Prupe.6G146200_v2.0.a1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000110
Physical Location & Seq
Reverse (-)
12096690 .. 12099324
2635 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G146200.1

Sequence Viewer

Length: 2589 bp
ATGACGTTAGTTGCAGCTGACCTCTTCATTGACAAATTTGTAAGCATTCTTGAGAGCGAAGCAGTATCCATAGCCGGCGTTCGTGATCAAGTTGATGAGATTAAGCGGGAGCTGGTGATCATGAAATCCTTCTTAGAGGATGATGACGGAGGCAAGAAAGCACACACAGAAGTACAGAAAGCGTGCGTCGCAAGTATTCGAGACTTGGCCAATGATGTCGAAAACATCATTGATGAATTCATGCATAACAAGTACAAGCAACAAAGGGGCGGTCCATGTCCAAAGTGGCTCCTCAAAACCATTCACTTTCCAAAGAACCTTTGGTATATGGGTCAAATAGCCAAGAAGTTAAAGAAAATTGCCGGAAGGATTAGAGCTATTCCAGAGAGAAATAAGAGATATGGTGGTGCTGTAGCCGTAGAAGGAAAATGTACTTCTGAGGATATTCGCAGATGGGTGCAGAACCAAGCGGAGTCTTCTCTTTACCATAAGGAGAATGAACTCGTCGGGATTGAAGACGATAAGAAAATGTTGATGGGATGGGTGATGAACAACGAGCAACACCAAATTGTTGTCTCCGTGGTAGGGATGGGAGGATCAGGGAAGACAACTCTAGTTGCAAGGACTTTCACCAACGAGGTAGTCAAGAAACATTTCGAATGTTACGCCTGGATTACTGTTTCCCAATCTTACGTGATTGAAGACTTACTTAGAAGATTGATCAAAGAATTCCACAAAGCAAGGAGGGAAGAGGTCTCTGCAGACATGAATGCCATGTGCTATATCGAATTGGTTGAGATTTTGGTTAACTACTTGGAGACTAAACGGTACCTTGTTGTATTGGATGACGTGTGGGATATCCTTCTTTGGGAAAGAATAAGGCTTTCATTTCCAGATAAACAACTTGGAAGTCGAGTCATGCTTACAACTCGAAGAGAGGACATAGATTCAACCGCTGGAAAGGAGAGATGCTTCTTCAGCATGAAAGCCTTCTCAAGTTATCATAACAAATCTTGTTCACCGGAACTTCTGCCATTAGCTCGGGAACTTGTGGAAAAGTGTGAAGGTCTGCCTCTTGCAATTGTAGCCTTAAGTGGTCTCATGTCTTCTAAGAAGTCACTTACCGATTGGAGCGAAGTCTACAATAGCTTAAATTGGCATCTAACAAACAATTCTTTGTTAGAACCTATGAAGAGCATCTTGTTGCTTAGTTTCAATGATTTGCCATACCGATTAAAGCAGTGCTTTCTATATTGTTCCCTTTTTCCAGAAGATGAGGTGATCATAAATCAGAGGCTCATTAAATTGTGGATCGCCGAAGGATTTGTTGAACATGTCAAGGGGGTAACACCAGAAGAAGTTGCAAACAGCTATGTTATGGAACTCATTTTTCGTAACATGCTACAGGAAAGGTATCGAGAATGCCAACAAGCATGTAAGATGCACGATCTTATGCGGGAGATTGCTATGTCTATAGCAGAAAAAGAAAAGTTTTGTGTCGTTCTTGATGGGAGTGAAACAATGGAAGAAACTGGGGCCCTCCGTTTGTCAATTCAAACAACTAATGGAGAAATTGGATCACGCAAAGGTATGTCACGGCTTCGTTCTTTTCTTGTCTTTGCCACTGGCGTATCCTCATTCTCTTTCTCAAAGAAATTTCCTTTTGACTCTCCATTGTTGAGGGTTCTAGATTTGGAGGGTGTCCCAATTGATCGTCTGCCAGATGAACTAACGTACTTGTTTAATTTAAAATATTTGAATTTGAGAAAAACTCGAATTAAGGAGCTTCCAGAATCCATAAGACGGCTCAGCAAGCTTCAGACCTTGGACCTCACAATAGAGGCACTTCCAGTAGGGATTTCCAAGTTGCTCAACCTGCGCCATCTTGTTATGTGGCATTCCAAAAGCGGTTCAGAGGTAGTTGGGGTAAAATTACCATCAAGTATTAGTAAGATGAAGAAATTGCAATCTTTAGGATGTATTGAATTAGAAGGAAATATTATTAGACTTATTGGAAGTATGACCCAGCTTAAGGTCCTTGGCATTACAAATGTGAAAGAAAGAGACGAGAAGGACCTATGTGCCTCAATTCAAGAGATGAAGGTCCTTTCCCATTTGCATTTATTGGTAGCAGATGGAGAGGAATTTCTTCGAGTTGATGCATTGTCTTCACCGTCATCCTACCTTGATAGACTTTACTTGATAGGCAAACTAGAAAAAGTACCACATTGGTTTTGTTCACTCCAGTGTCTTACATCTTTGCATCTGCACGGGTCCAGACTTGAAGAAGATTTACGACCTCATATTGAAGCATTGCCCTCTCTACTTTCGCTTTGCCTTAATGATTCCTATGTTGGGAAAGAGTTGTGTTTCAGTAGAGGCTTTGTGAAGCTTAGGTATTTGGAGTTGAGGAATCTCTCATTATTAAACAAGATGACCATAGAAAAACAGGTGATGCCAAATCTGGAGTTCTTAGGCATCTATAGGTTCTTGGGATTAGAGACATTGCCACAGGGTATTGAGCACCTTACTAAACTACAAGGATACACATTTGAATGTGTTTCAGAGAAATTTGTAGAGTCCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

863

Amino Acids

98.79

Weight (kDa)

7.5

Isoelectric Point (pI)

51.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1884
Acc65I GGTACC 1 cut(s) 828
AccB1I GGYRCC 1 cut(s) 828
AccI GTMKAC 1 cut(s) 1140
AciI CCGC 6 cut(s) 106, 270, 470, 954, 1456, 1908
AclWI GGATC 3 cut(s) 604, 1319, 1585
AcoI YGGCCR 1 cut(s) 207
AcsI RAATTY 7 cut(s) 35, 236, 728, 1655, 1759, 2144, 2570
AcuI CTGAAG 2 cut(s) 961, 1802
AfaI GTAC 6 cut(s) 174, 254, 433, 830, 1736, 2223
AfiI CCNNNNNNNGG 5 cut(s) 585, 868, 1803, 2032, 2355
AflII CTTAAG 2 cut(s) 1090, 2030
AflIII ACRYGT 2 cut(s) 849, 1333
AjiI CACGTC 1 cut(s) 850
AjnI CCWGG 1 cut(s) 668
AleI CACNNNNGTG 1 cut(s) 2245
Alw21I GWGCWC 1 cut(s) 2526
Alw26I GTCTC 7 cut(s) 195, 580, 760, 812, 1103, 2058, 2495
AlwI GGATC 3 cut(s) 604, 1319, 1585
Ama87I CYCGRG 1 cut(s) 1041
AoxI GGCC 2 cut(s) 207, 1536
ApaI GGGCCC 1 cut(s) 1540
ApeKI GCWGC 1 cut(s) 14
ApoI RAATTY 7 cut(s) 35, 236, 728, 1655, 1759, 2144, 2570
ArsI GACNNNNNNTTYG 2 cut(s) 256, 288
Asp700I GAANNNNTTC 4 cut(s) 128, 653, 989, 2148
Asp718I GGTACC 1 cut(s) 828
AspLEI GCGC 1 cut(s) 1881
AspS9I GGNCC 8 cut(s) 272, 1536, 1537, 1828, 2035, 2074, 2104, 2274
AsuHPI GGTGA 7 cut(s) 127, 556, 622, 1011, 1291, 2163, 2464
AsuII TTCGAA 1 cut(s) 657
AvaI CYCGRG 1 cut(s) 1041
AvaII GGWCC 6 cut(s) 272, 1828, 2035, 2074, 2104, 2274
BaeGI GKGCMC 1 cut(s) 1540
BaeI ACNNNNGTAYC 2 cut(s) 1614, 1647
BalI TGGCCA 1 cut(s) 209
BanI GGYRCC 1 cut(s) 828
BanII GRGCYC 1 cut(s) 1540
BarI GAAGNNNNNNTAC 2 cut(s) 2277, 2309
BbsI GAAGAC 6 cut(s) 468, 522, 611, 708, 1098, 2160
Bbv12I GWGCWC 1 cut(s) 2526
BbvI GCAGC 1 cut(s) 26
BccI CCATC 8 cut(s) 447, 529, 534, 583, 1502, 1890, 1945, 2129
BceAI ACGGC 3 cut(s) 401, 1613, 1820
BciT130I CCWGG 1 cut(s) 670
BciVI GTATCC 3 cut(s) 76, 1642, 2537
BclI TGATCA 4 cut(s) 85, 117, 720, 1281
BcoDI GTCTC 7 cut(s) 195, 580, 760, 812, 1103, 2058, 2495
BfaI CTAG 3 cut(s) 614, 1688, 2213
BfmI CTRYAG 5 cut(s) 411, 759, 1403, 1473, 2482
BfrI CTTAAG 2 cut(s) 1090, 2030
BfuAI ACCTGC 1 cut(s) 1884
BfuI GTATCC 3 cut(s) 76, 1642, 2537
BisI GCNGC 1 cut(s) 15
BlpI GCTNAGC 1 cut(s) 1808
BlsI GCNGC 1 cut(s) 16
Bme1390I CCNGG 1 cut(s) 670
Bme18I GGWCC 6 cut(s) 272, 1828, 2035, 2074, 2104, 2274
BmeT110I CYCGRG 1 cut(s) 1041
BmgBI CACGTC 1 cut(s) 850
BmgT120I GGNCC 8 cut(s) 272, 1536, 1537, 1828, 2035, 2074, 2104, 2274
BmiI GGNNCC 5 cut(s) 290, 830, 1537, 1538, 2275
BmrFI CCNGG 1 cut(s) 670
BmrI ACTGGG 1 cut(s) 1542
BmsI GCATC 8 cut(s) 959, 1168, 1206, 1431, 2149, 2272, 2445, 2487
BmuI ACTGGG 1 cut(s) 1542
BpiI GAAGAC 6 cut(s) 468, 522, 611, 708, 1098, 2160
BplI GAGNNNNNCTC 2 cut(s) 1756, 1788
BpmI CTGGAG 2 cut(s) 2228, 2486
Bpu10I CCTNAGC 1 cut(s) 2393
Bpu1102I GCTNAGC 1 cut(s) 1808
Bpu14I TTCGAA 1 cut(s) 657
BpuEI CTTGAG 2 cut(s) 71, 979
BsaAI YACGTR 1 cut(s) 694
BsaI GGTCTC 2 cut(s) 760, 1103
BsaJI CCNNGG 3 cut(s) 579, 1824, 2038
BsaWI WCCGGW 1 cut(s) 1021
Bsc4I CCNNNNNNNGG 5 cut(s) 585, 868, 1803, 2032, 2355
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 4 cut(s) 1537, 1630, 1850, 2245
Bse3DI GCAATG 2 cut(s) 2312, 2504
BseBI CCWGG 1 cut(s) 670
BseDI CCNNGG 3 cut(s) 579, 1824, 2038
BseGI GGATG 6 cut(s) 145, 545, 594, 850, 1982, 2177
BseLI CCNNNNNNNGG 5 cut(s) 585, 868, 1803, 2032, 2355
BseMI GCAATG 2 cut(s) 2312, 2504
BseMII CTCAG 2 cut(s) 429, 1822
BseNI ACTGG 4 cut(s) 1537, 1630, 1850, 2245
BseRI GAGGAG 1 cut(s) 281
BseSI GKGCMC 1 cut(s) 1540
BseXI GCAGC 1 cut(s) 26
BseYI CCCAGC 1 cut(s) 2025
BsgI GTGCAG 2 cut(s) 479, 2252
BshFI GGCC 2 cut(s) 209, 1538
BshNI GGYRCC 1 cut(s) 828
BsiHKAI GWGCWC 1 cut(s) 2526
BsiHKCI CYCGRG 1 cut(s) 1041
BsiSI CCGG 3 cut(s) 75, 363, 1022
BslFI GGGAC 1 cut(s) 1688
BslI CCNNNNNNNGG 5 cut(s) 585, 868, 1803, 2032, 2355
BsmAI GTCTC 7 cut(s) 195, 580, 760, 812, 1103, 2058, 2495
BsmBI CGTCTC 1 cut(s) 2058
BsmFI GGGAC 1 cut(s) 1688
BsmI GAATGC 4 cut(s) 45, 775, 1427, 1897
BsnI GGCC 2 cut(s) 209, 1538
Bso31I GGTCTC 2 cut(s) 760, 1103
BsoBI CYCGRG 1 cut(s) 1041
Bsp119I TTCGAA 1 cut(s) 657
Bsp120I GGGCCC 1 cut(s) 1536
Bsp1286I GDGCHC 2 cut(s) 1540, 2526
Bsp143I GATC 9 cut(s) 85, 117, 596, 720, 1281, 1311, 1447, 1577, 1711
Bsp1720I GCTNAGC 1 cut(s) 1808
BspACI CCGC 6 cut(s) 106, 270, 470, 954, 1456, 1908
BspANI GGCC 2 cut(s) 209, 1538
BspCNI CTCAG 2 cut(s) 430, 1821
BspHI TCATGA 1 cut(s) 120
BspLI GGNNCC 5 cut(s) 290, 830, 1537, 1538, 2275
BspMAI CTGCAG 1 cut(s) 763
BspMI ACCTGC 1 cut(s) 1884
BspPI GGATC 3 cut(s) 604, 1319, 1585
BspQI GCTCTTC 1 cut(s) 1187
BspT104I TTCGAA 1 cut(s) 657
BspT107I GGYRCC 1 cut(s) 828
BspTI CTTAAG 2 cut(s) 1090, 2030
BspTNI GGTCTC 2 cut(s) 760, 1103
BsrDI GCAATG 2 cut(s) 2312, 2504
BsrFI RCCGGY 1 cut(s) 74
BsrI ACTGG 4 cut(s) 1537, 1630, 1850, 2245
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 3 cut(s) 579, 1824, 2038
BssMI GATC 9 cut(s) 85, 117, 596, 720, 1281, 1311, 1447, 1577, 1711
BssT1I CCWWGG 2 cut(s) 1824, 2038
Bst2UI CCWGG 1 cut(s) 670
Bst4CI ACNGT 3 cut(s) 679, 828, 2175
Bst6I CTCTTC 4 cut(s) 29, 744, 928, 1187
BstAFI CTTAAG 2 cut(s) 1090, 2030
BstBAI YACGTR 1 cut(s) 694
BstBI TTCGAA 1 cut(s) 657
BstC8I GCNNGC 3 cut(s) 76, 184, 1814
BstDEI CTNAG 8 cut(s) 133, 438, 710, 1110, 1208, 1808, 2393, 2473
BstDSI CCRYGG 1 cut(s) 579
BstF5I GGATG 6 cut(s) 145, 545, 594, 850, 1982, 2177
BstHHI GCGC 1 cut(s) 1881
BstKTI GATC 9 cut(s) 88, 120, 599, 723, 1284, 1314, 1450, 1580, 1714
BstMAI GTCTC 7 cut(s) 195, 580, 760, 812, 1103, 2058, 2495
BstMBI GATC 9 cut(s) 85, 117, 596, 720, 1281, 1311, 1447, 1577, 1711
BstMWI GCNNNNNNNGC 5 cut(s) 188, 978, 1085, 1813, 1876
BstNI CCWGG 1 cut(s) 670
BstNSI RCATGY 3 cut(s) 1337, 1402, 1437
BstSCI CCNGG 1 cut(s) 668
BstSFI CTRYAG 5 cut(s) 411, 759, 1403, 1473, 2482
BstSLI GKGCMC 1 cut(s) 1540
BstV1I GCAGC 1 cut(s) 26
BstV2I GAAGAC 6 cut(s) 468, 522, 611, 708, 1098, 2160
BsuI GTATCC 3 cut(s) 76, 1642, 2537
BsuRI GGCC 2 cut(s) 209, 1538
BtgI CCRYGG 1 cut(s) 579
BtrI CACGTC 1 cut(s) 850
BtsCI GGATG 6 cut(s) 145, 545, 594, 850, 1982, 2177
BtsI GCAGTG 1 cut(s) 1247
BtsIMutI CAGTG 3 cut(s) 1247, 1623, 2252
BveI ACCTGC 1 cut(s) 1884
Cac8I GCNNGC 3 cut(s) 76, 184, 1814
CciI TCATGA 1 cut(s) 120
CfoI GCGC 1 cut(s) 1881
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 8 cut(s) 272, 1536, 1537, 1828, 2035, 2074, 2104, 2274
CseI GACGC 1 cut(s) 175
Csp6I GTAC 6 cut(s) 173, 253, 432, 829, 1735, 2222
CviQI GTAC 6 cut(s) 173, 253, 432, 829, 1735, 2222
DdeI CTNAG 8 cut(s) 133, 438, 710, 1110, 1208, 1808, 2393, 2473
DpnI GATC 9 cut(s) 87, 119, 598, 722, 1283, 1313, 1449, 1579, 1713
DpnII GATC 9 cut(s) 85, 117, 596, 720, 1281, 1311, 1447, 1577, 1711
DraI TTTAAA 1 cut(s) 1749
EaeI YGGCCR 1 cut(s) 207
Eam1104I CTCTTC 4 cut(s) 29, 744, 928, 1187
EarI CTCTTC 4 cut(s) 29, 744, 928, 1187
Eco130I CCWWGG 2 cut(s) 1824, 2038
Eco24I GRGCYC 1 cut(s) 1540
Eco31I GGTCTC 2 cut(s) 760, 1103
Eco32I GATATC 1 cut(s) 859
Eco47I GGWCC 6 cut(s) 272, 1828, 2035, 2074, 2104, 2274
Eco57I CTGAAG 2 cut(s) 961, 1802
Eco88I CYCGRG 1 cut(s) 1041
EcoO109I RGGNCCY 5 cut(s) 1536, 1537, 2035, 2074, 2104
EcoRI GAATTC 2 cut(s) 236, 728
EcoRII CCWGG 1 cut(s) 668
EcoRV GATATC 1 cut(s) 859
EcoT14I CCWWGG 2 cut(s) 1824, 2038
EcoT22I ATGCAT 2 cut(s) 246, 2164
EcoT38I GRGCYC 1 cut(s) 1540
ErhI CCWWGG 2 cut(s) 1824, 2038
Esp3I CGTCTC 1 cut(s) 2058
FalI AAGNNNNNCTT 6 cut(s) 693, 725, 1184, 1216, 1229, 1261
FaqI GGGAC 1 cut(s) 1688
FauI CCCGC 2 cut(s) 99, 1449
FauNDI CATATG 1 cut(s) 2585
FbaI TGATCA 4 cut(s) 85, 117, 720, 1281
FblI GTMKAC 1 cut(s) 1140
Fnu4HI GCNGC 1 cut(s) 15
FokI GGATG 6 cut(s) 152, 552, 601, 857, 1989, 2164
FriOI GRGCYC 1 cut(s) 1540
Fsp4HI GCNGC 1 cut(s) 15
FspBI CTAG 3 cut(s) 614, 1688, 2213
GlaI GCGC 1 cut(s) 1880
GluI GCNGC 1 cut(s) 15
GsaI CCCAGC 1 cut(s) 2029
GsuI CTGGAG 2 cut(s) 2228, 2486
HaeIII GGCC 2 cut(s) 209, 1538
HapII CCGG 3 cut(s) 75, 363, 1022
HgaI GACGC 1 cut(s) 175
HhaI GCGC 1 cut(s) 1881
Hin6I GCGC 1 cut(s) 1879
HinP1I GCGC 1 cut(s) 1879
HincII GTYRAC 1 cut(s) 808
HindII GTYRAC 1 cut(s) 808
HindIII AAGCTT 2 cut(s) 1814, 2390
HinfI GANTC 8 cut(s) 473, 915, 947, 1667, 1793, 2345, 2413, 2579
HpaI GTTAAC 1 cut(s) 808
HpaII CCGG 3 cut(s) 75, 363, 1022
HphI GGTGA 7 cut(s) 127, 556, 622, 1011, 1291, 2163, 2464
Hpy166II GTNNAC 4 cut(s) 808, 1019, 1141, 2240
Hpy188I TCNGA 5 cut(s) 439, 1293, 1821, 1915, 2566
Hpy8I GTNNAC 4 cut(s) 808, 1019, 1141, 2240
Hpy99I CGWCG 2 cut(s) 191, 509
HpyCH4III ACNGT 3 cut(s) 679, 828, 2175
HpyCH4IV ACGT 4 cut(s) 5, 693, 849, 1733
HpyF10VI GCNNNNNNNGC 5 cut(s) 188, 978, 1085, 1813, 1876
HpyF3I CTNAG 8 cut(s) 133, 438, 710, 1110, 1208, 1808, 2393, 2473
HpySE526I ACGT 4 cut(s) 5, 693, 849, 1733
HspAI GCGC 1 cut(s) 1879
KpnI GGTACC 1 cut(s) 832
KroI GCCGGC 1 cut(s) 74
KroNI GCCGGC 1 cut(s) 76
Ksp22I TGATCA 4 cut(s) 85, 117, 720, 1281
KspAI GTTAAC 1 cut(s) 808
Kzo9I GATC 9 cut(s) 85, 117, 596, 720, 1281, 1311, 1447, 1577, 1711
LguI GCTCTTC 1 cut(s) 1187
LmnI GCTCC 4 cut(s) 109, 294, 1131, 1783
Lsp1109I GCAGC 1 cut(s) 26
LweI GCATC 8 cut(s) 959, 1168, 1206, 1431, 2149, 2272, 2445, 2487
MaeI CTAG 3 cut(s) 614, 1688, 2213
MaeII ACGT 4 cut(s) 5, 693, 849, 1733
MaeIII GTNAC 5 cut(s) 662, 1116, 1345, 1394, 1593
MalI GATC 9 cut(s) 87, 119, 598, 722, 1283, 1313, 1449, 1579, 1713
MboI GATC 9 cut(s) 85, 117, 596, 720, 1281, 1311, 1447, 1577, 1711
MfeI CAATTG 2 cut(s) 1080, 1707
MhlI GDGCHC 2 cut(s) 1540, 2526
MlsI TGGCCA 1 cut(s) 209
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 4 cut(s) 482, 924, 1661, 2588
Mox20I TGGCCA 1 cut(s) 209
Mph1103I ATGCAT 2 cut(s) 246, 2164
MroNI GCCGGC 1 cut(s) 74
MroXI GAANNNNTTC 4 cut(s) 128, 653, 989, 2148
MscI TGGCCA 1 cut(s) 209
MslI CAYNNNNRTG 2 cut(s) 2245, 2554
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 2 cut(s) 17, 956
MspCI CTTAAG 2 cut(s) 1090, 2030
MspI CCGG 3 cut(s) 75, 363, 1022
MspR9I CCNGG 1 cut(s) 670
MunI CAATTG 2 cut(s) 1080, 1707
Mva1269I GAATGC 4 cut(s) 45, 775, 1427, 1897
MvaI CCWGG 1 cut(s) 670
MwoI GCNNNNNNNGC 5 cut(s) 188, 978, 1085, 1813, 1876
NaeI GCCGGC 1 cut(s) 76
NdeI CATATG 1 cut(s) 2585
NdeII GATC 9 cut(s) 85, 117, 596, 720, 1281, 1311, 1447, 1577, 1711
NgoMIV GCCGGC 1 cut(s) 74
NlaIV GGNNCC 5 cut(s) 290, 830, 1537, 1538, 2275
NmuCI GTSAC 2 cut(s) 1116, 1593
NsiI ATGCAT 2 cut(s) 246, 2164
NspI RCATGY 3 cut(s) 1337, 1402, 1437
NspV TTCGAA 1 cut(s) 657
OliI CACNNNNGTG 1 cut(s) 2245
PagI TCATGA 1 cut(s) 120
PciI ACATGT 1 cut(s) 1333
PciSI GCTCTTC 1 cut(s) 1187
PctI GAATGC 4 cut(s) 45, 775, 1427, 1897
PdiI GCCGGC 1 cut(s) 76
PdmI GAANNNNTTC 4 cut(s) 128, 653, 989, 2148
PfeI GAWTC 4 cut(s) 947, 1793, 2345, 2413
PkrI GCNGC 1 cut(s) 16
PleI GAGTC 4 cut(s) 481, 923, 1661, 2587
PpsI GAGTC 4 cut(s) 481, 923, 1661, 2587
Ppu21I YACGTR 1 cut(s) 694
PpuMI RGGWCCY 3 cut(s) 2035, 2074, 2104
PscI ACATGT 1 cut(s) 1333
Psp5II RGGWCCY 3 cut(s) 2035, 2074, 2104
Psp6I CCWGG 1 cut(s) 668
PspFI CCCAGC 1 cut(s) 2025
PspGI CCWGG 1 cut(s) 668
PspN4I GGNNCC 5 cut(s) 290, 830, 1537, 1538, 2275
PspOMI GGGCCC 1 cut(s) 1536
PspPI GGNCC 8 cut(s) 272, 1536, 1537, 1828, 2035, 2074, 2104, 2274
PspPPI RGGWCCY 3 cut(s) 2035, 2074, 2104
PstI CTGCAG 1 cut(s) 763
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 6 cut(s) 174, 254, 433, 830, 1736, 2223
RsaNI GTAC 6 cut(s) 173, 253, 432, 829, 1735, 2222
RseI CAYNNNNRTG 2 cut(s) 2245, 2554
SapI GCTCTTC 1 cut(s) 1187
SatI GCNGC 1 cut(s) 15
Sau3AI GATC 9 cut(s) 85, 117, 596, 720, 1281, 1311, 1447, 1577, 1711
Sau96I GGNCC 8 cut(s) 272, 1536, 1537, 1828, 2035, 2074, 2104, 2274
SchI GAGTC 4 cut(s) 482, 924, 1661, 2588
ScrFI CCNGG 1 cut(s) 670
SduI GDGCHC 2 cut(s) 1540, 2526
SfaNI GCATC 8 cut(s) 959, 1168, 1206, 1431, 2149, 2272, 2445, 2487
SfcI CTRYAG 5 cut(s) 411, 759, 1403, 1473, 2482
SfuI TTCGAA 1 cut(s) 657
SinI GGWCC 6 cut(s) 272, 1828, 2035, 2074, 2104, 2274
SmiMI CAYNNNNRTG 2 cut(s) 2245, 2554
SmlI CTYRAG 4 cut(s) 50, 994, 1090, 2030
SmoI CTYRAG 4 cut(s) 50, 994, 1090, 2030
SsiI CCGC 6 cut(s) 106, 270, 470, 954, 1456, 1908
SspI AATATT 2 cut(s) 1754, 1999
SspMI CTAG 3 cut(s) 614, 1688, 2213
StyD4I CCNGG 1 cut(s) 668
StyI CCWWGG 2 cut(s) 1824, 2038
TaaI ACNGT 3 cut(s) 679, 828, 2175
TaiI ACGT 4 cut(s) 8, 696, 852, 1736
TaqI TCGA 9 cut(s) 199, 219, 657, 786, 913, 931, 1417, 1774, 2152
TatI WGTACW 3 cut(s) 172, 252, 431
TfiI GAWTC 4 cut(s) 947, 1793, 2345, 2413
TscAI CASTG 3 cut(s) 1247, 1630, 2252
TseFI GTSAC 2 cut(s) 1116, 1593
TseI GCWGC 1 cut(s) 14
Tsp45I GTSAC 2 cut(s) 1116, 1593
TspGWI ACGGA 3 cut(s) 162, 568, 1532
TspRI CASTG 3 cut(s) 1247, 1630, 2252
Vha464I CTTAAG 2 cut(s) 1090, 2030
VpaK11BI GGWCC 6 cut(s) 272, 1828, 2035, 2074, 2104, 2274
XapI RAATTY 7 cut(s) 35, 236, 728, 1655, 1759, 2144, 2570
XbaI TCTAGA 1 cut(s) 1687
XceI RCATGY 3 cut(s) 1337, 1402, 1437
XcmI CCANNNNNNNNNTGG 2 cut(s) 282, 318
XmiI GTMKAC 1 cut(s) 1140
XmnI GAANNNNTTC 4 cut(s) 128, 653, 989, 2148
XspI CTAG 3 cut(s) 614, 1688, 2213
Zsp2I ATGCAT 2 cut(s) 246, 2164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.