RchiOBHm_Chr5g0018151

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000110
Physical Location & Seq
Reverse (-)
12668377 .. 12671142
2766 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29837

Sequence Viewer

Length: 2766 bp
ATGGCCTTATCTGCTGCGGACCTCTTAATTGGAAAACTTGTGACAATTCTTGAGAACGAAGGAACAGCCATAGCAGGTGTTCGTGATCAAGTTGAAAAGATTAAGGAGGAGTTTCTAAGCATGAAAGCTTTCCTAGTGGATGCGGAAGCCAACAAAGCAAAAACTGAAGGAGAGAAATTGTGGGTTGCAAGAATCAGAGACTTGGCCCATGATGTTGAAGATACCATTGATGAATTCATGTATCAAACGTATGAGAAGCAAAGTGGGGGTCAAATTTCAAGGGGGCTAAACAAAACCATCTGTGGTCCGAGTAATCTTTGGTTCAGGCGTAAAACTGCAAAGAAGTTGCAGAAAATTACGGATATGATCAAAACCATTCCAGAGAGGAATCAAAGGTACGGTGTTGGTCTTTTAGGAGGAGCAATTGCATCTGAAGATATCCACAAATGTGTGCAGAACCAAGCGGACTCTTCTTTTTTCATTATGGAAGACGAACTGGTTGGGATTGAAGGCAAGAAGCAAATATTAATGGGATGGCTCATGGATGAAGAGCAACACCAAACTGTTATATCTGTCGTTGGGATGGGAGGTTCCGGAAAGACAACTCTAATTGCCAAGACCTTCTCCCACGAAAGGGTAAAGAGACATTTCGAATGTTCTGCGTGGATTACTGTTTCCCAAACTTATGCTATGGTCGACTTGTTTCGAAGCTTGATCATAGAACTCTACAAATCAAGGAAGGAAGATTTCCCTGCAAATTTGAATGCCATGAGCAGTAGAGAATTGATAGAGATACTGACGGACTACTTGGGGTCTAAAAAGTACCTAGTTGTTCTAGATGACGTGTGGGAGATAAGAGTTTGGAGAGAAATAAAGGTATCACTTCAAGATAGACAACGTGGAAGTCGAATCGTTCTCACCACTCGTAATGAAGAAGTAGCATCCAACTCTTTTGGAGTTGAAAGCTATGTTCACCATATTCAGCCCCTGCAAAAGAATGAGGCTTGGGAGCTCTTCAGCAAGAAAGCATTCTTAACTTATCAACACAGAACTTGTCCAACTGAGCTGAAGTCATTAGCTTTCCAACTTGTGGACAAGTGTGAAGGCCTTCCTCTGGCTATTGTGGCTTTAGGTGGTCTTATGTCTTCTAAGAAGTCGTTGGATCAATGGCAACAAGTCTGCAACAGCTTGAACTGGCATCTAGCTAATAATTCATCGCTAGACCCTGTGAAAAACATCTTGTTGCTGAGTTTCGATAATTTGCCATCCCAATTGAAACATTGCTTCCTCTATTGCTCTCTTTTCCCAGAAGATTTTGTAATTAAGAGAAAAAGGCTCATCAGATTGTGGATAGCTGAAGGATTCGTTGAACATGTGAAAGGGGTCACGCCCGAAGAAGTTGCAGATGGCTATCTTTGGGGACTCTGCTTCCACAACATGTTACAAGTTGTTGAGAGAAATGAAACAGGAAGGCCAAAAGAAGTTAAGATGCATGATCTTATGCGAGAGCTTGCTCTGTCGACATCTGAAAAAGAAAAGTTTGGTCATGTATATGACGGGAGAGAAGTAATTGAAGATATCTCAACTCGTCGCCTGTCAATTCACAGAACGAGTAATGGAGAAATTAAATCATGGCCAGGTATGTCAAAGATTCGTTCTCTTCTTGTCTTTGCAACTGACATGTCCTCATTGTCTTTCTCAAATGCACTGGTTTCTCAATTTAAGTTGTTGAGGACTCTAGACTTGGAGGATGTCCAAATTGATAAACTGCCGGATGCAGTTGTTTACTTGTTCAACTTGAGATACTTAAATTTGAAGGGTTCTTTAATAAAGGAACTTCCAGAGTCCATAGGACGTCTCCGCAACCTTCAATTTCTCAAGATGGACCGTACTAAGATAGAATTACTTCCACAAGGAATTGCAAAGTTGCTTAACTTGCGCTATCTAACTATGTATCGTCTTAATAAGTTAGAGTTATTTATGGGACAATTCAATATGATAAGTGGGACAAGAGCACCATCAAATATTTGTAAGTTGCAGCAATTACAATATTTAGGGTGTATTGAATCAGAGGGGAACATTGTTAGACTTGTTGGCAACATGACTCAACTTAAGAACATCGCCATATCGAATGTGAAAGAAAGAGATGAGATTGACCTTTGGGCCTCAATTAAGAAGATGACTCTGCTTCAAGGTCTGCAATTAATAGCAAGTAATGAAGAGGAAATTCTTCCGGTGAAAGCACAATGTTCACCTCTTCCGCACCTTCAACAGCTTTCTTTGATTGGCAGACTGCAAAATGTAATACCTTGGTTTTCTTCACTGCAGAGCCTCACAGCTTTGAGTCTGCATTGGTCTAGACTTGAAGAGGATTTACTTCCTTACATTGAAGTACTTCCCAATCTGACGCAACTTTTTCTTACTAATGCATATGTTGGGAAAGAGTTGTGTTTTGGCAGGGGTTTTGTAAAGCTTATGAGATTGCGCTTGGTAAATTTTGCGTTGTTGAAAAAGATAACTATAGAAAAAGGTGTGATGCCCAATCTCCAGTACTTATGGGTTGATAGCTGCAAGGATTTGAAGACAGTGCCACTGGGCCTTGAATATCTTTCTAATCTACAATCTTTGGGCTTGGCGGATGTTCCTATGGAAGTTATACAGCCCATACAGCGAGGAAGTGCTGATCACTTTAAGGTACAACACATCCCTGAGATCATACATGTTTTTGAACAATCTTCCAAGTGGGTCTCCGAAAACTTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

921

Amino Acids

105.14

Weight (kDa)

8.19

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 88 3.3e-22 Rx N-terminal domain
NB-ARC PF00931 172 - 344 3.4e-46 NB-ARC domain
WHD_DRP PF23559 434 - 505 1.4e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 550 - 876 5.4e-39 Leucine-rich repeat region
LRR_8 PF13855 577 - 633 2.2e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07040
fragaria_vesca FvH4_2g00960 FvH4_2g14930 FvH4_3g11470 FvH4_3g11471 FvH4_3g11481 FvH4_3g11501 FvH4_3g11502 FvH4_3g11503 FvH4_3g11503 FvH4_3g11503 FvH4_3g11504 FvH4_3g11505 FvH4_3g17400 FvH4_3g17401 FvH4_6g29690
malus_domestica MD00G1206000.v1.1 MD00G1207600.v1.1 MD00G1207700.v1.1 MD00G1207800.v1.1 MD05G1008300.v1.1 MD05G1008400.v1.1 MD05G1024900.v1.1 MD05G1025200.v1.1 MD06G1152000.v1.1 MD06G1152400.v1.1 MD06G1152500.v1.1 MD06G1152800.v1.1 MD06G1153100.v1.1 MD06G1153200.v1.1 MD06G1153300.v1.1 MD06G1153700.v1.1 MD06G1154100.v1.1 MD06G1154600.v1.1 MD06G1155300.v1.1 MD06G1155600.v1.1 MD06G1155800.v1.1 MD06G1156200.v1.1 MD06G1156300.v1.1 MD06G1156900.v1.1 MD06G1157000.v1.1 MD10G1003800.v1.1 MD15G1390600.v1.1
prunus_persica Prupe.6G146200_v2.0.a1 Prupe.6G146400_v2.0.a1 Prupe.6G194900_v2.0.a1 Prupe.6G199100_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199400_v2.0.a1 Prupe.6G199500_v2.0.a1 Prupe.6G199700_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G199800_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200200_v2.0.a1 Prupe.6G200400_v2.0.a1 Prupe.6G200600_v2.0.a1 Prupe.6G200900_v2.0.a1 Prupe.6G201100_v2.0.a1 Prupe.8G007600_v2.0.a1 Prupe.8G008500_v2.0.a1 Prupe.8G008600_v2.0.a1 Prupe.8G008700_v2.0.a1 Prupe.8G008900_v2.0.a1 Prupe.8G009000_v2.0.a1 Prupe.8G009300_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009400_v2.0.a1 Prupe.8G009500_v2.0.a1 Prupe.8G009600_v2.0.a1 Prupe.8G009700_v2.0.a1 Prupe.8G010000_v2.0.a1 Prupe.8G010200_v2.0.a1 Prupe.8G010600_v2.0.a1 Prupe.8G010700_v2.0.a1
pyrus_communis pycom05g00420 pycom05g01590 pycom05g01610 pycom05g01640 pycom06g14010 pycom06g14020 pycom06g14050 pycom10g00310 pycom15g34970
rosa_chinensis RchiOBHm_Chr1g0339061 RchiOBHm_Chr1g0348041 RchiOBHm_Chr2g0101551 RchiOBHm_Chr2g0101661 RchiOBHm_Chr2g0135281 RchiOBHm_Chr2g0135361 RchiOBHm_Chr2g0135381 RchiOBHm_Chr4g0410551 RchiOBHm_Chr5g0015461 RchiOBHm_Chr5g0018151 RchiOBHm_Chr5g0018401 RchiOBHm_Chr5g0018431 RchiOBHm_Chr5g0018441 RchiOBHm_Chr5g0018451 RchiOBHm_Chr5g0018461 RchiOBHm_Chr5g0052641 RchiOBHm_Chr5g0052661 RchiOBHm_Chr5g0052681 RchiOBHm_Chr5g0052691 RchiOBHm_Chr5g0052731 RchiOBHm_Chr6g0274421 RchiOBHm_Chr6g0277541 RchiOBHm_Chr6g0278191 RchiOBHm_Chr6g0278241
rosa_laevigata RLG00000013544 RLG00000014183 RLG00000017089 RLG00000019459 RLG00000019460 RLG00000032362 RLG00000032363 RLG00000032367
rosa_multiflora Rmu_co8364011.1_g000001 Rmu_co8390397.1_g000001 Rmu_sc0000694.1_g000010 Rmu_sc0000694.1_g000011 Rmu_sc0000958.1_g000006 Rmu_sc0001289.1_g000014 Rmu_sc0001382.1_g000019 Rmu_sc0002197.1_g000002 Rmu_sc0002197.1_g000036 Rmu_sc0002311.1_g000024 Rmu_sc0003835.1_g000020 Rmu_sc0003910.1_g000007 Rmu_sc0004464.1_g000013 Rmu_sc0006594.1_g000002 Rmu_sc0006594.1_g000011 Rmu_sc0010265.1_g000015 Rmu_sc0010265.1_g000019 Rmu_sc0013020.1_g000002 Rmu_sc0017459.1_g000009 Rmu_sc0034280.1_g000001 Rmu_ssc0000187.1_g000004 Rmu_ssc0000187.1_g000015 Rmu_ssc0000187.1_g000022 Rmu_ssc0000263.1_g000008
rosa_roxburghii Rroxscaffold_1G00028510 Rroxscaffold_1G00059270 Rroxscaffold_1G00059340 Rroxscaffold_1G00061030 Rroxscaffold_2G00109280 Rroxscaffold_2G00109320 Rroxscaffold_2G00141200 Rroxscaffold_4G00307310 Rroxscaffold_7G00191240 Rroxscaffold_7G00194240
rosa_rugosa Rorug01G0190800 Rorug02G0097500 Rorug02G0097600 Rorug02G0324500 Rorug02G0324600 Rorug03G0114500 Rorug05G0042600.1 Rorug05G0043300 Rorug05G0043400 Rorug05G0043600 Rorug05G0043600 Rorug05G0043800 Rorug05G0043900 Rorug05G0044100 Rorug05G0044300 Rorug05G0502000 Rorug05G0502100 Rorug05G0502200 Rorug05G0502300 Rorug05G0502400 Rorug06G0037200 Rorug06G0081600 Rorug06G0115000 Rorug06G0115100
rosa_samantha Rh1AG209400 Rh1BG174400 Rh1CG193800 Rh1DG140600 Rh2BG153300 Rh2BG381100 Rh2BG381200 Rh2BG381400 Rh2BG381500 Rh2BG381700 Rh2CG153800 Rh2CG360300 Rh2DG153600 Rh2DG397500 Rh2DG397800 Rh2DG398000 Rh4AG161500 Rh4BG162800 Rh4CG173900 Rh4CG175800 Rh5AG121200 Rh5AG121300 Rh5AG343200 Rh5AG343500 Rh5BG120100 Rh5BG132000 Rh5BG132100 Rh5BG134200 Rh5BG353100 Rh5BG353200 Rh5BG353400 Rh5BG353600 Rh5BG353700 Rh5BG353900 Rh5CG144300 Rh5CG145900 Rh5CG146200 Rh5CG146300 Rh5CG146400 Rh6AG011300 Rh6AG011400 Rh6AG197600 Rh6AG219900 Rh6BG010200 Rh6BG010300 Rh6BG201400 Rh6BG223800 Rh6DG222800 Rh6DG223000
rosa_wichuraiana Rw1G017450 Rw2G011530 Rw2G030640 Rw2G030650 Rw5G010460 Rw5G011650 Rw5G011860 Rw5G011900 Rw5G011920 Rw5G011970 Rw5G011980 Rw5G012000 Rw5G032280 Rw5G032330 Rw5G032450 Rw6G001330 Rw6G017220 Rw6G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 65
AatII GACGTC 1 cut(s) 1858
Acc36I ACCTGC 1 cut(s) 65
AccB7I CCANNNNNTGG 1 cut(s) 1090
AccI GTMKAC 2 cut(s) 696, 1520
AccIII TCCGGA 1 cut(s) 593
AciI CCGC 6 cut(s) 17, 143, 464, 1861, 2261, 2636
AclWI GGATC 1 cut(s) 1170
AcoI YGGCCR 1 cut(s) 1634
AcsI RAATTY 6 cut(s) 233, 273, 757, 1810, 2226, 2494
AcuI CTGAAG 5 cut(s) 186, 453, 1000, 1088, 1377
AcyI GRCGYC 1 cut(s) 1855
AfaI GTAC 6 cut(s) 398, 824, 1891, 2394, 2552, 2697
AfiI CCNNNNNNNGG 4 cut(s) 633, 634, 1090, 1114
AflII CTTAAG 1 cut(s) 2111
AflIII ACRYGT 5 cut(s) 843, 1372, 1437, 1680, 2719
AjiI CACGTC 1 cut(s) 844
AjnI CCWGG 1 cut(s) 1636
AleI CACNNNNGTG 1 cut(s) 447
AloI GAACNNNNNNTCC 2 cut(s) 574, 606
Alw21I GWGCWC 2 cut(s) 1014, 2017
Alw26I GTCTC 4 cut(s) 192, 637, 1862, 2752
AlwI GGATC 1 cut(s) 1170
AlwNI CAGNNNCTG 1 cut(s) 988
Aor13HI TCCGGA 1 cut(s) 593
AoxI GGCC 8 cut(s) 3, 204, 1105, 1472, 1634, 2163, 2596, 2760
ApeKI GCWGC 3 cut(s) 14, 2038, 2568
ApoI RAATTY 6 cut(s) 233, 273, 757, 1810, 2226, 2494
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
AseI ATTAAT 2 cut(s) 527, 2204
Asp700I GAANNNNTTC 6 cut(s) 128, 1028, 1107, 1905, 2229, 2394
AspLEI GCGC 2 cut(s) 1941, 2487
AspS9I GGNCC 6 cut(s) 19, 205, 305, 1885, 2163, 2596
AsuHPI GGTGA 4 cut(s) 910, 965, 2244, 2248
AsuII TTCGAA 2 cut(s) 651, 706
AvaII GGWCC 3 cut(s) 19, 305, 1885
BalI TGGCCA 1 cut(s) 1636
BanII GRGCYC 1 cut(s) 1014
BarI GAAGNNNNNNTAC 2 cut(s) 2358, 2390
BbsI GAAGAC 3 cut(s) 495, 1137, 2588
Bbv12I GWGCWC 2 cut(s) 1014, 2017
BbvI GCAGC 2 cut(s) 2050, 2555
BccI CCATC 7 cut(s) 305, 528, 577, 1273, 1400, 1876, 2026
BcgI CGANNNNNNTGC 4 cut(s) 641, 675, 1382, 1416
BciT130I CCWGG 1 cut(s) 1638
BclI TGATCA 4 cut(s) 85, 366, 714, 2683
BcoDI GTCTC 4 cut(s) 192, 637, 1862, 2752
BfaI CTAG 8 cut(s) 134, 827, 836, 1202, 1220, 1739, 2358, 2764
BfmI CTRYAG 2 cut(s) 2324, 2520
BfrI CTTAAG 1 cut(s) 2111
BfuAI ACCTGC 1 cut(s) 65
BisI GCNGC 3 cut(s) 15, 2039, 2569
BlsI GCNGC 3 cut(s) 16, 2040, 2570
BmcAI AGTACT 2 cut(s) 2394, 2552
Bme1390I CCNGG 1 cut(s) 1638
Bme18I GGWCC 3 cut(s) 19, 305, 1885
BmgBI CACGTC 1 cut(s) 844
BmgT120I GGNCC 6 cut(s) 19, 205, 305, 1885, 2163, 2596
BmiI GGNNCC 1 cut(s) 592
BmrFI CCNGG 1 cut(s) 1638
BmrI ACTGGG 1 cut(s) 2603
BmsI GCATC 7 cut(s) 130, 437, 950, 1207, 1479, 1765, 2526
BmuI ACTGGG 1 cut(s) 2603
BpiI GAAGAC 3 cut(s) 495, 1137, 2588
BpmI CTGGAG 1 cut(s) 2531
Bpu14I TTCGAA 2 cut(s) 651, 706
BpuEI CTTGAG 3 cut(s) 71, 1819, 1862
BsaBI GATNNNNATC 1 cut(s) 1410
BsaHI GRCGYC 1 cut(s) 1855
BsaI GGTCTC 1 cut(s) 2752
BsaJI CCNNGG 1 cut(s) 2309
BsaWI WCCGGW 2 cut(s) 593, 2233
Bsc4I CCNNNNNNNGG 4 cut(s) 633, 634, 1090, 1114
Bse1I ACTGG 5 cut(s) 501, 1199, 1713, 2548, 2598
Bse3DI GCAATG 1 cut(s) 1279
Bse8I GATNNNNATC 1 cut(s) 1410
BseAI TCCGGA 1 cut(s) 593
BseBI CCWGG 1 cut(s) 1638
BseDI CCNNGG 1 cut(s) 2309
BseJI GATNNNNATC 1 cut(s) 1410
BseLI CCNNNNNNNGG 4 cut(s) 633, 634, 1090, 1114
BseMI GCAATG 1 cut(s) 1279
BseMII CTCAG 3 cut(s) 1053, 1238, 2700
BseNI ACTGG 5 cut(s) 501, 1199, 1713, 2548, 2598
BseRI GAGGAG 2 cut(s) 122, 432
BseXI GCAGC 2 cut(s) 2050, 2555
BsgI GTGCAG 1 cut(s) 473
BshFI GGCC 8 cut(s) 5, 206, 1107, 1474, 1636, 2165, 2598, 2762
BsiHKAI GWGCWC 2 cut(s) 1014, 2017
BsiSI CCGG 3 cut(s) 594, 1772, 2234
BslFI GGGAC 3 cut(s) 1434, 1998, 2020
BslI CCNNNNNNNGG 4 cut(s) 633, 634, 1090, 1114
BsmAI GTCTC 4 cut(s) 192, 637, 1862, 2752
BsmBI CGTCTC 1 cut(s) 1862
BsmFI GGGAC 3 cut(s) 1434, 1998, 2020
BsmI GAATGC 2 cut(s) 769, 1028
BsnI GGCC 8 cut(s) 5, 206, 1107, 1474, 1636, 2165, 2598, 2762
Bso31I GGTCTC 1 cut(s) 2752
Bsp119I TTCGAA 2 cut(s) 651, 706
Bsp1286I GDGCHC 2 cut(s) 1014, 2017
Bsp13I TCCGGA 1 cut(s) 593
Bsp143I GATC 7 cut(s) 85, 366, 714, 1162, 1495, 2683, 2712
BspACI CCGC 6 cut(s) 17, 143, 464, 1861, 2261, 2636
BspANI GGCC 8 cut(s) 5, 206, 1107, 1474, 1636, 2165, 2598, 2762
BspCNI CTCAG 3 cut(s) 1054, 1239, 2701
BspEI TCCGGA 1 cut(s) 593
BspLI GGNNCC 1 cut(s) 592
BspMAI CTGCAG 1 cut(s) 2328
BspMI ACCTGC 1 cut(s) 65
BspPI GGATC 1 cut(s) 1170
BspQI GCTCTTC 2 cut(s) 543, 1019
BspT104I TTCGAA 2 cut(s) 651, 706
BspTI CTTAAG 1 cut(s) 2111
BspTNI GGTCTC 1 cut(s) 2752
BsrDI GCAATG 1 cut(s) 1279
BsrI ACTGG 5 cut(s) 501, 1199, 1713, 2548, 2598
BssECI CCNNGG 1 cut(s) 2309
BssMI GATC 7 cut(s) 85, 366, 714, 1162, 1495, 2683, 2712
BssNI GRCGYC 1 cut(s) 1855
BssT1I CCWWGG 1 cut(s) 2309
Bst2UI CCWGG 1 cut(s) 1638
Bst4CI ACNGT 5 cut(s) 401, 565, 673, 1889, 2587
Bst6I CTCTTC 7 cut(s) 475, 543, 1019, 1665, 2214, 2262, 2361
BstACI GRCGYC 1 cut(s) 1855
BstAFI CTTAAG 1 cut(s) 2111
BstBI TTCGAA 2 cut(s) 651, 706
BstC8I GCNNGC 1 cut(s) 1512
BstDEI CTNAG 6 cut(s) 116, 1062, 1149, 1247, 1893, 2709
BstHHI GCGC 2 cut(s) 1941, 2487
BstKTI GATC 7 cut(s) 88, 369, 717, 1165, 1498, 2686, 2715
BstMAI GTCTC 4 cut(s) 192, 637, 1862, 2752
BstMBI GATC 7 cut(s) 85, 366, 714, 1162, 1495, 2683, 2712
BstMWI GCNNNNNNNGC 5 cut(s) 11, 155, 1124, 1936, 2668
BstNI CCWGG 1 cut(s) 1638
BstNSI RCATGY 4 cut(s) 1376, 1441, 1684, 2723
BstSCI CCNGG 1 cut(s) 1636
BstSFI CTRYAG 2 cut(s) 2324, 2520
BstV1I GCAGC 2 cut(s) 2050, 2555
BstV2I GAAGAC 3 cut(s) 495, 1137, 2588
BsuRI GGCC 8 cut(s) 5, 206, 1107, 1474, 1636, 2165, 2598, 2762
BtgZI GCGATG 2 cut(s) 1200, 2104
BtrI CACGTC 1 cut(s) 844
BtsI GCAGTG 1 cut(s) 2321
BtsIMutI CAGTG 4 cut(s) 1706, 2321, 2591, 2592
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 1 cut(s) 1512
CaiI CAGNNNCTG 1 cut(s) 988
CfoI GCGC 2 cut(s) 1941, 2487
Cfr13I GGNCC 6 cut(s) 19, 205, 305, 1885, 2163, 2596
CseI GACGC 1 cut(s) 2416
Csp6I GTAC 6 cut(s) 397, 823, 1890, 2393, 2551, 2696
CviQI GTAC 6 cut(s) 397, 823, 1890, 2393, 2551, 2696
DdeI CTNAG 6 cut(s) 116, 1062, 1149, 1247, 1893, 2709
DpnI GATC 7 cut(s) 87, 368, 716, 1164, 1497, 2685, 2714
DpnII GATC 7 cut(s) 85, 366, 714, 1162, 1495, 2683, 2712
EaeI YGGCCR 1 cut(s) 1634
Eam1104I CTCTTC 7 cut(s) 475, 543, 1019, 1665, 2214, 2262, 2361
EarI CTCTTC 7 cut(s) 475, 543, 1019, 1665, 2214, 2262, 2361
EciI GGCGGA 1 cut(s) 2651
Ecl136II GAGCTC 1 cut(s) 1012
Eco130I CCWWGG 1 cut(s) 2309
Eco147I AGGCCT 1 cut(s) 1107
Eco24I GRGCYC 1 cut(s) 1014
Eco31I GGTCTC 1 cut(s) 2752
Eco32I GATATC 2 cut(s) 439, 1579
Eco47I GGWCC 3 cut(s) 19, 305, 1885
Eco53kI GAGCTC 1 cut(s) 1012
Eco57I CTGAAG 5 cut(s) 186, 453, 1000, 1088, 1377
EcoICRI GAGCTC 1 cut(s) 1012
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 1636
EcoRV GATATC 2 cut(s) 439, 1579
EcoT14I CCWWGG 1 cut(s) 2309
EcoT22I ATGCAT 2 cut(s) 1494, 2431
EcoT38I GRGCYC 1 cut(s) 1014
ErhI CCWWGG 1 cut(s) 2309
Esp3I CGTCTC 1 cut(s) 1862
FaqI GGGAC 3 cut(s) 1434, 1998, 2020
FauNDI CATATG 1 cut(s) 2431
FbaI TGATCA 4 cut(s) 85, 366, 714, 2683
FblI GTMKAC 2 cut(s) 696, 1520
Fnu4HI GCNGC 3 cut(s) 15, 2039, 2569
FriOI GRGCYC 1 cut(s) 1014
Fsp4HI GCNGC 3 cut(s) 15, 2039, 2569
FspBI CTAG 8 cut(s) 134, 827, 836, 1202, 1220, 1739, 2358, 2764
GlaI GCGC 2 cut(s) 1940, 2486
GluI GCNGC 3 cut(s) 15, 2039, 2569
GsuI CTGGAG 1 cut(s) 2531
HaeIII GGCC 8 cut(s) 5, 206, 1107, 1474, 1636, 2165, 2598, 2762
HapII CCGG 3 cut(s) 594, 1772, 2234
HgaI GACGC 1 cut(s) 2416
HhaI GCGC 2 cut(s) 1941, 2487
Hin1I GRCGYC 1 cut(s) 1855
Hin6I GCGC 2 cut(s) 1939, 2485
HinP1I GCGC 2 cut(s) 1939, 2485
HincII GTYRAC 2 cut(s) 697, 1521
HindII GTYRAC 2 cut(s) 697, 1521
HindIII AAGCTT 3 cut(s) 126, 709, 2471
HpaII CCGG 3 cut(s) 594, 1772, 2234
HphI GGTGA 4 cut(s) 910, 965, 2244, 2248
Hpy166II GTNNAC 6 cut(s) 697, 973, 1093, 1521, 1786, 2252
Hpy188I TCNGA 8 cut(s) 197, 309, 433, 1343, 1528, 2071, 2406, 2752
Hpy8I GTNNAC 6 cut(s) 697, 973, 1093, 1521, 1786, 2252
Hpy99I CGWCG 1 cut(s) 1593
HpyCH4III ACNGT 5 cut(s) 401, 565, 673, 1889, 2587
HpyCH4IV ACGT 4 cut(s) 248, 843, 898, 1855
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 155, 1124, 1936, 2668
HpyF3I CTNAG 6 cut(s) 116, 1062, 1149, 1247, 1893, 2709
HpySE526I ACGT 4 cut(s) 248, 843, 898, 1855
Hsp92I GRCGYC 1 cut(s) 1855
HspAI GCGC 2 cut(s) 1939, 2485
Kpn2I TCCGGA 1 cut(s) 593
Ksp22I TGATCA 4 cut(s) 85, 366, 714, 2683
Kzo9I GATC 7 cut(s) 85, 366, 714, 1162, 1495, 2683, 2712
LguI GCTCTTC 2 cut(s) 543, 1019
LmnI GCTCC 2 cut(s) 419, 1009
Lsp1109I GCAGC 2 cut(s) 2050, 2555
LweI GCATC 7 cut(s) 130, 437, 950, 1207, 1479, 1765, 2526
MaeI CTAG 8 cut(s) 134, 827, 836, 1202, 1220, 1739, 2358, 2764
MaeII ACGT 4 cut(s) 248, 843, 898, 1855
MaeIII GTNAC 3 cut(s) 40, 1384, 1440
MalI GATC 7 cut(s) 87, 368, 716, 1164, 1497, 2685, 2714
MboI GATC 7 cut(s) 85, 366, 714, 1162, 1495, 2683, 2712
MfeI CAATTG 2 cut(s) 423, 1271
MhlI GDGCHC 2 cut(s) 1014, 2017
MlsI TGGCCA 1 cut(s) 1636
MluNI TGGCCA 1 cut(s) 1636
MlyI GAGTC 7 cut(s) 461, 1416, 1729, 1853, 2098, 2176, 2353
MmeI TCCRAC 4 cut(s) 969, 1082, 1108, 1140
Mox20I TGGCCA 1 cut(s) 1636
Mph1103I ATGCAT 2 cut(s) 1494, 2431
MroI TCCGGA 1 cut(s) 593
MroXI GAANNNNTTC 6 cut(s) 128, 1028, 1107, 1905, 2229, 2394
MscI TGGCCA 1 cut(s) 1636
MslI CAYNNNNRTG 2 cut(s) 447, 1551
Msp20I TGGCCA 1 cut(s) 1636
MspCI CTTAAG 1 cut(s) 2111
MspI CCGG 3 cut(s) 594, 1772, 2234
MspR9I CCNGG 1 cut(s) 1638
MunI CAATTG 2 cut(s) 423, 1271
Mva1269I GAATGC 2 cut(s) 769, 1028
MvaI CCWGG 1 cut(s) 1638
MwoI GCNNNNNNNGC 5 cut(s) 11, 155, 1124, 1936, 2668
NdeI CATATG 1 cut(s) 2431
NdeII GATC 7 cut(s) 85, 366, 714, 1162, 1495, 2683, 2712
NlaIV GGNNCC 1 cut(s) 592
NmuCI GTSAC 2 cut(s) 40, 1384
NsiI ATGCAT 2 cut(s) 1494, 2431
NspI RCATGY 4 cut(s) 1376, 1441, 1684, 2723
NspV TTCGAA 2 cut(s) 651, 706
OliI CACNNNNGTG 1 cut(s) 447
PaqCI CACCTGC 1 cut(s) 65
PceI AGGCCT 1 cut(s) 1107
PciI ACATGT 4 cut(s) 1372, 1437, 1680, 2719
PciSI GCTCTTC 2 cut(s) 543, 1019
PcsI WCGNNNNNNNCGW 1 cut(s) 904
PctI GAATGC 2 cut(s) 769, 1028
PdmI GAANNNNTTC 6 cut(s) 128, 1028, 1107, 1905, 2229, 2394
PfeI GAWTC 6 cut(s) 192, 388, 909, 1362, 1651, 2066
PflMI CCANNNNNTGG 1 cut(s) 1090
PkrI GCNGC 3 cut(s) 16, 2040, 2570
PleI GAGTC 7 cut(s) 461, 1416, 1729, 1852, 2098, 2176, 2352
PpsI GAGTC 7 cut(s) 461, 1416, 1729, 1852, 2098, 2176, 2352
PscI ACATGT 4 cut(s) 1372, 1437, 1680, 2719
PshBI ATTAAT 2 cut(s) 527, 2204
Psp124BI GAGCTC 1 cut(s) 1014
Psp6I CCWGG 1 cut(s) 1636
PspGI CCWGG 1 cut(s) 1636
PspN4I GGNNCC 1 cut(s) 592
PspPI GGNCC 6 cut(s) 19, 205, 305, 1885, 2163, 2596
PstI CTGCAG 1 cut(s) 2328
PstNI CAGNNNCTG 1 cut(s) 988
RsaI GTAC 6 cut(s) 398, 824, 1891, 2394, 2552, 2697
RsaNI GTAC 6 cut(s) 397, 823, 1890, 2393, 2551, 2696
RseI CAYNNNNRTG 2 cut(s) 447, 1551
SacI GAGCTC 1 cut(s) 1014
SalI GTCGAC 2 cut(s) 695, 1519
SapI GCTCTTC 2 cut(s) 543, 1019
SatI GCNGC 3 cut(s) 15, 2039, 2569
Sau3AI GATC 7 cut(s) 85, 366, 714, 1162, 1495, 2683, 2712
Sau96I GGNCC 6 cut(s) 19, 205, 305, 1885, 2163, 2596
ScaI AGTACT 2 cut(s) 2394, 2552
SchI GAGTC 7 cut(s) 461, 1416, 1729, 1853, 2098, 2176, 2353
ScrFI CCNGG 1 cut(s) 1638
SduI GDGCHC 2 cut(s) 1014, 2017
SfaNI GCATC 7 cut(s) 130, 437, 950, 1207, 1479, 1765, 2526
SfcI CTRYAG 2 cut(s) 2324, 2520
SfuI TTCGAA 2 cut(s) 651, 706
SinI GGWCC 3 cut(s) 19, 305, 1885
SmiMI CAYNNNNRTG 2 cut(s) 447, 1551
SmlI CTYRAG 4 cut(s) 50, 1798, 1877, 2111
SmoI CTYRAG 4 cut(s) 50, 1798, 1877, 2111
SseBI AGGCCT 1 cut(s) 1107
SsiI CCGC 6 cut(s) 17, 143, 464, 1861, 2261, 2636
SspI AATATT 3 cut(s) 525, 2026, 2051
SspMI CTAG 8 cut(s) 134, 827, 836, 1202, 1220, 1739, 2358, 2764
SstI GAGCTC 1 cut(s) 1014
StuI AGGCCT 1 cut(s) 1107
StyD4I CCNGG 1 cut(s) 1636
StyI CCWWGG 1 cut(s) 2309
TaaI ACNGT 5 cut(s) 401, 565, 673, 1889, 2587
TaiI ACGT 4 cut(s) 251, 846, 901, 1858
TaqI TCGA 7 cut(s) 651, 696, 706, 907, 1254, 1520, 2129
TatI WGTACW 2 cut(s) 2392, 2550
TfiI GAWTC 6 cut(s) 192, 388, 909, 1362, 1651, 2066
TscAI CASTG 4 cut(s) 1713, 2328, 2592, 2598
TseFI GTSAC 2 cut(s) 40, 1384
TseI GCWGC 3 cut(s) 14, 2038, 2568
Tsp45I GTSAC 2 cut(s) 40, 1384
TspDTI ATGAA 9 cut(s) 137, 226, 246, 469, 561, 945, 1203, 1476, 2232
TspGWI ACGGA 2 cut(s) 374, 815
TspRI CASTG 4 cut(s) 1713, 2328, 2592, 2598
Van91I CCANNNNNTGG 1 cut(s) 1090
Vha464I CTTAAG 1 cut(s) 2111
VpaK11BI GGWCC 3 cut(s) 19, 305, 1885
VspI ATTAAT 2 cut(s) 527, 2204
XapI RAATTY 6 cut(s) 233, 273, 757, 1810, 2226, 2494
XbaI TCTAGA 3 cut(s) 835, 1738, 2357
XceI RCATGY 4 cut(s) 1376, 1441, 1684, 2723
XmiI GTMKAC 2 cut(s) 696, 1520
XmnI GAANNNNTTC 6 cut(s) 128, 1028, 1107, 1905, 2229, 2394
XspI CTAG 8 cut(s) 134, 827, 836, 1202, 1220, 1739, 2358, 2764
ZraI GACGTC 1 cut(s) 1856
ZrmI AGTACT 2 cut(s) 2394, 2552
Zsp2I ATGCAT 2 cut(s) 1494, 2431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.