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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 114 |
| AcoI | YGGCCR | 1 cut(s) | 122 |
| AfaI | GTAC | 1 cut(s) | 18 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 103 |
| AgsI | TTSAA | 1 cut(s) | 26 |
| AluBI | AGCT | 1 cut(s) | 94 |
| AluI | AGCT | 1 cut(s) | 94 |
| AoxI | GGCC | 1 cut(s) | 122 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 103 |
| Bse1I | ACTGG | 1 cut(s) | 108 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 103 |
| BseNI | ACTGG | 1 cut(s) | 108 |
| BseRI | GAGGAG | 1 cut(s) | 120 |
| BshFI | GGCC | 1 cut(s) | 124 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 103 |
| BsnI | GGCC | 1 cut(s) | 124 |
| Bsp1407I | TGTACA | 1 cut(s) | 16 |
| BspANI | GGCC | 1 cut(s) | 124 |
| BsrGI | TGTACA | 1 cut(s) | 16 |
| BsrI | ACTGG | 1 cut(s) | 108 |
| BstAUI | TGTACA | 1 cut(s) | 16 |
| BsuRI | GGCC | 1 cut(s) | 124 |
| Csp6I | GTAC | 1 cut(s) | 17 |
| CviJI | RGCY | 2 cut(s) | 94, 124 |
| CviKI_1 | RGCY | 2 cut(s) | 94, 124 |
| CviQI | GTAC | 1 cut(s) | 17 |
| EaeI | YGGCCR | 1 cut(s) | 122 |
| FaiI | YATR | 5 cut(s) | 21, 71, 81, 114, 149 |
| HaeIII | GGCC | 1 cut(s) | 124 |
| HpyAV | CCTTC | 1 cut(s) | 20 |
| LmnI | GCTCC | 1 cut(s) | 99 |
| LpnPI | CCDG | 1 cut(s) | 89 |
| MboII | GAAGA | 1 cut(s) | 16 |
| MfeI | CAATTG | 1 cut(s) | 12 |
| MluCI | AATT | 1 cut(s) | 12 |
| MnlI | CCTC | 2 cut(s) | 54, 141 |
| MslI | CAYNNNNRTG | 1 cut(s) | 148 |
| MunI | CAATTG | 1 cut(s) | 12 |
| PsiI | TTATAA | 1 cut(s) | 114 |
| RsaI | GTAC | 1 cut(s) | 18 |
| RsaNI | GTAC | 1 cut(s) | 17 |
| RseI | CAYNNNNRTG | 1 cut(s) | 148 |
| SetI | ASST | 1 cut(s) | 96 |
| SgeI | CNNG | 2 cut(s) | 116, 144 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 148 |
| Sse9I | AATT | 1 cut(s) | 12 |
| TasI | AATT | 1 cut(s) | 12 |
| TatI | WGTACW | 1 cut(s) | 16 |
| TspDTI | ATGAA | 2 cut(s) | 17, 58 |