Belongs to the disease resistance NB-LRR family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 2382 |
| AatII | GACGTC | 1 cut(s) | 334 |
| Acc16I | TGCGCA | 1 cut(s) | 446 |
| Acc65I | GGTACC | 1 cut(s) | 825 |
| AccB1I | GGYRCC | 1 cut(s) | 825 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1939 |
| AccI | GTMKAC | 1 cut(s) | 1182 |
| AciI | CCGC | 2 cut(s) | 467, 1409 |
| AclI | AACGTT | 1 cut(s) | 2683 |
| AclWI | GGATC | 2 cut(s) | 601, 1173 |
| AcsI | RAATTY | 8 cut(s) | 236, 725, 760, 1053, 1532, 1758, 1908, 2476 |
| AcuI | CTGAAG | 2 cut(s) | 1380, 1858 |
| AcyI | GRCGYC | 1 cut(s) | 331 |
| AdeI | CACNNNGTG | 1 cut(s) | 902 |
| AfaI | GTAC | 5 cut(s) | 827, 1457, 1778, 1896, 2609 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 1939, 1987, 2249, 2250, 2418 |
| AflII | CTTAAG | 1 cut(s) | 2636 |
| AflIII | ACRYGT | 1 cut(s) | 846 |
| AhlI | ACTAGT | 2 cut(s) | 1090, 1460 |
| AjiI | CACGTC | 2 cut(s) | 183, 847 |
| AjnI | CCWGG | 1 cut(s) | 2247 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 486, 518, 1894, 1926 |
| AleI | CACNNNNGTG | 1 cut(s) | 365 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 50, 82 |
| Alw21I | GWGCWC | 2 cut(s) | 163, 1582 |
| Alw26I | GTCTC | 5 cut(s) | 173, 577, 682, 2130, 2612 |
| AlwI | GGATC | 2 cut(s) | 601, 1173 |
| AoxI | GGCC | 4 cut(s) | 3, 207, 375, 2379 |
| ApeKI | GCWGC | 4 cut(s) | 11, 1246, 1406, 2175 |
| ApoI | RAATTY | 8 cut(s) | 236, 725, 760, 1053, 1532, 1758, 1908, 2476 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 131, 650, 1031, 1110, 2211 |
| Asp718I | GGTACC | 1 cut(s) | 825 |
| AspLEI | GCGC | 2 cut(s) | 447, 1932 |
| AspS9I | GGNCC | 1 cut(s) | 2337 |
| AsuC2I | CCSGG | 2 cut(s) | 79, 1310 |
| AsuHPI | GGTGA | 4 cut(s) | 619, 2226, 2527, 2714 |
| AsuII | TTCGAA | 1 cut(s) | 1852 |
| AvaII | GGWCC | 1 cut(s) | 2337 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1447, 1480 |
| BanI | GGYRCC | 1 cut(s) | 825 |
| BanII | GRGCYC | 1 cut(s) | 1389 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 1881, 1913, 2343, 2375 |
| BbsI | GAAGAC | 7 cut(s) | 228, 519, 705, 942, 1103, 1140, 1320 |
| Bbv12I | GWGCWC | 2 cut(s) | 163, 1582 |
| BbvCI | CCTCAGC | 2 cut(s) | 7, 147 |
| BbvI | GCAGC | 3 cut(s) | 1233, 1418, 2187 |
| BccI | CCATC | 4 cut(s) | 531, 1544, 1941, 2008 |
| BceAI | ACGGC | 3 cut(s) | 1060, 1569, 2477 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 644, 678 |
| BciT130I | CCWGG | 1 cut(s) | 2249 |
| BciVI | GTATCC | 1 cut(s) | 992 |
| BclI | TGATCA | 3 cut(s) | 717, 1323, 1920 |
| BcnI | CCSGG | 2 cut(s) | 79, 1310 |
| BcoDI | GTCTC | 5 cut(s) | 173, 577, 682, 2130, 2612 |
| BcuI | ACTAGT | 2 cut(s) | 1090, 1460 |
| BfaI | CTAG | 9 cut(s) | 611, 1091, 1394, 1461, 1730, 1817, 2180, 2439, 2600 |
| BfmI | CTRYAG | 4 cut(s) | 363, 730, 778, 2715 |
| BfrI | CTTAAG | 1 cut(s) | 2636 |
| BfuI | GTATCC | 1 cut(s) | 992 |
| BglII | AGATCT | 1 cut(s) | 19 |
| BisI | GCNGC | 4 cut(s) | 12, 1247, 1407, 2176 |
| BlpI | GCTNAGC | 1 cut(s) | 2465 |
| BlsI | GCNGC | 4 cut(s) | 13, 1248, 1408, 2177 |
| Bme1390I | CCNGG | 3 cut(s) | 79, 1310, 2249 |
| Bme18I | GGWCC | 1 cut(s) | 2337 |
| BmgBI | CACGTC | 2 cut(s) | 183, 847 |
| BmgT120I | GGNCC | 1 cut(s) | 2337 |
| BmiI | GGNNCC | 1 cut(s) | 827 |
| BmrFI | CCNGG | 3 cut(s) | 79, 1310, 2249 |
| BmrI | ACTGGG | 1 cut(s) | 1949 |
| BmuI | ACTGGG | 1 cut(s) | 1949 |
| BpiI | GAAGAC | 7 cut(s) | 228, 519, 705, 942, 1103, 1140, 1320 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1798, 1830 |
| BpmI | CTGGAG | 5 cut(s) | 131, 1455, 1596, 2408, 2513 |
| Bpu10I | CCTNAGC | 2 cut(s) | 7, 147 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2465 |
| Bpu14I | TTCGAA | 1 cut(s) | 1852 |
| BpuEI | CTTGAG | 3 cut(s) | 74, 1091, 1975 |
| BpuMI | CCSGG | 2 cut(s) | 79, 1310 |
| BsaAI | YACGTR | 1 cut(s) | 1499 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2667 |
| BsaHI | GRCGYC | 1 cut(s) | 331 |
| BsaI | GGTCTC | 1 cut(s) | 2130 |
| BsaJI | CCNNGG | 4 cut(s) | 78, 576, 2101, 2248 |
| BsaWI | WCCGGW | 1 cut(s) | 1063 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 78, 108 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 1939, 1987, 2249, 2250, 2418 |
| Bse1I | ACTGG | 5 cut(s) | 1579, 1944, 2280, 2382, 2530 |
| Bse3DI | GCAATG | 4 cut(s) | 72, 1217, 2106, 2567 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2667 |
| BseBI | CCWGG | 1 cut(s) | 2249 |
| BseDI | CCNNGG | 4 cut(s) | 78, 576, 2101, 2248 |
| BseGI | GGATG | 6 cut(s) | 65, 542, 751, 847, 859, 2318 |
| BseJI | GATNNNNATC | 1 cut(s) | 2667 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 1939, 1987, 2249, 2250, 2418 |
| BseMI | GCAATG | 4 cut(s) | 72, 1217, 2106, 2567 |
| BseMII | CTCAG | 2 cut(s) | 21, 138 |
| BseNI | ACTGG | 5 cut(s) | 1579, 1944, 2280, 2382, 2530 |
| BseRI | GAGGAG | 2 cut(s) | 191, 2229 |
| BseXI | GCAGC | 3 cut(s) | 1233, 1418, 2187 |
| BshFI | GGCC | 4 cut(s) | 5, 209, 377, 2381 |
| BshNI | GGYRCC | 1 cut(s) | 825 |
| BsiHKAI | GWGCWC | 2 cut(s) | 163, 1582 |
| BsiSI | CCGG | 3 cut(s) | 78, 1064, 1310 |
| BslFI | GGGAC | 2 cut(s) | 436, 2571 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 1939, 1987, 2249, 2250, 2418 |
| BsmAI | GTCTC | 5 cut(s) | 173, 577, 682, 2130, 2612 |
| BsmBI | CGTCTC | 1 cut(s) | 173 |
| BsmFI | GGGAC | 2 cut(s) | 436, 2571 |
| BsmI | GAATGC | 3 cut(s) | 48, 1031, 1747 |
| BsnI | GGCC | 4 cut(s) | 5, 209, 377, 2381 |
| Bso31I | GGTCTC | 1 cut(s) | 2130 |
| Bsp119I | TTCGAA | 1 cut(s) | 1852 |
| Bsp1286I | GDGCHC | 3 cut(s) | 163, 1389, 1582 |
| Bsp143I | GATC | 8 cut(s) | 19, 88, 593, 717, 1165, 1323, 1489, 1920 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2465 |
| BspACI | CCGC | 2 cut(s) | 467, 1409 |
| BspANI | GGCC | 4 cut(s) | 5, 209, 377, 2381 |
| BspCNI | CTCAG | 2 cut(s) | 20, 139 |
| BspHI | TCATGA | 1 cut(s) | 771 |
| BspLI | GGNNCC | 1 cut(s) | 827 |
| BspMAI | CTGCAG | 1 cut(s) | 2719 |
| BspPI | GGATC | 2 cut(s) | 601, 1173 |
| BspT104I | TTCGAA | 1 cut(s) | 1852 |
| BspT107I | GGYRCC | 1 cut(s) | 825 |
| BspTI | CTTAAG | 1 cut(s) | 2636 |
| BspTNI | GGTCTC | 1 cut(s) | 2130 |
| BsrDI | GCAATG | 4 cut(s) | 72, 1217, 2106, 2567 |
| BsrI | ACTGG | 5 cut(s) | 1579, 1944, 2280, 2382, 2530 |
| BssECI | CCNNGG | 4 cut(s) | 78, 576, 2101, 2248 |
| BssMI | GATC | 8 cut(s) | 19, 88, 593, 717, 1165, 1323, 1489, 1920 |
| BssNI | GRCGYC | 1 cut(s) | 331 |
| BssT1I | CCWWGG | 1 cut(s) | 2101 |
| Bst2UI | CCWGG | 1 cut(s) | 2249 |
| Bst4CI | ACNGT | 8 cut(s) | 367, 659, 676, 980, 1603, 1949, 1982, 2623 |
| Bst6I | CTCTTC | 1 cut(s) | 2151 |
| BstACI | GRCGYC | 1 cut(s) | 331 |
| BstAFI | CTTAAG | 1 cut(s) | 2636 |
| BstBAI | YACGTR | 1 cut(s) | 1499 |
| BstBI | TTCGAA | 1 cut(s) | 1852 |
| BstC8I | GCNNGC | 7 cut(s) | 163, 193, 1411, 1506, 1582, 2269, 2471 |
| BstDEI | CTNAG | 8 cut(s) | 7, 147, 707, 1152, 1250, 2075, 2465, 2536 |
| BstDSI | CCRYGG | 1 cut(s) | 576 |
| BstEII | GGTNACC | 1 cut(s) | 1943 |
| BstF5I | GGATG | 6 cut(s) | 65, 542, 751, 847, 859, 2318 |
| BstHHI | GCGC | 2 cut(s) | 447, 1932 |
| BstKTI | GATC | 8 cut(s) | 22, 91, 596, 720, 1168, 1326, 1492, 1923 |
| BstMAI | GTCTC | 5 cut(s) | 173, 577, 682, 2130, 2612 |
| BstMBI | GATC | 8 cut(s) | 19, 88, 593, 717, 1165, 1323, 1489, 1920 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 11, 1127, 2461, 2470, 2714 |
| BstNI | CCWGG | 1 cut(s) | 2249 |
| BstNSI | RCATGY | 1 cut(s) | 1479 |
| BstPI | GGTNACC | 1 cut(s) | 1943 |
| BstSCI | CCNGG | 3 cut(s) | 77, 1308, 2247 |
| BstSFI | CTRYAG | 4 cut(s) | 363, 730, 778, 2715 |
| BstV1I | GCAGC | 3 cut(s) | 1233, 1418, 2187 |
| BstV2I | GAAGAC | 7 cut(s) | 228, 519, 705, 942, 1103, 1140, 1320 |
| BstX2I | RGATCY | 1 cut(s) | 19 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 44 |
| BstYI | RGATCY | 1 cut(s) | 19 |
| BsuI | GTATCC | 1 cut(s) | 992 |
| BsuRI | GGCC | 4 cut(s) | 5, 209, 377, 2381 |
| BtgI | CCRYGG | 1 cut(s) | 576 |
| BtrI | CACGTC | 2 cut(s) | 183, 847 |
| BtsCI | GGATG | 6 cut(s) | 65, 542, 751, 847, 859, 2318 |
| BtsI | GCAGTG | 1 cut(s) | 2724 |
| BtsIMutI | CAGTG | 4 cut(s) | 372, 1665, 1686, 2724 |
| Cac8I | GCNNGC | 7 cut(s) | 163, 193, 1411, 1506, 1582, 2269, 2471 |
| CciI | TCATGA | 1 cut(s) | 771 |
| CfoI | GCGC | 2 cut(s) | 447, 1932 |
| Cfr13I | GGNCC | 1 cut(s) | 2337 |
| Csp6I | GTAC | 5 cut(s) | 826, 1456, 1777, 1895, 2608 |
| CviQI | GTAC | 5 cut(s) | 826, 1456, 1777, 1895, 2608 |
| DdeI | CTNAG | 8 cut(s) | 7, 147, 707, 1152, 1250, 2075, 2465, 2536 |
| DpnI | GATC | 8 cut(s) | 21, 90, 595, 719, 1167, 1325, 1491, 1922 |
| DpnII | GATC | 8 cut(s) | 19, 88, 593, 717, 1165, 1323, 1489, 1920 |
| DraI | TTTAAA | 1 cut(s) | 2451 |
| DraIII | CACNNNGTG | 1 cut(s) | 902 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2382 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2382 |
| Eam1104I | CTCTTC | 1 cut(s) | 2151 |
| EarI | CTCTTC | 1 cut(s) | 2151 |
| Eco130I | CCWWGG | 1 cut(s) | 2101 |
| Eco24I | GRGCYC | 1 cut(s) | 1389 |
| Eco31I | GGTCTC | 1 cut(s) | 2130 |
| Eco47I | GGWCC | 1 cut(s) | 2337 |
| Eco57I | CTGAAG | 2 cut(s) | 1380, 1858 |
| Eco91I | GGTNACC | 1 cut(s) | 1943 |
| EcoO65I | GGTNACC | 1 cut(s) | 1943 |
| EcoRI | GAATTC | 2 cut(s) | 236, 725 |
| EcoRII | CCWGG | 1 cut(s) | 2247 |
| EcoT14I | CCWWGG | 1 cut(s) | 2101 |
| EcoT22I | ATGCAT | 4 cut(s) | 667, 1009, 1488, 2555 |
| EcoT38I | GRGCYC | 1 cut(s) | 1389 |
| ErhI | CCWWGG | 1 cut(s) | 2101 |
| Esp3I | CGTCTC | 1 cut(s) | 173 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 690, 722 |
| FaqI | GGGAC | 2 cut(s) | 436, 2571 |
| FauI | CCCGC | 1 cut(s) | 1402 |
| FauNDI | CATATG | 1 cut(s) | 249 |
| FbaI | TGATCA | 3 cut(s) | 717, 1323, 1920 |
| FblI | GTMKAC | 1 cut(s) | 1182 |
| Fnu4HI | GCNGC | 4 cut(s) | 12, 1247, 1407, 2176 |
| FokI | GGATG | 6 cut(s) | 52, 549, 758, 854, 866, 2305 |
| FriOI | GRGCYC | 1 cut(s) | 1389 |
| Fsp4HI | GCNGC | 4 cut(s) | 12, 1247, 1407, 2176 |
| FspBI | CTAG | 9 cut(s) | 611, 1091, 1394, 1461, 1730, 1817, 2180, 2439, 2600 |
| FspI | TGCGCA | 1 cut(s) | 446 |
| GlaI | GCGC | 2 cut(s) | 446, 1931 |
| GluI | GCNGC | 4 cut(s) | 12, 1247, 1407, 2176 |
| GsuI | CTGGAG | 5 cut(s) | 131, 1455, 1596, 2408, 2513 |
| HaeIII | GGCC | 4 cut(s) | 5, 209, 377, 2381 |
| HapII | CCGG | 3 cut(s) | 78, 1064, 1310 |
| HhaI | GCGC | 2 cut(s) | 447, 1932 |
| Hin1I | GRCGYC | 1 cut(s) | 331 |
| Hin6I | GCGC | 2 cut(s) | 445, 1930 |
| HinP1I | GCGC | 2 cut(s) | 445, 1930 |
| HincII | GTYRAC | 2 cut(s) | 859, 2221 |
| HindII | GTYRAC | 2 cut(s) | 859, 2221 |
| HindIII | AAGCTT | 5 cut(s) | 712, 1847, 2431, 2453, 2467 |
| HpaII | CCGG | 3 cut(s) | 78, 1064, 1310 |
| HphI | GGTGA | 4 cut(s) | 619, 2226, 2527, 2714 |
| Hpy166II | GTNNAC | 7 cut(s) | 805, 859, 1061, 1183, 1301, 1458, 2221 |
| Hpy188I | TCNGA | 4 cut(s) | 1708, 1877, 2005, 2053 |
| Hpy8I | GTNNAC | 7 cut(s) | 805, 859, 1061, 1183, 1301, 1458, 2221 |
| HpyCH4III | ACNGT | 8 cut(s) | 367, 659, 676, 980, 1603, 1949, 1982, 2623 |
| HpyCH4IV | ACGT | 6 cut(s) | 182, 225, 331, 846, 1498, 2683 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 11, 1127, 2461, 2470, 2714 |
| HpyF3I | CTNAG | 8 cut(s) | 7, 147, 707, 1152, 1250, 2075, 2465, 2536 |
| HpySE526I | ACGT | 6 cut(s) | 182, 225, 331, 846, 1498, 2683 |
| Hsp92I | GRCGYC | 1 cut(s) | 331 |
| HspAI | GCGC | 2 cut(s) | 445, 1930 |
| KpnI | GGTACC | 1 cut(s) | 829 |
| Ksp22I | TGATCA | 3 cut(s) | 717, 1323, 1920 |
| Kzo9I | GATC | 8 cut(s) | 19, 88, 593, 717, 1165, 1323, 1489, 1920 |
| LmnI | GCTCC | 7 cut(s) | 112, 158, 1012, 1066, 1554, 1577, 2654 |
| Lsp1109I | GCAGC | 3 cut(s) | 1233, 1418, 2187 |
| MaeI | CTAG | 9 cut(s) | 611, 1091, 1394, 1461, 1730, 1817, 2180, 2439, 2600 |
| MaeII | ACGT | 6 cut(s) | 182, 225, 331, 846, 1498, 2683 |
| MaeIII | GTNAC | 8 cut(s) | 348, 412, 670, 1208, 1635, 1663, 1943, 2191 |
| MalI | GATC | 8 cut(s) | 21, 90, 595, 719, 1167, 1325, 1491, 1922 |
| MboI | GATC | 8 cut(s) | 19, 88, 593, 717, 1165, 1323, 1489, 1920 |
| MfeI | CAATTG | 1 cut(s) | 1749 |
| MflI | RGATCY | 1 cut(s) | 19 |
| MhlI | GDGCHC | 3 cut(s) | 163, 1389, 1582 |
| MlyI | GAGTC | 5 cut(s) | 824, 1462, 2337, 2401, 2651 |
| MmeI | TCCRAC | 5 cut(s) | 64, 484, 1009, 1143, 1445 |
| Mph1103I | ATGCAT | 4 cut(s) | 667, 1009, 1488, 2555 |
| MroXI | GAANNNNTTC | 5 cut(s) | 131, 650, 1031, 1110, 2211 |
| MslI | CAYNNNNRTG | 2 cut(s) | 252, 365 |
| MspA1I | CMGCKG | 2 cut(s) | 11, 1409 |
| MspCI | CTTAAG | 1 cut(s) | 2636 |
| MspI | CCGG | 3 cut(s) | 78, 1064, 1310 |
| MspR9I | CCNGG | 3 cut(s) | 79, 1310, 2249 |
| MunI | CAATTG | 1 cut(s) | 1749 |
| Mva1269I | GAATGC | 3 cut(s) | 48, 1031, 1747 |
| MvaI | CCWGG | 1 cut(s) | 2249 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 11, 1127, 2461, 2470, 2714 |
| NciI | CCSGG | 2 cut(s) | 79, 1310 |
| NdeI | CATATG | 1 cut(s) | 249 |
| NdeII | GATC | 8 cut(s) | 19, 88, 593, 717, 1165, 1323, 1489, 1920 |
| NlaIV | GGNNCC | 1 cut(s) | 827 |
| NmuCI | GTSAC | 2 cut(s) | 1635, 1663 |
| NsbI | TGCGCA | 1 cut(s) | 446 |
| NsiI | ATGCAT | 4 cut(s) | 667, 1009, 1488, 2555 |
| NspI | RCATGY | 1 cut(s) | 1479 |
| NspV | TTCGAA | 1 cut(s) | 1852 |
| OliI | CACNNNNGTG | 1 cut(s) | 365 |
| PagI | TCATGA | 1 cut(s) | 771 |
| PctI | GAATGC | 3 cut(s) | 48, 1031, 1747 |
| PdmI | GAANNNNTTC | 5 cut(s) | 131, 650, 1031, 1110, 2211 |
| PfeI | GAWTC | 5 cut(s) | 470, 912, 1878, 2048, 2167 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1939 |
| PfoI | TCCNGGA | 1 cut(s) | 1308 |
| PkrI | GCNGC | 4 cut(s) | 13, 1248, 1408, 2177 |
| PleI | GAGTC | 5 cut(s) | 823, 1462, 2337, 2400, 2650 |
| PpsI | GAGTC | 5 cut(s) | 823, 1462, 2337, 2400, 2650 |
| Ppu21I | YACGTR | 1 cut(s) | 1499 |
| Psp1406I | AACGTT | 1 cut(s) | 2683 |
| Psp6I | CCWGG | 1 cut(s) | 2247 |
| PspEI | GGTNACC | 1 cut(s) | 1943 |
| PspGI | CCWGG | 1 cut(s) | 2247 |
| PspN4I | GGNNCC | 1 cut(s) | 827 |
| PspPI | GGNCC | 1 cut(s) | 2337 |
| PstI | CTGCAG | 1 cut(s) | 2719 |
| PsuI | RGATCY | 1 cut(s) | 19 |
| PvuII | CAGCTG | 1 cut(s) | 11 |
| RsaI | GTAC | 5 cut(s) | 827, 1457, 1778, 1896, 2609 |
| RsaNI | GTAC | 5 cut(s) | 826, 1456, 1777, 1895, 2608 |
| RseI | CAYNNNNRTG | 2 cut(s) | 252, 365 |
| SatI | GCNGC | 4 cut(s) | 12, 1247, 1407, 2176 |
| Sau3AI | GATC | 8 cut(s) | 19, 88, 593, 717, 1165, 1323, 1489, 1920 |
| Sau96I | GGNCC | 1 cut(s) | 2337 |
| SchI | GAGTC | 5 cut(s) | 824, 1462, 2337, 2401, 2651 |
| ScrFI | CCNGG | 3 cut(s) | 79, 1310, 2249 |
| SduI | GDGCHC | 3 cut(s) | 163, 1389, 1582 |
| SfcI | CTRYAG | 4 cut(s) | 363, 730, 778, 2715 |
| SfuI | TTCGAA | 1 cut(s) | 1852 |
| SinI | GGWCC | 1 cut(s) | 2337 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 252, 365 |
| SmlI | CTYRAG | 4 cut(s) | 53, 1070, 1954, 2636 |
| SmoI | CTYRAG | 4 cut(s) | 53, 1070, 1954, 2636 |
| SpeI | ACTAGT | 2 cut(s) | 1090, 1460 |
| SsiI | CCGC | 2 cut(s) | 467, 1409 |
| SspMI | CTAG | 9 cut(s) | 611, 1091, 1394, 1461, 1730, 1817, 2180, 2439, 2600 |
| StyD4I | CCNGG | 3 cut(s) | 77, 1308, 2247 |
| StyI | CCWWGG | 1 cut(s) | 2101 |
| TaaI | ACNGT | 8 cut(s) | 367, 659, 676, 980, 1603, 1949, 1982, 2623 |
| TaiI | ACGT | 6 cut(s) | 185, 228, 334, 849, 1501, 2686 |
| TaqI | TCGA | 8 cut(s) | 274, 281, 654, 910, 928, 1371, 1852, 2215 |
| TatI | WGTACW | 1 cut(s) | 1776 |
| TfiI | GAWTC | 5 cut(s) | 470, 912, 1878, 2048, 2167 |
| TscAI | CASTG | 4 cut(s) | 372, 1672, 1686, 2724 |
| TseFI | GTSAC | 2 cut(s) | 1635, 1663 |
| TseI | GCWGC | 4 cut(s) | 11, 1246, 1406, 2175 |
| Tsp45I | GTSAC | 2 cut(s) | 1635, 1663 |
| TspGWI | ACGGA | 1 cut(s) | 565 |
| TspRI | CASTG | 4 cut(s) | 372, 1672, 1686, 2724 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1939 |
| Vha464I | CTTAAG | 1 cut(s) | 2636 |
| VpaK11BI | GGWCC | 1 cut(s) | 2337 |
| XapI | RAATTY | 8 cut(s) | 236, 725, 760, 1053, 1532, 1758, 1908, 2476 |
| XbaI | TCTAGA | 1 cut(s) | 1729 |
| XceI | RCATGY | 1 cut(s) | 1479 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 318 |
| XmiI | GTMKAC | 1 cut(s) | 1182 |
| XmnI | GAANNNNTTC | 5 cut(s) | 131, 650, 1031, 1110, 2211 |
| XspI | CTAG | 9 cut(s) | 611, 1091, 1394, 1461, 1730, 1817, 2180, 2439, 2600 |
| ZraI | GACGTC | 1 cut(s) | 332 |
| Zsp2I | ATGCAT | 4 cut(s) | 667, 1009, 1488, 2555 |