FvH4_3g32250

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000118
Physical Location & Seq
Forward (+)
27540630 .. 27546492
5863 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g32250.t1

Sequence Viewer

Length: 933 bp
ATGAGAGACAACTTGGTGAGCCTTGAATGCCTAAGGATCTCGTCGCCGGAGTTAGATGGCTTGCCGCTGCACAAGTTCAAGGGGCTGAAGGAGCTGGAGCTCTCCAAAGTCACGTCCCGGTGCTCGGCGGTTCCGATTCTGATCCAGGTTTCTAGGCTCAAGCTCCTAACAAAGCTATCAGTATGTTATTTCTCTATAACTTTTCTACCACCGGAGATTTGCTGCTTGAAGAGCTTGGAGTATCTGGACCTTTCGTTCAACAAGATGAAGACTTTGCCCGGAGAAATTGGGAATTTGAATGCTCTGGTGCTACTACTTAGTACTTACTACAAAGCTGCTACTGCCCAACTAGGAAGTTCAACAAAGCTGCTACTGCCTGATTGTGAGTTCAACAACGTTGCTACTGCTCGACTGGGAGTTCACAACGCTGCTACTGCCCGATTTTCACAATGCTGCTACTGCCCGATTGTGAGTTCAACAATCCTGCTACTGCCCAACTGGGAGTTCACCAACGCTGCTATTGCCCAACTAAGAGTTTACCAACGCTGCTACTGCCCAACTAAGAGTTCACCAACGCTGCTACTGCCCAATTGTGAGTTCAACAGTCCTGCTACTGCCCAACTGGGAGGCTTAATGGTCTATCAAAGGCGAAGCTTTGGTGGTGGTATTGCCTCCAACACGGAGGAGATTGGGATTCTTCTAGATCTCGCCGGCTGGCAGAGTCAAGCTCGGCTTCGTCGGAATCTTACAAGGCAGCGCCAAAGTGGTTTATCGTCATCGTTGTCGAGGTTGTCATTGACGGCTTTGGCGAACGAGGAGCAACCAAATCATGACAGAGAGACGGTGGATCTAAGGCGTCGACGCGGTACAGCCATTAGAGCACCTCCGATCTCTCCGCCAAGAAGGCCGCTGCTCAAGAAGTGCAGACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

311

Amino Acids

34.47

Weight (kDa)

9.62

Isoelectric Point (pI)

61.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 16 - 122 4e-09 Leucine-rich repeat region
LRR_8 PF13855 56 - 105 1e-05 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000118)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01361 FvH4_1g07161 FvH4_1g07431 FvH4_1g07821 FvH4_1g08223 FvH4_1g10041 FvH4_1g12132 FvH4_1g15053 FvH4_1g18161 FvH4_1g18400 FvH4_1g18931 FvH4_1g19242 FvH4_1g19461 FvH4_1g20550 FvH4_1g21381 FvH4_1g21542 FvH4_1g22102 FvH4_1g23991 FvH4_1g24542 FvH4_1g24951 FvH4_1g24960 FvH4_1g25272 FvH4_1g25552 FvH4_1g25771 FvH4_1g28771 FvH4_1g29201 FvH4_1g29611 FvH4_2g01181 FvH4_2g02491 FvH4_2g04621 FvH4_2g04631 FvH4_2g07460 FvH4_2g08540 FvH4_2g08550 FvH4_2g09292 FvH4_2g10501 FvH4_2g12101 FvH4_2g12220 FvH4_2g12551 FvH4_2g14071 FvH4_2g14241 FvH4_2g15041 FvH4_2g15311 FvH4_2g18971 FvH4_2g19220 FvH4_2g19260 FvH4_2g19611 FvH4_2g19652 FvH4_2g20211 FvH4_2g20231 FvH4_2g21541 FvH4_2g22380 FvH4_2g27712 FvH4_2g28171 FvH4_2g29879 FvH4_2g32391 FvH4_3g02171 FvH4_3g05531 FvH4_3g09750 FvH4_3g10310 FvH4_3g10460 FvH4_3g13172 FvH4_3g15232 FvH4_3g18481 FvH4_3g18621 FvH4_3g20504 FvH4_3g20812 FvH4_3g22320 FvH4_3g22930 FvH4_3g22940 FvH4_3g23752 FvH4_3g24791 FvH4_3g26411 FvH4_3g26432 FvH4_3g27201 FvH4_3g27862 FvH4_3g28871 FvH4_3g28954 FvH4_3g29131 FvH4_3g29832 FvH4_3g29922 FvH4_3g30190 FvH4_3g32231 FvH4_3g32250 FvH4_3g33460 FvH4_3g34670 FvH4_3g36982 FvH4_3g37001 FvH4_3g38252 FvH4_3g38772 FvH4_3g40141 FvH4_3g40181 FvH4_3g42521 FvH4_4g00090 FvH4_4g00290 FvH4_4g00301 FvH4_4g00530 FvH4_4g00823 FvH4_4g02300 FvH4_4g02450 FvH4_4g03360 FvH4_4g04481 FvH4_4g04761 FvH4_4g06301 FvH4_4g06630 FvH4_4g08312 FvH4_4g08552 FvH4_4g10053 FvH4_4g11971 FvH4_4g13211 FvH4_4g13511 FvH4_4g15351 FvH4_4g16360 FvH4_4g16601 FvH4_4g18462 FvH4_4g22911 FvH4_4g23221 FvH4_4g24301 FvH4_4g24721 FvH4_4g27905 FvH4_4g28971 FvH4_4g29540 FvH4_4g30720 FvH4_4g31521 FvH4_4g33371 FvH4_4g34111 FvH4_4g35740 FvH4_5g00131 FvH4_5g02530 FvH4_5g02550 FvH4_5g02560 FvH4_5g02580 FvH4_5g03783 FvH4_5g05681 FvH4_5g05681 FvH4_5g09371 FvH4_5g09381 FvH4_5g14611 FvH4_5g15341 FvH4_5g19801 FvH4_5g20110 FvH4_5g20911 FvH4_5g22230 FvH4_5g25751 FvH4_5g26070 FvH4_5g26471 FvH4_5g27072 FvH4_5g27471 FvH4_5g27471 FvH4_5g27998 FvH4_5g28291 FvH4_5g30951 FvH4_5g31082 FvH4_5g31890 FvH4_5g32251 FvH4_5g34112 FvH4_5g37184 FvH4_5g37993 FvH4_5g38271 FvH4_6g00153 FvH4_6g00640 FvH4_6g05921 FvH4_6g08591 FvH4_6g09511 FvH4_6g10331 FvH4_6g10401 FvH4_6g14161 FvH4_6g14171 FvH4_6g18481 FvH4_6g19721 FvH4_6g19980 FvH4_6g20104 FvH4_6g20112 FvH4_6g21261 FvH4_6g21651 FvH4_6g22051 FvH4_6g23141 FvH4_6g24822 FvH4_6g25082 FvH4_6g25391 FvH4_6g25560 FvH4_6g26350 FvH4_6g27031 FvH4_6g27810 FvH4_6g27971 FvH4_6g28331 FvH4_6g29391 FvH4_6g30781 FvH4_6g32221 FvH4_6g34680 FvH4_6g36181 FvH4_6g36551 FvH4_6g37491 FvH4_6g37663 FvH4_6g39590 FvH4_6g40582 FvH4_6g43843 FvH4_6g50551 FvH4_7g00240 FvH4_7g00731 FvH4_7g01031 FvH4_7g03301 FvH4_7g08180 FvH4_7g08180 FvH4_7g10631 FvH4_7g19710 FvH4_7g23892 FvH4_7g25280 FvH4_7g25551 FvH4_7g30121 FvH4_7g31931 FvH4_7g32672 FvH4_c21g00040 FvH4_c2g00053
prunus_persica Prupe.1G448800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322451 RchiOBHm_Chr5g0015171
rosa_laevigata RLG00000003077 RLG00000018186 RLG00000030343
rosa_multiflora Rmu_co8096726.1_g000001
rosa_roxburghii Rroxscaffold_157G00438380 Rroxscaffold_1G00001730 Rroxscaffold_3G00236310
rosa_rugosa Rorug02G0236500
rosa_samantha Rh4BG247300 Rh6BG286200 Rh6CG287300 Rh7DG257500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 859
AccII CGCG 1 cut(s) 864
AciI CCGC 5 cut(s) 65, 128, 864, 896, 908
AclI AACGTT 1 cut(s) 396
AclWI GGATC 3 cut(s) 44, 136, 855
AcsI RAATTY 1 cut(s) 292
AcuI CTGAAG 1 cut(s) 107
AcyI GRCGYC 1 cut(s) 856
AfaI GTAC 2 cut(s) 322, 868
AfiI CCNNNNNNNGG 1 cut(s) 124
AgsI TTSAA 9 cut(s) 26, 79, 229, 259, 298, 360, 391, 477, 601
AjiI CACGTC 1 cut(s) 114
AjnI CCWGG 1 cut(s) 144
AjuI GAANNNNNNNTTGG 6 cut(s) 488, 520, 550, 581, 582, 613
AloI GAACNNNNNNTCC 2 cut(s) 239, 271
AluBI AGCT 9 cut(s) 94, 100, 163, 175, 234, 335, 367, 654, 728
AluI AGCT 9 cut(s) 94, 100, 163, 175, 234, 335, 367, 654, 728
Alw21I GWGCWC 3 cut(s) 102, 125, 883
Alw26I GTCTC 1 cut(s) 833
AlwI GGATC 3 cut(s) 44, 136, 855
AoxI GGCC 1 cut(s) 905
ApoI RAATTY 1 cut(s) 292
AspLEI GCGC 1 cut(s) 759
AspS9I GGNCC 1 cut(s) 247
AsuC2I CCSGG 2 cut(s) 118, 279
AsuHPI GGTGA 3 cut(s) 28, 499, 561
AvaII GGWCC 1 cut(s) 247
AxyI CCTNAGG 1 cut(s) 32
BanII GRGCYC 1 cut(s) 102
BbsI GAAGAC 1 cut(s) 275
Bbv12I GWGCWC 3 cut(s) 102, 125, 883
BccI CCATC 1 cut(s) 50
BceAI ACGGC 1 cut(s) 816
BciT130I CCWGG 1 cut(s) 146
BcnI CCSGG 2 cut(s) 118, 279
BcoDI GTCTC 1 cut(s) 833
BfaI CTAG 3 cut(s) 153, 350, 701
BfoI RGCGCY 1 cut(s) 760
BglI GCCNNNNNGGC 1 cut(s) 904
BglII AGATCT 1 cut(s) 703
BmcAI AGTACT 1 cut(s) 322
Bme1390I CCNGG 3 cut(s) 118, 146, 279
Bme18I GGWCC 1 cut(s) 247
BmgBI CACGTC 1 cut(s) 114
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 1 cut(s) 132
BmrFI CCNGG 3 cut(s) 118, 146, 279
BmrI ACTGGG 3 cut(s) 422, 508, 632
BmuI ACTGGG 3 cut(s) 422, 508, 632
BpiI GAAGAC 1 cut(s) 275
BplI GAGNNNNNCTC 2 cut(s) 712, 744
BpmI CTGGAG 1 cut(s) 116
BpuEI CTTGAG 2 cut(s) 143, 899
BpuMI CCSGG 2 cut(s) 118, 279
BsaBI GATNNNNATC 1 cut(s) 140
BsaHI GRCGYC 1 cut(s) 856
BsaWI WCCGGW 1 cut(s) 211
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse118I RCCGGY 1 cut(s) 710
Bse1I ACTGG 3 cut(s) 417, 503, 627
Bse21I CCTNAGG 1 cut(s) 32
Bse8I GATNNNNATC 1 cut(s) 140
BseBI CCWGG 1 cut(s) 146
BseJI GATNNNNATC 1 cut(s) 140
BseLI CCNNNNNNNGG 1 cut(s) 124
BseNI ACTGG 3 cut(s) 417, 503, 627
BseRI GAGGAG 2 cut(s) 698, 830
BsgI GTGCAG 1 cut(s) 53
Bsh1236I CGCG 1 cut(s) 864
BshFI GGCC 1 cut(s) 907
BsiHKAI GWGCWC 3 cut(s) 102, 125, 883
BsiSI CCGG 5 cut(s) 47, 118, 212, 279, 711
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 1 cut(s) 833
BsmBI CGTCTC 1 cut(s) 833
BsmFI GGGAC 1 cut(s) 100
BsmI GAATGC 2 cut(s) 32, 304
BsnI GGCC 1 cut(s) 907
Bsp1286I GDGCHC 3 cut(s) 102, 125, 883
Bsp143I GATC 5 cut(s) 36, 141, 703, 847, 888
BspACI CCGC 5 cut(s) 65, 128, 864, 896, 908
BspANI GGCC 1 cut(s) 907
BspFNI CGCG 1 cut(s) 864
BspHI TCATGA 1 cut(s) 829
BspLI GGNNCC 1 cut(s) 132
BspPI GGATC 3 cut(s) 44, 136, 855
BspQI GCTCTTC 1 cut(s) 224
BsrFI RCCGGY 1 cut(s) 710
BsrI ACTGG 3 cut(s) 417, 503, 627
BssAI RCCGGY 1 cut(s) 710
BssMI GATC 5 cut(s) 36, 141, 703, 847, 888
BssNI GRCGYC 1 cut(s) 856
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 3 cut(s) 605, 844, 930
Bst6I CTCTTC 1 cut(s) 224
BstACI GRCGYC 1 cut(s) 856
BstC8I GCNNGC 3 cut(s) 62, 712, 716
BstDEI CTNAG 5 cut(s) 32, 317, 530, 561, 851
BstFNI CGCG 1 cut(s) 864
BstH2I RGCGCY 1 cut(s) 760
BstHHI GCGC 1 cut(s) 759
BstKTI GATC 5 cut(s) 39, 144, 706, 850, 891
BstMAI GTCTC 1 cut(s) 833
BstMBI GATC 5 cut(s) 36, 141, 703, 847, 888
BstNI CCWGG 1 cut(s) 146
BstSCI CCNGG 3 cut(s) 116, 144, 277
BstUI CGCG 1 cut(s) 864
BstV2I GAAGAC 1 cut(s) 275
BstX2I RGATCY 3 cut(s) 36, 703, 847
BstYI RGATCY 3 cut(s) 36, 703, 847
Bsu36I CCTNAGG 1 cut(s) 32
BsuRI GGCC 1 cut(s) 907
BtrI CACGTC 1 cut(s) 114
Cac8I GCNNGC 3 cut(s) 62, 712, 716
CciI TCATGA 1 cut(s) 829
CfoI GCGC 1 cut(s) 759
Cfr10I RCCGGY 1 cut(s) 710
Cfr13I GGNCC 1 cut(s) 247
CseI GACGC 2 cut(s) 845, 870
Csp6I GTAC 2 cut(s) 321, 867
CviAII CATG 1 cut(s) 830
CviQI GTAC 2 cut(s) 321, 867
DdeI CTNAG 5 cut(s) 32, 317, 530, 561, 851
DpnI GATC 5 cut(s) 38, 143, 705, 849, 890
DpnII GATC 5 cut(s) 36, 141, 703, 847, 888
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
EciI GGCGGA 1 cut(s) 885
Ecl136II GAGCTC 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 107
Eco81I CCTNAGG 1 cut(s) 32
EcoICRI GAGCTC 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 102
Esp3I CGTCTC 1 cut(s) 833
FaeI CATG 1 cut(s) 833
FaiI YATR 3 cut(s) 184, 197, 831
FalI AAGNNNNNCTT 2 cut(s) 717, 749
FaqI GGGAC 1 cut(s) 100
FatI CATG 1 cut(s) 829
FblI GTMKAC 1 cut(s) 859
FriOI GRGCYC 1 cut(s) 102
FspBI CTAG 3 cut(s) 153, 350, 701
GlaI GCGC 1 cut(s) 758
GsuI CTGGAG 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 760
HaeIII GGCC 1 cut(s) 907
HapII CCGG 5 cut(s) 47, 118, 212, 279, 711
HgaI GACGC 2 cut(s) 845, 870
HhaI GCGC 1 cut(s) 759
Hin1I GRCGYC 1 cut(s) 856
Hin1II CATG 1 cut(s) 833
Hin6I GCGC 1 cut(s) 757
HinP1I GCGC 1 cut(s) 757
HincII GTYRAC 1 cut(s) 860
HindII GTYRAC 1 cut(s) 860
HindIII AAGCTT 1 cut(s) 652
HinfI GANTC 4 cut(s) 136, 694, 721, 742
HpaII CCGG 5 cut(s) 47, 118, 212, 279, 711
HphI GGTGA 3 cut(s) 28, 499, 561
Hpy166II GTNNAC 5 cut(s) 421, 507, 538, 569, 860
Hpy188I TCNGA 4 cut(s) 135, 141, 741, 888
Hpy188III TCNNGA 4 cut(s) 245, 701, 830, 916
Hpy8I GTNNAC 5 cut(s) 421, 507, 538, 569, 860
Hpy99I CGWCG 4 cut(s) 46, 741, 861, 864
HpyAV CCTTC 2 cut(s) 82, 897
HpyCH4III ACNGT 3 cut(s) 605, 844, 930
HpyCH4IV ACGT 2 cut(s) 113, 396
HpyCH4V TGCA 2 cut(s) 70, 924
HpyF3I CTNAG 5 cut(s) 32, 317, 530, 561, 851
HpySE526I ACGT 2 cut(s) 113, 396
Hsp92I GRCGYC 1 cut(s) 856
Hsp92II CATG 1 cut(s) 833
HspAI GCGC 1 cut(s) 757
KroI GCCGGC 1 cut(s) 710
KroNI GCCGGC 1 cut(s) 712
Kzo9I GATC 5 cut(s) 36, 141, 703, 847, 888
LguI GCTCTTC 1 cut(s) 224
LmnI GCTCC 4 cut(s) 91, 97, 168, 817
MaeI CTAG 3 cut(s) 153, 350, 701
MaeII ACGT 2 cut(s) 113, 396
MaeIII GTNAC 1 cut(s) 109
MalI GATC 5 cut(s) 38, 143, 705, 849, 890
MboI GATC 5 cut(s) 36, 141, 703, 847, 888
MboII GAAGA 3 cut(s) 241, 280, 689
MfeI CAATTG 1 cut(s) 589
MflI RGATCY 3 cut(s) 36, 703, 847
MhlI GDGCHC 3 cut(s) 102, 125, 883
MluCI AATT 3 cut(s) 285, 292, 589
MlyI GAGTC 1 cut(s) 730
MmeI TCCRAC 2 cut(s) 699, 719
MnlI CCTC 6 cut(s) 620, 676, 682, 780, 808, 894
MroNI GCCGGC 1 cut(s) 710
MseI TTAA 1 cut(s) 632
MspA1I CMGCKG 2 cut(s) 67, 910
MspI CCGG 5 cut(s) 47, 118, 212, 279, 711
MspR9I CCNGG 3 cut(s) 118, 146, 279
MunI CAATTG 1 cut(s) 589
Mva1269I GAATGC 2 cut(s) 32, 304
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 1 cut(s) 864
NaeI GCCGGC 1 cut(s) 712
NciI CCSGG 2 cut(s) 118, 279
NdeII GATC 5 cut(s) 36, 141, 703, 847, 888
NgoMIV GCCGGC 1 cut(s) 710
NlaIII CATG 1 cut(s) 833
NlaIV GGNNCC 1 cut(s) 132
NmeAIII GCCGAG 2 cut(s) 104, 709
NmuCI GTSAC 1 cut(s) 109
PagI TCATGA 1 cut(s) 829
PciSI GCTCTTC 1 cut(s) 224
PcsI WCGNNNNNNNCGW 2 cut(s) 131, 806
PctI GAATGC 2 cut(s) 32, 304
PdiI GCCGGC 1 cut(s) 712
PfeI GAWTC 3 cut(s) 136, 694, 742
PleI GAGTC 1 cut(s) 729
PpsI GAGTC 1 cut(s) 729
Psp124BI GAGCTC 1 cut(s) 102
Psp1406I AACGTT 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 132
PspPI GGNCC 1 cut(s) 247
PsuI RGATCY 3 cut(s) 36, 703, 847
RsaI GTAC 2 cut(s) 322, 868
RsaNI GTAC 2 cut(s) 321, 867
SacI GAGCTC 1 cut(s) 102
SalI GTCGAC 1 cut(s) 858
SapI GCTCTTC 1 cut(s) 224
SaqAI TTAA 1 cut(s) 632
Sau3AI GATC 5 cut(s) 36, 141, 703, 847, 888
Sau96I GGNCC 1 cut(s) 247
ScaI AGTACT 1 cut(s) 322
SchI GAGTC 1 cut(s) 730
ScrFI CCNGG 3 cut(s) 118, 146, 279
SduI GDGCHC 3 cut(s) 102, 125, 883
SgrDI CGTCGACG 1 cut(s) 858
SinI GGWCC 1 cut(s) 247
SmlI CTYRAG 2 cut(s) 158, 914
SmoI CTYRAG 2 cut(s) 158, 914
Sse9I AATT 3 cut(s) 285, 292, 589
SsiI CCGC 5 cut(s) 65, 128, 864, 896, 908
SspMI CTAG 3 cut(s) 153, 350, 701
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 3 cut(s) 116, 144, 277
TaaI ACNGT 3 cut(s) 605, 844, 930
TaiI ACGT 2 cut(s) 116, 399
TaqI TCGA 3 cut(s) 409, 785, 859
TasI AATT 3 cut(s) 285, 292, 589
TatI WGTACW 1 cut(s) 320
TauI GCSGC 2 cut(s) 67, 910
TfiI GAWTC 3 cut(s) 136, 694, 742
Tru1I TTAA 1 cut(s) 632
Tru9I TTAA 1 cut(s) 632
TseFI GTSAC 1 cut(s) 109
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 1 cut(s) 281
TspGWI ACGGA 1 cut(s) 695
VpaK11BI GGWCC 1 cut(s) 247
XapI RAATTY 1 cut(s) 292
XbaI TCTAGA 1 cut(s) 700
XmiI GTMKAC 1 cut(s) 859
XspI CTAG 3 cut(s) 153, 350, 701
ZrmI AGTACT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.