Rw5G012000

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000110
Physical Location & Seq
Forward (+)
14014281 .. 14017034
2754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G012000.1

Sequence Viewer

Length: 2754 bp
ATGGCCTCATCTGTAACGGACCTTTTAATTGGAAAACTTGCGACGATTCTTGAGAACGAAGGAACAGCCATAGCTGGTGTTCGTGATGAAGTTGATAAGGTTAAGGAGGAGCTTCAAAGCATGAAAGCATTCCTAGTGGATGTGGAGGCCGACACAGCAAAAACTGAAGGAGAGAAACTGTGGGTTGCAAGAATCAGAGACTTAACCTATGATGTTGAAGATATCATTGATGAATTCATGTATCACATGTATGAGAAGCAAAGTGGGGGTCGACTAACAAGGGGGCTACACAAAACCATTCGCGCTCCACGCAATCTTTGGTTCAGGCATAAAATTGCAAAGAAGTTGCAGAAAATTACTGAAATGATCAAAGCCAATCCAGAGAGGAATCAAAGATACGGTGTTGGCCTTGTAGGAGGAGCATCTACATCTGAAGATATTCACAAATTGGTGCAGAGCCAAGCTGAGTCTTCGTTTTTCATTATGGAAGACGAACTGGTTGGGATTGAAGGCAAGAAGCAAATACTAATGGGATGGCTCATGGATGAAGAGCAACACCAAACTGTTATATCTGTGGTTGGCATGGGAGGTTCTGGAAAGACAACTCTAGTTGCCAAGACCTTCAACAGTGAAAGGGTGAAGAGACATTTCGATTGTTATGCATGGATTACCGTTTCCCAAACTCATAATGTGGCACACTTGTTTCGAAGCTTGATCAAGGAACTCTACAAATCAAGGAAGGAAGAGTGCTGTGCAAATTTGAATGCCATGAGCCGCACAGAATTGATAGAGTTACTAATTAACTACTTGGAGTCCAACAAATACCTGGTTGTACTAGATGACGTGTGGGATATAAAAGTTTGGAGAGAAATAAAGGTATCACTTCAAGATAGGCAACTCAGAAGTCGAATCATAATTACAACTCGAAAAGAAGAGGTAGCAGCCAATTCTTTTGGAGTTAAAAGCCATGTTCACCGTATTGAACCCCTGCTAAAGGATGAGGCTTGGGAGCTCTTCTGCAAAAAATCATTCTCATCTAATGAGCACAAAACTTGTCCACCTGATTTTGTGTCATTAACTTGCCAACTTGTTGAAAAGTGTAGAGGCCTTCCTCTGGCTATTGTGGCTTTAGGTGGTCTTATGTCTTCCAAGAATTCGCTGGATCAATGGCAGCAAGTCTGCAACAGCTTGAACTGGCATCTCGATAACAATTCATTGCTGGACCCCGTGAAAAACATCTTGTTGCTGAGTTTCAATGATTTGCCATCTCAATTGAAACATTGCTTCCTCTATTGTTCTCTTTTCCCAGAAGATTTTGTAATTAAGAGAAAAAGGCTCATTAGATTGTGGATAGCCGAAGGATTTGTTGAAAATGTGAAAGGGATCACGCCCGAAGAAGTTGCAGATGGTTATCTTTGGGAACTTTGCTTCCGTACCATGTTACAAGTTGTTCAGAGAAATGTAACAGGAAGGCCAAAAAAAGTTAAGATGCATGATCTGATGCGAGAGCTTGGTTTGTCGACAGCCGAGAAGGAAAAGTTTGGTCATGTATACGATGGGAGACAAGTAATGGAAGAGATCTCAACCCGTCGCTTGTCAATTCACAAAATGGAAGGAGAAATTAAATCATGGCCGGGTATGTCAAAGATTCGTTCTCTTCTTGTATTTGCAACTGACATGTCCTCATCGTCTTTCTCAAATGCACTGGTTTCTCGATTCAAATTGTTGAGGACTCTAGACTTGGAGGGTGTCCAAATTGATAAACTGCCGGATGCAGTTGTTGACTTGTTCAACTTGAGATATTTAAATTTGACGAACACTTTAATCGAGGAACTTCCAGAGTCCATAGGGCATCTCCGCAACCTTCAATTTTTGGTACTGTGGCGTACTAAGATAGAGGCACTTCCAGAAGGAATTGCAAAATTGCTGAACCTGCGCCATCTAAGTATGTACAGCGACACAGGAGATCGTACGAAATTCAAAATTGCGAGTGGGACAAGAGCGCCATCAAATATTTGTCAGTTGCAGAAATTGCAATGTTTATCAATAATTGAATCAGAAGGGGACACTATTAGACTTATCGGCAACATGACTCAACTCAGGAGCATCGGCATATCGAATGTGAAAGAAACAGATGAGATTGACCTTCGGGTTTCAATTAAGAAGATGACTCTGCTTCAAACTTTGCTTTTAATGGCAAGTAATGAAGAGGAAATCCTTCCGGTGAAAGCACAATTTCCATCTCTTCCGCACCTTAAAACGCTTCAATTGATTGGCAGACTGCAAAACGTAATACCTTGGTTTTCTTCACTGCATAGCCTCACATATCTGTTGCTGCATTGGTCAAGACTTGAAGACGATGTACTACCTCAAATTGAAGCACTGCCTAATCTGACGAGGCTTCGTCTTTGTAATGCATATGTTGGGGAAGAGTTGTGTTTCCGCAGAGGCTTTGTAAAGCTGGAGATATTGAATTTGTGGAATTTTGCCTCGGTGAAAAAGATTACTATAGAGAAAGGGGGGATGCCAAATCTCCAGGTGTTATGGGTTAAACAGTGCATGGAATTGAAGACATTGCCACTGGGCCTTGAATACCTTTCTAATCTACGACGTTTGCGCCTAGATGATGTCCCTGTGGAAGTTATAAAGCCCATTCGGAATGGAGGTGCGGACCGGTCTAAGGTACAACACATCCCCGAGATCTTACATATTTTCGAACAGTCATCCAAGCGGTTCTACGAAAACTTGGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

917

Amino Acids

105.14

Weight (kDa)

8.55

Isoelectric Point (pI)

53.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 88 2.8e-22 Rx N-terminal domain
NB-ARC PF00931 172 - 347 2.2e-47 NB-ARC domain
WHD_DRP PF23559 434 - 504 2.1e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 549 - 874 5e-41 Leucine-rich repeat region
LRR_8 PF13855 576 - 629 8.3e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2645
Acc36I ACCTGC 1 cut(s) 1941
AccI GTMKAC 3 cut(s) 271, 1520, 1551
AccII CGCG 1 cut(s) 303
AciI CCGC 6 cut(s) 775, 1858, 2249, 2443, 2669, 2731
AclWI GGATC 2 cut(s) 1170, 1391
AcoI YGGCCR 1 cut(s) 1631
AcsI RAATTY 7 cut(s) 233, 757, 1153, 1807, 1976, 2473, 2482
AcuI CTGAAG 2 cut(s) 186, 453
AfaI GTAC 8 cut(s) 834, 1435, 1878, 1888, 1952, 1972, 2364, 2685
AfiI CCNNNNNNNGG 3 cut(s) 994, 1114, 2680
AflIII ACRYGT 3 cut(s) 246, 843, 1677
AgeI ACCGGT 1 cut(s) 2673
AjiI CACGTC 1 cut(s) 844
AjnI CCWGG 2 cut(s) 825, 2535
AloI GAACNNNNNNTCC 2 cut(s) 1413, 1445
AluBI AGCT 8 cut(s) 74, 112, 464, 711, 1012, 1188, 1510, 2461
AluI AGCT 8 cut(s) 74, 112, 464, 711, 1012, 1188, 1510, 2461
Alw21I GWGCWC 2 cut(s) 1014, 1047
Alw26I GTCTC 3 cut(s) 192, 637, 1555
AlwI GGATC 2 cut(s) 1170, 1391
Ama87I CYCGRG 1 cut(s) 2696
AoxI GGCC 7 cut(s) 3, 147, 406, 1105, 1472, 1631, 2584
ApeKI GCWGC 3 cut(s) 941, 1171, 2335
ApoI RAATTY 7 cut(s) 233, 757, 1153, 1807, 1976, 2473, 2482
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
AsiGI ACCGGT 1 cut(s) 2673
Asp700I GAANNNNTTC 3 cut(s) 128, 438, 2217
AspLEI GCGC 4 cut(s) 305, 1938, 2005, 2619
AspS9I GGNCC 4 cut(s) 19, 1222, 2584, 2671
AsuC2I CCSGG 1 cut(s) 1635
AsuHPI GGTGA 4 cut(s) 649, 965, 2236, 2506
AsuII TTCGAA 2 cut(s) 706, 2715
AvaI CYCGRG 1 cut(s) 2696
AvaII GGWCC 3 cut(s) 19, 1222, 2671
BanII GRGCYC 1 cut(s) 1014
BarI GAAGNNNNNNTAC 2 cut(s) 2346, 2378
BbsI GAAGAC 5 cut(s) 462, 495, 1137, 2361, 2576
Bbv12I GWGCWC 2 cut(s) 1014, 1047
BbvI GCAGC 3 cut(s) 953, 1183, 2322
BccI CCATC 7 cut(s) 528, 1273, 1400, 1550, 1947, 2014, 2248
BcgI CGANNNNNNTGC 4 cut(s) 641, 675, 1382, 1416
BciT130I CCWGG 2 cut(s) 827, 2537
BclI TGATCA 2 cut(s) 366, 714
BcnI CCSGG 1 cut(s) 1635
BcoDI GTCTC 3 cut(s) 192, 637, 1555
BfaI CTAG 5 cut(s) 134, 608, 836, 1736, 2621
BfmI CTRYAG 1 cut(s) 2508
BfoI RGCGCY 1 cut(s) 2006
BfuAI ACCTGC 1 cut(s) 1941
BglII AGATCT 2 cut(s) 1578, 2700
BisI GCNGC 4 cut(s) 775, 942, 1172, 2336
BlsI GCNGC 4 cut(s) 776, 943, 1173, 2337
Bme1390I CCNGG 3 cut(s) 827, 1635, 2537
Bme18I GGWCC 3 cut(s) 19, 1222, 2671
BmeT110I CYCGRG 1 cut(s) 2696
BmgBI CACGTC 1 cut(s) 844
BmgT120I GGNCC 4 cut(s) 19, 1222, 2584, 2671
BmiI GGNNCC 1 cut(s) 1224
BmrFI CCNGG 3 cut(s) 827, 1635, 2537
BmrI ACTGGG 1 cut(s) 2591
BmsI GCATC 8 cut(s) 431, 1207, 1479, 1491, 1762, 1861, 2115, 2514
BmuI ACTGGG 1 cut(s) 2591
BpiI GAAGAC 5 cut(s) 462, 495, 1137, 2361, 2576
BpmI CTGGAG 2 cut(s) 2483, 2519
Bpu14I TTCGAA 2 cut(s) 706, 2715
BpuEI CTTGAG 2 cut(s) 71, 1816
BpuMI CCSGG 1 cut(s) 1635
BsaBI GATNNNNATC 1 cut(s) 1410
BsaJI CCNNGG 2 cut(s) 2297, 2490
BsaWI WCCGGW 2 cut(s) 2221, 2673
Bsc4I CCNNNNNNNGG 3 cut(s) 994, 1114, 2680
Bse118I RCCGGY 1 cut(s) 2673
Bse1I ACTGG 4 cut(s) 501, 1199, 1710, 2586
Bse3DI GCAATG 4 cut(s) 1214, 1279, 2042, 2573
Bse8I GATNNNNATC 1 cut(s) 1410
BseBI CCWGG 2 cut(s) 827, 2537
BseDI CCNNGG 2 cut(s) 2297, 2490
BseGI GGATG 8 cut(s) 145, 539, 550, 1003, 1777, 2529, 2691, 2723
BseJI GATNNNNATC 1 cut(s) 1410
BseLI CCNNNNNNNGG 3 cut(s) 994, 1114, 2680
BseMI GCAATG 4 cut(s) 1214, 1279, 2042, 2573
BseMII CTCAG 4 cut(s) 456, 913, 1238, 2113
BseNI ACTGG 4 cut(s) 501, 1199, 1710, 2586
BseRI GAGGAG 2 cut(s) 122, 432
BseXI GCAGC 3 cut(s) 953, 1183, 2322
BsgI GTGCAG 1 cut(s) 473
Bsh1236I CGCG 1 cut(s) 303
BshFI GGCC 7 cut(s) 5, 149, 408, 1107, 1474, 1633, 2586
BshTI ACCGGT 1 cut(s) 2673
BsiHKAI GWGCWC 2 cut(s) 1014, 1047
BsiHKCI CYCGRG 1 cut(s) 2696
BsiSI CCGG 4 cut(s) 1634, 1769, 2222, 2674
BsiWI CGTACG 1 cut(s) 1970
BslFI GGGAC 3 cut(s) 2008, 2078, 2615
BslI CCNNNNNNNGG 3 cut(s) 994, 1114, 2680
BsmAI GTCTC 3 cut(s) 192, 637, 1555
BsmFI GGGAC 3 cut(s) 2008, 2078, 2615
BsmI GAATGC 2 cut(s) 128, 769
BsnI GGCC 7 cut(s) 5, 149, 408, 1107, 1474, 1633, 2586
BsoBI CYCGRG 1 cut(s) 2696
Bsp119I TTCGAA 2 cut(s) 706, 2715
Bsp1286I GDGCHC 2 cut(s) 1014, 1047
Bsp1407I TGTACA 1 cut(s) 1950
Bsp143I GATC 8 cut(s) 366, 714, 1162, 1383, 1495, 1578, 1966, 2700
BspACI CCGC 6 cut(s) 775, 1858, 2249, 2443, 2669, 2731
BspANI GGCC 7 cut(s) 5, 149, 408, 1107, 1474, 1633, 2586
BspCNI CTCAG 4 cut(s) 457, 912, 1239, 2112
BspFNI CGCG 1 cut(s) 303
BspLI GGNNCC 1 cut(s) 1224
BspMI ACCTGC 1 cut(s) 1941
BspPI GGATC 2 cut(s) 1170, 1391
BspQI GCTCTTC 2 cut(s) 543, 1019
BspT104I TTCGAA 2 cut(s) 706, 2715
BsrDI GCAATG 4 cut(s) 1214, 1279, 2042, 2573
BsrFI RCCGGY 1 cut(s) 2673
BsrGI TGTACA 1 cut(s) 1950
BsrI ACTGG 4 cut(s) 501, 1199, 1710, 2586
BssAI RCCGGY 1 cut(s) 2673
BssECI CCNNGG 2 cut(s) 2297, 2490
BssMI GATC 8 cut(s) 366, 714, 1162, 1383, 1495, 1578, 1966, 2700
BssNAI GTATAC 1 cut(s) 1552
BssT1I CCWWGG 1 cut(s) 2297
Bst1107I GTATAC 1 cut(s) 1552
Bst2UI CCWGG 2 cut(s) 827, 2537
Bst4CI ACNGT 9 cut(s) 180, 401, 565, 629, 673, 977, 1881, 2556, 2721
BstAPI GCANNNNNTGC 1 cut(s) 2032
BstAUI TGTACA 1 cut(s) 1950
BstBI TTCGAA 2 cut(s) 706, 2715
BstDEI CTNAG 7 cut(s) 465, 899, 1247, 1890, 1943, 2099, 2679
BstENI CCTNNNNNAGG 1 cut(s) 992
BstF5I GGATG 8 cut(s) 145, 539, 550, 1003, 1777, 2529, 2691, 2723
BstFNI CGCG 1 cut(s) 303
BstH2I RGCGCY 1 cut(s) 2006
BstHHI GCGC 4 cut(s) 305, 1938, 2005, 2619
BstKTI GATC 8 cut(s) 369, 717, 1165, 1386, 1498, 1581, 1969, 2703
BstMAI GTCTC 3 cut(s) 192, 637, 1555
BstMBI GATC 8 cut(s) 366, 714, 1162, 1383, 1495, 1578, 1966, 2700
BstMWI GCNNNNNNNGC 5 cut(s) 155, 309, 1124, 1933, 2032
BstNI CCWGG 2 cut(s) 827, 2537
BstNSI RCATGY 2 cut(s) 250, 1681
BstSCI CCNGG 3 cut(s) 825, 1633, 2535
BstSFI CTRYAG 1 cut(s) 2508
BstUI CGCG 1 cut(s) 303
BstV1I GCAGC 3 cut(s) 953, 1183, 2322
BstV2I GAAGAC 5 cut(s) 462, 495, 1137, 2361, 2576
BstX2I RGATCY 2 cut(s) 1578, 2700
BstYI RGATCY 2 cut(s) 1578, 2700
BstZ17I GTATAC 1 cut(s) 1552
BsuRI GGCC 7 cut(s) 5, 149, 408, 1107, 1474, 1633, 2586
BtrI CACGTC 1 cut(s) 844
BtsCI GGATG 8 cut(s) 145, 539, 550, 1003, 1777, 2529, 2691, 2723
BtsI GCAGTG 2 cut(s) 2309, 2381
BtsIMutI CAGTG 6 cut(s) 634, 1703, 2309, 2381, 2561, 2579
BveI ACCTGC 1 cut(s) 1941
CfoI GCGC 4 cut(s) 305, 1938, 2005, 2619
Cfr10I RCCGGY 1 cut(s) 2673
Cfr13I GGNCC 4 cut(s) 19, 1222, 2584, 2671
CpoI CGGWCCG 1 cut(s) 2671
CsiI ACCWGGT 1 cut(s) 825
Csp6I GTAC 8 cut(s) 833, 1434, 1877, 1887, 1951, 1971, 2363, 2684
CspAI ACCGGT 1 cut(s) 2673
CspI CGGWCCG 1 cut(s) 2671
CviQI GTAC 8 cut(s) 833, 1434, 1877, 1887, 1951, 1971, 2363, 2684
DdeI CTNAG 7 cut(s) 465, 899, 1247, 1890, 1943, 2099, 2679
DpnI GATC 8 cut(s) 368, 716, 1164, 1385, 1497, 1580, 1968, 2702
DpnII GATC 8 cut(s) 366, 714, 1162, 1383, 1495, 1578, 1966, 2700
DraI TTTAAA 1 cut(s) 1806
EaeI YGGCCR 1 cut(s) 1631
Ecl136II GAGCTC 1 cut(s) 1012
Eco130I CCWWGG 1 cut(s) 2297
Eco147I AGGCCT 1 cut(s) 1107
Eco24I GRGCYC 1 cut(s) 1014
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 3 cut(s) 19, 1222, 2671
Eco53kI GAGCTC 1 cut(s) 1012
Eco57I CTGAAG 2 cut(s) 186, 453
Eco88I CYCGRG 1 cut(s) 2696
EcoICRI GAGCTC 1 cut(s) 1012
EcoNI CCTNNNNNAGG 1 cut(s) 992
EcoRI GAATTC 2 cut(s) 233, 1153
EcoRII CCWGG 2 cut(s) 825, 2535
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 2297
EcoT22I ATGCAT 3 cut(s) 664, 1494, 2419
EcoT38I GRGCYC 1 cut(s) 1014
ErhI CCWWGG 1 cut(s) 2297
FaqI GGGAC 3 cut(s) 2008, 2078, 2615
FauNDI CATATG 1 cut(s) 2419
FbaI TGATCA 2 cut(s) 366, 714
FblI GTMKAC 3 cut(s) 271, 1520, 1551
Fnu4HI GCNGC 4 cut(s) 775, 942, 1172, 2336
FokI GGATG 8 cut(s) 152, 546, 557, 1010, 1784, 2536, 2678, 2710
FriOI GRGCYC 1 cut(s) 1014
Fsp4HI GCNGC 4 cut(s) 775, 942, 1172, 2336
FspBI CTAG 5 cut(s) 134, 608, 836, 1736, 2621
GlaI GCGC 4 cut(s) 304, 1937, 2004, 2618
GluI GCNGC 4 cut(s) 775, 942, 1172, 2336
GsuI CTGGAG 2 cut(s) 2483, 2519
HaeII RGCGCY 1 cut(s) 2006
HaeIII GGCC 7 cut(s) 5, 149, 408, 1107, 1474, 1633, 2586
HapII CCGG 4 cut(s) 1634, 1769, 2222, 2674
HhaI GCGC 4 cut(s) 305, 1938, 2005, 2619
Hin6I GCGC 4 cut(s) 303, 1936, 2003, 2617
HinP1I GCGC 4 cut(s) 303, 1936, 2003, 2617
HincII GTYRAC 3 cut(s) 272, 1521, 1783
HindII GTYRAC 3 cut(s) 272, 1521, 1783
HindIII AAGCTT 1 cut(s) 709
HpaII CCGG 4 cut(s) 1634, 1769, 2222, 2674
HphI GGTGA 4 cut(s) 649, 965, 2236, 2506
Hpy166II GTNNAC 6 cut(s) 272, 973, 1058, 1521, 1552, 1783
Hpy188I TCNGA 8 cut(s) 197, 433, 902, 1455, 1500, 2059, 2394, 2658
Hpy8I GTNNAC 6 cut(s) 272, 973, 1058, 1521, 1552, 1783
Hpy99I CGWCG 3 cut(s) 46, 1593, 2613
HpyCH4III ACNGT 9 cut(s) 180, 401, 565, 629, 673, 977, 1881, 2556, 2721
HpyCH4IV ACGT 3 cut(s) 843, 2289, 2611
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 309, 1124, 1933, 2032
HpyF3I CTNAG 7 cut(s) 465, 899, 1247, 1890, 1943, 2099, 2679
HpySE526I ACGT 3 cut(s) 843, 2289, 2611
HspAI GCGC 4 cut(s) 303, 1936, 2003, 2617
Ksp22I TGATCA 2 cut(s) 366, 714
Kzo9I GATC 8 cut(s) 366, 714, 1162, 1383, 1495, 1578, 1966, 2700
LguI GCTCTTC 2 cut(s) 543, 1019
LmnI GCTCC 5 cut(s) 109, 310, 419, 1009, 2103
Lsp1109I GCAGC 3 cut(s) 953, 1183, 2322
LweI GCATC 8 cut(s) 431, 1207, 1479, 1491, 1762, 1861, 2115, 2514
MabI ACCWGGT 1 cut(s) 825
MaeI CTAG 5 cut(s) 134, 608, 836, 1736, 2621
MaeII ACGT 3 cut(s) 843, 2289, 2611
MaeIII GTNAC 4 cut(s) 13, 792, 1440, 1462
MalI GATC 8 cut(s) 368, 716, 1164, 1385, 1497, 1580, 1968, 2702
MboI GATC 8 cut(s) 366, 714, 1162, 1383, 1495, 1578, 1966, 2700
MfeI CAATTG 2 cut(s) 1271, 2267
MflI RGATCY 2 cut(s) 1578, 2700
MhlI GDGCHC 2 cut(s) 1014, 1047
MlyI GAGTC 6 cut(s) 476, 821, 1726, 1850, 2086, 2164
MmeI TCCRAC 1 cut(s) 840
Mph1103I ATGCAT 3 cut(s) 664, 1494, 2419
MroXI GAANNNNTTC 3 cut(s) 128, 438, 2217
MslI CAYNNNNRTG 1 cut(s) 249
MspI CCGG 4 cut(s) 1634, 1769, 2222, 2674
MspR9I CCNGG 3 cut(s) 827, 1635, 2537
MunI CAATTG 2 cut(s) 1271, 2267
Mva1269I GAATGC 2 cut(s) 128, 769
MvaI CCWGG 2 cut(s) 827, 2537
MvnI CGCG 1 cut(s) 303
MwoI GCNNNNNNNGC 5 cut(s) 155, 309, 1124, 1933, 2032
NciI CCSGG 1 cut(s) 1635
NdeI CATATG 1 cut(s) 2419
NdeII GATC 8 cut(s) 366, 714, 1162, 1383, 1495, 1578, 1966, 2700
NlaIV GGNNCC 1 cut(s) 1224
NmeAIII GCCGAG 1 cut(s) 1552
NsiI ATGCAT 3 cut(s) 664, 1494, 2419
NspI RCATGY 2 cut(s) 250, 1681
NspV TTCGAA 2 cut(s) 706, 2715
PceI AGGCCT 1 cut(s) 1107
PciI ACATGT 2 cut(s) 246, 1677
PciSI GCTCTTC 2 cut(s) 543, 1019
PctI GAATGC 2 cut(s) 128, 769
PdmI GAANNNNTTC 3 cut(s) 128, 438, 2217
PfeI GAWTC 7 cut(s) 46, 192, 388, 909, 1648, 1716, 2054
Pfl23II CGTACG 1 cut(s) 1970
PinAI ACCGGT 1 cut(s) 2673
PkrI GCNGC 4 cut(s) 776, 943, 1173, 2337
PleI GAGTC 6 cut(s) 475, 820, 1726, 1849, 2086, 2164
PpsI GAGTC 6 cut(s) 475, 820, 1726, 1849, 2086, 2164
PscI ACATGT 2 cut(s) 246, 1677
PsiI TTATAA 1 cut(s) 2645
Psp124BI GAGCTC 1 cut(s) 1014
Psp6I CCWGG 2 cut(s) 825, 2535
PspGI CCWGG 2 cut(s) 825, 2535
PspLI CGTACG 1 cut(s) 1970
PspN4I GGNNCC 1 cut(s) 1224
PspPI GGNCC 4 cut(s) 19, 1222, 2584, 2671
PsuI RGATCY 2 cut(s) 1578, 2700
RsaI GTAC 8 cut(s) 834, 1435, 1878, 1888, 1952, 1972, 2364, 2685
RsaNI GTAC 8 cut(s) 833, 1434, 1877, 1887, 1951, 1971, 2363, 2684
RseI CAYNNNNRTG 1 cut(s) 249
Rsr2I CGGWCCG 1 cut(s) 2671
RsrII CGGWCCG 1 cut(s) 2671
SacI GAGCTC 1 cut(s) 1014
SalI GTCGAC 2 cut(s) 270, 1519
SapI GCTCTTC 2 cut(s) 543, 1019
SatI GCNGC 4 cut(s) 775, 942, 1172, 2336
Sau3AI GATC 8 cut(s) 366, 714, 1162, 1383, 1495, 1578, 1966, 2700
Sau96I GGNCC 4 cut(s) 19, 1222, 2584, 2671
SchI GAGTC 6 cut(s) 476, 821, 1726, 1850, 2086, 2164
ScrFI CCNGG 3 cut(s) 827, 1635, 2537
SduI GDGCHC 2 cut(s) 1014, 1047
SexAI ACCWGGT 1 cut(s) 825
SfaNI GCATC 8 cut(s) 431, 1207, 1479, 1491, 1762, 1861, 2115, 2514
SfcI CTRYAG 1 cut(s) 2508
SfuI TTCGAA 2 cut(s) 706, 2715
SinI GGWCC 3 cut(s) 19, 1222, 2671
SmiI ATTTAAAT 1 cut(s) 1806
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 2 cut(s) 50, 1795
SmoI CTYRAG 2 cut(s) 50, 1795
SseBI AGGCCT 1 cut(s) 1107
SsiI CCGC 6 cut(s) 775, 1858, 2249, 2443, 2669, 2731
SspI AATATT 1 cut(s) 2014
SspMI CTAG 5 cut(s) 134, 608, 836, 1736, 2621
SstI GAGCTC 1 cut(s) 1014
StuI AGGCCT 1 cut(s) 1107
StyD4I CCNGG 3 cut(s) 825, 1633, 2535
StyI CCWWGG 1 cut(s) 2297
SwaI ATTTAAAT 1 cut(s) 1806
TaaI ACNGT 9 cut(s) 180, 401, 565, 629, 673, 977, 1881, 2556, 2721
TaiI ACGT 3 cut(s) 846, 2292, 2614
TatI WGTACW 3 cut(s) 832, 1950, 2362
TauI GCSGC 1 cut(s) 777
TfiI GAWTC 7 cut(s) 46, 192, 388, 909, 1648, 1716, 2054
TscAI CASTG 6 cut(s) 634, 1710, 2316, 2388, 2561, 2586
TseI GCWGC 3 cut(s) 941, 1171, 2335
TspDTI ATGAA 8 cut(s) 102, 137, 226, 246, 469, 561, 1203, 2220
TspGWI ACGGA 2 cut(s) 32, 1421
TspRI CASTG 6 cut(s) 634, 1710, 2316, 2388, 2561, 2586
VpaK11BI GGWCC 3 cut(s) 19, 1222, 2671
XagI CCTNNNNNAGG 1 cut(s) 992
XapI RAATTY 7 cut(s) 233, 757, 1153, 1807, 1976, 2473, 2482
XbaI TCTAGA 1 cut(s) 1735
XceI RCATGY 2 cut(s) 250, 1681
XcmI CCANNNNNNNNNTGG 3 cut(s) 315, 823, 1156
XmiI GTMKAC 3 cut(s) 271, 1520, 1551
XmnI GAANNNNTTC 3 cut(s) 128, 438, 2217
XspI CTAG 5 cut(s) 134, 608, 836, 1736, 2621
Zsp2I ATGCAT 3 cut(s) 664, 1494, 2419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.