Rroxscaffold_1G00059270

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000110
Physical Location & Seq
Reverse (-)
81287404 .. 81290981
3578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059270.1

Sequence Viewer

Length: 2721 bp
ATGGCCTCATCTGTAACGGACCTGTTAATCGGAAAACTTGTGACGATTCTTGAGAACGAAGGAACAACCATAGCTGGTGTTCGTGATGAAGTTGATAATGTTAAGGAGGAGTTTCAAAGCATGAAAGCTTTCCTAGTGGATGCGGAGGCCAACAAAGCAAAAACTGAAGGAGAGAAATTGTGGGTTGCAAGAATCAGAGACTTAGCCTATGATGTTGAAGATATCATTGATGAATACATGTATCACATGTATGAGAAGCAAAGTGGGGGTCGACTTTCAAGGGGGCTACACAAAACCATTCGCGCTCCATTTAATATTTGGTTCAGGCATAAAATTGCAAAGAAGTTGCAGAAAATTACTGAAATGATCAAAGCCATTCCAGAGAGGAATCAAAGGTACAGTGTTGGCCTTGTAGGAGGAGCAACTACGTCTGAAGATATTCACAAATTGGTGCAGTTGCAAGCGGAGTCTTCGTTTTTCATTATGGAGGACGAACTGGTTGGGATTGAAGGCAAGAAGAAAATACTAATGGGATGGCTCATGGATGAAGAGCAACACCAAACTATTATATCTGTGGTTGGCATGGGAGGTTCTGGAAAGACAACTCTAGTTGCCAAGACCTTCAACAATGAAAGAGTGAAGAGACATTTCGATTGTTATGCATGGATTACCGTTTCCCTAACTTATGATGTGGTACATTTGTTTCGAAGCTTGATCATGGAACTCCACAAACCAAGAAAGGGAGGTATCCCTGAAAATTTGAATGTCATGAGCAGCAGAGAATTGATAGAGATACTGACAAACTACTTGGAGTCCAAAAGATACCTGGTTGTTCTAGATGACGTGTGGGACATAAAACTTTGGAGAGAAATAAAGGTATCACTTCAAGATAGGCAACTTGGAAGTCGAATCATAGTTACAACTCGAAAAGAAGATGTAGCATCCAATTCTTTTGGAGTTAAAAGCCATGTTCACCATATTCAACCCTTGCTAAAGAATGAGGCTTGGGAGCTCTTCTGCAAGAAAGCATTCTCATCTAATGAGCACAAAACTTGTCCACCTGAGTTTATGTCATTAGCTTGCCAACTTGTGGAAAAGTGTGAAGGCCTTCCACTGGCTATTGTGGCTTTAGGTGGTCTTATGTCTTCTAAGAAGTCACTGGATCAATGGCAACAAGTCCTCAATAACTTCAATTGGCATCTACATAAAGTTTCATTGCTAGACTCTATGAAAAACATCTTGCTGCTGAGTTTCAATGATTTGCCATCTCAATTGAAACATTGCTTCCTCTATTGCTCTCTATTCCCAGAAGATTTTGTAATTCACAGTAAAAGGCTTACTAGATTGTGGATAGCCGAAGGATTTGTTGAACATGTGAAAGGGGTCACACCAGAAGAAGTTGCAGACGACTATCTTATGGAGCTCTGCTTCCGTAGCTTGTTACAAGTTGTAGAGAGAAATGAAACAGGAAGTCCAAAACCAGTTAAGATGCATGATCTGATGCGAGAGCTTGCTTTGTCGATAGCTGAGAAGGAAAAGTTTGGTCATGTATATGACGACAGAGAAGTAATGGAAGAGATCTCAACCCGTCGCTTGTCAATTCACAAATTGGAAGGAGAAATTAAATCATGGCCGAGTATGTCAAAGATTCGTTCTCTTCTTGTCTTTGCAACTGACATGTCCTCATTGTCTTTCTCAAATGCACTGGTTTCTCGATTCAAATTGTTGAGGACTCTAGACTTGGAGGGTGTCCAAATTTATAAACTGCCGGATGTAGTTGTTTCCTTGTTCAACTTGAGATATTTAAATTTGGAGGGCACTTTAATCAAAGAACTTCCAGAGTCCATAGGGCGTCTCTACAACCTTCAATTTCTGAGCATCAAGGATACTAAGATAGAGTCACTTCCACAAGGAATTGCAAGGTTGCTGAATCTGCGCTATCTAATTATGTATCGCTACTCTAGAAACGCTTTGGACTTCAAATGGGCGAGTGGGACAAAAGCGCCATCAAAAATTTATAAGTTGCAGAAATTGCAATTTTTATCAATAATGGAATCAGAAGGGGACACTATTAGACTTGTCGGCAACATGACTCAACTTAGGAACATCGGCATATCGAATGTGAAAGAAAGAGATGAGAATGACCTTTGGGTCTCAATTGAAAAGTTGAATATGCTTCAATCTTTGTTTTTAATGGCAAGTAATGAAGAGGAAATCCTTCCGGTGAAAGCACAATGTCCTCCTCTTCCGCGCCTTCAAAAGCTTTATTTGTATGGGAGACTGCAAAACGTAATACCTTGGTTTTCTTCACTACATAGCCTCACATCTCTGTCTCTGCATTGGTCCAGACTTGAAGAAGATTTACTACCTCAAATTGAAGCACTGCCCAATCTGACAACGCTTTATCTTTGTAATGCATATGTTGGGGAAGAATTGTGTTTCCGCAGAGGCTTTGTAAAGCTGGAGATCTTGAATTTGCGGAATTTTGATTCAATGAAAAAGATAACTATAGAAAAAGGGGTGATGCCAAATCTCTGGTACTTGTGGGTTGAAAGCTGCATGGAGTTGAAGACAGTGCCACAGGGCCTTGAATACCTTTCAAATCTACAGACTTTAGGCCTGAGAATTGTTCCGATAGAAATTATAACGCCCATTCGGAAAGGAGGTGCAGACCGCTTTAAGCTTTTCCAGAATCTATCAAGTCTGACAAGTATATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

906

Amino Acids

104.34

Weight (kDa)

8.06

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 90 9.8e-23 Rx N-terminal domain
NB-ARC PF00931 172 - 347 6.1e-47 NB-ARC domain
WHD_DRP PF23559 434 - 505 1.8e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 549 - 874 6.3e-43 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1761, 2019, 2645
AasI GACNNNNNNGTC 1 cut(s) 2150
AccB7I CCANNNNNTGG 1 cut(s) 1090
AccI GTMKAC 1 cut(s) 271
AccII CGCG 2 cut(s) 303, 2251
AciI CCGC 6 cut(s) 143, 464, 2249, 2443, 2479, 2674
AclWI GGATC 1 cut(s) 1170
AcoI YGGCCR 1 cut(s) 1631
AcsI RAATTY 6 cut(s) 757, 1755, 1807, 2013, 2473, 2482
AcuI CTGAAG 2 cut(s) 186, 453
AcyI GRCGYC 1 cut(s) 1852
AfaI GTAC 3 cut(s) 398, 696, 2540
AfiI CCNNNNNNNGG 3 cut(s) 740, 1090, 1114
AflIII ACRYGT 5 cut(s) 237, 246, 843, 1372, 1677
AjiI CACGTC 1 cut(s) 844
AjnI CCWGG 1 cut(s) 825
Alw21I GWGCWC 3 cut(s) 1014, 1047, 1425
Alw26I GTCTC 6 cut(s) 192, 637, 1859, 2158, 2272, 2337
AlwI GGATC 1 cut(s) 1170
AoxI GGCC 7 cut(s) 3, 147, 406, 1105, 1631, 2584, 2617
ApeKI GCWGC 3 cut(s) 774, 1243, 2556
ApoI RAATTY 6 cut(s) 757, 1755, 1807, 2013, 2473, 2482
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2217
AspLEI GCGC 4 cut(s) 305, 1938, 2005, 2253
AspS9I GGNCC 3 cut(s) 19, 2343, 2584
AsuHPI GGTGA 3 cut(s) 965, 2236, 2533
AsuII TTCGAA 1 cut(s) 706
AvaII GGWCC 2 cut(s) 19, 2343
BaeGI GKGCMC 1 cut(s) 1820
BaeI ACNNNNGTAYC 2 cut(s) 686, 719
BanII GRGCYC 2 cut(s) 1014, 1425
BarI GAAGNNNNNNTAC 4 cut(s) 2346, 2349, 2378, 2381
BbsI GAAGAC 3 cut(s) 462, 1137, 2576
Bbv12I GWGCWC 3 cut(s) 1014, 1047, 1425
BbvI GCAGC 3 cut(s) 786, 1230, 2543
BccI CCATC 3 cut(s) 528, 1273, 2014
BcgI CGANNNNNNTGC 2 cut(s) 641, 675
BciT130I CCWGG 1 cut(s) 827
BciVI GTATCC 2 cut(s) 758, 1879
BclI TGATCA 2 cut(s) 366, 714
BcoDI GTCTC 6 cut(s) 192, 637, 1859, 2158, 2272, 2337
BfaI CTAG 7 cut(s) 134, 608, 836, 1220, 1341, 1736, 1962
BfmI CTRYAG 2 cut(s) 2508, 2606
BfoI RGCGCY 1 cut(s) 2006
BfuI GTATCC 2 cut(s) 758, 1879
BglII AGATCT 2 cut(s) 1578, 2466
BisI GCNGC 3 cut(s) 775, 1244, 2557
BlsI GCNGC 3 cut(s) 776, 1245, 2558
Bme1390I CCNGG 1 cut(s) 827
Bme18I GGWCC 2 cut(s) 19, 2343
BmgBI CACGTC 1 cut(s) 844
BmgT120I GGNCC 3 cut(s) 19, 2343, 2584
BmrFI CCNGG 1 cut(s) 827
BmsI GCATC 7 cut(s) 130, 950, 1207, 1479, 1491, 1887, 2514
BpiI GAAGAC 3 cut(s) 462, 1137, 2576
BpmI CTGGAG 1 cut(s) 2483
Bpu14I TTCGAA 1 cut(s) 706
BpuEI CTTGAG 2 cut(s) 71, 1816
BsaHI GRCGYC 1 cut(s) 1852
BsaI GGTCTC 1 cut(s) 2158
BsaJI CCNNGG 1 cut(s) 2297
BsaWI WCCGGW 1 cut(s) 2221
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 3 cut(s) 740, 1090, 1114
Bse1I ACTGG 5 cut(s) 501, 1119, 1164, 1481, 1710
Bse3DI GCAATG 2 cut(s) 1214, 1279
BseBI CCWGG 1 cut(s) 827
BseDI CCNNGG 1 cut(s) 2297
BseGI GGATG 5 cut(s) 145, 539, 550, 941, 1777
BseLI CCNNNNNNNGG 3 cut(s) 740, 1090, 1114
BseMI GCAATG 2 cut(s) 1214, 1279
BseMII CTCAG 5 cut(s) 1053, 1238, 1518, 1865, 2612
BseNI ACTGG 5 cut(s) 501, 1119, 1164, 1481, 1710
BseRI GAGGAG 3 cut(s) 122, 432, 2232
BseSI GKGCMC 1 cut(s) 1820
BseXI GCAGC 3 cut(s) 786, 1230, 2543
BsgI GTGCAG 2 cut(s) 473, 2688
Bsh1236I CGCG 2 cut(s) 303, 2251
BshFI GGCC 7 cut(s) 5, 149, 408, 1107, 1633, 2586, 2619
BsiHKAI GWGCWC 3 cut(s) 1014, 1047, 1425
BsiSI CCGG 2 cut(s) 1769, 2222
BslFI GGGAC 3 cut(s) 863, 2008, 2078
BslI CCNNNNNNNGG 3 cut(s) 740, 1090, 1114
BsmAI GTCTC 6 cut(s) 192, 637, 1859, 2158, 2272, 2337
BsmBI CGTCTC 1 cut(s) 1859
BsmFI GGGAC 3 cut(s) 863, 2008, 2078
BsmI GAATGC 1 cut(s) 1028
BsnI GGCC 7 cut(s) 5, 149, 408, 1107, 1633, 2586, 2619
Bso31I GGTCTC 1 cut(s) 2158
Bsp119I TTCGAA 1 cut(s) 706
Bsp1286I GDGCHC 4 cut(s) 1014, 1047, 1425, 1820
Bsp143I GATC 6 cut(s) 366, 714, 1162, 1495, 1578, 2466
BspACI CCGC 6 cut(s) 143, 464, 2249, 2443, 2479, 2674
BspANI GGCC 7 cut(s) 5, 149, 408, 1107, 1633, 2586, 2619
BspCNI CTCAG 5 cut(s) 1054, 1239, 1519, 1866, 2613
BspFNI CGCG 2 cut(s) 303, 2251
BspHI TCATGA 1 cut(s) 768
BspPI GGATC 1 cut(s) 1170
BspQI GCTCTTC 2 cut(s) 543, 1019
BspT104I TTCGAA 1 cut(s) 706
BspTNI GGTCTC 1 cut(s) 2158
BsrDI GCAATG 2 cut(s) 1214, 1279
BsrI ACTGG 5 cut(s) 501, 1119, 1164, 1481, 1710
BssECI CCNNGG 1 cut(s) 2297
BssMI GATC 6 cut(s) 366, 714, 1162, 1495, 1578, 2466
BssNI GRCGYC 1 cut(s) 1852
BssT1I CCWWGG 1 cut(s) 2297
Bst2UI CCWGG 1 cut(s) 827
Bst4CI ACNGT 4 cut(s) 401, 673, 1328, 2575
Bst6I CTCTTC 7 cut(s) 543, 635, 1019, 1569, 1662, 2202, 2250
BstACI GRCGYC 1 cut(s) 1852
BstAPI GCANNNNNTGC 1 cut(s) 2032
BstBI TTCGAA 1 cut(s) 706
BstC8I GCNNGC 3 cut(s) 462, 1081, 1512
BstDEI CTNAG 9 cut(s) 202, 1062, 1149, 1247, 1527, 1874, 1890, 2099, 2621
BstF5I GGATG 5 cut(s) 145, 539, 550, 941, 1777
BstFNI CGCG 2 cut(s) 303, 2251
BstH2I RGCGCY 1 cut(s) 2006
BstHHI GCGC 4 cut(s) 305, 1938, 2005, 2253
BstKTI GATC 6 cut(s) 369, 717, 1165, 1498, 1581, 2469
BstMAI GTCTC 6 cut(s) 192, 637, 1859, 2158, 2272, 2337
BstMBI GATC 6 cut(s) 366, 714, 1162, 1495, 1578, 2466
BstMWI GCNNNNNNNGC 5 cut(s) 155, 1124, 1434, 1933, 2032
BstNI CCWGG 1 cut(s) 827
BstNSI RCATGY 4 cut(s) 241, 250, 1376, 1681
BstSCI CCNGG 1 cut(s) 825
BstSFI CTRYAG 2 cut(s) 2508, 2606
BstSLI GKGCMC 1 cut(s) 1820
BstUI CGCG 2 cut(s) 303, 2251
BstV1I GCAGC 3 cut(s) 786, 1230, 2543
BstV2I GAAGAC 3 cut(s) 462, 1137, 2576
BstX2I RGATCY 2 cut(s) 1578, 2466
BstXI CCANNNNNNTGG 1 cut(s) 2535
BstYI RGATCY 2 cut(s) 1578, 2466
BsuI GTATCC 2 cut(s) 758, 1879
BsuRI GGCC 7 cut(s) 5, 149, 408, 1107, 1633, 2586, 2619
BtrI CACGTC 1 cut(s) 844
BtsCI GGATG 5 cut(s) 145, 539, 550, 941, 1777
BtsI GCAGTG 1 cut(s) 2381
BtsIMutI CAGTG 6 cut(s) 406, 1112, 1157, 1703, 2381, 2580
Cac8I GCNNGC 3 cut(s) 462, 1081, 1512
CciI TCATGA 1 cut(s) 768
CfoI GCGC 4 cut(s) 305, 1938, 2005, 2253
Cfr13I GGNCC 3 cut(s) 19, 2343, 2584
CseI GACGC 1 cut(s) 1841
CsiI ACCWGGT 1 cut(s) 825
Csp6I GTAC 3 cut(s) 397, 695, 2539
CviQI GTAC 3 cut(s) 397, 695, 2539
DdeI CTNAG 9 cut(s) 202, 1062, 1149, 1247, 1527, 1874, 1890, 2099, 2621
DpnI GATC 6 cut(s) 368, 716, 1164, 1497, 1580, 2468
DpnII GATC 6 cut(s) 366, 714, 1162, 1495, 1578, 2466
DraI TTTAAA 1 cut(s) 1806
DrdI GACNNNNNNGTC 1 cut(s) 2150
DseDI GACNNNNNNGTC 1 cut(s) 2150
EaeI YGGCCR 1 cut(s) 1631
Eam1104I CTCTTC 7 cut(s) 543, 635, 1019, 1569, 1662, 2202, 2250
EarI CTCTTC 7 cut(s) 543, 635, 1019, 1569, 1662, 2202, 2250
Ecl136II GAGCTC 2 cut(s) 1012, 1423
Eco130I CCWWGG 1 cut(s) 2297
Eco147I AGGCCT 2 cut(s) 1107, 2619
Eco24I GRGCYC 2 cut(s) 1014, 1425
Eco31I GGTCTC 1 cut(s) 2158
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 2 cut(s) 19, 2343
Eco53kI GAGCTC 2 cut(s) 1012, 1423
Eco57I CTGAAG 2 cut(s) 186, 453
EcoICRI GAGCTC 2 cut(s) 1012, 1423
EcoO109I RGGNCCY 1 cut(s) 2584
EcoRII CCWGG 1 cut(s) 825
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 2297
EcoT22I ATGCAT 3 cut(s) 664, 1494, 2419
EcoT38I GRGCYC 2 cut(s) 1014, 1425
ErhI CCWWGG 1 cut(s) 2297
Esp3I CGTCTC 1 cut(s) 1859
FaqI GGGAC 3 cut(s) 863, 2008, 2078
FauNDI CATATG 1 cut(s) 2419
FbaI TGATCA 2 cut(s) 366, 714
FblI GTMKAC 1 cut(s) 271
Fnu4HI GCNGC 3 cut(s) 775, 1244, 2557
FokI GGATG 5 cut(s) 152, 546, 557, 928, 1784
FriOI GRGCYC 2 cut(s) 1014, 1425
Fsp4HI GCNGC 3 cut(s) 775, 1244, 2557
FspBI CTAG 7 cut(s) 134, 608, 836, 1220, 1341, 1736, 1962
GlaI GCGC 4 cut(s) 304, 1937, 2004, 2252
GluI GCNGC 3 cut(s) 775, 1244, 2557
GsuI CTGGAG 1 cut(s) 2483
HaeII RGCGCY 1 cut(s) 2006
HaeIII GGCC 7 cut(s) 5, 149, 408, 1107, 1633, 2586, 2619
HapII CCGG 2 cut(s) 1769, 2222
HgaI GACGC 1 cut(s) 1841
HhaI GCGC 4 cut(s) 305, 1938, 2005, 2253
Hin1I GRCGYC 1 cut(s) 1852
Hin6I GCGC 4 cut(s) 303, 1936, 2003, 2251
HinP1I GCGC 4 cut(s) 303, 1936, 2003, 2251
HincII GTYRAC 1 cut(s) 272
HindII GTYRAC 1 cut(s) 272
HindIII AAGCTT 4 cut(s) 126, 709, 2261, 2681
HpaII CCGG 2 cut(s) 1769, 2222
HphI GGTGA 3 cut(s) 965, 2236, 2533
Hpy166II GTNNAC 3 cut(s) 272, 973, 1058
Hpy8I GTNNAC 3 cut(s) 272, 973, 1058
Hpy99I CGWCG 1 cut(s) 1593
HpyCH4III ACNGT 4 cut(s) 401, 673, 1328, 2575
HpyCH4IV ACGT 3 cut(s) 428, 843, 2289
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 1124, 1434, 1933, 2032
HpyF3I CTNAG 9 cut(s) 202, 1062, 1149, 1247, 1527, 1874, 1890, 2099, 2621
HpySE526I ACGT 3 cut(s) 428, 843, 2289
Hsp92I GRCGYC 1 cut(s) 1852
HspAI GCGC 4 cut(s) 303, 1936, 2003, 2251
Ksp22I TGATCA 2 cut(s) 366, 714
Kzo9I GATC 6 cut(s) 366, 714, 1162, 1495, 1578, 2466
LguI GCTCTTC 2 cut(s) 543, 1019
LmnI GCTCC 4 cut(s) 310, 419, 1009, 1420
Lsp1109I GCAGC 3 cut(s) 786, 1230, 2543
LweI GCATC 7 cut(s) 130, 950, 1207, 1479, 1491, 1887, 2514
MabI ACCWGGT 1 cut(s) 825
MaeI CTAG 7 cut(s) 134, 608, 836, 1220, 1341, 1736, 1962
MaeII ACGT 3 cut(s) 428, 843, 2289
MaeIII GTNAC 7 cut(s) 13, 40, 916, 1155, 1384, 1440, 1899
MalI GATC 6 cut(s) 368, 716, 1164, 1497, 1580, 2468
MboI GATC 6 cut(s) 366, 714, 1162, 1495, 1578, 2466
MfeI CAATTG 3 cut(s) 1192, 1271, 2157
MflI RGATCY 2 cut(s) 1578, 2466
MhlI GDGCHC 4 cut(s) 1014, 1047, 1425, 1820
MlyI GAGTC 7 cut(s) 476, 821, 1217, 1726, 1850, 1907, 2086
Mph1103I ATGCAT 3 cut(s) 664, 1494, 2419
MroXI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2217
MslI CAYNNNNRTG 2 cut(s) 249, 1551
MspI CCGG 2 cut(s) 1769, 2222
MspR9I CCNGG 1 cut(s) 827
MunI CAATTG 3 cut(s) 1192, 1271, 2157
Mva1269I GAATGC 1 cut(s) 1028
MvaI CCWGG 1 cut(s) 827
MvnI CGCG 2 cut(s) 303, 2251
MwoI GCNNNNNNNGC 5 cut(s) 155, 1124, 1434, 1933, 2032
NdeI CATATG 1 cut(s) 2419
NdeII GATC 6 cut(s) 366, 714, 1162, 1495, 1578, 2466
NmeAIII GCCGAG 1 cut(s) 1659
NmuCI GTSAC 4 cut(s) 40, 1155, 1384, 1899
NsiI ATGCAT 3 cut(s) 664, 1494, 2419
NspI RCATGY 4 cut(s) 241, 250, 1376, 1681
NspV TTCGAA 1 cut(s) 706
PagI TCATGA 1 cut(s) 768
PceI AGGCCT 2 cut(s) 1107, 2619
PciI ACATGT 4 cut(s) 237, 246, 1372, 1677
PciSI GCTCTTC 2 cut(s) 543, 1019
PctI GAATGC 1 cut(s) 1028
PdmI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2217
PflMI CCANNNNNTGG 1 cut(s) 1090
PkrI GCNGC 3 cut(s) 776, 1245, 2558
PleI GAGTC 7 cut(s) 475, 820, 1217, 1726, 1849, 1906, 2086
PpsI GAGTC 7 cut(s) 475, 820, 1217, 1726, 1849, 1906, 2086
PscI ACATGT 4 cut(s) 237, 246, 1372, 1677
PsiI TTATAA 3 cut(s) 1761, 2019, 2645
Psp124BI GAGCTC 2 cut(s) 1014, 1425
Psp6I CCWGG 1 cut(s) 825
PspGI CCWGG 1 cut(s) 825
PspPI GGNCC 3 cut(s) 19, 2343, 2584
PsuI RGATCY 2 cut(s) 1578, 2466
RsaI GTAC 3 cut(s) 398, 696, 2540
RsaNI GTAC 3 cut(s) 397, 695, 2539
RseI CAYNNNNRTG 2 cut(s) 249, 1551
SacI GAGCTC 2 cut(s) 1014, 1425
SalI GTCGAC 1 cut(s) 270
SapI GCTCTTC 2 cut(s) 543, 1019
SatI GCNGC 3 cut(s) 775, 1244, 2557
Sau3AI GATC 6 cut(s) 366, 714, 1162, 1495, 1578, 2466
Sau96I GGNCC 3 cut(s) 19, 2343, 2584
SchI GAGTC 7 cut(s) 476, 821, 1217, 1726, 1850, 1907, 2086
ScrFI CCNGG 1 cut(s) 827
SduI GDGCHC 4 cut(s) 1014, 1047, 1425, 1820
SexAI ACCWGGT 1 cut(s) 825
SfaNI GCATC 7 cut(s) 130, 950, 1207, 1479, 1491, 1887, 2514
SfcI CTRYAG 2 cut(s) 2508, 2606
SfuI TTCGAA 1 cut(s) 706
SinI GGWCC 2 cut(s) 19, 2343
SmiI ATTTAAAT 1 cut(s) 1806
SmiMI CAYNNNNRTG 2 cut(s) 249, 1551
SmlI CTYRAG 2 cut(s) 50, 1795
SmoI CTYRAG 2 cut(s) 50, 1795
SseBI AGGCCT 2 cut(s) 1107, 2619
SsiI CCGC 6 cut(s) 143, 464, 2249, 2443, 2479, 2674
SspI AATATT 1 cut(s) 316
SspMI CTAG 7 cut(s) 134, 608, 836, 1220, 1341, 1736, 1962
SstI GAGCTC 2 cut(s) 1014, 1425
StuI AGGCCT 2 cut(s) 1107, 2619
StyD4I CCNGG 1 cut(s) 825
StyI CCWWGG 1 cut(s) 2297
SwaI ATTTAAAT 1 cut(s) 1806
TaaI ACNGT 4 cut(s) 401, 673, 1328, 2575
TaiI ACGT 3 cut(s) 431, 846, 2292
TaqI TCGA 8 cut(s) 271, 651, 706, 907, 925, 1520, 1714, 2117
TscAI CASTG 6 cut(s) 406, 1119, 1164, 1710, 2388, 2580
TseFI GTSAC 4 cut(s) 40, 1155, 1384, 1899
TseI GCWGC 3 cut(s) 774, 1243, 2556
Tsp45I GTSAC 4 cut(s) 40, 1155, 1384, 1899
TspGWI ACGGA 2 cut(s) 32, 1421
TspRI CASTG 6 cut(s) 406, 1119, 1164, 1710, 2388, 2580
Van91I CCANNNNNTGG 1 cut(s) 1090
VpaK11BI GGWCC 2 cut(s) 19, 2343
XapI RAATTY 6 cut(s) 757, 1755, 1807, 2013, 2473, 2482
XbaI TCTAGA 3 cut(s) 835, 1735, 1961
XceI RCATGY 4 cut(s) 241, 250, 1376, 1681
XcmI CCANNNNNNNNNTGG 2 cut(s) 315, 823
XmiI GTMKAC 1 cut(s) 271
XmnI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2217
XspI CTAG 7 cut(s) 134, 608, 836, 1220, 1341, 1736, 1962
Zsp2I ATGCAT 3 cut(s) 664, 1494, 2419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.