Rw5G010460

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000110
Physical Location & Seq
Forward (+)
11491600 .. 11494568
2969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G010460.1

Sequence Viewer

Length: 2691 bp
ATGGCTTCATCTGCAACGGACCTCTTAATTGGAAAAATTGTGGCAATTCTTGAGAACGAAGGAACGACCATAGCCAGTATTTGGGATGAAGTTGATAAGATTAAGCAGGAGTTTTTGAGCATGAAAGCTTTCCTTGCGGATGCAGAGACCAACACAGCAAAAACGCAAGGAGAGAAATTGTGGGTTGCAAGCATCAGAGACTTGGCCTATGATGTTGAAGATATCATCGATGAATTCATGTATCATATGTATGAGAAGCAAACTGGGGGTCGACTTTCAAGGGGGCTACACAAAACCATTCGCACTCCAACTAGTCTTTGGTTCAGGCATAAAATTGCAAAGAAGTTGCAGAAGATTACTGAAATAATCAAAGCCATTCCAGAGAGGAATCAAAGGTACGGTGTTGGCCTTGTTGGAGGAGCAACTACATGTGAAGATATTTACAAATTGGTGCAGAACCAAGCCGAGTCCTCTATTTTCATTATGGAAGACGGACTGGTTGGGATTGAAGGCAAGAAGCAAATACTAATGGAATGGCTCATGGATGAAGAGCAACACCAAACTGTTATATCTGTGGTTGGGATGGGTGGTTCTGGAAAGACAACTCTAAGACATTTCGACTGTTGTGCATGGATTACCGTTTCCCAAACTTATGCTGTGGCAGACTTGTTCCGAAGCTTGATCATAGAACTCCACAAATCAAGGCAGGAAGAGTACCCTGCAAACTTAAATGCCATGAGCCGCATAGAATTGCTAGAGTTACTGACAAACTACTTGGAGTCTAAAAAATACCTAGTTGTTCTAGATGATGTGTGGGAGATAAGAGTTTGGAGAGAAATAAAGGTGTCACTTCAAGATAGACAACTCGGAAGTCGAATAGTACTTACAACTCGAAATGAAGAAGTAGCATCCAACTCTTTTGGAGTTGAAAGCTATGTTCACCATATTCAGCCCCTGCACAATAATGAGGCTTGGGAACTTTTCCGCAAGAAAGCATTCTCCACTAATCAGCACAAAACTTGTCCGCTTGAGCTTGAGTCATTGGCTTGCCAACTTGTGGAAAAGTGTGGAGGCCTTCCTTTGGCTATTGTGGCTTTAGGTGGTCTTATGTCTTCCAAGAAGTCCCTGGATCAATGGCAACGAGTCTGCAACAGCTTAAACTGGCATCTAGATAACAATTCATTGCTAGACCCTGTGAAAAACATCTTGTTGCTGAGTTTCAATGATTTGCCATCTCAACTGAAGCATTGCTTCCTCTATTGCTCTCTATTCCCAGAAGATTTTGTAATTAAGAGAAACAGGCTCATTAGATTGTGGATAGCCGAAGGATTTGTTGAACATGTGAAAGGGATCACACCAGAAGATGTTGCAGATGAATATCTTATGGAGCTCTGTTTCCGTAGCATGTTACAAGTTGAGAGACATGATACGGGAAGGTCGAAACAAGTTAAGATGCATGATCTGATGCGAGAGCTTGCTTTGTCGACAGCCGAGAAGGAAAAGTTTGGTCTTATATACGACGGGAGAGAAGGAATGAAAGACATCTCAACCCGTCGCTTGTCAATTCACAAAATGGAAGGAGAAATTAAATCATGGCCGGGTATGTCAAAGATTCGTTCTCTTCTTGTCTTTGCAACTGACATGTCCTCATTGTCTTTCTCAAATGCACTGGTTTCTCGATTCAAATTGTTAAGGACTCTAGACTTGGAGGATGTCCAAATTGATAAACTGCCGGGTGCAGTTGTTCACTTGTTCAACTTGAGATATTTAAATTTGAAGGGCACTTTAATCGAGAAACTTCCAAAGTCAATAGAGCGTCTCCGCAACCTTCAATTTCTAAACATCAAGCATACTAAGATAGATTCACTTCCACAAGGAATTACGAAGTTGCTAAGCCTGCGACATCTAATATTTGCGAGTGGGACAAAAGTGCCATCAAATATTTGTCAGTTGCAGAAATTGCAATGTTTATCAATGATTGAATCAGAAGGGGACACTGGTAAACTCATCGGCAACATGACTCAACTTAGGTGCATCGGCATATCGAATCTGAAAGAAAGAGATGAGATTGACCTTTGGGCCTCAATTCAAAAGTTGACTCTGCTTCAATCTATGGGTTTAATGGCAAGTAATGAAGAGGAAATTCTTCCGGTGAAAGCAAAATGTCCACCTCTTCCGCACCTTCAAAAGCTTTATTTGATTGGCAAACTGCAAAACGGAATACCTTGGTTTTCTTCATTGCATAGCCTCACAACTCTGTTTTTGCTTTGGTCAAGACTTGAAGAGGATTTCCTACCTCAAATTGAAGCACTGCCCAATTTAACAAGGCTTGTTGTTAATAAGGCATATGTTGGGGAAGAGTTGTGTTTCCGCAGAGGTTTTGTAAAGCTGGAGAGTTTGAAGTTGTCGAATTTTGCCTCGATGAAAAAGATAACTATAGAAAAAGGGGTGATGCCAAAACTCCGGTACTTATGGGTTGAACACTGCACGGAATTGAAGACATTGCCATTGGGCCTTGAATACCTTTCTAATCTACAAACTTTGAGCCTGGAAGATGTTCCCATGGAAGTTGTAAGGCCTATTCAGAAAGAAGGTGTGGATCGCTTTAAGGTACAACACATCCTTGAGATTAAACATTTTTTCCTACGATCATCCAAGCGGTCTGAGAAGTATATATTTAAGAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

896

Amino Acids

102.71

Weight (kDa)

8.46

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 92 8.5e-20 Rx N-terminal domain
NB-ARC PF00931 172 - 336 5.2e-38 NB-ARC domain
WHD_DRP PF23559 423 - 493 2.2e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 537 - 850 4.4e-44 Leucine-rich repeat region
LRR_8 PF13855 564 - 620 1.7e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 81, 1057
AccI GTMKAC 2 cut(s) 271, 1484
AciI CCGC 8 cut(s) 137, 742, 985, 1025, 1822, 2177, 2371, 2659
AclWI GGATC 3 cut(s) 1137, 1358, 2607
AcoI YGGCCR 1 cut(s) 1595
AcsI RAATTY 4 cut(s) 233, 1771, 2142, 2410
AcuI CTGAAG 1 cut(s) 1262
AfaI GTAC 5 cut(s) 398, 716, 882, 2468, 2613
AfiI CCNNNNNNNGG 3 cut(s) 81, 1057, 1081
AflIII ACRYGT 3 cut(s) 428, 1339, 1641
AhlI ACTAGT 1 cut(s) 311
AjnI CCWGG 2 cut(s) 1125, 2547
AloI GAACNNNNNNTCC 2 cut(s) 574, 606
AluBI AGCT 9 cut(s) 128, 678, 933, 1033, 1155, 1390, 1474, 2191, 2389
AluI AGCT 9 cut(s) 128, 678, 933, 1033, 1155, 1390, 1474, 2191, 2389
Alw21I GWGCWC 1 cut(s) 1392
Alw26I GTCTC 4 cut(s) 140, 192, 1414, 1823
AlwI GGATC 3 cut(s) 1137, 1358, 2607
AlwNI CAGNNNCTG 1 cut(s) 955
AoxI GGCC 7 cut(s) 204, 406, 1072, 1595, 2079, 2512, 2576
ApoI RAATTY 4 cut(s) 233, 1771, 2142, 2410
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 4 cut(s) 128, 995, 2145, 2556
AspS9I GGNCC 3 cut(s) 19, 2079, 2512
AsuC2I CCSGG 2 cut(s) 1599, 1734
AsuHPI GGTGA 3 cut(s) 932, 2164, 2461
AvaII GGWCC 1 cut(s) 19
BaeGI GKGCMC 1 cut(s) 1784
BanII GRGCYC 1 cut(s) 1392
BbsI GAAGAC 3 cut(s) 495, 1104, 2504
Bbv12I GWGCWC 1 cut(s) 1392
BccI CCATC 3 cut(s) 577, 1240, 1942
BcgI CGANNNNNNTGC 4 cut(s) 608, 642, 1771, 1805
BciT130I CCWGG 2 cut(s) 1127, 2549
BclI TGATCA 1 cut(s) 681
BcnI CCSGG 2 cut(s) 1599, 1734
BcoDI GTCTC 4 cut(s) 140, 192, 1414, 1823
BcuI ACTAGT 1 cut(s) 311
BfaI CTAG 7 cut(s) 312, 755, 794, 803, 1169, 1187, 1700
BfmI CTRYAG 1 cut(s) 2436
BisI GCNGC 1 cut(s) 742
BlpI GCTNAGC 1 cut(s) 1892
BlsI GCNGC 1 cut(s) 743
BmcAI AGTACT 1 cut(s) 882
Bme1390I CCNGG 4 cut(s) 1127, 1599, 1734, 2549
Bme18I GGWCC 1 cut(s) 19
BmgT120I GGNCC 3 cut(s) 19, 2079, 2512
BmrFI CCNGG 4 cut(s) 1127, 1599, 1734, 2549
BmrI ACTGGG 1 cut(s) 273
BmsI GCATC 8 cut(s) 130, 201, 917, 1174, 1443, 1455, 2043, 2442
BmuI ACTGGG 1 cut(s) 273
BpiI GAAGAC 3 cut(s) 495, 1104, 2504
BpmI CTGGAG 1 cut(s) 2411
Bpu1102I GCTNAGC 1 cut(s) 1892
BpuEI CTTGAG 5 cut(s) 71, 1049, 1055, 1780, 2645
BpuMI CCSGG 2 cut(s) 1599, 1734
Bsa29I ATCGAT 1 cut(s) 228
BsaBI GATNNNNATC 1 cut(s) 1377
BsaI GGTCTC 1 cut(s) 140
BsaJI CCNNGG 3 cut(s) 1125, 2225, 2562
BsaWI WCCGGW 2 cut(s) 2149, 2463
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 1057, 1081
Bse1I ACTGG 6 cut(s) 75, 268, 501, 1166, 1674, 2002
Bse3DI GCAATG 5 cut(s) 1181, 1246, 1970, 2237, 2501
Bse8I GATNNNNATC 1 cut(s) 1377
BseBI CCWGG 2 cut(s) 1127, 2549
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 3 cut(s) 1125, 2225, 2562
BseGI GGATG 8 cut(s) 91, 145, 550, 588, 908, 1717, 2619, 2651
BseJI GATNNNNATC 1 cut(s) 1377
BseLI CCNNNNNNNGG 3 cut(s) 81, 1057, 1081
BseMI GCAATG 5 cut(s) 1181, 1246, 1970, 2237, 2501
BseMII CTCAG 2 cut(s) 1205, 2655
BseNI ACTGG 6 cut(s) 75, 268, 501, 1166, 1674, 2002
BseRI GAGGAG 1 cut(s) 432
BseSI GKGCMC 1 cut(s) 1784
BsgI GTGCAG 4 cut(s) 473, 941, 1758, 2470
BshFI GGCC 7 cut(s) 206, 408, 1074, 1597, 2081, 2514, 2578
BshVI ATCGAT 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 1392
BsiSI CCGG 4 cut(s) 1598, 1733, 2150, 2464
BslFI GGGAC 3 cut(s) 1108, 1936, 2006
BslI CCNNNNNNNGG 3 cut(s) 81, 1057, 1081
BsmAI GTCTC 4 cut(s) 140, 192, 1414, 1823
BsmBI CGTCTC 1 cut(s) 1823
BsmFI GGGAC 3 cut(s) 1108, 1936, 2006
BsmI GAATGC 1 cut(s) 995
BsnI GGCC 7 cut(s) 206, 408, 1074, 1597, 2081, 2514, 2578
Bso31I GGTCTC 1 cut(s) 140
Bsp1286I GDGCHC 2 cut(s) 1392, 1784
Bsp143I GATC 6 cut(s) 681, 1129, 1350, 1459, 2599, 2648
Bsp1720I GCTNAGC 1 cut(s) 1892
Bsp19I CCATGG 1 cut(s) 2562
BspACI CCGC 8 cut(s) 137, 742, 985, 1025, 1822, 2177, 2371, 2659
BspANI GGCC 7 cut(s) 206, 408, 1074, 1597, 2081, 2514, 2578
BspCNI CTCAG 2 cut(s) 1206, 2656
BspDI ATCGAT 1 cut(s) 228
BspPI GGATC 3 cut(s) 1137, 1358, 2607
BspQI GCTCTTC 1 cut(s) 543
BspTNI GGTCTC 1 cut(s) 140
BsrDI GCAATG 5 cut(s) 1181, 1246, 1970, 2237, 2501
BsrI ACTGG 6 cut(s) 75, 268, 501, 1166, 1674, 2002
BssECI CCNNGG 3 cut(s) 1125, 2225, 2562
BssMI GATC 6 cut(s) 681, 1129, 1350, 1459, 2599, 2648
BssT1I CCWWGG 2 cut(s) 2225, 2562
Bst2UI CCWGG 2 cut(s) 1127, 2549
Bst4CI ACNGT 4 cut(s) 401, 565, 623, 640
Bst6I CTCTTC 7 cut(s) 543, 705, 1626, 2130, 2178, 2277, 2352
BstAPI GCANNNNNTGC 1 cut(s) 1960
BstC8I GCNNGC 4 cut(s) 190, 1048, 1476, 1898
BstDEI CTNAG 6 cut(s) 608, 1214, 1854, 1892, 2027, 2664
BstDSI CCRYGG 1 cut(s) 2562
BstF5I GGATG 8 cut(s) 91, 145, 550, 588, 908, 1717, 2619, 2651
BstKTI GATC 6 cut(s) 684, 1132, 1353, 1462, 2602, 2651
BstMAI GTCTC 4 cut(s) 140, 192, 1414, 1823
BstMBI GATC 6 cut(s) 681, 1129, 1350, 1459, 2599, 2648
BstMWI GCNNNNNNNGC 5 cut(s) 11, 134, 1091, 1897, 1960
BstNI CCWGG 2 cut(s) 1127, 2549
BstNSI RCATGY 4 cut(s) 432, 1343, 1408, 1645
BstSCI CCNGG 4 cut(s) 1125, 1597, 1732, 2547
BstSFI CTRYAG 1 cut(s) 2436
BstSLI GKGCMC 1 cut(s) 1784
BstV2I GAAGAC 3 cut(s) 495, 1104, 2504
Bsu15I ATCGAT 1 cut(s) 228
BsuRI GGCC 7 cut(s) 206, 408, 1074, 1597, 2081, 2514, 2578
BsuTUI ATCGAT 1 cut(s) 228
BtgI CCRYGG 1 cut(s) 2562
BtsCI GGATG 8 cut(s) 91, 145, 550, 588, 908, 1717, 2619, 2651
BtsI GCAGTG 2 cut(s) 2309, 2482
BtsIMutI CAGTG 4 cut(s) 1667, 1995, 2309, 2482
Cac8I GCNNGC 4 cut(s) 190, 1048, 1476, 1898
CaiI CAGNNNCTG 1 cut(s) 955
Cfr13I GGNCC 3 cut(s) 19, 2079, 2512
ClaI ATCGAT 1 cut(s) 228
CseI GACGC 1 cut(s) 1805
Csp6I GTAC 5 cut(s) 397, 715, 881, 2467, 2612
CviQI GTAC 5 cut(s) 397, 715, 881, 2467, 2612
DdeI CTNAG 6 cut(s) 608, 1214, 1854, 1892, 2027, 2664
DpnI GATC 6 cut(s) 683, 1131, 1352, 1461, 2601, 2650
DpnII GATC 6 cut(s) 681, 1129, 1350, 1459, 2599, 2648
DraI TTTAAA 1 cut(s) 1770
EaeI YGGCCR 1 cut(s) 1595
Eam1104I CTCTTC 7 cut(s) 543, 705, 1626, 2130, 2178, 2277, 2352
EarI CTCTTC 7 cut(s) 543, 705, 1626, 2130, 2178, 2277, 2352
Ecl136II GAGCTC 1 cut(s) 1390
Eco130I CCWWGG 2 cut(s) 2225, 2562
Eco147I AGGCCT 2 cut(s) 1074, 2578
Eco24I GRGCYC 1 cut(s) 1392
Eco31I GGTCTC 1 cut(s) 140
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 1390
Eco57I CTGAAG 1 cut(s) 1262
EcoICRI GAGCTC 1 cut(s) 1390
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 2 cut(s) 1125, 2547
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 2 cut(s) 2225, 2562
EcoT22I ATGCAT 1 cut(s) 1458
EcoT38I GRGCYC 1 cut(s) 1392
ErhI CCWWGG 2 cut(s) 2225, 2562
Esp3I CGTCTC 1 cut(s) 1823
FalI AAGNNNNNCTT 4 cut(s) 117, 149, 1235, 1267
FaqI GGGAC 3 cut(s) 1108, 1936, 2006
FauNDI CATATG 2 cut(s) 246, 2347
FbaI TGATCA 1 cut(s) 681
FblI GTMKAC 2 cut(s) 271, 1484
Fnu4HI GCNGC 1 cut(s) 742
FokI GGATG 8 cut(s) 98, 152, 557, 595, 895, 1724, 2606, 2638
FriOI GRGCYC 1 cut(s) 1392
Fsp4HI GCNGC 1 cut(s) 742
FspBI CTAG 7 cut(s) 312, 755, 794, 803, 1169, 1187, 1700
GluI GCNGC 1 cut(s) 742
GsuI CTGGAG 1 cut(s) 2411
HaeIII GGCC 7 cut(s) 206, 408, 1074, 1597, 2081, 2514, 2578
HapII CCGG 4 cut(s) 1598, 1733, 2150, 2464
HgaI GACGC 1 cut(s) 1805
HincII GTYRAC 3 cut(s) 272, 1485, 2097
HindII GTYRAC 3 cut(s) 272, 1485, 2097
HindIII AAGCTT 3 cut(s) 126, 676, 2189
HpaII CCGG 4 cut(s) 1598, 1733, 2150, 2464
HphI GGTGA 3 cut(s) 932, 2164, 2461
Hpy166II GTNNAC 7 cut(s) 272, 940, 1485, 1747, 2003, 2097, 2168
Hpy188I TCNGA 8 cut(s) 197, 674, 869, 1464, 1987, 2052, 2586, 2665
Hpy8I GTNNAC 7 cut(s) 272, 940, 1485, 1747, 2003, 2097, 2168
Hpy99I CGWCG 2 cut(s) 1523, 1557
HpyCH4III ACNGT 4 cut(s) 401, 565, 623, 640
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 134, 1091, 1897, 1960
HpyF3I CTNAG 6 cut(s) 608, 1214, 1854, 1892, 2027, 2664
Ksp22I TGATCA 1 cut(s) 681
Kzo9I GATC 6 cut(s) 681, 1129, 1350, 1459, 2599, 2648
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 2 cut(s) 419, 1387
LweI GCATC 8 cut(s) 130, 201, 917, 1174, 1443, 1455, 2043, 2442
MaeI CTAG 7 cut(s) 312, 755, 794, 803, 1169, 1187, 1700
MaeIII GTNAC 3 cut(s) 759, 846, 1407
MalI GATC 6 cut(s) 683, 1131, 1352, 1461, 2601, 2650
MboI GATC 6 cut(s) 681, 1129, 1350, 1459, 2599, 2648
MhlI GDGCHC 2 cut(s) 1392, 1784
MlyI GAGTC 7 cut(s) 476, 788, 1046, 1152, 1690, 2014, 2092
MmeI TCCRAC 3 cut(s) 332, 394, 936
Mph1103I ATGCAT 1 cut(s) 1458
MroXI GAANNNNTTC 4 cut(s) 128, 995, 2145, 2556
MslI CAYNNNNRTG 2 cut(s) 249, 963
MspI CCGG 4 cut(s) 1598, 1733, 2150, 2464
MspR9I CCNGG 4 cut(s) 1127, 1599, 1734, 2549
Mva1269I GAATGC 1 cut(s) 995
MvaI CCWGG 2 cut(s) 1127, 2549
MwoI GCNNNNNNNGC 5 cut(s) 11, 134, 1091, 1897, 1960
NciI CCSGG 2 cut(s) 1599, 1734
NcoI CCATGG 1 cut(s) 2562
NdeI CATATG 2 cut(s) 246, 2347
NdeII GATC 6 cut(s) 681, 1129, 1350, 1459, 2599, 2648
NmeAIII GCCGAG 2 cut(s) 490, 1516
NmuCI GTSAC 1 cut(s) 846
NsiI ATGCAT 1 cut(s) 1458
NspI RCATGY 4 cut(s) 432, 1343, 1408, 1645
PceI AGGCCT 2 cut(s) 1074, 2578
PciI ACATGT 3 cut(s) 428, 1339, 1641
PciSI GCTCTTC 1 cut(s) 543
PcsI WCGNNNNNNNCGW 1 cut(s) 1436
PctI GAATGC 1 cut(s) 995
PdmI GAANNNNTTC 4 cut(s) 128, 995, 2145, 2556
PfeI GAWTC 6 cut(s) 388, 1612, 1680, 1862, 1982, 2047
PflMI CCANNNNNTGG 2 cut(s) 81, 1057
PkrI GCNGC 1 cut(s) 743
PleI GAGTC 7 cut(s) 475, 787, 1045, 1151, 1690, 2014, 2092
PpsI GAGTC 7 cut(s) 475, 787, 1045, 1151, 1690, 2014, 2092
PscI ACATGT 3 cut(s) 428, 1339, 1641
Psp124BI GAGCTC 1 cut(s) 1392
Psp6I CCWGG 2 cut(s) 1125, 2547
PspGI CCWGG 2 cut(s) 1125, 2547
PspPI GGNCC 3 cut(s) 19, 2079, 2512
PstNI CAGNNNCTG 1 cut(s) 955
RsaI GTAC 5 cut(s) 398, 716, 882, 2468, 2613
RsaNI GTAC 5 cut(s) 397, 715, 881, 2467, 2612
RseI CAYNNNNRTG 2 cut(s) 249, 963
SacI GAGCTC 1 cut(s) 1392
SalI GTCGAC 2 cut(s) 270, 1483
SapI GCTCTTC 1 cut(s) 543
SatI GCNGC 1 cut(s) 742
Sau3AI GATC 6 cut(s) 681, 1129, 1350, 1459, 2599, 2648
Sau96I GGNCC 3 cut(s) 19, 2079, 2512
ScaI AGTACT 1 cut(s) 882
SchI GAGTC 7 cut(s) 476, 788, 1046, 1152, 1690, 2014, 2092
ScrFI CCNGG 4 cut(s) 1127, 1599, 1734, 2549
SduI GDGCHC 2 cut(s) 1392, 1784
SfaNI GCATC 8 cut(s) 130, 201, 917, 1174, 1443, 1455, 2043, 2442
SfcI CTRYAG 1 cut(s) 2436
SinI GGWCC 1 cut(s) 19
SmiI ATTTAAAT 1 cut(s) 1770
SmiMI CAYNNNNRTG 2 cut(s) 249, 963
SmlI CTYRAG 5 cut(s) 50, 1028, 1034, 1759, 2624
SmoI CTYRAG 5 cut(s) 50, 1028, 1034, 1759, 2624
SpeI ACTAGT 1 cut(s) 311
SseBI AGGCCT 2 cut(s) 1074, 2578
SsiI CCGC 8 cut(s) 137, 742, 985, 1025, 1822, 2177, 2371, 2659
SspI AATATT 2 cut(s) 1911, 1942
SspMI CTAG 7 cut(s) 312, 755, 794, 803, 1169, 1187, 1700
SstI GAGCTC 1 cut(s) 1392
StuI AGGCCT 2 cut(s) 1074, 2578
StyD4I CCNGG 4 cut(s) 1125, 1597, 1732, 2547
StyI CCWWGG 2 cut(s) 2225, 2562
SwaI ATTTAAAT 1 cut(s) 1770
TaaI ACNGT 4 cut(s) 401, 565, 623, 640
TatI WGTACW 1 cut(s) 880
TauI GCSGC 1 cut(s) 744
TfiI GAWTC 6 cut(s) 388, 1612, 1680, 1862, 1982, 2047
TscAI CASTG 4 cut(s) 1674, 2002, 2316, 2489
TseFI GTSAC 1 cut(s) 846
Tsp45I GTSAC 1 cut(s) 846
TspGWI ACGGA 5 cut(s) 32, 507, 1388, 2232, 2504
TspRI CASTG 4 cut(s) 1674, 2002, 2316, 2489
Van91I CCANNNNNTGG 2 cut(s) 81, 1057
VpaK11BI GGWCC 1 cut(s) 19
XapI RAATTY 4 cut(s) 233, 1771, 2142, 2410
XbaI TCTAGA 3 cut(s) 802, 1168, 1699
XceI RCATGY 4 cut(s) 432, 1343, 1408, 1645
XcmI CCANNNNNNNNNTGG 2 cut(s) 315, 1123
XmiI GTMKAC 2 cut(s) 271, 1484
XmnI GAANNNNTTC 4 cut(s) 128, 995, 2145, 2556
XspI CTAG 7 cut(s) 312, 755, 794, 803, 1169, 1187, 1700
ZrmI AGTACT 1 cut(s) 882
Zsp2I ATGCAT 1 cut(s) 1458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.