Rorug05G0502000

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000110
Physical Location & Seq
Reverse (-)
68328548 .. 68329177
630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0502000.1

Sequence Viewer

Length: 630 bp
ATGGAACCACAAACCCAGCAACCAACCGAGGATGGAGGAAGCAACAAAGCAGCCGGGAGTAGTGCTAACTTGCTTTGTAGGCGGAGCAGGTGGACTCCCACTACAGATCATATAAAAATCCTCAATGAGCTTTTCTACAATAAGGGAGTTAGGTCCCCAACTATAGAGCAGGTTCAGAGGATCTGTCTCCAGCTGAAATGGTACGGCAAGATCGAGTTCAAGAACGTCTATTATTGGTTCGTGAACCAAAGGTCTCGGGAGAAGCAGAAGAAGAGGTCCACTTCGAATGTTCATGTGCCCATGCAAAGATCAGGGCTTGTTGGTAATGGTAATGGTACCAATTGGAAACCTGAGGATCAGTATATTAACTTCGGATCTTCTGCATCTGCTTCTGCTTCTTCAGCTGGTGTGATTGCTTTTAACGGGCAGATGGGGAACTATGGTGGTTGTGGATCTATGAACATGGAGATTGAAACCCTTCCTCTGTTCCCCATGCATGGTAAGGACATCTTTGGCAACATGAAGACTACTTCCGATGGAGGTGGCGGTCACGGTGGTGGCTCTCACATTTCTCTTGAGCTCAGCCTTTCCTGCGAGGGCAAAGCTGGAAAAAGTTTCCGGAGCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

209

Amino Acids

22.92

Weight (kDa)

9.51

Isoelectric Point (pI)

59.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 27 - 89 1.7e-08 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 78
Acc36I ACCTGC 2 cut(s) 78, 160
Acc65I GGTACC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 335
AccIII TCCGGA 1 cut(s) 618
AciI CCGC 3 cut(s) 82, 546, 625
AclWI GGATC 4 cut(s) 188, 363, 382, 460
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 2 cut(s) 203, 337
AfiI CCNNNNNNNGG 1 cut(s) 497
AgsI TTSAA 2 cut(s) 220, 473
AleI CACNNNNGTG 1 cut(s) 555
AluBI AGCT 5 cut(s) 130, 193, 404, 580, 605
AluI AGCT 5 cut(s) 130, 193, 404, 580, 605
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 2 cut(s) 191, 258
AlwI GGATC 4 cut(s) 188, 363, 382, 460
Ama87I CYCGRG 1 cut(s) 255
Aor13HI TCCGGA 1 cut(s) 618
ApeKI GCWGC 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 477
Asp718I GGTACC 1 cut(s) 335
AspS9I GGNCC 2 cut(s) 153, 276
AsuC2I CCSGG 1 cut(s) 55
AsuII TTCGAA 1 cut(s) 284
AvaI CYCGRG 1 cut(s) 255
AvaII GGWCC 2 cut(s) 153, 276
AxyI CCTNAGG 1 cut(s) 351
BaeGI GKGCMC 1 cut(s) 300
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 582
BarI GAAGNNNNNNTAC 2 cut(s) 353, 385
BbsI GAAGAC 1 cut(s) 530
Bbv12I GWGCWC 1 cut(s) 582
BbvI GCAGC 1 cut(s) 62
BccI CCATC 3 cut(s) 26, 424, 530
BceAI ACGGC 1 cut(s) 220
BcnI CCSGG 1 cut(s) 55
BcoDI GTCTC 2 cut(s) 191, 258
BfmI CTRYAG 2 cut(s) 102, 162
BfuAI ACCTGC 2 cut(s) 78, 160
BisI GCNGC 2 cut(s) 51, 625
BlpI GCTNAGC 1 cut(s) 581
BlsI GCNGC 2 cut(s) 52, 626
Bme1390I CCNGG 1 cut(s) 55
Bme18I GGWCC 2 cut(s) 153, 276
BmeT110I CYCGRG 1 cut(s) 255
BmgT120I GGNCC 2 cut(s) 153, 276
BmiI GGNNCC 4 cut(s) 6, 155, 337, 623
BmrFI CCNGG 1 cut(s) 55
BmsI GCATC 1 cut(s) 392
BpiI GAAGAC 1 cut(s) 530
BpmI CTGGAG 1 cut(s) 173
Bpu1102I GCTNAGC 1 cut(s) 581
Bpu14I TTCGAA 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 596
BpuMI CCSGG 1 cut(s) 55
BsaI GGTCTC 1 cut(s) 258
BsaJI CCNNGG 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 618
Bsc4I CCNNNNNNNGG 1 cut(s) 497
Bse21I CCTNAGG 1 cut(s) 351
BseAI TCCGGA 1 cut(s) 618
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 1 cut(s) 37
BseLI CCNNNNNNNGG 1 cut(s) 497
BseMII CTCAG 2 cut(s) 342, 595
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 1 cut(s) 62
BseYI CCCAGC 1 cut(s) 15
BshNI GGYRCC 1 cut(s) 335
BsiHKAI GWGCWC 1 cut(s) 582
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 2 cut(s) 54, 619
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 1 cut(s) 497
BsmAI GTCTC 2 cut(s) 191, 258
BsmFI GGGAC 1 cut(s) 139
Bso31I GGTCTC 1 cut(s) 258
BsoBI CYCGRG 1 cut(s) 255
Bsp119I TTCGAA 1 cut(s) 284
Bsp1286I GDGCHC 2 cut(s) 300, 582
Bsp13I TCCGGA 1 cut(s) 618
Bsp143I GATC 7 cut(s) 106, 180, 210, 308, 355, 374, 452
Bsp1720I GCTNAGC 1 cut(s) 581
BspACI CCGC 3 cut(s) 82, 546, 625
BspCNI CTCAG 2 cut(s) 343, 594
BspEI TCCGGA 1 cut(s) 618
BspLI GGNNCC 4 cut(s) 6, 155, 337, 623
BspMI ACCTGC 2 cut(s) 78, 160
BspPI GGATC 4 cut(s) 188, 363, 382, 460
BspT104I TTCGAA 1 cut(s) 284
BspT107I GGYRCC 1 cut(s) 335
BspTNI GGTCTC 1 cut(s) 258
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 7 cut(s) 106, 180, 210, 308, 355, 374, 452
Bst4CI ACNGT 1 cut(s) 554
Bst6I CTCTTC 1 cut(s) 266
BstBI TTCGAA 1 cut(s) 284
BstDEI CTNAG 2 cut(s) 351, 581
BstF5I GGATG 1 cut(s) 37
BstKTI GATC 7 cut(s) 109, 183, 213, 311, 358, 377, 455
BstMAI GTCTC 2 cut(s) 191, 258
BstMBI GATC 7 cut(s) 106, 180, 210, 308, 355, 374, 452
BstMWI GCNNNNNNNGC 3 cut(s) 79, 401, 591
BstSCI CCNGG 1 cut(s) 53
BstSFI CTRYAG 2 cut(s) 102, 162
BstSLI GKGCMC 1 cut(s) 300
BstV1I GCAGC 1 cut(s) 62
BstV2I GAAGAC 1 cut(s) 530
BstX2I RGATCY 3 cut(s) 180, 374, 452
BstYI RGATCY 3 cut(s) 180, 374, 452
Bsu36I CCTNAGG 1 cut(s) 351
BtsCI GGATG 1 cut(s) 37
BveI ACCTGC 2 cut(s) 78, 160
Cfr13I GGNCC 2 cut(s) 153, 276
Csp6I GTAC 2 cut(s) 202, 336
CviAII CATG 6 cut(s) 293, 301, 463, 493, 497, 520
CviQI GTAC 2 cut(s) 202, 336
DdeI CTNAG 2 cut(s) 351, 581
DpnI GATC 7 cut(s) 108, 182, 212, 310, 357, 376, 454
DpnII GATC 7 cut(s) 106, 180, 210, 308, 355, 374, 452
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
EciI GGCGGA 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 580
Eco24I GRGCYC 1 cut(s) 582
Eco31I GGTCTC 1 cut(s) 258
Eco47I GGWCC 2 cut(s) 153, 276
Eco53kI GAGCTC 1 cut(s) 580
Eco57I CTGAAG 1 cut(s) 384
Eco81I CCTNAGG 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 580
EcoO109I RGGNCCY 1 cut(s) 153
EcoT22I ATGCAT 1 cut(s) 498
EcoT38I GRGCYC 1 cut(s) 582
FaeI CATG 6 cut(s) 296, 304, 466, 496, 500, 523
FalI AAGNNNNNCTT 2 cut(s) 494, 526
FaqI GGGAC 1 cut(s) 139
FatI CATG 6 cut(s) 292, 300, 462, 492, 496, 519
Fnu4HI GCNGC 2 cut(s) 51, 625
FokI GGATG 1 cut(s) 44
FriOI GRGCYC 1 cut(s) 582
Fsp4HI GCNGC 2 cut(s) 51, 625
GluI GCNGC 2 cut(s) 51, 625
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 1 cut(s) 173
HapII CCGG 2 cut(s) 54, 619
Hin1II CATG 6 cut(s) 296, 304, 466, 496, 500, 523
HinfI GANTC 1 cut(s) 94
HpaII CCGG 2 cut(s) 54, 619
Hpy166II GTNNAC 3 cut(s) 93, 244, 279
Hpy188I TCNGA 3 cut(s) 177, 374, 535
Hpy188III TCNNGA 5 cut(s) 220, 241, 257, 575, 619
Hpy8I GTNNAC 3 cut(s) 93, 244, 279
HpyAV CCTTC 1 cut(s) 488
HpyCH4III ACNGT 1 cut(s) 554
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 3 cut(s) 304, 383, 496
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 401, 591
HpyF3I CTNAG 2 cut(s) 351, 581
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 6 cut(s) 296, 304, 466, 496, 500, 523
Kpn2I TCCGGA 1 cut(s) 618
KpnI GGTACC 1 cut(s) 339
Kzo9I GATC 7 cut(s) 106, 180, 210, 308, 355, 374, 452
LmnI GCTCC 2 cut(s) 84, 621
Lsp1109I GCAGC 1 cut(s) 62
LweI GCATC 1 cut(s) 392
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 548
MalI GATC 7 cut(s) 108, 182, 212, 310, 357, 376, 454
MboI GATC 7 cut(s) 106, 180, 210, 308, 355, 374, 452
MboII GAAGA 5 cut(s) 280, 283, 369, 390, 535
MfeI CAATTG 1 cut(s) 340
MflI RGATCY 3 cut(s) 180, 374, 452
MhlI GDGCHC 2 cut(s) 300, 582
MluCI AATT 1 cut(s) 340
MlyI GAGTC 1 cut(s) 88
MnlI CCTC 9 cut(s) 22, 29, 131, 171, 267, 346, 492, 533, 589
Mph1103I ATGCAT 1 cut(s) 498
MroI TCCGGA 1 cut(s) 618
MroXI GAANNNNTTC 1 cut(s) 477
MseI TTAA 2 cut(s) 366, 420
MslI CAYNNNNRTG 1 cut(s) 555
MspA1I CMGCKG 3 cut(s) 193, 404, 627
MspI CCGG 2 cut(s) 54, 619
MspR9I CCNGG 1 cut(s) 55
MunI CAATTG 1 cut(s) 340
MwoI GCNNNNNNNGC 3 cut(s) 79, 401, 591
NciI CCSGG 1 cut(s) 55
NdeII GATC 7 cut(s) 106, 180, 210, 308, 355, 374, 452
NlaIII CATG 6 cut(s) 296, 304, 466, 496, 500, 523
NlaIV GGNNCC 4 cut(s) 6, 155, 337, 623
NmuCI GTSAC 1 cut(s) 548
NsiI ATGCAT 1 cut(s) 498
NspV TTCGAA 1 cut(s) 284
OliI CACNNNNGTG 1 cut(s) 555
PaqCI CACCTGC 1 cut(s) 78
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 477
PkrI GCNGC 2 cut(s) 52, 626
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 153
Psp124BI GAGCTC 1 cut(s) 582
Psp5II RGGWCCY 1 cut(s) 153
PspFI CCCAGC 1 cut(s) 15
PspN4I GGNNCC 4 cut(s) 6, 155, 337, 623
PspPI GGNCC 2 cut(s) 153, 276
PspPPI RGGWCCY 1 cut(s) 153
PsuI RGATCY 3 cut(s) 180, 374, 452
PvuII CAGCTG 2 cut(s) 193, 404
RsaI GTAC 2 cut(s) 203, 337
RsaNI GTAC 2 cut(s) 202, 336
RseI CAYNNNNRTG 1 cut(s) 555
SacI GAGCTC 1 cut(s) 582
SaqAI TTAA 2 cut(s) 366, 420
SatI GCNGC 2 cut(s) 51, 625
Sau3AI GATC 7 cut(s) 106, 180, 210, 308, 355, 374, 452
Sau96I GGNCC 2 cut(s) 153, 276
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 55
SduI GDGCHC 2 cut(s) 300, 582
SfaNI GCATC 1 cut(s) 392
SfcI CTRYAG 2 cut(s) 102, 162
SfuI TTCGAA 1 cut(s) 284
SinI GGWCC 2 cut(s) 153, 276
SmiMI CAYNNNNRTG 1 cut(s) 555
SmlI CTYRAG 1 cut(s) 575
SmoI CTYRAG 1 cut(s) 575
Sse9I AATT 1 cut(s) 340
SsiI CCGC 3 cut(s) 82, 546, 625
SstI GAGCTC 1 cut(s) 582
StyD4I CCNGG 1 cut(s) 53
TaaI ACNGT 1 cut(s) 554
TaiI ACGT 1 cut(s) 228
TaqI TCGA 2 cut(s) 213, 284
TasI AATT 1 cut(s) 340
TauI GCSGC 1 cut(s) 627
Tru1I TTAA 2 cut(s) 366, 420
Tru9I TTAA 2 cut(s) 366, 420
TseFI GTSAC 1 cut(s) 548
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 548
TspDTI ATGAA 3 cut(s) 281, 473, 536
VpaK11BI GGWCC 2 cut(s) 153, 276
XmnI GAANNNNTTC 1 cut(s) 477
Zsp2I ATGCAT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.