FvH4_5g20110

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000118
Physical Location & Seq
Reverse (-)
11855882 .. 11858361
2480 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20110.t1

Sequence Viewer

Length: 873 bp
ATGTCGCCGGAAACTGGAATTCGGCGGCCTCGCCGAGGATCACCGAGGAGGAAAAGTGATCGGGGACGCTGCAGGAGTCCGCTGGGGAGGAGGCACGCCGTCGTCGGCGCCGGACGGTGCCGAACGGAGCTTCGTGCGCACTACGGACAGGATGAGAAGCGGACGTCCGCACTTCGCTTAAAGTGTGGACATCCGTCCGTTCTCCACCCTCCGGCGCCGACGACAGCGCACCTCCATCCCAGAACACTCCAGCAGCGTCCTCGACCACTTTTCCTCCTCGGTGAGTCCGTTCTTTTCCAGTTTCCAGCAAGATTGATCTTGTTTGAAGTTTTGGGTGATCACGCCGAAAAACTGGAAAAAAATGGACTCGCCGGGGAGGGAAAGTGGTCGAGGACGCTACTGAAGTCTTCTGGGGTGGAGGCGCGCCGTCGCCGTGCCGGACGGTGGAGAACAGACGGACGTCCACACTTTAAGCCCGGTGCGGACGTCCTTACGGATTTCCGCTTTTTCTCCACCATTCCAGAGCCAGCGACGCCGTTTCTTCTCCTCGACGTGATCCCAAACCTCCCCCGACGGTGTTGGAATGAAGAAGAAGGCCGGAAAGATCGCGATCGGAGGTCTACCATGATCGGTTGTGAAGTTCTGAGTGAGATTGCTGCACAGACCTCCGATCGCGATCTCTCCGGCCTTCTTCTTCATTCCAACACCGTCGGGGGAGGTCTGGGATCACGCCGGGGAGAAGAAACGGTGTCGCCGGCGCTAGAATGGTGGAGAAATGGCAGAAAACCCGTACGGATGTCGGCACCAGAAACAGACTATAGATTCATGATTAAGATTTCCTACATGTATCATGATGAAATCGACAAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

291

Amino Acids

33.04

Weight (kDa)

10.47

Isoelectric Point (pI)

64.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000118)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01361 FvH4_1g07161 FvH4_1g07431 FvH4_1g07821 FvH4_1g08223 FvH4_1g10041 FvH4_1g12132 FvH4_1g15053 FvH4_1g18161 FvH4_1g18400 FvH4_1g18931 FvH4_1g19242 FvH4_1g19461 FvH4_1g20550 FvH4_1g21381 FvH4_1g21542 FvH4_1g22102 FvH4_1g23991 FvH4_1g24542 FvH4_1g24951 FvH4_1g24960 FvH4_1g25272 FvH4_1g25552 FvH4_1g25771 FvH4_1g28771 FvH4_1g29201 FvH4_1g29611 FvH4_2g01181 FvH4_2g02491 FvH4_2g04621 FvH4_2g04631 FvH4_2g07460 FvH4_2g08540 FvH4_2g08550 FvH4_2g09292 FvH4_2g10501 FvH4_2g12101 FvH4_2g12220 FvH4_2g12551 FvH4_2g14071 FvH4_2g14241 FvH4_2g15041 FvH4_2g15311 FvH4_2g18971 FvH4_2g19220 FvH4_2g19260 FvH4_2g19611 FvH4_2g19652 FvH4_2g20211 FvH4_2g20231 FvH4_2g21541 FvH4_2g22380 FvH4_2g27712 FvH4_2g28171 FvH4_2g29879 FvH4_2g32391 FvH4_3g02171 FvH4_3g05531 FvH4_3g09750 FvH4_3g10310 FvH4_3g10460 FvH4_3g13172 FvH4_3g15232 FvH4_3g18481 FvH4_3g18621 FvH4_3g20504 FvH4_3g20812 FvH4_3g22320 FvH4_3g22930 FvH4_3g22940 FvH4_3g23752 FvH4_3g24791 FvH4_3g26411 FvH4_3g26432 FvH4_3g27201 FvH4_3g27862 FvH4_3g28871 FvH4_3g28954 FvH4_3g29131 FvH4_3g29832 FvH4_3g29922 FvH4_3g30190 FvH4_3g32231 FvH4_3g32250 FvH4_3g33460 FvH4_3g34670 FvH4_3g36982 FvH4_3g37001 FvH4_3g38252 FvH4_3g38772 FvH4_3g40141 FvH4_3g40181 FvH4_3g42521 FvH4_4g00090 FvH4_4g00290 FvH4_4g00301 FvH4_4g00530 FvH4_4g00823 FvH4_4g02300 FvH4_4g02450 FvH4_4g03360 FvH4_4g04481 FvH4_4g04761 FvH4_4g06301 FvH4_4g06630 FvH4_4g08312 FvH4_4g08552 FvH4_4g10053 FvH4_4g11971 FvH4_4g13211 FvH4_4g13511 FvH4_4g15351 FvH4_4g16360 FvH4_4g16601 FvH4_4g18462 FvH4_4g22911 FvH4_4g23221 FvH4_4g24301 FvH4_4g24721 FvH4_4g27905 FvH4_4g28971 FvH4_4g29540 FvH4_4g30720 FvH4_4g31521 FvH4_4g33371 FvH4_4g34111 FvH4_4g35740 FvH4_5g00131 FvH4_5g02530 FvH4_5g02550 FvH4_5g02560 FvH4_5g02580 FvH4_5g03783 FvH4_5g05681 FvH4_5g05681 FvH4_5g09371 FvH4_5g09381 FvH4_5g14611 FvH4_5g15341 FvH4_5g19801 FvH4_5g20110 FvH4_5g20911 FvH4_5g22230 FvH4_5g25751 FvH4_5g26070 FvH4_5g26471 FvH4_5g27072 FvH4_5g27471 FvH4_5g27471 FvH4_5g27998 FvH4_5g28291 FvH4_5g30951 FvH4_5g31082 FvH4_5g31890 FvH4_5g32251 FvH4_5g34112 FvH4_5g37184 FvH4_5g37993 FvH4_5g38271 FvH4_6g00153 FvH4_6g00640 FvH4_6g05921 FvH4_6g08591 FvH4_6g09511 FvH4_6g10331 FvH4_6g10401 FvH4_6g14161 FvH4_6g14171 FvH4_6g18481 FvH4_6g19721 FvH4_6g19980 FvH4_6g20104 FvH4_6g20112 FvH4_6g21261 FvH4_6g21651 FvH4_6g22051 FvH4_6g23141 FvH4_6g24822 FvH4_6g25082 FvH4_6g25391 FvH4_6g25560 FvH4_6g26350 FvH4_6g27031 FvH4_6g27810 FvH4_6g27971 FvH4_6g28331 FvH4_6g29391 FvH4_6g30781 FvH4_6g32221 FvH4_6g34680 FvH4_6g36181 FvH4_6g36551 FvH4_6g37491 FvH4_6g37663 FvH4_6g39590 FvH4_6g40582 FvH4_6g43843 FvH4_6g50551 FvH4_7g00240 FvH4_7g00731 FvH4_7g01031 FvH4_7g03301 FvH4_7g08180 FvH4_7g08180 FvH4_7g10631 FvH4_7g19710 FvH4_7g23892 FvH4_7g25280 FvH4_7g25551 FvH4_7g30121 FvH4_7g31931 FvH4_7g32672 FvH4_c21g00040 FvH4_c2g00053
prunus_persica Prupe.1G448800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322451 RchiOBHm_Chr5g0015171
rosa_laevigata RLG00000003077 RLG00000018186 RLG00000030343
rosa_multiflora Rmu_co8096726.1_g000001
rosa_roxburghii Rroxscaffold_157G00438380 Rroxscaffold_1G00001730 Rroxscaffold_3G00236310
rosa_rugosa Rorug02G0236500
rosa_samantha Rh4BG247300 Rh6BG286200 Rh6CG287300 Rh7DG257500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 167, 463, 489
Acc16I TGCGCA 1 cut(s) 138
AccB1I GGYRCC 4 cut(s) 107, 117, 214, 802
AccI GTMKAC 1 cut(s) 620
AccII CGCG 3 cut(s) 424, 609, 675
AciI CCGC 6 cut(s) 25, 80, 160, 168, 482, 502
AclWI GGATC 3 cut(s) 46, 550, 733
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 422
AcyI GRCGYC 6 cut(s) 108, 164, 215, 460, 486, 533
AfaI GTAC 1 cut(s) 792
AfiI CCNNNNNNNGG 4 cut(s) 14, 35, 211, 444
AflIII ACRYGT 1 cut(s) 843
AgsI TTSAA 1 cut(s) 326
AjiI CACGTC 1 cut(s) 553
AluBI AGCT 1 cut(s) 130
AluI AGCT 1 cut(s) 130
AlwI GGATC 3 cut(s) 46, 550, 733
AoxI GGCC 3 cut(s) 26, 595, 685
ApeKI GCWGC 3 cut(s) 69, 253, 656
ApoI RAATTY 1 cut(s) 18
AscI GGCGCGCC 1 cut(s) 422
AspLEI GCGC 7 cut(s) 110, 139, 217, 229, 424, 426, 760
AsuC2I CCSGG 3 cut(s) 373, 477, 734
AsuHPI GGTGA 3 cut(s) 33, 293, 347
BanI GGYRCC 4 cut(s) 107, 117, 214, 802
BbsI GAAGAC 1 cut(s) 399
BbvI GCAGC 3 cut(s) 56, 265, 643
BccI CCATC 1 cut(s) 243
BceAI ACGGC 4 cut(s) 83, 411, 417, 520
BcgI CGANNNNNNTGC 2 cut(s) 242, 276
BclI TGATCA 1 cut(s) 337
BcnI CCSGG 3 cut(s) 373, 477, 734
BfaI CTAG 1 cut(s) 761
BfmI CTRYAG 2 cut(s) 70, 817
BfoI RGCGCY 3 cut(s) 111, 218, 761
BisI GCNGC 4 cut(s) 26, 70, 254, 657
BlsI GCNGC 4 cut(s) 27, 71, 255, 658
Bme1390I CCNGG 3 cut(s) 373, 477, 734
BmgBI CACGTC 1 cut(s) 553
BmiI GGNNCC 4 cut(s) 109, 119, 216, 804
BmrFI CCNGG 3 cut(s) 373, 477, 734
BoxI GACNNNNGTC 2 cut(s) 193, 459
BpiI GAAGAC 1 cut(s) 399
BpmI CTGGAG 1 cut(s) 233
BpuMI CCSGG 3 cut(s) 373, 477, 734
BsaBI GATNNNNATC 2 cut(s) 609, 675
BsaHI GRCGYC 6 cut(s) 108, 164, 215, 460, 486, 533
BsaJI CCNNGG 5 cut(s) 34, 44, 277, 372, 733
BsaXI ACNNNNNCTCC 6 cut(s) 40, 70, 258, 288, 368, 398
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 35, 211, 444
Bse118I RCCGGY 1 cut(s) 754
Bse1I ACTGG 3 cut(s) 19, 298, 357
Bse8I GATNNNNATC 2 cut(s) 609, 675
BseDI CCNNGG 5 cut(s) 34, 44, 277, 372, 733
BseGI GGATG 4 cut(s) 157, 190, 235, 801
BseJI GATNNNNATC 2 cut(s) 609, 675
BseLI CCNNNNNNNGG 4 cut(s) 14, 35, 211, 444
BseMII CTCAG 1 cut(s) 635
BseNI ACTGG 3 cut(s) 19, 298, 357
BsePI GCGCGC 1 cut(s) 422
BseRI GAGGAG 4 cut(s) 61, 103, 266, 536
BseXI GCAGC 3 cut(s) 56, 265, 643
BseYI CCCAGC 1 cut(s) 82
BsgI GTGCAG 1 cut(s) 642
Bsh1236I CGCG 3 cut(s) 424, 609, 675
Bsh1285I CGRYCG 2 cut(s) 613, 673
BshFI GGCC 3 cut(s) 28, 597, 687
BshNI GGYRCC 4 cut(s) 107, 117, 214, 802
BsiEI CGRYCG 2 cut(s) 613, 673
BsiWI CGTACG 1 cut(s) 790
BslFI GGGAC 1 cut(s) 78
BslI CCNNNNNNNGG 4 cut(s) 14, 35, 211, 444
BsmFI GGGAC 1 cut(s) 78
BsnI GGCC 3 cut(s) 28, 597, 687
Bsp68I TCGCGA 2 cut(s) 609, 675
BspACI CCGC 6 cut(s) 25, 80, 160, 168, 482, 502
BspANI GGCC 3 cut(s) 28, 597, 687
BspCNI CTCAG 1 cut(s) 636
BspFNI CGCG 3 cut(s) 424, 609, 675
BspHI TCATGA 2 cut(s) 825, 850
BspLI GGNNCC 4 cut(s) 109, 119, 216, 804
BspMAI CTGCAG 1 cut(s) 74
BspPI GGATC 3 cut(s) 46, 550, 733
BspT107I GGYRCC 4 cut(s) 107, 117, 214, 802
BsrFI RCCGGY 1 cut(s) 754
BsrI ACTGG 3 cut(s) 19, 298, 357
BssAI RCCGGY 1 cut(s) 754
BssECI CCNNGG 5 cut(s) 34, 44, 277, 372, 733
BssHII GCGCGC 1 cut(s) 422
BssNI GRCGYC 6 cut(s) 108, 164, 215, 460, 486, 533
Bst4CI ACNGT 5 cut(s) 117, 444, 576, 709, 748
BstACI GRCGYC 6 cut(s) 108, 164, 215, 460, 486, 533
BstC8I GCNNGC 4 cut(s) 96, 424, 528, 756
BstDEI CTNAG 1 cut(s) 644
BstENI CCTNNNNNAGG 1 cut(s) 33
BstF5I GGATG 4 cut(s) 157, 190, 235, 801
BstFNI CGCG 3 cut(s) 424, 609, 675
BstH2I RGCGCY 3 cut(s) 111, 218, 761
BstHHI GCGC 7 cut(s) 110, 139, 217, 229, 424, 426, 760
BstMCI CGRYCG 2 cut(s) 613, 673
BstMWI GCNNNNNNNGC 2 cut(s) 136, 532
BstNSI RCATGY 1 cut(s) 847
BstPAI GACNNNNGTC 2 cut(s) 193, 459
BstSCI CCNGG 3 cut(s) 371, 475, 732
BstSFI CTRYAG 2 cut(s) 70, 817
BstUI CGCG 3 cut(s) 424, 609, 675
BstV1I GCAGC 3 cut(s) 56, 265, 643
BstV2I GAAGAC 1 cut(s) 399
BsuRI GGCC 3 cut(s) 28, 597, 687
BtrI CACGTC 1 cut(s) 553
BtsCI GGATG 4 cut(s) 157, 190, 235, 801
BtuMI TCGCGA 2 cut(s) 609, 675
Cac8I GCNNGC 4 cut(s) 96, 424, 528, 756
CciI TCATGA 2 cut(s) 825, 850
CfoI GCGC 7 cut(s) 110, 139, 217, 229, 424, 426, 760
Cfr10I RCCGGY 1 cut(s) 754
CseI GACGC 4 cut(s) 75, 245, 403, 541
Csp6I GTAC 1 cut(s) 791
CviAII CATG 4 cut(s) 625, 826, 844, 851
CviJI RGCY 6 cut(s) 28, 130, 475, 526, 597, 687
CviKI_1 RGCY 6 cut(s) 28, 130, 475, 526, 597, 687
CviQI GTAC 1 cut(s) 791
DdeI CTNAG 1 cut(s) 644
DinI GGCGCC 2 cut(s) 109, 216
Eco57I CTGAAG 1 cut(s) 422
EcoNI CCTNNNNNAGG 1 cut(s) 33
EcoRI GAATTC 1 cut(s) 18
EgeI GGCGCC 2 cut(s) 109, 216
EheI GGCGCC 2 cut(s) 109, 216
FaeI CATG 4 cut(s) 628, 829, 847, 854
FaiI YATR 5 cut(s) 626, 819, 827, 845, 852
FaqI GGGAC 1 cut(s) 78
FatI CATG 4 cut(s) 624, 825, 843, 850
FbaI TGATCA 1 cut(s) 337
FblI GTMKAC 1 cut(s) 620
Fnu4HI GCNGC 4 cut(s) 26, 70, 254, 657
FokI GGATG 4 cut(s) 164, 177, 222, 808
Fsp4HI GCNGC 4 cut(s) 26, 70, 254, 657
FspAI RTGCGCAY 1 cut(s) 138
FspBI CTAG 1 cut(s) 761
FspI TGCGCA 1 cut(s) 138
GlaI GCGC 7 cut(s) 109, 138, 216, 228, 423, 425, 759
GluI GCNGC 4 cut(s) 26, 70, 254, 657
GsaI CCCAGC 1 cut(s) 86
GsuI CTGGAG 1 cut(s) 233
HaeII RGCGCY 3 cut(s) 111, 218, 761
HaeIII GGCC 3 cut(s) 28, 597, 687
HgaI GACGC 4 cut(s) 75, 245, 403, 541
HhaI GCGC 7 cut(s) 110, 139, 217, 229, 424, 426, 760
Hin1I GRCGYC 6 cut(s) 108, 164, 215, 460, 486, 533
Hin1II CATG 4 cut(s) 628, 829, 847, 854
Hin6I GCGC 7 cut(s) 108, 137, 215, 227, 422, 424, 758
HinP1I GCGC 7 cut(s) 108, 137, 215, 227, 422, 424, 758
HinfI GANTC 4 cut(s) 76, 284, 366, 822
HphI GGTGA 3 cut(s) 33, 293, 347
Hpy166II GTNNAC 3 cut(s) 188, 464, 621
Hpy188I TCNGA 3 cut(s) 615, 645, 670
Hpy188III TCNNGA 5 cut(s) 521, 608, 674, 826, 851
Hpy8I GTNNAC 3 cut(s) 188, 464, 621
Hpy99I CGWCG 8 cut(s) 104, 107, 223, 432, 535, 554, 576, 713
HpyAV CCTTC 2 cut(s) 587, 698
HpyCH4III ACNGT 5 cut(s) 117, 444, 576, 709, 748
HpyCH4IV ACGT 4 cut(s) 164, 460, 486, 552
HpyCH4V TGCA 2 cut(s) 72, 659
HpyF10VI GCNNNNNNNGC 2 cut(s) 136, 532
HpyF3I CTNAG 1 cut(s) 644
HpySE526I ACGT 4 cut(s) 164, 460, 486, 552
Hsp92I GRCGYC 6 cut(s) 108, 164, 215, 460, 486, 533
Hsp92II CATG 4 cut(s) 628, 829, 847, 854
HspAI GCGC 7 cut(s) 108, 137, 215, 227, 422, 424, 758
KasI GGCGCC 2 cut(s) 107, 214
KroI GCCGGC 1 cut(s) 754
KroNI GCCGGC 1 cut(s) 756
Ksp22I TGATCA 1 cut(s) 337
LmnI GCTCC 1 cut(s) 127
Lsp1109I GCAGC 3 cut(s) 56, 265, 643
MaeI CTAG 1 cut(s) 761
MaeII ACGT 4 cut(s) 164, 460, 486, 552
MboII GAAGA 7 cut(s) 399, 533, 599, 602, 683, 686, 752
MluCI AATT 1 cut(s) 18
Mly113I GGCGCC 2 cut(s) 108, 215
MlyI GAGTC 3 cut(s) 85, 293, 360
MmeI TCCRAC 2 cut(s) 560, 726
MreI CGCCGGCG 1 cut(s) 754
MroNI GCCGGC 1 cut(s) 754
MseI TTAA 3 cut(s) 179, 471, 831
MspA1I CMGCKG 1 cut(s) 82
MspR9I CCNGG 3 cut(s) 373, 477, 734
MvnI CGCG 3 cut(s) 424, 609, 675
MwoI GCNNNNNNNGC 2 cut(s) 136, 532
NaeI GCCGGC 1 cut(s) 756
NarI GGCGCC 2 cut(s) 108, 215
NciI CCSGG 3 cut(s) 373, 477, 734
NgoMIV GCCGGC 1 cut(s) 754
NlaIII CATG 4 cut(s) 628, 829, 847, 854
NlaIV GGNNCC 4 cut(s) 109, 119, 216, 804
NmeAIII GCCGAG 1 cut(s) 59
NruI TCGCGA 2 cut(s) 609, 675
NsbI TGCGCA 1 cut(s) 138
NspI RCATGY 1 cut(s) 847
PagI TCATGA 2 cut(s) 825, 850
PalAI GGCGCGCC 1 cut(s) 422
PauI GCGCGC 1 cut(s) 422
PciI ACATGT 1 cut(s) 843
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PdiI GCCGGC 1 cut(s) 756
PfeI GAWTC 1 cut(s) 822
Pfl23II CGTACG 1 cut(s) 790
PkrI GCNGC 4 cut(s) 27, 71, 255, 658
Ple19I CGATCG 2 cut(s) 613, 673
PleI GAGTC 3 cut(s) 84, 292, 360
PluTI GGCGCC 2 cut(s) 111, 218
PpsI GAGTC 3 cut(s) 84, 292, 360
PscI ACATGT 1 cut(s) 843
PshAI GACNNNNGTC 2 cut(s) 193, 459
PspFI CCCAGC 1 cut(s) 82
PspLI CGTACG 1 cut(s) 790
PspN4I GGNNCC 4 cut(s) 109, 119, 216, 804
PstI CTGCAG 1 cut(s) 74
PteI GCGCGC 1 cut(s) 422
PvuI CGATCG 2 cut(s) 613, 673
RruI TCGCGA 2 cut(s) 609, 675
RsaI GTAC 1 cut(s) 792
RsaNI GTAC 1 cut(s) 791
SaqAI TTAA 3 cut(s) 179, 471, 831
SatI GCNGC 4 cut(s) 26, 70, 254, 657
SchI GAGTC 3 cut(s) 85, 293, 360
ScrFI CCNGG 3 cut(s) 373, 477, 734
SfcI CTRYAG 2 cut(s) 70, 817
SfoI GGCGCC 2 cut(s) 109, 216
SgrAI CRCCGGYG 1 cut(s) 754
SgsI GGCGCGCC 1 cut(s) 422
Sse9I AATT 1 cut(s) 18
SsiI CCGC 6 cut(s) 25, 80, 160, 168, 482, 502
SspDI GGCGCC 2 cut(s) 107, 214
SspMI CTAG 1 cut(s) 761
StyD4I CCNGG 3 cut(s) 371, 475, 732
TaaI ACNGT 5 cut(s) 117, 444, 576, 709, 748
TaiI ACGT 4 cut(s) 167, 463, 489, 555
TaqI TCGA 4 cut(s) 262, 389, 549, 861
TasI AATT 1 cut(s) 18
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 1 cut(s) 822
Tru1I TTAA 3 cut(s) 179, 471, 831
Tru9I TTAA 3 cut(s) 179, 471, 831
TseI GCWGC 3 cut(s) 69, 253, 656
TspDTI ATGAA 4 cut(s) 600, 686, 814, 870
TspGWI ACGGA 8 cut(s) 140, 159, 183, 187, 277, 471, 509, 808
XagI CCTNNNNNAGG 1 cut(s) 33
XapI RAATTY 1 cut(s) 18
XceI RCATGY 1 cut(s) 847
XmiI GTMKAC 1 cut(s) 620
XspI CTAG 1 cut(s) 761
ZraI GACGTC 3 cut(s) 165, 461, 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.