Rw5G011970

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000110
Physical Location & Seq
Forward (+)
13933113 .. 13936100
2988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G011970.1

Sequence Viewer

Length: 2763 bp
ATGGCCTTATCTGCAGCAGTCCTATTAATTGGAAAACTTGGGGCAATTCTTGAAAACGAAAGAACAACCATAGCCAGTGTTCGTGAAGAAGTTGATAGCATTAGGAAGGAGCTTGAAAGCATGAAAGCTTTCCTAGTGGATGCGGAGGCCTACACAGCAAAAACTGAAACAGAGAAATTGTGGATTGCAAGCATCAGAGACTTGGCCTATGAAGTTGAAGATATCATTGATGAATTCATGTATCACATGCATGAGAAGCAAAGTGGGAGTCGACTTTCAAGGAGGCTACACAAAACCATTTGCTTTCCAAAAAGTCTTTGGTTTAGACGTAAAACTGCAAAGAGGTTACAGAAAATCACTAAAAAGATCAAAGACGAATCAGAAAGGAGTCAAAGGTTCAGTGTTGGCCCTGTAGGAGGAGCAACTACATCTGAAGATATTCACAGATTGGTGCAGAACCAAGCGGAGTCTTCGTTTTTCAGTATGGAAGACGAACTGGTTGGGATTGAAGGCAAGAAGCAAATACTAATGGGATGGCTCATGGATGAAGATCAACACCAAACTGTTATATCTGTGGTTGGCATGGGAGGTTCTGGAAAGACAACTCTAGCTGCCAAGACCTTCAACAATGAAGGGGTGAAGAGACATTTTCATTGTTGTGCGTGGATTACAGTTTCCCAAACTTTTCATGATGAGGGACACTTGTTTCGAAGCTTGATCATGGAATTCTACAAATCAAGAAAGGAAGATGTCCCTGAAAATTTGAATACCATGAGCAGGAGAGAATTGATAGAGTTACTAGTTAACTACTTGGAGTCCAAAAAATACCTGGTTGTTCTAGATGACGTGTGGGATATAAAAGTTTGGAGAGAAATAAAGGTATCACTTCAAGATAGGCAACTCGGAAGTCGTATCATAGTTACAACTCGAAAAGAAGAAGTAGCAGCCAATTCTTTTGGAGTTAAAAGCCATGTTCACCATATTCAACCCCTGCTAAAGAATGAGGCTTGGGAGCTCTTCTGCAAAAAAGCATTCTCATCTAACGAGCACAAAACTTGTCCACCTGATTTTGTGTCATTAGCTTGCCAACTTGTGGAAAAGTGTGAAGGCCTTCCTCTGGCTATTGTGGGTTTAGGTGGTCTTATGTCTTCCAAGAAGTCGCTAGATCAATGGCAGCAAGTCTTCAACAGCTTGAATTGGCATATGGATAACAATTCATTGCTAGACCCTGTGAAAAACATCTTGTTGCTGAGTTTCAATGATCTGCCATCTCAATTGAAACATTGCTTCCTCTATTGCTCTCTATTCCCAGAAGATTTTGTAATTAGAAGAAAAAGGCTCATTAGATTGTGGATAGCTGAAGGATTTGTCGAACATGTGAAAGGGATCACGCCCGAAGAAGTTGCAGATGGCTATCTTTGGGAACTCTGCTTCCGTAGCATGTTACAAGTTGTAAAGAGAAATGGAACAGGAAGACCGAAACAAGTTAAGATGCATGATCTGATGCGAGAGCTTGCTTTGTCGATAGCCGAGAGGGAAAATTTTGGCCATGTATACGACGGGAGAGAAGGAATGGAAGAGAACTCAACCCGTCGCTTGTCAATTCACACAATGAGTAATGGAGGAGAAATTCAACCATGGCCGGGTATGTCAAAGATTCATTCTCTTCTTATCTTTGCAACTGACATGTCCTCATTGTCTTTCTTAAATACACTGGTTTCTCGATGTAATTTGTTGAGGACTCTAGACTTGGAGGATGTCCAAATTGATAAACTGCCAGATGCAGTTGTTTACTTGTTCAACTTGAGATATTTAAATTTGAGTGGTACTTTAATCAAGGAACTTCCAAAGTCCATAGGGCGTCTCCGCAACCTTCAATATTTGAAAATCGAGGAAACCATGATAGAGGCAATTCCACAAGGAATTGCAAAGTTGTTGAACCTGCATTATCTAACTATGTTTCGGTTGACTGGAACCACTCTGGACTTCAAAGTTGCGAGTGGGACAAAAGCGCCATCAAATATTTGTCAGTTACAGAAATTGCAATGTTTATCAATGATTGAATCAGAAGGGGATACTATTAGACTTATCGGCAACATGACTCAACTCAGGGTCATCGGCATATCGAATGTGAAAGAAAGAGATGAGATTGACCTTTGGGTCTCAATTGAAAAGTTGACTCTGCTTCAATCTTTGCTTTTAATGGCAAGTAATGAAGAGGAAATCCTTCCGGTGAAAGCACAATTTCCCTCACTTCCGCACCTTCAAAAGCTTCAATTGATTGGTAGACTGCAAAACCTAATGCCTTGGTTTTCTTCACTGCATAGCCTTACAACTCTAGTTCTGCACTGGTCAAGACTAGAAGATGATGTACTACCTCAAATTGAAGCACTGCCCAATTTGACAGAGCTTATTCTTTGTAATGCATATGTTGGGGAAGAGTTGTGTTTCCGCAGAGGTTTTGTAAAGCTGGAGAGGTTGCATTTGTTGAATTTTGCCTCGGTGAAAAAGATTACTATAGAAAAAGGAGTGATGCCAAATCTCCGGGACTTAATGGTTGAACAGTTCATGGAATTGAAGATAATGCCACTAGGCCTTGAATACCTTTCTAATCTACAATTTATGGGCTTGGCATTTGTTCCCGTTGAAGTTATAAAGCCCATTCAGAAAGGAGGTGCGGATCGGTCTAAGGTACAACACATCCCTGAGATCTTACATGTTTTCGAACAGTCATTCAAGCGGGTCTACGAAAACTTGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

920

Amino Acids

105.44

Weight (kDa)

7.15

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 94 9.9e-23 Rx N-terminal domain
NB-ARC PF00931 172 - 348 2.6e-46 NB-ARC domain
WHD_DRP PF23559 435 - 506 2.5e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 553 - 873 4.1e-37 Leucine-rich repeat region
LRR_8 PF13855 579 - 632 4.3e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2654
AasI GACNNNNNNGTC 1 cut(s) 2159
Acc36I ACCTGC 1 cut(s) 1950
AccB7I CCANNNNNTGG 1 cut(s) 1093
AccI GTMKAC 4 cut(s) 271, 1554, 2287, 2745
AciI CCGC 7 cut(s) 143, 464, 1867, 2258, 2452, 2678, 2740
AclWI GGATC 2 cut(s) 1394, 2688
AcoI YGGCCR 2 cut(s) 1546, 1640
AcsI RAATTY 7 cut(s) 233, 725, 760, 1540, 1629, 1816, 2491
AcuI CTGAAG 2 cut(s) 453, 1380
AcyI GRCGYC 1 cut(s) 1861
AfaI GTAC 3 cut(s) 1828, 2373, 2694
AfiI CCNNNNNNNGG 5 cut(s) 416, 777, 1093, 1117, 1643
AflIII ACRYGT 4 cut(s) 846, 1375, 1686, 2716
AhlI ACTAGT 1 cut(s) 799
AjiI CACGTC 1 cut(s) 847
AjnI CCWGG 1 cut(s) 828
AloI GAACNNNNNNTCC 2 cut(s) 1416, 1448
Alw21I GWGCWC 2 cut(s) 1017, 1050
Alw26I GTCTC 4 cut(s) 192, 637, 1868, 2167
AlwI GGATC 2 cut(s) 1394, 2688
AoxI GGCC 8 cut(s) 3, 147, 204, 406, 1108, 1546, 1640, 2593
ApeKI GCWGC 4 cut(s) 14, 611, 944, 1174
ApoI RAATTY 7 cut(s) 233, 725, 760, 1540, 1629, 1816, 2491
ArsI GACNNNNNNTTYG 4 cut(s) 253, 285, 690, 722
AseI ATTAAT 1 cut(s) 26
Asp700I GAANNNNTTC 4 cut(s) 128, 438, 1110, 2226
AspLEI GCGC 1 cut(s) 2014
AspS9I GGNCC 1 cut(s) 407
AsuC2I CCSGG 2 cut(s) 1644, 2546
AsuHPI GGTGA 4 cut(s) 649, 968, 2245, 2515
AsuII TTCGAA 2 cut(s) 709, 2724
BalI TGGCCA 1 cut(s) 1548
BanII GRGCYC 1 cut(s) 1017
BarI GAAGNNNNNNTAC 2 cut(s) 2355, 2387
BbsI GAAGAC 5 cut(s) 462, 495, 1140, 1174, 1480
Bbv12I GWGCWC 2 cut(s) 1017, 1050
BbvI GCAGC 4 cut(s) 26, 598, 956, 1186
BccI CCATC 4 cut(s) 528, 1276, 1403, 2023
BcgI CGANNNNNNTGC 2 cut(s) 1385, 1419
BciT130I CCWGG 1 cut(s) 830
BciVI GTATCC 1 cut(s) 2068
BclI TGATCA 1 cut(s) 717
BcnI CCSGG 2 cut(s) 1644, 2546
BcoDI GTCTC 4 cut(s) 192, 637, 1868, 2167
BcuI ACTAGT 1 cut(s) 799
BfmI CTRYAG 3 cut(s) 12, 411, 2517
BfoI RGCGCY 1 cut(s) 2015
BfuAI ACCTGC 1 cut(s) 1950
BfuI GTATCC 1 cut(s) 2068
BglII AGATCT 1 cut(s) 2709
BisI GCNGC 4 cut(s) 15, 612, 945, 1175
BlsI GCNGC 4 cut(s) 16, 613, 946, 1176
Bme1390I CCNGG 3 cut(s) 830, 1644, 2546
BmgBI CACGTC 1 cut(s) 847
BmgT120I GGNCC 1 cut(s) 407
BmiI GGNNCC 1 cut(s) 1975
BmrFI CCNGG 3 cut(s) 830, 1644, 2546
BmsI GCATC 6 cut(s) 130, 201, 1482, 1494, 1771, 2523
BpiI GAAGAC 5 cut(s) 462, 495, 1140, 1174, 1480
BpmI CTGGAG 1 cut(s) 2492
Bpu14I TTCGAA 2 cut(s) 709, 2724
BpuEI CTTGAG 1 cut(s) 1825
BpuMI CCSGG 2 cut(s) 1644, 2546
BsaBI GATNNNNATC 2 cut(s) 549, 1413
BsaHI GRCGYC 1 cut(s) 1861
BsaI GGTCTC 1 cut(s) 2167
BsaJI CCNNGG 3 cut(s) 1637, 2306, 2499
BsaWI WCCGGW 1 cut(s) 2230
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 5 cut(s) 416, 777, 1093, 1117, 1643
Bse1I ACTGG 5 cut(s) 75, 501, 1719, 1975, 2354
Bse3DI GCAATG 3 cut(s) 1217, 1282, 2051
Bse8I GATNNNNATC 2 cut(s) 549, 1413
BseBI CCWGG 1 cut(s) 830
BseDI CCNNGG 3 cut(s) 1637, 2306, 2499
BseGI GGATG 5 cut(s) 145, 539, 550, 1762, 2700
BseJI GATNNNNATC 2 cut(s) 549, 1413
BseLI CCNNNNNNNGG 5 cut(s) 416, 777, 1093, 1117, 1643
BseMI GCAATG 3 cut(s) 1217, 1282, 2051
BseMII CTCAG 3 cut(s) 1241, 2122, 2697
BseNI ACTGG 5 cut(s) 75, 501, 1719, 1975, 2354
BseRI GAGGAG 2 cut(s) 432, 1638
BseXI GCAGC 4 cut(s) 26, 598, 956, 1186
BsgI GTGCAG 2 cut(s) 473, 2330
BshFI GGCC 8 cut(s) 5, 149, 206, 408, 1110, 1548, 1642, 2595
BsiHKAI GWGCWC 2 cut(s) 1017, 1050
BsiSI CCGG 3 cut(s) 1643, 2231, 2545
BslFI GGGAC 4 cut(s) 711, 737, 2017, 2561
BslI CCNNNNNNNGG 5 cut(s) 416, 777, 1093, 1117, 1643
BsmAI GTCTC 4 cut(s) 192, 637, 1868, 2167
BsmBI CGTCTC 1 cut(s) 1868
BsmFI GGGAC 4 cut(s) 711, 737, 2017, 2561
BsmI GAATGC 1 cut(s) 1031
BsnI GGCC 8 cut(s) 5, 149, 206, 408, 1110, 1548, 1642, 2595
Bso31I GGTCTC 1 cut(s) 2167
Bsp119I TTCGAA 2 cut(s) 709, 2724
Bsp1286I GDGCHC 2 cut(s) 1017, 1050
Bsp143I GATC 9 cut(s) 366, 550, 717, 1165, 1261, 1386, 1498, 2680, 2709
Bsp19I CCATGG 1 cut(s) 1637
BspACI CCGC 7 cut(s) 143, 464, 1867, 2258, 2452, 2678, 2740
BspANI GGCC 8 cut(s) 5, 149, 206, 408, 1110, 1548, 1642, 2595
BspCNI CTCAG 3 cut(s) 1242, 2121, 2698
BspHI TCATGA 1 cut(s) 688
BspLI GGNNCC 1 cut(s) 1975
BspMAI CTGCAG 1 cut(s) 16
BspMI ACCTGC 1 cut(s) 1950
BspPI GGATC 2 cut(s) 1394, 2688
BspQI GCTCTTC 1 cut(s) 1022
BspT104I TTCGAA 2 cut(s) 709, 2724
BspTNI GGTCTC 1 cut(s) 2167
BsrDI GCAATG 3 cut(s) 1217, 1282, 2051
BsrI ACTGG 5 cut(s) 75, 501, 1719, 1975, 2354
BssECI CCNNGG 3 cut(s) 1637, 2306, 2499
BssMI GATC 9 cut(s) 366, 550, 717, 1165, 1261, 1386, 1498, 2680, 2709
BssNAI GTATAC 1 cut(s) 1555
BssNI GRCGYC 1 cut(s) 1861
BssT1I CCWWGG 2 cut(s) 1637, 2306
Bst1107I GTATAC 1 cut(s) 1555
Bst2UI CCWGG 1 cut(s) 830
Bst4CI ACNGT 4 cut(s) 565, 673, 2565, 2730
Bst6I CTCTTC 6 cut(s) 635, 1022, 1572, 1671, 2211, 2433
BstACI GRCGYC 1 cut(s) 1861
BstBI TTCGAA 2 cut(s) 709, 2724
BstC8I GCNNGC 3 cut(s) 190, 1084, 1515
BstDEI CTNAG 4 cut(s) 1250, 2108, 2688, 2706
BstDSI CCRYGG 1 cut(s) 1637
BstENI CCTNNNNNAGG 1 cut(s) 414
BstF5I GGATG 5 cut(s) 145, 539, 550, 1762, 2700
BstH2I RGCGCY 1 cut(s) 2015
BstHHI GCGC 1 cut(s) 2014
BstKTI GATC 9 cut(s) 369, 553, 720, 1168, 1264, 1389, 1501, 2683, 2712
BstMAI GTCTC 4 cut(s) 192, 637, 1868, 2167
BstMBI GATC 9 cut(s) 366, 550, 717, 1165, 1261, 1386, 1498, 2680, 2709
BstMWI GCNNNNNNNGC 4 cut(s) 11, 155, 256, 1437
BstNI CCWGG 1 cut(s) 830
BstNSI RCATGY 5 cut(s) 250, 1379, 1444, 1690, 2720
BstSCI CCNGG 3 cut(s) 828, 1642, 2544
BstSFI CTRYAG 3 cut(s) 12, 411, 2517
BstV1I GCAGC 4 cut(s) 26, 598, 956, 1186
BstV2I GAAGAC 5 cut(s) 462, 495, 1140, 1174, 1480
BstX2I RGATCY 1 cut(s) 2709
BstYI RGATCY 1 cut(s) 2709
BstZ17I GTATAC 1 cut(s) 1555
BsuI GTATCC 1 cut(s) 2068
BsuRI GGCC 8 cut(s) 5, 149, 206, 408, 1110, 1548, 1642, 2595
BtgI CCRYGG 1 cut(s) 1637
BtrI CACGTC 1 cut(s) 847
BtsCI GGATG 5 cut(s) 145, 539, 550, 1762, 2700
BtsI GCAGTG 2 cut(s) 2318, 2390
BtsIMutI CAGTG 6 cut(s) 82, 406, 1712, 2318, 2347, 2390
BveI ACCTGC 1 cut(s) 1950
Cac8I GCNNGC 3 cut(s) 190, 1084, 1515
CciI TCATGA 1 cut(s) 688
CfoI GCGC 1 cut(s) 2014
Cfr13I GGNCC 1 cut(s) 407
CseI GACGC 1 cut(s) 1850
CsiI ACCWGGT 1 cut(s) 828
Csp6I GTAC 3 cut(s) 1827, 2372, 2693
CviQI GTAC 3 cut(s) 1827, 2372, 2693
DdeI CTNAG 4 cut(s) 1250, 2108, 2688, 2706
DpnI GATC 9 cut(s) 368, 552, 719, 1167, 1263, 1388, 1500, 2682, 2711
DpnII GATC 9 cut(s) 366, 550, 717, 1165, 1261, 1386, 1498, 2680, 2709
DraI TTTAAA 1 cut(s) 1815
DrdI GACNNNNNNGTC 1 cut(s) 2159
DseDI GACNNNNNNGTC 1 cut(s) 2159
EaeI YGGCCR 2 cut(s) 1546, 1640
Eam1104I CTCTTC 6 cut(s) 635, 1022, 1572, 1671, 2211, 2433
EarI CTCTTC 6 cut(s) 635, 1022, 1572, 1671, 2211, 2433
Ecl136II GAGCTC 1 cut(s) 1015
Eco130I CCWWGG 2 cut(s) 1637, 2306
Eco147I AGGCCT 3 cut(s) 149, 1110, 2595
Eco24I GRGCYC 1 cut(s) 1017
Eco31I GGTCTC 1 cut(s) 2167
Eco32I GATATC 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 1015
Eco57I CTGAAG 2 cut(s) 453, 1380
EcoICRI GAGCTC 1 cut(s) 1015
EcoNI CCTNNNNNAGG 1 cut(s) 414
EcoRI GAATTC 2 cut(s) 233, 725
EcoRII CCWGG 1 cut(s) 828
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 2 cut(s) 1637, 2306
EcoT22I ATGCAT 3 cut(s) 252, 1497, 2428
EcoT38I GRGCYC 1 cut(s) 1017
ErhI CCWWGG 2 cut(s) 1637, 2306
Esp3I CGTCTC 1 cut(s) 1868
FaqI GGGAC 4 cut(s) 711, 737, 2017, 2561
FauI CCCGC 1 cut(s) 2733
FauNDI CATATG 2 cut(s) 1203, 2428
FbaI TGATCA 1 cut(s) 717
FblI GTMKAC 4 cut(s) 271, 1554, 2287, 2745
Fnu4HI GCNGC 4 cut(s) 15, 612, 945, 1175
FokI GGATG 5 cut(s) 152, 546, 557, 1769, 2687
FriOI GRGCYC 1 cut(s) 1017
Fsp4HI GCNGC 4 cut(s) 15, 612, 945, 1175
GlaI GCGC 1 cut(s) 2013
GluI GCNGC 4 cut(s) 15, 612, 945, 1175
GsuI CTGGAG 1 cut(s) 2492
HaeII RGCGCY 1 cut(s) 2015
HaeIII GGCC 8 cut(s) 5, 149, 206, 408, 1110, 1548, 1642, 2595
HapII CCGG 3 cut(s) 1643, 2231, 2545
HgaI GACGC 1 cut(s) 1850
HhaI GCGC 1 cut(s) 2014
Hin1I GRCGYC 1 cut(s) 1861
Hin6I GCGC 1 cut(s) 2012
HinP1I GCGC 1 cut(s) 2012
HincII GTYRAC 4 cut(s) 272, 805, 1968, 2178
HindII GTYRAC 4 cut(s) 272, 805, 1968, 2178
HindIII AAGCTT 3 cut(s) 126, 712, 2270
HpaI GTTAAC 1 cut(s) 805
HpaII CCGG 3 cut(s) 1643, 2231, 2545
HphI GGTGA 4 cut(s) 649, 968, 2245, 2515
Hpy188I TCNGA 7 cut(s) 197, 382, 433, 905, 1503, 2068, 2667
Hpy99I CGWCG 2 cut(s) 1562, 1596
HpyCH4III ACNGT 4 cut(s) 565, 673, 2565, 2730
HpyCH4IV ACGT 2 cut(s) 328, 846
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 155, 256, 1437
HpyF3I CTNAG 4 cut(s) 1250, 2108, 2688, 2706
HpySE526I ACGT 2 cut(s) 328, 846
Hsp92I GRCGYC 1 cut(s) 1861
HspAI GCGC 1 cut(s) 2012
Ksp22I TGATCA 1 cut(s) 717
KspAI GTTAAC 1 cut(s) 805
Kzo9I GATC 9 cut(s) 366, 550, 717, 1165, 1261, 1386, 1498, 2680, 2709
LguI GCTCTTC 1 cut(s) 1022
LmnI GCTCC 3 cut(s) 109, 419, 1012
Lsp1109I GCAGC 4 cut(s) 26, 598, 956, 1186
LweI GCATC 6 cut(s) 130, 201, 1482, 1494, 1771, 2523
MabI ACCWGGT 1 cut(s) 828
MaeII ACGT 2 cut(s) 328, 846
MaeIII GTNAC 5 cut(s) 345, 795, 919, 1443, 2031
MalI GATC 9 cut(s) 368, 552, 719, 1167, 1263, 1388, 1500, 2682, 2711
MboI GATC 9 cut(s) 366, 550, 717, 1165, 1261, 1386, 1498, 2680, 2709
MfeI CAATTG 3 cut(s) 1274, 2166, 2276
MflI RGATCY 1 cut(s) 2709
MhlI GDGCHC 2 cut(s) 1017, 1050
MlsI TGGCCA 1 cut(s) 1548
MluNI TGGCCA 1 cut(s) 1548
MlyI GAGTC 7 cut(s) 277, 397, 476, 824, 1735, 2095, 2173
Mox20I TGGCCA 1 cut(s) 1548
Mph1103I ATGCAT 3 cut(s) 252, 1497, 2428
MroXI GAANNNNTTC 4 cut(s) 128, 438, 1110, 2226
MscI TGGCCA 1 cut(s) 1548
MseI TTAA 9 cut(s) 26, 804, 963, 1488, 1706, 1814, 1832, 2201, 2552
MslI CAYNNNNRTG 2 cut(s) 249, 657
Msp20I TGGCCA 1 cut(s) 1548
MspI CCGG 3 cut(s) 1643, 2231, 2545
MspR9I CCNGG 3 cut(s) 830, 1644, 2546
MunI CAATTG 3 cut(s) 1274, 2166, 2276
Mva1269I GAATGC 1 cut(s) 1031
MvaI CCWGG 1 cut(s) 830
MwoI GCNNNNNNNGC 4 cut(s) 11, 155, 256, 1437
NciI CCSGG 2 cut(s) 1644, 2546
NcoI CCATGG 1 cut(s) 1637
NdeI CATATG 2 cut(s) 1203, 2428
NdeII GATC 9 cut(s) 366, 550, 717, 1165, 1261, 1386, 1498, 2680, 2709
NlaIV GGNNCC 1 cut(s) 1975
NmeAIII GCCGAG 1 cut(s) 1555
NsiI ATGCAT 3 cut(s) 252, 1497, 2428
NspI RCATGY 5 cut(s) 250, 1379, 1444, 1690, 2720
NspV TTCGAA 2 cut(s) 709, 2724
PagI TCATGA 1 cut(s) 688
PceI AGGCCT 3 cut(s) 149, 1110, 2595
PciI ACATGT 3 cut(s) 1375, 1686, 2716
PciSI GCTCTTC 1 cut(s) 1022
PctI GAATGC 1 cut(s) 1031
PdmI GAANNNNTTC 4 cut(s) 128, 438, 1110, 2226
PfeI GAWTC 3 cut(s) 377, 1657, 2063
PflMI CCANNNNNTGG 1 cut(s) 1093
PfoI TCCNGGA 1 cut(s) 2544
PkrI GCNGC 4 cut(s) 16, 613, 946, 1176
PleI GAGTC 7 cut(s) 276, 396, 475, 823, 1735, 2095, 2173
PpsI GAGTC 7 cut(s) 276, 396, 475, 823, 1735, 2095, 2173
PscI ACATGT 3 cut(s) 1375, 1686, 2716
PshBI ATTAAT 1 cut(s) 26
PsiI TTATAA 1 cut(s) 2654
Psp124BI GAGCTC 1 cut(s) 1017
Psp6I CCWGG 1 cut(s) 828
PspGI CCWGG 1 cut(s) 828
PspN4I GGNNCC 1 cut(s) 1975
PspPI GGNCC 1 cut(s) 407
PstI CTGCAG 1 cut(s) 16
PsuI RGATCY 1 cut(s) 2709
RsaI GTAC 3 cut(s) 1828, 2373, 2694
RsaNI GTAC 3 cut(s) 1827, 2372, 2693
RseI CAYNNNNRTG 2 cut(s) 249, 657
SacI GAGCTC 1 cut(s) 1017
SalI GTCGAC 1 cut(s) 270
SapI GCTCTTC 1 cut(s) 1022
SaqAI TTAA 9 cut(s) 26, 804, 963, 1488, 1706, 1814, 1832, 2201, 2552
SatI GCNGC 4 cut(s) 15, 612, 945, 1175
Sau3AI GATC 9 cut(s) 366, 550, 717, 1165, 1261, 1386, 1498, 2680, 2709
Sau96I GGNCC 1 cut(s) 407
SchI GAGTC 7 cut(s) 277, 397, 476, 824, 1735, 2095, 2173
ScrFI CCNGG 3 cut(s) 830, 1644, 2546
SduI GDGCHC 2 cut(s) 1017, 1050
SexAI ACCWGGT 1 cut(s) 828
SfaNI GCATC 6 cut(s) 130, 201, 1482, 1494, 1771, 2523
SfcI CTRYAG 3 cut(s) 12, 411, 2517
SfuI TTCGAA 2 cut(s) 709, 2724
SmiI ATTTAAAT 1 cut(s) 1815
SmiMI CAYNNNNRTG 2 cut(s) 249, 657
SmlI CTYRAG 1 cut(s) 1804
SmoI CTYRAG 1 cut(s) 1804
SpeI ACTAGT 1 cut(s) 799
SseBI AGGCCT 3 cut(s) 149, 1110, 2595
SsiI CCGC 7 cut(s) 143, 464, 1867, 2258, 2452, 2678, 2740
SspI AATATT 2 cut(s) 1880, 2023
SstI GAGCTC 1 cut(s) 1017
StuI AGGCCT 3 cut(s) 149, 1110, 2595
StyD4I CCNGG 3 cut(s) 828, 1642, 2544
StyI CCWWGG 2 cut(s) 1637, 2306
SwaI ATTTAAAT 1 cut(s) 1815
TaaI ACNGT 4 cut(s) 565, 673, 2565, 2730
TaiI ACGT 2 cut(s) 331, 849
TaqI TCGA 9 cut(s) 271, 709, 928, 1371, 1523, 1723, 1890, 2126, 2724
TaqII GACCGA 2 cut(s) 1492, 2673
TatI WGTACW 1 cut(s) 2371
TfiI GAWTC 3 cut(s) 377, 1657, 2063
Tru1I TTAA 9 cut(s) 26, 804, 963, 1488, 1706, 1814, 1832, 2201, 2552
Tru9I TTAA 9 cut(s) 26, 804, 963, 1488, 1706, 1814, 1832, 2201, 2552
TscAI CASTG 6 cut(s) 82, 406, 1719, 2325, 2354, 2397
TseI GCWGC 4 cut(s) 14, 611, 944, 1174
TspGWI ACGGA 1 cut(s) 1424
TspRI CASTG 6 cut(s) 82, 406, 1719, 2325, 2354, 2397
Van91I CCANNNNNTGG 1 cut(s) 1093
VspI ATTAAT 1 cut(s) 26
XagI CCTNNNNNAGG 1 cut(s) 414
XapI RAATTY 7 cut(s) 233, 725, 760, 1540, 1629, 1816, 2491
XbaI TCTAGA 2 cut(s) 838, 1744
XceI RCATGY 5 cut(s) 250, 1379, 1444, 1690, 2720
XcmI CCANNNNNNNNNTGG 2 cut(s) 315, 826
XmiI GTMKAC 4 cut(s) 271, 1554, 2287, 2745
XmnI GAANNNNTTC 4 cut(s) 128, 438, 1110, 2226
Zsp2I ATGCAT 3 cut(s) 252, 1497, 2428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.