RchiOBHm_Chr6g0278191

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000110
Physical Location & Seq
Forward (+)
41181293 .. 41184412
3120 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24956

Sequence Viewer

Length: 2754 bp
ATGGCCATGGCTTCAGCTGCAATAGACCTTTTGCTTGGGAAAATAGTGTCATTTCTTGACGACCAATTGTCACTGTTAGGAGGAGTTCATGATGAACTTGAAGAAATCAAGCGCGAGCTTATAGTCATGAAAGCCTTCTTGGAGGATGCAGAAAGAACCGGTGTTCTCAGTGAAGTGGAGAAAACATGGGTGGCGAATGTGAGGGACGTATCCGTAAACGTTGAAGACTTCATTGACGAGTTCACCTACCACATCAACAACCAGAGAAGTTGGAGTCCATTTACAAGAACGCTTCGTAAATCCCTTCGCTTCCCTAAGAATCTTTGGGAGAGGCATCGGATTGCCACAAAGTTGCAGAAGATCATCAAAATAATCAAAGCCATTCCAGAGAGAAGCCTACGTTACGGAGTTAAACGTATAGAAGGTGTTTCTTATTCCCACTACGATCCTGATCGGCTGAGAATCTTAAGGGAGTCATCTCTTTTCCTTCAAGATGATGAAGTGGTGGGAATTATAGATGCTAAAGAGAAGTTGGTCACAATGTTGTTGTCTTCACAAGCTCAAAGAACTGTGATTTCTGTTGTTGGAATGGGTGGCTCTGGCAAGACTACCTTGGTTGCAAAGACCTTCAATGGCCAGACTGTAAAGCAACATTTCGATTGCTGTGCGTGGATTACTGTCTCGCAAACGTACACGGTTGAAGATCTACTCAGAGTAATCATGAAGGAGCTTCTCCGAGCAGTGAAGGAGCAGATTCCAGTAGACTTGAGCGACATGAGCTATTCACATTTGGTGGAAATGCTGGCCAGCTATTTGAAGGAAAAGAGGTACATGATTGTCTTAGATGATGTGTGGGATATCAATCTTTGGAGAGAAACAAGTGGGGCACTTCCAGATGGACCACATGGAAGCCGGGTCATGCTTACAACTCGTAAAGAAGACATTGCTTCTTTTCCATTTGGGCTTGGAAGTTATGTCCACCATGTTCAGCCTCTAAACAAGAGTGAGGCATGGCATCTTTTTTCCATGAAAGCTTTCTTCAGTTGTCCTAATAGCTGCTGTCCAACTGAGCTCGAACATATAGCTAGAGATCTTGTTGACAAATGCGAAGGCTTACCTCTAGGAATTGTTGCTTTGGGCGCTCTCATGTCAACCAAAAGTGTTCTGTCCGAGTGGATGAAGGTTTACGGCAGCTTGAACTGGGAGCTATGCAACAACCCTAGGCTTGAAGTGGTGAAAAGCATCTTACTGTTGAGCTTCAATGATTTACCATATCGGCTAAAGCATTGCTTCCTATATTGTTGTGTTTTCCCCGAAGATTATGTGATCCAGTGTAGAAGGCTTATTAGACTGTGGATAGCGGAGGGGTTTGTGGAACAAGTTAGAGGTGCTAAGACAGAAGAGATAGCTGAGAGCTACATGGCGGAGCTCACGTGTCGATGCATGCTTCAAGTAGTTGAGAGGGAACCATCTGGGAGGGCAGAAACATTTAGGATGCATGATCTTCTGCGAGAACTCTGCATTTCAATTTCAGAAGCTGAGAACTTTTGTACCATATACAATGACCAAGAACCAGCTAAAGAGAGCAAGGCTCGCCGCGTGTCAATGCAAGCGAATTCTCGAGCACTAAGAAATCCGAAAGTCATTTCGAAGGTTCGCACATTTTTCATGTTTGCTGCTTTTTCATTGAATTATCTGCCATCTGGTTTCCAATTGTTGAGAATCCTAGACCTTGAAGATGTTCCGATTGTGCAGTTGCCAGATGACATAGTGAACTGCTTCAATTTGATGTATTTAAATTTGAGAAGCACAAAAGTTAAAGAGCTTCCCAAAGACATAGGGAAACTCAGCAACTTAGAAATGCTAAACATAAGCAATTCGAAAATTAGATTACTTCCGGTTGGGATTTTGAAGTTACAGAAGCTACACCATCTCTTGATGTTGCATTTTAATGATCAAAAGTTGTGTTCTTTTGATTGTCTTGACGGCTGCACACAAGCTCCGCCGGGCATTTGTAAGCTAAAGAGCTTACAAGTTCTGGATACAGTCAAGGTGGAAGGCGAGTTGATCGAACAACTTCAGTCCATGACGCAACTCACAAGACTAGGCTTAGCAAATGTGAGGGAAGCTGATGAAAAAGATCTATGCAAGGCGCTCGAAAACATGAAACTCATTGAGCAGTTGAATGTCATGACAACCGATGAGGATGAGGTGTTGCGCATGGACGCATTAGCGGAGGGTCCTCCAATGCTCAACACACTTTTCCTAACTGGAAAGTTAGAGAGGATTCCACTTTGGTTCCATTCTCTTCATAGCCTTTCGTACTTGGCTTTGCTCTGGTCTAGAGTAACACATGATTTTCTTCCTTGCATCCAAGAGCTGCCAAATTTGACAAGGCTGTGGTTGATTAATGCATATACAGGAGAAGAGTTAGTCTTTCGCAGTGGATTCCGAAAGCTCACCGAGTTGATACTGCTCAACTTTTCTCAGTTGTCTGTGATCATCATAGAGAAGGGAGTGATGCCGGCATTAAAGACACTGGATATATGGGAATGCATGCAGTTGAAGCAAATTCCTCGGGGCATTGAACATCTCACAAATCTCAAACAATTGACTCTGTTAGGTGTTCCCAATGAACTGATTGAGTGCATGCGAGGAGAAGGAAGCATGGATAACTCAAAGGTTAAGCAGATTCCCAACATTGGTTATTGCTACAAAACTAGGTTTGGGTGGTCTCATGGTAGAACGCGATGA

Protein Analysis

917

Amino Acids

105.01

Weight (kDa)

6.74

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 92 4.3e-22 Rx N-terminal domain
NB-ARC PF00931 175 - 346 6.5e-45 NB-ARC domain
WHD_DRP PF23559 436 - 506 6.7e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 551 - 872 2.7e-42 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2219
AccI GTMKAC 1 cut(s) 762
AccII CGCG 3 cut(s) 114, 1599, 2749
AciI CCGC 5 cut(s) 1361, 1424, 1597, 2003, 2234
AclI AACGTT 1 cut(s) 219
AclWI GGATC 2 cut(s) 440, 1321
AcoI YGGCCR 3 cut(s) 3, 634, 804
AcsI RAATTY 4 cut(s) 1615, 1798, 2386, 2571
AcuI CTGAAG 2 cut(s) 1024, 2063
AcvI CACGTG 1 cut(s) 1434
AfaI GTAC 4 cut(s) 692, 830, 1552, 2324
AfiI CCNNNNNNNGG 1 cut(s) 2702
AflII CTTAAG 1 cut(s) 466
AflIII ACRYGT 1 cut(s) 1433
AgeI ACCGGT 1 cut(s) 158
AhdI GACNNNNNGTC 1 cut(s) 67
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
Alw21I GWGCWC 3 cut(s) 1074, 1431, 1627
Alw26I GTCTC 2 cut(s) 685, 2739
AlwI GGATC 2 cut(s) 440, 1321
AlwNI CAGNNNCTG 1 cut(s) 1538
Ama87I CYCGRG 2 cut(s) 1620, 2577
AoxI GGCC 3 cut(s) 3, 634, 804
ApeKI GCWGC 6 cut(s) 17, 1056, 1191, 1676, 1989, 2380
ApoI RAATTY 4 cut(s) 1615, 1798, 2386, 2571
AseI ATTAAT 1 cut(s) 2409
AsiGI ACCGGT 1 cut(s) 158
Asp700I GAANNNNTTC 5 cut(s) 134, 1034, 1740, 1778, 2076
AspA2I CCTAGG 1 cut(s) 1220
AspLEI GCGC 4 cut(s) 114, 1142, 2155, 2220
AspS9I GGNCC 2 cut(s) 899, 2240
AsuC2I CCSGG 2 cut(s) 914, 2007
AsuHPI GGTGA 3 cut(s) 235, 1246, 2452
AsuII TTCGAA 2 cut(s) 1649, 1880
AvaI CYCGRG 2 cut(s) 1620, 2577
AvaII GGWCC 2 cut(s) 899, 2240
AvrII CCTAGG 1 cut(s) 1220
BaeGI GKGCMC 1 cut(s) 889
BaeI ACNNNNGTAYC 2 cut(s) 1534, 1567
BalI TGGCCA 3 cut(s) 5, 636, 806
BanII GRGCYC 2 cut(s) 1074, 1431
BbrPI CACGTG 1 cut(s) 1434
BbsI GAAGAC 3 cut(s) 231, 543, 945
Bbv12I GWGCWC 3 cut(s) 1074, 1431, 1627
BbvI GCAGC 6 cut(s) 4, 1043, 1203, 1663, 1976, 2367
BccI CCATC 4 cut(s) 890, 1477, 1708, 1938
BceAI ACGGC 2 cut(s) 1204, 2002
BcgI CGANNNNNNTGC 8 cut(s) 647, 681, 1500, 1534, 2137, 2171, 2558, 2592
BciVI GTATCC 2 cut(s) 220, 2035
BclI TGATCA 2 cut(s) 1954, 2499
BcnI CCSGG 2 cut(s) 914, 2007
BcoDI GTCTC 2 cut(s) 685, 2739
BfaI CTAG 7 cut(s) 1086, 1121, 1221, 1727, 2105, 2343, 2721
BfoI RGCGCY 2 cut(s) 1143, 2156
BfrI CTTAAG 1 cut(s) 466
BfuI GTATCC 2 cut(s) 220, 2035
BglII AGATCT 3 cut(s) 703, 1090, 2140
BisI GCNGC 7 cut(s) 18, 1057, 1192, 1597, 1677, 1990, 2381
BlnI CCTAGG 1 cut(s) 1220
BlpI GCTNAGC 1 cut(s) 2110
BlsI GCNGC 7 cut(s) 19, 1058, 1193, 1598, 1678, 1991, 2382
Bme1390I CCNGG 2 cut(s) 914, 2007
Bme18I GGWCC 2 cut(s) 899, 2240
BmeRI GACNNNNNGTC 1 cut(s) 67
BmeT110I CYCGRG 2 cut(s) 1620, 2577
BmgT120I GGNCC 2 cut(s) 899, 2240
BmiI GGNNCC 3 cut(s) 1467, 2241, 2300
BmrFI CCNGG 2 cut(s) 914, 2007
BmrI ACTGGG 1 cut(s) 1210
BmsI GCATC 9 cut(s) 136, 343, 508, 1024, 1251, 1430, 1485, 2379, 2511
BmuI ACTGGG 1 cut(s) 1210
BpiI GAAGAC 3 cut(s) 231, 543, 945
BplI GAGNNNNNCTC 2 cut(s) 1576, 1608
Bpu1102I GCTNAGC 1 cut(s) 2110
Bpu14I TTCGAA 2 cut(s) 1649, 1880
BpuEI CTTGAG 1 cut(s) 787
BpuMI CCSGG 2 cut(s) 914, 2007
BsaAI YACGTR 1 cut(s) 1434
BsaBI GATNNNNATC 2 cut(s) 450, 861
BsaI GGTCTC 1 cut(s) 2739
BsaJI CCNNGG 4 cut(s) 6, 612, 1220, 2576
BsaWI WCCGGW 2 cut(s) 158, 1897
BsaXI ACNNNNNCTCC 4 cut(s) 1418, 1448, 1984, 2014
Bsc4I CCNNNNNNNGG 1 cut(s) 2702
Bse118I RCCGGY 2 cut(s) 158, 2524
Bse1I ACTGG 5 cut(s) 758, 1205, 1330, 2275, 2544
Bse3DI GCAATG 2 cut(s) 942, 1285
Bse8I GATNNNNATC 2 cut(s) 450, 861
BseDI CCNNGG 4 cut(s) 6, 612, 1220, 2576
BseGI GGATG 5 cut(s) 151, 1182, 1500, 2212, 2370
BseJI GATNNNNATC 2 cut(s) 450, 861
BseLI CCNNNNNNNGG 1 cut(s) 2702
BseMI GCAATG 2 cut(s) 942, 1285
BseMII CTCAG 8 cut(s) 181, 449, 724, 1059, 1401, 1530, 1861, 2501
BseNI ACTGG 5 cut(s) 758, 1205, 1330, 2275, 2544
BseRI GAGGAG 2 cut(s) 96, 2670
BseSI GKGCMC 1 cut(s) 889
BseXI GCAGC 6 cut(s) 4, 1043, 1203, 1663, 1976, 2367
BsgI GTGCAG 2 cut(s) 1772, 1975
Bsh1236I CGCG 3 cut(s) 114, 1599, 2749
BshFI GGCC 3 cut(s) 5, 636, 806
BshTI ACCGGT 1 cut(s) 158
BsiHKAI GWGCWC 3 cut(s) 1074, 1431, 1627
BsiHKCI CYCGRG 2 cut(s) 1620, 2577
BsiSI CCGG 5 cut(s) 159, 913, 1898, 2006, 2525
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 1 cut(s) 2702
BsmAI GTCTC 2 cut(s) 685, 2739
BsmFI GGGAC 1 cut(s) 218
BsmI GAATGC 1 cut(s) 2558
BsnI GGCC 3 cut(s) 5, 636, 806
Bso31I GGTCTC 1 cut(s) 2739
BsoBI CYCGRG 2 cut(s) 1620, 2577
Bsp119I TTCGAA 2 cut(s) 1649, 1880
Bsp1286I GDGCHC 4 cut(s) 889, 1074, 1431, 1627
Bsp1720I GCTNAGC 1 cut(s) 2110
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 5 cut(s) 1361, 1424, 1597, 2003, 2234
BspANI GGCC 3 cut(s) 5, 636, 806
BspCNI CTCAG 8 cut(s) 180, 450, 723, 1060, 1402, 1531, 1860, 2500
BspFNI CGCG 3 cut(s) 114, 1599, 2749
BspHI TCATGA 4 cut(s) 88, 126, 720, 2190
BspLI GGNNCC 3 cut(s) 1467, 2241, 2300
BspPI GGATC 2 cut(s) 440, 1321
BspT104I TTCGAA 2 cut(s) 1649, 1880
BspTI CTTAAG 1 cut(s) 466
BspTNI GGTCTC 1 cut(s) 2739
BsrDI GCAATG 2 cut(s) 942, 1285
BsrFI RCCGGY 2 cut(s) 158, 2524
BsrI ACTGG 5 cut(s) 758, 1205, 1330, 2275, 2544
BssAI RCCGGY 2 cut(s) 158, 2524
BssECI CCNNGG 4 cut(s) 6, 612, 1220, 2576
BssT1I CCWWGG 3 cut(s) 6, 612, 1220
Bst4CI ACNGT 8 cut(s) 75, 571, 643, 679, 697, 1251, 1353, 2047
Bst6I CTCTTC 3 cut(s) 1395, 2313, 2421
BstAFI CTTAAG 1 cut(s) 466
BstBAI YACGTR 1 cut(s) 1434
BstBI TTCGAA 2 cut(s) 1649, 1880
BstC8I GCNNGC 9 cut(s) 116, 804, 808, 1445, 1594, 1611, 2526, 2558, 2651
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 5 cut(s) 151, 1182, 1500, 2212, 2370
BstFNI CGCG 3 cut(s) 114, 1599, 2749
BstH2I RGCGCY 2 cut(s) 1143, 2156
BstHHI GCGC 4 cut(s) 114, 1142, 2155, 2220
BstMAI GTCTC 2 cut(s) 685, 2739
BstMWI GCNNNNNNNGC 5 cut(s) 17, 777, 1139, 1593, 2566
BstNSI RCATGY 3 cut(s) 1447, 2560, 2653
BstSCI CCNGG 2 cut(s) 912, 2005
BstSLI GKGCMC 1 cut(s) 889
BstUI CGCG 3 cut(s) 114, 1599, 2749
BstV1I GCAGC 6 cut(s) 4, 1043, 1203, 1663, 1976, 2367
BstV2I GAAGAC 3 cut(s) 231, 543, 945
BstX2I RGATCY 3 cut(s) 703, 1090, 2140
BstYI RGATCY 3 cut(s) 703, 1090, 2140
BsuI GTATCC 2 cut(s) 220, 2035
BsuRI GGCC 3 cut(s) 5, 636, 806
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 5 cut(s) 151, 1182, 1500, 2212, 2370
BtsI GCAGTG 2 cut(s) 747, 2449
BtsIMutI CAGTG 6 cut(s) 71, 175, 747, 1337, 2449, 2537
Cac8I GCNNGC 9 cut(s) 116, 804, 808, 1445, 1594, 1611, 2526, 2558, 2651
CaiI CAGNNNCTG 1 cut(s) 1538
CciI TCATGA 4 cut(s) 88, 126, 720, 2190
CfoI GCGC 4 cut(s) 114, 1142, 2155, 2220
Cfr10I RCCGGY 2 cut(s) 158, 2524
Cfr13I GGNCC 2 cut(s) 899, 2240
CseI GACGC 2 cut(s) 2098, 2234
Csp6I GTAC 4 cut(s) 691, 829, 1551, 2323
CspAI ACCGGT 1 cut(s) 158
CviQI GTAC 4 cut(s) 691, 829, 1551, 2323
DraI TTTAAA 1 cut(s) 1797
DriI GACNNNNNGTC 1 cut(s) 67
EaeI YGGCCR 3 cut(s) 3, 634, 804
Eam1104I CTCTTC 3 cut(s) 1395, 2313, 2421
Eam1105I GACNNNNNGTC 1 cut(s) 67
EarI CTCTTC 3 cut(s) 1395, 2313, 2421
EciI GGCGGA 2 cut(s) 1439, 1992
Ecl136II GAGCTC 2 cut(s) 1072, 1429
Eco130I CCWWGG 3 cut(s) 6, 612, 1220
Eco24I GRGCYC 2 cut(s) 1074, 1431
Eco31I GGTCTC 1 cut(s) 2739
Eco32I GATATC 1 cut(s) 859
Eco47I GGWCC 2 cut(s) 899, 2240
Eco53kI GAGCTC 2 cut(s) 1072, 1429
Eco57I CTGAAG 2 cut(s) 1024, 2063
Eco72I CACGTG 1 cut(s) 1434
Eco88I CYCGRG 2 cut(s) 1620, 2577
EcoICRI GAGCTC 2 cut(s) 1072, 1429
EcoO109I RGGNCCY 1 cut(s) 2240
EcoRI GAATTC 1 cut(s) 1615
EcoRV GATATC 1 cut(s) 859
EcoT14I CCWWGG 3 cut(s) 6, 612, 1220
EcoT22I ATGCAT 4 cut(s) 1445, 1500, 2416, 2558
EcoT38I GRGCYC 2 cut(s) 1074, 1431
ErhI CCWWGG 3 cut(s) 6, 612, 1220
FalI AAGNNNNNCTT 4 cut(s) 596, 628, 1274, 1306
FaqI GGGAC 1 cut(s) 218
FbaI TGATCA 2 cut(s) 1954, 2499
FblI GTMKAC 1 cut(s) 762
Fnu4HI GCNGC 7 cut(s) 18, 1057, 1192, 1597, 1677, 1990, 2381
FokI GGATG 5 cut(s) 158, 1189, 1507, 2219, 2357
FriOI GRGCYC 2 cut(s) 1074, 1431
Fsp4HI GCNGC 7 cut(s) 18, 1057, 1192, 1597, 1677, 1990, 2381
FspBI CTAG 7 cut(s) 1086, 1121, 1221, 1727, 2105, 2343, 2721
FspI TGCGCA 1 cut(s) 2219
GlaI GCGC 4 cut(s) 113, 1141, 2154, 2219
GluI GCNGC 7 cut(s) 18, 1057, 1192, 1597, 1677, 1990, 2381
HaeII RGCGCY 2 cut(s) 1143, 2156
HaeIII GGCC 3 cut(s) 5, 636, 806
HapII CCGG 5 cut(s) 159, 913, 1898, 2006, 2525
HgaI GACGC 2 cut(s) 2098, 2234
HhaI GCGC 4 cut(s) 114, 1142, 2155, 2220
Hin6I GCGC 4 cut(s) 112, 1140, 2153, 2218
HinP1I GCGC 4 cut(s) 112, 1140, 2153, 2218
HincII GTYRAC 2 cut(s) 1099, 1152
HindII GTYRAC 2 cut(s) 1099, 1152
HindIII AAGCTT 1 cut(s) 1032
HpaII CCGG 5 cut(s) 159, 913, 1898, 2006, 2525
HphI GGTGA 3 cut(s) 235, 1246, 2452
Hpy166II GTNNAC 9 cut(s) 217, 243, 693, 763, 979, 1099, 1152, 1186, 1774
Hpy188I TCNGA 8 cut(s) 339, 713, 737, 1171, 1534, 1638, 1746, 2453
Hpy8I GTNNAC 9 cut(s) 217, 243, 693, 763, 979, 1099, 1152, 1186, 1774
HpyCH4III ACNGT 8 cut(s) 75, 571, 643, 679, 697, 1251, 1353, 2047
HpyCH4IV ACGT 6 cut(s) 207, 219, 400, 415, 689, 1433
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 777, 1139, 1593, 2566
HpySE526I ACGT 6 cut(s) 207, 219, 400, 415, 689, 1433
HspAI GCGC 4 cut(s) 112, 1140, 2153, 2218
KroI GCCGGC 1 cut(s) 2524
KroNI GCCGGC 1 cut(s) 2526
Ksp22I TGATCA 2 cut(s) 1954, 2499
LmnI GCTCC 5 cut(s) 727, 748, 1204, 1426, 2005
Lsp1109I GCAGC 6 cut(s) 4, 1043, 1203, 1663, 1976, 2367
LweI GCATC 9 cut(s) 136, 343, 508, 1024, 1251, 1430, 1485, 2379, 2511
MaeI CTAG 7 cut(s) 1086, 1121, 1221, 1727, 2105, 2343, 2721
MaeII ACGT 6 cut(s) 207, 219, 400, 415, 689, 1433
MaeIII GTNAC 5 cut(s) 69, 401, 535, 1914, 2347
MfeI CAATTG 3 cut(s) 65, 1712, 2609
MflI RGATCY 3 cut(s) 703, 1090, 2140
MhlI GDGCHC 4 cut(s) 889, 1074, 1431, 1627
MlsI TGGCCA 3 cut(s) 5, 636, 806
MluNI TGGCCA 3 cut(s) 5, 636, 806
MlyI GAGTC 3 cut(s) 283, 482, 2608
MmeI TCCRAC 3 cut(s) 251, 565, 1088
Mox20I TGGCCA 3 cut(s) 5, 636, 806
Mph1103I ATGCAT 4 cut(s) 1445, 1500, 2416, 2558
MroNI GCCGGC 1 cut(s) 2524
MroXI GAANNNNTTC 5 cut(s) 134, 1034, 1740, 1778, 2076
MscI TGGCCA 3 cut(s) 5, 636, 806
MseI TTAA 8 cut(s) 411, 467, 1796, 1818, 1950, 2409, 2531, 2685
MslI CAYNNNNRTG 1 cut(s) 1950
Msp20I TGGCCA 3 cut(s) 5, 636, 806
MspA1I CMGCKG 1 cut(s) 17
MspCI CTTAAG 1 cut(s) 466
MspI CCGG 5 cut(s) 159, 913, 1898, 2006, 2525
MspR9I CCNGG 2 cut(s) 914, 2007
MunI CAATTG 3 cut(s) 65, 1712, 2609
Mva1269I GAATGC 1 cut(s) 2558
MvnI CGCG 3 cut(s) 114, 1599, 2749
MwoI GCNNNNNNNGC 5 cut(s) 17, 777, 1139, 1593, 2566
NaeI GCCGGC 1 cut(s) 2526
NciI CCSGG 2 cut(s) 914, 2007
NcoI CCATGG 1 cut(s) 6
NgoMIV GCCGGC 1 cut(s) 2524
NlaIV GGNNCC 3 cut(s) 1467, 2241, 2300
NmuCI GTSAC 2 cut(s) 69, 535
NsbI TGCGCA 1 cut(s) 2219
NsiI ATGCAT 4 cut(s) 1445, 1500, 2416, 2558
NspI RCATGY 3 cut(s) 1447, 2560, 2653
NspV TTCGAA 2 cut(s) 1649, 1880
PaeI GCATGC 3 cut(s) 1447, 2560, 2653
PaeR7I CTCGAG 1 cut(s) 1620
PagI TCATGA 4 cut(s) 88, 126, 720, 2190
PctI GAATGC 1 cut(s) 2558
PdiI GCCGGC 1 cut(s) 2526
PdmI GAANNNNTTC 5 cut(s) 134, 1034, 1740, 1778, 2076
PfeI GAWTC 7 cut(s) 319, 462, 754, 1722, 2287, 2448, 2692
PinAI ACCGGT 1 cut(s) 158
PkrI GCNGC 7 cut(s) 19, 1058, 1193, 1598, 1678, 1991, 2382
PleI GAGTC 3 cut(s) 282, 481, 2608
PmaCI CACGTG 1 cut(s) 1434
PmlI CACGTG 1 cut(s) 1434
PpsI GAGTC 3 cut(s) 282, 481, 2608
Ppu21I YACGTR 1 cut(s) 1434
PpuMI RGGWCCY 1 cut(s) 2240
PshBI ATTAAT 1 cut(s) 2409
Psp124BI GAGCTC 2 cut(s) 1074, 1431
Psp1406I AACGTT 1 cut(s) 219
Psp5II RGGWCCY 1 cut(s) 2240
PspCI CACGTG 1 cut(s) 1434
PspN4I GGNNCC 3 cut(s) 1467, 2241, 2300
PspPI GGNCC 2 cut(s) 899, 2240
PspPPI RGGWCCY 1 cut(s) 2240
PstNI CAGNNNCTG 1 cut(s) 1538
PsuI RGATCY 3 cut(s) 703, 1090, 2140
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 4 cut(s) 692, 830, 1552, 2324
RsaNI GTAC 4 cut(s) 691, 829, 1551, 2323
RseI CAYNNNNRTG 1 cut(s) 1950
SacI GAGCTC 2 cut(s) 1074, 1431
SaqAI TTAA 8 cut(s) 411, 467, 1796, 1818, 1950, 2409, 2531, 2685
SatI GCNGC 7 cut(s) 18, 1057, 1192, 1597, 1677, 1990, 2381
Sau96I GGNCC 2 cut(s) 899, 2240
SchI GAGTC 3 cut(s) 283, 482, 2608
ScrFI CCNGG 2 cut(s) 914, 2007
SduI GDGCHC 4 cut(s) 889, 1074, 1431, 1627
SfaNI GCATC 9 cut(s) 136, 343, 508, 1024, 1251, 1430, 1485, 2379, 2511
Sfr274I CTCGAG 1 cut(s) 1620
SfuI TTCGAA 2 cut(s) 1649, 1880
SinI GGWCC 2 cut(s) 899, 2240
SlaI CTCGAG 1 cut(s) 1620
SmiI ATTTAAAT 1 cut(s) 1797
SmiMI CAYNNNNRTG 1 cut(s) 1950
SmlI CTYRAG 3 cut(s) 466, 766, 1620
SmoI CTYRAG 3 cut(s) 466, 766, 1620
SphI GCATGC 3 cut(s) 1447, 2560, 2653
SsiI CCGC 5 cut(s) 1361, 1424, 1597, 2003, 2234
SspMI CTAG 7 cut(s) 1086, 1121, 1221, 1727, 2105, 2343, 2721
SstI GAGCTC 2 cut(s) 1074, 1431
StyD4I CCNGG 2 cut(s) 912, 2005
StyI CCWWGG 3 cut(s) 6, 612, 1220
SwaI ATTTAAAT 1 cut(s) 1797
TaaI ACNGT 8 cut(s) 75, 571, 643, 679, 697, 1251, 1353, 2047
TaiI ACGT 6 cut(s) 210, 222, 403, 418, 692, 1436
TaqI TCGA 8 cut(s) 657, 1074, 1438, 1621, 1649, 1880, 2070, 2157
TauI GCSGC 1 cut(s) 1599
TfiI GAWTC 7 cut(s) 319, 462, 754, 1722, 2287, 2448, 2692
Tru1I TTAA 8 cut(s) 411, 467, 1796, 1818, 1950, 2409, 2531, 2685
Tru9I TTAA 8 cut(s) 411, 467, 1796, 1818, 1950, 2409, 2531, 2685
TscAI CASTG 6 cut(s) 78, 175, 747, 1337, 2449, 2544
TseFI GTSAC 2 cut(s) 69, 535
TseI GCWGC 6 cut(s) 17, 1056, 1191, 1676, 1989, 2380
Tsp45I GTSAC 2 cut(s) 69, 535
TspGWI ACGGA 2 cut(s) 202, 420
TspRI CASTG 6 cut(s) 78, 175, 747, 1337, 2449, 2544
Vha464I CTTAAG 1 cut(s) 466
VpaK11BI GGWCC 2 cut(s) 899, 2240
VspI ATTAAT 1 cut(s) 2409
XapI RAATTY 4 cut(s) 1615, 1798, 2386, 2571
XbaI TCTAGA 1 cut(s) 2342
XceI RCATGY 3 cut(s) 1447, 2560, 2653
XhoI CTCGAG 1 cut(s) 1620
XmaJI CCTAGG 1 cut(s) 1220
XmiI GTMKAC 1 cut(s) 762
XmnI GAANNNNTTC 5 cut(s) 134, 1034, 1740, 1778, 2076
XspI CTAG 7 cut(s) 1086, 1121, 1221, 1727, 2105, 2343, 2721
Zsp2I ATGCAT 4 cut(s) 1445, 1500, 2416, 2558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.