Rw6G019250

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000110
Physical Location & Seq
Forward (+)
38457793 .. 38461419
3627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G019250.1

Sequence Viewer

Length: 2877 bp
ATGGCCTCAGCTGCAACAGAGTTCTTGATTGGCAAAATTGAGGAAGTTCTTGAGAAGGAAGCATCCTCCATCGCAGGTGTTCGTGATCAAGTGGATGAGATTAAGCAGGAGTTGATAAGCATGAAATCCTTCCTAGAGGATGCTGAGGGAAAGAAAATAGACACGGAAGGAGAGAAGGAATGGGTAGCAAGCATTAGAAACTCTGTCTATGATGTTGAAGATATTATTGATGAATTCATGTACCGCATGCATGAGCAGCGAAATGGAGGTCGACTTGCAAGGTTGCTATGCAAAACCATTATTATTCCAAAGAATTGGTACAGGGGTCGGTTTGCAAGGTGCCTTCGCAAAACCATTCACATTCCAACCAGATTTCAATGCAGGGGTCGTTTTGTAAGGTGGCTTCGCAAATGCATTCAAATTCCAGAGTACCTTTGGTACAGGCGTCAAATTGCCAAAAAGTTGCAGAAAATCACAAAAACCATCAAAGAGATTCCAGAGAGAAAAAAGAGATATGGTGTTAGTCGTGTGGGGGGAACAGGTTCCAATGATATTCGCAATCGGGTGCAGAACCAAGCAGAGTCATCTCTTTTCATTACAGAAGATGAACTTGTAGGATTTGAAGACAAGAAGAAACTGTTAATAGGATGGCTGATGAATGAGGTAAAACATCAAATTGTTGTTTCTGTGGTCGGGATGGGAGGATCAGGAAAGACAACTCTAGTTGCCAAGATCTTCACCGATGATACTGTAAAGAAGCATTTTGAGTGTTATGCATGGATTACCGTTTCACAAACTTATGTGATTGAAGACTTGTTTCGAAGATTGATCAAGGAATTTCACCAGTCAAGGAAGGAAAACGTCCCCGGAAGTTTGAATTCCATGAGCTACAAGGAATTGCTAGAGATACTGGTGAACTACTTGGAGTTCAAAAGATATGTAGTTGTTTTGGATGATGTGTGGAATATCCTACTTTTGAGAGAAATAAGGGTATCACTTCAAGATAGACAACTTGGAAGTCGAATCCTGCTAACAACTCGAAATGATGACATAGCATTCCATTCGTATGGAGTTGAAAGCCTTGTTCATCGCATTCAACCCTTGGAGAACAATGACGCCTGGGAGCTCTTCAGCCGGAAAGCATTTTTAACTTATCATAATAAGTGTTGTCCAACAGAGCTTGAAACATTAGCTTGTGAACTAGTGGCTAAGTGTAAAGGCCTACCTTTGGCTATCGTGACTCTAGGTGGTCTTATGTCTTCAAAGAAATCATCTGCTGAGTGGAGAGAAGTGCGCAACAACTTAAATTGGCATCTTACAAACGATCACTTTCTTGAACCGGTGAAGAGCATCTTGTTGCTTAGTTACAATGATTTACCATACCGTTTGAAGCATTGTTTCTTATATTGTTCCCTTTTCCCAGAAGATCATTTGATCGGAAGATACAGGCTTGTTAGATTGTGGATAGCTGAAGGGTTTATTGAACATGTCAATGGGGTCACACTAGAAGACCTTGCAGAGAGGTATCTCATAGAGCTAACTTTTCGCAGCATGTTGCAATTTGTGAGGAAGGATTCTCAATCTAGAATCCCAGAGCAGTGTCTGATGCATGATCTGATGCGGGAGATTGCTCTCTCAACTTCAGAAAGAGAAAAGTTCGGCGTTGTATTTGATAGGAGAGGAGTAATGGAAGAAACTAGAGTTCGCCGTTTGGCAGTTCATACAACTGAAGGAGAAATTGAATTGTGCACAGGTATGTTGCAGCTTCGTACATTTCTTGTGTTTCCTTCTGGCTTACGTTCTCCATTGTTATCAAATACATGGTCTTCTGGGTTCTACTTGTTGAGAGTTCTAGATTTGGAAAATGTACCAATTGATAGGCTTCCAAACAATCTAGTGTACTTATTCAATTTAAGATATTTGAATCTTAGAGGAACTCAGATTAAAACGCTTCCAAAAGATATTGGAAGGCTCCACAACCTTCAAACCTTGGACATCAGTGACACAAACATAAAGATACTTCCTAGTGGAAGTTCAGAGTTGATAAACCTGCGCCATCTGTATATGGGACATTTCAATGATACTGGATTTGAGACCTTCATATGTGCTAGTGGGATTAGAGCATCATCACATATTTCTATGTTCAAGAAGTTGCAAGATTTATTTTTTGTTCGGTCTGATAAAAACATGATCAGACTTCTGAGAAAAATGACCCAACTTAGGATGCTTGGCATTACAAATGTAAAAGAAAGTGATGAAATAGAGCTCTGTGCCTCAATTCAGAAGATGAAGATCCTGGTCTGGTTGAATTTATCAGTAAGTGATAGCTCGGAGGGATTTCTTCGAATTGACGCACTTGCTTCACCTCCTCCGTATCTTGAGTCCCTTTTGTTACGTGGTAAACTGGAAAGCATACCATGTTGGTTTTGTTCATTACAGTGCCTCACACATATAGAGCTGCTTTGGTGTAGACTTGAAGAAGATTTACTGCCTCACATTGAGGCGCTTCCCTGTCTGGAACGTATTCTCCTTGATAATGCTTATGTTGGGAAAGAGATGAGTTTCAGTAGAGGCTTTGCAAAGCTTACACATTTGGAGTTGTTCAATTTCCGAGTATTAAGTAAGGTAAGTGTAGAAAAAGGGGTGATGCCAAATCTCCAATACCTGGGACTTCGAAGCTGCAAGAAATTGAAGACATTGCCGCATGGTCTTGAATACCTTACTCATCTCGAAAAATTGTATTTGGAGTCTGTTACAAGGAAGCTTATAGAGTCCATACGAGAAGGCGGTGTGGATCGTCCAAAGGTACAACACATCCGCATAATTGACCATCATTACAAAACATCTTCTGGGTTGTCTTACGAGAGCTTGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

958

Amino Acids

111.23

Weight (kDa)

8.9

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 7 - 88 2.3e-23 Rx N-terminal domain
NB-ARC PF00931 208 - 382 8.6e-45 NB-ARC domain
WHD_DRP PF23559 472 - 544 4e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 588 - 915 5.8e-40 Leucine-rich repeat region
LRR_8 PF13855 637 - 691 4.9e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 65
Acc16I TGCGCA 1 cut(s) 1295
Acc36I ACCTGC 2 cut(s) 65, 2058
AccB1I GGYRCC 1 cut(s) 339
AccB7I CCANNNNNTGG 1 cut(s) 2665
AccI GTMKAC 2 cut(s) 271, 2470
AciI CCGC 5 cut(s) 244, 1621, 2702, 2787, 2818
AclWI GGATC 3 cut(s) 712, 2287, 2802
AcsI RAATTY 5 cut(s) 233, 420, 836, 877, 2308
AcuI CTGAAG 4 cut(s) 1114, 1491, 1626, 1749
AcyI GRCGYC 2 cut(s) 445, 1116
AfaI GTAC 8 cut(s) 242, 320, 431, 440, 1771, 1869, 1901, 2808
AfiI CCNNNNNNNGG 3 cut(s) 1228, 2220, 2665
AflIII ACRYGT 1 cut(s) 1486
AgeI ACCGGT 1 cut(s) 1339
AhlI ACTAGT 1 cut(s) 1201
AjnI CCWGG 3 cut(s) 1118, 2295, 2664
AloI GAACNNNNNNTCC 2 cut(s) 2511, 2543
Alw21I GWGCWC 3 cut(s) 1128, 1751, 2268
Alw26I GTCTC 1 cut(s) 2087
Alw44I GTGCAC 1 cut(s) 1747
AlwI GGATC 3 cut(s) 712, 2287, 2802
AlwNI CAGNNNCTG 1 cut(s) 1603
AoxI GGCC 2 cut(s) 3, 1219
ApaLI GTGCAC 1 cut(s) 1747
ApeKI GCWGC 6 cut(s) 11, 256, 1548, 1762, 2458, 2679
ApoI RAATTY 5 cut(s) 233, 420, 836, 877, 2308
ArsI GACNNNNNNTTYG 4 cut(s) 253, 285, 1808, 1840
AsiGI ACCGGT 1 cut(s) 1339
Asp700I GAANNNNTTC 3 cut(s) 128, 541, 2523
AspLEI GCGC 3 cut(s) 1296, 2055, 2506
AsuC2I CCSGG 1 cut(s) 867
AsuHPI GGTGA 6 cut(s) 730, 833, 925, 1354, 2355, 2656
AsuII TTCGAA 3 cut(s) 820, 2344, 2674
BaeGI GKGCMC 1 cut(s) 1751
BaeI ACNNNNGTAYC 4 cut(s) 310, 343, 430, 463
BanI GGYRCC 1 cut(s) 339
BanII GRGCYC 2 cut(s) 1128, 2268
BarI GAAGNNNNNNTAC 4 cut(s) 387, 419, 2469, 2501
BbsI GAAGAC 6 cut(s) 630, 816, 1251, 1515, 1818, 2699
Bbv12I GWGCWC 3 cut(s) 1128, 1751, 2268
BbvCI CCTCAGC 2 cut(s) 7, 144
BbvI GCAGC 5 cut(s) 268, 1560, 1774, 2445, 2666
BccI CCATC 6 cut(s) 77, 491, 642, 691, 2064, 2838
BceAI ACGGC 1 cut(s) 1692
BcgI CGANNNNNNTGC 2 cut(s) 1044, 1078
BciT130I CCWGG 3 cut(s) 1120, 2297, 2666
BclI TGATCA 3 cut(s) 85, 828, 2190
BcnI CCSGG 1 cut(s) 867
BcoDI GTCTC 1 cut(s) 2087
BcuI ACTAGT 1 cut(s) 1201
BfoI RGCGCY 1 cut(s) 2507
BfuAI ACCTGC 2 cut(s) 65, 2058
BglII AGATCT 1 cut(s) 732
BisI GCNGC 7 cut(s) 12, 257, 1549, 1763, 2459, 2680, 2702
BlsI GCNGC 7 cut(s) 13, 258, 1550, 1764, 2460, 2681, 2703
Bme1390I CCNGG 4 cut(s) 867, 1120, 2297, 2666
BmiI GGNNCC 3 cut(s) 341, 544, 1973
BmrFI CCNGG 4 cut(s) 867, 1120, 2297, 2666
BmsI GCATC 9 cut(s) 71, 130, 1321, 1359, 1596, 1608, 2132, 2214, 2637
BpiI GAAGAC 6 cut(s) 630, 816, 1251, 1515, 1818, 2699
Bpu10I CCTNAGC 2 cut(s) 7, 144
Bpu14I TTCGAA 3 cut(s) 820, 2344, 2674
BpuEI CTTGAG 2 cut(s) 71, 2399
BpuMI CCSGG 1 cut(s) 867
BsaAI YACGTR 1 cut(s) 2396
BsaBI GATNNNNATC 1 cut(s) 2291
BsaHI GRCGYC 2 cut(s) 445, 1116
BsaI GGTCTC 1 cut(s) 2087
BsaJI CCNNGG 5 cut(s) 865, 1101, 1119, 1989, 2665
BsaWI WCCGGW 1 cut(s) 1339
BsaXI ACNNNNNCTCC 2 cut(s) 2511, 2541
Bsc4I CCNNNNNNNGG 3 cut(s) 1228, 2220, 2665
Bse118I RCCGGY 1 cut(s) 1339
Bse1I ACTGG 4 cut(s) 844, 915, 2089, 2409
Bse3DI GCAATG 1 cut(s) 2696
Bse8I GATNNNNATC 1 cut(s) 2291
BseBI CCWGG 3 cut(s) 1120, 2297, 2666
BseDI CCNNGG 5 cut(s) 865, 1101, 1119, 1989, 2665
BseGI GGATG 8 cut(s) 62, 100, 145, 653, 702, 958, 2229, 2814
BseJI GATNNNNATC 1 cut(s) 2291
BseLI CCNNNNNNNGG 3 cut(s) 1228, 2220, 2665
BseMI GCAATG 1 cut(s) 2696
BseMII CTCAG 5 cut(s) 21, 135, 1269, 1952, 2192
BseNI ACTGG 4 cut(s) 844, 915, 2089, 2409
BseRI GAGGAG 2 cut(s) 1695, 2358
BseSI GKGCMC 1 cut(s) 1751
BseXI GCAGC 5 cut(s) 268, 1560, 1774, 2445, 2666
BsgI GTGCAG 1 cut(s) 587
BshFI GGCC 2 cut(s) 5, 1221
BshNI GGYRCC 1 cut(s) 339
BshTI ACCGGT 1 cut(s) 1339
BsiHKAI GWGCWC 3 cut(s) 1128, 1751, 2268
BsiSI CCGG 3 cut(s) 867, 1135, 1340
BslFI GGGAC 4 cut(s) 848, 2082, 2368, 2682
BslI CCNNNNNNNGG 3 cut(s) 1228, 2220, 2665
BsmAI GTCTC 1 cut(s) 2087
BsmFI GGGAC 4 cut(s) 848, 2082, 2368, 2682
BsmI GAATGC 3 cut(s) 414, 1055, 1092
BsnI GGCC 2 cut(s) 5, 1221
Bso31I GGTCTC 1 cut(s) 2087
Bsp119I TTCGAA 3 cut(s) 820, 2344, 2674
Bsp1286I GDGCHC 3 cut(s) 1128, 1751, 2268
BspACI CCGC 5 cut(s) 244, 1621, 2702, 2787, 2818
BspANI GGCC 2 cut(s) 5, 1221
BspCNI CTCAG 5 cut(s) 20, 136, 1270, 1951, 2193
BspLI GGNNCC 3 cut(s) 341, 544, 1973
BspMI ACCTGC 2 cut(s) 65, 2058
BspPI GGATC 3 cut(s) 712, 2287, 2802
BspQI GCTCTTC 2 cut(s) 1133, 1340
BspT104I TTCGAA 3 cut(s) 820, 2344, 2674
BspT107I GGYRCC 1 cut(s) 339
BspTNI GGTCTC 1 cut(s) 2087
BsrDI GCAATG 1 cut(s) 2696
BsrFI RCCGGY 1 cut(s) 1339
BsrI ACTGG 4 cut(s) 844, 915, 2089, 2409
BssAI RCCGGY 1 cut(s) 1339
BssECI CCNNGG 5 cut(s) 865, 1101, 1119, 1989, 2665
BssNI GRCGYC 2 cut(s) 445, 1116
BssT1I CCWWGG 2 cut(s) 1101, 1989
Bst2UI CCWGG 3 cut(s) 1120, 2297, 2666
Bst4CI ACNGT 5 cut(s) 639, 751, 787, 1385, 2439
Bst6I CTCTTC 2 cut(s) 1133, 1340
BstACI GRCGYC 2 cut(s) 445, 1116
BstBAI YACGTR 1 cut(s) 2396
BstBI TTCGAA 3 cut(s) 820, 2344, 2674
BstC8I GCNNGC 2 cut(s) 190, 248
BstDEI CTNAG 9 cut(s) 7, 144, 1209, 1278, 1361, 1928, 1938, 2201, 2219
BstF5I GGATG 8 cut(s) 62, 100, 145, 653, 702, 958, 2229, 2814
BstH2I RGCGCY 1 cut(s) 2507
BstHHI GCGC 3 cut(s) 1296, 2055, 2506
BstMAI GTCTC 1 cut(s) 2087
BstMWI GCNNNNNNNGC 2 cut(s) 11, 256
BstNI CCWGG 3 cut(s) 1120, 2297, 2666
BstNSI RCATGY 3 cut(s) 250, 1490, 1555
BstSCI CCNGG 4 cut(s) 865, 1118, 2295, 2664
BstSLI GKGCMC 1 cut(s) 1751
BstV1I GCAGC 5 cut(s) 268, 1560, 1774, 2445, 2666
BstV2I GAAGAC 6 cut(s) 630, 816, 1251, 1515, 1818, 2699
BstX2I RGATCY 2 cut(s) 732, 2292
BstXI CCANNNNNNTGG 2 cut(s) 315, 1067
BstYI RGATCY 2 cut(s) 732, 2292
BsuRI GGCC 2 cut(s) 5, 1221
BtgZI GCGATG 2 cut(s) 55, 1073
BtsCI GGATG 8 cut(s) 62, 100, 145, 653, 702, 958, 2229, 2814
BtsI GCAGTG 1 cut(s) 1604
BtsIMutI CAGTG 3 cut(s) 1604, 2005, 2444
BveI ACCTGC 2 cut(s) 65, 2058
Cac8I GCNNGC 2 cut(s) 190, 248
CaiI CAGNNNCTG 1 cut(s) 1603
CfoI GCGC 3 cut(s) 1296, 2055, 2506
Cfr10I RCCGGY 1 cut(s) 1339
CseI GACGC 3 cut(s) 434, 1124, 2360
Csp6I GTAC 8 cut(s) 241, 319, 430, 439, 1770, 1868, 1900, 2807
CspAI ACCGGT 1 cut(s) 1339
CviQI GTAC 8 cut(s) 241, 319, 430, 439, 1770, 1868, 1900, 2807
DdeI CTNAG 9 cut(s) 7, 144, 1209, 1278, 1361, 1928, 1938, 2201, 2219
Eam1104I CTCTTC 2 cut(s) 1133, 1340
EarI CTCTTC 2 cut(s) 1133, 1340
Ecl136II GAGCTC 2 cut(s) 1126, 2266
Eco130I CCWWGG 2 cut(s) 1101, 1989
Eco147I AGGCCT 1 cut(s) 1221
Eco24I GRGCYC 2 cut(s) 1128, 2268
Eco31I GGTCTC 1 cut(s) 2087
Eco53kI GAGCTC 2 cut(s) 1126, 2266
Eco57I CTGAAG 4 cut(s) 1114, 1491, 1626, 1749
EcoICRI GAGCTC 2 cut(s) 1126, 2266
EcoRI GAATTC 2 cut(s) 233, 877
EcoRII CCWGG 3 cut(s) 1118, 2295, 2664
EcoT14I CCWWGG 2 cut(s) 1101, 1989
EcoT22I ATGCAT 4 cut(s) 252, 416, 778, 1611
EcoT38I GRGCYC 2 cut(s) 1128, 2268
ErhI CCWWGG 2 cut(s) 1101, 1989
FalI AAGNNNNNCTT 4 cut(s) 594, 626, 1337, 1369
FaqI GGGAC 4 cut(s) 848, 2082, 2368, 2682
FauI CCCGC 1 cut(s) 1614
FauNDI CATATG 1 cut(s) 2102
FbaI TGATCA 3 cut(s) 85, 828, 2190
FblI GTMKAC 2 cut(s) 271, 2470
Fnu4HI GCNGC 7 cut(s) 12, 257, 1549, 1763, 2459, 2680, 2702
FokI GGATG 8 cut(s) 49, 107, 152, 660, 709, 965, 2236, 2801
FriOI GRGCYC 2 cut(s) 1128, 2268
Fsp4HI GCNGC 7 cut(s) 12, 257, 1549, 1763, 2459, 2680, 2702
FspI TGCGCA 1 cut(s) 1295
GlaI GCGC 3 cut(s) 1295, 2054, 2505
GluI GCNGC 7 cut(s) 12, 257, 1549, 1763, 2459, 2680, 2702
HaeII RGCGCY 1 cut(s) 2507
HaeIII GGCC 2 cut(s) 5, 1221
HapII CCGG 3 cut(s) 867, 1135, 1340
HgaI GACGC 3 cut(s) 434, 1124, 2360
HhaI GCGC 3 cut(s) 1296, 2055, 2506
Hin1I GRCGYC 2 cut(s) 445, 1116
Hin6I GCGC 3 cut(s) 1294, 2053, 2504
HinP1I GCGC 3 cut(s) 1294, 2053, 2504
HincII GTYRAC 1 cut(s) 272
HindII GTYRAC 1 cut(s) 272
HindIII AAGCTT 2 cut(s) 2582, 2762
HpaII CCGG 3 cut(s) 867, 1135, 1340
HphI GGTGA 6 cut(s) 730, 833, 925, 1354, 2355, 2656
Hpy166II GTNNAC 7 cut(s) 272, 916, 1199, 1749, 1900, 2402, 2471
Hpy8I GTNNAC 7 cut(s) 272, 916, 1199, 1749, 1900, 2402, 2471
HpyCH4III ACNGT 5 cut(s) 639, 751, 787, 1385, 2439
HpyCH4IV ACGT 4 cut(s) 861, 1798, 2395, 2521
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 256
HpyF3I CTNAG 9 cut(s) 7, 144, 1209, 1278, 1361, 1928, 1938, 2201, 2219
HpySE526I ACGT 4 cut(s) 861, 1798, 2395, 2521
Hsp92I GRCGYC 2 cut(s) 445, 1116
HspAI GCGC 3 cut(s) 1294, 2053, 2504
Ksp22I TGATCA 3 cut(s) 85, 828, 2190
LguI GCTCTTC 2 cut(s) 1133, 1340
LmnI GCTCC 2 cut(s) 1123, 1977
Lsp1109I GCAGC 5 cut(s) 268, 1560, 1774, 2445, 2666
LweI GCATC 9 cut(s) 71, 130, 1321, 1359, 1596, 1608, 2132, 2214, 2637
MaeII ACGT 4 cut(s) 861, 1798, 2395, 2521
MaeIII GTNAC 6 cut(s) 1237, 1364, 1498, 2000, 2391, 2752
MfeI CAATTG 1 cut(s) 1872
MflI RGATCY 2 cut(s) 732, 2292
MhlI GDGCHC 3 cut(s) 1128, 1751, 2268
MlyI GAGTC 5 cut(s) 590, 1234, 2390, 2756, 2780
MmeI TCCRAC 2 cut(s) 389, 1196
Mph1103I ATGCAT 4 cut(s) 252, 416, 778, 1611
MroXI GAANNNNTTC 3 cut(s) 128, 541, 2523
MseI TTAA 7 cut(s) 102, 641, 1148, 1304, 1913, 1944, 2618
MslI CAYNNNNRTG 5 cut(s) 1065, 1491, 1754, 2076, 2437
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 3 cut(s) 867, 1135, 1340
MspR9I CCNGG 4 cut(s) 867, 1120, 2297, 2666
MunI CAATTG 1 cut(s) 1872
Mva1269I GAATGC 3 cut(s) 414, 1055, 1092
MvaI CCWGG 3 cut(s) 1120, 2297, 2666
MwoI GCNNNNNNNGC 2 cut(s) 11, 256
NciI CCSGG 1 cut(s) 867
NdeI CATATG 1 cut(s) 2102
NlaIV GGNNCC 3 cut(s) 341, 544, 1973
NmuCI GTSAC 3 cut(s) 1237, 1498, 2000
NsbI TGCGCA 1 cut(s) 1295
NsiI ATGCAT 4 cut(s) 252, 416, 778, 1611
NspI RCATGY 3 cut(s) 250, 1490, 1555
NspV TTCGAA 3 cut(s) 820, 2344, 2674
PaeI GCATGC 1 cut(s) 250
PaqCI CACCTGC 1 cut(s) 65
PceI AGGCCT 1 cut(s) 1221
PciI ACATGT 1 cut(s) 1486
PciSI GCTCTTC 2 cut(s) 1133, 1340
PctI GAATGC 3 cut(s) 414, 1055, 1092
PdmI GAANNNNTTC 3 cut(s) 128, 541, 2523
PfeI GAWTC 5 cut(s) 493, 1023, 1574, 1587, 1924
PflMI CCANNNNNTGG 1 cut(s) 2665
PinAI ACCGGT 1 cut(s) 1339
PkrI GCNGC 7 cut(s) 13, 258, 1550, 1764, 2460, 2681, 2703
PleI GAGTC 5 cut(s) 589, 1234, 2389, 2755, 2779
PpsI GAGTC 5 cut(s) 589, 1234, 2389, 2755, 2779
Ppu21I YACGTR 1 cut(s) 2396
PscI ACATGT 1 cut(s) 1486
Psp124BI GAGCTC 2 cut(s) 1128, 2268
Psp6I CCWGG 3 cut(s) 1118, 2295, 2664
PspGI CCWGG 3 cut(s) 1118, 2295, 2664
PspN4I GGNNCC 3 cut(s) 341, 544, 1973
PstNI CAGNNNCTG 1 cut(s) 1603
PsuI RGATCY 2 cut(s) 732, 2292
PvuII CAGCTG 1 cut(s) 11
RsaI GTAC 8 cut(s) 242, 320, 431, 440, 1771, 1869, 1901, 2808
RsaNI GTAC 8 cut(s) 241, 319, 430, 439, 1770, 1868, 1900, 2807
RseI CAYNNNNRTG 5 cut(s) 1065, 1491, 1754, 2076, 2437
SacI GAGCTC 2 cut(s) 1128, 2268
SalI GTCGAC 1 cut(s) 270
SapI GCTCTTC 2 cut(s) 1133, 1340
SaqAI TTAA 7 cut(s) 102, 641, 1148, 1304, 1913, 1944, 2618
SatI GCNGC 7 cut(s) 12, 257, 1549, 1763, 2459, 2680, 2702
SchI GAGTC 5 cut(s) 590, 1234, 2390, 2756, 2780
ScrFI CCNGG 4 cut(s) 867, 1120, 2297, 2666
SduI GDGCHC 3 cut(s) 1128, 1751, 2268
SfaNI GCATC 9 cut(s) 71, 130, 1321, 1359, 1596, 1608, 2132, 2214, 2637
SfuI TTCGAA 3 cut(s) 820, 2344, 2674
SmiMI CAYNNNNRTG 5 cut(s) 1065, 1491, 1754, 2076, 2437
SmlI CTYRAG 2 cut(s) 50, 2378
SmoI CTYRAG 2 cut(s) 50, 2378
SpeI ACTAGT 1 cut(s) 1201
SphI GCATGC 1 cut(s) 250
SseBI AGGCCT 1 cut(s) 1221
SsiI CCGC 5 cut(s) 244, 1621, 2702, 2787, 2818
SstI GAGCTC 2 cut(s) 1128, 2268
StuI AGGCCT 1 cut(s) 1221
StyD4I CCNGG 4 cut(s) 865, 1118, 2295, 2664
StyI CCWWGG 2 cut(s) 1101, 1989
TaaI ACNGT 5 cut(s) 639, 751, 787, 1385, 2439
TaiI ACGT 4 cut(s) 864, 1801, 2398, 2524
TaqI TCGA 7 cut(s) 271, 820, 1021, 1039, 2344, 2674, 2730
TaqII GACCGA 1 cut(s) 2163
TatI WGTACW 1 cut(s) 1899
TauI GCSGC 1 cut(s) 2704
TfiI GAWTC 5 cut(s) 493, 1023, 1574, 1587, 1924
Tru1I TTAA 7 cut(s) 102, 641, 1148, 1304, 1913, 1944, 2618
Tru9I TTAA 7 cut(s) 102, 641, 1148, 1304, 1913, 1944, 2618
TscAI CASTG 3 cut(s) 1604, 2005, 2444
TseFI GTSAC 3 cut(s) 1237, 1498, 2000
TseI GCWGC 6 cut(s) 11, 256, 1548, 1762, 2458, 2679
Tsp45I GTSAC 3 cut(s) 1237, 1498, 2000
TspGWI ACGGA 2 cut(s) 179, 2361
TspRI CASTG 3 cut(s) 1604, 2005, 2444
Van91I CCANNNNNTGG 1 cut(s) 2665
VneI GTGCAC 1 cut(s) 1747
XapI RAATTY 5 cut(s) 233, 420, 836, 877, 2308
XbaI TCTAGA 2 cut(s) 1583, 1852
XceI RCATGY 3 cut(s) 250, 1490, 1555
XcmI CCANNNNNNNNNTGG 1 cut(s) 432
XmiI GTMKAC 2 cut(s) 271, 2470
XmnI GAANNNNTTC 3 cut(s) 128, 541, 2523
Zsp2I ATGCAT 4 cut(s) 252, 416, 778, 1611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.