Rorug06G0081600

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000110
Physical Location & Seq
Forward (+)
10880028 .. 10883077
3050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0081600.1

Sequence Viewer

Length: 2463 bp
ATGTTCAGTTTGTTGCCCACCTGCAAAGGTAGGCGTGCTTGCAAAAGTTTCCCCTCACTGCGAGTCTGTTACCAATTCTTCTCATCAGCTACTAATTCATTGCCTTCTGTATCGGATGGCTTTGGAACTGAGAAAAAAAGAACTGATTTCGGTCTTCTATTTCAATCCTGCACAAAACTTCACCATGCGAAGTGTCTCCATGCTTTTCTTGTGGTGTCAGGAAAAGCTCAAGATATTATTTTTTCCTCCAGGCTAGTCAATCGGTATGCCTACATTGGTGATGTTTCATTCTCACGCCGCACTTTTGATTCAATGCCTAGAAAGGATGTCTATACCTGGAATTCAATGGTGTCTGCATATGTTCGCAGTGGACGTTTCCGAGAAGCTGTAGACTGTTTCTCTGATTTTTTGTTGACCCCTGGTCTGCGACCTGATTTCTACACTTTTCCTCCTGTCTTGAAAGCGTGTGGGAATCTAAATGTTGGGAAGAAGATACACTGTTGGCTTATAAAGCTGGGTTTGGAATGGGATGTCTTTGTGGCTGCTTCCTTGATCCATATGTATTCACGGTTTGGATTTATTAGCGTTGCTCATAAATTGTTTGATGAGATGCCATTCCGGGATATGGGTTGTTGGAATGCAATGATTTCTGGGTTTTGTCAGAATGGGAATGCAGCAGATGCATTAGATGTCTTGATTGAAATGAGATCGGAGGGTGTAAAGATGGATCCTGTAACTGTTGCAAGTCTACTGACTGCTTGTGCACAATCAGATGATTCCTTAAGTGGAATGCTGATTCATTTGTATGTCATAAAGCATGGACTAGAGTTTGATCTGTTCGTATGCAATGCCTTGATTAACATGTATGCTAAATTTGGTTGCTTGGGACAAGCACACAGGGTTTTTTATGAAATGCATGTAAGAGATTTGGTGTCATGGAACTCAATAATTACTGCAAATGAGCAGAATGATGAGCCGATGACTGCACTTGGATTCTTGAACAGGATGCAACTATCTGGAGTTAAGCCTGATTGGTTAACACTAGTGAGTTTGGCTTCCATTATAGCTCAATTAAATGATGCTGAAATGAGTAGGTCTGTTCATGGATTCATTTTGAGGAGAGATTGGTTCAAGGAAGATGTTGTTATTGGAAATGCAGTTGTGGACATGTATGCAAAGTTGGGTGCTATAGATTCCGCACGTACTGTTTTTGAAGGACTTCCTGTCAAGGATGTGGTTTCATGGAATACTTTGATCACAGGTTACACTCAAAATGGTCTTGCAAGTGAGGCGAATGAAGTGTACTGTATGATGCAAGAGTGCAAAGAAATAATTCCAAACCAAGGAAGTTGGGTTAGCATTCTACCAGCATATACCCATTTAGGGGCCTTGCAACAAGGGATGAGAATTCATGGGCGGGTGATCAAGAACTGTCTCTACTTGGATGTATTTGTGGGTACCTGCTTGATTGACATGTACGGAAAATGTGGGAGACTAGATGATGCATTATCATTATTCTACCAAGTGCCCAGAATGAGTTCAGTCACTTGGAATGCCATGATTTCCTGTCTTGGAGTTCATGGGCATGGTGGGAAAGCTGTGAAGCTATTCAAAGATATGATAGATGAGGGGGTCAAGCCAGATCATGTAACCTTTGTATCTCTAATGGCAGCTTGTAGTCATTCAGGTTTGGTCAATGAGGGTGAATTGTACTTTCATATAATGCAGAAAGAGTATGGAATCAAGCCTGGTTTAAAGCACTACGGCTGCATGGTAGATTTACTTGGCAGAGCTGGGCATTTAGACAAGGCATATAGTTTTATTAAGACTATGCCTGTACAGCCTGATGCTTCTGTTTGGGGGGCACTACTTGGTGCTTGTAGAATACATGGGAATGCTGAATTGGGGAAAATTGCTTCAGAAGGCTTGTTTGAAGTTGATTCGGAGAATGTGGGCTACTATGTTTTGTTGTCAAATATATACGCAACAACTGGGAAATGGGAAGGTGTGGATAAAGTGAGATCTATGGCTAGAAACCGCAGACTGAGGAAGACTCCCGGGTGGAGCTCAATAGAAGTTAACAATAAAGTTGATGTGTTTTATACTGGAAACCAAACGCATCCATTATGCGAACAGATATACCAGAAGTTGAGGGAACTAACTGAGAAAATGAAGAGCCTTGGTTATGTTCCGGACTTCAGCTTTGTGTTGCAGGACGTCGAGGATGACGAGAAAGAGCATATCCTCATGAGTCATAGTGAGAGATTGGCGATTGCTTTTGGAATCATTAGCACGCCTCCTAAAACTCCCATTCGGATCTTCAAGAACCTGAGGGTTTGCGGTGATTGCCACAATGTGACGAAACTCATATCAGTAATTACGGAGAGGGAGATCATTGTGAGGGATTCAAACCGTTTCCACCATTTCAAGGATGGAACTTGCTCTTGTGGGGATTATTGGTGA

Protein Analysis

820

Amino Acids

92.08

Weight (kDa)

7.71

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 108 - 156 2e-06 PPR repeat family
PPR PF01535 111 - 135 8.6e-06 PPR repeat
PPR_2 PF13041 208 - 255 1.1e-10 PPR repeat family
PPR_1 PF12854 208 - 236 3.7e-06 PPR repeat
PPR PF01535 211 - 240 5.9e-08 PPR repeat
TPR_24 PF23276 213 - 303 6.8e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 410 - 452 1.8e-06 PPR repeat family
PPR PF01535 413 - 441 1.2e-06 PPR repeat
PPR_2 PF13041 513 - 560 1.1e-12 PPR repeat family
PPR PF01535 515 - 545 8.7e-08 PPR repeat
E_motif PF20431 630 - 692 3.4e-16 E motif
DYW_deaminase PF14432 728 - 820 4.7e-45 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 509
AarI CACCTGC 1 cut(s) 29
AatII GACGTC 1 cut(s) 2220
Acc36I ACCTGC 2 cut(s) 29, 1469
Acc65I GGTACC 1 cut(s) 1457
AccB1I GGYRCC 1 cut(s) 1457
AccI GTMKAC 2 cut(s) 390, 748
AccIII TCCGGA 1 cut(s) 2191
AciI CCGC 5 cut(s) 298, 1197, 1417, 2038, 2340
AclWI GGATC 4 cut(s) 547, 722, 735, 2324
AcsI RAATTY 3 cut(s) 340, 872, 1407
AcuI CTGAAG 2 cut(s) 1902, 2182
AcyI GRCGYC 1 cut(s) 2217
AdeI CACNNNGTG 1 cut(s) 2356
AfaI GTAC 6 cut(s) 1204, 1304, 1459, 1478, 1712, 1839
AfiI CCNNNNNNNGG 5 cut(s) 625, 1343, 1383, 1384, 2428
AflII CTTAAG 1 cut(s) 781
AflIII ACRYGT 3 cut(s) 861, 1167, 1473
AhdI GACNNNNNGTC 2 cut(s) 420, 1223
AhlI ACTAGT 1 cut(s) 1042
AjnI CCWGG 4 cut(s) 248, 335, 418, 1747
AjuI GAANNNNNNNTTGG 2 cut(s) 1886, 1918
Alw21I GWGCWC 2 cut(s) 766, 2069
Alw26I GTCTC 3 cut(s) 200, 1439, 1486
Alw44I GTGCAC 1 cut(s) 762
AlwI GGATC 4 cut(s) 547, 722, 735, 2324
Ama87I CYCGRG 1 cut(s) 2057
Aor13HI TCCGGA 1 cut(s) 2191
AoxI GGCC 1 cut(s) 1386
ApaLI GTGCAC 1 cut(s) 762
ApeKI GCWGC 4 cut(s) 542, 674, 1670, 1767
ApoI RAATTY 3 cut(s) 340, 872, 1407
ArsI GACNNNNNNTTYG 2 cut(s) 2186, 2218
Asp700I GAANNNNTTC 4 cut(s) 1218, 1332, 1537, 1607
Asp718I GGTACC 1 cut(s) 1457
AspS9I GGNCC 1 cut(s) 1386
AsuC2I CCSGG 3 cut(s) 620, 2058, 2059
AsuHPI GGTGA 5 cut(s) 173, 290, 1432, 1715, 2354
AvaI CYCGRG 1 cut(s) 2057
AxyI CCTNAGG 1 cut(s) 2330
BaeGI GKGCMC 3 cut(s) 766, 1530, 1867
BaeI ACNNNNGTAYC 2 cut(s) 1641, 1674
BamHI GGATCC 1 cut(s) 727
BanI GGYRCC 1 cut(s) 1457
BanII GRGCYC 1 cut(s) 2069
BbsI GAAGAC 2 cut(s) 146, 2057
Bbv12I GWGCWC 2 cut(s) 766, 2069
BbvI GCAGC 4 cut(s) 529, 686, 1682, 1754
BccI CCATC 3 cut(s) 110, 718, 2426
BceAI ACGGC 1 cut(s) 1780
BciT130I CCWGG 4 cut(s) 250, 337, 420, 1749
BclI TGATCA 2 cut(s) 1254, 1422
BcnI CCSGG 3 cut(s) 620, 2058, 2059
BcoDI GTCTC 3 cut(s) 200, 1439, 1486
BcuI ACTAGT 1 cut(s) 1042
BfaI CTAG 6 cut(s) 254, 318, 824, 1043, 1496, 2031
BfmI CTRYAG 2 cut(s) 387, 1188
BfrI CTTAAG 1 cut(s) 781
BfuAI ACCTGC 2 cut(s) 29, 1469
BglII AGATCT 1 cut(s) 2021
BisI GCNGC 5 cut(s) 298, 543, 675, 1671, 1768
BlsI GCNGC 5 cut(s) 299, 544, 676, 1672, 1769
Bme1390I CCNGG 7 cut(s) 250, 337, 420, 620, 1749, 2058, 2059
BmeRI GACNNNNNGTC 2 cut(s) 420, 1223
BmeT110I CYCGRG 1 cut(s) 2057
BmgT120I GGNCC 1 cut(s) 1386
BmiI GGNNCC 3 cut(s) 729, 1387, 1459
BmrFI CCNGG 7 cut(s) 250, 337, 420, 620, 1749, 2058, 2059
BmrI ACTGGG 1 cut(s) 2001
BmsI GCATC 8 cut(s) 600, 670, 996, 1069, 1302, 1492, 1837, 2128
BmuI ACTGGG 1 cut(s) 2001
BpiI GAAGAC 2 cut(s) 146, 2057
BplI GAGNNNNNCTC 2 cut(s) 2038, 2070
BpmI CTGGAG 2 cut(s) 232, 1038
BpuEI CTTGAG 1 cut(s) 213
BpuMI CCSGG 3 cut(s) 620, 2058, 2059
BsaAI YACGTR 1 cut(s) 1202
BsaHI GRCGYC 1 cut(s) 2217
BsaJI CCNNGG 4 cut(s) 418, 1342, 2057, 2179
BsaWI WCCGGW 1 cut(s) 2191
BsaXI ACNNNNNCTCC 2 cut(s) 433, 463
Bsc4I CCNNNNNNNGG 5 cut(s) 625, 1343, 1383, 1384, 2428
Bse1I ACTGG 2 cut(s) 1996, 2110
Bse21I CCTNAGG 1 cut(s) 2330
Bse3DI GCAATG 3 cut(s) 98, 648, 853
BseAI TCCGGA 1 cut(s) 2191
BseBI CCWGG 4 cut(s) 250, 337, 420, 1749
BseDI CCNNGG 4 cut(s) 418, 1342, 2057, 2179
BseLI CCNNNNNNNGG 5 cut(s) 625, 1343, 1383, 1384, 2428
BseMI GCAATG 3 cut(s) 98, 648, 853
BseMII CTCAG 4 cut(s) 120, 2036, 2154, 2321
BseNI ACTGG 2 cut(s) 1996, 2110
BseRI GAGGAG 1 cut(s) 1132
BseSI GKGCMC 3 cut(s) 766, 1530, 1867
BseXI GCAGC 4 cut(s) 529, 686, 1682, 1754
BseYI CCCAGC 2 cut(s) 514, 1793
BsgI GTGCAG 2 cut(s) 154, 969
BshFI GGCC 1 cut(s) 1388
BshNI GGYRCC 1 cut(s) 1457
BsiHKAI GWGCWC 2 cut(s) 766, 2069
BsiHKCI CYCGRG 1 cut(s) 2057
BsiSI CCGG 3 cut(s) 619, 2058, 2192
BslFI GGGAC 1 cut(s) 900
BslI CCNNNNNNNGG 5 cut(s) 625, 1343, 1383, 1384, 2428
BsmAI GTCTC 3 cut(s) 200, 1439, 1486
BsmFI GGGAC 1 cut(s) 900
BsmI GAATGC 6 cut(s) 643, 676, 795, 1359, 1558, 1900
BsnI GGCC 1 cut(s) 1388
BsoBI CYCGRG 1 cut(s) 2057
Bsp1286I GDGCHC 4 cut(s) 766, 1530, 1867, 2069
Bsp13I TCCGGA 1 cut(s) 2191
Bsp1407I TGTACA 1 cut(s) 1837
BspACI CCGC 5 cut(s) 298, 1197, 1417, 2038, 2340
BspANI GGCC 1 cut(s) 1388
BspCNI CTCAG 4 cut(s) 121, 2037, 2155, 2322
BspEI TCCGGA 1 cut(s) 2191
BspHI TCATGA 1 cut(s) 2247
BspLI GGNNCC 3 cut(s) 729, 1387, 1459
BspMI ACCTGC 2 cut(s) 29, 1469
BspPI GGATC 4 cut(s) 547, 722, 735, 2324
BspQI GCTCTTC 1 cut(s) 2168
BspT107I GGYRCC 1 cut(s) 1457
BspTI CTTAAG 1 cut(s) 781
BsrDI GCAATG 3 cut(s) 98, 648, 853
BsrGI TGTACA 1 cut(s) 1837
BsrI ACTGG 2 cut(s) 1996, 2110
BssECI CCNNGG 4 cut(s) 418, 1342, 2057, 2179
BssNI GRCGYC 1 cut(s) 2217
BssT1I CCWWGG 2 cut(s) 1342, 2179
Bst2UI CCWGG 4 cut(s) 250, 337, 420, 1749
Bst4CI ACNGT 8 cut(s) 395, 500, 570, 739, 1207, 1307, 1433, 2414
Bst6I CTCTTC 1 cut(s) 2168
BstACI GRCGYC 1 cut(s) 2217
BstAFI CTTAAG 1 cut(s) 781
BstAPI GCANNNNNTGC 1 cut(s) 680
BstAUI TGTACA 1 cut(s) 1837
BstBAI YACGTR 1 cut(s) 1202
BstC8I GCNNGC 3 cut(s) 36, 40, 2294
BstDEI CTNAG 4 cut(s) 129, 2045, 2163, 2330
BstMAI GTCTC 3 cut(s) 200, 1439, 1486
BstMWI GCNNNNNNNGC 5 cut(s) 511, 680, 1289, 1840, 2346
BstNI CCWGG 4 cut(s) 250, 337, 420, 1749
BstNSI RCATGY 4 cut(s) 865, 920, 1171, 1477
BstSCI CCNGG 7 cut(s) 248, 335, 418, 618, 1747, 2056, 2057
BstSFI CTRYAG 2 cut(s) 387, 1188
BstSLI GKGCMC 3 cut(s) 766, 1530, 1867
BstV1I GCAGC 4 cut(s) 529, 686, 1682, 1754
BstV2I GAAGAC 2 cut(s) 146, 2057
BstX2I RGATCY 3 cut(s) 727, 2021, 2316
BstYI RGATCY 3 cut(s) 727, 2021, 2316
Bsu36I CCTNAGG 1 cut(s) 2330
BsuRI GGCC 1 cut(s) 1388
BtsI GCAGTG 2 cut(s) 56, 373
BtsIMutI CAGTG 3 cut(s) 56, 373, 496
BveI ACCTGC 2 cut(s) 29, 1469
Cac8I GCNNGC 3 cut(s) 36, 40, 2294
CciI TCATGA 1 cut(s) 2247
Cfr13I GGNCC 1 cut(s) 1386
Cfr9I CCCGGG 1 cut(s) 2057
Csp6I GTAC 6 cut(s) 1203, 1303, 1458, 1477, 1711, 1838
CviQI GTAC 6 cut(s) 1203, 1303, 1458, 1477, 1711, 1838
DdeI CTNAG 4 cut(s) 129, 2045, 2163, 2330
DraI TTTAAA 1 cut(s) 1755
DraIII CACNNNGTG 1 cut(s) 2356
DriI GACNNNNNGTC 2 cut(s) 420, 1223
Eam1104I CTCTTC 1 cut(s) 2168
Eam1105I GACNNNNNGTC 2 cut(s) 420, 1223
EarI CTCTTC 1 cut(s) 2168
Ecl136II GAGCTC 1 cut(s) 2067
Eco130I CCWWGG 2 cut(s) 1342, 2179
Eco24I GRGCYC 1 cut(s) 2069
Eco53kI GAGCTC 1 cut(s) 2067
Eco57I CTGAAG 2 cut(s) 1902, 2182
Eco81I CCTNAGG 1 cut(s) 2330
Eco88I CYCGRG 1 cut(s) 2057
EcoICRI GAGCTC 1 cut(s) 2067
EcoO109I RGGNCCY 1 cut(s) 1386
EcoRI GAATTC 2 cut(s) 340, 1407
EcoRII CCWGG 4 cut(s) 248, 335, 418, 1747
EcoT14I CCWWGG 2 cut(s) 1342, 2179
EcoT22I ATGCAT 3 cut(s) 685, 918, 1507
EcoT38I GRGCYC 1 cut(s) 2069
ErhI CCWWGG 2 cut(s) 1342, 2179
FaqI GGGAC 1 cut(s) 900
FauI CCCGC 1 cut(s) 1410
FauNDI CATATG 2 cut(s) 358, 558
FbaI TGATCA 2 cut(s) 1254, 1422
FblI GTMKAC 2 cut(s) 390, 748
Fnu4HI GCNGC 5 cut(s) 298, 543, 675, 1671, 1768
FriOI GRGCYC 1 cut(s) 2069
Fsp4HI GCNGC 5 cut(s) 298, 543, 675, 1671, 1768
FspBI CTAG 6 cut(s) 254, 318, 824, 1043, 1496, 2031
GluI GCNGC 5 cut(s) 298, 543, 675, 1671, 1768
GsaI CCCAGC 2 cut(s) 518, 1797
GsuI CTGGAG 2 cut(s) 232, 1038
HaeIII GGCC 1 cut(s) 1388
HapII CCGG 3 cut(s) 619, 2058, 2192
Hin1I GRCGYC 1 cut(s) 2217
HincII GTYRAC 3 cut(s) 414, 1038, 2080
HindII GTYRAC 3 cut(s) 414, 1038, 2080
HpaI GTTAAC 2 cut(s) 1038, 2080
HpaII CCGG 3 cut(s) 619, 2058, 2192
HphI GGTGA 5 cut(s) 173, 290, 1432, 1715, 2354
Hpy166II GTNNAC 9 cut(s) 371, 391, 414, 749, 764, 1038, 1165, 1303, 2080
Hpy188I TCNGA 9 cut(s) 115, 380, 403, 663, 712, 772, 1921, 1945, 2316
Hpy8I GTNNAC 9 cut(s) 371, 391, 414, 749, 764, 1038, 1165, 1303, 2080
Hpy99I CGWCG 1 cut(s) 2222
HpyAV CCTTC 4 cut(s) 114, 1208, 1916, 1997
HpyCH4III ACNGT 8 cut(s) 395, 500, 570, 739, 1207, 1307, 1433, 2414
HpyCH4IV ACGT 3 cut(s) 373, 1201, 2217
HpyF10VI GCNNNNNNNGC 5 cut(s) 511, 680, 1289, 1840, 2346
HpyF3I CTNAG 4 cut(s) 129, 2045, 2163, 2330
HpySE526I ACGT 3 cut(s) 373, 1201, 2217
Hsp92I GRCGYC 1 cut(s) 2217
Kpn2I TCCGGA 1 cut(s) 2191
KpnI GGTACC 1 cut(s) 1461
Ksp22I TGATCA 2 cut(s) 1254, 1422
KspAI GTTAAC 2 cut(s) 1038, 2080
LguI GCTCTTC 1 cut(s) 2168
LmnI GCTCC 1 cut(s) 2064
Lsp1109I GCAGC 4 cut(s) 529, 686, 1682, 1754
LweI GCATC 8 cut(s) 600, 670, 996, 1069, 1302, 1492, 1837, 2128
MaeI CTAG 6 cut(s) 254, 318, 824, 1043, 1496, 2031
MaeII ACGT 3 cut(s) 373, 1201, 2217
MaeIII GTNAC 6 cut(s) 68, 733, 1262, 1543, 1648, 2356
MboII GAAGA 8 cut(s) 70, 146, 499, 502, 1148, 2062, 2185, 2311
MflI RGATCY 3 cut(s) 727, 2021, 2316
MhlI GDGCHC 4 cut(s) 766, 1530, 1867, 2069
MlyI GAGTC 3 cut(s) 72, 2047, 2260
MmeI TCCRAC 1 cut(s) 614
Mph1103I ATGCAT 3 cut(s) 685, 918, 1507
MroI TCCGGA 1 cut(s) 2191
MroXI GAANNNNTTC 4 cut(s) 1218, 1332, 1537, 1607
MseI TTAA 8 cut(s) 782, 858, 1023, 1037, 1073, 1754, 1824, 2079
MslI CAYNNNNRTG 3 cut(s) 804, 1584, 1893
MspCI CTTAAG 1 cut(s) 781
MspI CCGG 3 cut(s) 619, 2058, 2192
MspR9I CCNGG 7 cut(s) 250, 337, 420, 620, 1749, 2058, 2059
Mva1269I GAATGC 6 cut(s) 643, 676, 795, 1359, 1558, 1900
MvaI CCWGG 4 cut(s) 250, 337, 420, 1749
MwoI GCNNNNNNNGC 5 cut(s) 511, 680, 1289, 1840, 2346
NciI CCSGG 3 cut(s) 620, 2058, 2059
NdeI CATATG 2 cut(s) 358, 558
NlaIV GGNNCC 3 cut(s) 729, 1387, 1459
NmuCI GTSAC 2 cut(s) 1543, 2356
NsiI ATGCAT 3 cut(s) 685, 918, 1507
NspI RCATGY 4 cut(s) 865, 920, 1171, 1477
PagI TCATGA 1 cut(s) 2247
PaqCI CACCTGC 1 cut(s) 29
PciI ACATGT 3 cut(s) 861, 1167, 1473
PciSI GCTCTTC 1 cut(s) 2168
PcsI WCGNNNNNNNCGW 2 cut(s) 370, 2226
PctI GAATGC 6 cut(s) 643, 676, 795, 1359, 1558, 1900
PdmI GAANNNNTTC 4 cut(s) 1218, 1332, 1537, 1607
PfoI TCCNGGA 1 cut(s) 618
PkrI GCNGC 5 cut(s) 299, 544, 676, 1672, 1769
PleI GAGTC 3 cut(s) 71, 2047, 2259
PpsI GAGTC 3 cut(s) 71, 2047, 2259
Ppu21I YACGTR 1 cut(s) 1202
PscI ACATGT 3 cut(s) 861, 1167, 1473
PsiI TTATAA 1 cut(s) 509
Psp124BI GAGCTC 1 cut(s) 2069
Psp6I CCWGG 4 cut(s) 248, 335, 418, 1747
PspFI CCCAGC 2 cut(s) 514, 1793
PspGI CCWGG 4 cut(s) 248, 335, 418, 1747
PspN4I GGNNCC 3 cut(s) 729, 1387, 1459
PspPI GGNCC 1 cut(s) 1386
PsrI GAACNNNNNNTAC 2 cut(s) 1421, 1453
PsuI RGATCY 3 cut(s) 727, 2021, 2316
RsaI GTAC 6 cut(s) 1204, 1304, 1459, 1478, 1712, 1839
RsaNI GTAC 6 cut(s) 1203, 1303, 1458, 1477, 1711, 1838
RseI CAYNNNNRTG 3 cut(s) 804, 1584, 1893
SacI GAGCTC 1 cut(s) 2069
SapI GCTCTTC 1 cut(s) 2168
SaqAI TTAA 8 cut(s) 782, 858, 1023, 1037, 1073, 1754, 1824, 2079
SatI GCNGC 5 cut(s) 298, 543, 675, 1671, 1768
Sau96I GGNCC 1 cut(s) 1386
SchI GAGTC 3 cut(s) 72, 2047, 2260
ScrFI CCNGG 7 cut(s) 250, 337, 420, 620, 1749, 2058, 2059
SduI GDGCHC 4 cut(s) 766, 1530, 1867, 2069
SfaNI GCATC 8 cut(s) 600, 670, 996, 1069, 1302, 1492, 1837, 2128
SfcI CTRYAG 2 cut(s) 387, 1188
SmaI CCCGGG 1 cut(s) 2059
SmiMI CAYNNNNRTG 3 cut(s) 804, 1584, 1893
SmlI CTYRAG 2 cut(s) 228, 781
SmoI CTYRAG 2 cut(s) 228, 781
SpeI ACTAGT 1 cut(s) 1042
SsiI CCGC 5 cut(s) 298, 1197, 1417, 2038, 2340
SspMI CTAG 6 cut(s) 254, 318, 824, 1043, 1496, 2031
SstI GAGCTC 1 cut(s) 2069
StyD4I CCNGG 7 cut(s) 248, 335, 418, 618, 1747, 2056, 2057
StyI CCWWGG 2 cut(s) 1342, 2179
TaaI ACNGT 8 cut(s) 395, 500, 570, 739, 1207, 1307, 1433, 2414
TaiI ACGT 3 cut(s) 376, 1204, 2220
TaqI TCGA 1 cut(s) 2220
TaqII GACCGA 1 cut(s) 140
TatI WGTACW 3 cut(s) 1302, 1710, 1837
TauI GCSGC 1 cut(s) 300
Tru1I TTAA 8 cut(s) 782, 858, 1023, 1037, 1073, 1754, 1824, 2079
Tru9I TTAA 8 cut(s) 782, 858, 1023, 1037, 1073, 1754, 1824, 2079
TscAI CASTG 3 cut(s) 63, 373, 503
TseFI GTSAC 2 cut(s) 1543, 2356
TseI GCWGC 4 cut(s) 542, 674, 1670, 1767
Tsp45I GTSAC 2 cut(s) 1543, 2356
TspGWI ACGGA 2 cut(s) 1494, 2396
TspMI CCCGGG 1 cut(s) 2057
TspRI CASTG 3 cut(s) 63, 373, 503
Vha464I CTTAAG 1 cut(s) 781
VneI GTGCAC 1 cut(s) 762
XapI RAATTY 3 cut(s) 340, 872, 1407
XceI RCATGY 4 cut(s) 865, 920, 1171, 1477
XcmI CCANNNNNNNNNTGG 1 cut(s) 2429
XmaI CCCGGG 1 cut(s) 2057
XmiI GTMKAC 2 cut(s) 390, 748
XmnI GAANNNNTTC 4 cut(s) 1218, 1332, 1537, 1607
XspI CTAG 6 cut(s) 254, 318, 824, 1043, 1496, 2031
ZraI GACGTC 1 cut(s) 2218
Zsp2I ATGCAT 3 cut(s) 685, 918, 1507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.