Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1016 |
| AatII | GACGTC | 1 cut(s) | 177 |
| AccB1I | GGYRCC | 1 cut(s) | 508 |
| AccBSI | CCGCTC | 2 cut(s) | 254, 1579 |
| AciI | CCGC | 4 cut(s) | 252, 287, 617, 1577 |
| AclWI | GGATC | 3 cut(s) | 401, 414, 1489 |
| AcoI | YGGCCR | 1 cut(s) | 813 |
| AcsI | RAATTY | 4 cut(s) | 737, 1380, 1522, 1611 |
| AcuI | CTGAAG | 1 cut(s) | 1341 |
| AcyI | GRCGYC | 2 cut(s) | 123, 174 |
| AdeI | CACNNNGTG | 1 cut(s) | 781 |
| AfaI | GTAC | 1 cut(s) | 597 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 57, 90, 555, 1324, 1615 |
| AgsI | TTSAA | 4 cut(s) | 196, 365, 1096, 1424 |
| AjnI | CCWGG | 2 cut(s) | 138, 1351 |
| AluBI | AGCT | 8 cut(s) | 339, 361, 910, 920, 944, 949, 959, 1193 |
| AluI | AGCT | 8 cut(s) | 339, 361, 910, 920, 944, 949, 959, 1193 |
| Alw21I | GWGCWC | 2 cut(s) | 912, 983 |
| Alw26I | GTCTC | 6 cut(s) | 59, 184, 857, 1184, 1400, 1599 |
| AlwI | GGATC | 3 cut(s) | 401, 414, 1489 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 145 |
| AoxI | GGCC | 5 cut(s) | 135, 142, 311, 557, 813 |
| ApeKI | GCWGC | 4 cut(s) | 188, 920, 1190, 1193 |
| ApoI | RAATTY | 4 cut(s) | 737, 1380, 1522, 1611 |
| AspLEI | GCGC | 1 cut(s) | 63 |
| AspS9I | GGNCC | 3 cut(s) | 136, 142, 1145 |
| AsuHPI | GGTGA | 9 cut(s) | 67, 182, 319, 644, 787, 904, 1241, 1351, 1459 |
| AsuII | TTCGAA | 1 cut(s) | 434 |
| AvaII | GGWCC | 1 cut(s) | 1145 |
| AxyI | CCTNAGG | 2 cut(s) | 513, 550 |
| BaeGI | GKGCMC | 1 cut(s) | 511 |
| BamHI | GGATCC | 1 cut(s) | 406 |
| BanI | GGYRCC | 1 cut(s) | 508 |
| BanII | GRGCYC | 1 cut(s) | 912 |
| BbsI | GAAGAC | 1 cut(s) | 1107 |
| Bbv12I | GWGCWC | 2 cut(s) | 912, 983 |
| BbvI | GCAGC | 4 cut(s) | 175, 907, 1180, 1202 |
| BccI | CCATC | 4 cut(s) | 264, 764, 805, 1103 |
| BceAI | ACGGC | 1 cut(s) | 136 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 94, 128 |
| BciT130I | CCWGG | 2 cut(s) | 140, 1353 |
| BclI | TGATCA | 1 cut(s) | 1647 |
| BcoDI | GTCTC | 6 cut(s) | 59, 184, 857, 1184, 1400, 1599 |
| BfaI | CTAG | 3 cut(s) | 605, 695, 1140 |
| BfmI | CTRYAG | 2 cut(s) | 246, 1194 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 812 |
| BglII | AGATCT | 1 cut(s) | 1098 |
| BisI | GCNGC | 5 cut(s) | 189, 252, 921, 1191, 1194 |
| BlpI | GCTNAGC | 1 cut(s) | 945 |
| BlsI | GCNGC | 5 cut(s) | 190, 253, 922, 1192, 1195 |
| Bme1390I | CCNGG | 2 cut(s) | 140, 1353 |
| Bme18I | GGWCC | 1 cut(s) | 1145 |
| BmgT120I | GGNCC | 3 cut(s) | 136, 142, 1145 |
| BmiI | GGNNCC | 3 cut(s) | 408, 510, 547 |
| BmrFI | CCNGG | 2 cut(s) | 140, 1353 |
| BmrI | ACTGGG | 2 cut(s) | 164, 1539 |
| BmsI | GCATC | 6 cut(s) | 475, 497, 733, 1146, 1612, 1642 |
| BmuI | ACTGGG | 2 cut(s) | 164, 1539 |
| BoxI | GACNNNNGTC | 2 cut(s) | 163, 1392 |
| BpiI | GAAGAC | 1 cut(s) | 1107 |
| BpmI | CTGGAG | 1 cut(s) | 146 |
| Bpu10I | CCTNAGC | 1 cut(s) | 38 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 945 |
| Bpu14I | TTCGAA | 1 cut(s) | 434 |
| BsaHI | GRCGYC | 2 cut(s) | 123, 174 |
| BsaI | GGTCTC | 1 cut(s) | 1599 |
| BsaJI | CCNNGG | 4 cut(s) | 69, 138, 139, 414 |
| BsaWI | WCCGGW | 1 cut(s) | 167 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 144, 164, 174, 194 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 57, 90, 555, 1324, 1615 |
| Bse118I | RCCGGY | 3 cut(s) | 90, 101, 1361 |
| Bse1I | ACTGG | 8 cut(s) | 51, 159, 163, 1172, 1442, 1488, 1534, 1594 |
| Bse21I | CCTNAGG | 2 cut(s) | 513, 550 |
| Bse3DI | GCAATG | 6 cut(s) | 183, 303, 324, 705, 1213, 1448 |
| BseBI | CCWGG | 2 cut(s) | 140, 1353 |
| BseDI | CCNNGG | 4 cut(s) | 69, 138, 139, 414 |
| BseGI | GGATG | 3 cut(s) | 490, 972, 1348 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 57, 90, 555, 1324, 1615 |
| BseMI | GCAATG | 6 cut(s) | 183, 303, 324, 705, 1213, 1448 |
| BseMII | CTCAG | 6 cut(s) | 27, 29, 504, 564, 969, 1053 |
| BseNI | ACTGG | 8 cut(s) | 51, 159, 163, 1172, 1442, 1488, 1534, 1594 |
| BseRI | GAGGAG | 2 cut(s) | 246, 1071 |
| BseSI | GKGCMC | 1 cut(s) | 511 |
| BseXI | GCAGC | 4 cut(s) | 175, 907, 1180, 1202 |
| BsgI | GTGCAG | 1 cut(s) | 1087 |
| Bsh1285I | CGRYCG | 1 cut(s) | 621 |
| BshFI | GGCC | 5 cut(s) | 137, 144, 313, 559, 815 |
| BshNI | GGYRCC | 1 cut(s) | 508 |
| BsiEI | CGRYCG | 1 cut(s) | 621 |
| BsiHKAI | GWGCWC | 2 cut(s) | 912, 983 |
| BsiSI | CCGG | 6 cut(s) | 58, 84, 91, 102, 168, 1362 |
| BslFI | GGGAC | 3 cut(s) | 171, 586, 785 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 57, 90, 555, 1324, 1615 |
| BsmAI | GTCTC | 6 cut(s) | 59, 184, 857, 1184, 1400, 1599 |
| BsmBI | CGTCTC | 2 cut(s) | 184, 1400 |
| BsmFI | GGGAC | 3 cut(s) | 171, 586, 785 |
| BsnI | GGCC | 5 cut(s) | 137, 144, 313, 559, 815 |
| Bso31I | GGTCTC | 1 cut(s) | 1599 |
| Bsp119I | TTCGAA | 1 cut(s) | 434 |
| Bsp1286I | GDGCHC | 3 cut(s) | 511, 912, 983 |
| Bsp143I | GATC | 6 cut(s) | 406, 1021, 1045, 1098, 1494, 1647 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 945 |
| BspACI | CCGC | 4 cut(s) | 252, 287, 617, 1577 |
| BspANI | GGCC | 5 cut(s) | 137, 144, 313, 559, 815 |
| BspCNI | CTCAG | 6 cut(s) | 26, 30, 505, 563, 968, 1052 |
| BspHI | TCATGA | 1 cut(s) | 996 |
| BspLI | GGNNCC | 3 cut(s) | 408, 510, 547 |
| BspMAI | CTGCAG | 1 cut(s) | 1198 |
| BspPI | GGATC | 3 cut(s) | 401, 414, 1489 |
| BspQI | GCTCTTC | 1 cut(s) | 988 |
| BspT104I | TTCGAA | 1 cut(s) | 434 |
| BspT107I | GGYRCC | 1 cut(s) | 508 |
| BspTNI | GGTCTC | 1 cut(s) | 1599 |
| BsrBI | CCGCTC | 2 cut(s) | 254, 1579 |
| BsrDI | GCAATG | 6 cut(s) | 183, 303, 324, 705, 1213, 1448 |
| BsrFI | RCCGGY | 3 cut(s) | 90, 101, 1361 |
| BsrI | ACTGG | 8 cut(s) | 51, 159, 163, 1172, 1442, 1488, 1534, 1594 |
| BssAI | RCCGGY | 3 cut(s) | 90, 101, 1361 |
| BssECI | CCNNGG | 4 cut(s) | 69, 138, 139, 414 |
| BssMI | GATC | 6 cut(s) | 406, 1021, 1045, 1098, 1494, 1647 |
| BssNI | GRCGYC | 2 cut(s) | 123, 174 |
| Bst2UI | CCWGG | 2 cut(s) | 140, 1353 |
| Bst4CI | ACNGT | 9 cut(s) | 79, 200, 503, 600, 632, 709, 799, 868, 1511 |
| Bst6I | CTCTTC | 4 cut(s) | 276, 988, 1317, 1597 |
| BstACI | GRCGYC | 2 cut(s) | 123, 174 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 400, 614 |
| BstBI | TTCGAA | 1 cut(s) | 434 |
| BstC8I | GCNNGC | 5 cut(s) | 146, 315, 718, 1073, 1077 |
| BstDSI | CCRYGG | 1 cut(s) | 69 |
| BstEII | GGTNACC | 1 cut(s) | 73 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 553 |
| BstF5I | GGATG | 3 cut(s) | 490, 972, 1348 |
| BstHHI | GCGC | 1 cut(s) | 63 |
| BstKTI | GATC | 6 cut(s) | 409, 1024, 1048, 1101, 1497, 1650 |
| BstMAI | GTCTC | 6 cut(s) | 59, 184, 857, 1184, 1400, 1599 |
| BstMBI | GATC | 6 cut(s) | 406, 1021, 1045, 1098, 1494, 1647 |
| BstMCI | CGRYCG | 1 cut(s) | 621 |
| BstNI | CCWGG | 2 cut(s) | 140, 1353 |
| BstNSI | RCATGY | 2 cut(s) | 398, 720 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 163, 1392 |
| BstPI | GGTNACC | 1 cut(s) | 73 |
| BstSCI | CCNGG | 2 cut(s) | 138, 1351 |
| BstSFI | CTRYAG | 2 cut(s) | 246, 1194 |
| BstSLI | GKGCMC | 1 cut(s) | 511 |
| BstV1I | GCAGC | 4 cut(s) | 175, 907, 1180, 1202 |
| BstV2I | GAAGAC | 1 cut(s) | 1107 |
| BstX2I | RGATCY | 3 cut(s) | 406, 1098, 1494 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 677 |
| BstYI | RGATCY | 3 cut(s) | 406, 1098, 1494 |
| Bsu36I | CCTNAGG | 2 cut(s) | 513, 550 |
| BsuRI | GGCC | 5 cut(s) | 137, 144, 313, 559, 815 |
| BtgI | CCRYGG | 1 cut(s) | 69 |
| BtsCI | GGATG | 3 cut(s) | 490, 972, 1348 |
| BtsI | GCAGTG | 1 cut(s) | 31 |
| BtsIMutI | CAGTG | 4 cut(s) | 31, 205, 986, 1495 |
| Cac8I | GCNNGC | 5 cut(s) | 146, 315, 718, 1073, 1077 |
| CaiI | CAGNNNCTG | 1 cut(s) | 145 |
| CciI | TCATGA | 1 cut(s) | 996 |
| CfoI | GCGC | 1 cut(s) | 63 |
| Cfr10I | RCCGGY | 3 cut(s) | 90, 101, 1361 |
| Cfr13I | GGNCC | 3 cut(s) | 136, 142, 1145 |
| CseI | GACGC | 2 cut(s) | 112, 1397 |
| Csp6I | GTAC | 1 cut(s) | 596 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 285, 320 |
| CviAII | CATG | 9 cut(s) | 127, 348, 395, 717, 722, 808, 997, 1085, 1151 |
| CviQI | GTAC | 1 cut(s) | 596 |
| DpnI | GATC | 6 cut(s) | 408, 1023, 1047, 1100, 1496, 1649 |
| DpnII | GATC | 6 cut(s) | 406, 1021, 1045, 1098, 1494, 1647 |
| DraI | TTTAAA | 1 cut(s) | 1537 |
| DraIII | CACNNNGTG | 1 cut(s) | 781 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1016 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1016 |
| EaeI | YGGCCR | 1 cut(s) | 813 |
| Eam1104I | CTCTTC | 4 cut(s) | 276, 988, 1317, 1597 |
| EarI | CTCTTC | 4 cut(s) | 276, 988, 1317, 1597 |
| Ecl136II | GAGCTC | 1 cut(s) | 910 |
| Eco147I | AGGCCT | 1 cut(s) | 313 |
| Eco24I | GRGCYC | 1 cut(s) | 912 |
| Eco31I | GGTCTC | 1 cut(s) | 1599 |
| Eco47I | GGWCC | 1 cut(s) | 1145 |
| Eco53kI | GAGCTC | 1 cut(s) | 910 |
| Eco57I | CTGAAG | 1 cut(s) | 1341 |
| Eco81I | CCTNAGG | 2 cut(s) | 513, 550 |
| Eco91I | GGTNACC | 1 cut(s) | 73 |
| EcoICRI | GAGCTC | 1 cut(s) | 910 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 553 |
| EcoO109I | RGGNCCY | 2 cut(s) | 142, 1145 |
| EcoO65I | GGTNACC | 1 cut(s) | 73 |
| EcoRI | GAATTC | 1 cut(s) | 1380 |
| EcoRII | CCWGG | 2 cut(s) | 138, 1351 |
| EcoT22I | ATGCAT | 2 cut(s) | 490, 860 |
| EcoT38I | GRGCYC | 1 cut(s) | 912 |
| Esp3I | CGTCTC | 2 cut(s) | 184, 1400 |
| FaeI | CATG | 9 cut(s) | 130, 351, 398, 720, 725, 811, 1000, 1088, 1154 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 933, 965 |
| FaqI | GGGAC | 3 cut(s) | 171, 586, 785 |
| FatI | CATG | 9 cut(s) | 126, 347, 394, 716, 721, 807, 996, 1084, 1150 |
| FauNDI | CATATG | 1 cut(s) | 235 |
| FbaI | TGATCA | 1 cut(s) | 1647 |
| Fnu4HI | GCNGC | 5 cut(s) | 189, 252, 921, 1191, 1194 |
| FokI | GGATG | 3 cut(s) | 497, 979, 1335 |
| FriOI | GRGCYC | 1 cut(s) | 912 |
| Fsp4HI | GCNGC | 5 cut(s) | 189, 252, 921, 1191, 1194 |
| FspBI | CTAG | 3 cut(s) | 605, 695, 1140 |
| GlaI | GCGC | 1 cut(s) | 62 |
| GluI | GCNGC | 5 cut(s) | 189, 252, 921, 1191, 1194 |
| GsuI | CTGGAG | 1 cut(s) | 146 |
| HaeIII | GGCC | 5 cut(s) | 137, 144, 313, 559, 815 |
| HapII | CCGG | 6 cut(s) | 58, 84, 91, 102, 168, 1362 |
| HgaI | GACGC | 2 cut(s) | 112, 1397 |
| HhaI | GCGC | 1 cut(s) | 63 |
| Hin1I | GRCGYC | 2 cut(s) | 123, 174 |
| Hin1II | CATG | 9 cut(s) | 130, 351, 398, 720, 725, 811, 1000, 1088, 1154 |
| Hin6I | GCGC | 1 cut(s) | 61 |
| HinP1I | GCGC | 1 cut(s) | 61 |
| HindIII | AAGCTT | 2 cut(s) | 359, 947 |
| HinfI | GANTC | 5 cut(s) | 159, 1199, 1239, 1290, 1356 |
| HpaII | CCGG | 6 cut(s) | 58, 84, 91, 102, 168, 1362 |
| HphI | GGTGA | 9 cut(s) | 67, 182, 319, 644, 787, 904, 1241, 1351, 1459 |
| Hpy166II | GTNNAC | 1 cut(s) | 118 |
| Hpy188I | TCNGA | 4 cut(s) | 16, 331, 418, 1120 |
| Hpy188III | TCNNGA | 6 cut(s) | 543, 552, 871, 997, 1294, 1385 |
| Hpy8I | GTNNAC | 1 cut(s) | 118 |
| Hpy99I | CGWCG | 2 cut(s) | 83, 179 |
| HpyAV | CCTTC | 5 cut(s) | 202, 390, 420, 549, 1003 |
| HpyCH4III | ACNGT | 9 cut(s) | 79, 200, 503, 600, 632, 709, 799, 868, 1511 |
| HpyCH4IV | ACGT | 3 cut(s) | 174, 977, 1260 |
| HpySE526I | ACGT | 3 cut(s) | 174, 977, 1260 |
| Hsp92I | GRCGYC | 2 cut(s) | 123, 174 |
| Hsp92II | CATG | 9 cut(s) | 130, 351, 398, 720, 725, 811, 1000, 1088, 1154 |
| HspAI | GCGC | 1 cut(s) | 61 |
| Ksp22I | TGATCA | 1 cut(s) | 1647 |
| Kzo9I | GATC | 6 cut(s) | 406, 1021, 1045, 1098, 1494, 1647 |
| LguI | GCTCTTC | 1 cut(s) | 988 |
| LmnI | GCTCC | 5 cut(s) | 153, 259, 664, 713, 1084 |
| Lsp1109I | GCAGC | 4 cut(s) | 175, 907, 1180, 1202 |
| LweI | GCATC | 6 cut(s) | 475, 497, 733, 1146, 1612, 1642 |
| MaeI | CTAG | 3 cut(s) | 605, 695, 1140 |
| MaeII | ACGT | 3 cut(s) | 174, 977, 1260 |
| MaeIII | GTNAC | 8 cut(s) | 73, 170, 219, 424, 781, 1229, 1256, 1357 |
| MalI | GATC | 6 cut(s) | 408, 1023, 1047, 1100, 1496, 1649 |
| MbiI | CCGCTC | 2 cut(s) | 254, 1579 |
| MboI | GATC | 6 cut(s) | 406, 1021, 1045, 1098, 1494, 1647 |
| MboII | GAAGA | 9 cut(s) | 293, 650, 886, 975, 1107, 1108, 1334, 1576, 1614 |
| MfeI | CAATTG | 2 cut(s) | 228, 903 |
| MflI | RGATCY | 3 cut(s) | 406, 1098, 1494 |
| MhlI | GDGCHC | 3 cut(s) | 511, 912, 983 |
| MlyI | GAGTC | 3 cut(s) | 153, 1208, 1365 |
| MmeI | TCCRAC | 2 cut(s) | 175, 691 |
| Mph1103I | ATGCAT | 2 cut(s) | 490, 860 |
| MseI | TTAA | 6 cut(s) | 261, 441, 449, 881, 1136, 1536 |
| MslI | CAYNNNNRTG | 3 cut(s) | 675, 853, 1403 |
| MspA1I | CMGCKG | 1 cut(s) | 1193 |
| MspI | CCGG | 6 cut(s) | 58, 84, 91, 102, 168, 1362 |
| MspR9I | CCNGG | 2 cut(s) | 140, 1353 |
| MunI | CAATTG | 2 cut(s) | 228, 903 |
| MvaI | CCWGG | 2 cut(s) | 140, 1353 |
| NdeI | CATATG | 1 cut(s) | 235 |
| NdeII | GATC | 6 cut(s) | 406, 1021, 1045, 1098, 1494, 1647 |
| NlaIII | CATG | 9 cut(s) | 130, 351, 398, 720, 725, 811, 1000, 1088, 1154 |
| NlaIV | GGNNCC | 3 cut(s) | 408, 510, 547 |
| NmuCI | GTSAC | 5 cut(s) | 73, 170, 781, 1229, 1357 |
| NsiI | ATGCAT | 2 cut(s) | 490, 860 |
| NspI | RCATGY | 2 cut(s) | 398, 720 |
| NspV | TTCGAA | 1 cut(s) | 434 |
| PaeI | GCATGC | 1 cut(s) | 720 |
| PagI | TCATGA | 1 cut(s) | 996 |
| PasI | CCCWGGG | 1 cut(s) | 139 |
| PceI | AGGCCT | 1 cut(s) | 313 |
| PciSI | GCTCTTC | 1 cut(s) | 988 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 120 |
| PfeI | GAWTC | 2 cut(s) | 1239, 1290 |
| PflFI | GACNNNGTC | 2 cut(s) | 122, 176 |
| PfoI | TCCNGGA | 1 cut(s) | 1351 |
| PkrI | GCNGC | 5 cut(s) | 190, 253, 922, 1192, 1195 |
| PleI | GAGTC | 3 cut(s) | 153, 1207, 1364 |
| PpsI | GAGTC | 3 cut(s) | 153, 1207, 1364 |
| PpuMI | RGGWCCY | 1 cut(s) | 1145 |
| PshAI | GACNNNNGTC | 2 cut(s) | 163, 1392 |
| Psp124BI | GAGCTC | 1 cut(s) | 912 |
| Psp5II | RGGWCCY | 1 cut(s) | 1145 |
| Psp6I | CCWGG | 2 cut(s) | 138, 1351 |
| PspEI | GGTNACC | 1 cut(s) | 73 |
| PspGI | CCWGG | 2 cut(s) | 138, 1351 |
| PspN4I | GGNNCC | 3 cut(s) | 408, 510, 547 |
| PspPI | GGNCC | 3 cut(s) | 136, 142, 1145 |
| PspPPI | RGGWCCY | 1 cut(s) | 1145 |
| PstI | CTGCAG | 1 cut(s) | 1198 |
| PstNI | CAGNNNCTG | 1 cut(s) | 145 |
| PsuI | RGATCY | 3 cut(s) | 406, 1098, 1494 |
| PsyI | GACNNNGTC | 2 cut(s) | 122, 176 |
| PvuII | CAGCTG | 1 cut(s) | 1193 |
| RsaI | GTAC | 1 cut(s) | 597 |
| RsaNI | GTAC | 1 cut(s) | 596 |
| RseI | CAYNNNNRTG | 3 cut(s) | 675, 853, 1403 |
| SacI | GAGCTC | 1 cut(s) | 912 |
| SapI | GCTCTTC | 1 cut(s) | 988 |
| SaqAI | TTAA | 6 cut(s) | 261, 441, 449, 881, 1136, 1536 |
| SatI | GCNGC | 5 cut(s) | 189, 252, 921, 1191, 1194 |
| Sau3AI | GATC | 6 cut(s) | 406, 1021, 1045, 1098, 1494, 1647 |
| Sau96I | GGNCC | 3 cut(s) | 136, 142, 1145 |
| SchI | GAGTC | 3 cut(s) | 153, 1208, 1365 |
| ScrFI | CCNGG | 2 cut(s) | 140, 1353 |
| SduI | GDGCHC | 3 cut(s) | 511, 912, 983 |
| SfaNI | GCATC | 6 cut(s) | 475, 497, 733, 1146, 1612, 1642 |
| SfcI | CTRYAG | 2 cut(s) | 246, 1194 |
| SfuI | TTCGAA | 1 cut(s) | 434 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1361 |
| SinI | GGWCC | 1 cut(s) | 1145 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 675, 853, 1403 |
| SphI | GCATGC | 1 cut(s) | 720 |
| SseBI | AGGCCT | 1 cut(s) | 313 |
| SsiI | CCGC | 4 cut(s) | 252, 287, 617, 1577 |
| SspI | AATATT | 1 cut(s) | 1643 |
| SspMI | CTAG | 3 cut(s) | 605, 695, 1140 |
| SstI | GAGCTC | 1 cut(s) | 912 |
| StuI | AGGCCT | 1 cut(s) | 313 |
| StyD4I | CCNGG | 2 cut(s) | 138, 1351 |
| TaaI | ACNGT | 9 cut(s) | 79, 200, 503, 600, 632, 709, 799, 868, 1511 |
| TaiI | ACGT | 3 cut(s) | 177, 980, 1263 |
| TaqI | TCGA | 5 cut(s) | 434, 741, 759, 1020, 1288 |
| TatI | WGTACW | 1 cut(s) | 595 |
| TauI | GCSGC | 1 cut(s) | 254 |
| TfiI | GAWTC | 2 cut(s) | 1239, 1290 |
| Tru1I | TTAA | 6 cut(s) | 261, 441, 449, 881, 1136, 1536 |
| Tru9I | TTAA | 6 cut(s) | 261, 441, 449, 881, 1136, 1536 |
| TscAI | CASTG | 4 cut(s) | 38, 205, 993, 1495 |
| TseFI | GTSAC | 5 cut(s) | 73, 170, 781, 1229, 1357 |
| TseI | GCWGC | 4 cut(s) | 188, 920, 1190, 1193 |
| Tsp45I | GTSAC | 5 cut(s) | 73, 170, 781, 1229, 1357 |
| TspDTI | ATGAA | 4 cut(s) | 294, 985, 1231, 1543 |
| TspGWI | ACGGA | 3 cut(s) | 58, 986, 1415 |
| TspRI | CASTG | 4 cut(s) | 38, 205, 993, 1495 |
| Tth111I | GACNNNGTC | 2 cut(s) | 122, 176 |
| VpaK11BI | GGWCC | 1 cut(s) | 1145 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 553 |
| XapI | RAATTY | 4 cut(s) | 737, 1380, 1522, 1611 |
| XceI | RCATGY | 2 cut(s) | 398, 720 |
| XspI | CTAG | 3 cut(s) | 605, 695, 1140 |
| ZraI | GACGTC | 1 cut(s) | 175 |
| Zsp2I | ATGCAT | 2 cut(s) | 490, 860 |