI GAAGNNNNNNTAC 2 cut(s) 2355, 2387
BbsI GAAGAC 2 cut(s) 462, 495
BbvI GCAGC 2 cut(s) 1771, 1981
BccI CCATC 4 cut(s) 528, 1273, 1448, 2023
BcgI CGANNNNNNTGC 4 cut(s) 1819, 1853, 2071, 2105
BclI TGATCA 2 cut(s) 366, 714
BcoDI GTCTC 4 cut(s) 192, 637, 1871, 2167
BcuI ACTAGT 1 cut(s) 796
BfmI CTRYAG 2 cut(s) 69, 2517
BfoI RGCGCY 1 cut(s) 2015
BfrI CTTAAG 2 cut(s) 1496, 2108
BfuAI ACCTGC 3 cut(s) 65, 1394, 1953
BglII AGATCT 1 cut(s) 1590
BisI GCNGC 3 cut(s) 775, 1785, 1995
BlsI GCNGC 3 cut(s) 776, 1786, 1996
BmcAI AGTACT 1 cut(s) 2549
Bme18I GGWCC 3 cut(s) 19, 1978, 2658
BmgBI CACGTC 1 cut(s) 844
BmgT120I GGNCC 4 cut(s) 19, 1978, 2626, 2658
BmiI GGNNCC 4 cut(s) 1408, 1979, 2586, 2744
BmrI ACTGGG 1 cut(s) 2600
BmsI GCATC 5 cut(s) 130, 950, 1491, 1503, 2523
BmuI ACTGGG 1 cut(s) 2600
BpiI GAAGAC 2 cut(s) 462, 495
BpmI CTGGAG 1 cut(s) 2528
BpuEI CTTGAG 2 cut(s) 71, 1828
BsaHI GRCGYC 1 cut(s) 1864
BsaI GGTCTC 1 cut(s) 2167
BsaJI CCNNGG 3 cut(s) 2306, 2499, 2643
BsaWI WCCGGW 3 cut(s) 1466, 1849, 2230
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 2 cut(s) 1090, 1416
Bse118I RCCGGY 1 cut(s) 1780
Bse1I ACTGG 6 cut(s) 141, 501, 1722, 2208, 2545, 2595
Bse3DI GCAATG 1 cut(s) 1279
BseAI TCCGGA 2 cut(s) 1466, 1849
BseDI CCNNGG 3 cut(s) 2306, 2499, 2643
BseGI GGATG 7 cut(s) 145, 428, 539, 550, 941, 1765, 2732
BseLI CCNNNNNNNGG 2 cut(s) 1090, 1416
BseMI GCAATG 1 cut(s) 1279
BseMII CTCAG 3 cut(s) 1053, 1478, 2697
BseNI ACTGG 6 cut(s) 141, 501, 1722, 2208, 2545, 2595
BseRI GAGGAG 2 cut(s) 122, 432
BseSI GKGCMC 1 cut(s) 1832
BseXI GCAGC 2 cut(s) 1771, 1981
BsgI GTGCAG 1 cut(s) 473
Bsh1236I CGCG 1 cut(s) 303
BshFI GGCC 7 cut(s) 5, 149, 206, 408, 1107, 2628, 2759
BshNI GGYRCC 2 cut(s) 1406, 2584
BsiSI CCGG 4 cut(s) 1467, 1781, 1850, 2231
BslFI GGGAC 3 cut(s) 1991, 2017, 2087
BslI CCNNNNNNNGG 2 cut(s) 1090, 1416
BsmAI GTCTC 4 cut(s) 192, 637, 1871, 2167
BsmBI CGTCTC 1 cut(s) 1871
BsmFI GGGAC 3 cut(s) 1991, 2017, 2087
BsmI GAATGC 2 cut(s) 769, 1028
BsnI GGCC 7 cut(s) 5, 149, 206, 408, 1107, 2628, 2759
Bso31I GGTCTC 1 cut(s) 2167
Bsp1286I GDGCHC 1 cut(s) 1832
Bsp13I TCCGGA 2 cut(s) 1466, 1849
Bsp143I GATC 9 cut(s) 366, 714, 1162, 1469, 1507, 1590, 2680, 2709, 2742
Bsp19I CCATGG 1 cut(s) 2643
BspACI CCGC 6 cut(s) 143, 349, 464, 775, 1870, 1948
BspANI GGCC 7 cut(s) 5, 149, 206, 408, 1107, 2628, 2759
BspCNI CTCAG 3 cut(s) 1054, 1479, 2698
BspEI TCCGGA 2 cut(s) 1466, 1849
BspFNI CGCG 1 cut(s) 303
BspHI TCATGA 1 cut(s) 85
BspLI GGNNCC 4 cut(s) 1408, 1979, 2586, 2744
BspMI ACCTGC 3 cut(s) 65, 1394, 1953
BspPI GGATC 5 cut(s) 1170, 1477, 2688, 2737, 2750
BspT107I GGYRCC 2 cut(s) 1406, 2584
BspTI CTTAAG 2 cut(s) 1496, 2108
BspTNI GGTCTC 1 cut(s) 2167
BsrDI GCAATG 1 cut(s) 1279
BsrFI RCCGGY 1 cut(s) 1780
BsrI ACTGG 6 cut(s) 141, 501, 1722, 2208, 2545, 2595
BssAI RCCGGY 1 cut(s) 1780
BssECI CCNNGG 3 cut(s) 2306, 2499, 2643
BssMI GATC 9 cut(s) 366, 714, 1162, 1469, 1507, 1590, 2680, 2709, 2742
BssNAI GTATAC 1 cut(s) 1564
BssNI GRCGYC 1 cut(s) 1864
BssT1I CCWWGG 2 cut(s) 2306, 2643
Bst1107I GTATAC 1 cut(s) 1564
Bst4CI ACNGT 7 cut(s) 401, 565, 673, 2394, 2564, 2584, 2730
Bst6I CTCTTC 6 cut(s) 1019, 1581, 1674, 2211, 2259, 2433
BstACI GRCGYC 1 cut(s) 1864
BstAFI CTTAAG 2 cut(s) 1496, 2108
BstAPI GCANNNNNTGC 1 cut(s) 2041
BstC8I GCNNGC 3 cut(s) 1081, 1524, 1782
BstDEI CTNAG 7 cut(s) 116, 1038, 1062, 1149, 1487, 1902, 2706
BstDSI CCRYGG 1 cut(s) 2643
BstF5I GGATG 7 cut(s) 145, 428, 539, 550, 941, 1765, 2732
BstFNI CGCG 1 cut(s) 303
BstH2I RGCGCY 1 cut(s) 2015
BstHHI GCGC 2 cut(s) 305, 2014
BstKTI GATC 9 cut(s) 369, 717, 1165, 1472, 1510, 1593, 2683, 2712, 2745
BstMAI GTCTC 4 cut(s) 192, 637, 1871, 2167
BstMBI GATC 9 cut(s) 366, 714, 1162, 1469, 1507, 1590, 2680, 2709, 2742
BstMWI GCNNNNNNNGC 4 cut(s) 309, 1434, 1945, 2041
BstNSI RCATGY 5 cut(s) 250, 1441, 1693, 1801, 2720
BstSFI CTRYAG 2 cut(s) 69, 2517
BstSLI GKGCMC 1 cut(s) 1832
BstUI CGCG 1 cut(s) 303
BstV1I GCAGC 2 cut(s) 1771, 1981
BstV2I GAAGAC 2 cut(s) 462, 495
BstX2I RGATCY 2 cut(s) 1590, 2742
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 2 cut(s) 1590, 2742
BstZ17I GTATAC 1 cut(s) 1564
BsuRI GGCC 7 cut(s) 5, 149, 206, 408, 1107, 2628, 2759
BtgI CCRYGG 1 cut(s) 2643
BtrI CACGTC 1 cut(s) 844
BtsCI GGATG 7 cut(s) 145, 428, 539, 550, 941, 1765, 2732
BtsI GCAGTG 1 cut(s) 2318
BtsIMutI CAGTG 6 cut(s) 1254, 1683, 1715, 2318, 2390, 2588
BveI ACCTGC 3 cut(s) 65, 1394, 1953
Cac8I GCNNGC 3 cut(s) 1081, 1524, 1782
CaiI CAGNNNCTG 1 cut(s) 988
CciI TCATGA 1 cut(s) 85
CfoI GCGC 2 cut(s) 305, 2014
Cfr10I RCCGGY 1 cut(s) 1780
Cfr13I GGNCC 4 cut(s) 19, 1978, 2626, 2658
CseI GACGC 1 cut(s) 1853
Csp6I GTAC 6 cut(s) 695, 914, 1984, 2113, 2548, 2693
CviQI GTAC 6 cut(s) 695, 914, 1984, 2113, 2548, 2693
DdeI CTNAG 7 cut(s) 116, 1038, 1062, 1149, 1487, 1902, 2706
DpnI GATC 9 cut(s) 368, 716, 1164, 1471, 1509, 1592, 2682, 2711, 2744
DpnII GATC 9 cut(s) 366, 714, 1162, 1469, 1507, 1590, 2680, 2709, 2742
DraI TTTAAA 1 cut(s) 1818
DrdI GACNNNNNNGTC 1 cut(s) 2159
DseDI GACNNNNNNGTC 1 cut(s) 2159
Eam1104I CTCTTC 6 cut(s) 1019, 1581, 1674, 2211, 2259, 2433
EarI CTCTTC 6 cut(s) 1019, 1581, 1674, 2211, 2259, 2433
Eco130I CCWWGG 2 cut(s) 2306, 2643
Eco147I AGGCCT 1 cut(s) 1107
Eco31I GGTCTC 1 cut(s) 2167
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 3 cut(s) 19, 1978, 2658
Eco57I CTGAAG 4 cut(s) 186, 1088, 1295, 1413
EcoO109I RGGNCCY 1 cut(s) 1978
EcoRI GAATTC 1 cut(s) 233
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 2 cut(s) 2306, 2643
EcoT22I ATGCAT 3 cut(s) 664, 1506, 2428
ErhI CCWWGG 2 cut(s) 2306, 2643
Esp3I CGTCTC 1 cut(s) 1871
FalI AAGNNNNNCTT 2 cut(s) 1268, 1300
FaqI GGGAC 3 cut(s) 1991, 2017, 2087
FauNDI CATATG 1 cut(s) 2428
FbaI TGATCA 2 cut(s) 366, 714
FblI GTMKAC 3 cut(s) 271, 1532, 1563
Fnu4HI GCNGC 3 cut(s) 775, 1785, 1995
FokI GGATG 7 cut(s) 152, 415, 546, 557, 928, 1772, 2719
Fsp4HI GCNGC 3 cut(s) 775, 1785, 1995
GlaI GCGC 2 cut(s) 304, 2013
GluI GCNGC 3 cut(s) 775, 1785, 1995
GsuI CTGGAG 1 cut(s) 2528
HaeII RGCGCY 1 cut(s) 2015
HaeIII GGCC 7 cut(s) 5, 149, 206, 408, 1107, 2628, 2759
HapII CCGG 4 cut(s) 1467, 1781, 1850, 2231
HgaI GACGC 1 cut(s) 1853
HhaI GCGC 2 cut(s) 305, 2014
Hin1I GRCGYC 1 cut(s) 1864
Hin6I GCGC 2 cut(s) 303, 2012
HinP1I GCGC 2 cut(s) 303, 2012
HincII GTYRAC 4 cut(s) 272, 802, 1533, 2560
HindII GTYRAC 4 cut(s) 272, 802, 1533, 2560
HindIII AAGCTT 3 cut(s) 126, 709, 2270
HpaI GTTAAC 2 cut(s) 802, 2560
HpaII CCGG 4 cut(s) 1467, 1781, 1850, 2231
HphI GGTGA 6 cut(s) 616, 1464, 2245, 2252, 2515, 2542
Hpy99I CGWCG 2 cut(s) 1571, 1605
HpyCH4III ACNGT 7 cut(s) 401, 565, 673, 2394, 2564, 2584, 2730
HpyCH4IV ACGT 2 cut(s) 843, 2298
HpyF10VI GCNNNNNNNGC 4 cut(s) 309, 1434, 1945, 2041
HpyF3I CTNAG 7 cut(s) 116, 1038, 1062, 1149, 1487, 1902, 2706
HpySE526I ACGT 2 cut(s) 843, 2298
Hsp92I GRCGYC 1 cut(s) 1864
HspAI GCGC 2 cut(s) 303, 2012
Kpn2I TCCGGA 2 cut(s) 1466, 1849
KroI GCCGGC 1 cut(s) 1780
KroNI GCCGGC 1 cut(s) 1782
Ksp22I TGATCA 2 cut(s) 366, 714
KspAI GTTAAC 2 cut(s) 802, 2560
Kzo9I GATC 9 cut(s) 366, 714, 1162, 1469, 1507, 1590, 2680, 2709, 2742
LmnI GCTCC 2 cut(s) 310, 419
Lsp1109I GCAGC 2 cut(s) 1771, 1981
LweI GCATC 5 cut(s) 130, 950, 1491, 1503, 2523
MaeII ACGT 2 cut(s) 843, 2298
MaeIII GTNAC 5 cut(s) 13, 792, 1384, 1440, 1911
MalI GATC 9 cut(s) 368, 716, 1164, 1471, 1509, 1592, 2682, 2711, 2744
MboI GATC 9 cut(s) 366, 714, 1162, 1469, 1507, 1590, 2680, 2709, 2742
MfeI CAATTG 1 cut(s) 1788
MflI RGATCY 2 cut(s) 1590, 2742
MhlI GDGCHC 1 cut(s) 1832
MlyI GAGTC 7 cut(s) 476, 821, 918, 1738, 1862, 1919, 2095
MmeI TCCRAC 3 cut(s) 969, 1082, 2460
Mph1103I ATGCAT 3 cut(s) 664, 1506, 2428
MroI TCCGGA 2 cut(s) 1466, 1849
MroNI GCCGGC 1 cut(s) 1780
MroXI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2226
MslI CAYNNNNRTG 3 cut(s) 249, 1688, 2737
MspCI CTTAAG 2 cut(s) 1496, 2108
MspI CCGG 4 cut(s) 1467, 1781, 1850, 2231
MunI CAATTG 1 cut(s) 1788
Mva1269I GAATGC 2 cut(s) 769, 1028
MvnI CGCG 1 cut(s) 303
MwoI GCNNNNNNNGC 4 cut(s) 309, 1434, 1945, 2041
NaeI GCCGGC 1 cut(s) 1782
NcoI CCATGG 1 cut(s) 2643
NdeI CATATG 1 cut(s) 2428
NdeII GATC 9 cut(s) 366, 714, 1162, 1469, 1507, 1590, 2680, 2709, 2742
NgoMIV GCCGGC 1 cut(s) 1780
NlaIV GGNNCC 4 cut(s) 1408, 1979, 2586, 2744
NmuCI GTSAC 2 cut(s) 1384, 1911
NsiI ATGCAT 3 cut(s) 664, 1506, 2428
NspI RCATGY 5 cut(s) 250, 1441, 1693, 1801, 2720
PagI TCATGA 1 cut(s) 85
PaqCI CACCTGC 2 cut(s) 65, 1394
PceI AGGCCT 1 cut(s) 1107
PciI ACATGT 4 cut(s) 246, 1689, 1797, 2716
PctI GAATGC 2 cut(s) 769, 1028
PdiI GCCGGC 1 cut(s) 1782
PdmI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2226
PfeI GAWTC 6 cut(s) 192, 388, 1660, 1728, 1951, 2063
PflMI CCANNNNNTGG 1 cut(s) 1090
PkrI GCNGC 3 cut(s) 776, 1786, 1996
PleI GAGTC 7 cut(s) 475, 820, 917, 1738, 1861, 1918, 2095
PpsI GAGTC 7 cut(s) 475, 820, 917, 1738, 1861, 1918, 2095
PpuMI RGGWCCY 1 cut(s) 1978
PscI ACATGT 4 cut(s) 246, 1689, 1797, 2716
Psp5II RGGWCCY 1 cut(s) 1978
PspN4I GGNNCC 4 cut(s) 1408, 1979, 2586, 2744
PspPI GGNCC 4 cut(s) 19, 1978, 2626, 2658
PspPPI RGGWCCY 1 cut(s) 1978
PstNI CAGNNNCTG 1 cut(s) 988
PsuI RGATCY 2 cut(s) 1590, 2742
RsaI GTAC 6 cut(s) 696, 915, 1985, 2114, 2549, 2694
RsaNI GTAC 6 cut(s) 695, 914, 1984, 2113, 2548, 2693
RseI CAYNNNNRTG 3 cut(s) 249, 1688, 2737
SalI GTCGAC 2 cut(s) 270, 1531
SatI GCNGC 3 cut(s) 775, 1785, 1995
Sau3AI GATC 9 cut(s) 366, 714, 1162, 1469, 1507, 1590, 2680, 2709, 2742
Sau96I GGNCC 4 cut(s) 19, 1978, 2626, 2658
ScaI AGTACT 1 cut(s) 2549
SchI GAGTC 7 cut(s) 476, 821, 918, 1738, 1862, 1919, 2095
SduI GDGCHC 1 cut(s) 1832
SfaNI GCATC 5 cut(s) 130, 950, 1491, 1503, 2523
SfcI CTRYAG 2 cut(s) 69, 2517
SinI GGWCC 3 cut(s) 19, 1978, 2658
SmiI ATTTAAAT 1 cut(s) 1818
SmiMI CAYNNNNRTG 3 cut(s) 249, 1688, 2737
SmlI CTYRAG 4 cut(s) 50, 1496, 1807, 2108
SmoI CTYRAG 4 cut(s) 50, 1496, 1807, 2108
SpeI ACTAGT 1 cut(s) 796
SseBI AGGCCT 1 cut(s) 1107
SsiI CCGC 6 cut(s) 143, 349, 464, 775, 1870, 1948
SspI AATATT 2 cut(s) 2023, 2621
StuI AGGCCT 1 cut(s) 1107
StyI CCWWGG 2 cut(s) 2306, 2643
SwaI ATTTAAAT 1 cut(s) 1818
TaaI ACNGT 7 cut(s) 401, 565, 673, 2394, 2564, 2584, 2730
TaiI ACGT 2 cut(s) 846, 2301
TaqI TCGA 6 cut(s) 271, 907, 925, 1532, 1726, 1839
TatI WGTACW 4 cut(s) 1983, 2112, 2547, 2692
TauI GCSGC 1 cut(s) 777
TfiI GAWTC 6 cut(s) 192, 388, 1660, 1728, 1951, 2063
TscAI CASTG 6 cut(s) 1261, 1690, 1722, 2325, 2397, 2595
TseFI GTSAC 2 cut(s) 1384, 1911
TseI GCWGC 2 cut(s) 1784, 1994
Tsp45I GTSAC 2 cut(s) 1384, 1911
TspGWI ACGGA 3 cut(s) 32, 1421, 2441
TspRI CASTG 6 cut(s) 1261, 1690, 1722, 2325, 2397, 2595
Van91I CCANNNNNTGG 1 cut(s) 1090
Vha464I CTTAAG 2 cut(s) 1496, 2108
VpaK11BI GGWCC 3 cut(s) 19, 1978, 2658
XapI RAATTY 5 cut(s) 233, 745, 1819, 2047, 2491
XbaI TCTAGA 3 cut(s) 835, 1219, 1747
XceI RCATGY 5 cut(s) 250, 1441, 1693, 1801, 2720
XcmI CCANNNNNNNNNTGG 1 cut(s) 315
XmiI GTMKAC 3 cut(s) 271, 1532, 1563
XmnI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2226
ZrmI AGTACT 1 cut(s) 2549
Zsp2I ATGCAT 3 cut(s) 664, 1506, 2428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.