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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 239 |
| AccBSI | CCGCTC | 1 cut(s) | 11 |
| AccII | CGCG | 1 cut(s) | 176 |
| AciI | CCGC | 9 cut(s) | 6, 9, 131, 174, 227, 392, 425, 573, 576 |
| AclWI | GGATC | 1 cut(s) | 610 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcsI | RAATTY | 1 cut(s) | 218 |
| AcyI | GRCGYC | 2 cut(s) | 156, 236 |
| AfaI | GTAC | 2 cut(s) | 47, 313 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 26 |
| AgeI | ACCGGT | 1 cut(s) | 251 |
| AjnI | CCWGG | 1 cut(s) | 197 |
| AluBI | AGCT | 2 cut(s) | 183, 189 |
| AluI | AGCT | 2 cut(s) | 183, 189 |
| Alw26I | GTCTC | 3 cut(s) | 227, 471, 519 |
| AlwI | GGATC | 1 cut(s) | 610 |
| Ama87I | CYCGRG | 1 cut(s) | 230 |
| AoxI | GGCC | 3 cut(s) | 3, 213, 349 |
| ApoI | RAATTY | 1 cut(s) | 218 |
| AsiGI | ACCGGT | 1 cut(s) | 251 |
| AspLEI | GCGC | 1 cut(s) | 208 |
| AspS9I | GGNCC | 2 cut(s) | 249, 410 |
| AsuHPI | GGTGA | 4 cut(s) | 9, 314, 548, 595 |
| AvaI | CYCGRG | 1 cut(s) | 230 |
| AvaII | GGWCC | 2 cut(s) | 249, 410 |
| BauI | CACGAG | 1 cut(s) | 209 |
| BbsI | GAAGAC | 1 cut(s) | 258 |
| BccI | CCATC | 5 cut(s) | 71, 131, 196, 527, 622 |
| BciT130I | CCWGG | 1 cut(s) | 199 |
| BcoDI | GTCTC | 3 cut(s) | 227, 471, 519 |
| BisI | GCNGC | 6 cut(s) | 6, 9, 131, 227, 426, 574 |
| BlsI | GCNGC | 6 cut(s) | 7, 10, 132, 228, 427, 575 |
| Bme1390I | CCNGG | 1 cut(s) | 199 |
| Bme18I | GGWCC | 2 cut(s) | 249, 410 |
| BmeT110I | CYCGRG | 1 cut(s) | 230 |
| BmgT120I | GGNCC | 2 cut(s) | 249, 410 |
| BmiI | GGNNCC | 1 cut(s) | 331 |
| BmrFI | CCNGG | 1 cut(s) | 199 |
| BmsI | GCATC | 1 cut(s) | 66 |
| BpiI | GAAGAC | 1 cut(s) | 258 |
| BpmI | CTGGAG | 2 cut(s) | 52, 543 |
| BpuEI | CTTGAG | 1 cut(s) | 169 |
| BsaHI | GRCGYC | 2 cut(s) | 156, 236 |
| BsaI | GGTCTC | 1 cut(s) | 471 |
| BsaJI | CCNNGG | 2 cut(s) | 103, 346 |
| BsaWI | WCCGGW | 1 cut(s) | 251 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 26 |
| Bse118I | RCCGGY | 1 cut(s) | 251 |
| BseBI | CCWGG | 1 cut(s) | 199 |
| BseDI | CCNNGG | 2 cut(s) | 103, 346 |
| BseGI | GGATG | 2 cut(s) | 207, 400 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 26 |
| BseMII | CTCAG | 1 cut(s) | 263 |
| BseRI | GAGGAG | 1 cut(s) | 92 |
| BseYI | CCCAGC | 1 cut(s) | 509 |
| Bsh1236I | CGCG | 1 cut(s) | 176 |
| Bsh1285I | CGRYCG | 1 cut(s) | 174 |
| BshFI | GGCC | 3 cut(s) | 5, 215, 351 |
| BshTI | ACCGGT | 1 cut(s) | 251 |
| BsiEI | CGRYCG | 1 cut(s) | 174 |
| BsiHKCI | CYCGRG | 1 cut(s) | 230 |
| BsiSI | CCGG | 4 cut(s) | 27, 134, 159, 252 |
| BsiWI | CGTACG | 1 cut(s) | 45 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 26 |
| BsmAI | GTCTC | 3 cut(s) | 227, 471, 519 |
| BsmBI | CGTCTC | 1 cut(s) | 227 |
| BsnI | GGCC | 3 cut(s) | 5, 215, 351 |
| Bso31I | GGTCTC | 1 cut(s) | 471 |
| BsoBI | CYCGRG | 1 cut(s) | 230 |
| Bsp143I | GATC | 2 cut(s) | 177, 602 |
| BspACI | CCGC | 9 cut(s) | 6, 9, 131, 174, 227, 392, 425, 573, 576 |
| BspANI | GGCC | 3 cut(s) | 5, 215, 351 |
| BspCNI | CTCAG | 1 cut(s) | 264 |
| BspFNI | CGCG | 1 cut(s) | 176 |
| BspLI | GGNNCC | 1 cut(s) | 331 |
| BspPI | GGATC | 1 cut(s) | 610 |
| BspTNI | GGTCTC | 1 cut(s) | 471 |
| BsrBI | CCGCTC | 1 cut(s) | 11 |
| BsrFI | RCCGGY | 1 cut(s) | 251 |
| BssAI | RCCGGY | 1 cut(s) | 251 |
| BssECI | CCNNGG | 2 cut(s) | 103, 346 |
| BssMI | GATC | 2 cut(s) | 177, 602 |
| BssNI | GRCGYC | 2 cut(s) | 156, 236 |
| BssSI | CACGAG | 1 cut(s) | 209 |
| BssT1I | CCWWGG | 1 cut(s) | 346 |
| Bst2BI | CACGAG | 1 cut(s) | 209 |
| Bst2UI | CCWGG | 1 cut(s) | 199 |
| Bst4CI | ACNGT | 3 cut(s) | 311, 316, 530 |
| BstACI | GRCGYC | 2 cut(s) | 156, 236 |
| BstDEI | CTNAG | 1 cut(s) | 272 |
| BstF5I | GGATG | 2 cut(s) | 207, 400 |
| BstFNI | CGCG | 1 cut(s) | 176 |
| BstHHI | GCGC | 1 cut(s) | 208 |
| BstKTI | GATC | 2 cut(s) | 180, 605 |
| BstMAI | GTCTC | 3 cut(s) | 227, 471, 519 |
| BstMBI | GATC | 2 cut(s) | 177, 602 |
| BstMCI | CGRYCG | 1 cut(s) | 174 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 212, 496 |
| BstNI | CCWGG | 1 cut(s) | 199 |
| BstSCI | CCNGG | 1 cut(s) | 197 |
| BstUI | CGCG | 1 cut(s) | 176 |
| BstV2I | GAAGAC | 1 cut(s) | 258 |
| BsuRI | GGCC | 3 cut(s) | 5, 215, 351 |
| BtsCI | GGATG | 2 cut(s) | 207, 400 |
| BtsIMutI | CAGTG | 2 cut(s) | 535, 600 |
| CfoI | GCGC | 1 cut(s) | 208 |
| Cfr10I | RCCGGY | 1 cut(s) | 251 |
| Cfr13I | GGNCC | 2 cut(s) | 249, 410 |
| CseI | GACGC | 1 cut(s) | 164 |
| Csp6I | GTAC | 2 cut(s) | 46, 312 |
| CspAI | ACCGGT | 1 cut(s) | 251 |
| CviAII | CATG | 1 cut(s) | 478 |
| CviQI | GTAC | 2 cut(s) | 46, 312 |
| DdeI | CTNAG | 1 cut(s) | 272 |
| DpnI | GATC | 2 cut(s) | 179, 604 |
| DpnII | GATC | 2 cut(s) | 177, 602 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| EciI | GGCGGA | 1 cut(s) | 407 |
| Eco130I | CCWWGG | 1 cut(s) | 346 |
| Eco31I | GGTCTC | 1 cut(s) | 471 |
| Eco32I | GATATC | 1 cut(s) | 541 |
| Eco47I | GGWCC | 2 cut(s) | 249, 410 |
| Eco88I | CYCGRG | 1 cut(s) | 230 |
| EcoO109I | RGGNCCY | 1 cut(s) | 410 |
| EcoRII | CCWGG | 1 cut(s) | 197 |
| EcoRV | GATATC | 1 cut(s) | 541 |
| EcoT14I | CCWWGG | 1 cut(s) | 346 |
| ErhI | CCWWGG | 1 cut(s) | 346 |
| Esp3I | CGTCTC | 1 cut(s) | 227 |
| FaeI | CATG | 1 cut(s) | 481 |
| FaiI | YATR | 4 cut(s) | 83, 111, 385, 479 |
| FatI | CATG | 1 cut(s) | 477 |
| Fnu4HI | GCNGC | 6 cut(s) | 6, 9, 131, 227, 426, 574 |
| FokI | GGATG | 2 cut(s) | 214, 407 |
| Fsp4HI | GCNGC | 6 cut(s) | 6, 9, 131, 227, 426, 574 |
| GlaI | GCGC | 1 cut(s) | 207 |
| GluI | GCNGC | 6 cut(s) | 6, 9, 131, 227, 426, 574 |
| GsaI | CCCAGC | 1 cut(s) | 513 |
| GsuI | CTGGAG | 2 cut(s) | 52, 543 |
| HaeIII | GGCC | 3 cut(s) | 5, 215, 351 |
| HapII | CCGG | 4 cut(s) | 27, 134, 159, 252 |
| HgaI | GACGC | 1 cut(s) | 164 |
| HhaI | GCGC | 1 cut(s) | 208 |
| Hin1I | GRCGYC | 2 cut(s) | 156, 236 |
| Hin1II | CATG | 1 cut(s) | 481 |
| Hin6I | GCGC | 1 cut(s) | 206 |
| HinP1I | GCGC | 1 cut(s) | 206 |
| HincII | GTYRAC | 1 cut(s) | 115 |
| HindII | GTYRAC | 1 cut(s) | 115 |
| HpaII | CCGG | 4 cut(s) | 27, 134, 159, 252 |
| HphI | GGTGA | 4 cut(s) | 9, 314, 548, 595 |
| Hpy166II | GTNNAC | 2 cut(s) | 94, 115 |
| Hpy188I | TCNGA | 2 cut(s) | 241, 454 |
| Hpy188III | TCNNGA | 1 cut(s) | 148 |
| Hpy8I | GTNNAC | 2 cut(s) | 94, 115 |
| Hpy99I | CGWCG | 5 cut(s) | 46, 158, 172, 311, 407 |
| HpyAV | CCTTC | 3 cut(s) | 24, 343, 362 |
| HpyCH4III | ACNGT | 3 cut(s) | 311, 316, 530 |
| HpyCH4IV | ACGT | 3 cut(s) | 44, 48, 236 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 212, 496 |
| HpyF3I | CTNAG | 1 cut(s) | 272 |
| HpySE526I | ACGT | 3 cut(s) | 44, 48, 236 |
| Hsp92I | GRCGYC | 2 cut(s) | 156, 236 |
| Hsp92II | CATG | 1 cut(s) | 481 |
| HspAI | GCGC | 1 cut(s) | 206 |
| Kzo9I | GATC | 2 cut(s) | 177, 602 |
| LmnI | GCTCC | 3 cut(s) | 16, 125, 335 |
| LweI | GCATC | 1 cut(s) | 66 |
| MaeII | ACGT | 3 cut(s) | 44, 48, 236 |
| MaeIII | GTNAC | 1 cut(s) | 302 |
| MalI | GATC | 2 cut(s) | 179, 604 |
| MbiI | CCGCTC | 1 cut(s) | 11 |
| MboI | GATC | 2 cut(s) | 177, 602 |
| MboII | GAAGA | 2 cut(s) | 258, 602 |
| MluCI | AATT | 1 cut(s) | 218 |
| MmeI | TCCRAC | 1 cut(s) | 346 |
| MseI | TTAA | 1 cut(s) | 222 |
| MslI | CAYNNNNRTG | 1 cut(s) | 482 |
| MspI | CCGG | 4 cut(s) | 27, 134, 159, 252 |
| MspR9I | CCNGG | 1 cut(s) | 199 |
| MvaI | CCWGG | 1 cut(s) | 199 |
| MvnI | CGCG | 1 cut(s) | 176 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 212, 496 |
| NdeII | GATC | 2 cut(s) | 177, 602 |
| NlaIII | CATG | 1 cut(s) | 481 |
| NlaIV | GGNNCC | 1 cut(s) | 331 |
| NmeAIII | GCCGAG | 2 cut(s) | 84, 525 |
| NmuCI | GTSAC | 1 cut(s) | 302 |
| Pfl23II | CGTACG | 1 cut(s) | 45 |
| PinAI | ACCGGT | 1 cut(s) | 251 |
| PkrI | GCNGC | 6 cut(s) | 7, 10, 132, 228, 427, 575 |
| PpuMI | RGGWCCY | 1 cut(s) | 410 |
| Psp5II | RGGWCCY | 1 cut(s) | 410 |
| Psp6I | CCWGG | 1 cut(s) | 197 |
| PspFI | CCCAGC | 1 cut(s) | 509 |
| PspGI | CCWGG | 1 cut(s) | 197 |
| PspLI | CGTACG | 1 cut(s) | 45 |
| PspN4I | GGNNCC | 1 cut(s) | 331 |
| PspPI | GGNCC | 2 cut(s) | 249, 410 |
| PspPPI | RGGWCCY | 1 cut(s) | 410 |
| RsaI | GTAC | 2 cut(s) | 47, 313 |
| RsaNI | GTAC | 2 cut(s) | 46, 312 |
| RseI | CAYNNNNRTG | 1 cut(s) | 482 |
| SaqAI | TTAA | 1 cut(s) | 222 |
| SatI | GCNGC | 6 cut(s) | 6, 9, 131, 227, 426, 574 |
| Sau3AI | GATC | 2 cut(s) | 177, 602 |
| Sau96I | GGNCC | 2 cut(s) | 249, 410 |
| ScrFI | CCNGG | 1 cut(s) | 199 |
| SfaNI | GCATC | 1 cut(s) | 66 |
| SinI | GGWCC | 2 cut(s) | 249, 410 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 482 |
| SmlI | CTYRAG | 1 cut(s) | 184 |
| SmoI | CTYRAG | 1 cut(s) | 184 |
| Sse9I | AATT | 1 cut(s) | 218 |
| SsiI | CCGC | 9 cut(s) | 6, 9, 131, 174, 227, 392, 425, 573, 576 |
| StyD4I | CCNGG | 1 cut(s) | 197 |
| StyI | CCWWGG | 1 cut(s) | 346 |
| TaaI | ACNGT | 3 cut(s) | 311, 316, 530 |
| TaiI | ACGT | 3 cut(s) | 47, 51, 239 |
| TaqI | TCGA | 3 cut(s) | 319, 402, 549 |
| TasI | AATT | 1 cut(s) | 218 |
| TauI | GCSGC | 6 cut(s) | 8, 11, 133, 229, 428, 576 |
| Tru1I | TTAA | 1 cut(s) | 222 |
| Tru9I | TTAA | 1 cut(s) | 222 |
| TscAI | CASTG | 2 cut(s) | 535, 600 |
| TseFI | GTSAC | 1 cut(s) | 302 |
| Tsp45I | GTSAC | 1 cut(s) | 302 |
| TspDTI | ATGAA | 1 cut(s) | 482 |
| TspGWI | ACGGA | 1 cut(s) | 420 |
| TspRI | CASTG | 2 cut(s) | 535, 600 |
| VpaK11BI | GGWCC | 2 cut(s) | 249, 410 |
| XapI | RAATTY | 1 cut(s) | 218 |
| ZraI | GACGTC | 1 cut(s) | 237 |