Rmu_sc0001289.1_g000014

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001289.1
OG0000110
Physical Location & Seq
Reverse (-)
61788 .. 66932
5145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001289.1_g000014.1.cds

Sequence Viewer

Length: 3564 bp
atggcttcacctgcaatagaccttctgatcggcaaaatagtatcatttcttgaaagtgaagtctcattgctagaaggagttcatgatgagctacacgaagtcaagcttgagttattaaccatgaaagccttcttggaggatgcagaaaggaagggagctctgagtgaagtagagaaaacatgggtggcaaatgtaagagacgtctccattgatgtcgaagatatcattgatgagtttacgtacgacacaaacaagcgaagaagttggggtccgttcacaagagccttttggaagacgctttgcctcccacagaacatctgggagaggcatcggattgccaccaaattgcagacgatcataaaaacaatcaaagatattccagagagaaaccggcgttatggttctgtgcgcatagaaggcagtaattcccaccatgatgtgaaccggattaaagtctatggcgagtcgtctcttttcttgaaagatgatgatctggtggggatcacagatgctagaaggaagttagttggatggttgttgtctagccattctcaaagaactgtgatttcagttgtaggaatggggggttcgggcaagacgaccttagttgcaagcaccttcaacaatcaaactgtacagaaacattttggttgctacacgtggattacagtctcacaaagttataccgttgaagatctgtttagagtcatgatcaatgaattattggcagcggcacaaatggatgttcctcagaacttgagcaacatgagttacagacatttggtggaaatgctagtccactacttgcagccacggaggtatatgattgtcttagatgatgtgtgggacactaatctttggagatccatagatgttgcacttcccaatggaactcatggtagccggatcatgcttacaactcggaacaaagacatagcttctttggcatttggagtcgaaagtcatgttcatcaggttgaacctcttaatacaaatgaggcttgggatcttttctccagaagagcattctctggtgggagtgacaagtcttgtccaccagagcttcaaatcatagctagggagcttgtaggaaaatgtgaaggcttacctctaggacttgtggctttgggttctctcatgtcaaccaaaagatttgtgtccgagtggaagaaagtttacaatagcatgagttgggagctcagcaataatccgacacttgaagtagtgaagagcattttgttgctaagcttcaatgatctaccttaccgactcaaacactgcttcttatatttctgcatttttcctgaagattacacaattaagtgcaaaaggctagttcggttgtggatagcagaggggtttgttgaacaagttagagggggtcagccagaagagatagctgagagctacctagcagagctaacttgccgctgtatgcttcaagtggttgagagggaaccatctggaaaggcaaaaaaatttaaaatgcatgaccttctgcgagaacttgccatttcaatttcagaagcagagaagttttgtactgtgtataatgagaaagaaacaaatgaagagagacgagctccacgccgcctttcgatacaaggaagctctggaaaagtacgagcacacaaagacatgacaaaagtacgctcattatttgtctttgctcctaagatgatggcctcttcttcttctttggaaaaagtattaccatctggtttcaaactgttgagggtcttggatcttaaatatgttccgatccaggaattgccagatgaaataatgaagtgctttaacttgaagagtttgaacttgaaagggacagaagtaagaaagctaccaaaacgcatagggaatcttcagaacttggaaacaatggatattagggactccaatataagggaacttccagttggaattgtgaagttacagaagttgcgacatcttttcatgtaccatttcaattatggagcttacaagtcttttaatagctttcaaggcacaaaagcaccagcaggcatatgtaagctacagagtctgcaaattttggattcaatcgaggcaggaagtgagttgatcaaacagcttcagcacatgacccaactcactgcattaggcttagcgaatgtgaaggaagccgatgagatagacttatgcaagtcaatcgaaagcatgaaactgatggaatgcttaaccgtcaagacaagtagtgaggatgaggttttacgcatggatgcattgttgtcggctcctcctctgctgaaaacacttgttttaactggaaagttaaacaaggttccgctctggtttgcttctcttgagagccttaccgtcttatatttgcattggtccagatcaacagaagactttcttcctcacattcatgcactacctaatttgaccaaactttggctgtgcaatgcatatgtaggaaaccagttgctctttcaaactgggttccaaaagctttctggtttgtacataaccaattttcctcagttgaatctgatcaccatagagaagggggtgatgccagcactgaagactctggacatttctgaatgcatggagttaaagcgattgcctcgcggaatcaaacttctgacatgtctccagaagttgcatctgcacaggggacctcagcagacccaccttcggaccctggctaaaacttggcttgttaatgtggaaaagattgatggcaagttcttcttccgaaagggttggaagaaatttgtgaatggaaatggtctgaagcttggtgaatttcttgtttttcgatattttggaaactcgaaattctttgttgacatatatggaagaaatacctgcaaaaaggattttgtaaaggccgttgagaaaagtgagaaagagggacatggcaatgaaggttaccagggcttaaaaggtgtagctgaaggaactaggactggtcaagccacgaacagtattcacatcaaagatgaagtaatcgatactgaatcggaaagatcgttgcatccattgaccagaaatactggaaaagatgttgctcttgagagagcaaacgctttcaaaattgaagatgaagaaaaacaaggaaatgatgatgaggacgaggatgatgatgattctataagaatcccaaatgaccttccaccatgcacaaaaacaatggagaaatctccattacgagcacaacttgtcaagagaaggcaatcaagttctagtactaacactggtggaaaagatgtagcacttcagaaagccaatgctttcaaatctgcagacccttctttcgtggttgcaatgcatatctcgtatgtccacgcaggttatatgaatgtgccatctgcttttgccaagggatatcttactaagcggcctagtgaagtcaccctccaggttaatggggaaacttggtctgtcaatttacaatatgaaagaaacatgaaaagactcaccacaggttgggcggattttgtgcgagccaataagttggaagttggtaacgcgtgtgtttttgtgttgagtaactacgacaagtttatctttgatgttatcgtttaccgcgccaaatag

Protein Analysis

1187

Amino Acids

134.97

Weight (kDa)

8.87

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 19
AatII GACGTC 1 cut(s) 204
Acc16I TGCGCA 1 cut(s) 410
Acc36I ACCTGC 3 cut(s) 19, 2840, 3305
AccB7I CCANNNNNTGG 2 cut(s) 2412, 3453
AccBSI CCGCTC 1 cut(s) 2305
AccII CGCG 3 cut(s) 2592, 3497, 3555
AciI CCGC 8 cut(s) 731, 1429, 1591, 2303, 2592, 3364, 3458, 3553
AclWI GGATC 6 cut(s) 509, 858, 914, 1014, 1752, 1756
AcsI RAATTY 5 cut(s) 1478, 2046, 2735, 2768, 2801
AcuI CTGAAG 7 cut(s) 1326, 1847, 2075, 2564, 2777, 2940, 3227
AcvI CACGTG 1 cut(s) 660
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 8 cut(s) 242, 636, 1543, 1623, 1650, 1958, 2483, 3214
AfiI CCNNNNNNNGG 4 cut(s) 1902, 2412, 2658, 3453
AflIII ACRYGT 3 cut(s) 657, 2609, 3495
AjnI CCWGG 4 cut(s) 1764, 2664, 2898, 3384
AjuI GAANNNNNNNTTGG 4 cut(s) 116, 148, 1899, 1931
Alw21I GWGCWC 5 cut(s) 160, 1200, 1585, 1630, 3181
Alw26I GTCTC 7 cut(s) 67, 192, 208, 474, 676, 1570, 2618
AlwI GGATC 6 cut(s) 509, 858, 914, 1014, 1752, 1756
AlwNI CAGNNNCTG 1 cut(s) 2041
AoxI GGCC 3 cut(s) 1683, 2853, 3365
ApeKI GCWGC 2 cut(s) 728, 808
ApoI RAATTY 5 cut(s) 1478, 2046, 2735, 2768, 2801
ArsI GACNNNNNNTTYG 2 cut(s) 3263, 3295
Asp700I GAANNNNTTC 3 cut(s) 78, 128, 2370
AspLEI GCGC 2 cut(s) 411, 3557
AspS9I GGNCC 4 cut(s) 269, 2352, 2639, 2661
AsuHPI GGTGA 5 cut(s) 2506, 2542, 2777, 3370, 3436
AvaII GGWCC 4 cut(s) 269, 2352, 2639, 2661
BanII GRGCYC 3 cut(s) 160, 1200, 1585
BarI GAAGNNNNNNTAC 2 cut(s) 1160, 1192
BbrPI CACGTG 1 cut(s) 660
BbsI GAAGAC 3 cut(s) 299, 2373, 2552
Bbv12I GWGCWC 5 cut(s) 160, 1200, 1585, 1630, 3181
BbvCI CCTCAGC 1 cut(s) 2643
BbvI GCAGC 2 cut(s) 740, 820
BccI CCATC 7 cut(s) 525, 1468, 1675, 1723, 2179, 2696, 3340
BceAI ACGGC 1 cut(s) 2840
BcgI CGANNNNNNTGC 4 cut(s) 2149, 2183, 2229, 2263
BciT130I CCWGG 4 cut(s) 1766, 2666, 2900, 3386
BclI TGATCA 3 cut(s) 711, 2079, 2511
BcoDI GTCTC 7 cut(s) 67, 192, 208, 474, 676, 1570, 2618
BfmI CTRYAG 2 cut(s) 2033, 3267
BfuAI ACCTGC 3 cut(s) 19, 2840, 3305
BglII AGATCT 1 cut(s) 694
BisI GCNGC 6 cut(s) 729, 732, 809, 1429, 1591, 3365
BlpI GCTNAGC 3 cut(s) 1199, 1244, 2122
BlsI GCNGC 6 cut(s) 730, 733, 810, 1430, 1592, 3366
BmcAI AGTACT 1 cut(s) 3214
Bme1390I CCNGG 4 cut(s) 1766, 2666, 2900, 3386
Bme18I GGWCC 4 cut(s) 269, 2352, 2639, 2661
BmgT120I GGNCC 4 cut(s) 269, 2352, 2639, 2661
BmiI GGNNCC 7 cut(s) 270, 1458, 2253, 2301, 2462, 2640, 2663
BmrFI CCNGG 4 cut(s) 1766, 2666, 2900, 3386
BmrI ACTGGG 1 cut(s) 2466
BmsI GCATC 7 cut(s) 130, 337, 499, 2227, 2523, 2635, 3010
BmuI ACTGGG 1 cut(s) 2466
BpiI GAAGAC 3 cut(s) 299, 2373, 2552
BplI GAGNNNNNCTC 2 cut(s) 1567, 1599
BpmI CTGGAG 3 cut(s) 1000, 2600, 3368
Bpu10I CCTNAGC 1 cut(s) 2643
Bpu1102I GCTNAGC 3 cut(s) 1199, 1244, 2122
BpuEI CTTGAG 4 cut(s) 128, 778, 2342, 3059
Bsa29I ATCGAT 1 cut(s) 2976
BsaAI YACGTR 2 cut(s) 240, 660
BsaBI GATNNNNATC 1 cut(s) 489
BsaHI GRCGYC 1 cut(s) 201
BsaJI CCNNGG 4 cut(s) 812, 2664, 2899, 3345
BsaWI WCCGGW 1 cut(s) 444
Bsc4I CCNNNNNNNGG 4 cut(s) 1902, 2412, 2658, 3453
Bse118I RCCGGY 1 cut(s) 390
Bse1I ACTGG 7 cut(s) 1913, 2287, 2440, 2461, 2938, 3025, 3226
Bse3DI GCAATG 4 cut(s) 65, 2428, 2893, 3297
Bse8I GATNNNNATC 1 cut(s) 489
BseBI CCWGG 4 cut(s) 1766, 2666, 2900, 3386
BseCI ATCGAT 1 cut(s) 2976
BseDI CCNNGG 4 cut(s) 812, 2664, 2899, 3345
BseGI GGATG 7 cut(s) 145, 536, 748, 2224, 2242, 3001, 3109
BseJI GATNNNNATC 1 cut(s) 489
BseLI CCNNNNNNNGG 4 cut(s) 1902, 2412, 2658, 3453
BseMI GCAATG 4 cut(s) 65, 2428, 2893, 3297
BseMII CTCAG 6 cut(s) 152, 764, 1213, 1392, 2513, 2657
BseNI ACTGG 7 cut(s) 1913, 2287, 2440, 2461, 2938, 3025, 3226
BseRI GAGGAG 2 cut(s) 2244, 2247
BseXI GCAGC 2 cut(s) 740, 820
BsgI GTGCAG 1 cut(s) 2615
Bsh1236I CGCG 3 cut(s) 2592, 3497, 3555
BshFI GGCC 3 cut(s) 1685, 2855, 3367
BshVI ATCGAT 1 cut(s) 2976
BsiHKAI GWGCWC 5 cut(s) 160, 1200, 1585, 1630, 3181
BsiSI CCGG 3 cut(s) 391, 445, 904
BsiWI CGTACG 1 cut(s) 240
BslFI GGGAC 5 cut(s) 860, 1837, 1904, 2652, 2892
BslI CCNNNNNNNGG 4 cut(s) 1902, 2412, 2658, 3453
BsmAI GTCTC 7 cut(s) 67, 192, 208, 474, 676, 1570, 2618
BsmBI CGTCTC 4 cut(s) 192, 208, 474, 1570
BsmFI GGGAC 5 cut(s) 860, 1837, 1904, 2652, 2892
BsmI GAATGC 3 cut(s) 1025, 2195, 2570
BsnI GGCC 3 cut(s) 1685, 2855, 3367
Bsp1286I GDGCHC 5 cut(s) 160, 1200, 1585, 1630, 3181
Bsp1407I TGTACA 2 cut(s) 634, 2481
Bsp1720I GCTNAGC 3 cut(s) 1199, 1244, 2122
BspACI CCGC 8 cut(s) 731, 1429, 1591, 2303, 2592, 3364, 3458, 3553
BspANI GGCC 3 cut(s) 1685, 2855, 3367
BspCNI CTCAG 6 cut(s) 153, 763, 1212, 1393, 2512, 2656
BspDI ATCGAT 1 cut(s) 2976
BspFNI CGCG 3 cut(s) 2592, 3497, 3555
BspHI TCATGA 2 cut(s) 82, 708
BspLI GGNNCC 7 cut(s) 270, 1458, 2253, 2301, 2462, 2640, 2663
BspMAI CTGCAG 1 cut(s) 3271
BspMI ACCTGC 3 cut(s) 19, 2840, 3305
BspPI GGATC 6 cut(s) 509, 858, 914, 1014, 1752, 1756
BspQI GCTCTTC 2 cut(s) 1015, 1223
BsrBI CCGCTC 1 cut(s) 2305
BsrDI GCAATG 4 cut(s) 65, 2428, 2893, 3297
BsrFI RCCGGY 1 cut(s) 390
BsrGI TGTACA 2 cut(s) 634, 2481
BsrI ACTGG 7 cut(s) 1913, 2287, 2440, 2461, 2938, 3025, 3226
BssAI RCCGGY 1 cut(s) 390
BssECI CCNNGG 4 cut(s) 812, 2664, 2899, 3345
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 3345
Bst2UI CCWGG 4 cut(s) 1766, 2666, 2900, 3386
Bst4CI ACNGT 9 cut(s) 562, 634, 670, 688, 1546, 1731, 2200, 2335, 2951
Bst6I CTCTTC 6 cut(s) 1015, 1223, 1386, 1566, 1693, 1799
BstACI GRCGYC 1 cut(s) 201
BstAUI TGTACA 2 cut(s) 634, 2481
BstBAI YACGTR 2 cut(s) 240, 660
BstC8I GCNNGC 4 cut(s) 613, 2020, 2538, 3471
BstDSI CCRYGG 1 cut(s) 812
BstEII GGTNACC 1 cut(s) 2894
BstF5I GGATG 7 cut(s) 145, 536, 748, 2224, 2242, 3001, 3109
BstFNI CGCG 3 cut(s) 2592, 3497, 3555
BstHHI GCGC 2 cut(s) 411, 3557
BstMAI GTCTC 7 cut(s) 67, 192, 208, 474, 676, 1570, 2618
BstMWI GCNNNNNNNGC 4 cut(s) 11, 417, 944, 2001
BstNI CCWGG 4 cut(s) 1766, 2666, 2900, 3386
BstNSI RCATGY 1 cut(s) 2613
BstPI GGTNACC 1 cut(s) 2894
BstSCI CCNGG 4 cut(s) 1764, 2664, 2898, 3384
BstSFI CTRYAG 2 cut(s) 2033, 3267
BstSNI TACGTA 1 cut(s) 240
BstUI CGCG 3 cut(s) 2592, 3497, 3555
BstV1I GCAGC 2 cut(s) 740, 820
BstV2I GAAGAC 3 cut(s) 299, 2373, 2552
BstX2I RGATCY 4 cut(s) 694, 863, 1006, 1744
BstXI CCANNNNNNTGG 2 cut(s) 3392, 3481
BstYI RGATCY 4 cut(s) 694, 863, 1006, 1744
Bsu15I ATCGAT 1 cut(s) 2976
BsuRI GGCC 3 cut(s) 1685, 2855, 3367
BsuTUI ATCGAT 1 cut(s) 2976
BtgI CCRYGG 1 cut(s) 812
BtsCI GGATG 7 cut(s) 145, 536, 748, 2224, 2242, 3001, 3109
BtsI GCAGTG 2 cut(s) 1276, 2109
BtsIMutI CAGTG 4 cut(s) 1276, 2109, 2540, 3219
BveI ACCTGC 3 cut(s) 19, 2840, 3305
Cac8I GCNNGC 4 cut(s) 613, 2020, 2538, 3471
CaiI CAGNNNCTG 1 cut(s) 2041
CciI TCATGA 2 cut(s) 82, 708
CfoI GCGC 2 cut(s) 411, 3557
Cfr10I RCCGGY 1 cut(s) 390
Cfr13I GGNCC 4 cut(s) 269, 2352, 2639, 2661
ClaI ATCGAT 1 cut(s) 2976
CseI GACGC 1 cut(s) 304
Csp6I GTAC 8 cut(s) 241, 635, 1542, 1622, 1649, 1957, 2482, 3213
CviQI GTAC 8 cut(s) 241, 635, 1542, 1622, 1649, 1957, 2482, 3213
DraI TTTAAA 1 cut(s) 1483
Eam1104I CTCTTC 6 cut(s) 1015, 1223, 1386, 1566, 1693, 1799
EarI CTCTTC 6 cut(s) 1015, 1223, 1386, 1566, 1693, 1799
EciI GGCGGA 1 cut(s) 3473
Ecl136II GAGCTC 3 cut(s) 158, 1198, 1583
Eco105I TACGTA 1 cut(s) 240
Eco130I CCWWGG 1 cut(s) 3345
Eco24I GRGCYC 3 cut(s) 160, 1200, 1585
Eco32I GATATC 2 cut(s) 223, 3353
Eco47I GGWCC 4 cut(s) 269, 2352, 2639, 2661
Eco53kI GAGCTC 3 cut(s) 158, 1198, 1583
Eco57I CTGAAG 7 cut(s) 1326, 1847, 2075, 2564, 2777, 2940, 3227
Eco72I CACGTG 1 cut(s) 660
Eco91I GGTNACC 1 cut(s) 2894
EcoICRI GAGCTC 3 cut(s) 158, 1198, 1583
EcoO109I RGGNCCY 1 cut(s) 2639
EcoO65I GGTNACC 1 cut(s) 2894
EcoRII CCWGG 4 cut(s) 1764, 2664, 2898, 3384
EcoRV GATATC 2 cut(s) 223, 3353
EcoT14I CCWWGG 1 cut(s) 3345
EcoT22I ATGCAT 5 cut(s) 1491, 2242, 2428, 2570, 3297
EcoT38I GRGCYC 3 cut(s) 160, 1200, 1585
ErhI CCWWGG 1 cut(s) 3345
Esp3I CGTCTC 4 cut(s) 192, 208, 474, 1570
FaqI GGGAC 5 cut(s) 860, 1837, 1904, 2652, 2892
FauNDI CATATG 2 cut(s) 2024, 2428
FbaI TGATCA 3 cut(s) 711, 2079, 2511
Fnu4HI GCNGC 6 cut(s) 729, 732, 809, 1429, 1591, 3365
FokI GGATG 7 cut(s) 152, 543, 755, 2231, 2249, 2988, 3116
FriOI GRGCYC 3 cut(s) 160, 1200, 1585
Fsp4HI GCNGC 6 cut(s) 729, 732, 809, 1429, 1591, 3365
FspAI RTGCGCAY 1 cut(s) 410
FspI TGCGCA 1 cut(s) 410
GlaI GCGC 2 cut(s) 410, 3556
GluI GCNGC 6 cut(s) 729, 732, 809, 1429, 1591, 3365
GsuI CTGGAG 3 cut(s) 1000, 2600, 3368
HaeIII GGCC 3 cut(s) 1685, 2855, 3367
HapII CCGG 3 cut(s) 391, 445, 904
HgaI GACGC 1 cut(s) 304
HhaI GCGC 2 cut(s) 411, 3557
Hin1I GRCGYC 1 cut(s) 201
Hin6I GCGC 2 cut(s) 409, 3555
HinP1I GCGC 2 cut(s) 409, 3555
HincII GTYRAC 2 cut(s) 1143, 2812
HindII GTYRAC 2 cut(s) 1143, 2812
HindIII AAGCTT 4 cut(s) 104, 1246, 2468, 2759
HpaII CCGG 3 cut(s) 391, 445, 904
HphI GGTGA 5 cut(s) 2506, 2542, 2777, 3370, 3436
HpyCH4III ACNGT 9 cut(s) 562, 634, 670, 688, 1546, 1731, 2200, 2335, 2951
HpyCH4IV ACGT 3 cut(s) 201, 239, 659
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 417, 944, 2001
HpySE526I ACGT 3 cut(s) 201, 239, 659
Hsp92I GRCGYC 1 cut(s) 201
HspAI GCGC 2 cut(s) 409, 3555
Ksp22I TGATCA 3 cut(s) 711, 2079, 2511
LguI GCTCTTC 2 cut(s) 1015, 1223
LmnI GCTCC 7 cut(s) 155, 1081, 1195, 1588, 1675, 1973, 2257
Lsp1109I GCAGC 2 cut(s) 740, 820
LweI GCATC 7 cut(s) 130, 337, 499, 2227, 2523, 2635, 3010
MaeII ACGT 3 cut(s) 201, 239, 659
MaeIII GTNAC 7 cut(s) 770, 1040, 1929, 2894, 3376, 3491, 3515
MbiI CCGCTC 1 cut(s) 2305
MflI RGATCY 4 cut(s) 694, 863, 1006, 1744
MhlI GDGCHC 5 cut(s) 160, 1200, 1585, 1630, 3181
MluI ACGCGT 1 cut(s) 3495
MlyI GAGTC 8 cut(s) 473, 714, 963, 1263, 1886, 2047, 2542, 3435
MmeI TCCRAC 5 cut(s) 508, 1235, 1897, 2708, 3462
Mph1103I ATGCAT 5 cut(s) 1491, 2242, 2428, 2570, 3297
MroXI GAANNNNTTC 3 cut(s) 78, 128, 2370
MslI CAYNNNNRTG 3 cut(s) 435, 2385, 2886
MspA1I CMGCKG 2 cut(s) 731, 1431
MspI CCGG 3 cut(s) 391, 445, 904
MspR9I CCNGG 4 cut(s) 1766, 2666, 2900, 3386
Mva1269I GAATGC 3 cut(s) 1025, 2195, 2570
MvaI CCWGG 4 cut(s) 1766, 2666, 2900, 3386
MvnI CGCG 3 cut(s) 2592, 3497, 3555
MwoI GCNNNNNNNGC 4 cut(s) 11, 417, 944, 2001
NdeI CATATG 2 cut(s) 2024, 2428
NlaIV GGNNCC 7 cut(s) 270, 1458, 2253, 2301, 2462, 2640, 2663
NmuCI GTSAC 2 cut(s) 1040, 3376
NsbI TGCGCA 1 cut(s) 410
NsiI ATGCAT 5 cut(s) 1491, 2242, 2428, 2570, 3297
NspI RCATGY 1 cut(s) 2613
PagI TCATGA 2 cut(s) 82, 708
PaqCI CACCTGC 1 cut(s) 19
PciI ACATGT 1 cut(s) 2609
PciSI GCTCTTC 2 cut(s) 1015, 1223
PcsI WCGNNNNNNNCGW 2 cut(s) 596, 2993
PctI GAATGC 3 cut(s) 1025, 2195, 2570
PdmI GAANNNNTTC 3 cut(s) 78, 128, 2370
PfeI GAWTC 7 cut(s) 1858, 2054, 2506, 2595, 2984, 3113, 3123
Pfl23II CGTACG 1 cut(s) 240
PflMI CCANNNNNTGG 2 cut(s) 2412, 3453
PfoI TCCNGGA 1 cut(s) 1764
PkrI GCNGC 6 cut(s) 730, 733, 810, 1430, 1592, 3366
PleI GAGTC 8 cut(s) 472, 713, 962, 1263, 1886, 2046, 2542, 3435
PmaCI CACGTG 1 cut(s) 660
PmlI CACGTG 1 cut(s) 660
PpsI GAGTC 8 cut(s) 472, 713, 962, 1263, 1886, 2046, 2542, 3435
Ppu21I YACGTR 2 cut(s) 240, 660
PpuMI RGGWCCY 1 cut(s) 2639
PscI ACATGT 1 cut(s) 2609
Psp124BI GAGCTC 3 cut(s) 160, 1200, 1585
Psp5II RGGWCCY 1 cut(s) 2639
Psp6I CCWGG 4 cut(s) 1764, 2664, 2898, 3384
PspCI CACGTG 1 cut(s) 660
PspEI GGTNACC 1 cut(s) 2894
PspGI CCWGG 4 cut(s) 1764, 2664, 2898, 3384
PspLI CGTACG 1 cut(s) 240
PspN4I GGNNCC 7 cut(s) 270, 1458, 2253, 2301, 2462, 2640, 2663
PspPI GGNCC 4 cut(s) 269, 2352, 2639, 2661
PspPPI RGGWCCY 1 cut(s) 2639
PstI CTGCAG 1 cut(s) 3271
PstNI CAGNNNCTG 1 cut(s) 2041
PsuI RGATCY 4 cut(s) 694, 863, 1006, 1744
RsaI GTAC 8 cut(s) 242, 636, 1543, 1623, 1650, 1958, 2483, 3214
RsaNI GTAC 8 cut(s) 241, 635, 1542, 1622, 1649, 1957, 2482, 3213
RseI CAYNNNNRTG 3 cut(s) 435, 2385, 2886
SacI GAGCTC 3 cut(s) 160, 1200, 1585
SapI GCTCTTC 2 cut(s) 1015, 1223
SatI GCNGC 6 cut(s) 729, 732, 809, 1429, 1591, 3365
Sau96I GGNCC 4 cut(s) 269, 2352, 2639, 2661
ScaI AGTACT 1 cut(s) 3214
SchI GAGTC 8 cut(s) 473, 714, 963, 1263, 1886, 2047, 2542, 3435
ScrFI CCNGG 4 cut(s) 1766, 2666, 2900, 3386
SduI GDGCHC 5 cut(s) 160, 1200, 1585, 1630, 3181
SfaNI GCATC 7 cut(s) 130, 337, 499, 2227, 2523, 2635, 3010
SfcI CTRYAG 2 cut(s) 2033, 3267
SinI GGWCC 4 cut(s) 269, 2352, 2639, 2661
SmiMI CAYNNNNRTG 3 cut(s) 435, 2385, 2886
SmlI CTYRAG 4 cut(s) 107, 757, 2321, 3038
SmoI CTYRAG 4 cut(s) 107, 757, 2321, 3038
SnaBI TACGTA 1 cut(s) 240
SsiI CCGC 8 cut(s) 731, 1429, 1591, 2303, 2592, 3364, 3458, 3553
SstI GAGCTC 3 cut(s) 160, 1200, 1585
StyD4I CCNGG 4 cut(s) 1764, 2664, 2898, 3384
StyI CCWWGG 1 cut(s) 3345
TaaI ACNGT 9 cut(s) 562, 634, 670, 688, 1546, 1731, 2200, 2335, 2951
TaiI ACGT 3 cut(s) 204, 242, 662
TaqI TCGA 8 cut(s) 216, 957, 1598, 2061, 2169, 2782, 2798, 2976
TatI WGTACW 4 cut(s) 634, 1541, 2481, 3212
TauI GCSGC 4 cut(s) 734, 1431, 1593, 3367
TfiI GAWTC 7 cut(s) 1858, 2054, 2506, 2595, 2984, 3113, 3123
TscAI CASTG 4 cut(s) 1283, 2116, 2547, 3226
TseFI GTSAC 2 cut(s) 1040, 3376
TseI GCWGC 2 cut(s) 728, 808
Tsp45I GTSAC 2 cut(s) 1040, 3376
TspGWI ACGGA 2 cut(s) 261, 829
TspRI CASTG 4 cut(s) 1283, 2116, 2547, 3226
Van91I CCANNNNNTGG 2 cut(s) 2412, 3453
VpaK11BI GGWCC 4 cut(s) 269, 2352, 2639, 2661
XapI RAATTY 5 cut(s) 1478, 2046, 2735, 2768, 2801
XceI RCATGY 1 cut(s) 2613
XcmI CCANNNNNNNNNTGG 3 cut(s) 315, 1967, 2471
XmnI GAANNNNTTC 3 cut(s) 78, 128, 2370
ZraI GACGTC 1 cut(s) 202
ZrmI AGTACT 1 cut(s) 3214
Zsp2I ATGCAT 5 cut(s) 1491, 2242, 2428, 2570, 3297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.