Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 11 |
| AccI | GTMKAC | 2 cut(s) | 54, 165 |
| AccII | CGCG | 1 cut(s) | 227 |
| AciI | CCGC | 8 cut(s) | 6, 9, 182, 225, 518, 584, 644, 677 |
| AclWI | GGATC | 1 cut(s) | 277 |
| AcoI | YGGCCR | 2 cut(s) | 3, 222 |
| AcsI | RAATTY | 1 cut(s) | 717 |
| AcuI | CTGAAG | 1 cut(s) | 374 |
| AcyI | GRCGYC | 3 cut(s) | 41, 207, 264 |
| AfaI | GTAC | 2 cut(s) | 560, 786 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 26, 577 |
| AflII | CTTAAG | 1 cut(s) | 488 |
| AgsI | TTSAA | 3 cut(s) | 139, 385, 470 |
| AjnI | CCWGG | 2 cut(s) | 248, 777 |
| AluBI | AGCT | 4 cut(s) | 143, 240, 346, 358 |
| AluI | AGCT | 4 cut(s) | 143, 240, 346, 358 |
| Alw21I | GWGCWC | 2 cut(s) | 348, 428 |
| Alw26I | GTCTC | 3 cut(s) | 77, 239, 381 |
| AlwI | GGATC | 1 cut(s) | 277 |
| Ama87I | CYCGRG | 2 cut(s) | 235, 339 |
| AoxI | GGCC | 3 cut(s) | 3, 222, 336 |
| ApeKI | GCWGC | 1 cut(s) | 327 |
| ApoI | RAATTY | 1 cut(s) | 717 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 392, 424 |
| AspLEI | GCGC | 1 cut(s) | 259 |
| AspS9I | GGNCC | 2 cut(s) | 337, 408 |
| AsuC2I | CCSGG | 1 cut(s) | 572 |
| AsuHPI | GGTGA | 2 cut(s) | 9, 97 |
| AsuII | TTCGAA | 1 cut(s) | 704 |
| AvaI | CYCGRG | 2 cut(s) | 235, 339 |
| AvaII | GGWCC | 1 cut(s) | 408 |
| BanII | GRGCYC | 1 cut(s) | 348 |
| Bbv12I | GWGCWC | 2 cut(s) | 348, 428 |
| BbvI | GCAGC | 1 cut(s) | 314 |
| BccI | CCATC | 6 cut(s) | 71, 116, 182, 247, 268, 605 |
| BceAI | ACGGC | 3 cut(s) | 184, 237, 351 |
| BciT130I | CCWGG | 2 cut(s) | 250, 779 |
| BcnI | CCSGG | 1 cut(s) | 572 |
| BcoDI | GTCTC | 3 cut(s) | 77, 239, 381 |
| BfrI | CTTAAG | 1 cut(s) | 488 |
| BisI | GCNGC | 7 cut(s) | 6, 9, 182, 225, 328, 585, 645 |
| BlsI | GCNGC | 7 cut(s) | 7, 10, 183, 226, 329, 586, 646 |
| Bme1390I | CCNGG | 3 cut(s) | 250, 572, 779 |
| Bme18I | GGWCC | 1 cut(s) | 408 |
| BmeT110I | CYCGRG | 2 cut(s) | 235, 339 |
| BmgT120I | GGNCC | 2 cut(s) | 337, 408 |
| BmiI | GGNNCC | 1 cut(s) | 172 |
| BmrFI | CCNGG | 3 cut(s) | 250, 572, 779 |
| BmsI | GCATC | 2 cut(s) | 505, 679 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 356, 388 |
| BpmI | CTGGAG | 3 cut(s) | 52, 380, 653 |
| Bpu14I | TTCGAA | 1 cut(s) | 704 |
| BpuMI | CCSGG | 1 cut(s) | 572 |
| BsaHI | GRCGYC | 3 cut(s) | 41, 207, 264 |
| BsaI | GGTCTC | 1 cut(s) | 239 |
| BsaJI | CCNNGG | 3 cut(s) | 154, 429, 778 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 26, 577 |
| Bse118I | RCCGGY | 1 cut(s) | 266 |
| Bse1I | ACTGG | 1 cut(s) | 69 |
| Bse3DI | GCAATG | 1 cut(s) | 730 |
| BseBI | CCWGG | 2 cut(s) | 250, 779 |
| BseDI | CCNNGG | 3 cut(s) | 154, 429, 778 |
| BseGI | GGATG | 2 cut(s) | 258, 616 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 26, 577 |
| BseMI | GCAATG | 1 cut(s) | 730 |
| BseMII | CTCAG | 3 cut(s) | 546, 585, 707 |
| BseNI | ACTGG | 1 cut(s) | 69 |
| BseRI | GAGGAG | 4 cut(s) | 143, 377, 650, 653 |
| BseX3I | CGGCCG | 1 cut(s) | 222 |
| BseXI | GCAGC | 1 cut(s) | 314 |
| BsgI | GTGCAG | 1 cut(s) | 479 |
| Bsh1236I | CGCG | 1 cut(s) | 227 |
| Bsh1285I | CGRYCG | 1 cut(s) | 225 |
| BshFI | GGCC | 3 cut(s) | 5, 224, 338 |
| BsiEI | CGRYCG | 1 cut(s) | 225 |
| BsiHKAI | GWGCWC | 2 cut(s) | 348, 428 |
| BsiHKCI | CYCGRG | 2 cut(s) | 235, 339 |
| BsiSI | CCGG | 6 cut(s) | 27, 44, 185, 210, 267, 571 |
| BslFI | GGGAC | 1 cut(s) | 494 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 26, 577 |
| BsmAI | GTCTC | 3 cut(s) | 77, 239, 381 |
| BsmBI | CGTCTC | 1 cut(s) | 381 |
| BsmFI | GGGAC | 1 cut(s) | 494 |
| BsnI | GGCC | 3 cut(s) | 5, 224, 338 |
| Bso31I | GGTCTC | 1 cut(s) | 239 |
| BsoBI | CYCGRG | 2 cut(s) | 235, 339 |
| Bsp119I | TTCGAA | 1 cut(s) | 704 |
| Bsp1286I | GDGCHC | 2 cut(s) | 348, 428 |
| Bsp143I | GATC | 2 cut(s) | 228, 282 |
| BspACI | CCGC | 8 cut(s) | 6, 9, 182, 225, 518, 584, 644, 677 |
| BspANI | GGCC | 3 cut(s) | 5, 224, 338 |
| BspCNI | CTCAG | 3 cut(s) | 547, 586, 706 |
| BspFNI | CGCG | 1 cut(s) | 227 |
| BspLI | GGNNCC | 1 cut(s) | 172 |
| BspPI | GGATC | 1 cut(s) | 277 |
| BspT104I | TTCGAA | 1 cut(s) | 704 |
| BspTI | CTTAAG | 1 cut(s) | 488 |
| BspTNI | GGTCTC | 1 cut(s) | 239 |
| BsrBI | CCGCTC | 1 cut(s) | 11 |
| BsrDI | GCAATG | 1 cut(s) | 730 |
| BsrFI | RCCGGY | 1 cut(s) | 266 |
| BsrI | ACTGG | 1 cut(s) | 69 |
| BssAI | RCCGGY | 1 cut(s) | 266 |
| BssECI | CCNNGG | 3 cut(s) | 154, 429, 778 |
| BssMI | GATC | 2 cut(s) | 228, 282 |
| BssNI | GRCGYC | 3 cut(s) | 41, 207, 264 |
| BssT1I | CCWWGG | 1 cut(s) | 429 |
| Bst2UI | CCWGG | 2 cut(s) | 250, 779 |
| BstACI | GRCGYC | 3 cut(s) | 41, 207, 264 |
| BstAFI | CTTAAG | 1 cut(s) | 488 |
| BstBI | TTCGAA | 1 cut(s) | 704 |
| BstC8I | GCNNGC | 1 cut(s) | 268 |
| BstDEI | CTNAG | 4 cut(s) | 36, 555, 594, 693 |
| BstF5I | GGATG | 2 cut(s) | 258, 616 |
| BstFNI | CGCG | 1 cut(s) | 227 |
| BstHHI | GCGC | 1 cut(s) | 259 |
| BstKTI | GATC | 2 cut(s) | 231, 285 |
| BstMAI | GTCTC | 3 cut(s) | 77, 239, 381 |
| BstMBI | GATC | 2 cut(s) | 228, 282 |
| BstMCI | CGRYCG | 1 cut(s) | 225 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 263, 581, 653 |
| BstNI | CCWGG | 2 cut(s) | 250, 779 |
| BstSCI | CCNGG | 3 cut(s) | 248, 570, 777 |
| BstUI | CGCG | 1 cut(s) | 227 |
| BstV1I | GCAGC | 1 cut(s) | 314 |
| BstZI | CGGCCG | 1 cut(s) | 222 |
| BsuRI | GGCC | 3 cut(s) | 5, 224, 338 |
| BtsCI | GGATG | 2 cut(s) | 258, 616 |
| BtsIMutI | CAGTG | 2 cut(s) | 701, 716 |
| Cac8I | GCNNGC | 1 cut(s) | 268 |
| CfoI | GCGC | 1 cut(s) | 259 |
| Cfr10I | RCCGGY | 1 cut(s) | 266 |
| Cfr13I | GGNCC | 2 cut(s) | 337, 408 |
| CseI | GACGC | 3 cut(s) | 49, 215, 272 |
| Csp6I | GTAC | 2 cut(s) | 559, 785 |
| CviAII | CATG | 2 cut(s) | 449, 533 |
| CviQI | GTAC | 2 cut(s) | 559, 785 |
| DdeI | CTNAG | 4 cut(s) | 36, 555, 594, 693 |
| DpnI | GATC | 2 cut(s) | 230, 284 |
| DpnII | GATC | 2 cut(s) | 228, 282 |
| EaeI | YGGCCR | 2 cut(s) | 3, 222 |
| EagI | CGGCCG | 1 cut(s) | 222 |
| Ecl136II | GAGCTC | 1 cut(s) | 346 |
| EclXI | CGGCCG | 1 cut(s) | 222 |
| Eco130I | CCWWGG | 1 cut(s) | 429 |
| Eco24I | GRGCYC | 1 cut(s) | 348 |
| Eco31I | GGTCTC | 1 cut(s) | 239 |
| Eco47I | GGWCC | 1 cut(s) | 408 |
| Eco52I | CGGCCG | 1 cut(s) | 222 |
| Eco53kI | GAGCTC | 1 cut(s) | 346 |
| Eco57I | CTGAAG | 1 cut(s) | 374 |
| Eco88I | CYCGRG | 2 cut(s) | 235, 339 |
| EcoICRI | GAGCTC | 1 cut(s) | 346 |
| EcoRII | CCWGG | 2 cut(s) | 248, 777 |
| EcoT14I | CCWWGG | 1 cut(s) | 429 |
| EcoT38I | GRGCYC | 1 cut(s) | 348 |
| ErhI | CCWWGG | 1 cut(s) | 429 |
| Esp3I | CGTCTC | 1 cut(s) | 381 |
| FaeI | CATG | 2 cut(s) | 452, 536 |
| FaiI | YATR | 8 cut(s) | 77, 134, 450, 456, 534, 621, 738, 775 |
| FaqI | GGGAC | 1 cut(s) | 494 |
| FatI | CATG | 2 cut(s) | 448, 532 |
| FblI | GTMKAC | 2 cut(s) | 54, 165 |
| Fnu4HI | GCNGC | 7 cut(s) | 6, 9, 182, 225, 328, 585, 645 |
| FokI | GGATG | 2 cut(s) | 265, 623 |
| FriOI | GRGCYC | 1 cut(s) | 348 |
| Fsp4HI | GCNGC | 7 cut(s) | 6, 9, 182, 225, 328, 585, 645 |
| GlaI | GCGC | 1 cut(s) | 258 |
| GluI | GCNGC | 7 cut(s) | 6, 9, 182, 225, 328, 585, 645 |
| GsuI | CTGGAG | 3 cut(s) | 52, 380, 653 |
| HaeIII | GGCC | 3 cut(s) | 5, 224, 338 |
| HapII | CCGG | 6 cut(s) | 27, 44, 185, 210, 267, 571 |
| HgaI | GACGC | 3 cut(s) | 49, 215, 272 |
| HhaI | GCGC | 1 cut(s) | 259 |
| Hin1I | GRCGYC | 3 cut(s) | 41, 207, 264 |
| Hin1II | CATG | 2 cut(s) | 452, 536 |
| Hin6I | GCGC | 1 cut(s) | 257 |
| HinP1I | GCGC | 1 cut(s) | 257 |
| HincII | GTYRAC | 1 cut(s) | 166 |
| HindII | GTYRAC | 1 cut(s) | 166 |
| HinfI | GANTC | 5 cut(s) | 87, 440, 661, 706, 790 |
| HpaII | CCGG | 6 cut(s) | 27, 44, 185, 210, 267, 571 |
| HphI | GGTGA | 2 cut(s) | 9, 97 |
| Hpy166II | GTNNAC | 2 cut(s) | 55, 166 |
| Hpy188I | TCNGA | 2 cut(s) | 123, 412 |
| Hpy188III | TCNNGA | 2 cut(s) | 112, 397 |
| Hpy8I | GTNNAC | 2 cut(s) | 55, 166 |
| Hpy99I | CGWCG | 5 cut(s) | 167, 170, 209, 223, 266 |
| HpyCH4IV | ACGT | 2 cut(s) | 48, 162 |
| HpyCH4V | TGCA | 2 cut(s) | 460, 723 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 263, 581, 653 |
| HpyF3I | CTNAG | 4 cut(s) | 36, 555, 594, 693 |
| HpySE526I | ACGT | 2 cut(s) | 48, 162 |
| Hsp92I | GRCGYC | 3 cut(s) | 41, 207, 264 |
| Hsp92II | CATG | 2 cut(s) | 452, 536 |
| HspAI | GCGC | 1 cut(s) | 257 |
| KroI | GCCGGC | 1 cut(s) | 266 |
| KroNI | GCCGGC | 1 cut(s) | 268 |
| Kzo9I | GATC | 2 cut(s) | 228, 282 |
| LmnI | GCTCC | 3 cut(s) | 16, 176, 423 |
| Lsp1109I | GCAGC | 1 cut(s) | 314 |
| LweI | GCATC | 2 cut(s) | 505, 679 |
| MaeII | ACGT | 2 cut(s) | 48, 162 |
| MaeIII | GTNAC | 1 cut(s) | 15 |
| MalI | GATC | 2 cut(s) | 230, 284 |
| MbiI | CCGCTC | 1 cut(s) | 11 |
| MboI | GATC | 2 cut(s) | 228, 282 |
| MboII | GAAGA | 1 cut(s) | 482 |
| MhlI | GDGCHC | 2 cut(s) | 348, 428 |
| MluCI | AATT | 4 cut(s) | 367, 465, 474, 717 |
| MlyI | GAGTC | 2 cut(s) | 81, 434 |
| MmeI | TCCRAC | 3 cut(s) | 123, 324, 588 |
| MreI | CGCCGGCG | 1 cut(s) | 266 |
| MroNI | GCCGGC | 1 cut(s) | 266 |
| MseI | TTAA | 1 cut(s) | 489 |
| MslI | CAYNNNNRTG | 1 cut(s) | 453 |
| MspCI | CTTAAG | 1 cut(s) | 488 |
| MspI | CCGG | 6 cut(s) | 27, 44, 185, 210, 267, 571 |
| MspR9I | CCNGG | 3 cut(s) | 250, 572, 779 |
| MvaI | CCWGG | 2 cut(s) | 250, 779 |
| MvnI | CGCG | 1 cut(s) | 227 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 263, 581, 653 |
| NaeI | GCCGGC | 1 cut(s) | 268 |
| NciI | CCSGG | 1 cut(s) | 572 |
| NdeII | GATC | 2 cut(s) | 228, 282 |
| NgoMIV | GCCGGC | 1 cut(s) | 266 |
| NlaIII | CATG | 2 cut(s) | 452, 536 |
| NlaIV | GGNNCC | 1 cut(s) | 172 |
| NmeAIII | GCCGAG | 2 cut(s) | 135, 284 |
| NmuCI | GTSAC | 1 cut(s) | 15 |
| NspV | TTCGAA | 1 cut(s) | 704 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 162, 267, 584 |
| PdiI | GCCGGC | 1 cut(s) | 268 |
| PfeI | GAWTC | 3 cut(s) | 661, 706, 790 |
| PflFI | GACNNNGTC | 1 cut(s) | 50 |
| PkrI | GCNGC | 7 cut(s) | 7, 10, 183, 226, 329, 586, 646 |
| PleI | GAGTC | 2 cut(s) | 81, 434 |
| PpsI | GAGTC | 2 cut(s) | 81, 434 |
| Psp124BI | GAGCTC | 1 cut(s) | 348 |
| Psp6I | CCWGG | 2 cut(s) | 248, 777 |
| PspGI | CCWGG | 2 cut(s) | 248, 777 |
| PspN4I | GGNNCC | 1 cut(s) | 172 |
| PspPI | GGNCC | 2 cut(s) | 337, 408 |
| PsyI | GACNNNGTC | 1 cut(s) | 50 |
| RsaI | GTAC | 2 cut(s) | 560, 786 |
| RsaNI | GTAC | 2 cut(s) | 559, 785 |
| RseI | CAYNNNNRTG | 1 cut(s) | 453 |
| SacI | GAGCTC | 1 cut(s) | 348 |
| SalI | GTCGAC | 1 cut(s) | 164 |
| SaqAI | TTAA | 1 cut(s) | 489 |
| SatI | GCNGC | 7 cut(s) | 6, 9, 182, 225, 328, 585, 645 |
| Sau3AI | GATC | 2 cut(s) | 228, 282 |
| Sau96I | GGNCC | 2 cut(s) | 337, 408 |
| SchI | GAGTC | 2 cut(s) | 81, 434 |
| ScrFI | CCNGG | 3 cut(s) | 250, 572, 779 |
| SduI | GDGCHC | 2 cut(s) | 348, 428 |
| SetI | ASST | 9 cut(s) | 26, 51, 145, 165, 242, 251, 348, 360, 410 |
| SfaNI | GCATC | 2 cut(s) | 505, 679 |
| SfuI | TTCGAA | 1 cut(s) | 704 |
| SgrAI | CRCCGGYG | 1 cut(s) | 266 |
| SgrDI | CGTCGACG | 1 cut(s) | 164 |
| SinI | GGWCC | 1 cut(s) | 408 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 453 |
| SmlI | CTYRAG | 1 cut(s) | 488 |
| SmoI | CTYRAG | 1 cut(s) | 488 |
| Sse9I | AATT | 4 cut(s) | 367, 465, 474, 717 |
| SsiI | CCGC | 8 cut(s) | 6, 9, 182, 225, 518, 584, 644, 677 |
| SstI | GAGCTC | 1 cut(s) | 348 |
| StyD4I | CCNGG | 3 cut(s) | 248, 570, 777 |
| StyI | CCWWGG | 1 cut(s) | 429 |
| TaiI | ACGT | 2 cut(s) | 51, 165 |
| TaqI | TCGA | 3 cut(s) | 90, 165, 704 |
| TasI | AATT | 4 cut(s) | 367, 465, 474, 717 |
| TatI | WGTACW | 1 cut(s) | 558 |
| TauI | GCSGC | 6 cut(s) | 8, 11, 184, 227, 587, 647 |
| TfiI | GAWTC | 3 cut(s) | 661, 706, 790 |
| Tru1I | TTAA | 1 cut(s) | 489 |
| Tru9I | TTAA | 1 cut(s) | 489 |
| TscAI | CASTG | 2 cut(s) | 701, 716 |
| TseFI | GTSAC | 1 cut(s) | 15 |
| TseI | GCWGC | 1 cut(s) | 327 |
| Tsp45I | GTSAC | 1 cut(s) | 15 |
| TspDTI | ATGAA | 2 cut(s) | 437, 521 |
| TspRI | CASTG | 2 cut(s) | 701, 716 |
| Tth111I | GACNNNGTC | 1 cut(s) | 50 |
| Vha464I | CTTAAG | 1 cut(s) | 488 |
| VpaK11BI | GGWCC | 1 cut(s) | 408 |
| XapI | RAATTY | 1 cut(s) | 717 |
| XmiI | GTMKAC | 2 cut(s) | 54, 165 |