FvH4_3g11505

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000110
Physical Location & Seq
Forward (+)
6800068 .. 6803181
3114 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11505.t1

Sequence Viewer

Length: 2670 bp
ATGGCTTTATCTGCAACAATCTTATTAATTGGCAAACTTGGAGCAATTCTTGAGAACGAAGGAAGAACCATAGCCGGTGTTCGTGATCAAGTTGACAAGATTAAGCTGGAGCTTCTAAGCATGAAAGCTTTCCTGGAGGACGCGGAGGCCAACAAAGCAAAAACTGAAGGAGAGAAATTGTGGGTTGCAAGCATCAGAGACCTGGGCTATGATGTTGAAGATATCATTGATGAATTCATACGTAAAACTGCAAAGAGATTAGAAAAGATCACTGAAAAGATCAAAGACATTCCTGAGAGGAATAAAAGGTATGATGTTGGCCATGTAGGAGGAACAAGTACTTCTGAAGATATTCACAGATGGGCGCAGAACCAAGCGTACTCTTCTTTTTTCATGACGGAAGACGAGCTGGTTGGGATTGAAGGCAAGAAGCAAATACTAATGGAACGGCTCATGGATGAAGAGCAACACCAAACTGTTATATCTGTAGTTGGGATGGGAGGTTCTGGAAAGACAACTCTAGTTGCCAAGATCTTCAACAATGAACGGGTAAAGAGACATTTTGATTGTTATGCATGGATTACCGTTTCCCAAACTTATGATGTGTTAGATGTGTTTCGAAGCTTGATCAGGGAACTCTACAAATCAAGGAAGGAAGATTTCCCTGCAAATTTGAATGCCATGAGCCGCATAGAATTGCTAGAGTTACTAGTTGACAACTTGGAGTACAAAAGATACTTGGTTGTTCTAGATGACGTGTGGGACTTAAGAGCTTGGAGAGAAATAAAGGTATCACTTCAAGATAGGCAACTTGGAAGTCGAATCATAGTTACAACTCGAAAAGAAGATGTAGCATGCAATTCATTAGGAGTTAAAAACCATGTTCACCATATTCAACCCCTGCTAAAGAACGAGGCTTGGGAGCTCTTCAGCAGGAAAGCATTCTCAACTAATCAGCATAAAACCTGTCCTGCTGAACTTGAATCATTAGCTTGCCAACTTGTGGATAAGTGTGAAGGCCTTCCTCTGGCCATTGTGTCTCTAGGTGGTCTTATGTCTTCTAAGAAATCATTGGATCAATGGGACACAGTTCTCAACACCTTCAATTGGTATCTACATAACAATTCATCACTAGACCCTGTTAGACAAATGTTGCTGCTGAGTTTCAATGATTTGCCATCTCAACTGAAACATTGCTTCCTCTGTTGTTCCCTTTTTCCAGAAGATTTTGTGATTAAAAGAAAAAGGCTCACTAGATTGTGGATAGCCGAAGGATTTGTTGAACATGTGAAAGGGGTCATGCCAGAAGAAATTGCAGACGAATATCTCATGGAGCTCTGTTTCCGTAGCATGTTACAAGTTGTTGAAAGAAATGAAACAGGAAGGCCAAAACAGATTAAGATGCATGATCTGATGCGAGAGCTTGCTTTGTCGACAGCCGAGAAGGAAAAGTTTGGTCTTGTATACCATGGGAAAGAAGAAATGGAAGAGATGTCAAGCCGTCGCTTGTCAATCCACAAATTGGAAGGAGAGATTAAATCATGGCCGGGTATGTCAAACATTCGTTCTCTTCTTGTCTTTGCCACTAACATGTCCTCGTTGTCTTTCTCGAATGCACTGCTTTGTCGATTCAAGTTGTTGAGGAGTCTAGACTTGGAGGATGTTCAAATTGATAAACTGCCGGATGCAGTTGCTTACTTGTTCAACTTGAGATATTTAAATTTGAAGGGCACTTTAATAGAGCAACTTCCAGAGACCATAGGGCGTCTCTGCAACCTTCAATTTTTGAACATCATGGATACTAAGATAGAGTCACTTCCAAAAGGAATTGCGAAATTACTAAACCTGCGCCATCTTATCATGTATCGTTACTCTGTCAGCCATGTGTGTTTTAAGCGTAGTAGTGGGACAAGAGCACCCTCAGATATCTGTAAGTTGCAGAAATTGCAGTCTTTGTTCTGCATTGAGTCAGAAGGAGACATTATCAGACATATCGGTAACATGTCTCAACTTACAGGCATTGGCATAACAAATGTAAAAGAAGTAGATGAAATTGACCTTTGGAACTCAGTTAAGAAGTTGACTGTGCTCCACACTCTGGTTTTAATGACAAGTTCTGAAGAGGAAATTCTTCCGGTGAAAGCAGAATGTCCACCTCTTCCGCACCTTCGAACGCTTACTTTTGTTGGCAGACTCATGCAAAGCGCAATCCCTTGGTTTTTTTCACTGCAGAGCCTCACATCTCTGTTTCTGCATTGGTCAAGACTTGAAGACGATTTACTACCTCATATTGAAGCACTGCCCAATCTGACAACGCTTTATCTCTGTAATGCATATGTGGGAGAAGAGTTGTGTTTCCGCAGAGGCTTTGTAAAGCTGGTGAGATTGGAGTTGTTGAATTTTGCATCGTTGAAAAAGATAACTATAGAAAAAGAGGTGATGCCAAATCTCCAGTACTTGTGGGTTGAATGCTGCATGGAGTTGAAGACAGTGCCACTGGGCCTTGAATACCTTTCTTATCTACAAACTTTAGAGCTGGGATTTGTTCCGATGGAAGTTATAGAGTCTATTCGGAAAGGAGGTCTGGATCGCTCTAAGGTACAACACATTCCTGAGATCAGACATTATTTCCAACTGTCATCCAAGCTGGTCTTCGAAAACTTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

890

Amino Acids

102.25

Weight (kDa)

6.49

Isoelectric Point (pI)

52.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 87 5.3e-21 Rx N-terminal domain
NB-ARC PF00931 143 - 318 6.9e-47 NB-ARC domain
WHD_DRP PF23559 405 - 476 1.6e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 521 - 846 2.5e-36 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1854
AccB7I CCANNNNNTGG 3 cut(s) 1003, 1522, 2098
AccI GTMKAC 2 cut(s) 1433, 1464
AccII CGCG 1 cut(s) 143
AciI CCGC 4 cut(s) 143, 688, 2162, 2359
AclWI GGATC 2 cut(s) 1083, 2595
AcoI YGGCCR 3 cut(s) 319, 1029, 1544
AcsI RAATTY 5 cut(s) 233, 670, 1720, 2127, 2398
AcuI CTGAAG 4 cut(s) 186, 366, 913, 2139
AcyI GRCGYC 1 cut(s) 1765
AfaI GTAC 5 cut(s) 340, 380, 728, 2456, 2601
AfiI CCNNNNNNNGG 5 cut(s) 1003, 1027, 1107, 1522, 2098
AflII CTTAAG 1 cut(s) 766
AflIII ACRYGT 4 cut(s) 756, 1285, 1590, 2001
AhlI ACTAGT 1 cut(s) 709
AjiI CACGTC 1 cut(s) 757
AjnI CCWGG 2 cut(s) 132, 201
AjuI GAANNNNNNNTTGG 2 cut(s) 2636, 2668
AloI GAACNNNNNNTCC 2 cut(s) 487, 519
Alw21I GWGCWC 4 cut(s) 927, 1338, 1918, 2091
Alw26I GTCTC 7 cut(s) 192, 550, 1044, 1748, 1772, 1971, 2010
AlwI GGATC 2 cut(s) 1083, 2595
AoxI GGCC 8 cut(s) 147, 319, 1018, 1029, 1385, 1544, 2500, 2664
ApeKI GCWGC 2 cut(s) 1156, 2472
ApoI RAATTY 5 cut(s) 233, 670, 1720, 2127, 2398
AseI ATTAAT 1 cut(s) 26
Asp700I GAANNNNTTC 5 cut(s) 128, 351, 941, 1020, 2130
AspLEI GCGC 3 cut(s) 367, 1851, 2207
AspS9I GGNCC 1 cut(s) 2500
AsuC2I CCSGG 1 cut(s) 1548
AsuHPI GGTGA 4 cut(s) 878, 2149, 2392, 2449
AsuII TTCGAA 3 cut(s) 619, 2170, 2655
BaeGI GKGCMC 1 cut(s) 1733
BalI TGGCCA 2 cut(s) 321, 1031
BanII GRGCYC 2 cut(s) 927, 1338
BarI GAAGNNNNNNTAC 2 cut(s) 2262, 2294
BbsI GAAGAC 5 cut(s) 408, 1050, 2277, 2492, 2644
Bbv12I GWGCWC 4 cut(s) 927, 1338, 1918, 2091
BbvI GCAGC 2 cut(s) 1143, 2459
BccI CCATC 5 cut(s) 354, 490, 1186, 1860, 2545
BceAI ACGGC 2 cut(s) 464, 1485
BcgI CGANNNNNNTGC 2 cut(s) 1600, 1634
BciT130I CCWGG 2 cut(s) 134, 203
BciVI GTATCC 1 cut(s) 1792
BclI TGATCA 2 cut(s) 85, 627
BcnI CCSGG 1 cut(s) 1548
BcoDI GTCTC 7 cut(s) 192, 550, 1044, 1748, 1772, 1971, 2010
BcuI ACTAGT 1 cut(s) 709
BfaI CTAG 9 cut(s) 521, 701, 710, 749, 1043, 1133, 1254, 1649, 2668
BfmI CTRYAG 3 cut(s) 486, 2228, 2424
BfrI CTTAAG 1 cut(s) 766
BfuAI ACCTGC 1 cut(s) 1854
BfuI GTATCC 1 cut(s) 1792
BglII AGATCT 1 cut(s) 531
BisI GCNGC 3 cut(s) 688, 1157, 2473
BlsI GCNGC 3 cut(s) 689, 1158, 2474
BmcAI AGTACT 2 cut(s) 340, 2456
Bme1390I CCNGG 3 cut(s) 134, 203, 1548
BmgBI CACGTC 1 cut(s) 757
BmgT120I GGNCC 1 cut(s) 2500
BmrFI CCNGG 3 cut(s) 134, 203, 1548
BmrI ACTGGG 1 cut(s) 2507
BmsI GCATC 6 cut(s) 201, 1392, 1404, 1675, 2414, 2430
BmuI ACTGGG 1 cut(s) 2507
BpiI GAAGAC 5 cut(s) 408, 1050, 2277, 2492, 2644
BpmI CTGGAG 3 cut(s) 128, 155, 2435
Bpu14I TTCGAA 3 cut(s) 619, 2170, 2655
BpuEI CTTGAG 2 cut(s) 71, 1729
BpuMI CCSGG 1 cut(s) 1548
BsaAI YACGTR 1 cut(s) 242
BsaHI GRCGYC 1 cut(s) 1765
BsaI GGTCTC 2 cut(s) 192, 1748
BsaJI CCNNGG 3 cut(s) 202, 1468, 2213
BsaWI WCCGGW 1 cut(s) 2134
Bsc4I CCNNNNNNNGG 5 cut(s) 1003, 1027, 1107, 1522, 2098
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 2 cut(s) 2452, 2502
Bse3DI GCAATG 1 cut(s) 1192
BseBI CCWGG 2 cut(s) 134, 203
BseDI CCNNGG 3 cut(s) 202, 1468, 2213
BseGI GGATG 5 cut(s) 463, 501, 1666, 1690, 2639
BseLI CCNNNNNNNGG 5 cut(s) 1003, 1027, 1107, 1522, 2098
BseMI GCAATG 1 cut(s) 1192
BseMII CTCAG 5 cut(s) 285, 1151, 1935, 2082, 2604
BseNI ACTGG 2 cut(s) 2452, 2502
BseRI GAGGAG 1 cut(s) 1657
BseSI GKGCMC 1 cut(s) 1733
BseXI GCAGC 2 cut(s) 1143, 2459
BseYI CCCAGC 1 cut(s) 2536
Bsh1236I CGCG 1 cut(s) 143
BshFI GGCC 8 cut(s) 149, 321, 1020, 1031, 1387, 1546, 2502, 2666
BsiHKAI GWGCWC 4 cut(s) 927, 1338, 1918, 2091
BsiSI CCGG 4 cut(s) 75, 1547, 1682, 2135
BslFI GGGAC 3 cut(s) 776, 1097, 1921
BslI CCNNNNNNNGG 5 cut(s) 1003, 1027, 1107, 1522, 2098
BsmAI GTCTC 7 cut(s) 192, 550, 1044, 1748, 1772, 1971, 2010
BsmBI CGTCTC 1 cut(s) 1772
BsmFI GGGAC 3 cut(s) 776, 1097, 1921
BsmI GAATGC 4 cut(s) 682, 941, 1618, 2474
BsnI GGCC 8 cut(s) 149, 321, 1020, 1031, 1387, 1546, 2502, 2666
Bso31I GGTCTC 2 cut(s) 192, 1748
Bsp119I TTCGAA 3 cut(s) 619, 2170, 2655
Bsp1286I GDGCHC 5 cut(s) 927, 1338, 1733, 1918, 2091
Bsp143I GATC 9 cut(s) 85, 267, 279, 531, 627, 1075, 1408, 2587, 2616
Bsp19I CCATGG 1 cut(s) 1468
BspACI CCGC 4 cut(s) 143, 688, 2162, 2359
BspANI GGCC 8 cut(s) 149, 321, 1020, 1031, 1387, 1546, 2502, 2666
BspCNI CTCAG 5 cut(s) 286, 1152, 1934, 2081, 2605
BspFNI CGCG 1 cut(s) 143
BspHI TCATGA 1 cut(s) 393
BspMAI CTGCAG 1 cut(s) 2232
BspMI ACCTGC 1 cut(s) 1854
BspPI GGATC 2 cut(s) 1083, 2595
BspQI GCTCTTC 2 cut(s) 456, 932
BspT104I TTCGAA 3 cut(s) 619, 2170, 2655
BspTI CTTAAG 1 cut(s) 766
BspTNI GGTCTC 2 cut(s) 192, 1748
BsrDI GCAATG 1 cut(s) 1192
BsrFI RCCGGY 1 cut(s) 74
BsrI ACTGG 2 cut(s) 2452, 2502
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 3 cut(s) 202, 1468, 2213
BssMI GATC 9 cut(s) 85, 267, 279, 531, 627, 1075, 1408, 2587, 2616
BssNAI GTATAC 1 cut(s) 1465
BssNI GRCGYC 1 cut(s) 1765
BssT1I CCWWGG 2 cut(s) 1468, 2213
Bst1107I GTATAC 1 cut(s) 1465
Bst2UI CCWGG 2 cut(s) 134, 203
Bst4CI ACNGT 6 cut(s) 478, 586, 1090, 2086, 2491, 2637
Bst6I CTCTTC 8 cut(s) 388, 456, 932, 1482, 1575, 2115, 2163, 2340
BstACI GRCGYC 1 cut(s) 1765
BstAFI CTTAAG 1 cut(s) 766
BstAPI GCANNNNNTGC 1 cut(s) 1945
BstBAI YACGTR 1 cut(s) 242
BstBI TTCGAA 3 cut(s) 619, 2170, 2655
BstC8I GCNNGC 4 cut(s) 190, 856, 994, 1425
BstDEI CTNAG 9 cut(s) 116, 294, 1062, 1160, 1803, 1921, 2068, 2595, 2613
BstDSI CCRYGG 1 cut(s) 1468
BstF5I GGATG 5 cut(s) 463, 501, 1666, 1690, 2639
BstFNI CGCG 1 cut(s) 143
BstHHI GCGC 3 cut(s) 367, 1851, 2207
BstKTI GATC 9 cut(s) 88, 270, 282, 534, 630, 1078, 1411, 2590, 2619
BstMAI GTCTC 7 cut(s) 192, 550, 1044, 1748, 1772, 1971, 2010
BstMBI GATC 9 cut(s) 85, 267, 279, 531, 627, 1075, 1408, 2587, 2616
BstMWI GCNNNNNNNGC 3 cut(s) 11, 155, 1945
BstNI CCWGG 2 cut(s) 134, 203
BstNSI RCATGY 5 cut(s) 858, 1289, 1354, 1594, 2005
BstSCI CCNGG 3 cut(s) 132, 201, 1546
BstSFI CTRYAG 3 cut(s) 486, 2228, 2424
BstSLI GKGCMC 1 cut(s) 1733
BstSNI TACGTA 1 cut(s) 242
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 2 cut(s) 1143, 2459
BstV2I GAAGAC 5 cut(s) 408, 1050, 2277, 2492, 2644
BstX2I RGATCY 1 cut(s) 531
BstYI RGATCY 1 cut(s) 531
BstZ17I GTATAC 1 cut(s) 1465
BsuI GTATCC 1 cut(s) 1792
BsuRI GGCC 8 cut(s) 149, 321, 1020, 1031, 1387, 1546, 2502, 2666
BtgI CCRYGG 1 cut(s) 1468
BtrI CACGTC 1 cut(s) 757
BtsCI GGATG 5 cut(s) 463, 501, 1666, 1690, 2639
BtsI GCAGTG 3 cut(s) 1616, 2225, 2297
BtsIMutI CAGTG 6 cut(s) 270, 1616, 2225, 2297, 2495, 2496
BveI ACCTGC 1 cut(s) 1854
Cac8I GCNNGC 4 cut(s) 190, 856, 994, 1425
CciI TCATGA 1 cut(s) 393
CfoI GCGC 3 cut(s) 367, 1851, 2207
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 2500
CseI GACGC 2 cut(s) 149, 1754
Csp6I GTAC 5 cut(s) 339, 379, 727, 2455, 2600
CviQI GTAC 5 cut(s) 339, 379, 727, 2455, 2600
DdeI CTNAG 9 cut(s) 116, 294, 1062, 1160, 1803, 1921, 2068, 2595, 2613
DpnI GATC 9 cut(s) 87, 269, 281, 533, 629, 1077, 1410, 2589, 2618
DpnII GATC 9 cut(s) 85, 267, 279, 531, 627, 1075, 1408, 2587, 2616
DraI TTTAAA 1 cut(s) 1719
EaeI YGGCCR 3 cut(s) 319, 1029, 1544
Eam1104I CTCTTC 8 cut(s) 388, 456, 932, 1482, 1575, 2115, 2163, 2340
EarI CTCTTC 8 cut(s) 388, 456, 932, 1482, 1575, 2115, 2163, 2340
Ecl136II GAGCTC 2 cut(s) 925, 1336
Eco105I TACGTA 1 cut(s) 242
Eco130I CCWWGG 2 cut(s) 1468, 2213
Eco147I AGGCCT 1 cut(s) 1020
Eco24I GRGCYC 2 cut(s) 927, 1338
Eco31I GGTCTC 2 cut(s) 192, 1748
Eco32I GATATC 2 cut(s) 223, 1927
Eco53kI GAGCTC 2 cut(s) 925, 1336
Eco57I CTGAAG 4 cut(s) 186, 366, 913, 2139
EcoICRI GAGCTC 2 cut(s) 925, 1336
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 2 cut(s) 132, 201
EcoRV GATATC 2 cut(s) 223, 1927
EcoT14I CCWWGG 2 cut(s) 1468, 2213
EcoT22I ATGCAT 3 cut(s) 577, 1407, 2335
EcoT38I GRGCYC 2 cut(s) 927, 1338
ErhI CCWWGG 2 cut(s) 1468, 2213
Esp3I CGTCTC 1 cut(s) 1772
FalI AAGNNNNNCTT 2 cut(s) 2636, 2668
FaqI GGGAC 3 cut(s) 776, 1097, 1921
FauNDI CATATG 1 cut(s) 2335
FbaI TGATCA 2 cut(s) 85, 627
FblI GTMKAC 2 cut(s) 1433, 1464
Fnu4HI GCNGC 3 cut(s) 688, 1157, 2473
FokI GGATG 5 cut(s) 470, 508, 1673, 1697, 2626
FriOI GRGCYC 2 cut(s) 927, 1338
Fsp4HI GCNGC 3 cut(s) 688, 1157, 2473
FspBI CTAG 9 cut(s) 521, 701, 710, 749, 1043, 1133, 1254, 1649, 2668
GlaI GCGC 3 cut(s) 366, 1850, 2206
GluI GCNGC 3 cut(s) 688, 1157, 2473
GsaI CCCAGC 1 cut(s) 2540
GsuI CTGGAG 3 cut(s) 128, 155, 2435
HaeIII GGCC 8 cut(s) 149, 321, 1020, 1031, 1387, 1546, 2502, 2666
HapII CCGG 4 cut(s) 75, 1547, 1682, 2135
HgaI GACGC 2 cut(s) 149, 1754
HhaI GCGC 3 cut(s) 367, 1851, 2207
Hin1I GRCGYC 1 cut(s) 1765
Hin6I GCGC 3 cut(s) 365, 1849, 2205
HinP1I GCGC 3 cut(s) 365, 1849, 2205
HincII GTYRAC 4 cut(s) 94, 715, 1434, 2082
HindII GTYRAC 4 cut(s) 94, 715, 1434, 2082
HindIII AAGCTT 2 cut(s) 126, 622
HinfI GANTC 8 cut(s) 822, 983, 1629, 1645, 1811, 1967, 2193, 2564
HpaII CCGG 4 cut(s) 75, 1547, 1682, 2135
HphI GGTGA 4 cut(s) 878, 2149, 2392, 2449
Hpy166II GTNNAC 7 cut(s) 94, 715, 886, 1434, 1465, 2082, 2153
Hpy8I GTNNAC 7 cut(s) 94, 715, 886, 1434, 1465, 2082, 2153
Hpy99I CGWCG 1 cut(s) 1506
HpyCH4III ACNGT 6 cut(s) 478, 586, 1090, 2086, 2491, 2637
HpyCH4IV ACGT 2 cut(s) 241, 756
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 155, 1945
HpyF3I CTNAG 9 cut(s) 116, 294, 1062, 1160, 1803, 1921, 2068, 2595, 2613
HpySE526I ACGT 2 cut(s) 241, 756
Hsp92I GRCGYC 1 cut(s) 1765
HspAI GCGC 3 cut(s) 365, 1849, 2205
Ksp22I TGATCA 2 cut(s) 85, 627
Kzo9I GATC 9 cut(s) 85, 267, 279, 531, 627, 1075, 1408, 2587, 2616
LguI GCTCTTC 2 cut(s) 456, 932
LmnI GCTCC 5 cut(s) 41, 109, 922, 1333, 2094
Lsp1109I GCAGC 2 cut(s) 1143, 2459
LweI GCATC 6 cut(s) 201, 1392, 1404, 1675, 2414, 2430
MaeI CTAG 9 cut(s) 521, 701, 710, 749, 1043, 1133, 1254, 1649, 2668
MaeII ACGT 2 cut(s) 241, 756
MaeIII GTNAC 6 cut(s) 705, 829, 1353, 1812, 1868, 1997
MalI GATC 9 cut(s) 87, 269, 281, 533, 629, 1077, 1410, 2589, 2618
MboI GATC 9 cut(s) 85, 267, 279, 531, 627, 1075, 1408, 2587, 2616
MfeI CAATTG 1 cut(s) 1105
MflI RGATCY 1 cut(s) 531
MhlI GDGCHC 5 cut(s) 927, 1338, 1733, 1918, 2091
MlsI TGGCCA 2 cut(s) 321, 1031
MluNI TGGCCA 2 cut(s) 321, 1031
MlyI GAGTC 5 cut(s) 1654, 1820, 1976, 2187, 2573
MmeI TCCRAC 1 cut(s) 2656
Mox20I TGGCCA 2 cut(s) 321, 1031
Mph1103I ATGCAT 3 cut(s) 577, 1407, 2335
MroXI GAANNNNTTC 5 cut(s) 128, 351, 941, 1020, 2130
MscI TGGCCA 2 cut(s) 321, 1031
MslI CAYNNNNRTG 1 cut(s) 1589
Msp20I TGGCCA 2 cut(s) 321, 1031
MspCI CTTAAG 1 cut(s) 766
MspI CCGG 4 cut(s) 75, 1547, 1682, 2135
MspR9I CCNGG 3 cut(s) 134, 203, 1548
MunI CAATTG 1 cut(s) 1105
Mva1269I GAATGC 4 cut(s) 682, 941, 1618, 2474
MvaI CCWGG 2 cut(s) 134, 203
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 3 cut(s) 11, 155, 1945
NciI CCSGG 1 cut(s) 1548
NcoI CCATGG 1 cut(s) 1468
NdeI CATATG 1 cut(s) 2335
NdeII GATC 9 cut(s) 85, 267, 279, 531, 627, 1075, 1408, 2587, 2616
NmeAIII GCCGAG 1 cut(s) 1465
NmuCI GTSAC 1 cut(s) 1812
NsiI ATGCAT 3 cut(s) 577, 1407, 2335
NspI RCATGY 5 cut(s) 858, 1289, 1354, 1594, 2005
NspV TTCGAA 3 cut(s) 619, 2170, 2655
PaeI GCATGC 1 cut(s) 858
PagI TCATGA 1 cut(s) 393
PceI AGGCCT 1 cut(s) 1020
PciI ACATGT 3 cut(s) 1285, 1590, 2001
PciSI GCTCTTC 2 cut(s) 456, 932
PctI GAATGC 4 cut(s) 682, 941, 1618, 2474
PdmI GAANNNNTTC 5 cut(s) 128, 351, 941, 1020, 2130
PfeI GAWTC 3 cut(s) 822, 983, 1629
PflMI CCANNNNNTGG 3 cut(s) 1003, 1522, 2098
PfoI TCCNGGA 1 cut(s) 132
PkrI GCNGC 3 cut(s) 689, 1158, 2474
PleI GAGTC 5 cut(s) 1653, 1819, 1975, 2187, 2572
PpsI GAGTC 5 cut(s) 1653, 1819, 1975, 2187, 2572
Ppu21I YACGTR 1 cut(s) 242
PscI ACATGT 3 cut(s) 1285, 1590, 2001
PshBI ATTAAT 1 cut(s) 26
Psp124BI GAGCTC 2 cut(s) 927, 1338
Psp6I CCWGG 2 cut(s) 132, 201
PspFI CCCAGC 1 cut(s) 2536
PspGI CCWGG 2 cut(s) 132, 201
PspPI GGNCC 1 cut(s) 2500
PsrI GAACNNNNNNTAC 2 cut(s) 362, 394
PstI CTGCAG 1 cut(s) 2232
PsuI RGATCY 1 cut(s) 531
RsaI GTAC 5 cut(s) 340, 380, 728, 2456, 2601
RsaNI GTAC 5 cut(s) 339, 379, 727, 2455, 2600
RseI CAYNNNNRTG 1 cut(s) 1589
SacI GAGCTC 2 cut(s) 927, 1338
SalI GTCGAC 1 cut(s) 1432
SapI GCTCTTC 2 cut(s) 456, 932
SatI GCNGC 3 cut(s) 688, 1157, 2473
Sau3AI GATC 9 cut(s) 85, 267, 279, 531, 627, 1075, 1408, 2587, 2616
Sau96I GGNCC 1 cut(s) 2500
ScaI AGTACT 2 cut(s) 340, 2456
SchI GAGTC 5 cut(s) 1654, 1820, 1976, 2187, 2573
ScrFI CCNGG 3 cut(s) 134, 203, 1548
SduI GDGCHC 5 cut(s) 927, 1338, 1733, 1918, 2091
SfaNI GCATC 6 cut(s) 201, 1392, 1404, 1675, 2414, 2430
SfcI CTRYAG 3 cut(s) 486, 2228, 2424
SfuI TTCGAA 3 cut(s) 619, 2170, 2655
SmiI ATTTAAAT 1 cut(s) 1719
SmiMI CAYNNNNRTG 1 cut(s) 1589
SmlI CTYRAG 3 cut(s) 50, 766, 1708
SmoI CTYRAG 3 cut(s) 50, 766, 1708
SnaBI TACGTA 1 cut(s) 242
SpeI ACTAGT 1 cut(s) 709
SphI GCATGC 1 cut(s) 858
SseBI AGGCCT 1 cut(s) 1020
SsiI CCGC 4 cut(s) 143, 688, 2162, 2359
SspMI CTAG 9 cut(s) 521, 701, 710, 749, 1043, 1133, 1254, 1649, 2668
SstI GAGCTC 2 cut(s) 927, 1338
StuI AGGCCT 1 cut(s) 1020
StyD4I CCNGG 3 cut(s) 132, 201, 1546
StyI CCWWGG 2 cut(s) 1468, 2213
SwaI ATTTAAAT 1 cut(s) 1719
TaaI ACNGT 6 cut(s) 478, 586, 1090, 2086, 2491, 2637
TaiI ACGT 2 cut(s) 244, 759
TaqI TCGA 8 cut(s) 619, 820, 838, 1433, 1610, 1627, 2170, 2655
TatI WGTACW 3 cut(s) 338, 726, 2454
TauI GCSGC 1 cut(s) 690
TfiI GAWTC 3 cut(s) 822, 983, 1629
TscAI CASTG 6 cut(s) 277, 1623, 2232, 2304, 2496, 2502
TseFI GTSAC 1 cut(s) 1812
TseI GCWGC 2 cut(s) 1156, 2472
Tsp45I GTSAC 1 cut(s) 1812
TspGWI ACGGA 2 cut(s) 413, 1334
TspRI CASTG 6 cut(s) 277, 1623, 2232, 2304, 2496, 2502
Van91I CCANNNNNTGG 3 cut(s) 1003, 1522, 2098
Vha464I CTTAAG 1 cut(s) 766
VspI ATTAAT 1 cut(s) 26
XapI RAATTY 5 cut(s) 233, 670, 1720, 2127, 2398
XbaI TCTAGA 2 cut(s) 748, 1648
XceI RCATGY 5 cut(s) 858, 1289, 1354, 1594, 2005
XmiI GTMKAC 2 cut(s) 1433, 1464
XmnI GAANNNNTTC 5 cut(s) 128, 351, 941, 1020, 2130
XspI CTAG 9 cut(s) 521, 701, 710, 749, 1043, 1133, 1254, 1649, 2668
ZrmI AGTACT 2 cut(s) 340, 2456
Zsp2I ATGCAT 3 cut(s) 577, 1407, 2335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.