FvH4_3g22930

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000118
Physical Location & Seq
Reverse (-)
16073262 .. 16074308
1047 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22930.t1

Sequence Viewer

Length: 729 bp
ATGGCTAGAATCCTTTCCACCTCAGCCTTCTATTTGCTCATAATATTTTCCCTCACCCTGTCTTTGTTTTCCATGATCTTGGTTTCTGCAGAAGATCATGAGTTTGAGAAATCCATGGACCTCAAGCTTCTTGGTTTGAAAAAAGAAAAGCTCACCCATTTTCGCATGTATTGGGACGAAGTTGTTGATGGTCCGAATCCCTCTGCCATAACAATTGTGCAACCATCCAACTCCTCCCAAACAACATTTGGCCTCGTAAGAATGTTCGACAACCGGCTCACCCTAGGCCCCGAACGGAGCTCGAAACTTCTGGCAAGGGCTCAAGGGTTTTTTGGATCTGCTTCACAAGAAGACGTTAGCCTGCTTATGGCTCAGAACTTTGCTTTCATTCAGGGCAAGTATAACGGTAGCACCATAACCCTGATGGGGAGGAACACAGTCTTCGACAAAGTGAGGGAGTTGTCTGTGATAGGAGGAAGTGGACGTTTCAGACCGGATGTGGAGCGGACGTCCGCACTCCAGCTTAAAGTGCAGACCTCGTCTGTTCTCCGCCGTCTCACGCCGGCGACGGCTTCTCTCCTTCCCGGACCGGATGTGGAGCGGATGTCCGCACTCCGGCTTAAAGTGCAGACCTCGTCTGTTCTCCGCCGTCTCACGCCGGCGACGGCTTCTCTCCTTCCCGGACGACAGTACAGGCTTCCAGGACGGCTTCTATCCTTCCAGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

243

Amino Acids

26.91

Weight (kDa)

10.21

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 52 - 164 1.2e-39 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000118)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01361 FvH4_1g07161 FvH4_1g07431 FvH4_1g07821 FvH4_1g08223 FvH4_1g10041 FvH4_1g12132 FvH4_1g15053 FvH4_1g18161 FvH4_1g18400 FvH4_1g18931 FvH4_1g19242 FvH4_1g19461 FvH4_1g20550 FvH4_1g21381 FvH4_1g21542 FvH4_1g22102 FvH4_1g23991 FvH4_1g24542 FvH4_1g24951 FvH4_1g24960 FvH4_1g25272 FvH4_1g25552 FvH4_1g25771 FvH4_1g28771 FvH4_1g29201 FvH4_1g29611 FvH4_2g01181 FvH4_2g02491 FvH4_2g04621 FvH4_2g04631 FvH4_2g07460 FvH4_2g08540 FvH4_2g08550 FvH4_2g09292 FvH4_2g10501 FvH4_2g12101 FvH4_2g12220 FvH4_2g12551 FvH4_2g14071 FvH4_2g14241 FvH4_2g15041 FvH4_2g15311 FvH4_2g18971 FvH4_2g19220 FvH4_2g19260 FvH4_2g19611 FvH4_2g19652 FvH4_2g20211 FvH4_2g20231 FvH4_2g21541 FvH4_2g22380 FvH4_2g27712 FvH4_2g28171 FvH4_2g29879 FvH4_2g32391 FvH4_3g02171 FvH4_3g05531 FvH4_3g09750 FvH4_3g10310 FvH4_3g10460 FvH4_3g13172 FvH4_3g15232 FvH4_3g18481 FvH4_3g18621 FvH4_3g20504 FvH4_3g20812 FvH4_3g22320 FvH4_3g22930 FvH4_3g22940 FvH4_3g23752 FvH4_3g24791 FvH4_3g26411 FvH4_3g26432 FvH4_3g27201 FvH4_3g27862 FvH4_3g28871 FvH4_3g28954 FvH4_3g29131 FvH4_3g29832 FvH4_3g29922 FvH4_3g30190 FvH4_3g32231 FvH4_3g32250 FvH4_3g33460 FvH4_3g34670 FvH4_3g36982 FvH4_3g37001 FvH4_3g38252 FvH4_3g38772 FvH4_3g40141 FvH4_3g40181 FvH4_3g42521 FvH4_4g00090 FvH4_4g00290 FvH4_4g00301 FvH4_4g00530 FvH4_4g00823 FvH4_4g02300 FvH4_4g02450 FvH4_4g03360 FvH4_4g04481 FvH4_4g04761 FvH4_4g06301 FvH4_4g06630 FvH4_4g08312 FvH4_4g08552 FvH4_4g10053 FvH4_4g11971 FvH4_4g13211 FvH4_4g13511 FvH4_4g15351 FvH4_4g16360 FvH4_4g16601 FvH4_4g18462 FvH4_4g22911 FvH4_4g23221 FvH4_4g24301 FvH4_4g24721 FvH4_4g27905 FvH4_4g28971 FvH4_4g29540 FvH4_4g30720 FvH4_4g31521 FvH4_4g33371 FvH4_4g34111 FvH4_4g35740 FvH4_5g00131 FvH4_5g02530 FvH4_5g02550 FvH4_5g02560 FvH4_5g02580 FvH4_5g03783 FvH4_5g05681 FvH4_5g05681 FvH4_5g09371 FvH4_5g09381 FvH4_5g14611 FvH4_5g15341 FvH4_5g19801 FvH4_5g20110 FvH4_5g20911 FvH4_5g22230 FvH4_5g25751 FvH4_5g26070 FvH4_5g26471 FvH4_5g27072 FvH4_5g27471 FvH4_5g27471 FvH4_5g27998 FvH4_5g28291 FvH4_5g30951 FvH4_5g31082 FvH4_5g31890 FvH4_5g32251 FvH4_5g34112 FvH4_5g37184 FvH4_5g37993 FvH4_5g38271 FvH4_6g00153 FvH4_6g00640 FvH4_6g05921 FvH4_6g08591 FvH4_6g09511 FvH4_6g10331 FvH4_6g10401 FvH4_6g14161 FvH4_6g14171 FvH4_6g18481 FvH4_6g19721 FvH4_6g19980 FvH4_6g20104 FvH4_6g20112 FvH4_6g21261 FvH4_6g21651 FvH4_6g22051 FvH4_6g23141 FvH4_6g24822 FvH4_6g25082 FvH4_6g25391 FvH4_6g25560 FvH4_6g26350 FvH4_6g27031 FvH4_6g27810 FvH4_6g27971 FvH4_6g28331 FvH4_6g29391 FvH4_6g30781 FvH4_6g32221 FvH4_6g34680 FvH4_6g36181 FvH4_6g36551 FvH4_6g37491 FvH4_6g37663 FvH4_6g39590 FvH4_6g40582 FvH4_6g43843 FvH4_6g50551 FvH4_7g00240 FvH4_7g00731 FvH4_7g01031 FvH4_7g03301 FvH4_7g08180 FvH4_7g08180 FvH4_7g10631 FvH4_7g19710 FvH4_7g23892 FvH4_7g25280 FvH4_7g25551 FvH4_7g30121 FvH4_7g31931 FvH4_7g32672 FvH4_c21g00040 FvH4_c2g00053
prunus_persica Prupe.1G448800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322451 RchiOBHm_Chr5g0015171
rosa_laevigata RLG00000003077 RLG00000018186 RLG00000030343
rosa_multiflora Rmu_co8096726.1_g000001
rosa_roxburghii Rroxscaffold_157G00438380 Rroxscaffold_1G00001730 Rroxscaffold_3G00236310
rosa_rugosa Rorug02G0236500
rosa_samantha Rh4BG247300 Rh6BG286200 Rh6CG287300 Rh7DG257500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 512
AccBSI CCGCTC 2 cut(s) 505, 601
AciI CCGC 6 cut(s) 505, 513, 550, 601, 609, 646
AclWI GGATC 1 cut(s) 343
AcyI GRCGYC 1 cut(s) 509
AfaI GTAC 1 cut(s) 692
AfiI CCNNNNNNNGG 3 cut(s) 367, 426, 615
AgsI TTSAA 1 cut(s) 139
AjnI CCWGG 2 cut(s) 700, 720
AluBI AGCT 4 cut(s) 127, 151, 300, 523
AluI AGCT 4 cut(s) 127, 151, 300, 523
Alw21I GWGCWC 1 cut(s) 302
Alw26I GTCTC 2 cut(s) 560, 656
AlwI GGATC 1 cut(s) 343
AoxI GGCC 2 cut(s) 250, 286
Asp700I GAANNNNTTC 1 cut(s) 13
AspA2I CCTAGG 1 cut(s) 283
AspS9I GGNCC 4 cut(s) 118, 191, 287, 587
AsuC2I CCSGG 2 cut(s) 585, 681
AsuHPI GGTGA 3 cut(s) 46, 145, 271
AvaII GGWCC 3 cut(s) 118, 191, 587
AvrII CCTAGG 1 cut(s) 283
BanII GRGCYC 2 cut(s) 302, 322
BbsI GAAGAC 2 cut(s) 357, 433
Bbv12I GWGCWC 1 cut(s) 302
BbvCI CCTCAGC 1 cut(s) 22
BccI CCATC 3 cut(s) 182, 232, 418
BceAI ACGGC 5 cut(s) 537, 585, 633, 681, 722
BciT130I CCWGG 2 cut(s) 702, 722
BcnI CCSGG 2 cut(s) 585, 681
BcoDI GTCTC 2 cut(s) 560, 656
BfaI CTAG 2 cut(s) 6, 284
BfmI CTRYAG 1 cut(s) 87
BlnI CCTAGG 1 cut(s) 283
Bme1390I CCNGG 4 cut(s) 585, 681, 702, 722
Bme18I GGWCC 3 cut(s) 118, 191, 587
BmgT120I GGNCC 4 cut(s) 118, 191, 287, 587
BmiI GGNNCC 1 cut(s) 289
BmrFI CCNGG 4 cut(s) 585, 681, 702, 722
BpiI GAAGAC 2 cut(s) 357, 433
BpmI CTGGAG 1 cut(s) 503
Bpu10I CCTNAGC 1 cut(s) 22
BpuEI CTTGAG 2 cut(s) 107, 306
BpuMI CCSGG 2 cut(s) 585, 681
BsaHI GRCGYC 1 cut(s) 509
BsaJI CCNNGG 2 cut(s) 114, 283
BsaWI WCCGGW 2 cut(s) 493, 589
BsaXI ACNNNNNCTCC 4 cut(s) 494, 524, 590, 620
Bsc4I CCNNNNNNNGG 3 cut(s) 367, 426, 615
Bse118I RCCGGY 3 cut(s) 273, 562, 658
BseBI CCWGG 2 cut(s) 702, 722
BseDI CCNNGG 2 cut(s) 114, 283
BseGI GGATG 4 cut(s) 224, 502, 598, 609
BseLI CCNNNNNNNGG 3 cut(s) 367, 426, 615
BseMII CTCAG 2 cut(s) 36, 386
BseRI GAGGAG 1 cut(s) 223
BsgI GTGCAG 2 cut(s) 551, 647
BshFI GGCC 2 cut(s) 252, 288
BsiHKAI GWGCWC 1 cut(s) 302
BsiSI CCGG 8 cut(s) 274, 494, 563, 585, 590, 616, 659, 681
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 3 cut(s) 367, 426, 615
BsmAI GTCTC 2 cut(s) 560, 656
BsmBI CGTCTC 2 cut(s) 560, 656
BsmFI GGGAC 1 cut(s) 188
BsnI GGCC 2 cut(s) 252, 288
Bsp1286I GDGCHC 2 cut(s) 302, 322
Bsp143I GATC 3 cut(s) 75, 94, 335
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 6 cut(s) 505, 513, 550, 601, 609, 646
BspANI GGCC 2 cut(s) 252, 288
BspCNI CTCAG 2 cut(s) 35, 385
BspHI TCATGA 1 cut(s) 97
BspLI GGNNCC 1 cut(s) 289
BspMAI CTGCAG 1 cut(s) 91
BspPI GGATC 1 cut(s) 343
BsrBI CCGCTC 2 cut(s) 505, 601
BsrFI RCCGGY 3 cut(s) 273, 562, 658
BssAI RCCGGY 3 cut(s) 273, 562, 658
BssECI CCNNGG 2 cut(s) 114, 283
BssMI GATC 3 cut(s) 75, 94, 335
BssNI GRCGYC 1 cut(s) 509
BssT1I CCWWGG 2 cut(s) 114, 283
Bst2UI CCWGG 2 cut(s) 702, 722
Bst4CI ACNGT 3 cut(s) 407, 439, 690
BstACI GRCGYC 1 cut(s) 509
BstC8I GCNNGC 3 cut(s) 362, 564, 660
BstDEI CTNAG 2 cut(s) 22, 372
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 4 cut(s) 224, 502, 598, 609
BstKTI GATC 3 cut(s) 78, 97, 338
BstMAI GTCTC 2 cut(s) 560, 656
BstMBI GATC 3 cut(s) 75, 94, 335
BstMWI GCNNNNNNNGC 2 cut(s) 529, 625
BstNI CCWGG 2 cut(s) 702, 722
BstNSI RCATGY 1 cut(s) 169
BstSCI CCNGG 4 cut(s) 583, 679, 700, 720
BstSFI CTRYAG 1 cut(s) 87
BstV2I GAAGAC 2 cut(s) 357, 433
BstX2I RGATCY 1 cut(s) 335
BstXI CCANNNNNNTGG 1 cut(s) 79
BstYI RGATCY 1 cut(s) 335
BsuRI GGCC 2 cut(s) 252, 288
BtgI CCRYGG 1 cut(s) 114
BtsCI GGATG 4 cut(s) 224, 502, 598, 609
Cac8I GCNNGC 3 cut(s) 362, 564, 660
CciI TCATGA 1 cut(s) 97
Cfr10I RCCGGY 3 cut(s) 273, 562, 658
Cfr13I GGNCC 4 cut(s) 118, 191, 287, 587
CpoI CGGWCCG 1 cut(s) 587
Csp6I GTAC 1 cut(s) 691
CspI CGGWCCG 1 cut(s) 587
CviAII CATG 4 cut(s) 73, 98, 115, 166
CviQI GTAC 1 cut(s) 691
DdeI CTNAG 2 cut(s) 22, 372
DpnI GATC 3 cut(s) 77, 96, 337
DpnII GATC 3 cut(s) 75, 94, 335
EciI GGCGGA 2 cut(s) 539, 635
Ecl136II GAGCTC 1 cut(s) 300
Eco130I CCWWGG 2 cut(s) 114, 283
Eco24I GRGCYC 2 cut(s) 302, 322
Eco47I GGWCC 3 cut(s) 118, 191, 587
Eco53kI GAGCTC 1 cut(s) 300
EcoICRI GAGCTC 1 cut(s) 300
EcoO109I RGGNCCY 1 cut(s) 287
EcoRII CCWGG 2 cut(s) 700, 720
EcoT14I CCWWGG 2 cut(s) 114, 283
EcoT38I GRGCYC 2 cut(s) 302, 322
ErhI CCWWGG 2 cut(s) 114, 283
Esp3I CGTCTC 2 cut(s) 560, 656
FaeI CATG 4 cut(s) 76, 101, 118, 169
FaiI YATR 9 cut(s) 41, 74, 99, 116, 167, 209, 368, 402, 416
FaqI GGGAC 1 cut(s) 188
FatI CATG 4 cut(s) 72, 97, 114, 165
FokI GGATG 4 cut(s) 211, 509, 605, 616
FriOI GRGCYC 2 cut(s) 302, 322
FspBI CTAG 2 cut(s) 6, 284
GsuI CTGGAG 1 cut(s) 503
HaeIII GGCC 2 cut(s) 252, 288
HapII CCGG 8 cut(s) 274, 494, 563, 585, 590, 616, 659, 681
Hin1I GRCGYC 1 cut(s) 509
Hin1II CATG 4 cut(s) 76, 101, 118, 169
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 2 cut(s) 9, 196
HpaII CCGG 8 cut(s) 274, 494, 563, 585, 590, 616, 659, 681
HphI GGTGA 3 cut(s) 46, 145, 271
Hpy166II GTNNAC 1 cut(s) 482
Hpy188I TCNGA 3 cut(s) 195, 375, 491
Hpy188III TCNNGA 1 cut(s) 98
Hpy8I GTNNAC 1 cut(s) 482
Hpy99I CGWCG 2 cut(s) 571, 667
HpyAV CCTTC 4 cut(s) 37, 590, 686, 727
HpyCH4III ACNGT 3 cut(s) 407, 439, 690
HpyCH4IV ACGT 3 cut(s) 354, 484, 509
HpyCH4V TGCA 4 cut(s) 89, 220, 532, 628
HpyF10VI GCNNNNNNNGC 2 cut(s) 529, 625
HpyF3I CTNAG 2 cut(s) 22, 372
HpySE526I ACGT 3 cut(s) 354, 484, 509
Hsp92I GRCGYC 1 cut(s) 509
Hsp92II CATG 4 cut(s) 76, 101, 118, 169
KroI GCCGGC 2 cut(s) 562, 658
KroNI GCCGGC 2 cut(s) 564, 660
Kzo9I GATC 3 cut(s) 75, 94, 335
LmnI GCTCC 3 cut(s) 297, 502, 598
MaeI CTAG 2 cut(s) 6, 284
MaeII ACGT 3 cut(s) 354, 484, 509
MalI GATC 3 cut(s) 77, 96, 337
MbiI CCGCTC 2 cut(s) 505, 601
MboI GATC 3 cut(s) 75, 94, 335
MboII GAAGA 3 cut(s) 104, 362, 433
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 1 cut(s) 335
MhlI GDGCHC 2 cut(s) 302, 322
MluCI AATT 1 cut(s) 213
MmeI TCCRAC 1 cut(s) 252
MreI CGCCGGCG 2 cut(s) 562, 658
MroNI GCCGGC 2 cut(s) 562, 658
MroXI GAANNNNTTC 1 cut(s) 13
MseI TTAA 2 cut(s) 525, 621
MspI CCGG 8 cut(s) 274, 494, 563, 585, 590, 616, 659, 681
MspR9I CCNGG 4 cut(s) 585, 681, 702, 722
MunI CAATTG 1 cut(s) 213
MvaI CCWGG 2 cut(s) 702, 722
MwoI GCNNNNNNNGC 2 cut(s) 529, 625
NaeI GCCGGC 2 cut(s) 564, 660
NciI CCSGG 2 cut(s) 585, 681
NcoI CCATGG 1 cut(s) 114
NdeII GATC 3 cut(s) 75, 94, 335
NgoMIV GCCGGC 2 cut(s) 562, 658
NlaIII CATG 4 cut(s) 76, 101, 118, 169
NlaIV GGNNCC 1 cut(s) 289
NspI RCATGY 1 cut(s) 169
PagI TCATGA 1 cut(s) 97
PdiI GCCGGC 2 cut(s) 564, 660
PdmI GAANNNNTTC 1 cut(s) 13
PfeI GAWTC 2 cut(s) 9, 196
PflFI GACNNNGTC 2 cut(s) 538, 634
PfoI TCCNGGA 4 cut(s) 583, 679, 700, 720
Psp124BI GAGCTC 1 cut(s) 302
Psp6I CCWGG 2 cut(s) 700, 720
PspGI CCWGG 2 cut(s) 700, 720
PspN4I GGNNCC 1 cut(s) 289
PspPI GGNCC 4 cut(s) 118, 191, 287, 587
PstI CTGCAG 1 cut(s) 91
PsuI RGATCY 1 cut(s) 335
PsyI GACNNNGTC 2 cut(s) 538, 634
RsaI GTAC 1 cut(s) 692
RsaNI GTAC 1 cut(s) 691
Rsr2I CGGWCCG 1 cut(s) 587
RsrII CGGWCCG 1 cut(s) 587
SacI GAGCTC 1 cut(s) 302
SaqAI TTAA 2 cut(s) 525, 621
Sau3AI GATC 3 cut(s) 75, 94, 335
Sau96I GGNCC 4 cut(s) 118, 191, 287, 587
ScrFI CCNGG 4 cut(s) 585, 681, 702, 722
SduI GDGCHC 2 cut(s) 302, 322
SfcI CTRYAG 1 cut(s) 87
SgrAI CRCCGGYG 2 cut(s) 562, 658
SinI GGWCC 3 cut(s) 118, 191, 587
SmlI CTYRAG 2 cut(s) 122, 321
SmoI CTYRAG 2 cut(s) 122, 321
Sse9I AATT 1 cut(s) 213
SsiI CCGC 6 cut(s) 505, 513, 550, 601, 609, 646
SspI AATATT 1 cut(s) 45
SspMI CTAG 2 cut(s) 6, 284
SstI GAGCTC 1 cut(s) 302
StyD4I CCNGG 4 cut(s) 583, 679, 700, 720
StyI CCWWGG 2 cut(s) 114, 283
TaaI ACNGT 3 cut(s) 407, 439, 690
TaiI ACGT 3 cut(s) 357, 487, 512
TaqI TCGA 3 cut(s) 267, 302, 444
TasI AATT 1 cut(s) 213
TatI WGTACW 1 cut(s) 690
TfiI GAWTC 2 cut(s) 9, 196
Tru1I TTAA 2 cut(s) 525, 621
Tru9I TTAA 2 cut(s) 525, 621
TspDTI ATGAA 1 cut(s) 376
TspGWI ACGGA 1 cut(s) 310
Tth111I GACNNNGTC 2 cut(s) 538, 634
VpaK11BI GGWCC 3 cut(s) 118, 191, 587
XceI RCATGY 1 cut(s) 169
XcmI CCANNNNNNNNNTGG 2 cut(s) 245, 421
XmaJI CCTAGG 1 cut(s) 283
XmnI GAANNNNTTC 1 cut(s) 13
XspI CTAG 2 cut(s) 6, 284
ZraI GACGTC 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.