Rh5BG353900

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000110
Physical Location & Seq
Reverse (-)
54827884 .. 54830646
2763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG353900.1

Sequence Viewer

Length: 2625 bp
ATGGCCTTGTCTGTAACGGACCTCTTAATTGGAAAACTTGCGACAATTCTTGAGAACGAAGGAACAACTATAGCAGGTGTTCGTCATGAAGTTGATAAGATTAAGGAGGAGTTTCTAAGCATGAAAGCTTTCCTACTGGATGCGGAGGCCAACAAACCAAAAACTGAAGGACAGAAATTGTGGGTTGCAAGAATCAGAGACTTGGCCTATGATGTTGAAGATATCATTGATGAATTCATGTATCACATGTATGAGAAGCAAAGTGGGGGTCGACTTTCAAGGGGGCTACACAATTCCATTCGCGCTCCATGCAACCTTTGGTTCAGGCATAAAATTGCAAAGAAGTTGCGGAAAATTACTGACATGATCAAAGCCATTCCAGAGAGGAATCAAAGATACGGTGTTGGCCTTGTAGGAGGAGCAACTACATCCGAAGATATTCAGAAATTGGTGCAGAACCAAGCGGAGTCTTCGTTTTACATTATGGAAGACGAACTGGTTGGGATTGAAGGCAAGAAGCAAATACTAATGGGATGGCTAATGGATGATGAGCAACACCAAACTGTTATATCTGTGGTGGGCATGGGAGGTTCTGGAAAGACAACTCTAGTTGCCAAGACCTTCACCAATGAATTGGTAAAGAGACATTTTGATTGTTATGCATGGATTACTGTTTCCCAAACTTATGATGTGGTACACTTGTTTAGAAGCTTGATCAGGGAACTCTACAAATCAAGGAAGGAAAATTTCCCTGCAATTCTGAATGCCATGAGCCGCATAGAATTGCTAGAGTTACTAGTTAACTACTTGGAGTCCAAAAAATACCTAGTTGTTCTAGATGACGTGTGGGATATAAAAGTATGGAGAGAAATAAAGGTATCACTTCAAGATAGACAACTTGGAAGTCGAGTCGTACTTACAACTCGAAATGAAGAAGTAGCATCCAACTCTTTTGGAGTTGAAATCTATATTTACCATATTCAGCCCCTGCAAAAGAAGGAGGCTTGGGAACTCTTCAACAGGAAAGCATTCTCAACTAAGCAACACAAAACTTGTCCAACTGAGCTGAAGTCTTTAGCTTGCCAACTTGTGGAGAGGTGTGAAGGCCTTCCTCTCGGTATTGTGGCTTTAGCTGGTCTTATGTCATCTAAGAAGTCGCTGGATCAATGGCAACAAGTCTGCAACAACTTGAATTGGCTGACTAGATTGTGGATAGCAGAAGGATTTGTTGAAAATGTGAAAGGGGTCACTCCTGAAGAAGTTGCAGGTGCCTATCTTCGGGAACTCTGCTTCCGTAGCATGTTACAATTTGCAGATGGCTTTGTTTCCGGATCACCTTTGGGAAGGCTGAGAAAACTTAAGATGCATGATCTGATGCGAGAGCTTGCTTTGTCGACTGCCGAAAAAGAAAAGTTTGGTTATGTATACGACGGGAGAGAAGTAATGGAAGAGATCTCAACCCGTCGCTTATCAATTCATAGAATGAAAGGAGAAATCAAATCATGGAGGGGTATGTCAAAGATTCGTTCTCTTCTTGTATTTGACACTGACATGTTCTCATTGTCTTTCTTAAATACACTGGTTTCTCGATTCAAATTGTTGAGGACTCTAGACTTGGAGGATGTCCAAATTGATAAACTGCCGGCTGCAATTGTTTACATGTTCAACTTGAGATATTTAAATTTGAAGGGCACTTTAATCGAGGAACTTCCGGAGTCCATAAGGCGTCTCCGCAACCTTCAATTTCTGAACATCAGAGATACTAAGATAGAGTCACTTCCACAAGGAATTAAGAGGTTGCTGAACCTGCGGAATCTAATTATGTATCGCTACACTAGGGACCTGTACTTCAAAGCTGCTAGTGGGACAGAAGCGCCATCAAATATTTGTAAGTTGCAGAAATTGCAAAATTTATCAATAATTGAATCAGAAGGGGACACTTGCAGACTTATCGGCAACATGACTCAACTTAAGTACATCGGCATATCCAATGTAAAAGAAAGAGATGAGATTGACCTTTGGGTCTCAATTAAGAAGTTCACTCTGCTTCAATCTCTAGGTTTACTGGCAAGTAATGAAGAGGAAATCCTTCCGGTGAAAGCACAATGTCCACCTCTTCCTCACCTTCGGAAGCTTAATTTGGTTGGCAGACTGCAAAACGTAATACCTTGGTTTTCTTCACTGCATAGCCTCACATCTCTGGTTCTGCATTGGTCAAGACTTGAAGATGATTTACTACCTCAAATTGAAGCACTGTGCAATCTGACAAGGCTTTATCTTTGTAATGCATATGTTGGGGAAGAGTTGTGTTTCCGTAGAGGCTTTGTAAAGCTGATGAGGTTGGAGTTGTTGAATTTTGCCTCGGTGAAAAAGATAACTATAGAAAATGGGGTGATGCCAAATCTCCAGTACTTATGCGTTAACAGTTTCATGGAGTTAAAGACGGTGCCACTGGGCTTTGAATACCTTTCTACTCTACAATATTTGGGCCTGAAAGATGTTCCCATGGAAGTTATTAGGTCCATTCAGAAAGGAGGTGTGGATCACTATAAAGTACAACACATTCCTGAGATCATACATGTTTTTGAACAGTCATCCAAGTGGATCCACGAAAACTTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

874

Amino Acids

100.68

Weight (kDa)

8.81

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 90 1.7e-23 Rx N-terminal domain
NB-ARC PF00931 172 - 347 8.2e-46 NB-ARC domain
WHD_DRP PF23559 399 - 463 5.4e-13 Disease resistance protein Winged helix domain
LRR_14 PF23598 507 - 828 6.8e-38 Leucine-rich repeat region
LRR_8 PF13855 534 - 590 7.2e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 65, 1256
AasI GACNNNNNNGTC 1 cut(s) 2021
Acc36I ACCTGC 3 cut(s) 65, 1256, 1815
AccB1I GGYRCC 2 cut(s) 1268, 2446
AccB7I CCANNNNNTGG 1 cut(s) 1090
AccI GTMKAC 3 cut(s) 271, 1394, 1425
AccII CGCG 1 cut(s) 303
AccIII TCCGGA 2 cut(s) 1328, 1711
AciI CCGC 6 cut(s) 143, 349, 464, 775, 1732, 1810
AclWI GGATC 5 cut(s) 1170, 1339, 2550, 2599, 2612
AcsI RAATTY 5 cut(s) 233, 745, 1681, 1909, 2353
AcuI CTGAAG 3 cut(s) 186, 1088, 1275
AcyI GRCGYC 1 cut(s) 1726
AfaI GTAC 6 cut(s) 696, 915, 1847, 1976, 2411, 2556
AfiI CCNNNNNNNGG 2 cut(s) 1090, 1278
AflII CTTAAG 2 cut(s) 1358, 1970
AflIII ACRYGT 5 cut(s) 246, 843, 1551, 1659, 2578
AhlI ACTAGT 1 cut(s) 796
AjiI CACGTC 1 cut(s) 844
AjuI GAANNNNNNNTTGG 2 cut(s) 2123, 2155
AloI GAACNNNNNNTCC 2 cut(s) 1275, 1307
AluBI AGCT 9 cut(s) 128, 711, 1066, 1079, 1133, 1384, 1856, 2134, 2332
AluI AGCT 9 cut(s) 128, 711, 1066, 1079, 1133, 1384, 1856, 2134, 2332
Alw26I GTCTC 4 cut(s) 192, 637, 1733, 2029
AlwI GGATC 5 cut(s) 1170, 1339, 2550, 2599, 2612
AlwNI CAGNNNCTG 1 cut(s) 988
Aor13HI TCCGGA 2 cut(s) 1328, 1711
AoxI GGCC 7 cut(s) 3, 147, 204, 406, 1105, 2488, 2619
ApeKI GCWGC 2 cut(s) 1646, 1856
ApoI RAATTY 5 cut(s) 233, 745, 1681, 1909, 2353
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2088
AspLEI GCGC 2 cut(s) 305, 1876
AspS9I GGNCC 4 cut(s) 19, 1840, 2488, 2520
AsuHPI GGTGA 6 cut(s) 616, 1326, 2107, 2114, 2377, 2404
AvaII GGWCC 3 cut(s) 19, 1840, 2520
BaeGI GKGCMC 1 cut(s) 1694
BaeI ACNNNNGTAYC 2 cut(s) 686, 719
BamHI GGATCC 1 cut(s) 2604
BanI GGYRCC 2 cut(s) 1268, 2446
BarI GAAGNNNNNNTAC 2 cut(s) 2217, 2249
BbsI GAAGAC 2 cut(s) 462, 495
BbvI GCAGC 2 cut(s) 1633, 1843
BccI CCATC 3 cut(s) 528, 1310, 1885
BcgI CGANNNNNNTGC 4 cut(s) 1681, 1715, 1933, 1967
BclI TGATCA 2 cut(s) 366, 714
BcoDI GTCTC 4 cut(s) 192, 637, 1733, 2029
BcuI ACTAGT 1 cut(s) 796
BfmI CTRYAG 2 cut(s) 69, 2379
BfoI RGCGCY 1 cut(s) 1877
BfrI CTTAAG 2 cut(s) 1358, 1970
BfuAI ACCTGC 3 cut(s) 65, 1256, 1815
BglII AGATCT 1 cut(s) 1452
BisI GCNGC 3 cut(s) 775, 1647, 1857
BlsI GCNGC 3 cut(s) 776, 1648, 1858
BmcAI AGTACT 1 cut(s) 2411
Bme18I GGWCC 3 cut(s) 19, 1840, 2520
BmgBI CACGTC 1 cut(s) 844
BmgT120I GGNCC 4 cut(s) 19, 1840, 2488, 2520
BmiI GGNNCC 4 cut(s) 1270, 1841, 2448, 2606
BmrI ACTGGG 1 cut(s) 2462
BmsI GCATC 5 cut(s) 130, 950, 1353, 1365, 2385
BmuI ACTGGG 1 cut(s) 2462
BpiI GAAGAC 2 cut(s) 462, 495
BpmI CTGGAG 1 cut(s) 2390
BpuEI CTTGAG 2 cut(s) 71, 1690
BsaHI GRCGYC 1 cut(s) 1726
BsaI GGTCTC 1 cut(s) 2029
BsaJI CCNNGG 3 cut(s) 2168, 2361, 2505
BsaWI WCCGGW 3 cut(s) 1328, 1711, 2092
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 2 cut(s) 1090, 1278
Bse118I RCCGGY 1 cut(s) 1642
Bse1I ACTGG 6 cut(s) 141, 501, 1584, 2070, 2407, 2457
BseAI TCCGGA 2 cut(s) 1328, 1711
BseDI CCNNGG 3 cut(s) 2168, 2361, 2505
BseGI GGATG 7 cut(s) 145, 428, 539, 550, 941, 1627, 2594
BseLI CCNNNNNNNGG 2 cut(s) 1090, 1278
BseMII CTCAG 3 cut(s) 1053, 1340, 2559
BseNI ACTGG 6 cut(s) 141, 501, 1584, 2070, 2407, 2457
BseRI GAGGAG 2 cut(s) 122, 432
BseSI GKGCMC 1 cut(s) 1694
BseXI GCAGC 2 cut(s) 1633, 1843
BsgI GTGCAG 1 cut(s) 473
Bsh1236I CGCG 1 cut(s) 303
BshFI GGCC 7 cut(s) 5, 149, 206, 408, 1107, 2490, 2621
BshNI GGYRCC 2 cut(s) 1268, 2446
BsiSI CCGG 4 cut(s) 1329, 1643, 1712, 2093
BslFI GGGAC 3 cut(s) 1853, 1879, 1949
BslI CCNNNNNNNGG 2 cut(s) 1090, 1278
BsmAI GTCTC 4 cut(s) 192, 637, 1733, 2029
BsmBI CGTCTC 1 cut(s) 1733
BsmFI GGGAC 3 cut(s) 1853, 1879, 1949
BsmI GAATGC 2 cut(s) 769, 1028
BsnI GGCC 7 cut(s) 5, 149, 206, 408, 1107, 2490, 2621
Bso31I GGTCTC 1 cut(s) 2029
Bsp1286I GDGCHC 1 cut(s) 1694
Bsp13I TCCGGA 2 cut(s) 1328, 1711
Bsp143I GATC 9 cut(s) 366, 714, 1162, 1331, 1369, 1452, 2542, 2571, 2604
Bsp19I CCATGG 1 cut(s) 2505
BspACI CCGC 6 cut(s) 143, 349, 464, 775, 1732, 1810
BspANI GGCC 7 cut(s) 5, 149, 206, 408, 1107, 2490, 2621
BspCNI CTCAG 3 cut(s) 1054, 1341, 2560
BspEI TCCGGA 2 cut(s) 1328, 1711
BspFNI CGCG 1 cut(s) 303
BspHI TCATGA 1 cut(s) 85
BspLI GGNNCC 4 cut(s) 1270, 1841, 2448, 2606
BspMI ACCTGC 3 cut(s) 65, 1256, 1815
BspPI GGATC 5 cut(s) 1170, 1339, 2550, 2599, 2612
BspT107I GGYRCC 2 cut(s) 1268, 2446
BspTI CTTAAG 2 cut(s) 1358, 1970
BspTNI GGTCTC 1 cut(s) 2029
BsrFI RCCGGY 1 cut(s) 1642
BsrI ACTGG 6 cut(s) 141, 501, 1584, 2070, 2407, 2457
BssAI RCCGGY 1 cut(s) 1642
BssECI CCNNGG 3 cut(s) 2168, 2361, 2505
BssMI GATC 9 cut(s) 366, 714, 1162, 1331, 1369, 1452, 2542, 2571, 2604
BssNAI GTATAC 1 cut(s) 1426
BssNI GRCGYC 1 cut(s) 1726
BssT1I CCWWGG 2 cut(s) 2168, 2505
Bst1107I GTATAC 1 cut(s) 1426
Bst4CI ACNGT 7 cut(s) 401, 565, 673, 2256, 2426, 2446, 2592
Bst6I CTCTTC 6 cut(s) 1019, 1443, 1536, 2073, 2121, 2295
BstACI GRCGYC 1 cut(s) 1726
BstAFI CTTAAG 2 cut(s) 1358, 1970
BstAPI GCANNNNNTGC 1 cut(s) 1903
BstC8I GCNNGC 3 cut(s) 1081, 1386, 1644
BstDEI CTNAG 7 cut(s) 116, 1038, 1062, 1149, 1349, 1764, 2568
BstDSI CCRYGG 1 cut(s) 2505
BstF5I GGATG 7 cut(s) 145, 428, 539, 550, 941, 1627, 2594
BstFNI CGCG 1 cut(s) 303
BstH2I RGCGCY 1 cut(s) 1877
BstHHI GCGC 2 cut(s) 305, 1876
BstKTI GATC 9 cut(s) 369, 717, 1165, 1334, 1372, 1455, 2545, 2574, 2607
BstMAI GTCTC 4 cut(s) 192, 637, 1733, 2029
BstMBI GATC 9 cut(s) 366, 714, 1162, 1331, 1369, 1452, 2542, 2571, 2604
BstMWI GCNNNNNNNGC 4 cut(s) 309, 1296, 1807, 1903
BstNSI RCATGY 5 cut(s) 250, 1303, 1555, 1663, 2582
BstSFI CTRYAG 2 cut(s) 69, 2379
BstSLI GKGCMC 1 cut(s) 1694
BstUI CGCG 1 cut(s) 303
BstV1I GCAGC 2 cut(s) 1633, 1843
BstV2I GAAGAC 2 cut(s) 462, 495
BstX2I RGATCY 2 cut(s) 1452, 2604
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 2 cut(s) 1452, 2604
BstZ17I GTATAC 1 cut(s) 1426
BsuRI GGCC 7 cut(s) 5, 149, 206, 408, 1107, 2490, 2621
BtgI CCRYGG 1 cut(s) 2505
BtrI CACGTC 1 cut(s) 844
BtsCI GGATG 7 cut(s) 145, 428, 539, 550, 941, 1627, 2594
BtsI GCAGTG 1 cut(s) 2180
BtsIMutI CAGTG 5 cut(s) 1545, 1577, 2180, 2252, 2450
BveI ACCTGC 3 cut(s) 65, 1256, 1815
Cac8I GCNNGC 3 cut(s) 1081, 1386, 1644
CaiI CAGNNNCTG 1 cut(s) 988
CciI TCATGA 1 cut(s) 85
CfoI GCGC 2 cut(s) 305, 1876
Cfr10I RCCGGY 1 cut(s) 1642
Cfr13I GGNCC 4 cut(s) 19, 1840, 2488, 2520
CseI GACGC 1 cut(s) 1715
Csp6I GTAC 6 cut(s) 695, 914, 1846, 1975, 2410, 2555
CviQI GTAC 6 cut(s) 695, 914, 1846, 1975, 2410, 2555
DdeI CTNAG 7 cut(s) 116, 1038, 1062, 1149, 1349, 1764, 2568
DpnI GATC 9 cut(s) 368, 716, 1164, 1333, 1371, 1454, 2544, 2573, 2606
DpnII GATC 9 cut(s) 366, 714, 1162, 1331, 1369, 1452, 2542, 2571, 2604
DraI TTTAAA 1 cut(s) 1680
DrdI GACNNNNNNGTC 1 cut(s) 2021
DseDI GACNNNNNNGTC 1 cut(s) 2021
Eam1104I CTCTTC 6 cut(s) 1019, 1443, 1536, 2073, 2121, 2295
EarI CTCTTC 6 cut(s) 1019, 1443, 1536, 2073, 2121, 2295
Eco130I CCWWGG 2 cut(s) 2168, 2505
Eco147I AGGCCT 1 cut(s) 1107
Eco31I GGTCTC 1 cut(s) 2029
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 3 cut(s) 19, 1840, 2520
Eco57I CTGAAG 3 cut(s) 186, 1088, 1275
EcoO109I RGGNCCY 1 cut(s) 1840
EcoRI GAATTC 1 cut(s) 233
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 2 cut(s) 2168, 2505
EcoT22I ATGCAT 3 cut(s) 664, 1368, 2290
ErhI CCWWGG 2 cut(s) 2168, 2505
Esp3I CGTCTC 1 cut(s) 1733
FaqI GGGAC 3 cut(s) 1853, 1879, 1949
FauNDI CATATG 1 cut(s) 2290
FbaI TGATCA 2 cut(s) 366, 714
FblI GTMKAC 3 cut(s) 271, 1394, 1425
Fnu4HI GCNGC 3 cut(s) 775, 1647, 1857
FokI GGATG 7 cut(s) 152, 415, 546, 557, 928, 1634, 2581
Fsp4HI GCNGC 3 cut(s) 775, 1647, 1857
GlaI GCGC 2 cut(s) 304, 1875
GluI GCNGC 3 cut(s) 775, 1647, 1857
GsuI CTGGAG 1 cut(s) 2390
HaeII RGCGCY 1 cut(s) 1877
HaeIII GGCC 7 cut(s) 5, 149, 206, 408, 1107, 2490, 2621
HapII CCGG 4 cut(s) 1329, 1643, 1712, 2093
HgaI GACGC 1 cut(s) 1715
HhaI GCGC 2 cut(s) 305, 1876
Hin1I GRCGYC 1 cut(s) 1726
Hin6I GCGC 2 cut(s) 303, 1874
HinP1I GCGC 2 cut(s) 303, 1874
HincII GTYRAC 4 cut(s) 272, 802, 1395, 2422
HindII GTYRAC 4 cut(s) 272, 802, 1395, 2422
HindIII AAGCTT 3 cut(s) 126, 709, 2132
HpaI GTTAAC 2 cut(s) 802, 2422
HpaII CCGG 4 cut(s) 1329, 1643, 1712, 2093
HphI GGTGA 6 cut(s) 616, 1326, 2107, 2114, 2377, 2404
Hpy99I CGWCG 2 cut(s) 1433, 1467
HpyCH4III ACNGT 7 cut(s) 401, 565, 673, 2256, 2426, 2446, 2592
HpyCH4IV ACGT 2 cut(s) 843, 2160
HpyF10VI GCNNNNNNNGC 4 cut(s) 309, 1296, 1807, 1903
HpyF3I CTNAG 7 cut(s) 116, 1038, 1062, 1149, 1349, 1764, 2568
HpySE526I ACGT 2 cut(s) 843, 2160
Hsp92I GRCGYC 1 cut(s) 1726
HspAI GCGC 2 cut(s) 303, 1874
Kpn2I TCCGGA 2 cut(s) 1328, 1711
KroI GCCGGC 1 cut(s) 1642
KroNI GCCGGC 1 cut(s) 1644
Ksp22I TGATCA 2 cut(s) 366, 714
KspAI GTTAAC 2 cut(s) 802, 2422
Kzo9I GATC 9 cut(s) 366, 714, 1162, 1331, 1369, 1452, 2542, 2571, 2604
LmnI GCTCC 2 cut(s) 310, 419
Lsp1109I GCAGC 2 cut(s) 1633, 1843
LweI GCATC 5 cut(s) 130, 950, 1353, 1365, 2385
MaeII ACGT 2 cut(s) 843, 2160
MaeIII GTNAC 5 cut(s) 13, 792, 1246, 1302, 1773
MalI GATC 9 cut(s) 368, 716, 1164, 1333, 1371, 1454, 2544, 2573, 2606
MboI GATC 9 cut(s) 366, 714, 1162, 1331, 1369, 1452, 2542, 2571, 2604
MfeI CAATTG 1 cut(s) 1650
MflI RGATCY 2 cut(s) 1452, 2604
MhlI GDGCHC 1 cut(s) 1694
MlyI GAGTC 7 cut(s) 476, 821, 918, 1600, 1724, 1781, 1957
MmeI TCCRAC 3 cut(s) 969, 1082, 2322
Mph1103I ATGCAT 3 cut(s) 664, 1368, 2290
MroI TCCGGA 2 cut(s) 1328, 1711
MroNI GCCGGC 1 cut(s) 1642
MroXI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2088
MslI CAYNNNNRTG 3 cut(s) 249, 1550, 2599
MspCI CTTAAG 2 cut(s) 1358, 1970
MspI CCGG 4 cut(s) 1329, 1643, 1712, 2093
MunI CAATTG 1 cut(s) 1650
Mva1269I GAATGC 2 cut(s) 769, 1028
MvnI CGCG 1 cut(s) 303
MwoI GCNNNNNNNGC 4 cut(s) 309, 1296, 1807, 1903
NaeI GCCGGC 1 cut(s) 1644
NcoI CCATGG 1 cut(s) 2505
NdeI CATATG 1 cut(s) 2290
NdeII GATC 9 cut(s) 366, 714, 1162, 1331, 1369, 1452, 2542, 2571, 2604
NgoMIV GCCGGC 1 cut(s) 1642
NlaIV GGNNCC 4 cut(s) 1270, 1841, 2448, 2606
NmuCI GTSAC 2 cut(s) 1246, 1773
NsiI ATGCAT 3 cut(s) 664, 1368, 2290
NspI RCATGY 5 cut(s) 250, 1303, 1555, 1663, 2582
PagI TCATGA 1 cut(s) 85
PaqCI CACCTGC 2 cut(s) 65, 1256
PceI AGGCCT 1 cut(s) 1107
PciI ACATGT 4 cut(s) 246, 1551, 1659, 2578
PctI GAATGC 2 cut(s) 769, 1028
PdiI GCCGGC 1 cut(s) 1644
PdmI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2088
PfeI GAWTC 6 cut(s) 192, 388, 1522, 1590, 1813, 1925
PflMI CCANNNNNTGG 1 cut(s) 1090
PkrI GCNGC 3 cut(s) 776, 1648, 1858
PleI GAGTC 7 cut(s) 475, 820, 917, 1600, 1723, 1780, 1957
PpsI GAGTC 7 cut(s) 475, 820, 917, 1600, 1723, 1780, 1957
PpuMI RGGWCCY 1 cut(s) 1840
PscI ACATGT 4 cut(s) 246, 1551, 1659, 2578
Psp5II RGGWCCY 1 cut(s) 1840
PspN4I GGNNCC 4 cut(s) 1270, 1841, 2448, 2606
PspPI GGNCC 4 cut(s) 19, 1840, 2488, 2520
PspPPI RGGWCCY 1 cut(s) 1840
PstNI CAGNNNCTG 1 cut(s) 988
PsuI RGATCY 2 cut(s) 1452, 2604
RsaI GTAC 6 cut(s) 696, 915, 1847, 1976, 2411, 2556
RsaNI GTAC 6 cut(s) 695, 914, 1846, 1975, 2410, 2555
RseI CAYNNNNRTG 3 cut(s) 249, 1550, 2599
SalI GTCGAC 2 cut(s) 270, 1393
SatI GCNGC 3 cut(s) 775, 1647, 1857
Sau3AI GATC 9 cut(s) 366, 714, 1162, 1331, 1369, 1452, 2542, 2571, 2604
Sau96I GGNCC 4 cut(s) 19, 1840, 2488, 2520
ScaI AGTACT 1 cut(s) 2411
SchI GAGTC 7 cut(s) 476, 821, 918, 1600, 1724, 1781, 1957
SduI GDGCHC 1 cut(s) 1694
SfaNI GCATC 5 cut(s) 130, 950, 1353, 1365, 2385
SfcI CTRYAG 2 cut(s) 69, 2379
SinI GGWCC 3 cut(s) 19, 1840, 2520
SmiI ATTTAAAT 1 cut(s) 1680
SmiMI CAYNNNNRTG 3 cut(s) 249, 1550, 2599
SmlI CTYRAG 4 cut(s) 50, 1358, 1669, 1970
SmoI CTYRAG 4 cut(s) 50, 1358, 1669, 1970
SpeI ACTAGT 1 cut(s) 796
SseBI AGGCCT 1 cut(s) 1107
SsiI CCGC 6 cut(s) 143, 349, 464, 775, 1732, 1810
SspI AATATT 2 cut(s) 1885, 2483
StuI AGGCCT 1 cut(s) 1107
StyI CCWWGG 2 cut(s) 2168, 2505
SwaI ATTTAAAT 1 cut(s) 1680
TaaI ACNGT 7 cut(s) 401, 565, 673, 2256, 2426, 2446, 2592
TaiI ACGT 2 cut(s) 846, 2163
TaqI TCGA 6 cut(s) 271, 907, 925, 1394, 1588, 1701
TatI WGTACW 4 cut(s) 1845, 1974, 2409, 2554
TauI GCSGC 1 cut(s) 777
TfiI GAWTC 6 cut(s) 192, 388, 1522, 1590, 1813, 1925
TscAI CASTG 5 cut(s) 1552, 1584, 2187, 2259, 2457
TseFI GTSAC 2 cut(s) 1246, 1773
TseI GCWGC 2 cut(s) 1646, 1856
Tsp45I GTSAC 2 cut(s) 1246, 1773
TspGWI ACGGA 3 cut(s) 32, 1283, 2303
TspRI CASTG 5 cut(s) 1552, 1584, 2187, 2259, 2457
Van91I CCANNNNNTGG 1 cut(s) 1090
Vha464I CTTAAG 2 cut(s) 1358, 1970
VpaK11BI GGWCC 3 cut(s) 19, 1840, 2520
XapI RAATTY 5 cut(s) 233, 745, 1681, 1909, 2353
XbaI TCTAGA 2 cut(s) 835, 1609
XceI RCATGY 5 cut(s) 250, 1303, 1555, 1663, 2582
XcmI CCANNNNNNNNNTGG 1 cut(s) 315
XmiI GTMKAC 3 cut(s) 271, 1394, 1425
XmnI GAANNNNTTC 5 cut(s) 128, 438, 1028, 1107, 2088
ZrmI AGTACT 1 cut(s) 2411
Zsp2I ATGCAT 3 cut(s) 664, 1368, 2290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.