Prupe.8G008900_v2.0.a1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000110
Physical Location & Seq
Forward (+)
657539 .. 660467
2929 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G008900.1

Sequence Viewer

Length: 2700 bp
ATGGCCTCAACTGCAACCGATCTCTTGATTGGCAAAGTTGCAGGCATCCTTGAGAGCGAAGCATCCTCCATAGTAGGTGTTCGTGATCAAGTTGATGAGATTAAGCAAGAGCTTATAAGCATGAAATCCTTCCTAAAGGATGCTGAGGGGAAGAAACCGCAGACAGAAGGAGAGGAGACATGGGTAGCTAGCGTGAGAGATTTGGCCTATGATGTTGAAGATATCATTGATGAGTTCATGGGTCGATTTGCAAGCTGCCTCCACAAAGCCATTCACATTCCAAAGAAACTTTGGTATAGGCGTCAAATTGGCAAAAAGTTACAGAAAATCACAAAAACCATCAAGGACATTACAGAGAGGAATCAAAGATACGATATTGATCCCCTAGAAGGAACAAGTTCAGATGACATTAAAAAATGGGTGAAAAACCAAGCAGAGTCTTCCCTTTTTATTAAGGAAGACGAACTTGTGGGGATTGAAGACAAGAAGCAAATATTGATGGGATGGCTGATGAACGGAGAGCAACAACAAGCGGTTATCTCTGTGGTCGGAATGGGAGGATCAGGGAAGACAACTCTAGTTGCAAAAACCTTCCCCAGTGAAAGTGTGAAGAGACATTTCAGCTGTTATGCGTGGATTACGGCTTCCCAAAGTTATGTGATTGAAGATTTGTTTAGAAGCTTGATCAAGGAAGTCCACCAAGCAACGAAGGAAGAGGTCCCTGCAGCTGCAGACTTAAATTCCATGAGCTATAGGGAATTGCTACATATACTCGTAACCTACTTGGAGTCTAGAAGGTACCTAGTTGTGTTGGATGACGTGTGGGATATAAAACTTTTGAAAGAAATGAGGATAGCACTTCCAAATAGACAGCTTGGAAGTCGAATCATGCTCACTACTCGAAAAGAAGACCTAGCCTTCTATTCTTTTGGAGTAGAAAGCCATGTTCATCGTATTCAACCGTTGGAAAAGAATGAGGCTTGGGAGCTCTTCAGCAAGAAAGCATTCTCAACTTACCATAAGAAACGTTGTCCACCAGAGCTTGAATCATCAGCTTGGGAACTTTTGGGGAAATGTAAAGGTCTACCCCTAGCAATCGTAGCTTTAGGTGGTCTTACGTCTTCCAAGGAGTCATCTACGGAGTGGAGAAAAGTCTGCAACAGCATAAATTGGCATCTAATAAATGATCATTTCCTAGAACCTTTGAAGACAATCTTGTTTCTCAGTTTCAACGATTTGCCATATCGACTGAAACATTGCTTCCTATATTGTTCAATTTTCCCAGAAGATTATTTGATTCGAGCAGAAAGGCTTATTAGATTGTGGATAGCTGAAGGCTTCGTTGAACATGTTAAAGGGGTCACACTGGAAGAAGTTTCTGAGAGCTATCTTATGGAACTCAATTTTCGTAGCATGTTGCAGGTTGTAAGGTGTCCAACTATAAGACAAGCATGTAAGATGCACGATCTTATGCGGGAGCTTGCTTTGTCCACATTAGAAAAAGAAAAGTTTTGTGTTGTCTATGATGGTAGAGAAGTAATGGAAGAAATCAGAGCTCGTCGTTTGTCAATTCAGACATCTGAAGGAGAAATTAAAGTATGCAAAGGTATGTCGCAGCTTCATTCATTTCATGTGTTTGTCACTGGTGTATTCTGGCCGTCTATTTCAAGTACATTGCTTTCTCAATTCAAATTGTTGAGGATTCTAGATTTGGAGAATGTACCAATTGAAGAACTGCCTGATGGTCTTATGTACTTATTCAACTTAAGATATTTAAGTTTGAGTAGAACTTCAATTAAAAGACTTCCAGAATCCATAGGACAGCTTTGCAATCTTCAAACCTTAGACATCAGCGACACAGAGATTGAAACACTTCCGAAAGAAATTGCCAAGTTGGTAAACCTACGCCATCTAATTATATATGCTAAAGGGATTAGAGCACCATCCAATATTTGTATGTTGAAAACATTGCAAGTTTTGTCATTTGTTGAATCAGACTCGGAAGGAAACTTTTTTAAACTTGTTGGGAACATGACCCAACTTACTCACATTGGCATTACAAATGTAAAAGGAAGTAATGAGATGAACCTTTGTGCTTCCATTCAAAAGATGAAGCTCCTTTGCTACCTGTATTTATTGGTTACAAGAGAAGAGGAATTTCTTCGAATCGATGCATTTGCTTCTCTTCCAGGTCCTCCGCCACACCTTCAAAGGCTTCTTTTGAGCGGCAAACTAGCAACTGTACCAAGTTGGTTTGCTTCACTCAGGAGCCTCACAGATATCAGTCTACGTTGGTCCAGACTTAAAGAAGATGTACTACCACACATTGAAGCACTGCTCTGTCTTAGAAGGCTTATCCTTGTTAATGCATATGTTGGGAATGAGTTGTGCTTCAATATAGGCTTTGCAAGGCTGACACATCTGGAGTTGCTTAATTTCCCCTGCTTGAAAAATATAACAATAGAAGAAGGTGTGATGCCGAAACTCCAGTTGTTAATACTTCATTGCTGCATGAAATTAAAGGCATTACCACATGGTCTTGAATTCCTTAGGAATCTAGAAACATTGAGATTGGGGTCTGTTCCGATGAAAATTATAGAAAATATACGAGAAGGAGGCTTGGATCATCCAAAGGTACAACACATCCACGAAATTGACCAAATTTATGAAACATCATCTGCAACTTTTTCTGCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

900

Amino Acids

102.88

Weight (kDa)

7.31

Isoelectric Point (pI)

51.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 116
Acc36I ACCTGC 1 cut(s) 1411
Acc65I GGTACC 1 cut(s) 798
AccB1I GGYRCC 1 cut(s) 798
AccBSI CCGCTC 1 cut(s) 2226
AccI GTMKAC 2 cut(s) 1084, 2287
AciI CCGC 5 cut(s) 158, 533, 1474, 2198, 2226
AclI AACGTT 1 cut(s) 1027
AclWI GGATC 3 cut(s) 374, 568, 2631
AcoI YGGCCR 1 cut(s) 1655
AcsI RAATTY 4 cut(s) 739, 2156, 2543, 2661
AcuI CTGAAG 3 cut(s) 976, 1353, 1602
AcyI GRCGYC 1 cut(s) 301
AfaI GTAC 7 cut(s) 800, 1672, 1722, 1754, 2244, 2316, 2637
AfiI CCNNNNNNNGG 1 cut(s) 389
AflII CTTAAG 1 cut(s) 1765
AflIII ACRYGT 2 cut(s) 819, 1348
AjiI CACGTC 1 cut(s) 820
AjnI CCWGG 1 cut(s) 2188
Alw21I GWGCWC 3 cut(s) 990, 1558, 1942
Alw26I GTCTC 2 cut(s) 170, 607
AlwI GGATC 3 cut(s) 374, 568, 2631
AoxI GGCC 3 cut(s) 3, 204, 1655
ApeKI GCWGC 5 cut(s) 255, 725, 728, 1615, 2508
ApoI RAATTY 4 cut(s) 739, 2156, 2543, 2661
Asp700I GAANNNNTTC 5 cut(s) 128, 397, 1004, 1872, 2160
Asp718I GGTACC 1 cut(s) 798
AspS9I GGNCC 3 cut(s) 718, 2192, 2295
AsuHPI GGTGA 1 cut(s) 433
AsuII TTCGAA 1 cut(s) 2164
AsuNHI GCTAGC 1 cut(s) 188
AvaII GGWCC 3 cut(s) 718, 2192, 2295
AxyI CCTNAGG 1 cut(s) 2549
BanI GGYRCC 1 cut(s) 798
BanII GRGCYC 2 cut(s) 990, 1558
BarI GAAGNNNNNNTAC 2 cut(s) 2301, 2333
BbsI GAAGAC 7 cut(s) 432, 465, 486, 575, 915, 1113, 1214
Bbv12I GWGCWC 3 cut(s) 990, 1558, 1942
BbvCI CCTCAGC 1 cut(s) 144
BbvI GCAGC 5 cut(s) 242, 715, 737, 1627, 2495
BccI CCATC 7 cut(s) 347, 493, 498, 1520, 1736, 1917, 1951
BceAI ACGGC 2 cut(s) 657, 1642
BcgI CGANNNNNNTGC 2 cut(s) 2159, 2193
BciT130I CCWGG 1 cut(s) 2190
BclI TGATCA 3 cut(s) 85, 684, 1186
BcoDI GTCTC 2 cut(s) 170, 607
BfmI CTRYAG 3 cut(s) 723, 729, 751
BfrI CTTAAG 1 cut(s) 1765
BfuAI ACCTGC 1 cut(s) 1411
BisI GCNGC 6 cut(s) 256, 726, 729, 1616, 2227, 2509
BlsI GCNGC 6 cut(s) 257, 727, 730, 1617, 2228, 2510
Bme1390I CCNGG 1 cut(s) 2190
Bme18I GGWCC 3 cut(s) 718, 2192, 2295
BmgBI CACGTC 1 cut(s) 820
BmgT120I GGNCC 3 cut(s) 718, 2192, 2295
BmiI GGNNCC 3 cut(s) 720, 800, 2270
BmrFI CCNGG 1 cut(s) 2190
BmrI ACTGGG 1 cut(s) 591
BmsI GCATC 7 cut(s) 54, 71, 130, 1183, 1449, 2161, 2466
BmtI GCTAGC 1 cut(s) 192
BmuI ACTGGG 1 cut(s) 591
BpiI GAAGAC 7 cut(s) 432, 465, 486, 575, 915, 1113, 1214
BpmI CTGGAG 2 cut(s) 2444, 2471
Bpu10I CCTNAGC 1 cut(s) 144
Bpu14I TTCGAA 1 cut(s) 2164
BpuEI CTTGAG 1 cut(s) 71
Bsa29I ATCGAT 1 cut(s) 2169
BsaBI GATNNNNATC 1 cut(s) 378
BsaHI GRCGYC 1 cut(s) 301
BsaJI CCNNGG 1 cut(s) 1125
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 4 cut(s) 597, 1371, 1648, 2488
Bse21I CCTNAGG 1 cut(s) 2549
Bse3DI GCAATG 4 cut(s) 1255, 1673, 1967, 2503
Bse8I GATNNNNATC 1 cut(s) 378
BseBI CCWGG 1 cut(s) 2190
BseCI ATCGAT 1 cut(s) 2169
BseDI CCNNGG 1 cut(s) 1125
BseGI GGATG 8 cut(s) 45, 62, 145, 509, 820, 1943, 2626, 2643
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMI GCAATG 4 cut(s) 1255, 1673, 1967, 2503
BseMII CTCAG 4 cut(s) 135, 1237, 1371, 2278
BseNI ACTGG 4 cut(s) 597, 1371, 1648, 2488
BseRI GAGGAG 1 cut(s) 188
BseXI GCAGC 5 cut(s) 242, 715, 737, 1627, 2495
BshFI GGCC 3 cut(s) 5, 206, 1657
BshNI GGYRCC 1 cut(s) 798
BshVI ATCGAT 1 cut(s) 2169
BsiHKAI GWGCWC 3 cut(s) 990, 1558, 1942
BslFI GGGAC 1 cut(s) 704
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 2 cut(s) 170, 607
BsmFI GGGAC 1 cut(s) 704
BsmI GAATGC 1 cut(s) 1004
BsnI GGCC 3 cut(s) 5, 206, 1657
Bsp119I TTCGAA 1 cut(s) 2164
Bsp1286I GDGCHC 3 cut(s) 990, 1558, 1942
Bsp143I GATC 8 cut(s) 19, 85, 379, 560, 684, 1186, 1465, 2623
BspACI CCGC 5 cut(s) 158, 533, 1474, 2198, 2226
BspANI GGCC 3 cut(s) 5, 206, 1657
BspCNI CTCAG 4 cut(s) 136, 1236, 1372, 2277
BspDI ATCGAT 1 cut(s) 2169
BspLI GGNNCC 3 cut(s) 720, 800, 2270
BspMAI CTGCAG 2 cut(s) 727, 733
BspMI ACCTGC 1 cut(s) 1411
BspOI GCTAGC 1 cut(s) 192
BspPI GGATC 3 cut(s) 374, 568, 2631
BspQI GCTCTTC 1 cut(s) 995
BspT104I TTCGAA 1 cut(s) 2164
BspT107I GGYRCC 1 cut(s) 798
BspTI CTTAAG 1 cut(s) 1765
BsrBI CCGCTC 1 cut(s) 2226
BsrDI GCAATG 4 cut(s) 1255, 1673, 1967, 2503
BsrI ACTGG 4 cut(s) 597, 1371, 1648, 2488
BssECI CCNNGG 1 cut(s) 1125
BssMI GATC 8 cut(s) 19, 85, 379, 560, 684, 1186, 1465, 2623
BssNI GRCGYC 1 cut(s) 301
BssT1I CCWWGG 1 cut(s) 1125
Bst2UI CCWGG 1 cut(s) 2190
Bst4CI ACNGT 2 cut(s) 963, 2242
Bst6I CTCTTC 5 cut(s) 605, 708, 995, 2145, 2190
BstACI GRCGYC 1 cut(s) 301
BstAFI CTTAAG 1 cut(s) 1765
BstBI TTCGAA 1 cut(s) 2164
BstC8I GCNNGC 4 cut(s) 43, 190, 253, 1482
BstDEI CTNAG 7 cut(s) 144, 1223, 1380, 1843, 2264, 2345, 2549
BstF5I GGATG 8 cut(s) 45, 62, 145, 509, 820, 1943, 2626, 2643
BstKTI GATC 8 cut(s) 22, 88, 382, 563, 687, 1189, 1468, 2626
BstMAI GTCTC 2 cut(s) 170, 607
BstMBI GATC 8 cut(s) 19, 85, 379, 560, 684, 1186, 1465, 2623
BstMWI GCNNNNNNNGC 2 cut(s) 11, 1100
BstNI CCWGG 1 cut(s) 2190
BstNSI RCATGY 3 cut(s) 1352, 1417, 1455
BstSCI CCNGG 1 cut(s) 2188
BstSFI CTRYAG 3 cut(s) 723, 729, 751
BstV1I GCAGC 5 cut(s) 242, 715, 737, 1627, 2495
BstV2I GAAGAC 7 cut(s) 432, 465, 486, 575, 915, 1113, 1214
Bsu15I ATCGAT 1 cut(s) 2169
Bsu36I CCTNAGG 1 cut(s) 2549
BsuRI GGCC 3 cut(s) 5, 206, 1657
BsuTUI ATCGAT 1 cut(s) 2169
BtrI CACGTC 1 cut(s) 820
BtsCI GGATG 8 cut(s) 45, 62, 145, 509, 820, 1943, 2626, 2643
BtsI GCAGTG 1 cut(s) 2333
BtsIMutI CAGTG 4 cut(s) 604, 1364, 1641, 2333
BveI ACCTGC 1 cut(s) 1411
Cac8I GCNNGC 4 cut(s) 43, 190, 253, 1482
Cfr13I GGNCC 3 cut(s) 718, 2192, 2295
ClaI ATCGAT 1 cut(s) 2169
CseI GACGC 1 cut(s) 290
Csp6I GTAC 7 cut(s) 799, 1671, 1721, 1753, 2243, 2315, 2636
CviQI GTAC 7 cut(s) 799, 1671, 1721, 1753, 2243, 2315, 2636
DdeI CTNAG 7 cut(s) 144, 1223, 1380, 1843, 2264, 2345, 2549
DpnI GATC 8 cut(s) 21, 87, 381, 562, 686, 1188, 1467, 2625
DpnII GATC 8 cut(s) 19, 85, 379, 560, 684, 1186, 1465, 2623
DraI TTTAAA 1 cut(s) 2017
EaeI YGGCCR 1 cut(s) 1655
Eam1104I CTCTTC 5 cut(s) 605, 708, 995, 2145, 2190
EarI CTCTTC 5 cut(s) 605, 708, 995, 2145, 2190
EciI GGCGGA 1 cut(s) 2187
Ecl136II GAGCTC 2 cut(s) 988, 1556
Eco130I CCWWGG 1 cut(s) 1125
Eco24I GRGCYC 2 cut(s) 990, 1558
Eco32I GATATC 2 cut(s) 223, 2281
Eco47I GGWCC 3 cut(s) 718, 2192, 2295
Eco53kI GAGCTC 2 cut(s) 988, 1556
Eco57I CTGAAG 3 cut(s) 976, 1353, 1602
Eco81I CCTNAGG 1 cut(s) 2549
EcoICRI GAGCTC 2 cut(s) 988, 1556
EcoO109I RGGNCCY 2 cut(s) 718, 2192
EcoRI GAATTC 1 cut(s) 2543
EcoRII CCWGG 1 cut(s) 2188
EcoRV GATATC 2 cut(s) 223, 2281
EcoT14I CCWWGG 1 cut(s) 1125
EcoT22I ATGCAT 2 cut(s) 2176, 2371
EcoT38I GRGCYC 2 cut(s) 990, 1558
ErhI CCWWGG 1 cut(s) 1125
FalI AAGNNNNNCTT 4 cut(s) 450, 482, 1199, 1231
FaqI GGGAC 1 cut(s) 704
FauI CCCGC 1 cut(s) 1467
FauNDI CATATG 1 cut(s) 2371
FbaI TGATCA 3 cut(s) 85, 684, 1186
FblI GTMKAC 2 cut(s) 1084, 2287
Fnu4HI GCNGC 6 cut(s) 256, 726, 729, 1616, 2227, 2509
FokI GGATG 8 cut(s) 32, 49, 152, 516, 827, 1930, 2613, 2630
FriOI GRGCYC 2 cut(s) 990, 1558
Fsp4HI GCNGC 6 cut(s) 256, 726, 729, 1616, 2227, 2509
GluI GCNGC 6 cut(s) 256, 726, 729, 1616, 2227, 2509
GsuI CTGGAG 2 cut(s) 2444, 2471
HaeIII GGCC 3 cut(s) 5, 206, 1657
HgaI GACGC 1 cut(s) 290
Hin1I GRCGYC 1 cut(s) 301
HindIII AAGCTT 1 cut(s) 679
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 6 cut(s) 697, 1034, 1085, 1491, 1900, 2288
Hpy8I GTNNAC 6 cut(s) 697, 1034, 1085, 1491, 1900, 2288
Hpy99I CGWCG 1 cut(s) 1563
HpyCH4III ACNGT 2 cut(s) 963, 2242
HpyCH4IV ACGT 4 cut(s) 819, 1027, 1118, 2290
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 1100
HpyF3I CTNAG 7 cut(s) 144, 1223, 1380, 1843, 2264, 2345, 2549
HpySE526I ACGT 4 cut(s) 819, 1027, 1118, 2290
Hsp92I GRCGYC 1 cut(s) 301
KpnI GGTACC 1 cut(s) 802
Ksp22I TGATCA 3 cut(s) 85, 684, 1186
Kzo9I GATC 8 cut(s) 19, 85, 379, 560, 684, 1186, 1465, 2623
LguI GCTCTTC 1 cut(s) 995
LmnI GCTCC 4 cut(s) 985, 1477, 2121, 2268
Lsp1109I GCAGC 5 cut(s) 242, 715, 737, 1627, 2495
LweI GCATC 7 cut(s) 54, 71, 130, 1183, 1449, 2161, 2466
MaeII ACGT 4 cut(s) 819, 1027, 1118, 2290
MaeIII GTNAC 5 cut(s) 318, 775, 1360, 1639, 2140
MalI GATC 8 cut(s) 21, 87, 381, 562, 686, 1188, 1467, 2625
MbiI CCGCTC 1 cut(s) 2226
MboI GATC 8 cut(s) 19, 85, 379, 560, 684, 1186, 1465, 2623
MfeI CAATTG 1 cut(s) 1725
MhlI GDGCHC 3 cut(s) 990, 1558, 1942
MlyI GAGTC 4 cut(s) 446, 797, 1139, 1991
MmeI TCCRAC 4 cut(s) 529, 792, 945, 1460
Mph1103I ATGCAT 2 cut(s) 2176, 2371
MroXI GAANNNNTTC 5 cut(s) 128, 397, 1004, 1872, 2160
MspA1I CMGCKG 2 cut(s) 624, 728
MspCI CTTAAG 1 cut(s) 1765
MspR9I CCNGG 1 cut(s) 2190
MunI CAATTG 1 cut(s) 1725
Mva1269I GAATGC 1 cut(s) 1004
MvaI CCWGG 1 cut(s) 2190
MwoI GCNNNNNNNGC 2 cut(s) 11, 1100
NdeI CATATG 1 cut(s) 2371
NdeII GATC 8 cut(s) 19, 85, 379, 560, 684, 1186, 1465, 2623
NheI GCTAGC 1 cut(s) 188
NlaIV GGNNCC 3 cut(s) 720, 800, 2270
NmuCI GTSAC 2 cut(s) 1360, 1639
NsiI ATGCAT 2 cut(s) 2176, 2371
NspI RCATGY 3 cut(s) 1352, 1417, 1455
NspV TTCGAA 1 cut(s) 2164
PciI ACATGT 1 cut(s) 1348
PciSI GCTCTTC 1 cut(s) 995
PctI GAATGC 1 cut(s) 1004
PdmI GAANNNNTTC 5 cut(s) 128, 397, 1004, 1872, 2160
PfeI GAWTC 9 cut(s) 361, 885, 1046, 1297, 1702, 1811, 1991, 2166, 2554
PkrI GCNGC 6 cut(s) 257, 727, 730, 1617, 2228, 2510
PleI GAGTC 4 cut(s) 445, 796, 1138, 1991
PpsI GAGTC 4 cut(s) 445, 796, 1138, 1991
PpuMI RGGWCCY 2 cut(s) 718, 2192
PscI ACATGT 1 cut(s) 1348
PsiI TTATAA 1 cut(s) 116
Psp124BI GAGCTC 2 cut(s) 990, 1558
Psp1406I AACGTT 1 cut(s) 1027
Psp5II RGGWCCY 2 cut(s) 718, 2192
Psp6I CCWGG 1 cut(s) 2188
PspGI CCWGG 1 cut(s) 2188
PspN4I GGNNCC 3 cut(s) 720, 800, 2270
PspPI GGNCC 3 cut(s) 718, 2192, 2295
PspPPI RGGWCCY 2 cut(s) 718, 2192
PstI CTGCAG 2 cut(s) 727, 733
PvuII CAGCTG 2 cut(s) 624, 728
RsaI GTAC 7 cut(s) 800, 1672, 1722, 1754, 2244, 2316, 2637
RsaNI GTAC 7 cut(s) 799, 1671, 1721, 1753, 2243, 2315, 2636
SacI GAGCTC 2 cut(s) 990, 1558
SapI GCTCTTC 1 cut(s) 995
SatI GCNGC 6 cut(s) 256, 726, 729, 1616, 2227, 2509
Sau3AI GATC 8 cut(s) 19, 85, 379, 560, 684, 1186, 1465, 2623
Sau96I GGNCC 3 cut(s) 718, 2192, 2295
SchI GAGTC 4 cut(s) 446, 797, 1139, 1991
ScrFI CCNGG 1 cut(s) 2190
SduI GDGCHC 3 cut(s) 990, 1558, 1942
SfaNI GCATC 7 cut(s) 54, 71, 130, 1183, 1449, 2161, 2466
SfcI CTRYAG 3 cut(s) 723, 729, 751
SfuI TTCGAA 1 cut(s) 2164
SinI GGWCC 3 cut(s) 718, 2192, 2295
SmlI CTYRAG 2 cut(s) 50, 1765
SmoI CTYRAG 2 cut(s) 50, 1765
SsiI CCGC 5 cut(s) 158, 533, 1474, 2198, 2226
SspI AATATT 2 cut(s) 495, 1951
SstI GAGCTC 2 cut(s) 990, 1558
StyD4I CCNGG 1 cut(s) 2188
StyI CCWWGG 1 cut(s) 1125
TaaI ACNGT 2 cut(s) 963, 2242
TaiI ACGT 4 cut(s) 822, 1030, 1121, 2293
TaqI TCGA 7 cut(s) 244, 883, 901, 1246, 1300, 2164, 2169
TatI WGTACW 3 cut(s) 1670, 1752, 2314
TauI GCSGC 1 cut(s) 2229
TfiI GAWTC 9 cut(s) 361, 885, 1046, 1297, 1702, 1811, 1991, 2166, 2554
TscAI CASTG 4 cut(s) 604, 1371, 1648, 2340
TseFI GTSAC 2 cut(s) 1360, 1639
TseI GCWGC 5 cut(s) 255, 725, 728, 1615, 2508
Tsp45I GTSAC 2 cut(s) 1360, 1639
TspGWI ACGGA 2 cut(s) 531, 1154
TspRI CASTG 4 cut(s) 604, 1371, 1648, 2340
Vha464I CTTAAG 1 cut(s) 1765
VpaK11BI GGWCC 3 cut(s) 718, 2192, 2295
XapI RAATTY 4 cut(s) 739, 2156, 2543, 2661
XbaI TCTAGA 3 cut(s) 791, 1705, 2557
XceI RCATGY 3 cut(s) 1352, 1417, 1455
XcmI CCANNNNNNNNNTGG 1 cut(s) 288
XmiI GTMKAC 2 cut(s) 1084, 2287
XmnI GAANNNNTTC 5 cut(s) 128, 397, 1004, 1872, 2160
Zsp2I ATGCAT 2 cut(s) 2176, 2371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.